##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211430_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10234417 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.144733696115765 31.0 31.0 34.0 28.0 34.0 2 31.191768715306402 31.0 31.0 34.0 28.0 34.0 3 31.253261714858795 31.0 31.0 34.0 27.0 34.0 4 34.87822296081936 37.0 35.0 37.0 32.0 37.0 5 34.93127473699772 37.0 35.0 37.0 32.0 37.0 6 34.99355762033147 37.0 35.0 37.0 32.0 37.0 7 34.916979442991234 37.0 35.0 37.0 32.0 37.0 8 34.9175793794605 37.0 35.0 37.0 32.0 37.0 9 36.455542997710566 39.0 37.0 39.0 32.0 39.0 10-11 36.36827754819839 39.0 37.0 39.0 32.0 39.0 12-13 36.2634406043842 39.0 35.0 39.0 31.0 39.0 14-15 37.41054307245835 40.0 37.0 41.0 31.0 41.0 16-17 37.39813376765868 40.0 37.0 41.0 31.0 41.0 18-19 37.39739322718627 40.0 37.0 41.0 31.0 41.0 20-21 37.382412549732926 40.0 37.0 41.0 31.0 41.0 22-23 37.26199997518178 40.0 36.5 41.0 31.0 41.0 24-25 37.16602108356538 40.0 36.0 41.0 31.0 41.0 26-27 37.028224958979095 40.0 36.0 41.0 30.0 41.0 28-29 36.89244946732188 39.0 36.0 41.0 30.0 41.0 30-31 36.73186997363895 39.0 36.0 41.0 30.0 41.0 32-33 36.52773220985621 39.0 35.0 41.0 30.0 41.0 34-35 36.413082738371905 39.0 35.0 41.0 29.0 41.0 36-37 36.293126076453596 39.0 35.0 40.5 29.0 41.0 38-39 36.141333404726424 39.0 35.0 40.0 28.0 41.0 40-41 35.980996328369265 38.5 35.0 40.0 27.5 41.0 42-43 35.794014402579066 38.0 35.0 40.0 27.0 41.0 44-45 35.66234305285782 38.0 34.5 40.0 27.0 41.0 46-47 35.444371232870424 38.0 34.0 40.0 26.0 41.0 48-49 35.20712235000782 38.0 34.0 40.0 25.0 41.0 50-51 34.54780643587222 37.5 33.0 39.5 24.5 40.5 52-53 34.8584532465308 38.0 33.5 39.5 25.5 40.5 54-55 35.27253467393404 38.0 34.0 40.0 26.0 41.0 56-57 35.20279450211966 38.0 34.0 40.0 26.0 41.0 58-59 35.004017327025075 38.0 34.0 40.0 25.5 41.0 60-61 34.78665604499015 37.5 33.5 40.0 25.0 41.0 62-63 34.505870388122744 37.0 33.0 40.0 25.0 41.0 64-65 34.30645277596174 37.0 33.0 40.0 25.0 41.0 66-67 34.124423208473914 36.0 33.0 39.5 24.5 41.0 68-69 33.80596637795783 36.0 33.0 39.0 24.0 41.0 70-71 33.46279094353885 35.0 32.0 39.0 23.5 41.0 72-73 33.105453686321354 35.0 32.0 38.0 23.0 40.0 74-75 32.75956793630746 35.0 32.0 37.0 22.5 39.5 76-77 32.4120945042595 35.0 31.0 37.0 22.0 39.0 78-79 32.059222327954785 35.0 31.0 36.0 21.0 39.0 80-81 31.713503758934195 35.0 31.0 36.0 20.0 38.0 82-83 31.391247298209564 35.0 30.5 35.5 20.0 37.0 84-85 31.095987734328197 34.0 30.0 35.0 19.5 37.0 86-87 30.805319101224818 34.0 30.0 35.0 18.5 36.0 88-89 30.548332797070906 34.0 29.5 35.0 18.0 36.0 90-91 30.296018131760704 34.0 29.0 35.0 17.5 36.0 92-93 30.054926675354345 34.0 29.0 35.0 17.0 35.0 94-95 29.821733714778283 34.0 29.0 35.0 15.0 35.0 96-97 29.568802990927573 34.0 29.0 35.0 13.5 35.0 98-99 29.302503357054924 34.0 27.5 35.0 10.5 35.0 100 28.076787666556875 32.0 25.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.4207613288834331 1101 2 -0.4072373785630852 1101 3 -0.5295991375865938 1101 4 -0.431526555151585 1101 5 -0.37280182858985 1101 6 -0.4029523795302694 1101 7 -0.38348839610377183 1101 8 -0.4065457018407912 1101 9 -0.5799794357834429 1101 10-11 -0.5205913531657771 1101 12-13 -0.5861264429209925 1101 14-15 -0.7250886793778477 1101 16-17 -0.7395022594229843 1101 18-19 -0.7401099342422199 1101 20-21 -0.80300276045012 1101 22-23 -0.7484538811032877 1101 24-25 -0.7989297848155417 1101 26-27 -0.8343634694206443 1101 28-29 -0.9135566031151754 1101 30-31 -0.9901483401590454 1101 32-33 -0.9452126279467521 1101 34-35 -0.9693159757606651 1101 36-37 -0.9288477418555843 1101 38-39 -0.9875963486323514 1101 40-41 -1.1670737826704922 1101 42-43 -1.1239946813328245 1101 44-45 -1.2045760361977784 1101 46-47 -1.1331857382750883 1101 48-49 -1.204117379072521 1101 50-51 -1.0952484840767696 1101 52-53 -0.8875462416174642 1101 54-55 -0.8705718418862958 1101 56-57 -0.9430616904501363 1101 58-59 -0.9957563667295091 1101 60-61 -0.9945227678025574 1101 62-63 -1.0612261092410051 1101 64-65 -0.9886555249125522 1101 66-67 -1.0072390406629665 1101 68-69 -1.1113284103212422 1101 70-71 -1.08200645148829 1101 72-73 -1.129675140811063 1101 74-75 -1.2272043301317765 1101 76-77 -1.1356459725506554 1101 78-79 -1.1755497812323732 1101 80-81 -1.3571408496002881 1101 82-83 -1.321527725009883 1101 84-85 -1.285212693224178 1101 86-87 -1.3495919073176204 1101 88-89 -1.4753105514490628 1101 90-91 -1.5931885661428282 1101 92-93 -1.7201408515784884 1101 94-95 -1.7900772712419446 1101 96-97 -1.780397534085477 1101 98-99 -1.8953049956691288 1101 100 -1.8979853569482756 1102 1 -0.41933373717843736 1102 2 -0.3296106268810597 1102 3 -0.4445102848133118 1102 4 -0.45100551181807447 1102 5 -0.330378413001128 1102 6 -0.30740111828173156 1102 7 -0.3904857742053949 1102 8 -0.40023709635720195 1102 9 -0.4675457963968128 1102 10-11 -0.49153238061520454 1102 12-13 -0.5504036252891567 1102 14-15 -0.7797545641223707 1102 16-17 -0.7140020262929951 1102 18-19 -0.6798629379303023 1102 20-21 -0.7339120731070352 1102 22-23 -0.6992613030863168 1102 24-25 -0.7619398760685101 1102 26-27 -0.8008163991054786 1102 28-29 -0.8054838690395059 1102 30-31 -0.7800749566926797 1102 32-33 -0.7771245781954903 1102 34-35 -0.8620163126723597 1102 36-37 -0.872002597643224 1102 38-39 -0.9592387142464247 1102 40-41 -1.0052599988671673 1102 42-43 -1.075508665889636 1102 44-45 -1.0948986737297446 1102 46-47 -1.1314001093855879 1102 48-49 -1.2120846906388962 1102 50-51 -0.9841326766150829 1102 52-53 -0.8265792340914615 1102 54-55 -0.8289903853326237 1102 56-57 -0.8732968386110755 1102 58-59 -0.8143677330726646 1102 60-61 -0.8699901586114294 1102 62-63 -0.8685195785394839 1102 64-65 -0.9501574472187286 1102 66-67 -0.9357787635713635 1102 68-69 -0.8792158872534515 1102 70-71 -0.8857915783761072 1102 72-73 -0.8908989748972047 1102 74-75 -0.9837140146965133 1102 76-77 -0.9236209453254176 1102 78-79 -1.0254915651357912 1102 80-81 -1.078540233001366 1102 82-83 -1.1008069485378371 1102 84-85 -1.2279526230677114 1102 86-87 -1.2760270090264854 1102 88-89 -1.2740661362918715 1102 90-91 -1.353246763768599 1102 92-93 -1.3389503598736816 1102 94-95 -1.3274972937960214 1102 96-97 -1.4865082605227826 1102 98-99 -1.5701743284778367 1102 100 -1.5547820294297416 1103 1 -0.3573150326252943 1103 2 -0.4204614405097118 1103 3 -0.4585213463206763 1103 4 -0.3377785756965892 1103 5 -0.2945239091941545 1103 6 -0.28921385590750504 1103 7 -0.32305686862115124 1103 8 -0.347884780083092 1103 9 -0.4715480774317058 1103 10-11 -0.4361790876212268 1103 12-13 -0.5264535444148137 1103 14-15 -0.6323072301189399 1103 16-17 -0.6454926606553144 1103 18-19 -0.6913715499754787 1103 20-21 -0.6730474724843418 1103 22-23 -0.6608368359541998 1103 24-25 -0.626906084559117 1103 26-27 -0.7074312753952938 1103 28-29 -0.739723479879423 1103 30-31 -0.7343438669393478 1103 32-33 -0.7681477521084119 1103 34-35 -0.8130178417116483 1103 36-37 -0.7899106533303879 1103 38-39 -0.8063001239418099 1103 40-41 -0.7617150550012894 1103 42-43 -0.7722561422394705 1103 44-45 -0.878891053761663 1103 46-47 -0.9063506671518411 1103 48-49 -0.9125436136271077 1103 50-51 -0.8129179709276855 1103 52-53 -0.6371493718901888 1103 54-55 -0.6909405496318115 1103 56-57 -0.6929031000804642 1103 58-59 -0.6139482301959447 1103 60-61 -0.6455937238533664 1103 62-63 -0.6538298343273325 1103 64-65 -0.6886766810200271 1103 66-67 -0.7121352645330603 1103 68-69 -0.6598060064511841 1103 70-71 -0.6926770110789278 1103 72-73 -0.7032291994248112 1103 74-75 -0.7532523366790826 1103 76-77 -0.8123490522731487 1103 78-79 -0.847176313476627 1103 80-81 -0.8327383299951627 1103 82-83 -0.8526074759740325 1103 84-85 -0.9265930567770226 1103 86-87 -1.0541547492216523 1103 88-89 -1.0626484287494584 1103 90-91 -1.0778448515210144 1103 92-93 -1.0254345893371628 1103 94-95 -1.10061528643174 1103 96-97 -1.2239688692262263 1103 98-99 -1.2488135372145877 1103 100 -1.3879594788233547 1104 1 -0.4990743249739431 1104 2 -0.48047988537507536 1104 3 -0.5003893283222887 1104 4 -0.33480815685754806 1104 5 -0.35225450007427384 1104 6 -0.398597445280771 1104 7 -0.39173860342518196 1104 8 -0.48737972624548576 1104 9 -0.5856991372406171 1104 10-11 -0.5094267992029771 1104 12-13 -0.5250957593526948 1104 14-15 -0.7128010357337331 1104 16-17 -0.7922779968948461 1104 18-19 -0.7786361752089377 1104 20-21 -0.8376834854826996 1104 22-23 -0.7857504099505803 1104 24-25 -0.7860948126558398 1104 26-27 -0.7933138307522469 1104 28-29 -0.8969163946090788 1104 30-31 -0.922848257230477 1104 32-33 -1.0256557034117506 1104 34-35 -0.9946423588906228 1104 36-37 -1.0339987903095604 1104 38-39 -0.9340766234813032 1104 40-41 -0.9483200805025049 1104 42-43 -1.0221181264222707 1104 44-45 -1.0158590595362469 1104 46-47 -1.078123541852861 1104 48-49 -1.0723936496565685 1104 50-51 -1.0216837405629704 1104 52-53 -0.853121410436323 1104 54-55 -0.8050487995927327 1104 56-57 -0.7795265642802462 1104 58-59 -0.8932874649406202 1104 60-61 -0.8477392113519002 1104 62-63 -0.9250716251751356 1104 64-65 -0.8989078646827195 1104 66-67 -0.8613155718796435 1104 68-69 -0.9045282681579891 1104 70-71 -0.8118346708935746 1104 72-73 -0.8407804600860302 1104 74-75 -0.8889197666914264 1104 76-77 -0.9404510904276862 1104 78-79 -0.865013114960604 1104 80-81 -0.8559751109437279 1104 82-83 -0.903432712570595 1104 84-85 -0.9460443482413545 1104 86-87 -0.9986998329188523 1104 88-89 -1.1021059548372882 1104 90-91 -1.0304021664271588 1104 92-93 -1.1201621760661062 1104 94-95 -1.129352238662868 1104 96-97 -1.2162226732869925 1104 98-99 -1.3300070451893262 1104 100 -1.2187388221775137 1105 1 -0.5452964541714138 1105 2 -0.5122776772587763 1105 3 -0.5253069009759095 1105 4 -0.3969011609028499 1105 5 -0.445143991664267 1105 6 -0.4665910120703103 1105 7 -0.5298730370636591 1105 8 -0.48164558504704047 1105 9 -0.6351311927019268 1105 10-11 -0.5589707273829063 1105 12-13 -0.6162407408811745 1105 14-15 -0.8037632216612778 1105 16-17 -0.722617580688123 1105 18-19 -0.7516574647142775 1105 20-21 -0.8422436098667276 1105 22-23 -0.793842203432888 1105 24-25 -0.874602465983294 1105 26-27 -0.9131785287302918 1105 28-29 -0.8741362858230346 1105 30-31 -0.9156525625780958 1105 32-33 -0.9811720520386089 1105 34-35 -0.9936124866758647 1105 36-37 -0.9633949404370199 1105 38-39 -0.9989132606795721 1105 40-41 -0.995499259994773 1105 42-43 -0.9917237703856401 1105 44-45 -0.8858616670383839 1105 46-47 -1.0288599304418256 1105 48-49 -1.134038627804408 1105 50-51 -1.0359554434617664 1105 52-53 -0.8082946388267587 1105 54-55 -0.808912353444704 1105 56-57 -0.8176290788000955 1105 58-59 -0.7399634935296646 1105 60-61 -0.8233836127331955 1105 62-63 -0.8484229209743503 1105 64-65 -0.7600979674629897 1105 66-67 -0.8133411248126805 1105 68-69 -0.8000770238914825 1105 70-71 -0.7612392990775874 1105 72-73 -0.7926272683064539 1105 74-75 -0.7716710606680373 1105 76-77 -0.8669259738911563 1105 78-79 -0.9239120082567354 1105 80-81 -0.8977224901395076 1105 82-83 -0.9288158551721537 1105 84-85 -0.8793407277525596 1105 86-87 -0.8627305432382997 1105 88-89 -0.9707156740883853 1105 90-91 -1.0135720012432188 1105 92-93 -1.0241381200215685 1105 94-95 -1.067052939160817 1105 96-97 -1.0302363657598974 1105 98-99 -1.0733222827909792 1105 100 -1.1305860125572096 1106 1 -0.44947536604104954 1106 2 -0.4689595803008082 1106 3 -0.47773715494277624 1106 4 -0.3295706239808993 1106 5 -0.3823532600380659 1106 6 -0.41765088995889244 1106 7 -0.4887432916163448 1106 8 -0.4629723123237284 1106 9 -0.6071628602943306 1106 10-11 -0.6264248317170455 1106 12-13 -0.5715259498312761 1106 14-15 -0.6925526837296445 1106 16-17 -0.7303554353181525 1106 18-19 -0.7454283784250464 1106 20-21 -0.7190679890328795 1106 22-23 -0.7372009735794975 1106 24-25 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46-47 -1.1116090490929977 1107 48-49 -1.1522359451813173 1107 50-51 -1.1809435173359049 1107 52-53 -1.0057294754753414 1107 54-55 -0.9807379425929597 1107 56-57 -0.9225646094798705 1107 58-59 -0.929835712545831 1107 60-61 -0.9262913740022967 1107 62-63 -0.8651035910567586 1107 64-65 -0.8681292404733654 1107 66-67 -0.8839229391366672 1107 68-69 -0.8571051656009772 1107 70-71 -0.8531421765296727 1107 72-73 -0.899971923139617 1107 74-75 -0.8276995268783978 1107 76-77 -0.8900485506160649 1107 78-79 -1.0016284415268863 1107 80-81 -0.9703553986883513 1107 82-83 -0.9883213445531887 1107 84-85 -0.9659031550685917 1107 86-87 -1.0224428963771004 1107 88-89 -1.0298498992812064 1107 90-91 -1.0364543886618236 1107 92-93 -1.138153052063931 1107 94-95 -1.1236121173751457 1107 96-97 -1.1867452890333787 1107 98-99 -1.1284598184133365 1107 100 -1.0491227659552358 1108 1 -0.4858588841344371 1108 2 -0.512942464241668 1108 3 -0.573964336304762 1108 4 -0.39992851164898724 1108 5 -0.3579707143454556 1108 6 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0.8743658866541182 2115 74-75 0.8351919161467407 2115 76-77 0.844761625523951 2115 78-79 0.8566386808114963 2115 80-81 0.9291379113600406 2115 82-83 0.9182422391542708 2115 84-85 0.9444216949522151 2115 86-87 0.836783668968188 2115 88-89 0.8830908897782166 2115 90-91 0.9968590929031791 2115 92-93 1.1315198387836816 2115 94-95 1.213796353421646 2115 96-97 1.272068893941448 2115 98-99 1.3381582762320754 2115 100 1.3778352691526976 2116 1 0.2957779587232423 2116 2 0.27484058417551793 2116 3 0.3059575834979391 2116 4 0.17916822289498668 2116 5 0.15535418457350403 2116 6 0.15900352145415297 2116 7 0.1663768611001828 2116 8 0.1298416690309594 2116 9 0.19796935777529256 2116 10-11 0.22470298438103242 2116 12-13 0.2668007934095371 2116 14-15 0.5042291577849625 2116 16-17 0.46582128253619004 2116 18-19 0.5049837645240061 2116 20-21 0.4644792070497914 2116 22-23 0.46500693338370525 2116 24-25 0.2949431563088254 2116 26-27 0.32742088511550804 2116 28-29 0.4227270917824413 2116 30-31 0.568827894597213 2116 32-33 0.4870990473638912 2116 34-35 0.5545238991347432 2116 36-37 0.7109607034702847 2116 38-39 0.7367132996584544 2116 40-41 0.644315723878627 2116 42-43 0.6665989316814986 2116 44-45 0.6171289010995054 2116 46-47 0.5879221149201186 2116 48-49 0.6163819851980676 2116 50-51 0.5358028066087357 2116 52-53 0.3749912510215907 2116 54-55 0.41562380279783184 2116 56-57 0.48423757844382465 2116 58-59 0.3836823990738125 2116 60-61 0.5322917316584181 2116 62-63 0.3655714948329418 2116 64-65 0.26686421092293955 2116 66-67 0.4095271479387108 2116 68-69 0.4116115132562683 2116 70-71 0.21369508321029684 2116 72-73 0.34011100902235825 2116 74-75 0.49514900278872176 2116 76-77 0.6361039998826001 2116 78-79 0.6852525029975816 2116 80-81 0.7838816717101658 2116 82-83 0.6220976302443297 2116 84-85 0.776510787114244 2116 86-87 0.8726569565364386 2116 88-89 0.9353089390620397 2116 90-91 0.987069185755189 2116 92-93 1.0506304274929654 2116 94-95 1.1416884307908113 2116 96-97 1.1637298641134421 2116 98-99 1.2496861382278404 2116 100 1.1627328885833883 2201 1 0.7307709771050064 2201 2 0.7452406480871048 2201 3 0.8171596351620138 2201 4 0.6378810545845894 2201 5 0.5987736793831431 2201 6 0.6434130404318381 2201 7 0.7072634634471413 2201 8 0.7388088112788225 2201 9 0.8888898970205688 2201 10-11 0.928798039646388 2201 12-13 0.60595753386864 2201 14-15 0.8829644160347101 2201 16-17 1.033631849467163 2201 18-19 1.0495428525064412 2201 20-21 1.0509214115102878 2201 22-23 1.0788686796611486 2201 24-25 1.0456496247170648 2201 26-27 1.0142438366087916 2201 28-29 0.9875207157038446 2201 30-31 0.9788107217279034 2201 32-33 1.0092311485779462 2201 34-35 1.016585675518094 2201 36-37 0.5062688001871223 2201 38-39 0.80739372532787 2201 40-41 0.8433590992800575 2201 42-43 0.8770318681586886 2201 44-45 0.9561114542312055 2201 46-47 0.9727817138467927 2201 48-49 0.9588998810664933 2201 50-51 1.0788513401663167 2201 52-53 1.0212588167238792 2201 54-55 0.9879890921115688 2201 56-57 0.9616968197851179 2201 58-59 0.9491857538083792 2201 60-61 0.8754691531548104 2201 62-63 0.8570477234521334 2201 64-65 0.8784546601386083 2201 66-67 0.7873951944291377 2201 68-69 0.280099482360157 2201 70-71 0.621315244431031 2201 72-73 0.673781445580758 2201 74-75 0.6610319680919474 2201 76-77 0.6289673349584106 2201 78-79 0.5629440856472847 2201 80-81 0.5253799914712509 2201 82-83 0.509554655588115 2201 84-85 0.4473141688816007 2201 86-87 0.3943241650491274 2201 88-89 0.3720571958228547 2201 90-91 0.36287122649486747 2201 92-93 0.3439488802445858 2201 94-95 0.2697940986665479 2201 96-97 0.237517845192869 2201 98-99 0.21399366811796128 2201 100 0.24728732573873557 2202 1 0.8303655594476034 2202 2 0.8235709614510256 2202 3 0.9148076995230348 2202 4 0.7947505272115976 2202 5 0.8152100216922378 2202 6 0.8099357925384751 2202 7 0.8645875739641866 2202 8 0.8807865355680704 2202 9 0.975598331836494 2202 10-11 1.035781538888024 2202 12-13 1.0872006291812895 2202 14-15 1.3079522674950326 2202 16-17 1.2442638564229256 2202 18-19 1.2319541090376092 2202 20-21 1.2722608286849137 2202 22-23 0.7904454051383141 2202 24-25 0.9821768111276441 2202 26-27 1.1935983760197217 2202 28-29 1.232432983808181 2202 30-31 1.2702631661176653 2202 32-33 1.271662866905892 2202 34-35 1.242380149820427 2202 36-37 1.248321552868056 2202 38-39 1.2261211195177069 2202 40-41 1.2024375617259437 2202 42-43 0.49503887925929035 2202 44-45 0.9454817274212814 2202 46-47 1.1242882448414306 2202 48-49 1.1479936150788959 2202 50-51 1.268237953113264 2202 52-53 1.2341103678684462 2202 54-55 1.2187333417576909 2202 56-57 1.112990524287028 2202 58-59 1.1318290209744575 2202 60-61 1.0740483363200042 2202 62-63 1.084718023486083 2202 64-65 1.1003051777974022 2202 66-67 0.99923527674936 2202 68-69 1.0070058987600774 2202 70-71 0.9513643858247249 2202 72-73 0.8620388800735128 2202 74-75 0.7942673427535283 2202 76-77 0.8087622284984022 2202 78-79 0.7545468071056725 2202 80-81 0.7657717390747187 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1.0586095230727892 2203 42-43 1.122603210508096 2203 44-45 1.0887268964993453 2203 46-47 1.0701496267510464 2203 48-49 1.06020246247936 2203 50-51 1.1776471283751775 2203 52-53 1.109328632966438 2203 54-55 1.0246427296077414 2203 56-57 0.9591259429708288 2203 58-59 0.9921438966996377 2203 60-61 1.006217870342276 2203 62-63 0.9335800617903871 2203 64-65 0.9295118744761126 2203 66-67 0.9615824294704467 2203 68-69 0.8918577225226016 2203 70-71 0.8812873166680077 2203 72-73 0.821701661307543 2203 74-75 0.8336505160996097 2203 76-77 0.7772644190975129 2203 78-79 0.7798521579604731 2203 80-81 0.7736274703501884 2203 82-83 0.7644006285634966 2203 84-85 0.741847305546024 2203 86-87 0.7708867431561188 2203 88-89 0.7450445275147501 2203 90-91 0.7547864099865436 2203 92-93 0.7631231882540774 2203 94-95 0.7775397910083086 2203 96-97 0.7510859070447786 2203 98-99 0.5715484237269166 2203 100 0.6111391241555459 2204 1 0.8296784987121804 2204 2 0.843484360047956 2204 3 0.8538608563252588 2204 4 0.7162456714695367 2204 5 0.6793241230775422 2204 6 0.7129351689086505 2204 7 0.7577802383003913 2204 8 0.7867220399181605 2204 9 0.912792435659 2204 10-11 0.9376848622458951 2204 12-13 0.9806021603849402 2204 14-15 1.1961617260321944 2204 16-17 1.1221177084875507 2204 18-19 1.1463725983979458 2204 20-21 1.1339409835131846 2204 22-23 1.1754911315847352 2204 24-25 1.1564951608925966 2204 26-27 1.1770803134918495 2204 28-29 1.1740977240014487 2204 30-31 1.198134650607905 2204 32-33 1.2050308256294286 2204 34-35 1.1869768783249128 2204 36-37 1.132280224062363 2204 38-39 1.193778497702418 2204 40-41 1.1364964101842858 2204 42-43 1.154830347138052 2204 44-45 1.231501918674745 2204 46-47 1.1752107909800031 2204 48-49 1.0999030683307964 2204 50-51 1.2007491596352438 2204 52-53 1.1767954160103429 2204 54-55 1.0590070367688824 2204 56-57 1.0867889262273422 2204 58-59 1.165826970479273 2204 60-61 1.0815224887296395 2204 62-63 1.104725477219752 2204 64-65 1.0434469642073552 2204 66-67 0.9763953886881751 2204 68-69 0.9603481589174478 2204 70-71 0.9996656362726242 2204 72-73 0.951946410515319 2204 74-75 0.9654348376710828 2204 76-77 0.893735506859322 2204 78-79 0.8566552304654742 2204 80-81 0.8336552845246494 2204 82-83 0.9105712305749911 2204 84-85 0.841918776462844 2204 86-87 0.8490530706472725 2204 88-89 0.8195445339299781 2204 90-91 0.8329158713649605 2204 92-93 0.8056943426578371 2204 94-95 0.8399762535745019 2204 96-97 0.7986592110558774 2204 98-99 0.8024457674383321 2204 100 0.8053278087790332 2205 1 0.8215196530674227 2205 2 0.8708592456494806 2205 3 0.9296512195913529 2205 4 0.7441875035842784 2205 5 0.7713339055033472 2205 6 0.8019913391342612 2205 7 0.828341331036647 2205 8 0.7993444589811602 2205 9 0.9861855893402733 2205 10-11 1.0328514839365255 2205 12-13 1.0757516379227852 2205 14-15 1.3062751259162582 2205 16-17 1.2526767319181857 2205 18-19 1.2435271838501905 2205 20-21 1.2305858221599948 2205 22-23 1.2868112486836907 2205 24-25 1.2763422895329342 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1.104944306048747 2205 94-95 1.073626812394707 2205 96-97 1.0807722935873798 2205 98-99 1.0642225339991889 2205 100 1.0384919111759991 2206 1 0.7879441086162267 2206 2 0.8038595185372159 2206 3 0.8262584288781163 2206 4 0.6893004591662617 2206 5 0.7220915245542301 2206 6 0.7987729094914826 2206 7 0.8019301500732539 2206 8 0.8005440548229856 2206 9 0.9667314051209175 2206 10-11 0.9922265287475796 2206 12-13 1.023993086379967 2206 14-15 1.2130026696813658 2206 16-17 1.235067844716319 2206 18-19 1.2409688422102718 2206 20-21 1.2133546228203116 2206 22-23 1.2146280150259088 2206 24-25 1.2461428987297651 2206 26-27 1.2698687779959883 2206 28-29 1.241729509627966 2206 30-31 1.2868929116015053 2206 32-33 1.254755018195162 2206 34-35 1.2631070115366967 2206 36-37 1.2657771750720244 2206 38-39 1.2956707079421719 2206 40-41 1.2870341719995722 2206 42-43 1.2776596807602445 2206 44-45 1.3903413353812226 2206 46-47 1.4080424588275804 2206 48-49 1.377085536946467 2206 50-51 1.4055342307630738 2206 52-53 1.3016458377851947 2206 54-55 1.2895688813706059 2206 56-57 1.2909322976309596 2206 58-59 1.2495205326613146 2206 60-61 1.218529925529488 2206 62-63 1.2631521461959778 2206 64-65 1.2325039943292708 2206 66-67 1.241193449340365 2206 68-69 1.218164998268982 2206 70-71 1.2301537206444664 2206 72-73 1.1930009502358203 2206 74-75 1.1811460288561122 2206 76-77 1.0819649151944546 2206 78-79 1.0784370944396038 2206 80-81 1.0805425551131087 2206 82-83 1.0930268065286732 2206 84-85 1.0691293054475146 2206 86-87 1.0882940944867485 2206 88-89 1.087944557483361 2206 90-91 1.124536332072541 2206 92-93 1.1248268394052552 2206 94-95 1.0799140850536446 2206 96-97 1.0864670072093787 2206 98-99 1.0836113936564864 2206 100 1.1616093293589849 2207 1 0.7364049099904584 2207 2 0.7665444147352112 2207 3 0.8269227252612303 2207 4 0.6888922872699155 2207 5 0.6897098035883005 2207 6 0.7304820309085898 2207 7 0.7934376546414725 2207 8 0.7912176993114173 2207 9 1.0313568442985144 2207 10-11 0.9810207841644285 2207 12-13 0.9730726611975058 2207 14-15 1.1409041585427673 2207 16-17 1.1338110011633233 2207 18-19 1.166943776683759 2207 20-21 1.1464488642255617 2207 22-23 1.179423232091949 2207 24-25 1.2105654149198983 2207 26-27 1.2258507258844489 2207 28-29 1.2439350523964663 2207 30-31 1.2711021548851065 2207 32-33 1.2710549583551582 2207 34-35 1.2555985080872958 2207 36-37 1.2430047835465317 2207 38-39 1.291343333294634 2207 40-41 1.2942548857989777 2207 42-43 1.3598921366533787 2207 44-45 1.3621112224298457 2207 46-47 1.3991827610671237 2207 48-49 1.4160462912488256 2207 50-51 1.3309863755839118 2207 52-53 1.27195092263036 2207 54-55 1.211274952208079 2207 56-57 1.2300045524086514 2207 58-59 1.2444822964825661 2207 60-61 1.20748941768165 2207 62-63 1.2301992411740272 2207 64-65 1.2128094954827588 2207 66-67 1.1903803644252093 2207 68-69 1.1275667219722507 2207 70-71 1.1221593094972633 2207 72-73 1.1149617403757688 2207 74-75 1.1113051241813565 2207 76-77 1.0744161142220463 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66-67 1.2130128931221407 2311 68-69 1.2128702607673674 2311 70-71 1.2013464098895312 2311 72-73 1.2265937234315416 2311 74-75 1.1553083916302285 2311 76-77 1.164786479801613 2311 78-79 1.1387486555490476 2311 80-81 1.1586499540261137 2311 82-83 1.2259595747502168 2311 84-85 1.2269300215793386 2311 86-87 1.2604012095236072 2311 88-89 1.2901901636226967 2311 90-91 1.3527157782984176 2311 92-93 1.4506873706638004 2311 94-95 1.4373099429286782 2311 96-97 1.463671552441287 2311 98-99 1.5020369565477019 2311 100 1.6274867621010252 2312 1 0.6316065955750751 2312 2 0.6620143644485914 2312 3 0.7393427716021499 2312 4 0.6022094198477674 2312 5 0.5938393893167131 2312 6 0.46741108603686854 2312 7 0.6320695375584151 2312 8 0.6732725276036433 2312 9 0.7822833421752549 2312 10-11 0.8820238480538407 2312 12-13 0.9678623881634039 2312 14-15 1.1352594341666844 2312 16-17 1.1141718938484289 2312 18-19 1.0731753267600226 2312 20-21 1.1346914103002632 2312 22-23 1.1566343317325902 2312 24-25 1.1495264376073706 2312 26-27 1.2113977669818965 2312 28-29 1.2426606312645845 2312 30-31 1.1288952528037655 2312 32-33 1.2513691421090911 2312 34-35 1.299710139601956 2312 36-37 1.3237557832300624 2312 38-39 1.3245533417280555 2312 40-41 1.349575307817922 2312 42-43 1.3741006438234464 2312 44-45 1.3949376098287303 2312 46-47 1.328961065992516 2312 48-49 1.3813097916494215 2312 50-51 1.2980548514598311 2312 52-53 1.1764297637759498 2312 54-55 1.135877599497114 2312 56-57 1.0729972052852759 2312 58-59 1.1364965762604058 2312 60-61 1.0951693834051284 2312 62-63 1.0843353669854068 2312 64-65 1.1131985159365243 2312 66-67 1.0651716416946755 2312 68-69 1.1111994462327814 2312 70-71 1.1194078745297915 2312 72-73 1.12572005196364 2312 74-75 1.1270416003846577 2312 76-77 1.0893027126803503 2312 78-79 1.0456718328444587 2312 80-81 0.9442079922703108 2312 82-83 1.1552082344317007 2312 84-85 1.1393989125699413 2312 86-87 1.2532191119310845 2312 88-89 1.2595333718342374 2312 90-91 1.3036055459701927 2312 92-93 1.3542457746442302 2312 94-95 1.3846225912113823 2312 96-97 1.419663388141906 2312 98-99 1.3253919292746374 2312 100 1.4944643998069047 2313 1 0.6434136572537632 2313 2 0.7101261598588131 2313 3 0.7478222890549944 2313 4 0.6515671051355909 2313 5 0.6370854236010572 2313 6 0.6525975249873781 2313 7 0.7380578603965802 2313 8 0.7597449405855059 2313 9 0.902709359080248 2313 10-11 0.795796271791211 2313 12-13 0.9394450110138237 2313 14-15 1.1573225465870252 2313 16-17 1.1281519992323794 2313 18-19 1.1533287861493804 2313 20-21 1.1643291804704958 2313 22-23 1.2001173513123575 2313 24-25 1.1912874750774805 2313 26-27 1.2118485988456555 2313 28-29 1.2231936475355312 2313 30-31 1.2442027934125477 2313 32-33 1.2851667209435007 2313 34-35 1.3337013215746296 2313 36-37 1.3086384578904244 2313 38-39 1.328786228769431 2313 40-41 1.3900010762071062 2313 42-43 1.4228702899000396 2313 44-45 1.430999635239047 2313 46-47 1.4251653019045847 2313 48-49 1.4525483917300122 2313 50-51 1.4056417025654966 2313 52-53 1.3043495018729985 2313 54-55 1.2713793873041581 2313 56-57 1.2571977500011116 2313 58-59 1.2964957827395693 2313 60-61 1.3137256573772262 2313 62-63 1.3017656609704247 2313 64-65 1.2670934971564165 2313 66-67 1.2112693279746765 2313 68-69 1.2870663194364909 2313 70-71 1.242094892032938 2313 72-73 1.304918570782398 2313 74-75 1.3143340858004322 2313 76-77 1.2965265205703602 2313 78-79 1.286439903041643 2313 80-81 1.2904530022073608 2313 82-83 1.282768455696985 2313 84-85 1.3586740377714328 2313 86-87 1.3985890077099867 2313 88-89 1.406681746865857 2313 90-91 1.469399238363323 2313 92-93 1.5834319440246851 2313 94-95 1.6184030665095506 2313 96-97 1.6408540073742408 2313 98-99 1.6573566384894534 2313 100 1.7687712491525538 2314 1 0.45826277008813676 2314 2 0.4659354741016948 2314 3 0.5313115925567153 2314 4 0.43046452329829066 2314 5 0.43693258268123003 2314 6 0.469960321362656 2314 7 0.5247740484960062 2314 8 0.5227460610773278 2314 9 0.6601089603792758 2314 10-11 0.6363201233393099 2314 12-13 0.4281444921568962 2314 14-15 0.8025822735105095 2314 16-17 0.8284180674940345 2314 18-19 0.8791252636739983 2314 20-21 0.921626131755616 2314 22-23 0.8024838725976053 2314 24-25 0.8238767249941503 2314 26-27 0.9405367402838181 2314 28-29 1.0389054408384197 2314 30-31 0.9024139704661494 2314 32-33 1.0064313078897058 2314 34-35 1.055287412293758 2314 36-37 1.09477483479629 2314 38-39 1.102456824930286 2314 40-41 1.0720210122999418 2314 42-43 1.172209853097847 2314 44-45 1.1919339210518913 2314 46-47 1.2139616695437851 2314 48-49 1.2717021963583193 2314 50-51 1.211491692688206 2314 52-53 1.0236883558904992 2314 54-55 0.9891098804481686 2314 56-57 1.007291952186165 2314 58-59 1.0173931918294628 2314 60-61 1.017965445955987 2314 62-63 0.9691640387457738 2314 64-65 0.9050952320209618 2314 66-67 1.0113353734498673 2314 68-69 1.04882882536441 2314 70-71 1.0286833330336904 2314 72-73 1.0019160358462926 2314 74-75 1.0822254173163088 2314 76-77 1.047846015303712 2314 78-79 1.0843948790021294 2314 80-81 1.1185629152980106 2314 82-83 0.9669029696746385 2314 84-85 0.9513844769087392 2314 86-87 1.1295623806821382 2314 88-89 1.257852538924304 2314 90-91 1.3192299048403981 2314 92-93 1.347433769160407 2314 94-95 1.4324404665181518 2314 96-97 1.4576205897018681 2314 98-99 1.5111072476812062 2314 100 1.4274936626344363 2315 1 0.4210594612825105 2315 2 0.45910141703231844 2315 3 0.5255792700062045 2315 4 0.45228310219847145 2315 5 0.4362327027165236 2315 6 -0.3004542457797683 2315 7 0.2874348262752662 2315 8 0.34385560659599435 2315 9 0.5475704447442808 2315 10-11 0.6233873168625976 2315 12-13 0.6828301173939337 2315 14-15 0.8159987885454569 2315 16-17 0.6918591093963684 2315 18-19 0.810020631855842 2315 20-21 0.8472273711951175 2315 22-23 0.7323355809368124 2315 24-25 0.8179812621870539 2315 26-27 0.8801229970119877 2315 28-29 0.9543773174541528 2315 30-31 1.0350218666034436 2315 32-33 1.0491733359638857 2315 34-35 1.0403688158815996 2315 36-37 1.1062951100876646 2315 38-39 1.118453354454246 2315 40-41 1.1887703362340005 2315 42-43 1.2173204870593324 2315 44-45 1.1900147756870467 2315 46-47 1.2823243604691754 2315 48-49 1.3082440682882037 2315 50-51 1.2721375391061187 2315 52-53 1.1118865073671174 2315 54-55 0.867508914412582 2315 56-57 0.9203786855733114 2315 58-59 0.9012786929938557 2315 60-61 0.7935595632675856 2315 62-63 0.9283789777528568 2315 64-65 1.036898636814449 2315 66-67 1.0136698322350242 2315 68-69 1.046115868693441 2315 70-71 1.059909258790455 2315 72-73 1.099593033025954 2315 74-75 1.0380005231010472 2315 76-77 1.0493822469372631 2315 78-79 0.9917136098073875 2315 80-81 1.064074344010752 2315 82-83 1.15976935426551 2315 84-85 1.1452026906815824 2315 86-87 1.1831121432232017 2315 88-89 1.2888065968465732 2315 90-91 1.3783702759884235 2315 92-93 1.42954727985596 2315 94-95 1.4331806852311892 2315 96-97 1.4609466234323634 2315 98-99 1.6115849948480623 2315 100 1.7243975178189288 2316 1 0.060159941947137696 2316 2 -0.03776044915171184 2316 3 0.13247473559599143 2316 4 0.08823176399322108 2316 5 0.08205704878871956 2316 6 0.1566971822865355 2316 7 0.16453871152393873 2316 8 0.20410353918772017 2316 9 0.31210322957999637 2316 10-11 0.20075941926896945 2316 12-13 0.3086014174255425 2316 14-15 0.4666638945385415 2316 16-17 0.5056233064643152 2316 18-19 0.4505632895299314 2316 20-21 0.3880648465051024 2316 22-23 0.3293516997030821 2316 24-25 0.37887623798554415 2316 26-27 0.4151271871626747 2316 28-29 0.5298483975944137 2316 30-31 0.5495468066614251 2316 32-33 0.6333828427060411 2316 34-35 0.6449856210090061 2316 36-37 0.7333435003392736 2316 38-39 0.7601239897040557 2316 40-41 0.7128665114272366 2316 42-43 0.7381875092173402 2316 44-45 0.6363826782419437 2316 46-47 0.5608286325817033 2316 48-49 0.7056160627979509 2316 50-51 0.4723893737942291 2316 52-53 0.15233359981037609 2316 54-55 0.14768656175532158 2316 56-57 0.2763267272099341 2316 58-59 0.3881670255277001 2316 60-61 0.46095342268244366 2316 62-63 0.49462967239926314 2316 64-65 0.4086113037399315 2316 66-67 0.4256735374448084 2316 68-69 0.4621806074618533 2316 70-71 0.3278792936202848 2316 72-73 0.42421524387339105 2316 74-75 0.47604892750789674 2316 76-77 0.5650757742878696 2316 78-79 0.6306422353963903 2316 80-81 0.71102728633198 2316 82-83 0.6837714539932769 2316 84-85 0.7706202951745134 2316 86-87 0.8388578935314008 2316 88-89 0.8955413929744118 2316 90-91 0.9736147123334717 2316 92-93 1.0377182766089668 2316 94-95 1.0744682132125476 2316 96-97 1.1693322305369946 2316 98-99 1.2667681978772478 2316 100 1.1891670664606089 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 55.0 1 543.0 2 279.0 3 174.0 4 168.0 5 148.0 6 194.0 7 1182.0 8 4001.0 9 7288.0 10 10315.0 11 13866.0 12 18599.0 13 23048.0 14 27334.0 15 32765.0 16 39937.0 17 49908.0 18 60736.0 19 71539.0 20 85267.0 21 100320.0 22 110442.0 23 114655.0 24 116932.0 25 122469.0 26 134814.0 27 154102.0 28 180283.0 29 210896.0 30 251891.0 31 308922.0 32 385473.0 33 498726.0 34 687626.0 35 912254.0 36 1312158.0 37 1907139.0 38 1802637.0 39 474795.0 40 537.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.194538799939124 18.77602855323599 12.404755791135813 23.62467685568907 2 15.9675568911075 20.348828789051293 40.84004925728516 22.84356506255605 3 18.12784475954391 27.715654421259167 30.118563655178104 24.03793716401882 4 12.326710470724382 16.961075659975716 38.50817834612529 32.20403552317461 5 12.72124342487016 37.90875416982398 34.28161938756671 15.088383017739154 6 29.272042735018506 37.040538768913386 19.840653855715836 13.846764640352276 7 26.78186651044316 33.13242239673308 23.273935746109167 16.8117753467146 8 26.272512532679603 35.073113733712994 20.81835528462708 17.836018448980326 9 28.244327787935042 15.359341105336503 20.465211709929324 35.93111939679913 10-11 24.98108623168087 25.772397535616264 29.235238597450053 20.01127763525281 12-13 25.374055297491815 24.12115925898755 28.480381766092417 22.02440367742822 14-15 23.092530740223737 25.37795753576666 26.594739581854675 24.934772142154927 16-17 22.15835920535572 27.555786647519977 27.39934025426729 22.886513892857018 18-19 21.854271369797797 27.281400531428883 29.09952566803271 21.76480243074061 20-21 22.951240391856334 26.807090371584685 28.475380903974347 21.76628833258464 22-23 22.74203843474829 26.52927758853918 28.498576858208402 22.23010711850413 24-25 22.1308335222786 26.30779281955857 28.894093570909206 22.667280087253623 26-27 22.0934405301373 26.4913067837939 28.63950215973212 22.775750526336672 28-29 22.04532636706242 27.531240279175957 28.453425507227287 21.970007846534337 30-31 23.272081429798305 26.53194909347626 28.574099975021294 21.62186950170414 32-33 23.061212402744086 26.59010836406973 28.295930852314093 22.05274838087209 34-35 22.883687014234138 26.89695128801544 28.5449922010648 21.674369496685618 36-37 22.483165870412105 26.871667337847523 28.468100437975437 22.17706635376494 38-39 22.58594550747583 26.971314644667356 28.445596044814682 21.99714380304213 40-41 22.607389987847935 26.340338346165943 28.829307762329304 22.22296390365682 42-43 22.34857370169029 26.68899503292811 28.136986408480574 22.825444856901026 44-45 21.73535758467691 27.073947921570348 28.745028550158665 22.445665943594083 46-47 21.709763427456547 26.444748053171356 28.357316853286978 23.48817166608512 48-49 22.126071825465598 26.555578330976125 28.410201355245047 22.90814848831323 50-51 22.013306289737386 26.88378266311982 28.300424345980147 22.802486701162643 52-53 21.753662672901513 27.158495836131063 27.99879648435014 23.089045006617283 54-55 21.556517527366424 26.757460427002744 28.536614094912633 23.149407950718196 56-57 22.13654521978045 26.62893859105398 28.028851337527357 23.205664851638215 58-59 21.627348351934902 26.772325243856155 28.55167296820345 23.04865343600549 60-61 22.18554594644623 26.88029059377402 28.041821281447433 22.892342178332314 62-63 22.145034301115444 26.66834149686817 27.994490359203677 23.192133842812705 64-65 21.734165319439946 26.351656936157458 28.13478030280337 23.779397441599233 66-67 21.838427740168733 26.22511969658555 27.92624993573633 24.010202627509386 68-69 21.359293951517543 26.42572754546284 28.30309254196778 23.91188596105184 70-71 21.646940928254576 26.133992309501018 28.514066706283824 23.705000055960582 72-73 21.81510378265879 26.619726439803948 27.95804539030234 23.607124387234926 74-75 22.063849891164292 26.577511821809672 27.782733394735853 23.57590489229018 76-77 21.88935871209317 26.276388464386645 27.887298135439547 23.946954688080638 78-79 21.719665003116255 26.334435444642423 28.20993522439118 23.73596432785014 80-81 21.871575504622744 26.390577841036862 28.053324423028265 23.684522231312133 82-83 22.387001978015107 26.209633441746284 28.195173604052975 23.208190976185637 84-85 22.488973780153202 26.495287920817507 27.54570005154291 23.470038247486386 86-87 22.034623188864664 26.67863861959894 27.975823319734307 23.310914871802087 88-89 22.22411324728534 25.948978189249196 27.985910250777774 23.840998312687688 90-91 22.87875864179219 25.658444425365744 28.070384182430253 23.392412750411815 92-93 22.910374475729142 25.90143461098251 27.869550577231177 23.318640336057175 94-95 22.61086729475948 25.721225089330506 27.922872817777684 23.745034798132323 96-97 22.681440780539827 25.567906084399667 27.930881126755775 23.819772008304735 98-99 22.646867587959775 25.708534540916844 27.653375705942047 23.991222165181338 100 22.91784623639677 25.646267379564158 27.577472869285348 23.858413514753725 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1528.0 1 1153.0 2 2212.0 3 3335.5 4 2352.5 5 1794.5 6 2429.5 7 4289.0 8 6865.0 9 8264.5 10 7606.0 11 6930.5 12 7515.5 13 8394.5 14 9302.5 15 10366.0 16 11443.0 17 12437.5 18 13434.5 19 14507.5 20 15713.5 21 17291.0 22 19606.5 23 22859.5 24 27358.5 25 33569.5 26 41502.0 27 50816.0 28 63023.5 29 80046.0 30 100813.0 31 121201.5 32 146309.5 33 171242.5 34 194254.0 35 218433.0 36 244345.0 37 278242.5 38 296732.5 39 296156.0 40 296176.5 41 298559.0 42 305138.5 43 318846.0 44 337603.0 45 364684.5 46 391513.5 47 412596.5 48 434790.5 49 451612.0 50 459225.5 51 455998.5 52 435498.5 53 408229.5 54 380086.0 55 340037.5 56 300244.0 57 260285.0 58 218115.0 59 181867.5 60 145297.5 61 112731.0 62 88393.0 63 68950.5 64 51011.0 65 35955.5 66 25962.5 67 19706.5 68 15088.5 69 11275.0 70 8685.5 71 6562.0 72 4985.0 73 3725.5 74 2749.0 75 2089.0 76 1493.0 77 996.0 78 671.0 79 501.5 80 371.0 81 261.5 82 208.0 83 154.5 84 117.0 85 95.0 86 67.5 87 53.0 88 56.0 89 43.5 90 28.5 91 25.0 92 23.0 93 19.0 94 18.0 95 12.0 96 6.0 97 5.0 98 4.0 99 1.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22507388549831414 2 0.15067785492813124 3 0.023889978295783727 4 0.02282494449854838 5 0.028042633009774765 6 0.05487366793829096 7 0.06190875357140519 8 0.05431672365900275 9 0.04096960286062215 10-11 0.043339058785664096 12-13 0.04180501927955447 14-15 0.019712896201122154 16-17 0.017963895745111813 18-19 0.017739163842942888 20-21 0.020973349043721785 22-23 0.024212419720634797 24-25 0.021271363087902322 26-27 0.018545267405070558 28-29 0.018310764550633414 30-31 0.01662038980823236 32-33 0.02462279971589979 34-35 0.02512600375771282 36-37 0.023943718533258905 38-39 0.022272885695394276 40-41 0.023557765918664444 42-43 0.029674382038566535 44-45 0.03909358002512503 46-47 0.044071880205780165 48-49 0.039963194776996086 50-51 0.027256071352183518 52-53 0.04287493855292392 54-55 0.04174150808981108 56-57 0.04355890521169892 58-59 0.04660255684324764 60-61 0.02346494187211641 62-63 0.02475959304765479 64-65 0.025360506612149968 66-67 0.03059285155177867 68-69 0.03479924650324488 70-71 0.03909846550125914 72-73 0.043544248783296595 74-75 0.02424173257743944 76-77 0.0180225214587211 78-79 0.017436264322628247 80-81 0.0202258711952034 82-83 0.01892633454353091 84-85 0.01168117343665008 86-87 0.013024679373529533 88-89 0.01828633716996288 90-91 0.01902892954234716 92-93 0.019458851442148588 94-95 0.0227712042610732 96-97 0.023660360917480694 98-99 0.028965987999121007 100 0.028648432050404044 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.0234417E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.06575790782976 #Duplication Level Percentage of deduplicated Percentage of total 1 75.87927282151205 44.81866758697597 2 14.175352999579346 16.745559370623642 3 4.661295957726835 8.25968935727516 4 1.8967844460904146 4.481400435844535 5 0.987976372980127 2.9177786632549947 6 0.5852534546708538 2.0741063320985798 7 0.3799156453226509 1.570800387241723 8 0.2598806723168794 1.2280039100794267 9 0.19107721640539818 1.015750854531292 >10 0.9108861619035944 9.329068591066497 >50 0.04674898608654939 1.895495487675862 >100 0.022145834129825 2.4353602465271598 >500 0.0022875317797268683 0.9135510518540592 >1k 0.0010140245545784158 1.1753648754965902 >5k 4.314997651374726E-5 0.17607597690342958 >10k+ 6.472496477062088E-5 0.9633268725510498 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 33849 0.33073696332678254 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24920 0.24349213052389795 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16825 0.16439627191270398 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.9541904536428406E-5 0.0 5 0.0 0.0 0.0 1.9541904536428406E-5 0.0 6 0.0 0.0 0.0 1.9541904536428406E-5 0.0 7 0.0 0.0 0.0 1.9541904536428406E-5 0.0 8 0.0 0.0 0.0 3.908380907285681E-5 0.0 9 0.0 0.0 0.0 1.0748047495035623E-4 9.770952268214203E-6 10-11 0.0 0.0 0.0 1.3679333175499887E-4 9.770952268214203E-6 12-13 0.0 0.0 0.0 1.9053356923017696E-4 9.770952268214203E-6 14-15 0.0 0.0 0.0 2.2961737830303375E-4 1.4656428402321304E-5 16-17 0.0 0.0 0.0 2.638157112417835E-4 1.9541904536428406E-5 18-19 0.0 0.0 0.0 3.517542816557113E-4 2.931285680464261E-5 20-21 0.0 0.0 0.0 6.057990406292805E-4 3.908380907285681E-5 22-23 0.0 0.0 0.0 0.0015584668867801655 4.3969285206963913E-5 24-25 0.0 0.0 0.0 0.003957235668626753 4.8854761341071015E-5 26-27 0.0 0.0 0.0 0.007875387528180648 6.839666587749942E-5 28-29 0.0 0.0 0.0 0.019053356923017697 7.816761814571362E-5 30-31 9.770952268214203E-6 0.0 0.0 0.06279302475167857 8.793857041392783E-5 32-33 9.770952268214203E-6 0.0 0.0 0.16265704240896184 9.770952268214203E-5 34-35 1.4656428402321304E-5 0.0 0.0 0.2938907023233468 1.2702237948678465E-4 36-37 1.9541904536428406E-5 0.0 0.0 0.49557781356769026 1.2702237948678465E-4 38-39 1.9541904536428406E-5 0.0 0.0 0.8430524181299237 1.2702237948678465E-4 40-41 1.9541904536428406E-5 0.0 0.0 1.2678445679905361 1.2702237948678465E-4 42-43 1.9541904536428406E-5 0.0 0.0 1.6649800374559685 1.2702237948678465E-4 44-45 1.9541904536428406E-5 0.0 0.0 2.09201950633827 1.2702237948678465E-4 46-47 1.9541904536428406E-5 0.0 0.0 2.569828843206213 1.3190785562089175E-4 48-49 1.9541904536428406E-5 0.0 0.0 3.096546681652702 1.4656428402321306E-4 50-51 1.9541904536428406E-5 0.0 0.0 3.673135460476156 1.5633523629142725E-4 52-53 1.9541904536428406E-5 0.0 0.0 4.254516891387169 1.6122071242553437E-4 54-55 1.9541904536428406E-5 0.0 0.0 4.859548912263396 1.6610618855964146E-4 56-57 1.9541904536428406E-5 0.0 0.0 5.487381450257498 1.7587714082785568E-4 58-59 1.9541904536428406E-5 0.0 0.0 6.22208866416133 1.8564809309606987E-4 60-61 1.9541904536428406E-5 0.0 0.0 6.940937622533848 1.8564809309606987E-4 62-63 1.9541904536428406E-5 0.0 0.0 7.706736006555136 1.8564809309606987E-4 64-65 1.9541904536428406E-5 0.0 0.0 8.391865408650048 1.8564809309606987E-4 66-67 1.9541904536428406E-5 0.0 0.0 9.127852617301015 2.0030452149839118E-4 68-69 1.9541904536428406E-5 0.0 0.0 9.952306027788392 2.2961737830303378E-4 70-71 1.9541904536428406E-5 0.0 0.0 10.813107380713529 2.4427380670535506E-4 72-73 1.9541904536428406E-5 0.0 0.0 11.589179921044842 2.638157112417835E-4 74-75 1.9541904536428406E-5 0.0 0.0 12.335201897675265 2.7847213964410475E-4 76-77 1.9541904536428406E-5 0.0 0.0 13.134475564167456 2.8335761577821187E-4 78-79 1.9541904536428406E-5 9.770952268214203E-6 0.0 13.994983788524545 2.8335761577821187E-4 80-81 2.931285680464261E-5 9.770952268214203E-6 0.0 14.818538271403247 2.980140441805332E-4 82-83 2.931285680464261E-5 1.9541904536428406E-5 0.0 15.652127522261406 3.224414248510687E-4 84-85 2.931285680464261E-5 1.9541904536428406E-5 0.0 16.459076271760278 3.273269009851758E-4 86-87 2.931285680464261E-5 1.9541904536428406E-5 0.0 17.258037267779883 3.3221237711928293E-4 88 2.931285680464261E-5 1.9541904536428406E-5 0.0 17.87357306234444 3.3221237711928293E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 15470 0.0 52.231453 1 TACCTGG 9935 0.0 48.938248 2 GTATCAA 29100 0.0 42.484367 1 ACCTGGG 12020 0.0 39.81038 3 ATCAACG 35920 0.0 33.63178 3 CAACGCA 36055 0.0 33.44034 5 TATCAAC 36870 0.0 33.12685 2 GTACATG 38690 0.0 32.842823 1 TCAACGC 37545 0.0 32.175674 4 AACGCAG 38715 0.0 31.66498 6 TACATGG 39675 0.0 31.425411 2 GTACCTG 16460 0.0 30.312687 1 ACATGGG 39820 0.0 30.255222 3 CCTGGGG 11720 0.0 29.719208 4 CATGGGG 31610 0.0 28.997246 4 TGGGGGG 26620 0.0 28.517756 6 ACGCAGA 42690 0.0 28.309425 7 ATGGGGG 25160 0.0 28.192097 5 CGCAGAG 43545 0.0 27.72051 8 TAACGCA 1310 0.0 26.19378 4 >>END_MODULE