FastQCFastQC Report
Mon 6 Jun 2016
ERR1211430_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211430_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10234417
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT308210.30115051985862995No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240040.23454193824621372No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166880.1630576514519586No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA159500.050.7276341
TACCTGG108150.048.580912
ACCTGGG122650.041.918573
GTATCAA290550.041.7468071
CCTGGGG114050.033.5834584
ATCAACG368600.032.1803473
CAACGCA373300.031.7963245
TATCAAC380650.031.5439052
TCAACGC386800.030.8245744
GTACCTG175800.030.3529821
AACGCAG397800.030.2846786
TAACGCA10750.030.1650264
GTACATG376900.029.425661
TGGGGGG231700.028.9379436
CTGGGGG79150.028.9120985
ATAACGC11800.028.2769813
TACATGG384700.028.2433342
ACATGGG380500.027.3945163
ACGCAGA444800.026.7360387
CATGGGG296850.026.4388454
Produced by FastQC (version 0.11.5)