FastQCFastQC Report
Mon 6 Jun 2016
ERR1211429_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211429_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10251199
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT304040.2965896964833089No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT228160.22256908679657864No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154830.15103599100944193No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA147950.051.7423671
TACCTGG102050.050.6873662
GTATCAA273150.041.6336021
ACCTGGG122650.041.35443
CAACGCA335600.033.1126065
ATCAACG336300.033.001753
TATCAAC347300.032.3220332
GTACATG375100.032.1137921
TCAACGC351100.031.7308084
TACATGG377200.031.5317942
AACGCAG360200.031.4000516
GTACCTG167800.031.3314251
CCTGGGG119400.030.7869054
ACATGGG386350.029.6268793
CATGGGG310950.028.1483364
TGGGGGG259700.027.8757926
ACGCAGA397250.027.8565527
ATGGGGG249700.027.2594995
CGCAGAG404950.027.1966518
TAACGCA12350.025.8824624
Produced by FastQC (version 0.11.5)