##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211429_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10251199 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.199580166183488 31.0 31.0 34.0 28.0 34.0 2 31.248247741556867 31.0 31.0 34.0 28.0 34.0 3 31.307217916655407 31.0 31.0 34.0 28.0 34.0 4 34.93583638362693 37.0 35.0 37.0 32.0 37.0 5 34.98790970695233 37.0 35.0 37.0 32.0 37.0 6 35.048119346819824 37.0 35.0 37.0 32.0 37.0 7 34.972296118727186 37.0 35.0 37.0 32.0 37.0 8 34.96984343002219 37.0 35.0 37.0 32.0 37.0 9 36.51541327019405 39.0 37.0 39.0 32.0 39.0 10-11 36.431111619235956 39.0 37.0 39.0 32.0 39.0 12-13 36.32951047970096 39.0 37.0 39.0 32.0 39.0 14-15 37.489680963173186 40.0 37.0 41.0 32.0 41.0 16-17 37.47299125692517 40.0 37.0 41.0 32.0 41.0 18-19 37.46349378253217 40.0 37.0 41.0 31.0 41.0 20-21 37.447740454555614 40.0 37.0 41.0 31.0 41.0 22-23 37.32935766830788 40.0 37.0 41.0 31.0 41.0 24-25 37.23922089503871 40.0 36.0 41.0 31.0 41.0 26-27 37.10573870432132 40.0 36.0 41.0 30.5 41.0 28-29 36.964613602760025 39.5 36.0 41.0 30.0 41.0 30-31 36.805165181165634 39.0 36.0 41.0 30.0 41.0 32-33 36.605728900590066 39.0 35.0 41.0 30.0 41.0 34-35 36.49227183083657 39.0 35.0 41.0 30.0 41.0 36-37 36.37945019894746 39.0 35.0 41.0 29.0 41.0 38-39 36.230631363219075 39.0 35.0 40.0 29.0 41.0 40-41 36.06433974211212 39.0 35.0 40.0 28.0 41.0 42-43 35.879525946184444 38.0 35.0 40.0 27.0 41.0 44-45 35.76076652106744 38.0 35.0 40.0 27.0 41.0 46-47 35.54683491170155 38.0 34.0 40.0 26.0 41.0 48-49 35.31357653870538 38.0 34.0 40.0 25.5 41.0 50-51 34.66025749768393 37.5 33.5 39.5 25.0 40.5 52-53 34.97385344875268 38.0 33.5 39.5 26.0 40.5 54-55 35.39272840181914 38.0 34.0 40.0 26.0 41.0 56-57 35.323584782619086 38.0 34.0 40.0 26.0 41.0 58-59 35.12766770013927 38.0 34.0 40.0 26.0 41.0 60-61 34.90686996711312 38.0 34.0 40.0 26.0 41.0 62-63 34.61771983940611 37.0 33.0 40.0 25.5 41.0 64-65 34.41639670637552 37.0 33.0 40.0 25.0 41.0 66-67 34.23538827019161 36.0 33.0 39.5 25.0 41.0 68-69 33.92142348421878 36.0 33.0 39.0 24.5 41.0 70-71 33.576758386994534 35.0 32.5 39.0 24.0 41.0 72-73 33.22124231516723 35.0 32.0 38.5 23.5 40.0 74-75 32.87147820464709 35.0 32.0 37.0 23.0 39.5 76-77 32.520837806387334 35.0 31.5 37.0 22.5 39.0 78-79 32.170835528605 35.0 31.0 36.0 22.0 39.0 80-81 31.83222250392369 35.0 31.0 36.0 20.5 38.0 82-83 31.508170897862776 35.0 31.0 35.5 20.0 37.0 84-85 31.210806072538443 34.0 30.5 35.0 20.0 37.0 86-87 30.923790475631193 34.0 30.0 35.0 19.0 36.0 88-89 30.671868822368975 34.0 30.0 35.0 18.5 36.0 90-91 30.427257045736795 34.0 29.5 35.0 18.0 36.0 92-93 30.188776795767986 34.0 29.0 35.0 17.5 35.0 94-95 29.955967979940688 34.0 29.0 35.0 16.5 35.0 96-97 29.70987179158262 34.0 29.0 35.0 15.0 35.0 98-99 29.447943211325818 34.0 28.5 35.0 12.0 35.0 100 28.22674674445399 33.0 25.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3703716447289942 1101 2 -0.29572761440701 1101 3 -0.33017515384492313 1101 4 -0.21143306351678604 1101 5 -0.22295091032810888 1101 6 -0.17986865013968156 1101 7 -0.282232771776016 1101 8 -0.2456143404389053 1101 9 -0.3967769685790046 1101 10-11 -0.4268798774938034 1101 12-13 -0.40522849406135464 1101 14-15 -0.6490158896471954 1101 16-17 -0.595010634070924 1101 18-19 -0.624239180019373 1101 20-21 -0.5852558825268162 1101 22-23 -0.6558094887602692 1101 24-25 -0.7053306568840796 1101 26-27 -0.7332257726755813 1101 28-29 -0.7720498203105635 1101 30-31 -0.8079372228220407 1101 32-33 -0.769522192860201 1101 34-35 -0.8015108563440663 1101 36-37 -0.8517666923092051 1101 38-39 -0.8668583940773615 1101 40-41 -1.0359042457500678 1101 42-43 -1.0605054233295022 1101 44-45 -1.0262817104508812 1101 46-47 -1.0472972815902537 1101 48-49 -1.0008852340239898 1101 50-51 -0.985728009935535 1101 52-53 -0.7531621058106097 1101 54-55 -0.708324311076602 1101 56-57 -0.8220149803810202 1101 58-59 -0.7624189297105914 1101 60-61 -0.7530316431248991 1101 62-63 -0.8585101024240132 1101 64-65 -0.8170717807537997 1101 66-67 -0.9267878485920704 1101 68-69 -0.938649672697764 1101 70-71 -0.909611143992926 1101 72-73 -1.0396781123711918 1101 74-75 -1.040205215689138 1101 76-77 -0.9841304474543691 1101 78-79 -1.042504804028951 1101 80-81 -1.1613643250686323 1101 82-83 -1.157345092081414 1101 84-85 -1.1453705388766515 1101 86-87 -1.2971548440012448 1101 88-89 -1.3598674714218113 1101 90-91 -1.420661253970163 1101 92-93 -1.5793886370427934 1101 94-95 -1.6359287828925915 1101 96-97 -1.7114275236411203 1101 98-99 -1.8696997935167659 1101 100 -1.9591046953390325 1102 1 -0.38111553265682474 1102 2 -0.38245621823998377 1102 3 -0.4820291404617194 1102 4 -0.4244892200368753 1102 5 -0.30048238429974106 1102 6 -0.3919303714165565 1102 7 -0.49796151600396144 1102 8 -0.3961328609748236 1102 9 -0.5576491694906665 1102 10-11 -0.5115546831204512 1102 12-13 -0.5906570022060862 1102 14-15 -0.7330877442186221 1102 16-17 -0.7891730805912474 1102 18-19 -0.7789242320773155 1102 20-21 -0.8655523353374264 1102 22-23 -0.8323794320503595 1102 24-25 -0.8499412795533985 1102 26-27 -0.9370180528454668 1102 28-29 -0.9236538544426978 1102 30-31 -0.8567277461310638 1102 32-33 -0.8299488210274149 1102 34-35 -0.9206175931552139 1102 36-37 -0.9563034167636815 1102 38-39 -1.0265660698722812 1102 40-41 -1.216323724289893 1102 42-43 -1.1826747338838572 1102 44-45 -1.2405561098951168 1102 46-47 -1.268526035599912 1102 48-49 -1.3280942087813585 1102 50-51 -1.1104337337017043 1102 52-53 -0.9386459581619206 1102 54-55 -1.018145341646587 1102 56-57 -0.9198989394707482 1102 58-59 -0.9273342073721338 1102 60-61 -1.034668417896313 1102 62-63 -0.9388763318391895 1102 64-65 -1.026959484362358 1102 66-67 -1.0281731827527452 1102 68-69 -1.0551572429387974 1102 70-71 -1.0426648831825318 1102 72-73 -1.0427383776768835 1102 74-75 -1.1424681641640433 1102 76-77 -1.0859608145581916 1102 78-79 -1.186882898253355 1102 80-81 -1.2698064186479812 1102 82-83 -1.3150529148235393 1102 84-85 -1.3744047963459245 1102 86-87 -1.348760320323894 1102 88-89 -1.4742301126390203 1102 90-91 -1.484439896637351 1102 92-93 -1.509734134823649 1102 94-95 -1.6272018106352775 1102 96-97 -1.681065303678782 1102 98-99 -1.754337499754378 1102 100 -1.7299354117630834 1103 1 -0.38745400528797447 1103 2 -0.4040945217314835 1103 3 -0.4848742044373431 1103 4 -0.2426195765392336 1103 5 -0.2588240378752644 1103 6 -0.3167578225213319 1103 7 -0.37658487796741724 1103 8 -0.31755342498010464 1103 9 -0.41302070206565844 1103 10-11 -0.4122228163475583 1103 12-13 -0.5864081578405305 1103 14-15 -0.6382288875776609 1103 16-17 -0.7059479420681996 1103 18-19 -0.7441170753686634 1103 20-21 -0.6705911948091199 1103 22-23 -0.6753920834050646 1103 24-25 -0.6547194462215913 1103 26-27 -0.789323003581778 1103 28-29 -0.743727649228461 1103 30-31 -0.7833412518618772 1103 32-33 -0.7722311046591983 1103 34-35 -0.7422620388471657 1103 36-37 -0.8191064916563136 1103 38-39 -0.8450110709388667 1103 40-41 -0.866669891763145 1103 42-43 -0.8756781736914618 1103 44-45 -0.9005133889854235 1103 46-47 -0.9902349510155517 1103 48-49 -0.9467908004107315 1103 50-51 -0.9092004174945956 1103 52-53 -0.7332827954164216 1103 54-55 -0.7306721057823538 1103 56-57 -0.715077404471721 1103 58-59 -0.7378113224907068 1103 60-61 -0.7244804678484655 1103 62-63 -0.7520521865101912 1103 64-65 -0.6910078734452014 1103 66-67 -0.7822524940855828 1103 68-69 -0.691047617730753 1103 70-71 -0.7689401517059409 1103 72-73 -0.7770795688688636 1103 74-75 -0.8767064729139449 1103 76-77 -0.9306103611640779 1103 78-79 -0.8634282862329741 1103 80-81 -0.8449329419566567 1103 82-83 -0.9064410073507965 1103 84-85 -1.0097680221033407 1103 86-87 -1.1132103232739965 1103 88-89 -1.1067405002234416 1103 90-91 -1.1174551218943698 1103 92-93 -1.186057987404432 1103 94-95 -1.226466556274005 1103 96-97 -1.3034493549444335 1103 98-99 -1.3877847950275282 1103 100 -1.3581772564517571 1104 1 -0.5264944057196104 1104 2 -0.5014964793543868 1104 3 -0.4663534166856529 1104 4 -0.311373852163193 1104 5 -0.40488467794016003 1104 6 -0.29031883673069103 1104 7 -0.36936722413202716 1104 8 -0.5088923665134288 1104 9 -0.4928694152574522 1104 10-11 -0.5468905032970994 1104 12-13 -0.633754081971297 1104 14-15 -0.8569796722766299 1104 16-17 -0.8014792123820484 1104 18-19 -0.802646321927071 1104 20-21 -0.7846313050116365 1104 22-23 -0.8197295566476299 1104 24-25 -0.8206722095801169 1104 26-27 -0.8072727757466609 1104 28-29 -1.0325821056400528 1104 30-31 -0.9701841588126925 1104 32-33 -0.9426426633419069 1104 34-35 -0.9256717279404114 1104 36-37 -1.0618455954329775 1104 38-39 -1.0602087386973196 1104 40-41 -1.0484664266399406 1104 42-43 -1.0105928240209607 1104 44-45 -1.033397194580921 1104 46-47 -1.1478836459083155 1104 48-49 -1.1252983560501093 1104 50-51 -1.0408190635235144 1104 52-53 -0.8469919743394101 1104 54-55 -0.8164370608804674 1104 56-57 -0.7773024182141057 1104 58-59 -0.8131188783543237 1104 60-61 -0.8378928947892703 1104 62-63 -0.8819577332220945 1104 64-65 -0.9203835026496847 1104 66-67 -0.876219505323327 1104 68-69 -0.937237002735344 1104 70-71 -0.8461543377412397 1104 72-73 -0.8776260744015687 1104 74-75 -0.9394968086133879 1104 76-77 -0.9943884335909239 1104 78-79 -0.9752077042484792 1104 80-81 -0.962023915041307 1104 82-83 -0.937426791542773 1104 84-85 -1.0113409646947567 1104 86-87 -1.1348574276410694 1104 88-89 -1.1029836914890154 1104 90-91 -1.121629556910527 1104 92-93 -1.1195355095264894 1104 94-95 -1.0972100366358148 1104 96-97 -1.135340060705289 1104 98-99 -1.3017838258374326 1104 100 -1.219446869779901 1105 1 -0.47240247161779436 1105 2 -0.4574819853561216 1105 3 -0.5282173968669781 1105 4 -0.34887692004362947 1105 5 -0.3868269327872724 1105 6 -0.3917662298370246 1105 7 -0.4547559157732195 1105 8 -0.44930489166308973 1105 9 -0.47036485400891337 1105 10-11 -0.5217182048018003 1105 12-13 -0.5765772417334887 1105 14-15 -0.8070139728297505 1105 16-17 -0.7079188701872923 1105 18-19 -0.6888867114983981 1105 20-21 -0.7927070078430063 1105 22-23 -0.7989781933908802 1105 24-25 -0.7780654725932337 1105 26-27 -0.7929474407579065 1105 28-29 -0.7686690237489344 1105 30-31 -0.7340030887045756 1105 32-33 -0.845496902130229 1105 34-35 -0.8444070711935296 1105 36-37 -0.7903911392993237 1105 38-39 -0.8686521360058279 1105 40-41 -0.8229619395996437 1105 42-43 -0.8045596191282058 1105 44-45 -0.8537666502133376 1105 46-47 -0.950866305823844 1105 48-49 -1.0134038283766529 1105 50-51 -1.0173178760343689 1105 52-53 -0.8697066239505418 1105 54-55 -0.7453666560154275 1105 56-57 -0.7427111151068928 1105 58-59 -0.7753350267431713 1105 60-61 -0.7192454274836564 1105 62-63 -0.8038367251984013 1105 64-65 -0.8118914210471857 1105 66-67 -0.7858321158057393 1105 68-69 -0.7772035533549158 1105 70-71 -0.7551088718766081 1105 72-73 -0.7879707450168212 1105 74-75 -0.8065021433920379 1105 76-77 -0.8930462249567412 1105 78-79 -0.951377843342037 1105 80-81 -0.9076547662081538 1105 82-83 -0.992725204559143 1105 84-85 -0.8913006785481024 1105 86-87 -0.9445382913083868 1105 88-89 -0.8839438183137425 1105 90-91 -0.957768030225477 1105 92-93 -1.0048008439792149 1105 94-95 -1.0306258752085817 1105 96-97 -1.0399636477687366 1105 98-99 -1.0387478872707305 1105 100 -1.0322005970161392 1106 1 -0.4694413863829894 1106 2 -0.5185437352340081 1106 3 -0.5309958142837665 1106 4 -0.3595396878197832 1106 5 -0.35234104991099 1106 6 -0.42754315137076304 1106 7 -0.43069862737743136 1106 8 -0.4507674335303733 1106 9 -0.6478866162725367 1106 10-11 -0.7230285961736556 1106 12-13 -0.5985483511292031 1106 14-15 -0.7990803629979126 1106 16-17 -0.7807998951506576 1106 18-19 -0.80996839194637 1106 20-21 -0.8398407999719595 1106 22-23 -0.7222661814063684 1106 24-25 -0.7823124939106592 1106 26-27 -0.8448911946829654 1106 28-29 -0.830948511834201 1106 30-31 -0.833009514896041 1106 32-33 -0.9474097571298827 1106 34-35 -0.8808799272398602 1106 36-37 -0.9563675300780545 1106 38-39 -0.989913093458199 1106 40-41 -0.9659450705744632 1106 42-43 -0.9325583304009726 1106 44-45 -0.9959400854237188 1106 46-47 -0.9427113118422596 1106 48-49 -1.024129200821001 1106 50-51 -0.975710641281033 1106 52-53 -0.911452554974197 1106 54-55 -0.9122576833338272 1106 56-57 -0.9336661906424482 1106 58-59 -0.8721291051396562 1106 60-61 -0.7633215016410801 1106 62-63 -0.8285422259485173 1106 64-65 -0.8348644340958202 1106 66-67 -0.8703264375557964 1106 68-69 -0.8863095887851031 1106 70-71 -0.8736367681329753 1106 72-73 -0.8167838948319073 1106 74-75 -0.8795251791187866 1106 76-77 -0.9593677700338432 1106 78-79 -0.9163568660899273 1106 80-81 -0.9561671257783146 1106 82-83 -0.8484236685532771 1106 84-85 -0.9192471320405282 1106 86-87 -0.8874823025427752 1106 88-89 -0.9820705409778974 1106 90-91 -0.9463311974852608 1106 92-93 -0.9194176602341919 1106 94-95 -0.9700139696367458 1106 96-97 -0.978111445811443 1106 98-99 -1.041340922038021 1106 100 -1.1390098523018821 1107 1 -0.502623700513201 1107 2 -0.5364274767620429 1107 3 -0.5106256150642139 1107 4 -0.4075594300666481 1107 5 -0.42038023916956746 1107 6 -0.42892311902325986 1107 7 -0.43567603546944156 1107 8 -0.5108330912629668 1107 9 -0.5087542225571937 1107 10-11 -0.5208780968411375 1107 12-13 -0.6419593120554694 1107 14-15 -0.7887195984672104 1107 16-17 -0.8877434089625282 1107 18-19 -0.9086481131416946 1107 20-21 -0.8756231731492079 1107 22-23 -0.8059363716405059 1107 24-25 -0.7736585737011765 1107 26-27 -0.8161185385934218 1107 28-29 -0.8614532035060094 1107 30-31 -0.8967517810184518 1107 32-33 -0.9086467924554071 1107 34-35 -0.889750845292852 1107 36-37 -0.8870735592934977 1107 38-39 -0.9445836174364288 1107 40-41 -0.9023470525009358 1107 42-43 -0.9353000702346179 1107 44-45 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58-59 1.2943380807857707 2311 60-61 1.251507237441885 2311 62-63 1.198011159030763 2311 64-65 1.1682400614813844 2311 66-67 1.1885010313726596 2311 68-69 1.2114543619203104 2311 70-71 1.2264603129609668 2311 72-73 1.2447345521505113 2311 74-75 1.2019598869432429 2311 76-77 1.1977553474781928 2311 78-79 1.2063185761897728 2311 80-81 1.1858780679289964 2311 82-83 1.2193825331598482 2311 84-85 1.241947093130218 2311 86-87 1.3003976241171706 2311 88-89 1.3295481537371394 2311 90-91 1.3440588764146355 2311 92-93 1.3994749434776033 2311 94-95 1.5238517263939357 2311 96-97 1.536038967776065 2311 98-99 1.6370410655613554 2311 100 1.7566620699352704 2312 1 0.5755020384087111 2312 2 0.6125262276584493 2312 3 0.7062980940095613 2312 4 0.5381548508879419 2312 5 0.5596745000807317 2312 6 0.45436680076188196 2312 7 0.5781164142823343 2312 8 0.6317820719322995 2312 9 0.8185303331839577 2312 10-11 0.8643742465538224 2312 12-13 0.882874728314043 2312 14-15 1.0910254935103936 2312 16-17 1.098003308996013 2312 18-19 1.0519025213957889 2312 20-21 1.0875252197267642 2312 22-23 1.1335489516427941 2312 24-25 1.1244986887025945 2312 26-27 1.140556630594304 2312 28-29 1.1695944360083175 2312 30-31 1.1144110884119058 2312 32-33 1.1977336035168094 2312 34-35 1.2190264072522154 2312 36-37 1.251275528811199 2312 38-39 1.3033555861892125 2312 40-41 1.3148175653026044 2312 42-43 1.3652867852669601 2312 44-45 1.3954044767348606 2312 46-47 1.3621845818425626 2312 48-49 1.3155027758109057 2312 50-51 1.313227129438495 2312 52-53 1.1717282615296938 2312 54-55 1.1737369264162183 2312 56-57 1.0919323409196622 2312 58-59 1.0977169985683375 2312 60-61 1.0948988106002702 2312 62-63 1.134908197891349 2312 64-65 1.1023819517122675 2312 66-67 1.1089848312738724 2312 68-69 1.1529880492301245 2312 70-71 1.179314221286539 2312 72-73 1.1849487791183222 2312 74-75 1.222073514530173 2312 76-77 1.1722389762278098 2312 78-79 1.1613358103433384 2312 80-81 1.0349966233250285 2312 82-83 1.1885795697350865 2312 84-85 1.2439996055305151 2312 86-87 1.3536980001113115 2312 88-89 1.3511115515288594 2312 90-91 1.383553962256503 2312 92-93 1.471812604829541 2312 94-95 1.5028684604934774 2312 96-97 1.5579123137068507 2312 98-99 1.4999805082577353 2312 100 1.6305055942301472 2313 1 0.6225154984612757 2313 2 0.6540367335373176 2313 3 0.6705206462845723 2313 4 0.5379297898369799 2313 5 0.5701546762785128 2313 6 0.6225631649969898 2313 7 0.619941642324747 2313 8 0.6526268165310469 2313 9 0.8423412691040397 2313 10-11 0.7285463200562461 2313 12-13 0.8627709554045211 2313 14-15 1.0951522363660473 2313 16-17 1.0760608630796256 2313 18-19 1.027607077916862 2313 20-21 1.1082018971863405 2313 22-23 1.1049139353940518 2313 24-25 1.1499624955114385 2313 26-27 1.207290726330875 2313 28-29 1.2055517278745285 2313 30-31 1.2165623064467397 2313 32-33 1.2637950138416514 2313 34-35 1.2647408927857953 2313 36-37 1.2791425760762323 2313 38-39 1.3278145317363013 2313 40-41 1.3270946408878856 2313 42-43 1.378905168186364 2313 44-45 1.3264321609115441 2313 46-47 1.3186360005195397 2313 48-49 1.338657178604656 2313 50-51 1.2960098404226201 2313 52-53 1.1469741453369906 2313 54-55 1.1086970142748882 2313 56-57 1.167115729006504 2313 58-59 1.1572045642977358 2313 60-61 1.0912786923438489 2313 62-63 1.1023372363873492 2313 64-65 1.0815764457120096 2313 66-67 1.0712478090251594 2313 68-69 1.163186139963571 2313 70-71 1.1133725839198192 2313 72-73 1.0927065577654744 2313 74-75 1.0475884718243478 2313 76-77 1.050524941114574 2313 78-79 1.085920264502711 2313 80-81 1.1780743197762433 2313 82-83 1.145311667414294 2313 84-85 1.2598544123320288 2313 86-87 1.2612419863497486 2313 88-89 1.2937204040034018 2313 90-91 1.2975266970360693 2313 92-93 1.4004101461493974 2313 94-95 1.4522557351035168 2313 96-97 1.5230301350377928 2313 98-99 1.561257175135296 2313 100 1.745554961185956 2314 1 0.4941375063894746 2314 2 0.5048533449311456 2314 3 0.5351939903111145 2314 4 0.45761513394458575 2314 5 0.4897049040175503 2314 6 0.5048928973987543 2314 7 0.5379658317837155 2314 8 0.5576070605829457 2314 9 0.715656992181394 2314 10-11 0.6778912872169158 2314 12-13 0.4317570076926458 2314 14-15 0.8130647753799778 2314 16-17 0.8307817288988915 2314 18-19 0.8558911299651584 2314 20-21 0.9234583476856315 2314 22-23 0.7862904191373303 2314 24-25 0.8270015697390818 2314 26-27 0.9235864185538887 2314 28-29 0.9739539261308039 2314 30-31 0.843874670268697 2314 32-33 0.9143002931521877 2314 34-35 0.925161483928477 2314 36-37 1.0427431952305923 2314 38-39 1.0627292829828079 2314 40-41 1.0916721520565602 2314 42-43 1.1593640517632053 2314 44-45 1.1435200977174773 2314 46-47 1.1800676029030583 2314 48-49 1.201300981707 2314 50-51 1.0963520350128064 2314 52-53 0.9628508288455961 2314 54-55 0.9584803235748467 2314 56-57 0.9427581358639756 2314 58-59 0.9634321480285237 2314 60-61 1.0097817682264676 2314 62-63 0.9851899019437198 2314 64-65 0.9211736044946477 2314 66-67 0.938025342756525 2314 68-69 0.9411745064442343 2314 70-71 0.962595053161273 2314 72-73 0.9722600304399478 2314 74-75 0.9987599672306544 2314 76-77 1.0349589663327663 2314 78-79 1.0131093979113857 2314 80-81 1.0414040560547306 2314 82-83 0.9583773646819402 2314 84-85 0.9390893183723499 2314 86-87 1.175565945760713 2314 88-89 1.273481769748944 2314 90-91 1.29109613879141 2314 92-93 1.3547613086767747 2314 94-95 1.4018852132796127 2314 96-97 1.4361601156921147 2314 98-99 1.4932424240702318 2314 100 1.4301608767881362 2315 1 0.39628239394880893 2315 2 0.3902171636779528 2315 3 0.44422213029084645 2315 4 0.4432277605387327 2315 5 0.3544445958432334 2315 6 -0.31413133745516575 2315 7 0.19888945266350078 2315 8 0.27217524745048394 2315 9 0.49407870583029023 2315 10-11 0.5785130191545065 2315 12-13 0.578168690392701 2315 14-15 0.6590120244276889 2315 16-17 0.6271414602868504 2315 18-19 0.7161108023252609 2315 20-21 0.7554899041566614 2315 22-23 0.6200509567329249 2315 24-25 0.7762636032184247 2315 26-27 0.821365597148656 2315 28-29 0.9162058832467253 2315 30-31 0.8967560070061396 2315 32-33 0.9445003213759477 2315 34-35 0.9371142689843168 2315 36-37 0.9844042012582932 2315 38-39 1.0343517602018153 2315 40-41 1.029667524023047 2315 42-43 1.0749162065425537 2315 44-45 1.0906449109405045 2315 46-47 1.174854018508391 2315 48-49 1.1095887252380905 2315 50-51 1.0827052165937872 2315 52-53 0.9321319414708213 2315 54-55 0.7796396615397327 2315 56-57 0.7197353387400156 2315 58-59 0.7734441607971618 2315 60-61 0.6869684641220317 2315 62-63 0.8516843241376577 2315 64-65 0.9179290874899166 2315 66-67 0.9292315631713635 2315 68-69 0.9560905914887954 2315 70-71 1.003994073515507 2315 72-73 0.9603072486869877 2315 74-75 1.023015639200139 2315 76-77 0.953496926917488 2315 78-79 0.8533265352458841 2315 80-81 0.8553914270481613 2315 82-83 1.047960476433392 2315 84-85 1.028465039056396 2315 86-87 1.0728158479244918 2315 88-89 1.2167694865597376 2315 90-91 1.2535448831481695 2315 92-93 1.314852520847655 2315 94-95 1.4205170766924695 2315 96-97 1.503626417261195 2315 98-99 1.5278222645513537 2315 100 1.6502446368715837 2316 1 0.05761003820494892 2316 2 -0.033621421478553515 2316 3 0.09423673018767076 2316 4 0.04305985048704031 2316 5 0.026272376740521963 2316 6 0.1013509808158446 2316 7 0.1188234842648086 2316 8 0.1370220471137813 2316 9 0.34100334467618865 2316 10-11 0.13529759871050118 2316 12-13 0.21698544895830452 2316 14-15 0.45248530420423805 2316 16-17 0.4710730110675101 2316 18-19 0.4222988431841017 2316 20-21 0.3668646404323965 2316 22-23 0.27879793729513835 2316 24-25 0.31135542820882023 2316 26-27 0.44233083435080545 2316 28-29 0.5301148803699078 2316 30-31 0.5651307456308174 2316 32-33 0.6417298903637842 2316 34-35 0.704037725504179 2316 36-37 0.7433089622618851 2316 38-39 0.8108616180651893 2316 40-41 0.8101725599665102 2316 42-43 0.8199792719616994 2316 44-45 0.7399240709903481 2316 46-47 0.7007120949244481 2316 48-49 0.837806914752484 2316 50-51 0.6433061342786104 2316 52-53 0.38172551198763216 2316 54-55 0.401228206290817 2316 56-57 0.5282953115256248 2316 58-59 0.5893739363539581 2316 60-61 0.6813034420349595 2316 62-63 0.6984857507317273 2316 64-65 0.6789515220727154 2316 66-67 0.6758666017828219 2316 68-69 0.6417013062270414 2316 70-71 0.49119176623606364 2316 72-73 0.6106525978672082 2316 74-75 0.715593362400945 2316 76-77 0.8034710634562217 2316 78-79 0.8059761374625651 2316 80-81 0.8273467217519226 2316 82-83 0.9005369585527454 2316 84-85 0.9552825710914128 2316 86-87 1.0612889699541554 2316 88-89 1.070966291624039 2316 90-91 1.129461158129498 2316 92-93 1.1945798345742098 2316 94-95 1.2525064253492815 2316 96-97 1.2895297681412288 2316 98-99 1.4246549059078042 2316 100 1.3542777280209748 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 45.0 1 566.0 2 290.0 3 199.0 4 187.0 5 135.0 6 198.0 7 1324.0 8 4558.0 9 8014.0 10 11123.0 11 15011.0 12 19549.0 13 23084.0 14 26862.0 15 31551.0 16 38040.0 17 47131.0 18 57185.0 19 67449.0 20 80356.0 21 94221.0 22 104863.0 23 108808.0 24 110216.0 25 116114.0 26 127860.0 27 148211.0 28 174751.0 29 206321.0 30 247816.0 31 306648.0 32 383881.0 33 497458.0 34 691088.0 35 918051.0 36 1327554.0 37 1936100.0 38 1836666.0 39 481206.0 40 509.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.01201273863556 18.718100251986442 12.396549912134699 23.8733370972433 2 16.0553683044993 20.18979086682254 40.85751724605512 22.897323582623045 3 17.986387171570854 27.544267841639392 30.303266840747785 24.16607814604197 4 12.344202074498876 16.836611186745003 38.42024335606448 32.39894338269164 5 12.643136670211277 37.873518479235145 34.22824508982944 15.25509976072414 6 29.331740063094436 36.946349816321046 19.883620217627207 13.83828990295731 7 26.92813609086259 33.16528581970382 23.14294303908669 16.763635050346902 8 26.45281000898037 35.15479061953825 20.664350308066453 17.72804906341493 9 28.357598944387608 15.352578813017697 20.32233418298511 35.96748805960958 10-11 25.071631511082217 25.67550324067353 29.178348665486613 20.07451658275764 12-13 25.357528200768154 24.01736469350097 28.40187319384117 22.223233911889704 14-15 23.13657311870321 25.240899812022043 26.50190641613086 25.12062065314388 16-17 22.484230783528265 27.393218678339203 27.249212892935194 22.873337645197335 18-19 22.03558299495362 27.18606399206036 28.880676539568327 21.897676473417686 20-21 23.136979331749146 26.692772027422627 28.21288419857956 21.957364442248668 22-23 22.865130271647224 26.469111996801914 28.207745567380126 22.45801216417074 24-25 22.201528480242125 26.140990113268803 28.748406481315115 22.909074925173957 26-27 22.2555771160375 26.36543286678653 28.38653616417431 22.992453853001656 28-29 22.262297223666366 27.465670795861296 28.158930973955226 22.11310100651711 30-31 23.385567302606187 26.51765900898767 28.34984409130714 21.746929597098998 32-33 23.252784946520887 26.485710558369007 28.043892125163474 22.21761236994663 34-35 23.034915163905996 26.781434053888916 28.297100646756356 21.886550135448736 36-37 22.657161245577235 26.78707439144834 28.202413033173645 22.35335132980078 38-39 22.830286536763314 27.014859118721375 28.110672594696105 22.04418174981921 40-41 22.857454116450693 26.250058605053844 28.566052274293213 22.326435004202246 42-43 22.544499613267455 26.620084419081756 27.868728244922185 22.966687722728597 44-45 21.885474602734213 27.03854853862656 28.483761319170377 22.592215539468846 46-47 21.83748671879247 26.371527745585592 28.18298344400559 23.60800209161635 48-49 22.262510837280168 26.433487693416907 28.24083471552164 23.06316675378129 50-51 22.203131158825595 26.901416002663087 27.995563730609437 22.89988910790188 52-53 21.894576883024627 27.109412157544 27.72548370331344 23.270527256117933 54-55 21.71585444128457 26.6846356977225 28.28848834021721 23.31102152077572 56-57 22.26086536446331 26.538432354318246 27.849710257760922 23.350992023457522 58-59 21.781458325159655 26.70583494415839 28.38239591220325 23.130310818478705 60-61 22.433007471268322 26.87012835773725 27.871229818645098 22.82563435234933 62-63 22.480263514813632 26.608776749684736 27.785642860850707 23.125316874650924 64-65 21.999436696486885 26.203541669604096 27.973288504534555 23.823733129374464 66-67 22.03252936134984 26.0615651326402 27.844694396740955 24.061211109269003 68-69 21.490895212608006 26.32118538717803 28.206226657159327 23.981692743054637 70-71 21.840751573573137 26.04487076175056 28.352119305868793 23.762258358807514 72-73 21.88608404334971 26.622120344555405 27.82326174484069 23.668533867254197 74-75 22.16972131772256 26.470120596395226 27.629000098696345 23.731157987185867 76-77 21.986754365972036 26.18374841354462 27.808030898608916 24.021466321874428 78-79 21.745547603335805 26.210562153785617 28.14157439120833 23.90231585167025 80-81 22.017727035807862 26.26611160644505 27.891956641920785 23.8242047158263 82-83 22.566319292383465 26.14335612839353 28.042524392213714 23.24780018700929 84-85 22.649656535580103 26.409321198013263 27.459782809120416 23.481239457286215 86-87 22.174574263546106 26.687343113205714 27.748717124689033 23.389365498559147 88-89 22.31129736603783 25.94165694077454 27.798129896810252 23.94891579637738 90-91 23.002314088130486 25.590975470958522 27.96218525828048 23.444525182630507 92-93 23.037971225397637 25.89873738217612 27.689726238073085 23.373565154353155 94-95 22.72770958691909 25.72539701605081 27.807954849593123 23.738938547436973 96-97 22.767736003646124 25.623531092324313 27.7841785977072 23.824554306322362 98-99 22.727804307429718 25.734085473644996 27.5430463165813 23.995063902343993 100 22.93169991117516 25.67315092251279 27.465611476056566 23.929537690255486 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1136.0 1 876.5 2 1822.5 3 2819.0 4 2037.5 5 1558.5 6 2072.5 7 3610.0 8 5867.0 9 7048.0 10 6520.5 11 6136.0 12 6713.5 13 7577.0 14 8456.5 15 9509.5 16 10614.5 17 11457.5 18 12414.5 19 13412.0 20 14444.0 21 16006.5 22 18242.0 23 21656.5 24 26375.0 25 32257.5 26 40342.5 27 49974.5 28 62389.5 29 79367.5 30 99568.0 31 119124.0 32 144147.5 33 169408.5 34 191180.5 35 213470.5 36 238345.5 37 271056.5 38 288457.5 39 288317.0 40 289100.0 41 293345.5 42 301772.5 43 315942.5 44 334278.5 45 360329.5 46 387798.0 47 412753.5 48 440802.0 49 462536.5 50 468263.5 51 460430.0 52 439194.5 53 412125.5 54 385258.5 55 348132.5 56 310966.5 57 271086.0 58 227241.5 59 189631.0 60 151212.0 61 116817.5 62 91658.5 63 71655.5 64 53193.5 65 37711.0 66 27619.0 67 20897.5 68 15966.5 69 12150.5 70 9266.5 71 6992.0 72 5265.5 73 3933.5 74 2910.5 75 2113.5 76 1506.0 77 1051.0 78 748.5 79 546.0 80 395.5 81 306.5 82 226.5 83 173.5 84 136.0 85 98.5 86 80.5 87 74.0 88 63.5 89 49.5 90 39.5 91 31.5 92 25.0 93 20.0 94 16.0 95 13.5 96 11.0 97 7.0 98 3.5 99 1.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2192328916841825 2 0.1472022931171271 3 0.02279733326804016 4 0.02210473135874155 5 0.027089514114397743 6 0.054676531008714196 7 0.061251371668816494 8 0.053847359708849664 9 0.04151709473204061 10-11 0.04376561219814385 12-13 0.04209263716371128 14-15 0.02072928249661332 16-17 0.01910020476629124 18-19 0.018192993814674752 20-21 0.02164137092646431 22-23 0.025128767864129844 24-25 0.02205107909816208 26-27 0.01946113815564404 28-29 0.018968512853959812 30-31 0.01727115042835477 32-33 0.025538476035827613 34-35 0.026065243685153318 36-37 0.024592245258335147 38-39 0.02316314413562745 40-41 0.024480063258941708 42-43 0.03083053992025713 44-45 0.03991728187112551 46-47 0.04465819071505685 48-49 0.04060988378042413 50-51 0.028762489148830298 52-53 0.04389242663224077 54-55 0.04204386238136632 56-57 0.04478988262738827 58-59 0.04777002182866609 60-61 0.024753202040073558 62-63 0.026016468902808346 64-65 0.026606643769182512 66-67 0.032074296870053934 68-69 0.035878729892961786 70-71 0.039590490829414196 72-73 0.043770489676378345 74-75 0.025289724645868254 76-77 0.018875840767504367 78-79 0.018139341554095283 80-81 0.021665758317636796 82-83 0.019270916504498643 84-85 0.011988841500394247 86-87 0.01338380027546046 88-89 0.01877829120281442 90-91 0.019719644502072392 92-93 0.0200610679784872 94-95 0.023724054132594635 96-97 0.024504450650114196 98-99 0.029484355927535892 100 0.028679572018843847 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.0251199E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.716303287123814 #Duplication Level Percentage of deduplicated Percentage of total 1 76.12451043121705 45.45874352494377 2 14.19288858401963 16.950936784073473 3 4.462708282452756 7.994893238307248 4 1.90093772924546 4.540678958782333 5 0.9877847020993248 2.9493425426472264 6 0.595045457648983 2.1320349011155235 7 0.3508224175867708 1.4664872521973524 8 0.2636575458959804 1.2595723179730507 9 0.18947761791984025 1.0183412608040652 >10 0.8618662333229302 8.928054638016198 >50 0.0446522427197437 1.8295794397039753 >100 0.022378069016502418 2.455984737323426 >500 0.0022580068701843806 0.943578606397367 >1k 8.860949948642057E-4 0.9616169015392202 >5k 6.329249492012413E-5 0.2445901148967416 >10k+ 6.329249492012413E-5 0.865564781279017 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 30404 0.2965896964833089 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22816 0.22256908679657864 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15483 0.15103599100944193 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 9.754956468994505E-6 3 0.0 0.0 0.0 5.852973881396703E-5 9.754956468994505E-6 4 0.0 0.0 0.0 6.828469528296153E-5 9.754956468994505E-6 5 0.0 9.754956468994505E-6 0.0 7.803965175195604E-5 9.754956468994505E-6 6 0.0 9.754956468994505E-6 0.0 7.803965175195604E-5 9.754956468994505E-6 7 0.0 9.754956468994505E-6 0.0 7.803965175195604E-5 9.754956468994505E-6 8 0.0 9.754956468994505E-6 0.0 8.779460822095054E-5 9.754956468994505E-6 9 0.0 9.754956468994505E-6 0.0 9.754956468994504E-5 9.754956468994505E-6 10-11 0.0 9.754956468994505E-6 0.0 1.2681443409692856E-4 1.4632434703491758E-5 12-13 0.0 9.754956468994505E-6 0.0 1.9022165114539283E-4 1.950991293798901E-5 14-15 0.0 9.754956468994505E-6 0.0 2.1460904231787912E-4 2.4387391172486264E-5 16-17 0.0 9.754956468994505E-6 0.0 2.9752617230433237E-4 3.901982587597802E-5 18-19 0.0 9.754956468994505E-6 0.0 4.0483069346327195E-4 5.852973881396703E-5 20-21 0.0 9.754956468994505E-6 0.0 6.340721704846427E-4 7.803965175195604E-5 22-23 0.0 9.754956468994505E-6 0.0 0.0015412831221011318 1.0242704292444231E-4 24-25 0.0 1.4632434703491758E-5 0.0 0.0037410258058593925 1.0730452115893956E-4 26-27 0.0 1.950991293798901E-5 0.0 0.006589473094805788 1.0730452115893956E-4 28-29 0.0 1.950991293798901E-5 0.0 0.01622249260793786 1.1705947762793406E-4 30-31 0.0 1.950991293798901E-5 0.0 0.055803228480883066 1.1705947762793406E-4 32-33 0.0 1.950991293798901E-5 0.0 0.14261258609846517 1.1705947762793406E-4 34-35 0.0 3.901982587597802E-5 0.0 0.25866242573185827 1.1705947762793406E-4 36-37 0.0 4.389730411047527E-5 0.0 0.4334614906997708 1.1705947762793406E-4 38-39 0.0 6.828469528296153E-5 0.0 0.7460346833575273 1.1705947762793406E-4 40-41 0.0 8.779460822095054E-5 0.0 1.1477291583160174 1.3656939056592307E-4 42-43 9.754956468994505E-6 9.754956468994504E-5 0.0 1.5317427746744552 1.3656939056592307E-4 44-45 9.754956468994505E-6 9.754956468994504E-5 0.0 1.9286524434849035 1.5607930350391207E-4 46-47 9.754956468994505E-6 1.0242704292444231E-4 0.0 2.36787911345785 1.6095678173840933E-4 48-49 9.754956468994505E-6 1.1218199939343681E-4 0.0 2.8532174626597335 1.8534417291089558E-4 50-51 9.754956468994505E-6 1.1705947762793406E-4 0.0 3.39349084921676 1.8534417291089558E-4 52-53 9.754956468994505E-6 1.1705947762793406E-4 0.0 3.9626925591825892 2.3899643349036535E-4 54-55 9.754956468994505E-6 1.1705947762793406E-4 0.0 4.557593701965985 2.9264869406983514E-4 56-57 9.754956468994505E-6 1.1705947762793406E-4 0.0 5.141744882720548 2.9752617230433237E-4 58-59 9.754956468994505E-6 1.2193695586243131E-4 0.0 5.834307772193283 3.1215860700782415E-4 60-61 9.754956468994505E-6 1.3656939056592307E-4 0.0 6.510799370883348 3.3166851994581315E-4 62-63 9.754956468994505E-6 1.3656939056592307E-4 0.0 7.272968752240591 3.3166851994581315E-4 64-65 9.754956468994505E-6 1.4632434703491757E-4 0.0 7.961697943821011 3.365459981803104E-4 66-67 9.754956468994505E-6 1.5607930350391207E-4 0.0 8.707664342483255 3.4142347641480766E-4 68-69 9.754956468994505E-6 1.5607930350391207E-4 0.0 9.489860649471344 3.4142347641480766E-4 70-71 9.754956468994505E-6 1.5607930350391207E-4 0.0 10.287313708376942 3.6093338935279666E-4 72-73 9.754956468994505E-6 1.5607930350391207E-4 0.0 11.023744637090743 3.755658240562884E-4 74-75 9.754956468994505E-6 1.5607930350391207E-4 0.0 11.765604199079542 3.8044330229078567E-4 76-77 1.4632434703491758E-5 1.6583425997290658E-4 0.0 12.553936373686629 3.8044330229078567E-4 78-79 1.950991293798901E-5 1.6583425997290658E-4 0.0 13.361451670189993 3.8044330229078567E-4 80-81 1.950991293798901E-5 1.7071173820740383E-4 0.0 14.143843076307464 3.8044330229078567E-4 82-83 1.950991293798901E-5 1.9509912937989009E-4 0.0 14.943183719289813 3.9019825875978017E-4 84-85 2.4387391172486264E-5 1.9509912937989009E-4 0.0 15.71325461538694 3.9019825875978017E-4 86-87 2.9264869406983516E-5 1.9509912937989009E-4 0.0 16.48199883740429 3.9019825875978017E-4 88 2.9264869406983516E-5 1.9509912937989009E-4 0.0 17.06840341310319 3.9019825875978017E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 14795 0.0 51.742367 1 TACCTGG 10205 0.0 50.687366 2 GTATCAA 27315 0.0 41.633602 1 ACCTGGG 12265 0.0 41.3544 3 CAACGCA 33560 0.0 33.112606 5 ATCAACG 33630 0.0 33.00175 3 TATCAAC 34730 0.0 32.322033 2 GTACATG 37510 0.0 32.113792 1 TCAACGC 35110 0.0 31.730808 4 TACATGG 37720 0.0 31.531794 2 AACGCAG 36020 0.0 31.400051 6 GTACCTG 16780 0.0 31.331425 1 CCTGGGG 11940 0.0 30.786905 4 ACATGGG 38635 0.0 29.626879 3 CATGGGG 31095 0.0 28.148336 4 TGGGGGG 25970 0.0 27.875792 6 ACGCAGA 39725 0.0 27.856552 7 ATGGGGG 24970 0.0 27.259499 5 CGCAGAG 40495 0.0 27.196651 8 TAACGCA 1235 0.0 25.882462 4 >>END_MODULE