FastQCFastQC Report
Mon 6 Jun 2016
ERR1211429_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211429_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10251199
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT273210.26651516568939887No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT214650.20939014060696706No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153140.14938740336618184No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA145700.051.0506971
TACCTGG102550.050.4528852
ACCTGGG118400.042.508763
GTATCAA269600.042.0292741
CCTGGGG113250.033.0311784
ATCAACG343100.032.3382073
CAACGCA344300.032.1260385
TATCAAC353650.031.7318232
TCAACGC360600.030.8085254
GTACCTG169500.030.7898831
AACGCAG371350.030.2399466
TAACGCA13250.029.7928854
GTACATG378150.029.7407421
TGGGGGG232250.029.4558946
TACATGG384750.028.9592482
ACATGGG386100.027.8968723
CATGGGG303150.027.2217544
ATGGGGG235700.027.1522145
CTGGGGG84100.026.930155
ACGCAGA411000.026.8767877
Produced by FastQC (version 0.11.5)