##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211429_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10251199 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.06335395498614 33.0 31.0 34.0 30.0 34.0 2 32.20160139316386 33.0 31.0 34.0 30.0 34.0 3 32.277668690267355 34.0 31.0 34.0 30.0 34.0 4 35.92865390672837 37.0 35.0 37.0 35.0 37.0 5 35.78531077193995 37.0 35.0 37.0 35.0 37.0 6 35.819941842900526 37.0 35.0 37.0 35.0 37.0 7 35.75056332434869 37.0 35.0 37.0 33.0 37.0 8 35.73056244445162 37.0 35.0 37.0 33.0 37.0 9 37.31703111021452 39.0 37.0 39.0 34.0 39.0 10-11 37.28564278188337 39.0 37.0 39.0 34.0 39.0 12-13 37.19590201107207 39.0 37.0 39.0 33.0 39.0 14-15 38.39785985034531 40.0 38.0 41.0 33.5 41.0 16-17 38.38224772536364 40.0 38.0 41.0 33.0 41.0 18-19 38.385904565895174 40.0 38.0 41.0 33.5 41.0 20-21 38.34602474305689 40.0 38.0 41.0 34.0 41.0 22-23 38.247534556689416 40.0 38.0 41.0 34.0 41.0 24-25 38.13560818593025 40.0 37.0 41.0 33.0 41.0 26-27 38.01403435832238 40.0 37.0 41.0 33.0 41.0 28-29 37.93024572052498 40.0 37.0 41.0 33.0 41.0 30-31 37.98119307799995 40.0 37.0 41.0 33.0 41.0 32-33 37.8268963464664 40.0 37.0 41.0 33.0 41.0 34-35 37.72648194616064 40.0 37.0 41.0 33.0 41.0 36-37 37.59876883669901 40.0 37.0 41.0 32.5 41.0 38-39 37.48805695802022 40.0 36.0 41.0 32.0 41.0 40-41 37.42678675928542 40.0 36.0 41.0 32.0 41.0 42-43 37.30521946749839 40.0 36.0 41.0 31.5 41.0 44-45 37.16810828665018 39.5 36.0 41.0 31.0 41.0 46-47 36.96279781516289 39.0 36.0 41.0 31.0 41.0 48-49 36.75940453404524 39.0 35.0 41.0 30.0 41.0 50-51 36.589064215805394 39.0 35.0 41.0 30.0 41.0 52-53 36.391965710547616 39.0 35.0 40.0 30.0 41.0 54-55 36.19621075544431 38.5 35.0 40.0 29.0 41.0 56-57 35.9753640037619 38.0 35.0 40.0 29.0 41.0 58-59 35.716802102856455 38.0 34.5 40.0 28.0 41.0 60-61 35.45394338750033 38.0 34.0 40.0 28.0 41.0 62-63 35.142578590075175 37.0 34.0 40.0 27.5 41.0 64-65 34.79447501701996 37.0 34.0 39.5 26.5 41.0 66-67 34.448426276770164 36.0 33.0 39.0 26.0 41.0 68-69 34.086966948939335 36.0 33.0 39.0 26.0 40.0 70-71 33.717718337142806 35.0 33.0 38.5 26.0 40.0 72-73 33.3564255751937 35.0 32.0 37.5 25.5 40.0 74-75 32.98127658042732 35.0 32.0 37.0 25.0 39.0 76-77 31.970654213229107 34.0 30.5 36.0 24.5 38.5 78-79 32.46788839042145 35.0 32.0 36.0 25.0 38.5 80-81 32.370814818832415 35.0 32.0 36.0 25.0 37.5 82-83 32.09796146772685 34.5 31.5 35.0 25.0 37.0 84-85 31.8216644218886 34.0 31.0 35.0 24.0 36.5 86-87 31.574430269083646 34.0 31.0 35.0 24.0 36.0 88-89 31.361655548780195 34.0 31.0 35.0 23.0 36.0 90-91 31.153154084707552 34.0 31.0 35.0 23.0 35.0 92-93 30.972886635017034 34.0 30.5 35.0 22.0 35.0 94-95 30.811676614608693 34.0 30.0 35.0 20.0 35.0 96-97 30.646026625763483 34.0 30.0 35.0 20.0 35.0 98-99 30.468650789044286 34.0 30.0 35.0 20.0 35.0 100 28.61090785575424 32.0 25.0 34.0 17.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.27240757865891396 1101 2 -0.23409051709912632 1101 3 -0.30645912914010154 1101 4 -0.16702745956344245 1101 5 -0.19310194534693892 1101 6 -0.22737562351792207 1101 7 -0.25557558882465514 1101 8 -0.34027601043560907 1101 9 -0.4271532982172985 1101 10-11 -0.40968884995245247 1101 12-13 -0.5113060425881955 1101 14-15 -0.675421779877361 1101 16-17 -0.6402074217384808 1101 18-19 -0.6032878802063877 1101 20-21 -0.632417069155423 1101 22-23 -0.7204127297659397 1101 24-25 -0.7305527977930808 1101 26-27 -0.7161725623652515 1101 28-29 -0.7394166982927857 1101 30-31 -0.6578063633732469 1101 32-33 -0.5381303487524463 1101 34-35 -0.64714700455783 1101 36-37 -0.6836150461361896 1101 38-39 -0.7285255572655558 1101 40-41 -0.8262896948414635 1101 42-43 -0.7284985350038582 1101 44-45 -0.7574628664300107 1101 46-47 -0.8079421266728346 1101 48-49 -0.7707783334642215 1101 50-51 -0.8114932667774397 1101 52-53 -0.820107183512853 1101 54-55 -0.8203636116358126 1101 56-57 -0.9120787745356225 1101 58-59 -0.839658623470406 1101 60-61 -0.8795760273033153 1101 62-63 -0.9731102970785699 1101 64-65 -0.9718880328572723 1101 66-67 -0.9924635991818747 1101 68-69 -0.9729508058249152 1101 70-71 -1.062364844798239 1101 72-73 -1.0779547933812808 1101 74-75 -1.0571935217021853 1101 76-77 -0.8911889040261229 1101 78-79 -0.8270840235013743 1101 80-81 -0.7450801760564048 1101 82-83 -0.8706697356233697 1101 84-85 -0.8218827251359428 1101 86-87 -0.958861440821142 1101 88-89 -0.950467317777008 1101 90-91 -1.079729346258425 1101 92-93 -1.0386633718678624 1101 94-95 -1.0894442842941139 1101 96-97 -1.0564299652757576 1101 98-99 -1.144746792824062 1101 100 -1.1438857824639896 1102 1 -0.3033992733903901 1102 2 -0.25828965774944734 1102 3 -0.2870936584547046 1102 4 -0.12819847955076114 1102 5 -0.20811259528845483 1102 6 -0.2833374187370836 1102 7 -0.2683222561578944 1102 8 -0.3025297692306097 1102 9 -0.4530817733560468 1102 10-11 -0.4351950384187475 1102 12-13 -0.39098750432628293 1102 14-15 -0.537760440206192 1102 16-17 -0.6256073472295824 1102 18-19 -0.631092205663343 1102 20-21 -0.6702399842964937 1102 22-23 -0.6381755408888097 1102 24-25 -0.688954054180023 1102 26-27 -0.7275017091567193 1102 28-29 -0.7637338199202333 1102 30-31 -0.6497108012417812 1102 32-33 -0.7037427453976903 1102 34-35 -0.7000227336014575 1102 36-37 -0.6410923445768901 1102 38-39 -0.6767457531045338 1102 40-41 -0.8031178730102226 1102 42-43 -0.7879517661879802 1102 44-45 -0.7052958102164624 1102 46-47 -0.786285677484841 1102 48-49 -0.7899408948234807 1102 50-51 -0.922639584151554 1102 52-53 -0.8906243709843906 1102 54-55 -0.955462270874321 1102 56-57 -0.8423501399934352 1102 58-59 -0.9363445850993699 1102 60-61 -0.9863470861003591 1102 62-63 -1.010088149206176 1102 64-65 -1.0032065288911056 1102 66-67 -0.9877538153637886 1102 68-69 -1.0067721844326698 1102 70-71 -1.0472703274562605 1102 72-73 -1.0717542952297876 1102 74-75 -1.0893728142668166 1102 76-77 -0.8842411599863276 1102 78-79 -0.8584669284396114 1102 80-81 -0.7571997295225117 1102 82-83 -0.8114399098924494 1102 84-85 -0.9202529484278408 1102 86-87 -0.9363116846073005 1102 88-89 -0.775625735467603 1102 90-91 -0.7647366694849822 1102 92-93 -0.8945605471587861 1102 94-95 -0.9484107196359126 1102 96-97 -1.0903332351215376 1102 98-99 -1.0629425467400502 1102 100 -1.1461789819632848 1103 1 -0.33224449165753 1103 2 -0.2556483712691886 1103 3 -0.30535883707530687 1103 4 -0.13508157854737846 1103 5 -0.11686502868530368 1103 6 -0.28283494839526213 1103 7 -0.29726959113789775 1103 8 -0.3245227982422634 1103 9 -0.48056522112537436 1103 10-11 -0.3696698242241254 1103 12-13 -0.48140311490525534 1103 14-15 -0.6486305193745281 1103 16-17 -0.6659742299577545 1103 18-19 -0.6595489770985594 1103 20-21 -0.6296799215314834 1103 22-23 -0.7585360153402476 1103 24-25 -0.7111128138819964 1103 26-27 -0.8278496927526291 1103 28-29 -0.8507889255471994 1103 30-31 -0.6754776594689531 1103 32-33 -0.6838162909415217 1103 34-35 -0.6877654780921034 1103 36-37 -0.6094995846717097 1103 38-39 -0.6678615055608574 1103 40-41 -0.7677544870926454 1103 42-43 -0.8107967361718025 1103 44-45 -0.7539067674835422 1103 46-47 -0.8159284874856709 1103 48-49 -0.8091341430979 1103 50-51 -0.7481910141087766 1103 52-53 -0.8502249886040474 1103 54-55 -0.855451886183296 1103 56-57 -0.9437747096392997 1103 58-59 -0.9328410992135687 1103 60-61 -0.9538543313308239 1103 62-63 -1.0303092457427994 1103 64-65 -0.9997026630633243 1103 66-67 -0.9878374435155308 1103 68-69 -0.9633875336419564 1103 70-71 -0.9686527920491486 1103 72-73 -0.9407823044003507 1103 74-75 -1.0543067709403608 1103 76-77 -0.9229886189489989 1103 78-79 -0.7650126646798938 1103 80-81 -0.736785090542849 1103 82-83 -0.7177124503155561 1103 84-85 -0.7260964957803075 1103 86-87 -0.8295083231565847 1103 88-89 -0.8629569536970756 1103 90-91 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-0.8654320111475613 1104 48-49 -0.9143989099616903 1104 50-51 -0.9286983185043383 1104 52-53 -0.8182998139007083 1104 54-55 -0.7658388257277906 1104 56-57 -0.7861725098029595 1104 58-59 -0.9074969371064512 1104 60-61 -1.0432522309782613 1104 62-63 -1.061252155442908 1104 64-65 -0.9722250073774745 1104 66-67 -1.0665220517691694 1104 68-69 -0.9117822372185103 1104 70-71 -0.9622873957513747 1104 72-73 -0.928815503482376 1104 74-75 -0.9278256943348921 1104 76-77 -0.8723624750111902 1104 78-79 -0.8777177676180337 1104 80-81 -0.8632073972101324 1104 82-83 -0.8620748412177264 1104 84-85 -0.7764274585937656 1104 86-87 -0.786701530005768 1104 88-89 -0.8688864398621163 1104 90-91 -0.8365195150399032 1104 92-93 -0.8983270244503565 1104 94-95 -1.0050438319788206 1104 96-97 -1.0642664427961073 1104 98-99 -1.091090329838888 1104 100 -1.1230188935843053 1105 1 -0.3601646689347504 1105 2 -0.35992645857243133 1105 3 -0.3892073809446437 1105 4 -0.27417297555994224 1105 5 -0.26039536754053927 1105 6 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1105 68-69 -1.0332628839714815 1105 70-71 -1.0341688388895207 1105 72-73 -1.0632508211150125 1105 74-75 -0.996323243464861 1105 76-77 -1.0054584975701388 1105 78-79 -0.8917909382106828 1105 80-81 -0.8081059188856976 1105 82-83 -0.7815916157617053 1105 84-85 -0.7239650083953357 1105 86-87 -0.8603965410968257 1105 88-89 -0.8468942095962007 1105 90-91 -0.8661007959928355 1105 92-93 -0.9144114720933842 1105 94-95 -0.8714158152708755 1105 96-97 -0.8860448563296437 1105 98-99 -0.9147844206219311 1105 100 -0.9180271518897136 1106 1 -0.3527954823811257 1106 2 -0.31978831835193233 1106 3 -0.3940720536820663 1106 4 -0.24956197057075968 1106 5 -0.25295138683437557 1106 6 -0.32538898889557544 1106 7 -0.38918267930912265 1106 8 -0.35350648965206233 1106 9 -0.5312212294577989 1106 10-11 -0.5038800250918527 1106 12-13 -0.5812426215403335 1106 14-15 -0.6698692869379741 1106 16-17 -0.821146437959797 1106 18-19 -0.7566501575255273 1106 20-21 -0.705784245906095 1106 22-23 -0.6975694187912183 1106 24-25 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0.9561196777062406 2311 36-37 1.020560809732082 2311 38-39 1.0854116553320665 2311 40-41 1.0019382937109356 2311 42-43 1.1256468424284947 2311 44-45 1.0688173920744148 2311 46-47 1.0987815812155333 2311 48-49 1.0641123203578857 2311 50-51 1.023990755085883 2311 52-53 1.0467747721511529 2311 54-55 1.0588100604249746 2311 56-57 1.0842397584332275 2311 58-59 1.1070896233353764 2311 60-61 1.1388216658954136 2311 62-63 1.1863534780250902 2311 64-65 1.1837325785927746 2311 66-67 1.20053831403051 2311 68-69 1.2244016094734533 2311 70-71 1.2186170070865643 2311 72-73 1.2102197486266704 2311 74-75 1.1573721312132008 2311 76-77 1.0240378332064068 2311 78-79 0.9921297933993074 2311 80-81 1.0335536814174802 2311 82-83 0.9864637545257082 2311 84-85 0.9918192218862352 2311 86-87 1.0554287513254508 2311 88-89 1.1170233527858038 2311 90-91 1.1554924526866515 2311 92-93 1.1861097839897319 2311 94-95 1.177516656968141 2311 96-97 1.2548899896309003 2311 98-99 1.2707814722125939 2311 100 1.3992306410745456 2312 1 0.2088012282903975 2312 2 0.20153129277757387 2312 3 0.36663895580863937 2312 4 0.2716687686832273 2312 5 0.3734733393423042 2312 6 0.44033121126444286 2312 7 0.49435859076206157 2312 8 0.31683184078440263 2312 9 0.48447427832237366 2312 10-11 0.38252235379133026 2312 12-13 0.5164144388939249 2312 14-15 0.8437179709663454 2312 16-17 0.9047210025853758 2312 18-19 0.9165855960748743 2312 20-21 0.9138048553050737 2312 22-23 0.5186368608672325 2312 24-25 0.4930984393555775 2312 26-27 0.8683483554717739 2312 28-29 0.9972881895863068 2312 30-31 0.9098057119511296 2312 32-33 0.7714379973629875 2312 34-35 0.927477259869157 2312 36-37 1.047676618473119 2312 38-39 0.9759377752170195 2312 40-41 0.998462263524857 2312 42-43 0.8725773181270498 2312 44-45 0.8338805741472868 2312 46-47 1.057028279944305 2312 48-49 1.1005397377087078 2312 50-51 1.1709074102099137 2312 52-53 1.1731779385779504 2312 54-55 1.1458648612410514 2312 56-57 1.1152431702329082 2312 58-59 1.1651581253424652 2312 60-61 1.1702241325357292 2312 62-63 1.1774404168620904 2312 64-65 1.2521144862457518 2312 66-67 1.228012857177255 2312 68-69 1.2424491084332274 2312 70-71 1.0400912231102382 2312 72-73 1.1509454981057772 2312 74-75 1.1678789095224502 2312 76-77 1.1202458036882632 2312 78-79 1.0579592805945524 2312 80-81 0.8992997280408943 2312 82-83 0.8930922060480739 2312 84-85 0.7700776885381124 2312 86-87 0.973975377095158 2312 88-89 0.5935607936980496 2312 90-91 0.9673261715744417 2312 92-93 1.1682477409590568 2312 94-95 1.2366393843927703 2312 96-97 1.2830732368815063 2312 98-99 1.297679198904632 2312 100 1.3832502090730223 2313 1 -0.36815761317849294 2313 2 -0.016573227109063282 2313 3 0.25190348408442986 2313 4 0.09437004598535026 2313 5 0.21624028290329278 2313 6 0.3483251230390181 2313 7 0.11571871594924943 2313 8 0.11739699047515018 2313 9 0.2845704491516514 2313 10-11 0.40471259460895936 2313 12-13 0.5736369397500383 2313 14-15 0.6093491943493916 2313 16-17 0.7548479352829176 2313 18-19 0.6353070173577748 2313 20-21 0.8484038111394696 2313 22-23 0.8960796262979187 2313 24-25 0.7284993049852204 2313 26-27 0.9305713273401821 2313 28-29 0.8518134953372751 2313 30-31 0.8255421231106936 2313 32-33 0.9633130019636411 2313 34-35 0.9760426034415488 2313 36-37 0.8417246463549901 2313 38-39 0.6348279456546067 2313 40-41 0.7834563186297885 2313 42-43 0.8335103215787072 2313 44-45 1.0206998524143742 2313 46-47 1.0299497732895802 2313 48-49 1.0694879450768369 2313 50-51 0.991619221432039 2313 52-53 0.895402646867872 2313 54-55 0.9878570848001829 2313 56-57 1.1076039458359688 2313 58-59 0.9565250500792288 2313 60-61 1.0166309119352732 2313 62-63 1.100999475412813 2313 64-65 1.1537697850609376 2313 66-67 1.1312426683015246 2313 68-69 1.1471860945273917 2313 70-71 1.0909709322893804 2313 72-73 1.0941072257542288 2313 74-75 0.7852934407853667 2313 76-77 0.9657902591360283 2313 78-79 0.8070628219766789 2313 80-81 0.9134659669284346 2313 82-83 0.9655221785027024 2313 84-85 0.9697324349627614 2313 86-87 1.0702160312899593 2313 88-89 1.0183800456931031 2313 90-91 1.115962060575285 2313 92-93 0.8225148772019679 2313 94-95 0.892372962429274 2313 96-97 1.187251017027716 2313 98-99 1.2723788331607686 2313 100 1.385590263795038 2314 1 -0.3041395313141635 2314 2 -0.12701490959549488 2314 3 0.10574628700961597 2314 4 -0.15801030369969027 2314 5 0.18816966316153128 2314 6 0.1823880479370672 2314 7 0.07734149594229223 2314 8 0.14494521605475086 2314 9 0.2769067598725954 2314 10-11 0.45334968323125935 2314 12-13 0.5464821786599785 2314 14-15 0.5467237451117413 2314 16-17 0.7912677657030329 2314 18-19 0.8418522390487553 2314 20-21 0.515387686581029 2314 22-23 0.7145636472908734 2314 24-25 0.8661770225973839 2314 26-27 0.8556724073397888 2314 28-29 0.8073512380292556 2314 30-31 0.7815739411005609 2314 32-33 0.8984372960668239 2314 34-35 0.8935367390195452 2314 36-37 0.7978304402218725 2314 38-39 0.855584024714453 2314 40-41 0.8830291815074816 2314 42-43 0.7315336762354505 2314 44-45 0.7870981771695895 2314 46-47 0.8811721087403939 2314 48-49 0.8302360789192491 2314 50-51 0.9415084671874894 2314 52-53 1.077971452217085 2314 54-55 1.0637095862283275 2314 56-57 0.9675750561083944 2314 58-59 0.952631907602516 2314 60-61 1.0384880903046962 2314 62-63 0.7531483301834143 2314 64-65 0.8865700253993438 2314 66-67 0.9756746596768977 2314 68-69 1.0142662139340999 2314 70-71 1.0952826810774425 2314 72-73 1.0819213261998257 2314 74-75 1.0845042701333156 2314 76-77 0.8705096576236642 2314 78-79 0.8627095676899046 2314 80-81 0.9065412128658323 2314 82-83 0.8549535817067451 2314 84-85 0.7387404473433286 2314 86-87 0.9415957771762464 2314 88-89 0.9906371735351662 2314 90-91 1.0661254693606637 2314 92-93 1.1061375761653558 2314 94-95 1.1443052185637619 2314 96-97 1.2485116838138701 2314 98-99 1.2586206926255379 2314 100 1.2535223963563027 2315 1 -0.11606560202508831 2315 2 0.10774272881889857 2315 3 0.10678883412057871 2315 4 -0.046364515950628515 2315 5 0.25926738757245005 2315 6 0.35782792385926854 2315 7 0.4113435235267815 2315 8 0.3801199023788584 2315 9 0.5735664058738692 2315 10-11 0.5375198917565953 2315 12-13 0.6027006847603289 2315 14-15 0.6885431413564476 2315 16-17 0.7088992280605169 2315 18-19 0.7626329633842275 2315 20-21 0.772690971062211 2315 22-23 0.8371952362477018 2315 24-25 0.782051875248527 2315 26-27 0.8819459632489739 2315 28-29 0.8981746237354784 2315 30-31 0.9060154911661868 2315 32-33 0.9415260989791392 2315 34-35 0.8888912266711344 2315 36-37 0.9308802339547739 2315 38-39 0.9541630918727293 2315 40-41 0.9698397873712636 2315 42-43 1.024029534772147 2315 44-45 0.8917945161998588 2315 46-47 0.901654806388116 2315 48-49 0.9737778802566694 2315 50-51 0.9127989522335511 2315 52-53 0.9244437863425006 2315 54-55 0.888409815125101 2315 56-57 1.012847154437317 2315 58-59 1.020937160222978 2315 60-61 1.0617574962192435 2315 62-63 1.0797254133948329 2315 64-65 1.0600921441549218 2315 66-67 1.1139287633205868 2315 68-69 1.0127336913478828 2315 70-71 0.6483165810929705 2315 72-73 0.8977486880767884 2315 74-75 1.0622042855914557 2315 76-77 0.991771562528541 2315 78-79 0.8361011494060264 2315 80-81 0.8633179233315147 2315 82-83 0.8843049682325059 2315 84-85 0.8075580062031271 2315 86-87 0.777380051412905 2315 88-89 0.907595406788797 2315 90-91 0.9989445134061867 2315 92-93 0.9821949787997752 2315 94-95 1.0300543412514251 2315 96-97 1.1362667307168302 2315 98-99 1.0049966500665413 2315 100 0.8837098954393419 2316 1 0.44640565617907413 2316 2 0.46878559990160085 2316 3 0.4602461978917418 2316 4 0.2959011511151104 2316 5 0.3880431881075026 2316 6 0.41212497781288704 2316 7 0.4559135367005993 2316 8 0.4471841971410342 2316 9 0.5012305033609508 2316 10-11 0.47335583188121433 2316 12-13 0.6200226634752113 2316 14-15 0.7719950359991046 2316 16-17 0.7806022657729415 2316 18-19 0.8235369114737665 2316 20-21 0.8395247236430521 2316 22-23 0.8681152560318139 2316 24-25 0.9105379001692882 2316 26-27 0.9476985909217461 2316 28-29 0.9113706302643507 2316 30-31 0.8066127335986906 2316 32-33 0.7346980135097354 2316 34-35 0.6654827912794516 2316 36-37 0.6675277990554704 2316 38-39 0.5929902434349756 2316 40-41 0.7007497408590737 2316 42-43 0.8614587893463508 2316 44-45 0.8269644497993411 2316 46-47 0.8669343814487149 2316 48-49 0.6360216562461716 2316 50-51 0.7348503579387398 2316 52-53 0.876456177251093 2316 54-55 0.9979408154333953 2316 56-57 1.0169122185345003 2316 58-59 0.9543529471956518 2316 60-61 1.0296366785290374 2316 62-63 1.0404554486302828 2316 64-65 1.047347313344325 2316 66-67 1.0582124495948761 2316 68-69 1.049334504741843 2316 70-71 0.8605044707452691 2316 72-73 0.9323246240873786 2316 74-75 1.0403722338468242 2316 76-77 0.8730178651083875 2316 78-79 0.8311927520375875 2316 80-81 0.8484899978946387 2316 82-83 0.757111869450732 2316 84-85 0.7746080312511019 2316 86-87 0.7640100896095561 2316 88-89 0.7265624446037329 2316 90-91 0.7644291049671459 2316 92-93 0.845432662099963 2316 94-95 0.7130409493798062 2316 96-97 0.8958497495087414 2316 98-99 0.9236142407012125 2316 100 0.5887594995377121 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 11.0 12 57.0 13 268.0 14 1085.0 15 2957.0 16 7107.0 17 14282.0 18 23712.0 19 33570.0 20 45304.0 21 60785.0 22 73815.0 23 80766.0 24 84525.0 25 94332.0 26 111636.0 27 135793.0 28 167105.0 29 202945.0 30 248291.0 31 308329.0 32 388762.0 33 506618.0 34 712514.0 35 959975.0 36 1448757.0 37 2231270.0 38 2005124.0 39 301454.0 40 49.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.7320709666696 19.698559231887042 13.251746316828442 24.317623484614913 2 15.67004168683504 20.667291764338177 40.5201421336316 23.142524415195183 3 17.483332192003314 27.49301106075602 31.053316890377104 23.97033985686356 4 12.169352124218875 17.32548916304459 38.45841565321743 32.04674305951911 5 12.44857703372527 38.0264799619931 34.34755632991473 15.177386674366902 6 29.285308897555197 37.44603313680996 19.55750927842775 13.711148687207094 7 26.711858858018406 33.301958086585344 23.08286235507889 16.90332070031736 8 26.399486340757345 35.531999616823626 20.489810220390797 17.57870382202823 9 28.029952141347884 15.349057947404257 20.2005263866425 36.42046352460536 10-11 24.97291880881096 25.875605671712798 29.144463601703563 20.00701191777268 12-13 25.169845255216977 24.044958967852402 28.517760827191474 22.267434949739147 14-15 23.007291051697774 25.35104684370487 26.42075129575125 25.22091080884611 16-17 22.423978212479632 27.532253696022302 27.209325317808243 22.834442773689826 18-19 22.024887040042827 27.21986472021468 28.823057673546288 21.93219056619621 20-21 23.019020654024015 26.70454093733528 28.189357566337243 22.087080842303465 22-23 22.7164533704477 26.529427415675094 28.194735297998115 22.559383915879096 24-25 22.08263467481297 26.250266676278557 28.704776117277277 22.962322531631195 26-27 22.233584578740498 26.47562007136921 28.309849413712485 22.980945936177807 28-29 22.16524185952818 27.464290400196273 28.189403180830926 22.181064559444625 30-31 23.291356765448757 26.511259847220536 28.41929112888782 21.778092258442886 32-33 23.147689784271375 26.56557352898421 28.01487186801878 22.271864818725632 34-35 22.88462365940766 26.843298918978885 28.436803249825033 21.835274171788424 36-37 22.644051909486848 26.820861061112904 28.207131319167388 22.327955710232857 38-39 22.73602131603531 26.954717523651027 28.217032465653002 22.092228694660662 40-41 22.764440268884023 26.232590367231186 28.704304492392613 22.29866487149218 42-43 22.49813262721913 26.585442657339996 27.99994400564314 22.916480709797735 44-45 21.788190777525955 27.010608431429382 28.5587180713667 22.64248271967796 46-47 21.79228543100389 26.337244399565357 28.19995585263561 23.670514316795146 48-49 22.133316775038626 26.413117291161843 28.324278463040354 23.12928747075918 50-51 22.083867490541508 26.8191933967218 28.05640563535387 23.040533477382816 52-53 21.754992097515377 27.06064500216569 27.808545571596305 23.37581732872263 54-55 21.591243951517214 26.621650935501762 28.333272114982794 23.453832997998234 56-57 22.15367156495465 26.476982081905142 27.807280623169035 23.562065729971174 58-59 21.477573668789315 26.698771736456095 28.44367335303597 23.37998124171862 60-61 22.225351983524025 27.025334385323337 27.69876796987001 23.05054566128263 62-63 22.2712679168612 26.855435388482285 27.516793810770316 23.356502883886193 64-65 21.688524367037648 26.42694201201225 27.660949862217237 24.223583758732865 66-67 21.647191020666202 26.282655665041986 27.59424495368229 24.475908360609516 68-69 21.14353338473701 26.50615380110681 27.90482836702226 24.445484447133918 70-71 21.477594720510005 26.20977861571151 27.982928781060334 24.329697882718154 72-73 21.492973081586108 26.72832165063185 27.309557539059348 24.46914772872269 74-75 21.695071393422925 26.629181293813748 27.07360880328771 24.602138509475623 76-77 21.271564831810338 26.439259778729063 27.374459924781974 24.914715464678626 78-79 21.045387398919832 26.40966137414737 27.679299174568357 24.865652052364442 80-81 21.126367471780892 26.654175838749598 27.360439434694516 24.85901725477499 82-83 21.616025522683817 26.388765835074405 27.44532052255306 24.54988811968871 84-85 21.66284817609596 26.663300285509656 26.870646007015043 24.803205531379348 86-87 21.07202183259656 26.799804085960332 27.148782678833168 24.97939140260994 88-89 21.15247018377693 26.31340402223691 27.219953657410656 25.314172136575504 90-91 21.651068029548203 25.92897771101438 27.357382274453716 25.062571984983702 92-93 21.568206253965798 26.236014023030922 27.064724841097327 25.13105488190595 94-95 20.921979726966505 26.02232445282477 27.34878635737855 25.70690946283017 96-97 20.865145137038194 25.8509285062458 27.526775471664706 25.7571508850513 98-99 20.754418746106012 26.15832419949446 27.30855124899545 25.77870580540408 100 20.635553428588295 26.126456009097176 27.39523100767025 25.842759554644278 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1158.0 1 834.5 2 1420.0 3 2273.0 4 1701.5 5 1283.0 6 1777.0 7 3174.0 8 4876.5 9 5784.5 10 5560.5 11 5252.5 12 5793.0 13 6650.5 14 7565.5 15 8645.5 16 9800.0 17 10885.0 18 11852.5 19 12891.0 20 14114.5 21 15654.0 22 18008.0 23 21486.5 24 26026.0 25 31800.0 26 40257.0 27 50269.5 28 62458.5 29 79707.5 30 100297.5 31 120037.0 32 144964.5 33 169773.0 34 190532.5 35 213033.5 36 237641.5 37 269130.5 38 287119.5 39 287360.0 40 287722.0 41 291930.0 42 301847.5 43 316089.0 44 334861.0 45 362773.5 46 387915.5 47 411004.5 48 438151.5 49 458201.5 50 469992.0 51 471106.5 52 454414.5 53 424029.0 54 391710.5 55 353428.5 56 313874.0 57 271999.5 58 228626.5 59 191744.0 60 151444.5 61 114480.5 62 88481.5 63 68017.5 64 49569.0 65 34383.0 66 24452.0 67 18458.0 68 14111.5 69 10515.0 70 7774.5 71 5694.5 72 4293.0 73 3178.0 74 2275.5 75 1555.5 76 1043.0 77 624.5 78 401.0 79 270.0 80 161.5 81 100.0 82 71.5 83 53.5 84 32.5 85 19.0 86 16.0 87 10.0 88 5.0 89 5.0 90 5.0 91 3.5 92 1.5 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.050784303377585396 2 9.754956468994505E-6 3 9.754956468994505E-6 4 0.015110427570472488 5 0.004184876325198642 6 9.754956468994505E-6 7 1.7558921644190108E-4 8 4.389730411047527E-4 9 0.0017461372079500164 10-11 7.852739957540576E-4 12-13 2.877712158353379E-4 14-15 2.9264869406983516E-5 16-17 9.267208645544779E-5 18-19 0.0 20-21 4.877478234497252E-6 22-23 1.4632434703491758E-5 24-25 5.852973881396703E-5 26-27 0.0 28-29 0.0010145154727754285 30-31 8.09661386926544E-4 32-33 0.013432575057805433 34-35 0.022221790836369482 36-37 0.029781882099840222 38-39 0.019641604850320436 40-41 1.3169191233142582E-4 42-43 0.001619322773853088 44-45 0.009325738384358746 46-47 0.014012994967710607 48-49 0.0053262062320709996 50-51 0.009925668207201909 52-53 0.015359178960431846 54-55 0.00979885377310498 56-57 0.0029703842448088264 58-59 0.007735680479912643 60-61 0.0014729984268181703 62-63 9.852506033684448E-4 64-65 0.01865635424695199 66-67 0.04360465541640544 68-69 0.05364250562300078 70-71 0.047121317223477954 72-73 0.039688040394104146 74-75 0.027762606110758363 76-77 0.027879665588386296 78-79 0.01198396402215975 80-81 0.002053418336723343 82-83 0.026796865420327904 84-85 0.04668234418237321 86-87 0.04749200556929974 88-89 0.058329762206352644 90-91 0.03318636190751931 92-93 0.02565553551345555 94-95 0.007169893004710962 96-97 0.003438622155320563 98-99 0.021680390752340288 100 0.018183238858205757 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.0251199E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.42198401012831 #Duplication Level Percentage of deduplicated Percentage of total 1 75.16719429089694 43.9141662294899 2 14.479615666390098 16.918557498692916 3 4.769508369165268 8.359324250386395 4 2.0919434515867046 4.888619475147644 5 1.041071536133709 3.041073231870164 6 0.5966351574322124 2.0913965776431063 7 0.3956634584000622 1.6180810968028352 8 0.2666659929316397 1.2463325100077796 9 0.20600618696617162 1.0831761144832661 >10 0.9114806283843366 9.25048637355715 >50 0.046560689200036136 1.8699763279651376 >100 0.024177191268986882 2.619707517088081 >500 0.002397605383954457 0.9933788226989333 >1k 9.502027611194735E-4 1.0668158804755976 >5k 6.478654931307491E-5 0.23018857452662367 >10k+ 6.478654931307491E-5 0.8087195191645151 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 27321 0.26651516568939887 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21465 0.20939014060696706 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15314 0.14938740336618184 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.950991293798901E-5 2 0.0 0.0 0.0 0.0 1.950991293798901E-5 3 0.0 0.0 0.0 1.950991293798901E-5 1.950991293798901E-5 4 0.0 0.0 0.0 1.950991293798901E-5 2.9264869406983516E-5 5 0.0 0.0 0.0 3.901982587597802E-5 2.9264869406983516E-5 6 0.0 0.0 0.0 3.901982587597802E-5 3.901982587597802E-5 7 0.0 0.0 0.0 3.901982587597802E-5 3.901982587597802E-5 8 0.0 0.0 0.0 4.877478234497252E-5 3.901982587597802E-5 9 0.0 0.0 0.0 5.852973881396703E-5 3.901982587597802E-5 10-11 0.0 0.0 0.0 8.291712998645329E-5 3.901982587597802E-5 12-13 0.0 0.0 0.0 1.3656939056592307E-4 4.877478234497253E-5 14-15 0.0 0.0 0.0 1.6583425997290658E-4 8.779460822095054E-5 16-17 0.0 0.0 0.0 2.1948652055237634E-4 1.0730452115893956E-4 18-19 0.0 0.0 0.0 3.2191356347681865E-4 1.0730452115893956E-4 20-21 0.0 0.0 0.0 5.41400084029195E-4 1.1218199939343681E-4 22-23 0.0 0.0 0.0 0.0014486110356456841 1.2681443409692856E-4 24-25 0.0 0.0 0.0 0.003667863632341934 1.3656939056592307E-4 26-27 0.0 0.0 0.0 0.0065163109212883295 1.3656939056592307E-4 28-29 0.0 0.0 0.0 0.016183472782061885 1.4632434703491757E-4 30-31 0.0 0.0 0.0 0.05590077804557301 1.4632434703491757E-4 32-33 0.0 0.0 0.0 0.14287109244489352 1.4632434703491757E-4 34-35 0.0 0.0 0.0 0.25944282224937787 1.5607930350391207E-4 36-37 0.0 0.0 0.0 0.4345735557372362 1.5607930350391207E-4 38-39 0.0 9.754956468994505E-6 0.0 0.7482539359542235 1.5607930350391207E-4 40-41 0.0 9.754956468994505E-6 0.0 1.1515335913389253 1.7071173820740383E-4 42-43 0.0 9.754956468994505E-6 0.0 1.5370006962112432 1.7558921644190108E-4 44-45 0.0 9.754956468994505E-6 0.0 1.9351151021456126 1.8046669467639833E-4 46-47 0.0 1.4632434703491758E-5 0.0 2.375561141677183 2.2436399878687362E-4 48-49 0.0 1.950991293798901E-5 0.0 2.861050692704336 2.2924147702137087E-4 50-51 0.0 1.950991293798901E-5 0.0 3.4008899836984923 2.3411895525586812E-4 52-53 0.0 1.950991293798901E-5 0.0 3.9703111801848743 2.7801625936634336E-4 54-55 0.0 1.950991293798901E-5 0.0 4.565109895925344 2.9264869406983514E-4 56-57 0.0 1.950991293798901E-5 0.0 5.14841727294534 2.9264869406983514E-4 58-59 0.0 1.950991293798901E-5 0.0 5.83995589198883 2.9264869406983514E-4 60-61 0.0 1.950991293798901E-5 0.0 6.516169474419529 2.9752617230433237E-4 62-63 0.0 1.950991293798901E-5 0.0 7.277460909694563 3.0240365053882964E-4 64-65 0.0 2.4387391172486264E-5 0.0 7.964678083022289 3.0240365053882964E-4 66-67 0.0 2.9264869406983516E-5 0.0 8.708912976911286 3.072811287733269E-4 68-69 0.0 2.9264869406983516E-5 0.0 9.489650917907262 3.1215860700782415E-4 70-71 0.0 3.414234764148077E-5 0.0 10.285465144126068 3.1215860700782415E-4 72-73 0.0 3.901982587597802E-5 0.0 11.021657076406381 3.6581086758729394E-4 74-75 0.0 3.901982587597802E-5 0.0 11.764409216912089 3.7068834582179116E-4 76-77 0.0 3.901982587597802E-5 0.0 12.553570562819043 3.7068834582179116E-4 78-79 0.0 3.901982587597802E-5 0.0 13.361339488190602 3.8044330229078567E-4 80-81 0.0 5.365226057946978E-5 0.0 14.143365083440482 3.9507573699427745E-4 82-83 0.0 5.852973881396703E-5 0.0 14.94280327598752 4.2434060640126096E-4 84-85 0.0 5.852973881396703E-5 0.0 15.713215595561065 4.2921808463575824E-4 86-87 0.0 5.852973881396703E-5 0.0 16.482901170877668 4.2921808463575824E-4 88 0.0 5.852973881396703E-5 0.0 17.069222829446584 4.2921808463575824E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 14570 0.0 51.050697 1 TACCTGG 10255 0.0 50.452885 2 ACCTGGG 11840 0.0 42.50876 3 GTATCAA 26960 0.0 42.029274 1 CCTGGGG 11325 0.0 33.031178 4 ATCAACG 34310 0.0 32.338207 3 CAACGCA 34430 0.0 32.126038 5 TATCAAC 35365 0.0 31.731823 2 TCAACGC 36060 0.0 30.808525 4 GTACCTG 16950 0.0 30.789883 1 AACGCAG 37135 0.0 30.239946 6 TAACGCA 1325 0.0 29.792885 4 GTACATG 37815 0.0 29.740742 1 TGGGGGG 23225 0.0 29.455894 6 TACATGG 38475 0.0 28.959248 2 ACATGGG 38610 0.0 27.896872 3 CATGGGG 30315 0.0 27.221754 4 ATGGGGG 23570 0.0 27.152214 5 CTGGGGG 8410 0.0 26.93015 5 ACGCAGA 41100 0.0 26.876787 7 >>END_MODULE