FastQCFastQC Report
Mon 6 Jun 2016
ERR1211428_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211428_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12418729
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT314620.2533431561313561No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT229620.18489814859475556No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172200.1386615329153249No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG150850.056.2813452
ACCTGGG177100.047.0327343
GGTATCA167550.045.8632351
GTATCAA302500.042.457621
GTACCTG234800.036.8207971
CCTGGGG171300.036.2762344
CAACGCA380600.032.9758385
ATCAACG380800.032.909273
GTACATG446750.032.629541
TATCAAC393950.032.374382
TACATGG454050.031.7480132
TCAACGC398100.031.6321414
AACGCAG410000.031.1736346
TGGGGGG317000.031.1550796
CTGGGGG125200.031.0494945
ACATGGG462000.030.1266963
CATGGGG367600.029.6661054
ATGGGGG290200.029.1236345
TAACGCA14350.028.4980684
ATAACGC14350.027.843393
Produced by FastQC (version 0.11.5)