FastQCFastQC Report
Mon 6 Jun 2016
ERR1211428_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211428_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12418729
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290440.2338725645756502No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225820.18183825414017812No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172620.138999731776094No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG146800.055.3513262
GGTATCA175250.048.079451
ACCTGGG166800.048.0106773
GTATCAA302500.039.947281
CCTGGGG157950.037.371574
GTACCTG239200.034.458811
CTGGGGG112400.033.193165
TGGGGGG272050.032.6954656
GTACATG447250.030.9714761
CAACGCA386450.030.3490875
ATCAACG387900.030.2283973
TACATGG451700.030.140992
TATCAAC402000.029.6354142
TAACGCA15400.029.296914
TCAACGC402550.029.2338374
ATAACGC15100.028.9447863
ACATGGG448700.028.9289023
AACGCAG416350.028.7897246
ATGGGGG267650.028.7041655
CATGGGG355900.028.2326094
Produced by FastQC (version 0.11.5)