##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211428_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 12418729 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.064436948418795 33.0 31.0 34.0 30.0 34.0 2 32.19571841852737 33.0 31.0 34.0 30.0 34.0 3 32.27576638478865 34.0 31.0 34.0 30.0 34.0 4 35.926573484291346 37.0 35.0 37.0 35.0 37.0 5 35.785725817835306 37.0 35.0 37.0 35.0 37.0 6 35.82018465818845 37.0 35.0 37.0 35.0 37.0 7 35.747025883244575 37.0 35.0 37.0 33.0 37.0 8 35.72698703707924 37.0 35.0 37.0 33.0 37.0 9 37.31404228242681 39.0 37.0 39.0 34.0 39.0 10-11 37.283649598924335 39.0 37.0 39.0 34.0 39.0 12-13 37.18786515914793 39.0 37.0 39.0 33.0 39.0 14-15 38.393923927319776 40.0 38.0 41.0 33.5 41.0 16-17 38.38156187319974 40.0 38.0 41.0 33.0 41.0 18-19 38.377750130468264 40.0 38.0 41.0 33.5 41.0 20-21 38.33515821144016 40.0 38.0 41.0 34.0 41.0 22-23 38.23549426837481 40.0 38.0 41.0 34.0 41.0 24-25 38.12246985983831 40.0 37.0 41.0 33.0 41.0 26-27 38.00618271805432 40.0 37.0 41.0 33.0 41.0 28-29 37.926316533680705 40.0 37.0 41.0 33.0 41.0 30-31 37.97733093298034 40.0 37.0 41.0 33.0 41.0 32-33 37.82354414046719 40.0 37.0 41.0 33.0 41.0 34-35 37.72140071661117 40.0 37.0 41.0 33.0 41.0 36-37 37.59075944889368 40.0 37.0 41.0 32.5 41.0 38-39 37.48154130748807 40.0 36.0 41.0 32.0 41.0 40-41 37.420267887317614 40.0 36.0 41.0 32.0 41.0 42-43 37.298432955578626 40.0 36.0 41.0 31.5 41.0 44-45 37.161737123017986 39.5 36.0 41.0 31.0 41.0 46-47 36.9632633500578 39.0 36.0 41.0 31.0 41.0 48-49 36.76594750557807 39.0 35.0 41.0 30.0 41.0 50-51 36.59444348129345 39.0 35.0 41.0 30.0 41.0 52-53 36.396788552194025 39.0 35.0 40.0 30.0 41.0 54-55 36.19520725510638 38.5 35.0 40.0 29.0 41.0 56-57 35.971018451244085 38.0 35.0 40.0 29.0 41.0 58-59 35.71354616885512 38.0 34.0 40.0 28.0 41.0 60-61 35.451345906654375 38.0 34.0 40.0 28.0 41.0 62-63 35.14841663748359 37.0 34.0 40.0 27.5 41.0 64-65 34.799800728399816 36.5 34.0 39.5 27.0 41.0 66-67 34.45104446678883 36.0 33.0 39.0 26.0 40.5 68-69 34.09553896376996 35.5 33.0 39.0 26.0 40.0 70-71 33.725493929370714 35.0 33.0 38.0 26.0 40.0 72-73 33.36908716664966 35.0 32.0 37.5 26.0 40.0 74-75 33.00061375040876 35.0 32.0 37.0 25.0 39.0 76-77 32.00019587350686 34.0 30.5 36.0 24.5 38.5 78-79 32.48887627711338 35.0 32.0 36.0 25.0 38.5 80-81 32.38500554283776 35.0 32.0 36.0 25.0 37.5 82-83 32.105200983127986 34.5 31.5 35.0 25.0 37.0 84-85 31.826398418066777 34.0 31.0 35.0 24.0 36.5 86-87 31.56764524775442 34.0 31.0 35.0 24.0 36.0 88-89 31.33681043366032 34.0 31.0 35.0 23.0 36.0 90-91 31.111096473721265 34.0 31.0 35.0 23.0 35.0 92-93 30.914221696922446 34.0 30.0 35.0 21.0 35.0 94-95 30.73409992278598 34.0 30.0 35.0 20.0 35.0 96-97 30.55455268409513 34.0 30.0 35.0 20.0 35.0 98-99 30.35080393492764 34.0 29.0 35.0 19.5 35.0 100 28.54376579116913 32.0 25.0 34.0 16.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.2755503709451723 1101 2 -0.28742484309799465 1101 3 -0.33201072241375584 1101 4 -0.16988300105727916 1101 5 -0.1758654290590087 1101 6 -0.2450564435911744 1101 7 -0.2572399071512308 1101 8 -0.31373641849486233 1101 9 -0.42464084060345186 1101 10-11 -0.3858261046493183 1101 12-13 -0.44594261932957124 1101 14-15 -0.5509174540950212 1101 16-17 -0.5750979117362149 1101 18-19 -0.5891883363412589 1101 20-21 -0.5996255560819037 1101 22-23 -0.6536594042029975 1101 24-25 -0.7469261647988503 1101 26-27 -0.7662467788826461 1101 28-29 -0.744981751802996 1101 30-31 -0.6863749085406923 1101 32-33 -0.5615628414331724 1101 34-35 -0.6712191659533318 1101 36-37 -0.6773628153400963 1101 38-39 -0.7262057894959355 1101 40-41 -0.69018924773912 1101 42-43 -0.6862371529487703 1101 44-45 -0.7859788937368677 1101 46-47 -0.8620653939432117 1101 48-49 -0.7827402280070075 1101 50-51 -0.7989512104057397 1101 52-53 -0.8101898772619549 1101 54-55 -0.7466681918247957 1101 56-57 -0.8275139043704911 1101 58-59 -0.8027266845016214 1101 60-61 -0.8455860213456958 1101 62-63 -0.8284620447107329 1101 64-65 -0.8293099426055477 1101 66-67 -0.8443110159701277 1101 68-69 -0.8432032679323669 1101 70-71 -0.9178663569286059 1101 72-73 -0.9385086123806232 1101 74-75 -0.9330550226801364 1101 76-77 -0.777711863928392 1101 78-79 -0.7464392432534908 1101 80-81 -0.7199675898074958 1101 82-83 -0.7478640905105571 1101 84-85 -0.8126718247440969 1101 86-87 -0.9070876774452259 1101 88-89 -1.0284234293807977 1101 90-91 -1.0719757467960598 1101 92-93 -1.159299499321584 1101 94-95 -1.3253055919353613 1101 96-97 -1.2709272709302617 1101 98-99 -1.3883225902772196 1101 100 -1.287167200320276 1102 1 -0.27496260497576586 1102 2 -0.26533266403334466 1102 3 -0.2800413325257445 1102 4 -0.16281182225986157 1102 5 -0.18705377382101318 1102 6 -0.25518172671849015 1102 7 -0.2344399382144573 1102 8 -0.3191858977790858 1102 9 -0.4884649087533788 1102 10-11 -0.4422647617740907 1102 12-13 -0.4748738114021194 1102 14-15 -0.5680755164550035 1102 16-17 -0.6726585869642676 1102 18-19 -0.6797579034432673 1102 20-21 -0.687650229924337 1102 22-23 -0.6842859117964366 1102 24-25 -0.7157508844782399 1102 26-27 -0.7436503276390525 1102 28-29 -0.7977776670593926 1102 30-31 -0.6695031629353849 1102 32-33 -0.7086157051539814 1102 34-35 -0.7971450186169307 1102 36-37 -0.7188401809945475 1102 38-39 -0.8449019767374679 1102 40-41 -0.8385498061112315 1102 42-43 -0.8431873150126208 1102 44-45 -0.8016366067871274 1102 46-47 -0.8830856314738185 1102 48-49 -0.886978246496497 1102 50-51 -0.9484676335711626 1102 52-53 -0.9182307536996817 1102 54-55 -0.9214473349597299 1102 56-57 -0.9297227584916072 1102 58-59 -0.9865610765766348 1102 60-61 -1.0600571019513865 1102 62-63 -1.0417475892804688 1102 64-65 -1.0063055490385082 1102 66-67 -1.1186834849777583 1102 68-69 -1.1074840913261497 1102 70-71 -1.1057665429189143 1102 72-73 -1.1415451489096426 1102 74-75 -1.0698691603489436 1102 76-77 -0.9149702112990425 1102 78-79 -0.8835834908647939 1102 80-81 -0.825188384525898 1102 82-83 -0.8414335730829912 1102 84-85 -0.9280662557259554 1102 86-87 -1.0160606401601058 1102 88-89 -1.0642132005552618 1102 90-91 -1.088713523393558 1102 92-93 -1.1275301373136806 1102 94-95 -1.1696088472433068 1102 96-97 -1.3378996865524009 1102 98-99 -1.2848566430365018 1102 100 -1.3029139523613011 1103 1 -0.3553209263663746 1103 2 -0.2849462128280109 1103 3 -0.3458951861848263 1103 4 -0.16719888599130428 1103 5 -0.18821274838695246 1103 6 -0.2960358173888338 1103 7 -0.29869317443002785 1103 8 -0.3546976076930193 1103 9 -0.4384328519832721 1103 10-11 -0.4821926443489204 1103 12-13 -0.5485359118085853 1103 14-15 -0.7569995683820352 1103 16-17 -0.7817420139117957 1103 18-19 -0.7254654431027276 1103 20-21 -0.7061978074555526 1103 22-23 -0.7765624315279638 1103 24-25 -0.7546878878425574 1103 26-27 -0.8582957769737334 1103 28-29 -0.8952281153104593 1103 30-31 -0.7638399340110382 1103 32-33 -0.7476849939704735 1103 34-35 -0.7724109055740698 1103 36-37 -0.7167655830166595 1103 38-39 -0.8317934822362645 1103 40-41 -0.9088901632659159 1103 42-43 -0.923348243181124 1103 44-45 -0.8288481613753476 1103 46-47 -0.9092398261377355 1103 48-49 -0.9779539375887936 1103 50-51 -0.9801552791142356 1103 52-53 -1.0311651622756415 1103 54-55 -0.999123520208542 1103 56-57 -0.9980575385383048 1103 58-59 -1.0696708236896981 1103 60-61 -1.0961592743021313 1103 62-63 -1.0418926552867092 1103 64-65 -1.043851898805201 1103 66-67 -1.1150808676378503 1103 68-69 -1.0714481771631341 1103 70-71 -0.9706164832176896 1103 72-73 -1.0903537690893756 1103 74-75 -1.1188580148912841 1103 76-77 -1.0536950935741451 1103 78-79 -0.8922175696545978 1103 80-81 -0.8812717439346685 1103 82-83 -0.8224821989009428 1103 84-85 -0.8760958331189634 1103 86-87 -1.004291446117044 1103 88-89 -0.9685851190782415 1103 90-91 -1.1123778276478653 1103 92-93 -1.0982609261193161 1103 94-95 -1.1781838138713212 1103 96-97 -1.3087516387051927 1103 98-99 -1.3643014865023844 1103 100 -1.4306424904374744 1104 1 -0.38758787731603306 1104 2 -0.3397609469437519 1104 3 -0.3437643004640698 1104 4 -0.1745812839309835 1104 5 -0.22802853597046635 1104 6 -0.34872007802088234 1104 7 -0.30931075227343996 1104 8 -0.3527723312706925 1104 9 -0.5014386234153392 1104 10-11 -0.5094319116397301 1104 12-13 -0.5284464882128361 1104 14-15 -0.6591886902671362 1104 16-17 -0.6739544491770388 1104 18-19 -0.677459507341645 1104 20-21 -0.6922012072635368 1104 22-23 -0.6905776325029862 1104 24-25 -0.7444934511385597 1104 26-27 -0.8209290817096715 1104 28-29 -0.8464602684564966 1104 30-31 -0.8087495288869135 1104 32-33 -0.7554477978071716 1104 34-35 -0.8145847699995841 1104 36-37 -0.7102347132745379 1104 38-39 -0.7920944709052264 1104 40-41 -0.9432242001882258 1104 42-43 -0.8302380436941093 1104 44-45 -0.9361318511706145 1104 46-47 -0.9338287000921355 1104 48-49 -0.905600969576497 1104 50-51 -0.8667123149169953 1104 52-53 -0.8422485631525305 1104 54-55 -0.8535606403229892 1104 56-57 -0.9078324771469113 1104 58-59 -0.9777696548909205 1104 60-61 -0.9540610489115622 1104 62-63 -1.03517096828201 1104 64-65 -0.9958936936297746 1104 66-67 -1.0517908223756791 1104 68-69 -0.8864398400322386 1104 70-71 -0.9618423608223097 1104 72-73 -0.9292839979014715 1104 74-75 -0.9968256953760672 1104 76-77 -0.8958806695738382 1104 78-79 -0.7949166692043654 1104 80-81 -0.8703291454563669 1104 82-83 -0.9115617141120929 1104 84-85 -0.8660586527306045 1104 86-87 -0.763956691675908 1104 88-89 -0.892549609074468 1104 90-91 -0.8532775974433697 1104 92-93 -0.9916440297765163 1104 94-95 -1.0123819168339487 1104 96-97 -1.1323315495103508 1104 98-99 -1.1275344943209369 1104 100 -1.1321438060961064 1105 1 -0.3980033840176809 1105 2 -0.37690513443650886 1105 3 -0.41835262795819617 1105 4 -0.22436242601642675 1105 5 -0.2927523309365654 1105 6 -0.32973223497390336 1105 7 -0.35156568843694913 1105 8 -0.3590552253192101 1105 9 -0.5601931656890571 1105 10-11 -0.5568467755006807 1105 12-13 -0.5437219507140156 1105 14-15 -0.7192960978508651 1105 16-17 -0.6570568621229924 1105 18-19 -0.7070037475174615 1105 20-21 -0.7687477883796348 1105 22-23 -0.7335390799463681 1105 24-25 -0.7549046301479052 1105 26-27 -0.7570971248539351 1105 28-29 -0.7723839193709381 1105 30-31 -0.8437435474422372 1105 32-33 -0.7370006138249252 1105 34-35 -0.7455810657276416 1105 36-37 -0.8628094908726425 1105 38-39 -0.8555115122003585 1105 40-41 -0.7929864850136923 1105 42-43 -0.800140743055735 1105 44-45 -0.8540528428344629 1105 46-47 -0.9704209672133146 1105 48-49 -0.8699688645896018 1105 50-51 -0.8817778300514405 1105 52-53 -0.9428098772533602 1105 54-55 -0.9482944840665226 1105 56-57 -0.9688731926810448 1105 58-59 -0.9838369127513786 1105 60-61 -1.0829381008639913 1105 62-63 -1.043935716308951 1105 64-65 -1.0429786213648313 1105 66-67 -1.0074149214420416 1105 68-69 -1.0455606439021636 1105 70-71 -1.0280544049637115 1105 72-73 -1.1229746095345376 1105 74-75 -1.102258482522057 1105 76-77 -1.052611850499158 1105 78-79 -1.0014386248205795 1105 80-81 -0.8543317067677094 1105 82-83 -0.8399631550071618 1105 84-85 -0.7820016369511755 1105 86-87 -0.8854787142239928 1105 88-89 -0.8761548913347035 1105 90-91 -0.8590332071679825 1105 92-93 -0.9476750172322781 1105 94-95 -0.9123060604538296 1105 96-97 -1.0038588808494389 1105 98-99 -1.1318990573211956 1105 100 -1.099987121112136 1106 1 -0.3644313174612037 1106 2 -0.32873465061416596 1106 3 -0.3705353998043215 1106 4 -0.20600638256915715 1106 5 -0.2262869895769981 1106 6 -0.32044319969857327 1106 7 -0.33102094723746234 1106 8 -0.36358561940312484 1106 9 -0.43805723250217454 1106 10-11 -0.4400484509534479 1106 12-13 -0.5207766597743415 1106 14-15 -0.6596372326243554 1106 16-17 -0.7105394998942174 1106 18-19 -0.6890384487851975 1106 20-21 -0.6325805738455443 1106 22-23 -0.6648866972487113 1106 24-25 -0.7335020354461435 1106 26-27 -0.662291010833286 1106 28-29 -0.7549084459627053 1106 30-31 -0.7226266469946836 1106 32-33 -0.7052355260724923 1106 34-35 -0.7314315028891372 1106 36-37 -0.7451496957555221 1106 38-39 -0.7524688281658243 1106 40-41 -0.7414823494208562 1106 42-43 -0.661692747665029 1106 44-45 -0.7373170563720777 1106 46-47 -0.7934518778618127 1106 48-49 -0.8125959184839786 1106 50-51 -0.836653491831143 1106 52-53 -0.8831866080286588 1106 54-55 -0.9010749509819505 1106 56-57 -0.8718087439055893 1106 58-59 -0.8930615304188834 1106 60-61 -1.0020539110529896 1106 62-63 -1.000318917781577 1106 64-65 -0.9844674821149653 1106 66-67 -0.8939237122347095 1106 68-69 -0.9426365117938786 1106 70-71 -0.8718351479256299 1106 72-73 -0.8940808582250455 1106 74-75 -0.95004392780708 1106 76-77 -0.9298085514360146 1106 78-79 -0.7989090032341366 1106 80-81 -0.7824411358409691 1106 82-83 -0.8120537261096885 1106 84-85 -0.7752834578330372 1106 86-87 -0.7628914250863588 1106 88-89 -0.8164298033470025 1106 90-91 -0.8099897592898948 1106 92-93 -0.781240890828137 1106 94-95 -0.8116794761697648 1106 96-97 -0.8973838024790801 1106 98-99 -1.0117049147666783 1106 100 -1.1078337915202674 1107 1 -0.38338273063663664 1107 2 -0.3787679507959325 1107 3 -0.4414064959665147 1107 4 -0.2744350690888524 1107 5 -0.28575127905389763 1107 6 -0.3144783620220153 1107 7 -0.38131052968693524 1107 8 -0.363580203128393 1107 9 -0.4760714227456546 1107 10-11 -0.4616420154150731 1107 12-13 -0.5227729533250667 1107 14-15 -0.6541364441930924 1107 16-17 -0.6621042979923359 1107 18-19 -0.7083910295773563 1107 20-21 -0.680528740360586 1107 22-23 -0.7437047894821944 1107 24-25 -0.7336464584302504 1107 26-27 -0.7010082929836159 1107 28-29 -0.7687814740836671 1107 30-31 -0.7332638472773567 1107 32-33 -0.7497520201812762 1107 34-35 -0.7540652232813514 1107 36-37 -0.7862782439686598 1107 38-39 -0.7500067019622563 1107 40-41 -0.7744641929450751 1107 42-43 -0.7608969383820963 1107 44-45 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1.0557417878542665 2311 34-35 0.989694075910549 2311 36-37 1.0644076335895178 2311 38-39 1.111671837768263 2311 40-41 1.0859773854642398 2311 42-43 1.1799501821639993 2311 44-45 1.166929655731579 2311 46-47 1.2138037631979302 2311 48-49 1.2327959060326492 2311 50-51 1.2380011932902377 2311 52-53 1.1857565803011525 2311 54-55 1.1778567087454235 2311 56-57 1.248368327898163 2311 58-59 1.2588666151290298 2311 60-61 1.2665749362711551 2311 62-63 1.3177454784476694 2311 64-65 1.3395969465075837 2311 66-67 1.406153000224677 2311 68-69 1.3495966417611953 2311 70-71 1.330592871386692 2311 72-73 1.3435961946481072 2311 74-75 1.2432234807169422 2311 76-77 1.1653128398415014 2311 78-79 1.0711938511539003 2311 80-81 1.1135630374796506 2311 82-83 1.1006413148525809 2311 84-85 1.159358463381988 2311 86-87 1.2857257644415938 2311 88-89 1.2562601822123405 2311 90-91 1.3232102618387778 2311 92-93 1.3624844888705638 2311 94-95 1.4245580897287553 2311 96-97 1.4829733220717145 2311 98-99 1.5107482273557515 2311 100 1.5894638068080127 2312 1 0.18591919653825073 2312 2 0.15564663358949105 2312 3 0.34356541279959174 2312 4 0.2529878762468485 2312 5 0.3347807484953691 2312 6 0.41192755902129363 2312 7 0.46826458961809436 2312 8 0.2753346767128164 2312 9 0.4277819101946676 2312 10-11 0.2834165871305956 2312 12-13 0.43883191557636536 2312 14-15 0.7988953628853324 2312 16-17 0.888144456383877 2312 18-19 0.9249614349487914 2312 20-21 0.9167544210637999 2312 22-23 0.5497729381450114 2312 24-25 0.5180433221364922 2312 26-27 0.8312829467699814 2312 28-29 0.977599130408862 2312 30-31 0.9388216693088296 2312 32-33 0.7545818971704321 2312 34-35 0.9609030240313174 2312 36-37 0.9954794546474446 2312 38-39 1.0192069048444168 2312 40-41 1.027611708660615 2312 42-43 0.9137399611979689 2312 44-45 0.821361655647614 2312 46-47 1.005152365757752 2312 48-49 1.0805604939026168 2312 50-51 1.0838809711689592 2312 52-53 1.1139673754054584 2312 54-55 1.1032367390640019 2312 56-57 1.0871016296207117 2312 58-59 1.1065523238035055 2312 60-61 1.156338666807038 2312 62-63 1.1815017121396565 2312 64-65 1.1608733269258593 2312 66-67 1.1554883751076872 2312 68-69 1.1475218988348104 2312 70-71 1.0040569293830686 2312 72-73 1.1205118679632804 2312 74-75 1.2133586544164672 2312 76-77 1.1115631732886264 2312 78-79 1.03143915083173 2312 80-81 0.9247397374407242 2312 82-83 0.9240523076655762 2312 84-85 0.7379239063435357 2312 86-87 1.0024399182601513 2312 88-89 0.6952460442332047 2312 90-91 1.0142386799278107 2312 92-93 1.1885482729196397 2312 94-95 1.3134252917458404 2312 96-97 1.390000753354716 2312 98-99 1.357787530079694 2312 100 1.5235097436280576 2313 1 -0.34573619918639764 2313 2 -0.002527753666015542 2313 3 0.2579164278111108 2313 4 0.10984903566238557 2313 5 0.24282503633077823 2313 6 0.35552250624347437 2313 7 0.14357060932412935 2313 8 0.10516009069120003 2313 9 0.27060443293463976 2313 10-11 0.37798730408275816 2313 12-13 0.5672388663724917 2313 14-15 0.6286352580561498 2313 16-17 0.7813866853268578 2313 18-19 0.6515843079550265 2313 20-21 0.8703507887609732 2313 22-23 0.9120617540688869 2313 24-25 0.7610619371688969 2313 26-27 0.9750778633904318 2313 28-29 0.8575559434110431 2313 30-31 0.8499952278702736 2313 32-33 0.9760593433688385 2313 34-35 1.0055452252919395 2313 36-37 0.917525670101746 2313 38-39 0.690642744053811 2313 40-41 0.8067591439395088 2313 42-43 0.8525708637500813 2313 44-45 1.0309271825279183 2313 46-47 1.0653276801272114 2313 48-49 1.1204944601972215 2313 50-51 1.0288456098067584 2313 52-53 0.969845658646328 2313 54-55 1.0433842297049196 2313 56-57 1.1004078215852786 2313 58-59 1.0446359881389853 2313 60-61 1.0673416150760673 2313 62-63 1.1153065262326436 2313 64-65 1.1781436086423653 2313 66-67 1.1803590730175344 2313 68-69 1.1870749510117662 2313 70-71 1.09099751425547 2313 72-73 1.138916332012478 2313 74-75 0.812023604629502 2313 76-77 0.9902212575786216 2313 78-79 0.8001560657939208 2313 80-81 0.9481295857640859 2313 82-83 0.9621071663798659 2313 84-85 0.9910608247896953 2313 86-87 1.04626699803746 2313 88-89 1.055710483799654 2313 90-91 1.1868062053625685 2313 92-93 0.9859772457447491 2313 94-95 0.9726865777357219 2313 96-97 1.2832965604275408 2313 98-99 1.389690393140576 2313 100 1.423786918711226 2314 1 -0.3655762972476708 2314 2 -0.14846897255530678 2314 3 0.10066378564903289 2314 4 -0.13594541856259923 2314 5 0.15701699907538824 2314 6 0.18978474197842843 2314 7 0.10438109627726533 2314 8 0.11284962937624243 2314 9 0.18596145367463635 2314 10-11 0.38480097865987517 2314 12-13 0.507002904456364 2314 14-15 0.5543530030465291 2314 16-17 0.7617518076098904 2314 18-19 0.8035628393154823 2314 20-21 0.4593713514100273 2314 22-23 0.6659066507123157 2314 24-25 0.8727904329448108 2314 26-27 0.8018297911194878 2314 28-29 0.7516934964367792 2314 30-31 0.7540846957120166 2314 32-33 0.7973048214768994 2314 34-35 0.8418603539226623 2314 36-37 0.769964615759406 2314 38-39 0.8186399343130262 2314 40-41 0.8362419169777908 2314 42-43 0.6824392350972559 2314 44-45 0.6905460580914848 2314 46-47 0.8464184721643662 2314 48-49 0.7098333787828182 2314 50-51 0.8567022008216654 2314 52-53 0.9296321347673597 2314 54-55 0.9658127713402465 2314 56-57 0.8403660244357383 2314 58-59 0.8238452455806637 2314 60-61 0.928374722268444 2314 62-63 0.6241581983716529 2314 64-65 0.7424728470634321 2314 66-67 0.9395860789558768 2314 68-69 0.9894570467834853 2314 70-71 1.0428781955968347 2314 72-73 1.057329933395856 2314 74-75 1.030628606209092 2314 76-77 0.8106869398356302 2314 78-79 0.8014125416197544 2314 80-81 0.8136647135554824 2314 82-83 0.7882908543575553 2314 84-85 0.7153156663916853 2314 86-87 0.8879576568714356 2314 88-89 0.9540780164906373 2314 90-91 1.0708464839496052 2314 92-93 1.1455449508457747 2314 94-95 1.1879967116327208 2314 96-97 1.2567147094277722 2314 98-99 1.2420661637465393 2314 100 1.277196005508383 2315 1 -0.11218752634666274 2315 2 0.07960235299779583 2315 3 0.11964593235100551 2315 4 -0.08140074731011993 2315 5 0.24793012303651807 2315 6 0.3674775390411824 2315 7 0.4239813850662486 2315 8 0.38043320625115484 2315 9 0.5746367488935107 2315 10-11 0.5595273061762001 2315 12-13 0.6267689944330073 2315 14-15 0.7353078677577329 2315 16-17 0.7620170282803045 2315 18-19 0.8160399861275422 2315 20-21 0.7856720768684369 2315 22-23 0.8824181242552953 2315 24-25 0.8186251902787234 2315 26-27 0.9038725971825485 2315 28-29 0.9728423645099866 2315 30-31 0.897635727394146 2315 32-33 0.9476574961173512 2315 34-35 0.9566497310829334 2315 36-37 0.9773234663108994 2315 38-39 0.9776194325002265 2315 40-41 0.9727881245121921 2315 42-43 1.0320675049336003 2315 44-45 0.9198412810808065 2315 46-47 0.9147671082170348 2315 48-49 1.02492863185077 2315 50-51 0.9568062833589153 2315 52-53 0.9582196663693381 2315 54-55 0.8761599783020841 2315 56-57 1.0082350753293525 2315 58-59 1.0357280232907655 2315 60-61 1.1079447107460467 2315 62-63 1.0812213007691227 2315 64-65 1.0895017112239742 2315 66-67 1.112478905015145 2315 68-69 1.03842791056897 2315 70-71 0.6622818743940613 2315 72-73 0.9039718296671424 2315 74-75 1.1015875272465934 2315 76-77 1.0283882004351348 2315 78-79 0.9260328026740368 2315 80-81 0.9498516232193808 2315 82-83 0.9363976004709116 2315 84-85 0.8969188463780959 2315 86-87 0.8492020972239089 2315 88-89 0.9931662036168554 2315 90-91 1.0757779976521853 2315 92-93 1.1377798575252491 2315 94-95 1.1830041515990004 2315 96-97 1.2661575955793047 2315 98-99 1.1281576543785086 2315 100 1.1367963225373643 2316 1 0.5039801002670288 2316 2 0.5010599584341549 2316 3 0.49394402885370425 2316 4 0.3034686239885147 2316 5 0.4035983069192284 2316 6 0.4195358423327349 2316 7 0.49738045566840583 2316 8 0.4986926128893927 2316 9 0.558468877385387 2316 10-11 0.5094048031894047 2316 12-13 0.654819642660172 2316 14-15 0.7867985359548655 2316 16-17 0.807568207727094 2316 18-19 0.8096113584954665 2316 20-21 0.8648883252917585 2316 22-23 0.9115879505073892 2316 24-25 0.9616948242819774 2316 26-27 1.0040626423883836 2316 28-29 0.9558852724796978 2316 30-31 0.806810317262233 2316 32-33 0.8098206291530943 2316 34-35 0.746513698685348 2316 36-37 0.6830645578650874 2316 38-39 0.6446784509338315 2316 40-41 0.7857699605917432 2316 42-43 0.8999435113113208 2316 44-45 0.798313879893449 2316 46-47 0.8526218466038671 2316 48-49 0.597511721762956 2316 50-51 0.6723237911635849 2316 52-53 0.7986014779191777 2316 54-55 0.9097302576189463 2316 56-57 0.9457765123893225 2316 58-59 0.8432561841372888 2316 60-61 0.9063859925094064 2316 62-63 0.8939733910688759 2316 64-65 0.9076733907230548 2316 66-67 0.9063930071367281 2316 68-69 0.8954744714242509 2316 70-71 0.7376778535898367 2316 72-73 0.8100624585369189 2316 74-75 0.8324403263043791 2316 76-77 0.7221808990788183 2316 78-79 0.7106105185457068 2316 80-81 0.6526237556121188 2316 82-83 0.6161174889647754 2316 84-85 0.6806082349649323 2316 86-87 0.6963253596853605 2316 88-89 0.6860982157570454 2316 90-91 0.769719113222795 2316 92-93 0.8231774304637831 2316 94-95 0.7219116512996777 2316 96-97 0.9103050753824746 2316 98-99 0.992056254431116 2316 100 0.704711594633693 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 0.0 11 12.0 12 67.0 13 248.0 14 1210.0 15 3474.0 16 8328.0 17 16966.0 18 28206.0 19 39966.0 20 53486.0 21 70510.0 22 86360.0 23 94734.0 24 101504.0 25 113277.0 26 134989.0 27 165889.0 28 203257.0 29 249596.0 30 306159.0 31 379815.0 32 478427.0 33 624274.0 34 875341.0 35 1174917.0 36 1768918.0 37 2701044.0 38 2374289.0 39 363417.0 40 48.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.19308092298294 19.42886661708724 12.844253334586917 24.53379912534289 2 15.730184626784272 20.663048529362385 40.27230967033744 23.334457173515904 3 17.372117337621052 27.591408717543374 30.95611724485793 24.08035669997765 4 12.08068555435359 17.146050769061272 38.15805750579703 32.615206170788106 5 12.439078578778929 37.958992776035856 34.35565926312812 15.246269382057104 6 29.72282668563157 37.18780216460172 19.54987660572001 13.5394945440467 7 26.972181490670128 32.974866149543715 23.197675120845883 16.85527723894028 8 26.703651822464806 35.379568643227344 20.49848148063449 17.418298053673354 9 28.094678981764982 15.001188954934783 20.130516893829668 36.77361516947057 10-11 25.301913578159247 25.67485356666039 29.106442583797552 19.916790271382812 12-13 25.32249472498773 23.751632770650595 28.63194967038194 22.29392283397973 14-15 23.10475016135915 25.107298651540372 26.38681400921536 25.401137177885126 16-17 22.568935468046778 27.313862399301186 27.089555796267504 23.027646336384535 18-19 22.238358691940213 27.018207740904888 28.59430300798093 22.149130559173972 20-21 23.303557868979475 26.527419157166364 27.844542990963806 22.324479982890356 22-23 22.891051205443453 26.365529289089455 27.96142133230467 22.781998173162414 24-25 22.307157166472987 26.125505441563 28.387324604012143 23.180012787951867 26-27 22.45264349558534 26.321590771743352 28.04405797792318 23.181707754748132 28-29 22.428955225166582 27.278147297586695 27.956005810480406 22.336891666766316 30-31 23.57513579203876 26.372817370558504 28.0110466362532 22.041000201149533 32-33 23.469146643842038 26.32921440902453 27.754458485772243 22.447180461361185 34-35 23.233663156369527 26.65369298022322 28.033709677360896 22.078934186046354 36-37 22.87194079173777 26.70609437898554 27.966143095225505 22.45582173405118 38-39 22.909484192368403 26.743518034235546 27.894230755291183 22.45276701810487 40-41 22.926728181641725 26.105822339606032 28.40010240998854 22.567347068763702 42-43 22.727086055748405 26.469516305823532 27.80188650681708 23.00151113161098 44-45 22.051260348991843 26.81536918982624 28.27585382139744 22.85751663978448 46-47 22.056402786640597 26.157257699021407 27.984930038621197 23.8014094757168 48-49 22.425156274265447 26.298540307424112 28.044873440282192 23.231429978028252 50-51 22.24496900836513 26.655987014333054 27.833896240455818 23.265147736846 52-53 21.98307649771916 26.848476692311973 27.576967530092745 23.591479279876122 54-55 21.82725392643057 26.485992871098123 28.125274688371764 23.56147851409954 56-57 22.349922392915307 26.369305039125646 27.639081760221284 23.64169080773776 58-59 21.851783058303724 26.553087609013037 28.148919566456765 23.446209766226474 60-61 22.441360456965942 26.816189869991337 27.55843219140834 23.184017481634385 62-63 22.4967294014699 26.68053887079126 27.28098504835308 23.54174667938576 64-65 21.970336740060027 26.30922889594406 27.430729288755312 24.2897050752406 66-67 21.78191998542821 26.302384837412536 27.35974582830384 24.555949348855414 68-69 21.422352968996343 26.446689328205466 27.636208622317902 24.49474908048029 70-71 21.738192049986495 26.22708654964664 27.76487067651158 24.269850723855285 72-73 21.716100312479607 26.77848345485318 27.177178918944612 24.3282373137226 74-75 21.904948198896452 26.763643062233257 26.917544570584024 24.41386416828627 76-77 21.734247164441474 26.52483675368908 27.04727488799187 24.693641193877582 78-79 21.530912533808653 26.510596435639865 27.338263970717534 24.620227059833947 80-81 21.608271620108834 26.778807230718265 26.98603901191629 24.62688213725661 82-83 21.971046330647347 26.617993187973642 27.083324187993497 24.32763629338551 84-85 22.138797391011767 26.84130199023106 26.508703240297066 24.51119737846011 86-87 21.573560528133974 26.928079900694517 26.796443583814135 24.70191598735737 88-89 21.58163783268124 26.49345595297181 26.90561730941531 25.019288904931642 90-91 22.159524562685135 26.245171007493834 26.898608939227454 24.69669549059358 92-93 22.020986452005396 26.54278258764176 26.680595936817852 24.755635023534992 94-95 21.45468671873358 26.3552044377805 26.86674281042628 25.323366033059642 96-97 21.292870490697823 26.253350505875456 27.104044410680583 25.34973459274614 98-99 21.392151194843354 26.44902588833309 26.81063651927891 25.348186397544648 100 21.294001472725938 26.439940752701 26.963029875493206 25.303027899079854 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 814.0 1 632.5 2 1309.5 3 2071.0 4 1555.0 5 1292.5 6 1764.5 7 3155.0 8 4906.0 9 5905.0 10 5804.0 11 5602.5 12 6358.0 13 7458.0 14 8685.5 15 9966.5 16 11038.0 17 12422.0 18 13932.5 19 15123.0 20 16616.5 21 18776.0 22 21912.5 23 26670.5 24 33533.0 25 41836.0 26 52392.5 27 65568.5 28 81034.0 29 99914.5 30 121416.0 31 142432.5 32 168720.5 33 195041.0 34 218316.5 35 244093.0 36 270854.0 37 306833.0 38 330382.5 39 332035.0 40 336167.0 41 344095.0 42 355637.5 43 372793.5 44 393779.5 45 426716.0 46 460848.0 47 490178.0 48 525187.0 49 551095.0 50 566229.0 51 574911.0 52 560280.0 53 528512.5 54 493136.0 55 444594.5 56 396055.5 57 348551.5 58 297722.5 59 250599.5 60 198172.5 61 150518.5 62 116830.0 63 90237.0 64 65975.5 65 46304.5 66 32864.5 67 24882.0 68 18612.5 69 13437.0 70 10035.5 71 7472.5 72 5595.0 73 4145.0 74 3036.5 75 2070.0 76 1368.5 77 859.5 78 521.0 79 317.5 80 214.0 81 142.5 82 86.0 83 53.5 84 38.0 85 28.5 86 20.0 87 11.0 88 5.5 89 3.5 90 4.5 91 5.5 92 4.5 93 1.5 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05090698089957515 2 0.0 3 8.05235382783536E-6 4 0.01637043533198929 5 0.0038490251297053023 6 8.05235382783536E-6 7 1.5299472272887183E-4 8 4.2677475287527416E-4 9 0.0020936119952371934 10-11 8.777065672340543E-4 12-13 3.01963268543826E-4 14-15 2.8183238397423765E-5 16-17 1.046805997618597E-4 18-19 0.0 20-21 2.4157061483506084E-5 22-23 4.02617691391768E-6 24-25 6.844500753660056E-5 26-27 1.610470765567072E-5 28-29 0.001083041589843856 30-31 8.616018595783837E-4 32-33 0.01395472918363868 34-35 0.02277608280203232 36-37 0.030377504815508896 38-39 0.02021946046169459 40-41 1.4896854581495416E-4 42-43 0.0017795701959516146 44-45 0.010270777307404002 46-47 0.014836461927786652 48-49 0.005938610948028578 50-51 0.010838468252266396 52-53 0.016362382978161453 54-55 0.010053363754052448 56-57 0.003253150946445486 58-59 0.008096641773888455 60-61 0.0016950204807593433 62-63 0.0010508321745325146 64-65 0.019812816593388904 66-67 0.045918547703231144 68-69 0.05596788528036968 70-71 0.049087148934484355 72-73 0.041219999244689214 74-75 0.029358882056287727 76-77 0.02955616472506969 78-79 0.01292805407058967 80-81 0.0021499784720320414 82-83 0.028026217497780972 84-85 0.048511405635794126 86-87 0.04928845778018025 88-89 0.0599819836635456 90-91 0.035349833304197234 92-93 0.027265270061050527 94-95 0.007971830289557008 96-97 0.0036638209916650894 98-99 0.02214397302654724 100 0.01850430909636566 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.2418729E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.021729085549374 #Duplication Level Percentage of deduplicated Percentage of total 1 73.15093279899726 41.711926724196495 2 15.373195165739837 17.532123398401886 3 5.222931113633993 8.934616889823726 4 2.3090712832517024 5.266689486112017 5 1.2025796970840696 3.4286586845454927 6 0.7087338544317943 2.4247937904700176 7 0.445868631398901 1.7796940215160966 8 0.3000142062958554 1.3685863034574712 9 0.2157171430911508 1.1070508043207063 >10 1.0034412504925996 9.815284338551972 >50 0.04509550534395614 1.7401982702231034 >100 0.01929546618430343 1.989320874607016 >500 0.00188619937394383 0.7397911945750258 >1k 0.0011268474182723862 1.187332822721254 >5k 5.5418631283803366E-5 0.24829693326600968 >10k+ 5.5418631283803366E-5 0.7256354632118137 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 29044 0.2338725645756502 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22582 0.18183825414017812 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17262 0.138999731776094 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.052353827835361E-6 2 0.0 8.052353827835361E-6 0.0 8.052353827835361E-6 8.052353827835361E-6 3 0.0 8.052353827835361E-6 0.0 8.052353827835361E-6 8.052353827835361E-6 4 0.0 8.052353827835361E-6 0.0 1.6104707655670723E-5 1.6104707655670723E-5 5 0.0 2.415706148350608E-5 0.0 1.6104707655670723E-5 1.6104707655670723E-5 6 0.0 2.415706148350608E-5 0.0 2.415706148350608E-5 1.6104707655670723E-5 7 0.0 3.2209415311341445E-5 0.0 2.415706148350608E-5 1.6104707655670723E-5 8 0.0 3.2209415311341445E-5 0.0 3.2209415311341445E-5 1.6104707655670723E-5 9 0.0 3.2209415311341445E-5 0.0 6.441883062268289E-5 1.6104707655670723E-5 10-11 0.0 3.2209415311341445E-5 0.0 8.05235382783536E-5 2.01308845695884E-5 12-13 0.0 3.2209415311341445E-5 0.0 1.0870677667577737E-4 6.441883062268289E-5 14-15 0.0 4.4287946053094484E-5 0.0 1.1273295358969505E-4 9.260206902010664E-5 16-17 0.0 5.636647679484752E-5 0.0 1.5299472272887186E-4 1.0870677667577737E-4 18-19 0.0 5.636647679484752E-5 0.0 2.4157061483506083E-4 1.248114843314481E-4 20-21 0.0 6.441883062268289E-5 0.0 4.871674065840393E-4 1.2883766124536578E-4 22-23 0.0 6.441883062268289E-5 0.0 0.0011756436588639626 1.4896854581495416E-4 24-25 0.0 6.441883062268289E-5 0.0 0.002745852655291858 1.610470765567072E-4 26-27 0.0 6.441883062268289E-5 0.0 0.004887778773496064 1.6909943038454257E-4 28-29 0.0 6.441883062268289E-5 0.0 0.013097153500974214 1.6909943038454257E-4 30-31 0.0 6.441883062268289E-5 0.0 0.047122374600492525 1.7715178421237794E-4 32-33 0.0 6.441883062268289E-5 0.0 0.12364791920332588 1.852041380402133E-4 34-35 0.0 8.05235382783536E-5 0.0 0.22196715944119563 2.01308845695884E-4 36-37 0.0 1.0870677667577737E-4 0.0 0.3740036520645551 2.0936119952371938E-4 38-39 0.0 1.5299472272887186E-4 0.0 0.676655396860661 2.1741355335155475E-4 40-41 0.0 1.7715178421237794E-4 0.0 1.0799978000969341 2.3754443792114316E-4 42-43 0.0 1.7715178421237794E-4 0.0 1.442619450025844 2.4157061483506083E-4 44-45 4.026176913917681E-6 1.7715178421237794E-4 0.0 1.8085385388472524 2.4157061483506083E-4 46-47 8.052353827835361E-6 1.7715178421237794E-4 0.0 2.219989662388156 2.5767532249073156E-4 48-49 8.052353827835361E-6 1.811779611262956E-4 0.0 2.6789174640979763 2.8183238397423764E-4 50-51 1.2078530741753042E-5 1.852041380402133E-4 0.0 3.199192123445161 2.979370916299083E-4 52-53 1.6104707655670723E-5 1.8923031495413097E-4 0.0 3.7346615744654708 3.3417268385516747E-4 54-55 1.6104707655670723E-5 2.1338737643763705E-4 0.0 4.283183891040702 3.7040827608042655E-4 56-57 1.6104707655670723E-5 2.3351826100722546E-4 0.0 4.83387229079562 3.8651298373609734E-4 58-59 1.6104707655670723E-5 2.3351826100722546E-4 0.0 5.487522112770156 3.945653375639327E-4 60-61 1.6104707655670723E-5 2.3351826100722546E-4 0.0 6.143954022992208 4.1067004521960337E-4 62-63 1.6104707655670723E-5 2.3351826100722546E-4 0.0 6.915208472622279 4.509318143587802E-4 64-65 1.6104707655670723E-5 2.3351826100722546E-4 0.0 7.556155706433404 4.509318143587802E-4 66-67 1.6104707655670723E-5 2.4962296866289617E-4 0.0 8.216440667962075 4.6301034510053325E-4 68-69 1.6104707655670723E-5 2.4962296866289617E-4 0.0 8.9741430060999 4.7508887584228626E-4 70-71 1.6104707655670723E-5 2.617014994046492E-4 0.0 9.758925410160735 4.7508887584228626E-4 72-73 1.6104707655670723E-5 2.7378003014640225E-4 0.0 10.46489540113163 5.435338833788869E-4 74-75 1.6104707655670723E-5 2.7378003014640225E-4 0.0 11.140874400270754 5.67690944862393E-4 76-77 1.6104707655670723E-5 2.7378003014640225E-4 0.0 11.85092693463236 5.878218294319813E-4 78-79 1.6104707655670723E-5 2.8183238397423764E-4 0.0 12.605211048570268 5.999003601737344E-4 80-81 1.6104707655670723E-5 2.89884737802073E-4 0.0 13.345125737102403 6.200312447433228E-4 82-83 1.6104707655670723E-5 2.89884737802073E-4 0.0 14.122596603887564 6.683453677103349E-4 84-85 1.6104707655670723E-5 2.89884737802073E-4 0.0 14.882098643105909 6.723715446242525E-4 86-87 1.6104707655670723E-5 2.89884737802073E-4 0.0 15.6084330369074 6.965286061077587E-4 88 1.6104707655670723E-5 2.89884737802073E-4 0.0 16.158255808625828 7.247118445051824E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 14680 0.0 55.351326 2 GGTATCA 17525 0.0 48.07945 1 ACCTGGG 16680 0.0 48.010677 3 GTATCAA 30250 0.0 39.94728 1 CCTGGGG 15795 0.0 37.37157 4 GTACCTG 23920 0.0 34.45881 1 CTGGGGG 11240 0.0 33.19316 5 TGGGGGG 27205 0.0 32.695465 6 GTACATG 44725 0.0 30.971476 1 CAACGCA 38645 0.0 30.349087 5 ATCAACG 38790 0.0 30.228397 3 TACATGG 45170 0.0 30.14099 2 TATCAAC 40200 0.0 29.635414 2 TAACGCA 1540 0.0 29.29691 4 TCAACGC 40255 0.0 29.233837 4 ATAACGC 1510 0.0 28.944786 3 ACATGGG 44870 0.0 28.928902 3 AACGCAG 41635 0.0 28.789724 6 ATGGGGG 26765 0.0 28.704165 5 CATGGGG 35590 0.0 28.232609 4 >>END_MODULE