FastQCFastQC Report
Mon 6 Jun 2016
ERR1211427_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211427_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9212669
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT303690.32964388495885394No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT222910.24196028317092474No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147540.1601490295591864No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA142750.051.851231
TACCTGG95550.046.2020232
GTATCAA265250.043.2161141
ACCTGGG113550.037.6310583
ATCAACG325550.034.6259043
CAACGCA328050.034.3329965
TATCAAC335900.033.865342
TCAACGC340550.033.1554344
AACGCAG349700.032.6917086
GTACCTG146000.030.6892781
GTACATG328700.029.642161
CCTGGGG110750.029.5411974
ACGCAGA382550.029.417497
TACATGG325950.029.2390232
CGCAGAG390200.028.730928
ACATGGG332400.027.6333313
CATGGGG261600.026.4504244
TGGGGGG219000.026.0367246
ATGGGGG197950.025.979265
GCAGAGT421400.025.6888989
Produced by FastQC (version 0.11.5)