FastQCFastQC Report
Mon 6 Jun 2016
ERR1211427_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211427_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9212669
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT271180.29435552281320426No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT212380.23053037073186936No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142040.15417898982368736No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA138650.051.9491271
TACCTGG98200.048.2381132
ACCTGGG110300.042.436013
GTATCAA253150.041.8429831
ATCAACG317600.032.701113
CAACGCA320200.032.487765
TATCAAC329650.031.904232
CCTGGGG103450.031.4816994
TCAACGC333100.031.4055184
GTACCTG153550.031.2618941
AACGCAG341200.031.023936
GTACATG325850.027.6883181
ACGCAGA383600.027.276357
TACATGG325200.027.1963332
TGGGGGG189000.026.6985136
CGCAGAG391650.026.5597558
ACATGGG324200.026.193643
CTGGGGG74850.025.799635
ATGGGGG181850.025.2432175
CATGGGG249250.024.6619834
Produced by FastQC (version 0.11.5)