##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211427_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9212669 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.084740372198326 33.0 31.0 34.0 30.0 34.0 2 32.21460284744844 33.0 31.0 34.0 30.0 34.0 3 32.29552554205519 34.0 31.0 34.0 30.0 34.0 4 35.93987366744643 37.0 35.0 37.0 35.0 37.0 5 35.79985691442947 37.0 35.0 37.0 35.0 37.0 6 35.83501556389359 37.0 35.0 37.0 35.0 37.0 7 35.765628614248484 37.0 35.0 37.0 33.0 37.0 8 35.74754243314288 37.0 35.0 37.0 33.0 37.0 9 37.3385621474081 39.0 37.0 39.0 34.0 39.0 10-11 37.31148514073392 39.0 37.0 39.0 34.0 39.0 12-13 37.22616632595831 39.0 37.0 39.0 33.0 39.0 14-15 38.436183640159 40.0 38.0 41.0 34.0 41.0 16-17 38.42475307644288 40.0 38.0 41.0 34.0 41.0 18-19 38.43040936345374 40.0 38.0 41.0 34.0 41.0 20-21 38.3899417204721 40.0 38.0 41.0 34.0 41.0 22-23 38.29065816865884 40.0 38.0 41.0 34.0 41.0 24-25 38.175580388267505 40.0 37.5 41.0 33.0 41.0 26-27 38.05546107213881 40.0 37.0 41.0 33.0 41.0 28-29 37.982178508747026 40.0 37.0 41.0 33.0 41.0 30-31 38.02716975938243 40.0 37.0 41.0 33.0 41.0 32-33 37.87342500853987 40.0 37.0 41.0 33.0 41.0 34-35 37.77108778140189 40.0 37.0 41.0 33.0 41.0 36-37 37.63668275719013 40.0 37.0 41.0 33.0 41.0 38-39 37.52670344500601 40.0 36.5 41.0 32.0 41.0 40-41 37.46318895208327 40.0 36.0 41.0 32.0 41.0 42-43 37.33793257958145 40.0 36.0 41.0 32.0 41.0 44-45 37.19461873643783 40.0 36.0 41.0 31.0 41.0 46-47 36.98243147561255 39.0 36.0 41.0 31.0 41.0 48-49 36.77578392320402 39.0 35.5 41.0 30.5 41.0 50-51 36.60139439504448 39.0 35.0 41.0 30.0 41.0 52-53 36.40208195909351 39.0 35.0 40.0 30.0 41.0 54-55 36.20570917070829 39.0 35.0 40.0 29.0 41.0 56-57 35.98300226568435 38.0 35.0 40.0 29.0 41.0 58-59 35.72579933133384 38.0 34.5 40.0 28.0 41.0 60-61 35.47097865993015 38.0 34.0 40.0 28.0 41.0 62-63 35.1731036358736 37.0 34.0 40.0 27.5 41.0 64-65 34.82502573358491 37.0 34.0 39.5 27.0 41.0 66-67 34.47724448799799 36.0 33.5 39.0 26.0 41.0 68-69 34.11462058389377 36.0 33.0 39.0 26.0 40.0 70-71 33.74298859537882 35.0 33.0 38.5 26.0 40.0 72-73 33.382640416148675 35.0 32.5 37.5 25.5 40.0 74-75 33.01491733828709 35.0 32.0 37.0 25.0 39.0 76-77 31.997535893235714 34.0 30.5 36.0 24.5 38.5 78-79 32.48323650833434 35.0 32.0 36.0 25.0 38.5 80-81 32.379108757733505 35.0 32.0 36.0 25.0 37.5 82-83 32.10467167549382 34.5 31.5 35.0 25.0 37.0 84-85 31.82834160220019 34.0 31.0 35.0 24.0 36.5 86-87 31.575230858722918 34.0 31.0 35.0 24.0 36.0 88-89 31.349859253599583 34.0 31.0 35.0 23.0 36.0 90-91 31.13258627874289 34.0 31.0 35.0 23.0 35.0 92-93 30.95040720555574 34.0 30.5 35.0 22.0 35.0 94-95 30.779966478769616 34.0 30.0 35.0 20.0 35.0 96-97 30.607050899147687 34.0 30.0 35.0 20.0 35.0 98-99 30.42042615446186 34.0 30.0 35.0 19.5 35.0 100 28.611377983947975 32.0 26.0 34.0 17.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.2192015928219888 1101 2 -0.2150962409566901 1101 3 -0.24705393573625045 1101 4 -0.13160017135749058 1101 5 -0.1315404693107567 1101 6 -0.20927156083112664 1101 7 -0.21802623184299108 1101 8 -0.2830812183615663 1101 9 -0.40242096340457323 1101 10-11 -0.3553257364455433 1101 12-13 -0.3791002603618878 1101 14-15 -0.5369564590115985 1101 16-17 -0.5523812690559922 1101 18-19 -0.5751698683232931 1101 20-21 -0.5711723062175835 1101 22-23 -0.6076852748346369 1101 24-25 -0.6976405267758778 1101 26-27 -0.6170601288510937 1101 28-29 -0.6370053059639531 1101 30-31 -0.6298553937629876 1101 32-33 -0.4624569505570477 1101 34-35 -0.5484455695962183 1101 36-37 -0.5833020435196659 1101 38-39 -0.6635595438609769 1101 40-41 -0.6626315774266871 1101 42-43 -0.6288106395492008 1101 44-45 -0.6831942258127413 1101 46-47 -0.7773082121660124 1101 48-49 -0.7237064387971088 1101 50-51 -0.7033890203487445 1101 52-53 -0.645322107444315 1101 54-55 -0.6624003384569619 1101 56-57 -0.7236516749958071 1101 58-59 -0.6662947872956835 1101 60-61 -0.6884015903885725 1101 62-63 -0.7412853754826614 1101 64-65 -0.7355234807101283 1101 66-67 -0.7813381660801042 1101 68-69 -0.7943020061085733 1101 70-71 -0.8665743071221783 1101 72-73 -0.8811773758710117 1101 74-75 -0.9280106196923654 1101 76-77 -0.8449865193778976 1101 78-79 -0.655256103702861 1101 80-81 -0.5867873517296225 1101 82-83 -0.6775309458550502 1101 84-85 -0.7592753483397061 1101 86-87 -0.8119807549435158 1101 88-89 -0.7753323369265033 1101 90-91 -0.9200886606185037 1101 92-93 -0.9665993785969569 1101 94-95 -1.047273689607561 1101 96-97 -1.0054367233420543 1101 98-99 -1.0904266015523234 1101 100 -1.047210447812244 1102 1 -0.35067567718315473 1102 2 -0.29397513186384927 1102 3 -0.27022110892354334 1102 4 -0.13256178014961506 1102 5 -0.2054602032869397 1102 6 -0.250177066922447 1102 7 -0.28592110582653163 1102 8 -0.30467627075947235 1102 9 -0.4144509758279753 1102 10-11 -0.4011072556525832 1102 12-13 -0.3963334103071219 1102 14-15 -0.517050651734138 1102 16-17 -0.612547001365435 1102 18-19 -0.6188767726539552 1102 20-21 -0.6258422526471392 1102 22-23 -0.6432087075156332 1102 24-25 -0.6764241503865875 1102 26-27 -0.779293688057237 1102 28-29 -0.7255565723835744 1102 30-31 -0.6643821323428156 1102 32-33 -0.6192698417005715 1102 34-35 -0.7023911079683032 1102 36-37 -0.6731465272983641 1102 38-39 -0.7547022734327058 1102 40-41 -0.7860500618329738 1102 42-43 -0.7604471366410479 1102 44-45 -0.784420443960208 1102 46-47 -0.8270974338615886 1102 48-49 -0.9050118732198413 1102 50-51 -0.9886637283267063 1102 52-53 -0.9385439425520445 1102 54-55 -1.045331888814296 1102 56-57 -0.9942385873095674 1102 58-59 -1.0204725662056973 1102 60-61 -1.1289010735890415 1102 62-63 -1.1598796518283052 1102 64-65 -1.085843321604301 1102 66-67 -1.1230526505271996 1102 68-69 -1.0982009611983727 1102 70-71 -1.055048948006693 1102 72-73 -1.1480248148741552 1102 74-75 -1.2358010221519713 1102 76-77 -0.9739965167494624 1102 78-79 -0.8880713778204239 1102 80-81 -0.7583901994186988 1102 82-83 -0.736187766171053 1102 84-85 -0.850490831796094 1102 86-87 -0.9488644675583267 1102 88-89 -0.970960530327325 1102 90-91 -0.9677960050468002 1102 92-93 -1.0369556425748279 1102 94-95 -1.0634091381043937 1102 96-97 -1.2535522385092612 1102 98-99 -1.1807330026366571 1102 100 -1.1773237355862882 1103 1 -0.3433593843555407 1103 2 -0.3071041136446375 1103 3 -0.3670486613738717 1103 4 -0.21322964925973054 1103 5 -0.2069512200591106 1103 6 -0.3084540346622475 1103 7 -0.3598813493530386 1103 8 -0.37113601222863224 1103 9 -0.47398911097489815 1103 10-11 -0.5432423363758616 1103 12-13 -0.639844497515071 1103 14-15 -0.797915011217718 1103 16-17 -0.7954210470839485 1103 18-19 -0.7770645384217403 1103 20-21 -0.7512644991994932 1103 22-23 -0.8408980104000534 1103 24-25 -0.8052396672843471 1103 26-27 -0.9534121359235783 1103 28-29 -0.9078917585468176 1103 30-31 -0.8573081998891965 1103 32-33 -0.7744783323066287 1103 34-35 -0.7736032811102689 1103 36-37 -0.8015964316776092 1103 38-39 -0.8361118383368407 1103 40-41 -0.8733774592114756 1103 42-43 -0.8618534298497593 1103 44-45 -0.9227634144060133 1103 46-47 -0.9709987513602414 1103 48-49 -0.9246607995970919 1103 50-51 -0.7974615468709132 1103 52-53 -0.9785218238110929 1103 54-55 -1.0127971146456218 1103 56-57 -1.0818452392221616 1103 58-59 -1.0790608665670618 1103 60-61 -1.026250805306212 1103 62-63 -1.061537897924353 1103 64-65 -1.074842717359978 1103 66-67 -1.1448682071837126 1103 68-69 -1.061210630248894 1103 70-71 -1.0453720820670682 1103 72-73 -1.0277146280910685 1103 74-75 -1.0415591268349402 1103 76-77 -0.984950775410038 1103 78-79 -0.8468229522386395 1103 80-81 -0.7541574216659015 1103 82-83 -0.7420463489039228 1103 84-85 -0.8054129230229705 1103 86-87 -0.8462261364140495 1103 88-89 -0.856233848948861 1103 90-91 -0.9840142564592327 1103 92-93 -0.944254685999443 1103 94-95 -1.0356561787244623 1103 96-97 -1.1882844116055686 1103 98-99 -1.226462832946666 1103 100 -1.1765638645872798 1104 1 -0.37924203912892196 1104 2 -0.2810302758440315 1104 3 -0.3357167144795312 1104 4 -0.19047528285291548 1104 5 -0.2573981922593944 1104 6 -0.3444832848984305 1104 7 -0.2899724970576756 1104 8 -0.3221628972116619 1104 9 -0.5112353853489253 1104 10-11 -0.43484739680467754 1104 12-13 -0.5136476892162349 1104 14-15 -0.6521059904937871 1104 16-17 -0.6775993392670259 1104 18-19 -0.7004954707627533 1104 20-21 -0.6967800257602264 1104 22-23 -0.7216213135162235 1104 24-25 -0.7342052828376566 1104 26-27 -0.7812582473211904 1104 28-29 -0.8815804612131188 1104 30-31 -0.8141911233494596 1104 32-33 -0.6843980392823283 1104 34-35 -0.7455227303033922 1104 36-37 -0.6988745466106536 1104 38-39 -0.7791292844833819 1104 40-41 -0.8889548045826601 1104 42-43 -0.7401847397613892 1104 44-45 -0.7849151307246203 1104 46-47 -0.7835522544159588 1104 48-49 -0.8736855507676822 1104 50-51 -0.7969648651477357 1104 52-53 -0.7247559652511484 1104 54-55 -0.7195263364693645 1104 56-57 -0.807153628783098 1104 58-59 -0.8239536717744613 1104 60-61 -0.8428217315971764 1104 62-63 -0.8411614379579007 1104 64-65 -0.8129387430853114 1104 66-67 -0.8864219683895698 1104 68-69 -0.8589434882282134 1104 70-71 -0.8003162943152446 1104 72-73 -0.8669172933472851 1104 74-75 -0.8436275012131631 1104 76-77 -0.7420655102205025 1104 78-79 -0.7201282676443199 1104 80-81 -0.8274305724465307 1104 82-83 -0.7849981150147549 1104 84-85 -0.7342043607452631 1104 86-87 -0.7135191234659892 1104 88-89 -0.8299070068601679 1104 90-91 -0.710927630486438 1104 92-93 -0.8271752823495397 1104 94-95 -0.8535175340898746 1104 96-97 -1.0015743684870202 1104 98-99 -0.9474339295652676 1104 100 -0.9921725935170151 1105 1 -0.36666622471429733 1105 2 -0.3452655833088194 1105 3 -0.3834737530165597 1105 4 -0.27480331664695967 1105 5 -0.2568618879446447 1105 6 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68-69 -0.8553585083880151 1105 70-71 -0.8959658968185877 1105 72-73 -0.9052325931916059 1105 74-75 -0.9581451510925163 1105 76-77 -0.8875734420480441 1105 78-79 -0.8244256768833118 1105 80-81 -0.7354959453827234 1105 82-83 -0.7822938943809596 1105 84-85 -0.6865581417843316 1105 86-87 -0.6355461575559609 1105 88-89 -0.8090198134290354 1105 90-91 -0.7779318665145638 1105 92-93 -0.8656726206283274 1105 94-95 -0.8486288522425731 1105 96-97 -0.9452741440294368 1105 98-99 -0.9715665088438534 1105 100 -0.8564769881799101 1106 1 -0.3574088749363611 1106 2 -0.3685226195890472 1106 3 -0.41218777972230214 1106 4 -0.18620339938101438 1106 5 -0.29784682369886184 1106 6 -0.35620414443302906 1106 7 -0.39840999391987 1106 8 -0.4134700538598537 1106 9 -0.5746977606725636 1106 10-11 -0.5159573260932078 1106 12-13 -0.6108396000771421 1106 14-15 -0.7863998883961827 1106 16-17 -0.760650365171422 1106 18-19 -0.7771646694030778 1106 20-21 -0.7305152130243968 1106 22-23 -0.7272691209133626 1106 24-25 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0.689329327577326 2311 30-31 0.7403785877933231 2311 32-33 0.9047495481163566 2311 34-35 0.8862600400320773 2311 36-37 0.9490734564906802 2311 38-39 0.9451276723925659 2311 40-41 0.8949445579063422 2311 42-43 1.023194321483622 2311 44-45 0.9783296210327492 2311 46-47 1.019775927378241 2311 48-49 0.9579489619174737 2311 50-51 0.9743407816696745 2311 52-53 0.9993861690295063 2311 54-55 1.0303173475325593 2311 56-57 1.09076838625748 2311 58-59 1.1162607473917703 2311 60-61 1.1178704266491906 2311 62-63 1.1522820727917633 2311 64-65 1.1371147016135978 2311 66-67 1.1751370857765977 2311 68-69 1.2059432449160568 2311 70-71 1.2043514615352464 2311 72-73 1.1962661558253487 2311 74-75 1.064864992794746 2311 76-77 0.9741093314273179 2311 78-79 0.9340913472734229 2311 80-81 0.9534220678618084 2311 82-83 0.9226322642563183 2311 84-85 0.9365504831623497 2311 86-87 1.0340052936267305 2311 88-89 1.004971489181905 2311 90-91 1.0921297948103188 2311 92-93 1.147667399896541 2311 94-95 1.1587530114906137 2311 96-97 1.2274119336729754 2311 98-99 1.2647953673753456 2311 100 1.362100726367867 2312 1 0.21372977244930524 2312 2 0.23104120245707804 2312 3 0.36442769807552367 2312 4 0.276119994815339 2312 5 0.35343794540442985 2312 6 0.4454947203265718 2312 7 0.4720089808726655 2312 8 0.3360262594479906 2312 9 0.42442315357661897 2312 10-11 0.26805782446600546 2312 12-13 0.48552964958741995 2312 14-15 0.8348918629192994 2312 16-17 0.845726371443547 2312 18-19 0.8898837055501261 2312 20-21 0.8962287627145216 2312 22-23 0.5410455160747034 2312 24-25 0.5177532634367523 2312 26-27 0.815854777330479 2312 28-29 0.9299181642297327 2312 30-31 0.8848289656369488 2312 32-33 0.7510437545642645 2312 34-35 0.9077327183237074 2312 36-37 0.9905838418557948 2312 38-39 1.0102508777471897 2312 40-41 1.0412414527042486 2312 42-43 0.8852625544400894 2312 44-45 0.8199773944140247 2312 46-47 0.9651656140563247 2312 48-49 1.046562060883609 2312 50-51 1.0556882487720642 2312 52-53 1.0141912649633582 2312 54-55 1.0588386308813327 2312 56-57 1.0330767429300636 2312 58-59 1.1036707144522424 2312 60-61 1.117195047209485 2312 62-63 1.1256315481660266 2312 64-65 1.1220721851989168 2312 66-67 1.1013427984818946 2312 68-69 1.1264361525384103 2312 70-71 0.9757552748832481 2312 72-73 1.044661246046033 2312 74-75 1.1506446346587609 2312 76-77 1.0705236180536453 2312 78-79 0.9599269591612654 2312 80-81 0.8640544356644639 2312 82-83 0.8674759018217308 2312 84-85 0.7199510317885824 2312 86-87 0.866931706298228 2312 88-89 0.5210916151939529 2312 90-91 0.9214743103056122 2312 92-93 1.1217606534662785 2312 94-95 1.1742343742568586 2312 96-97 1.254026418705589 2312 98-99 1.3140764500788222 2312 100 1.513952016431542 2313 1 -0.3171447056383414 2313 2 0.01823176096880985 2313 3 0.2603429374795354 2313 4 0.1044206087527968 2313 5 0.24304224258129636 2313 6 0.3526379637517323 2313 7 0.11472580507644636 2313 8 0.03225868938353216 2313 9 0.256094103572714 2313 10-11 0.339023525781073 2313 12-13 0.5563900499788446 2313 14-15 0.6264450958599639 2313 16-17 0.78804819996855 2313 18-19 0.646160679478406 2313 20-21 0.8430538114117851 2313 22-23 0.8607831080355766 2313 24-25 0.7674156678406021 2313 26-27 0.9350663495904783 2313 28-29 0.8247865923473796 2313 30-31 0.8140452769202327 2313 32-33 0.9463880712431632 2313 34-35 0.9972409922337917 2313 36-37 0.8168842841774833 2313 38-39 0.6056308503015089 2313 40-41 0.8038552444983438 2313 42-43 0.8034107318830905 2313 44-45 1.0121367194322133 2313 46-47 1.0910352619177033 2313 48-49 1.1261321306038 2313 50-51 1.05498178683429 2313 52-53 0.9951206703763447 2313 54-55 1.0966693284480868 2313 56-57 1.1658289574287153 2313 58-59 1.0152166299581893 2313 60-61 0.9968257187512748 2313 62-63 1.0872331761780387 2313 64-65 1.1401855176382014 2313 66-67 1.189069402601291 2313 68-69 1.1911872528190557 2313 70-71 1.067717688137627 2313 72-73 1.1532043559037675 2313 74-75 0.7613862023637523 2313 76-77 0.9889247232412721 2313 78-79 0.8314076314480943 2313 80-81 0.9507102154552172 2313 82-83 0.9555029539790532 2313 84-85 0.9874698908981969 2313 86-87 1.0836817996207309 2313 88-89 1.050476208849929 2313 90-91 1.1965395991262255 2313 92-93 0.913985047326026 2313 94-95 0.9555332192415698 2313 96-97 1.2470067424942108 2313 98-99 1.2605956154891977 2313 100 1.4108857106975208 2314 1 -0.31064710039635557 2314 2 -0.0843517906248934 2314 3 0.13325582270130099 2314 4 -0.1487153729074322 2314 5 0.17376133632294 2314 6 0.23086493675006636 2314 7 0.16649082496330436 2314 8 0.15441034239233176 2314 9 0.27444400293362037 2314 10-11 0.4336144925925467 2314 12-13 0.5247011754425799 2314 14-15 0.5967788845444275 2314 16-17 0.7780762347056438 2314 18-19 0.8199794072138786 2314 20-21 0.45016566888784126 2314 22-23 0.6802248281526744 2314 24-25 0.8373239548417573 2314 26-27 0.8438005391347332 2314 28-29 0.7920975276836231 2314 30-31 0.7955665973848056 2314 32-33 0.8968910961327055 2314 34-35 0.9178165265086164 2314 36-37 0.8040462070210097 2314 38-39 0.8492998371217979 2314 40-41 0.9266892480213258 2314 42-43 0.8083776741044986 2314 44-45 0.8563126391132201 2314 46-47 0.9228155826719302 2314 48-49 0.8314276912469083 2314 50-51 0.9689335404944686 2314 52-53 1.0313431679716274 2314 54-55 1.0976975259583952 2314 56-57 0.981219019668572 2314 58-59 0.9982816605039915 2314 60-61 1.0378316621868393 2314 62-63 0.7575576352152495 2314 64-65 0.8936446637721644 2314 66-67 0.9698347865149728 2314 68-69 1.055420008508257 2314 70-71 1.134517109542756 2314 72-73 1.1199544981459226 2314 74-75 1.1369167520946633 2314 76-77 0.8641598085300224 2314 78-79 0.8447740146713372 2314 80-81 0.8691260155347891 2314 82-83 0.8401698500731847 2314 84-85 0.7513422050170746 2314 86-87 0.8987391387629167 2314 88-89 0.9814218665668548 2314 90-91 1.0809779180943764 2314 92-93 1.0731502970009714 2314 94-95 1.1652768133038371 2314 96-97 1.2704341613731494 2314 98-99 1.2672841703801687 2314 100 1.223903397778539 2315 1 -0.05870579708704682 2315 2 0.10520980343960673 2315 3 0.12121030749604245 2315 4 -0.03179374101230792 2315 5 0.24665289519683853 2315 6 0.35361700609556124 2315 7 0.4322747671130145 2315 8 0.39418296884011284 2315 9 0.6369470309787602 2315 10-11 0.6070819965308161 2315 12-13 0.6237369047433248 2315 14-15 0.7629344603057504 2315 16-17 0.7285899764096442 2315 18-19 0.7895509533325153 2315 20-21 0.8081284348722946 2315 22-23 0.8664974121715687 2315 24-25 0.8314526750460303 2315 26-27 0.9425467985017306 2315 28-29 0.9593285354339685 2315 30-31 0.935348821708196 2315 32-33 0.9486315859555816 2315 34-35 0.9691428737408998 2315 36-37 0.9668994608217218 2315 38-39 0.9900237547739508 2315 40-41 0.9879677778274427 2315 42-43 1.054396965774032 2315 44-45 0.9806856040976513 2315 46-47 0.9894462275887292 2315 48-49 1.0704293825400626 2315 50-51 1.0238847931985546 2315 52-53 1.0468447570002652 2315 54-55 0.9717412439191335 2315 56-57 1.0725488878343796 2315 58-59 1.1063957758602996 2315 60-61 1.1903765376948954 2315 62-63 1.157877479282405 2315 64-65 1.1658578528972185 2315 66-67 1.2149961698371996 2315 68-69 1.1069486938265527 2315 70-71 0.7631995614711755 2315 72-73 1.0261465814941673 2315 74-75 1.1348685379401857 2315 76-77 1.1155219906517608 2315 78-79 1.0052233572289495 2315 80-81 1.0121962073133943 2315 82-83 0.9997356803788726 2315 84-85 0.9250249434312039 2315 86-87 0.9376896736208487 2315 88-89 1.0709443438486517 2315 90-91 1.1147178959298074 2315 92-93 1.15201062542722 2315 94-95 1.264284687309111 2315 96-97 1.3008134019660211 2315 98-99 1.1859401649908499 2315 100 1.0859532616434322 2316 1 0.5158622709359264 2316 2 0.49623988616695414 2316 3 0.47638746383781694 2316 4 0.2948607298443804 2316 5 0.42152910554396783 2316 6 0.4486440653903969 2316 7 0.45676265268285476 2316 8 0.4598034330825129 2316 9 0.48012351639371076 2316 10-11 0.45931460905413957 2316 12-13 0.6593607829804569 2316 14-15 0.8075693896575231 2316 16-17 0.8112997064924912 2316 18-19 0.8310700759647958 2316 20-21 0.8956685793711827 2316 22-23 0.9134996112529663 2316 24-25 0.9188774697497308 2316 26-27 1.0013908104493439 2316 28-29 0.9365961022100606 2316 30-31 0.8178616649037025 2316 32-33 0.786546524062274 2316 34-35 0.7312673647619263 2316 36-37 0.7455878841071382 2316 38-39 0.6680294323857012 2316 40-41 0.79173521918527 2316 42-43 0.9125983993778419 2316 44-45 0.8255541401864548 2316 46-47 0.871189278138381 2316 48-49 0.6282145918911581 2316 50-51 0.6651551036513794 2316 52-53 0.8495772845922858 2316 54-55 0.9385901526584703 2316 56-57 0.9540568552426834 2316 58-59 0.8851708351600038 2316 60-61 0.9848340854390685 2316 62-63 1.0338234815448075 2316 64-65 0.9893395917544723 2316 66-67 0.9807067860670529 2316 68-69 0.9098025681550155 2316 70-71 0.7662286180214224 2316 72-73 0.8219288292086375 2316 74-75 0.8928230162854405 2316 76-77 0.7340580642321051 2316 78-79 0.7297801924025578 2316 80-81 0.6516901293982116 2316 82-83 0.6271597231818156 2316 84-85 0.6836096942141552 2316 86-87 0.6953053856678331 2316 88-89 0.6262333872847776 2316 90-91 0.7763279133178926 2316 92-93 0.7528618866722461 2316 94-95 0.7000059854003204 2316 96-97 0.8732819317230245 2316 98-99 0.9349294368586811 2316 100 0.5768223097553395 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 2.0 11 12.0 12 45.0 13 233.0 14 942.0 15 2825.0 16 6562.0 17 13129.0 18 21698.0 19 30799.0 20 42021.0 21 55726.0 22 67498.0 23 72569.0 24 75586.0 25 83407.0 26 98209.0 27 119834.0 28 147173.0 29 180126.0 30 219728.0 31 272994.0 32 346087.0 33 449824.0 34 630498.0 35 858748.0 36 1303390.0 37 2014796.0 38 1818614.0 39 279558.0 40 36.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.71640342271974 19.75061031175177 13.592203908797124 23.94078235673136 2 15.4880523765697 20.92408833965488 40.55444735939172 23.033411924383692 3 17.811309621565695 27.440397565569764 30.745921730173958 24.002371082690583 4 12.331565716742382 17.57570362232028 38.479614507954274 31.61311615298306 5 12.672039828364792 37.92900796313288 34.426408349072204 14.972543859430118 6 29.273756527425064 37.48705586698663 19.37884877648907 13.860338829099236 7 26.480702842134924 33.03138987557546 23.206314533534968 17.281592748754647 8 25.973081760077843 35.317571362296015 20.796659979476022 17.912686898150127 9 27.42690437446479 15.622860580427414 20.508121205489896 36.4421138396179 10-11 24.812415580837758 25.998656945978954 29.02951619824858 20.15941127493471 12-13 25.261018958181936 24.02646579782462 28.58128691597256 22.13122832802089 14-15 22.913244973088677 25.437226904484206 26.498472483260816 25.151055639166298 16-17 22.21994878136756 27.790010336859165 27.195155190198534 22.794885691574738 18-19 21.795236537858898 27.27643856519756 29.035499918644643 21.892824978298904 20-21 22.95266691473089 26.74172129072707 28.420491219264605 21.885120575277433 22-23 22.658238345478384 26.612624419698573 28.374453700659387 22.354683534163662 24-25 22.197861433030585 26.397694174144732 28.80772441023984 22.596719982584844 26-27 22.19310651302169 26.621970597656464 28.489079221033386 22.695843668288457 28-29 22.150727557131013 27.359227612300163 28.403913758970006 22.086131071598817 30-31 23.061923112576643 26.66206860918313 28.519180173731897 21.756828104508333 32-33 22.944648415764448 26.725512960925528 28.193080562088372 22.13675806122165 34-35 22.847200398715803 26.920048003541147 28.458161196421006 21.774590401322047 36-37 22.516069194552184 26.925284841525244 28.399818976579226 22.158826987343343 38-39 22.544330844696535 26.873168114948555 28.45764520537252 22.124855834982394 40-41 22.585424174791672 26.291059198601573 28.848958202455893 22.274558424150857 42-43 22.378593579359016 26.626856294383277 28.259457496205975 22.735092630051735 44-45 21.80685373473673 27.04380005580897 28.67849489032006 22.470851319134233 46-47 21.83404895184532 26.458340276738152 28.329205648795906 23.37840512262062 48-49 22.108246057385024 26.542168177712938 28.3525742062665 22.997011558635545 50-51 21.986848053066335 26.83557547801083 28.197064477454582 22.980511991468255 52-53 21.75854392226256 27.0333875191119 27.992702154325737 23.215366404299804 54-55 21.63291224574271 26.722302668723884 28.37667330562895 23.26811177990445 56-57 22.01995585399198 26.670771286196317 27.92127271717762 23.38800014263408 58-59 21.52279927812965 26.84669351996577 28.30845980963652 23.322047392268058 60-61 22.044101891662766 27.07050614760549 27.823833409497716 23.061558551234025 62-63 22.070616633819352 26.85554401758162 27.66028649212009 23.413552856478937 64-65 21.607059716204976 26.578740848419024 27.728969406220894 24.085230029155106 66-67 21.628445203933833 26.468872434089885 27.633325898194784 24.2693564637815 68-69 21.328016886820464 26.66132980235791 27.82867384026376 24.181979470557863 70-71 21.59809353765497 26.421540357328116 27.83193718639228 24.14842891862463 72-73 21.644234812130144 26.850085047901338 27.34636974032157 24.15931039964695 74-75 21.80466410211824 26.87305202996047 27.19063381558997 24.131650052331317 76-77 21.678571292849462 26.721000694353584 27.239149164414133 24.36127884838282 78-79 21.594903470359895 26.698223518521313 27.400631795632492 24.3062412154863 80-81 21.57947985607985 26.918249227310874 27.14571809437198 24.356552822237294 82-83 21.961447140467843 26.709974519299884 27.31836043616427 24.01021790406801 84-85 22.149870084343636 26.9166930378544 26.761213438076727 24.172223439725236 86-87 21.5673750581747 27.070377228346885 26.98260273077783 24.379644982700576 88-89 21.685490965254438 26.58114088809475 27.09026393407467 24.64310421257614 90-91 22.130608360704564 26.352928235850705 27.10832861808698 24.408134785357753 92-93 22.019975899673067 26.57361525044628 26.93762280138261 24.46878604849804 94-95 21.59503514995723 26.35115653997629 27.13590267434943 24.917905635717048 96-97 21.526845679474018 26.302560077305014 27.259032695171122 24.91156154804985 98-99 21.506060977501786 26.484855617571977 27.04044115052554 24.9686422544007 100 21.511717916242624 26.442442669791067 27.08198665543075 24.96385275853556 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1572.0 1 1124.0 2 1472.5 3 2288.0 4 1732.5 5 1239.5 6 1692.5 7 3087.5 8 4894.5 9 5771.0 10 5453.0 11 5175.5 12 5612.5 13 6323.0 14 7195.0 15 8210.5 16 9180.5 17 10243.5 18 11447.5 19 12524.5 20 13717.5 21 15260.5 22 17636.5 23 21110.0 24 25548.5 25 31377.5 26 39110.0 27 48810.5 28 60552.0 29 75802.5 30 93677.0 31 110791.0 32 132129.5 33 153625.5 34 172439.0 35 192286.0 36 214143.5 37 241991.0 38 258319.5 39 260154.0 40 263400.0 41 268014.5 42 275633.0 43 288930.0 44 305375.5 45 327983.0 46 350470.5 47 369718.5 48 387654.5 49 400236.0 50 407845.5 51 407962.0 52 397170.5 53 375288.5 54 349046.0 55 316588.5 56 280539.5 57 241544.5 58 204092.5 59 172958.5 60 137296.0 61 104328.5 62 80454.5 63 61488.0 64 44407.0 65 30462.5 66 21589.5 67 16052.0 68 11932.5 69 8610.5 70 6370.0 71 4621.5 72 3338.0 73 2406.0 74 1749.5 75 1191.5 76 758.5 77 448.5 78 268.5 79 166.5 80 103.5 81 67.5 82 47.0 83 33.5 84 28.0 85 22.0 86 12.5 87 8.5 88 6.5 89 3.0 90 2.0 91 2.5 92 2.0 93 1.5 94 1.5 95 0.5 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05069106466323712 2 0.0 3 0.0 4 0.01719371443823717 5 0.003690570018308484 6 1.0854617700907305E-5 7 1.8452850091542418E-4 8 3.364931487281265E-4 9 0.001975540421565129 10-11 9.063605780257599E-4 12-13 2.605108248217753E-4 14-15 4.341847080362922E-5 16-17 6.512770620544383E-5 18-19 0.0 20-21 1.0854617700907305E-5 22-23 0.0 24-25 4.884577965408287E-5 26-27 2.170923540181461E-5 28-29 0.001014906755034833 30-31 9.009332691753063E-4 32-33 0.01379079178900273 34-35 0.022778415245353976 36-37 0.030512330357250435 38-39 0.02029813510069666 40-41 1.1397348585952671E-4 42-43 0.0017584480675469835 44-45 0.00964432782725614 46-47 0.014002456834170422 48-49 0.005525000409761818 50-51 0.010344450668964661 52-53 0.015820605299072397 54-55 0.009638900518405687 56-57 0.0030881387359081285 58-59 0.008309209850044541 60-61 0.0015087918604261153 62-63 0.0010257613727357403 64-65 0.01910955446244731 66-67 0.04497068113485896 68-69 0.05443590777005013 70-71 0.04786343675215076 72-73 0.04060712481909423 74-75 0.028227433331209445 76-77 0.028916701555217063 78-79 0.012439391885239771 80-81 0.002165496231331007 82-83 0.027798675932023606 84-85 0.047619207853880345 86-87 0.04790685522295439 88-89 0.059315058426607975 90-91 0.034539393524287046 92-93 0.02627360214504613 94-95 0.00769592394994328 96-97 0.0035820238412994105 98-99 0.0219914554620382 100 0.018789343240270547 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 9212669.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.903340350701875 #Duplication Level Percentage of deduplicated Percentage of total 1 76.09811948295942 46.34629670919054 2 14.480064321720512 17.637685713715985 3 4.576202850273655 8.361181191122052 4 1.808800379316957 4.406479405120772 5 0.8919641240437162 2.716179731362506 6 0.5188997221203431 1.8961635830507928 7 0.335475074697181 1.4302086857421685 8 0.23413932108213883 1.1407893409078214 9 0.16505245527371382 0.9047021273328592 >10 0.8287821092791291 8.86000691723042 >50 0.04202537636903198 1.7501531650089626 >100 0.01830370742071641 2.094556718164456 >500 0.0012758711968226982 0.5173231925256608 >1k 8.056838236894141E-4 0.978309911337903 >5k 2.2380105758524316E-5 0.10725629545818285 >10k+ 6.714031727557294E-5 0.8527073127289261 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 27118 0.29435552281320426 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21238 0.23053037073186936 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14204 0.15417898982368736 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 3.2563853102721915E-5 7 0.0 0.0 0.0 1.0854617700907305E-5 3.2563853102721915E-5 8 0.0 0.0 0.0 1.0854617700907305E-5 5.4273088504536525E-5 9 0.0 0.0 0.0 6.512770620544383E-5 8.683694160725844E-5 10-11 0.0 0.0 0.0 9.22642504577121E-5 9.22642504577121E-5 12-13 0.0 1.0854617700907305E-5 0.0 1.3025541241088766E-4 1.0854617700907305E-4 14-15 0.0 1.0854617700907305E-5 0.0 1.5196464781270228E-4 1.3025541241088766E-4 16-17 0.0 1.0854617700907305E-5 0.0 2.1166504516769244E-4 1.5739195666315592E-4 18-19 0.0 1.0854617700907305E-5 0.0 2.7136544252268264E-4 2.0081042746678516E-4 20-21 0.0 1.0854617700907305E-5 0.0 4.2875739918583856E-4 2.062377363172388E-4 22-23 0.0 1.0854617700907305E-5 0.0 0.0011831533293988963 2.279469717190534E-4 24-25 0.0 1.0854617700907305E-5 0.0 0.0035223234439444202 2.49656207120868E-4 26-27 0.0 1.0854617700907305E-5 0.0 0.007261739241906987 2.49656207120868E-4 28-29 0.0 1.0854617700907305E-5 0.0 0.01845285009154242 2.49656207120868E-4 30-31 0.0 1.0854617700907305E-5 0.0 0.05772485693342505 2.49656207120868E-4 32-33 0.0 1.0854617700907305E-5 0.0 0.15130251613294693 2.49656207120868E-4 34-35 0.0 1.0854617700907305E-5 0.0 0.27801932317333883 2.49656207120868E-4 36-37 0.0 1.0854617700907305E-5 0.0 0.4701894749502017 2.49656207120868E-4 38-39 0.0 2.170923540181461E-5 0.0 0.7899936489631831 2.49656207120868E-4 40-41 0.0 2.170923540181461E-5 0.0 1.1787626365388792 2.49656207120868E-4 42-43 0.0 2.170923540181461E-5 0.0 1.550229363499329 2.49656207120868E-4 44-45 0.0 2.170923540181461E-5 0.0 1.9632150031657494 2.605108248217753E-4 46-47 0.0 2.170923540181461E-5 0.0 2.4279337507946934 2.767927513731363E-4 48-49 0.0 2.170923540181461E-5 0.0 2.929053458883631 2.9307467792449724E-4 50-51 0.0 3.2563853102721915E-5 0.0 3.4645768777755936 2.9307467792449724E-4 52-53 0.0 3.2563853102721915E-5 0.0 4.00505000233917 2.985019867749509E-4 54-55 0.0 3.2563853102721915E-5 0.0 4.568757436091539 3.0392929562540456E-4 56-57 0.0 3.2563853102721915E-5 0.0 5.161506399502684 3.0392929562540456E-4 58-59 0.0 3.2563853102721915E-5 0.0 5.831931007181524 3.093566044758582E-4 60-61 0.0 3.2563853102721915E-5 0.0 6.514626760171238 3.2563853102721916E-4 62-63 0.0 3.2563853102721915E-5 0.0 7.227623178473035 3.799116195317557E-4 64-65 0.0 3.2563853102721915E-5 0.0 7.880680397830423 3.799116195317557E-4 66-67 0.0 3.2563853102721915E-5 0.0 8.588922493579222 3.799116195317557E-4 68-69 0.0 3.2563853102721915E-5 0.0 9.358976209825839 3.799116195317557E-4 70-71 0.0 3.2563853102721915E-5 0.0 10.145963129685871 3.799116195317557E-4 72-73 0.0 3.2563853102721915E-5 0.0 10.892608862860481 4.2333009033538487E-4 74-75 0.0 3.2563853102721915E-5 0.0 11.625404103848734 4.2333009033538487E-4 76-77 0.0 3.2563853102721915E-5 0.0 12.399821376411113 4.2333009033538487E-4 78-79 0.0 3.2563853102721915E-5 0.0 13.211871608542541 4.2333009033538487E-4 80-81 0.0 3.2563853102721915E-5 0.0 14.024627390824527 4.450393257371995E-4 82-83 5.4273088504536525E-6 3.2563853102721915E-5 0.0 14.84917671523855 4.667485611390141E-4 84-85 1.0854617700907305E-5 3.2563853102721915E-5 0.0 15.641308723888809 4.7217586998946775E-4 86-87 1.0854617700907305E-5 3.2563853102721915E-5 0.0 16.417571281460347 4.830304876903751E-4 88 1.0854617700907305E-5 3.2563853102721915E-5 0.0 17.011411133950432 4.884577965408287E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 13865 0.0 51.949127 1 TACCTGG 9820 0.0 48.238113 2 ACCTGGG 11030 0.0 42.43601 3 GTATCAA 25315 0.0 41.842983 1 ATCAACG 31760 0.0 32.70111 3 CAACGCA 32020 0.0 32.48776 5 TATCAAC 32965 0.0 31.90423 2 CCTGGGG 10345 0.0 31.481699 4 TCAACGC 33310 0.0 31.405518 4 GTACCTG 15355 0.0 31.261894 1 AACGCAG 34120 0.0 31.02393 6 GTACATG 32585 0.0 27.688318 1 ACGCAGA 38360 0.0 27.27635 7 TACATGG 32520 0.0 27.196333 2 TGGGGGG 18900 0.0 26.698513 6 CGCAGAG 39165 0.0 26.559755 8 ACATGGG 32420 0.0 26.19364 3 CTGGGGG 7485 0.0 25.79963 5 ATGGGGG 18185 0.0 25.243217 5 CATGGGG 24925 0.0 24.661983 4 >>END_MODULE