FastQCFastQC Report
Mon 6 Jun 2016
ERR1211426_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211426_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19758039
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT566630.28678453362704664No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT402510.20371961002810046No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT252220.12765436893813195No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA313250.051.306021
GTATCAA636050.046.0688061
CAACGCA752550.037.7231145
ATCAACG760500.037.365953
TATCAAC801100.036.363922
AACGCAG830300.034.7686166
TCAACGC857700.033.3833544
ACGCAGA890750.031.5860257
CGCAGAG898000.031.2346528
GCAGAGT971150.027.9376839
GTACTAG126450.026.7164021
GTACATG855700.025.6040741
GTGGTAT107400.025.5765481
TACATGG858200.025.147092
ACATGGG856400.024.366183
TAGGCAT160100.023.4015625
GCTATTG205550.023.3030978
TATTCTC110950.022.964125
GAGTACT488800.022.88002612-13
GCATCGT127350.022.8480268
Produced by FastQC (version 0.11.5)