FastQCFastQC Report
Mon 6 Jun 2016
ERR1211426_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211426_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19758039
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT499670.25289453067685513No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT376770.19069200136713974No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT238310.12061419658094613No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT205980.10425123667384198No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA317150.051.7982441
GTATCAA608950.043.3921851
CAACGCA737800.034.988065
ATCAACG743500.034.6812973
TATCAAC781000.033.65322
AACGCAG812350.032.615036
TCAACGC839050.030.9337754
ACGCAGA886050.028.9901037
CGCAGAG895800.028.559188
GTACTAG124400.026.1164721
GCAGAGT970800.025.7815869
GCTATTG216400.025.2945848
AGGGCTA226000.024.6989155
GTGGTAT109300.024.6485461
ATTTAGG233350.024.4807821
CTATTGA227050.024.2114649
GGGCTAT240250.024.152526
GTACATG850000.024.1502511
TACATGG850950.023.7187422
ACATGGG838000.023.2109413
Produced by FastQC (version 0.11.5)