##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211426_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 19758039 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.11158111389496 33.0 31.0 34.0 30.0 34.0 2 32.260678552157934 34.0 31.0 34.0 30.0 34.0 3 32.33639881974117 34.0 31.0 34.0 30.0 34.0 4 35.96951013205309 37.0 35.0 37.0 35.0 37.0 5 35.83168010752484 37.0 35.0 37.0 35.0 37.0 6 35.86307664439776 37.0 35.0 37.0 35.0 37.0 7 35.79387822850233 37.0 35.0 37.0 33.0 37.0 8 35.78301591569892 37.0 35.0 37.0 33.0 37.0 9 37.36738969894735 39.0 37.0 39.0 34.0 39.0 10-11 37.351495636788655 39.0 37.0 39.0 34.0 39.0 12-13 37.26745926050657 39.0 37.0 39.0 33.5 39.0 14-15 38.48256360360459 40.0 38.0 41.0 34.0 41.0 16-17 38.48654942426219 40.0 38.0 41.0 34.0 41.0 18-19 38.495758258195565 40.0 38.0 41.0 34.0 41.0 20-21 38.45875223244574 40.0 38.0 41.0 34.0 41.0 22-23 38.360738583419135 40.0 38.0 41.0 34.0 41.0 24-25 38.2395823289953 40.0 38.0 41.0 33.5 41.0 26-27 38.108968658276254 40.0 37.5 41.0 33.0 41.0 28-29 38.061958223688094 40.0 37.0 41.0 33.0 41.0 30-31 38.11013605651857 40.0 37.0 41.0 33.5 41.0 32-33 37.96966366449626 40.0 37.0 41.0 33.0 41.0 34-35 37.87069012769942 40.0 37.0 41.0 33.0 41.0 36-37 37.7407324684398 40.0 37.0 41.0 33.0 41.0 38-39 37.626019945602906 40.0 37.0 41.0 33.0 41.0 40-41 37.56011828906705 40.0 37.0 41.0 32.0 41.0 42-43 37.43440945733531 40.0 36.0 41.0 32.0 41.0 44-45 37.266876788733946 40.0 36.0 41.0 31.5 41.0 46-47 37.039838568999684 39.0 36.0 41.0 31.0 41.0 48-49 36.81201940131812 39.0 35.5 41.0 31.0 41.0 50-51 36.62469640838344 39.0 35.0 41.0 30.0 41.0 52-53 36.414615666058765 39.0 35.0 40.0 30.0 41.0 54-55 36.21681845551575 38.5 35.0 40.0 29.5 41.0 56-57 35.97302576434838 38.0 35.0 40.0 29.0 41.0 58-59 35.6948120205654 38.0 34.5 40.0 28.5 41.0 60-61 35.448646902660734 37.5 34.0 40.0 28.0 41.0 62-63 35.15948801902861 37.0 34.0 40.0 28.0 41.0 64-65 34.82472258507031 36.5 34.0 39.5 27.5 41.0 66-67 34.48977130270873 36.0 33.5 39.0 26.5 41.0 68-69 34.15315550799348 35.5 33.0 39.0 26.0 40.0 70-71 33.83441696820216 35.0 33.0 38.5 26.0 40.0 72-73 33.5150155336772 35.0 33.0 38.0 26.0 40.0 74-75 33.17084246063084 35.0 32.0 37.0 25.5 39.5 76-77 32.157731316351786 34.0 31.0 36.0 24.5 39.0 78-79 32.651757317616386 35.0 32.0 36.0 25.0 39.0 80-81 32.55317144580998 35.0 32.0 36.0 25.0 37.5 82-83 32.28488755893234 35.0 32.0 35.5 25.0 37.0 84-85 32.016370070936695 34.5 31.5 35.0 25.0 37.0 86-87 31.766987553774946 34.0 31.0 35.0 24.0 36.0 88-89 31.536547174545003 34.0 31.0 35.0 24.0 36.0 90-91 31.32023413355951 34.0 31.0 35.0 23.5 35.5 92-93 31.137501297573102 34.0 31.0 35.0 23.0 35.0 94-95 30.977671468307154 34.0 31.0 35.0 23.0 35.0 96-97 30.812754241450783 34.0 30.0 35.0 21.0 35.0 98-99 30.63268596645649 34.0 30.0 35.0 20.0 35.0 100 28.808450828546295 32.0 26.0 34.0 17.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.24397023299596654 1101 2 -0.20269626221498527 1101 3 -0.23146356168054893 1101 4 -0.10405688710337557 1101 5 -0.15349368299959565 1101 6 -0.20581309458601993 1101 7 -0.22591223161543894 1101 8 -0.2935738428951211 1101 9 -0.392318986770249 1101 10-11 -0.3500856247029347 1101 12-13 -0.37176592857091606 1101 14-15 -0.5187323168166884 1101 16-17 -0.5303695354353479 1101 18-19 -0.5339159505520428 1101 20-21 -0.5556890396465164 1101 22-23 -0.6091109953442668 1101 24-25 -0.6443549107376327 1101 26-27 -0.6482601278786575 1101 28-29 -0.6576135550764093 1101 30-31 -0.5868225428942182 1101 32-33 -0.509879865080471 1101 34-35 -0.5693848102091437 1101 36-37 -0.6002489169069136 1101 38-39 -0.6181745161911607 1101 40-41 -0.7029481697486091 1101 42-43 -0.6976094467369975 1101 44-45 -0.6618924788635923 1101 46-47 -0.6786632074319314 1101 48-49 -0.6439436655989184 1101 50-51 -0.6969666477722356 1101 52-53 -0.7027734130660122 1101 54-55 -0.7051170861918408 1101 56-57 -0.747722971869166 1101 58-59 -0.6761514284754426 1101 60-61 -0.6856830181045765 1101 62-63 -0.7699489374114918 1101 64-65 -0.7358662179961399 1101 66-67 -0.7294395791484689 1101 68-69 -0.8281264183620252 1101 70-71 -0.820238348397929 1101 72-73 -0.8275723467280827 1101 74-75 -0.8612356644204908 1101 76-77 -0.723860232595932 1101 78-79 -0.6758662865389056 1101 80-81 -0.6060661765257649 1101 82-83 -0.6372610237157836 1101 84-85 -0.7103867096739727 1101 86-87 -0.7676037112330221 1101 88-89 -0.8143529431633887 1101 90-91 -0.8855578684139509 1101 92-93 -0.9868883143284322 1101 94-95 -1.0824577463053338 1101 96-97 -1.102392533288974 1101 98-99 -1.1746527963892 1101 100 -1.090393389099507 1102 1 -0.2849490948898534 1102 2 -0.2402698901077187 1102 3 -0.31580954779827763 1102 4 -0.15968958846431036 1102 5 -0.1813380976239074 1102 6 -0.27453592995006915 1102 7 -0.2710760342212666 1102 8 -0.31902150621077396 1102 9 -0.45639755005610283 1102 10-11 -0.43182563548592867 1102 12-13 -0.4171406118882075 1102 14-15 -0.5676962535336543 1102 16-17 -0.6362925465221636 1102 18-19 -0.644074975814064 1102 20-21 -0.662856535930608 1102 22-23 -0.6421586108847919 1102 24-25 -0.6900114821433192 1102 26-27 -0.786453061895152 1102 28-29 -0.7606732109462158 1102 30-31 -0.6759166353814194 1102 32-33 -0.6546185011612593 1102 34-35 -0.7223248136634552 1102 36-37 -0.6714513271117326 1102 38-39 -0.7488142241808475 1102 40-41 -0.7705988934291383 1102 42-43 -0.7727178287063907 1102 44-45 -0.7635877699399813 1102 46-47 -0.7792822543151061 1102 48-49 -0.7750723013326919 1102 50-51 -0.8558041720609637 1102 52-53 -0.9004341554901174 1102 54-55 -0.918639761424366 1102 56-57 -0.9110998678370805 1102 58-59 -0.9683556538889846 1102 60-61 -0.9597309532964218 1102 62-63 -0.9532088146948539 1102 64-65 -0.8689579338883746 1102 66-67 -0.9271024890693056 1102 68-69 -0.9508636020144579 1102 70-71 -0.9380511911697837 1102 72-73 -1.0400184464303095 1102 74-75 -1.0277928901429831 1102 76-77 -0.8952435684926776 1102 78-79 -0.8211564945473881 1102 80-81 -0.726779424442789 1102 82-83 -0.7465855780768464 1102 84-85 -0.7377842073347374 1102 86-87 -0.7975839814291454 1102 88-89 -0.8812137669160229 1102 90-91 -0.8866524143789007 1102 92-93 -0.8963708713777976 1102 94-95 -0.9334571566511194 1102 96-97 -1.0253275813211467 1102 98-99 -1.1061609229112115 1102 100 -1.0974351027719358 1103 1 -0.31978206305530676 1103 2 -0.29317885519844467 1103 3 -0.32211114433805577 1103 4 -0.20247599077835332 1103 5 -0.18402652337086067 1103 6 -0.26218969087344135 1103 7 -0.2769457448729469 1103 8 -0.30426485639637946 1103 9 -0.4305182603396176 1103 10-11 -0.4092431181807257 1103 12-13 -0.5159025852100854 1103 14-15 -0.692017219974197 1103 16-17 -0.7746550141056545 1103 18-19 -0.7249371971792229 1103 20-21 -0.6706148312998721 1103 22-23 -0.7391508320147437 1103 24-25 -0.6695430579473864 1103 26-27 -0.7709347933278607 1103 28-29 -0.7674469886201578 1103 30-31 -0.6649945856823436 1103 32-33 -0.6939896845605702 1103 34-35 -0.6951433069449067 1103 36-37 -0.6544906212593844 1103 38-39 -0.7197404244999888 1103 40-41 -0.7936765100366685 1103 42-43 -0.7618238552830903 1103 44-45 -0.7062561637489821 1103 46-47 -0.7755277127715701 1103 48-49 -0.7756562662880029 1103 50-51 -0.8427142217099188 1103 52-53 -0.8609672066710203 1103 54-55 -0.87622852709152 1103 56-57 -0.8913350572398997 1103 58-59 -0.9367506458789592 1103 60-61 -0.8893069073671427 1103 62-63 -0.8868812195905846 1103 64-65 -0.8840183011032039 1103 66-67 -0.9326515490161995 1103 68-69 -0.950677863027579 1103 70-71 -0.9572469229825487 1103 72-73 -0.9790751679420922 1103 74-75 -0.9628023832190564 1103 76-77 -0.8991610205215039 1103 78-79 -0.8355066477918136 1103 80-81 -0.7388024474387791 1103 82-83 -0.751563066812551 1103 84-85 -0.7766893798759291 1103 86-87 -0.8220382656502849 1103 88-89 -0.79304949802167 1103 90-91 -0.9026044138380094 1103 92-93 -0.9212162872696936 1103 94-95 -0.8850386084317208 1103 96-97 -1.001211841605322 1103 98-99 -1.090164436673689 1103 100 -1.0972776330719292 1104 1 -0.3399017418358987 1104 2 -0.3234176011464598 1104 3 -0.36991700445630116 1104 4 -0.20174218276237355 1104 5 -0.21136395558949062 1104 6 -0.2990291552682507 1104 7 -0.25665409514643045 1104 8 -0.2963663569942483 1104 9 -0.4689504138643912 1104 10-11 -0.4152924689680404 1104 12-13 -0.516562514054975 1104 14-15 -0.6700362604152659 1104 16-17 -0.673275783509176 1104 18-19 -0.6952479020849083 1104 20-21 -0.6732370368107965 1104 22-23 -0.7197955495149628 1104 24-25 -0.7621329049777046 1104 26-27 -0.7972511796215258 1104 28-29 -0.8102028200772864 1104 30-31 -0.7760217614913287 1104 32-33 -0.7000677311473353 1104 34-35 -0.765336913881633 1104 36-37 -0.7035922207826886 1104 38-39 -0.8177416804519879 1104 40-41 -0.871320610185478 1104 42-43 -0.7252340244147888 1104 44-45 -0.8117400665692642 1104 46-47 -0.8594150729520749 1104 48-49 -0.8721197587230947 1104 50-51 -0.8502539391882067 1104 52-53 -0.8169243162006836 1104 54-55 -0.8000083245903014 1104 56-57 -0.8318248433456148 1104 58-59 -0.8669517883572695 1104 60-61 -0.8780836885742715 1104 62-63 -0.9186515085643876 1104 64-65 -0.8629789936783538 1104 66-67 -0.9634387235215129 1104 68-69 -0.8422441771535034 1104 70-71 -0.8554282388008261 1104 72-73 -0.8628240802841276 1104 74-75 -0.8708302405407196 1104 76-77 -0.8215627548498006 1104 78-79 -0.7264202819454795 1104 80-81 -0.7501235800173625 1104 82-83 -0.8333511428311695 1104 84-85 -0.740227810696819 1104 86-87 -0.646874119075104 1104 88-89 -0.7844356345346633 1104 90-91 -0.7579700969015732 1104 92-93 -0.8829112210102465 1104 94-95 -0.9547604134038643 1104 96-97 -1.0475243883240424 1104 98-99 -0.9819687861299222 1104 100 -1.099752974750011 1105 1 -0.35446652456282735 1105 2 -0.31879410747738035 1105 3 -0.35380258027081624 1105 4 -0.17351210791475324 1105 5 -0.19847131721958533 1105 6 -0.26710351800805654 1105 7 -0.267422165669835 1105 8 -0.2965549248390644 1105 9 -0.4646256254384724 1105 10-11 -0.45368445752338005 1105 12-13 -0.4751162861786824 1105 14-15 -0.6622151042117537 1105 16-17 -0.6188460821087105 1105 18-19 -0.5860302541577482 1105 20-21 -0.6699594735417733 1105 22-23 -0.5983185200826782 1105 24-25 -0.664402627207707 1105 26-27 -0.6941839022378886 1105 28-29 -0.6987309852106378 1105 30-31 -0.6957420228141586 1105 32-33 -0.634480375094391 1105 34-35 -0.6400057435457072 1105 36-37 -0.7495096581781624 1105 38-39 -0.7262983396183813 1105 40-41 -0.7002536290309678 1105 42-43 -0.6917834525684654 1105 44-45 -0.7297049607033159 1105 46-47 -0.8300159803113658 1105 48-49 -0.7377738145852675 1105 50-51 -0.7996680489748158 1105 52-53 -0.7791332849190269 1105 54-55 -0.8159894109848338 1105 56-57 -0.8725642501873523 1105 58-59 -0.8302111158311973 1105 60-61 -0.8692244305401715 1105 62-63 -0.8346571419846427 1105 64-65 -0.843951814113062 1105 66-67 -0.9091482878121013 1105 68-69 -0.9228724359434608 1105 70-71 -0.8933836467867025 1105 72-73 -0.896857941818844 1105 74-75 -0.8861780127669476 1105 76-77 -0.9002004938464552 1105 78-79 -0.8184154995732307 1105 80-81 -0.71498274081641 1105 82-83 -0.7739187039050179 1105 84-85 -0.6202863863724666 1105 86-87 -0.6652612166227492 1105 88-89 -0.7179554335777958 1105 90-91 -0.7199431243377639 1105 92-93 -0.7661695960390702 1105 94-95 -0.752569432265819 1105 96-97 -0.8346016867089716 1105 98-99 -0.8820628544100728 1105 100 -0.9646376431630195 1106 1 -0.34068100070887297 1106 2 -0.3128048294552528 1106 3 -0.35755657168570565 1106 4 -0.1950319363032449 1106 5 -0.2714816651516827 1106 6 -0.2848524953771516 1106 7 -0.3140245134706632 1106 8 -0.35445230031152875 1106 9 -0.4669345045287159 1106 10-11 -0.47226833735525986 1106 12-13 -0.5388854954267046 1106 14-15 -0.6678363005083696 1106 16-17 -0.6730582111079286 1106 18-19 -0.6836271286115547 1106 20-21 -0.6563584906235889 1106 22-23 -0.6535606728176191 1106 24-25 -0.7406324024947821 1106 26-27 -0.7394950040679831 1106 28-29 -0.7532353630663451 1106 30-31 -0.7568139840427861 1106 32-33 -0.7151193155134692 1106 34-35 -0.7064681413102321 1106 36-37 -0.7539993564236411 1106 38-39 -0.8146536629567507 1106 40-41 -0.765207447177545 1106 42-43 -0.7477522386442459 1106 44-45 -0.7915813280421347 1106 46-47 -0.8250889339676561 1106 48-49 -0.8336028270864446 1106 50-51 -0.8222129710714015 1106 52-53 -0.8267777613157818 1106 54-55 -0.8281802616489529 1106 56-57 -0.8736222102553342 1106 58-59 -0.8580161417373304 1106 60-61 -0.9284024510246311 1106 62-63 -0.8656632877126071 1106 64-65 -0.9231211496770229 1106 66-67 -0.791965930729809 1106 68-69 -0.8758430964902715 1106 70-71 -0.8247007259843997 1106 72-73 -0.8137456417401623 1106 74-75 -0.8086058912645555 1106 76-77 -0.849817362795477 1106 78-79 -0.690493128109452 1106 80-81 -0.6479098165016595 1106 82-83 -0.7209473392789647 1106 84-85 -0.7171669780394119 1106 86-87 -0.6807274321170098 1106 88-89 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1.1135418059639797 2206 92-93 1.1678269564430366 2206 94-95 1.1869770212795778 2206 96-97 1.17041694890316 2206 98-99 1.1883305658855257 2206 100 1.430971156204528 2207 1 0.6721187100584558 2207 2 0.6202428718800945 2207 3 0.5930021425550436 2207 4 0.3723076860712595 2207 5 0.4660879686170176 2207 6 0.47306210832818607 2207 7 0.5181514861533714 2207 8 0.5176315837564331 2207 9 0.7019387359882217 2207 10-11 0.6826427319240622 2207 12-13 0.683943800645956 2207 14-15 0.8600960751151092 2207 16-17 0.8604353250384165 2207 18-19 0.8612571214252043 2207 20-21 0.8406888532729582 2207 22-23 0.8524105177607026 2207 24-25 0.8836456900099847 2207 26-27 0.8799616143981339 2207 28-29 0.9293690751892996 2207 30-31 0.9485074458896392 2207 32-33 1.0089653010390123 2207 34-35 0.9587827744850514 2207 36-37 0.9595728121393137 2207 38-39 0.9912810031371464 2207 40-41 1.0386690144187725 2207 42-43 1.118533817255475 2207 44-45 1.0944846286799716 2207 46-47 1.0739305035726048 2207 48-49 1.0739979841945697 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2311 30-31 0.8430785372534544 2311 32-33 1.0211161492863212 2311 34-35 0.988193551085935 2311 36-37 1.0424672821863297 2311 38-39 1.0582395297607832 2311 40-41 1.0276620052576462 2311 42-43 1.0971988706731892 2311 44-45 1.054650124153106 2311 46-47 1.0940461126393757 2311 48-49 1.0899869335802492 2311 50-51 1.0878721513980523 2311 52-53 1.0863507687029426 2311 54-55 1.1033541821849653 2311 56-57 1.1036030283623646 2311 58-59 1.1148218620773847 2311 60-61 1.1158227992401066 2311 62-63 1.1311242420401086 2311 64-65 1.134665065666148 2311 66-67 1.193147476620858 2311 68-69 1.1708237503523975 2311 70-71 1.2218217049804565 2311 72-73 1.2191811479246937 2311 74-75 1.1115753358454086 2311 76-77 1.046729774537667 2311 78-79 0.9817001544628923 2311 80-81 1.0231494310124702 2311 82-83 1.0434537581354135 2311 84-85 1.0660794771087545 2311 86-87 1.1144813840522119 2311 88-89 1.134477153914073 2311 90-91 1.1735139439667641 2311 92-93 1.2292662752446262 2311 94-95 1.2943833258037962 2311 96-97 1.329349618967825 2311 98-99 1.3393037719339596 2311 100 1.4510425444805826 2312 1 0.10907065022224316 2312 2 0.10353752900066837 2312 3 0.31216527229407376 2312 4 0.2311408017170038 2312 5 0.3143996792447581 2312 6 0.4010803277858841 2312 7 0.4380740216971404 2312 8 0.2648716101776145 2312 9 0.3991934381671527 2312 10-11 0.2713244900537646 2312 12-13 0.45485919851641654 2312 14-15 0.7819098386202725 2312 16-17 0.8292143776677747 2312 18-19 0.8605606410556277 2312 20-21 0.8536211994623883 2312 22-23 0.4734401831633335 2312 24-25 0.43411435448255986 2312 26-27 0.7690551174665003 2312 28-29 0.9212770041899532 2312 30-31 0.8707339611677369 2312 32-33 0.696157252394336 2312 34-35 0.8962983226838759 2312 36-37 0.9747382203826618 2312 38-39 0.9725692070761198 2312 40-41 1.0034325363882601 2312 42-43 0.8839774967727791 2312 44-45 0.8488958465429732 2312 46-47 0.9918799219030348 2312 48-49 1.0571551627042481 2312 50-51 1.080848912015533 2312 52-53 1.0923230695451451 2312 54-55 1.1039265617126262 2312 56-57 1.059264618823974 2312 58-59 1.1033195184274902 2312 60-61 1.1244225742099516 2312 62-63 1.107552339487789 2312 64-65 1.1495730540154554 2312 66-67 1.1538120602720099 2312 68-69 1.161329416717436 2312 70-71 1.00175382656888 2312 72-73 1.11875455694814 2312 74-75 1.1625035875107343 2312 76-77 1.1401640253312664 2312 78-79 1.019909419677127 2312 80-81 0.8708794904066508 2312 82-83 0.9195151705781157 2312 84-85 0.7649650578848473 2312 86-87 0.9343169827543036 2312 88-89 0.5743559937492897 2312 90-91 0.9604510124215615 2312 92-93 1.1203352431852522 2312 94-95 1.1544217110742743 2312 96-97 1.2418342240888443 2312 98-99 1.2538169938197292 2312 100 1.3414524859329013 2313 1 -0.3315793260839328 2313 2 -0.005374954973724755 2313 3 0.26147369443581425 2313 4 0.09089920429334342 2313 5 0.22805631712349594 2313 6 0.34987923913100616 2313 7 0.11394051730801635 2313 8 0.09396388466773686 2313 9 0.28370417553327343 2313 10-11 0.36776336866128645 2313 12-13 0.5417128801638924 2313 14-15 0.6051171245309561 2313 16-17 0.7381310318981278 2313 18-19 0.6024101931346095 2313 20-21 0.7966874087986042 2313 22-23 0.8557432281847355 2313 24-25 0.7092604109070848 2313 26-27 0.8768135359497151 2313 28-29 0.7568285015218379 2313 30-31 0.7559407274724563 2313 32-33 0.9215101681832536 2313 34-35 0.9255858345364416 2313 36-37 0.78300692938938 2313 38-39 0.5871910653938244 2313 40-41 0.7645892413497251 2313 42-43 0.7883308524603976 2313 44-45 0.9553208511991613 2313 46-47 1.0300030610894524 2313 48-49 1.0405420386772661 2313 50-51 0.9764937057315066 2313 52-53 0.9259097834364951 2313 54-55 0.9899779428914215 2313 56-57 1.0733690134655873 2313 58-59 0.9861795940807312 2313 60-61 1.015143640914694 2313 62-63 1.0461635308072132 2313 64-65 1.101194370277966 2313 66-67 1.135238316367527 2313 68-69 1.1546080901929798 2313 70-71 1.0268025746970295 2313 72-73 1.1043237701988673 2313 74-75 0.7687522640177846 2313 76-77 0.9430593341536095 2313 78-79 0.7841447127803391 2313 80-81 0.9219776724042816 2313 82-83 0.914166037858898 2313 84-85 0.9812597867454507 2313 86-87 1.0338966296541514 2313 88-89 1.0061485767407383 2313 90-91 1.1018569406247458 2313 92-93 0.876343313436486 2313 94-95 0.9086198166157295 2313 96-97 1.1367344204712566 2313 98-99 1.2287910964855833 2313 100 1.3151832756142348 2314 1 -0.37295765366940614 2314 2 -0.18116826401342934 2314 3 0.1180414527303455 2314 4 -0.14931094345215 2314 5 0.1815499771964184 2314 6 0.20377246375616664 2314 7 0.11195744297320687 2314 8 0.1890073910019865 2314 9 0.22683154460298027 2314 10-11 0.3914403171494598 2314 12-13 0.4843678887776477 2314 14-15 0.5741975179500898 2314 16-17 0.7615932208805205 2314 18-19 0.797792124842637 2314 20-21 0.4618954150160093 2314 22-23 0.6440064859794035 2314 24-25 0.8438172204769998 2314 26-27 0.8279044186368552 2314 28-29 0.7382996625207383 2314 30-31 0.7289580393282336 2314 32-33 0.8620603007561343 2314 34-35 0.8782053435981325 2314 36-37 0.790220640192544 2314 38-39 0.8243695504471162 2314 40-41 0.8606765668121881 2314 42-43 0.6892152786711776 2314 44-45 0.7278611329441063 2314 46-47 0.8163933177060301 2314 48-49 0.721275414884154 2314 50-51 0.7997448667966793 2314 52-53 0.8927429204152446 2314 54-55 0.9404911339889779 2314 56-57 0.8140166597715393 2314 58-59 0.7644067340919563 2314 60-61 0.8298542551604484 2314 62-63 0.5480794414449477 2314 64-65 0.7293001512263473 2314 66-67 0.8364534561111938 2314 68-69 0.9191523443113567 2314 70-71 0.9379660854806744 2314 72-73 0.9531197797867108 2314 74-75 0.9562340559958287 2314 76-77 0.7753142666860526 2314 78-79 0.7631679342688003 2314 80-81 0.7696665760359735 2314 82-83 0.7467290685822121 2314 84-85 0.6561827808339125 2314 86-87 0.7977909219010542 2314 88-89 0.8884698815448182 2314 90-91 0.9441025785198711 2314 92-93 0.9788722270040608 2314 94-95 1.0537246267253444 2314 96-97 1.1485792976707856 2314 98-99 1.1333907685574118 2314 100 1.1799418055139448 2315 1 -0.10707024201811777 2315 2 0.09318440662762129 2315 3 0.059504742279017364 2315 4 -0.08851055147318476 2315 5 0.21844971096390253 2315 6 0.3564170098991539 2315 7 0.3818483898406413 2315 8 0.35756216254708306 2315 9 0.562667435850237 2315 10-11 0.5348842738982214 2315 12-13 0.6053049634385488 2315 14-15 0.6810755841617535 2315 16-17 0.7002558957183354 2315 18-19 0.7808603204489586 2315 20-21 0.7654160148186051 2315 22-23 0.823872281681524 2315 24-25 0.7573337577301302 2315 26-27 0.8447472406922358 2315 28-29 0.8842150754414817 2315 30-31 0.8316164045071517 2315 32-33 0.8492604746707499 2315 34-35 0.8491142884017648 2315 36-37 0.8723187037249218 2315 38-39 0.8909570211710758 2315 40-41 0.8605788368577123 2315 42-43 0.9349780971462494 2315 44-45 0.8241517052334757 2315 46-47 0.8078317036169054 2315 48-49 0.9029672513423392 2315 50-51 0.8030713622492343 2315 52-53 0.8240544941927084 2315 54-55 0.725214685170819 2315 56-57 0.8660861696803934 2315 58-59 0.8914596681525708 2315 60-61 0.9601741451390282 2315 62-63 0.948493981970536 2315 64-65 0.9676802255331651 2315 66-67 0.9863951094577104 2315 68-69 0.8770640172188209 2315 70-71 0.593944349755624 2315 72-73 0.8123813288631467 2315 74-75 0.9446491944681554 2315 76-77 0.9142281910701868 2315 78-79 0.8085237398850396 2315 80-81 0.7981048537754987 2315 82-83 0.7628815311275545 2315 84-85 0.713372468275633 2315 86-87 0.7115553457995389 2315 88-89 0.8130775197370674 2315 90-91 0.8770143335702336 2315 92-93 0.9229673028074359 2315 94-95 0.9912472545491227 2315 96-97 1.0360988281845138 2315 98-99 0.9104651243615436 2315 100 0.9056200516928001 2316 1 0.5008470459771317 2316 2 0.47702521078289806 2316 3 0.47367546411666694 2316 4 0.2919749133460172 2316 5 0.39121321595693104 2316 6 0.39542646779453605 2316 7 0.45597463821530937 2316 8 0.4567349553972875 2316 9 0.5140179531330347 2316 10-11 0.48166837056331957 2316 12-13 0.6099458667796753 2316 14-15 0.7697354792060125 2316 16-17 0.749844848443864 2316 18-19 0.7709526258974435 2316 20-21 0.8123110308914434 2316 22-23 0.8391162176680638 2316 24-25 0.9072095470403525 2316 26-27 0.9487231557381293 2316 28-29 0.8848678166546264 2316 30-31 0.7638180253336131 2316 32-33 0.755946358447737 2316 34-35 0.681633410447489 2316 36-37 0.6683694663207902 2316 38-39 0.6201819106054671 2316 40-41 0.697334795254875 2316 42-43 0.8490805589713446 2316 44-45 0.7900317021375614 2316 46-47 0.8221181675285152 2316 48-49 0.6185650804797831 2316 50-51 0.7081720541828247 2316 52-53 0.8626094312506751 2316 54-55 0.9032560058551482 2316 56-57 0.9342145153463193 2316 58-59 0.864327724962223 2316 60-61 0.9325114761936177 2316 62-63 0.9456974685393291 2316 64-65 0.9523463201353266 2316 66-67 0.938767260562976 2316 68-69 0.9309472742975231 2316 70-71 0.771950997568112 2316 72-73 0.8271236004691289 2316 74-75 0.8867774235089669 2316 76-77 0.7280850836713384 2316 78-79 0.7343457969514517 2316 80-81 0.6939911745106073 2316 82-83 0.6442606319064268 2316 84-85 0.6968034283415712 2316 86-87 0.6795654775465039 2316 88-89 0.6337360521199678 2316 90-91 0.722287184083303 2316 92-93 0.7586394098508222 2316 94-95 0.6566651728126054 2316 96-97 0.8232520876466474 2316 98-99 0.8936752918145281 2316 100 0.5235305135823154 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 3.0 11 20.0 12 66.0 13 399.0 14 1577.0 15 4709.0 16 11438.0 17 23641.0 18 40647.0 19 59877.0 20 87061.0 21 121103.0 22 145997.0 23 146679.0 24 149795.0 25 166801.0 26 199589.0 27 246188.0 28 305126.0 29 372815.0 30 456821.0 31 569859.0 32 724272.0 33 952526.0 34 1348593.0 35 1869607.0 36 2862549.0 37 4295823.0 38 3889712.0 39 704698.0 40 48.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.29006060551433 22.06562701714762 14.192961439442112 23.451350937895945 2 14.709132556582794 21.21235924336212 41.13546091975327 22.94304728030182 3 17.101139718658242 28.45437962096836 30.879135039447753 23.565345620925644 4 11.76732480905002 18.325862588099415 40.780579990632965 29.1262326122176 5 12.339669955490688 39.09240442503685 35.5559847658683 13.011940853604157 6 28.120328957780572 39.87496328709173 18.86657566234945 13.138132092778246 7 25.271263497631818 33.41181798585616 24.430520635106696 16.886397881405323 8 25.421857695270862 35.501498484206316 20.875585067815177 18.20105875270764 9 26.46422388811425 15.249400698455878 21.765162267064753 36.52121314636512 10-11 24.40217407120836 26.784086588313894 28.752847126049186 20.06089221442856 12-13 25.069433988047862 24.705198786818993 28.780178636727697 21.445188588405443 14-15 22.621110041138458 25.803268832396707 26.495997185144972 25.079623941319866 16-17 21.3663701094768 28.383661795286535 27.553462273537864 22.6965058216988 18-19 20.985447999166315 28.195498045124822 29.69783843426972 21.121215521439147 20-21 22.33194269248509 27.164920280288978 28.975942802415037 21.5271942248109 22-23 22.494517610593544 27.04138895583071 28.672454370655707 21.791639062920044 24-25 21.965357765334897 27.372179632767878 29.201477212722875 21.46098538917435 26-27 21.85037773997375 27.54653347124178 28.737534945274923 21.86555384350955 28-29 21.314476750215448 27.957025057244365 28.916742868488804 21.81175532405138 30-31 22.21414498546072 27.3794116436818 28.76052043498442 21.645922935873056 32-33 22.396437932133185 27.737254265843493 28.159835252032668 21.706472549990657 34-35 22.276742008155423 27.48037168740031 28.974994331378863 21.267891973065407 36-37 22.05219558708619 27.425483514579685 28.860342716750125 21.661978181584004 38-39 22.118028140223338 27.131387206065245 29.25930802575134 21.491276627960076 40-41 21.838957186636126 26.595035138401823 29.65648924312379 21.90951843183826 42-43 21.729002277369833 26.839857887020578 29.07816692163528 22.352972913974302 44-45 21.58416299145947 27.570459630323484 28.942005501206747 21.903371877010304 46-47 21.695244017278174 27.10703217503817 28.584261064372402 22.613462743311256 48-49 22.144271079898147 26.807125169643342 28.75245148642061 22.2961522640379 50-51 21.86855872639534 27.00237574658096 28.21862284649721 22.910442680526497 52-53 21.20086637445828 27.42013909321751 28.395993619252092 22.983000913072118 54-55 21.32996032148762 27.351885370914292 28.245402303185056 23.072752004413033 56-57 21.918762186874048 26.93324661488023 28.052408145292212 23.09558305295351 58-59 21.297963921789037 27.137508571900604 28.62990000411006 22.934627502200296 60-61 21.52961965668551 27.60659500335451 27.844489409719458 23.019295930240517 62-63 21.62227268039835 27.09894238172573 27.669584285223518 23.609200652652397 64-65 20.850343408430902 27.110716728877797 27.988564805136797 24.050375057554504 66-67 21.42686409679181 26.882859899688892 27.72895428574221 23.961321717777086 68-69 21.019908632178545 27.08855646686378 27.819175443176825 24.072359457780852 70-71 21.155807527536332 26.77753231271009 27.858712889549203 24.20794727020438 72-73 21.50028330786419 27.125191898324214 27.658670442180778 23.71585435163082 74-75 21.472444585469862 27.423763055697346 27.402800783357662 23.70099157547513 76-77 21.258500473923476 27.327156144242437 27.251827574681297 24.162515807152786 78-79 21.100044068842962 27.3247504208205 27.325294574348934 24.2499109359876 80-81 20.953668147327686 27.683495433685145 27.392961914384717 23.969874504602455 82-83 21.44981910814018 27.06035248662581 27.81646279195616 23.673365613277852 84-85 21.767679685709105 27.24308471871215 26.86771371896832 24.121521876610423 86-87 20.89095034441777 27.68731755441292 27.19999422731687 24.22173787385244 88-89 21.383268674964246 26.947968575751553 27.121214127759746 24.547548621524456 90-91 21.344145211581537 26.81426949407424 27.30763103502232 24.533954259321895 92-93 21.444378571254816 27.132528917651012 26.994018027552176 24.429074483542003 94-95 20.669383359544543 26.9419783006567 27.40628886300767 24.98234947679109 96-97 20.908541446331615 26.83493615314287 27.22360231581057 25.03292008471495 98-99 20.58377780690888 27.2712308835273 27.100041934104052 25.044949375459773 100 20.96904749273372 26.92381249941785 26.800310049516398 25.306829958332035 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 679.0 1 506.0 2 493.5 3 724.0 4 688.0 5 737.5 6 1205.5 7 2296.5 8 3889.5 9 4967.5 10 5094.5 11 5359.0 12 6395.0 13 7750.5 14 9389.0 15 11577.0 16 13967.0 17 16340.5 18 18697.0 19 20957.0 20 23526.5 21 26484.5 22 30426.0 23 35980.5 24 43802.0 25 53784.0 26 67494.0 27 84609.5 28 108783.5 29 149144.0 30 200376.0 31 250999.5 32 319159.0 33 390013.5 34 453461.5 35 515589.5 36 579067.0 37 675127.0 38 715861.5 39 681877.5 40 651532.0 41 630459.5 42 631029.0 43 652760.0 44 689265.5 45 762141.5 46 821830.0 47 861272.5 48 890200.5 49 867631.0 50 822479.0 51 794350.5 52 763675.0 53 717090.0 54 673432.5 55 596010.5 56 515691.5 57 426388.5 58 335469.5 59 289629.5 60 232274.5 61 167968.5 62 124963.5 63 97235.5 64 69959.5 65 41940.5 66 26691.0 67 19305.5 68 14153.5 69 10042.0 70 7431.0 71 5252.0 72 3597.0 73 2630.0 74 1938.5 75 1297.0 76 810.5 77 476.5 78 278.5 79 175.0 80 105.0 81 66.5 82 45.0 83 32.5 84 20.0 85 17.5 86 15.5 87 12.5 88 8.0 89 3.0 90 4.0 91 3.0 92 2.0 93 1.5 94 0.5 95 1.0 96 1.5 97 0.5 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05145247461046108 2 5.061231026014272E-6 3 2.530615513007136E-5 4 0.018478554475978105 5 0.004175515596461774 6 1.0122462052028544E-5 7 1.822043169365138E-4 8 3.6946986489904185E-4 9 0.002307921347862508 10-11 8.93307276091519E-4 12-13 3.239187856649134E-4 14-15 2.7836770643078498E-5 16-17 8.857154295524976E-5 18-19 0.0 20-21 1.0122462052028544E-5 22-23 1.265307756503568E-5 24-25 9.11021584682569E-5 26-27 1.5183693078042815E-5 28-29 0.0010122462052028543 30-31 9.211440467345976E-4 32-33 0.014032263019624569 34-35 0.023069091016573052 36-37 0.030878570489713073 38-39 0.02043725088304563 40-41 1.3159200667637105E-4 42-43 0.0019156759433464018 44-45 0.010173074362288686 46-47 0.014890141678533989 48-49 0.005810293217864384 50-51 0.0108512793197746 52-53 0.016709654232386118 54-55 0.01015282943818463 56-57 0.003107595849972763 58-59 0.00823462287932522 60-61 0.001637308236915617 62-63 0.0010198380517418759 64-65 0.019928597164931196 66-67 0.04645450897227199 68-69 0.05644537901762416 70-71 0.049658268211739034 72-73 0.04212209521400378 74-75 0.029792936434633013 76-77 0.029886569208614277 78-79 0.013083282202246894 80-81 0.002135839492978023 82-83 0.02849473067646035 84-85 0.04914455326259858 86-87 0.049886023607909676 88-89 0.061127017716687375 90-91 0.03597269951739644 92-93 0.027535627397030644 94-95 0.007806948857627014 96-97 0.0036618006473213257 98-99 0.022408600367678187 100 0.019106147123203875 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.9758039E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.01101796576906 #Duplication Level Percentage of deduplicated Percentage of total 1 66.81046774668523 32.07638567287539 2 16.076351554981844 15.436840066605054 3 6.709003627991102 9.663182811476718 4 3.5097078068377967 6.740185782747575 5 2.0303573244903586 4.873976099151871 6 1.2766542388830917 3.67760817594548 7 0.8472884621624065 2.8475427105347695 8 0.5595498551364275 2.14916465181588 9 0.4243488526339661 1.8336078348806553 >10 1.658074016167619 12.95743098401125 >50 0.06348939373830179 2.0772823724007594 >100 0.031246522197737145 2.771394599694039 >500 0.0023218029256362907 0.754229227885007 >1k 0.001018015843083478 0.8677329304365721 >5k 5.1762568015061815E-5 0.1793549774797316 >10k+ 6.901675735341576E-5 1.0940811020590544 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 49967 0.25289453067685513 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 37677 0.19069200136713974 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23831 0.12061419658094613 No Hit CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT 20598 0.10425123667384198 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 5.061231026014272E-6 5.061231026014272E-6 2 0.0 0.0 0.0 1.5183693078042816E-5 5.061231026014272E-6 3 0.0 0.0 0.0 1.5183693078042816E-5 5.061231026014272E-6 4 0.0 0.0 0.0 1.5183693078042816E-5 5.061231026014272E-6 5 0.0 0.0 0.0 2.024492410405709E-5 5.061231026014272E-6 6 0.0 0.0 0.0 2.530615513007136E-5 2.024492410405709E-5 7 0.0 0.0 0.0 3.0367386156085633E-5 2.024492410405709E-5 8 0.0 0.0 0.0 3.54286171820999E-5 2.024492410405709E-5 9 0.0 0.0 0.0 5.567354128615699E-5 2.024492410405709E-5 10-11 0.0 0.0 0.0 7.591846539021408E-5 3.0367386156085633E-5 12-13 0.0 0.0 0.0 9.363277398126402E-5 6.32653878251784E-5 14-15 0.0 0.0 0.0 1.0881646705930685E-4 9.869400500727831E-5 16-17 0.0 0.0 0.0 1.2400016013734968E-4 1.1640831359832826E-4 18-19 0.0 0.0 0.0 1.822043169365138E-4 1.4424508424140675E-4 20-21 0.0 0.0 0.0 2.9102078399582064E-4 1.467756997544139E-4 22-23 0.0 0.0 0.0 6.70613110946891E-4 1.5436754629343527E-4 24-25 5.061231026014272E-6 0.0 0.0 0.001730941010896881 1.5689816180644242E-4 26-27 5.061231026014272E-6 0.0 0.0 0.00402873989670736 1.7208185488448526E-4 28-29 5.061231026014272E-6 0.0 0.0 0.012622710178879594 1.7208185488448526E-4 30-31 5.061231026014272E-6 0.0 0.0 0.04771475549774955 1.7461247039749238E-4 32-33 5.061231026014272E-6 0.0 0.0 0.13132376143199231 1.7967370142350666E-4 34-35 5.061231026014272E-6 0.0 0.0 0.25456979814646585 1.9232677898854233E-4 36-37 5.061231026014272E-6 0.0 0.0 0.4536659736323023 1.9232677898854233E-4 38-39 5.061231026014272E-6 0.0 0.0 0.7717618130017863 2.0244924104057088E-4 40-41 5.061231026014272E-6 0.0 0.0 1.124896554764367 2.0244924104057088E-4 42-43 5.061231026014272E-6 0.0 0.0 1.4789625630357346 2.0244924104057088E-4 44-45 5.061231026014272E-6 0.0 0.0 1.8833169627815798 2.0244924104057088E-4 46-47 5.061231026014272E-6 0.0 0.0 2.353791284651275 2.0244924104057088E-4 48-49 5.061231026014272E-6 0.0 0.0 2.867093743463104 2.0244924104057088E-4 50-51 5.061231026014272E-6 0.0 0.0 3.4059503577252785 2.1257170309259944E-4 52-53 5.061231026014272E-6 0.0 0.0 3.921697391122672 2.176329341186137E-4 54-55 5.061231026014272E-6 0.0 0.0 4.4545842833896625 2.176329341186137E-4 56-57 5.061231026014272E-6 0.0 0.0 5.053095097139954 2.2269416514462796E-4 58-59 5.061231026014272E-6 0.0 0.0 5.727891315529845 2.2775539617064225E-4 60-61 5.061231026014272E-6 0.0 0.0 6.4148015903805025 2.3787785822267077E-4 62-63 1.0122462052028544E-5 0.0 0.0 7.098272758748983 2.6318401335274216E-4 64-65 1.0122462052028544E-5 0.0 0.0 7.760309613722294 2.6824524437875645E-4 66-67 1.0122462052028544E-5 0.0 0.0 8.46501011562939 2.7583709091777783E-4 68-69 1.0122462052028544E-5 0.0 0.0 9.220808805975128 2.78367706430785E-4 70-71 1.0122462052028544E-5 2.530615513007136E-6 0.0 10.023471965006244 2.834289374567992E-4 72-73 1.0122462052028544E-5 5.061231026014272E-6 0.0 10.822994630185718 3.036738615608563E-4 74-75 1.0122462052028544E-5 1.0122462052028544E-5 0.0 11.575055601418743 3.2138817015190627E-4 76-77 1.0122462052028544E-5 1.0122462052028544E-5 0.0 12.369729607275296 3.34041247716942E-4 78-79 1.0122462052028544E-5 1.0122462052028544E-5 0.0 13.200859660212231 3.441637097689705E-4 80-81 1.0122462052028544E-5 1.0122462052028544E-5 0.0 14.072937602765133 3.517555563079919E-4 82-83 1.0122462052028544E-5 1.5183693078042816E-5 0.0 14.949451714312335 3.821229424640775E-4 84-85 1.0122462052028544E-5 1.771430859104995E-5 0.0 15.768460118941967 3.922454045161061E-4 86-87 1.0122462052028544E-5 2.024492410405709E-5 0.0 16.591353018384062 4.200821751591846E-4 88 1.0122462052028544E-5 2.024492410405709E-5 0.0 17.21440068014847 4.453883302892559E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 31715 0.0 51.798244 1 GTATCAA 60895 0.0 43.392185 1 CAACGCA 73780 0.0 34.98806 5 ATCAACG 74350 0.0 34.681297 3 TATCAAC 78100 0.0 33.6532 2 AACGCAG 81235 0.0 32.61503 6 TCAACGC 83905 0.0 30.933775 4 ACGCAGA 88605 0.0 28.990103 7 CGCAGAG 89580 0.0 28.55918 8 GTACTAG 12440 0.0 26.116472 1 GCAGAGT 97080 0.0 25.781586 9 GCTATTG 21640 0.0 25.294584 8 AGGGCTA 22600 0.0 24.698915 5 GTGGTAT 10930 0.0 24.648546 1 ATTTAGG 23335 0.0 24.480782 1 CTATTGA 22705 0.0 24.211464 9 GGGCTAT 24025 0.0 24.15252 6 GTACATG 85000 0.0 24.150251 1 TACATGG 85095 0.0 23.718742 2 ACATGGG 83800 0.0 23.210941 3 >>END_MODULE