FastQCFastQC Report
Mon 6 Jun 2016
ERR1211425_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211425_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13308282
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT302320.22716681236541275No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209490.15741325589583988No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147860.11110374727556871No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA178300.045.7686841
GTATCAA331250.043.524861
TACCTGG109650.034.8531232
CAACGCA407850.034.301945
ATCAACG408600.034.22763
TATCAAC425100.033.5463942
AACGCAG441700.032.1632426
TCAACGC436500.032.1042334
ACGCAGA486550.028.7154547
GTACATG465600.028.2232761
CGCAGAG499600.027.8127388
TACATGG471950.027.3741512
ACCTGGG140100.026.6757643
ACATGGG472700.026.2645913
GTACTAG55250.025.668831
CATGGGG353100.024.6163584
GCAGAGT553100.024.2718689
GTACCTG172750.022.8013251
ATGGGGG257850.022.1328725
AGAGTAC497250.021.24109810-11
Produced by FastQC (version 0.11.5)