FastQCFastQC Report
Mon 6 Jun 2016
ERR1211425_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211425_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13308282
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT273610.20559377987331498No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199100.14960608739730644No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142050.10673804477542631No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA177500.045.1366651
GTATCAA324100.040.9679151
TACCTGG105450.033.1565322
ATCAACG393800.032.7222143
CAACGCA394600.032.627765
TATCAAC417950.031.381832
TCAACGC422000.030.569664
AACGCAG429850.030.4754456
ACGCAGA477950.026.9660647
GTACATG455400.026.9157351
GTACTAG55650.026.5291121
TACATGG459050.026.1970882
CGCAGAG490150.026.1223168
ACCTGGG133550.025.547223
ACATGGG463000.024.8981723
CATGGGG340000.022.958924
GCAGAGT550850.022.6978179
TGGGGGG224700.021.4963266
CTAACGC24950.021.0959173
ATGGGGG247000.020.9261595
Produced by FastQC (version 0.11.5)