FastQCFastQC Report
Mon 6 Jun 2016
ERR1211424_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211424_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6852875
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81150.11841745252904802No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC78840.11504660452729694No Hit
GTACATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG77430.11298907392882548No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG83800.055.3212172
ACCTGGG98600.046.003243
GGTATCA81200.042.7006231
GTATCAA134750.039.9604071
GTACATG282650.037.4012641
CCTGGGG93000.036.6421054
TGGGGGG203250.036.1239176
TACATGG289950.036.0354042
ACATGGG296150.034.4894263
ATGGGGG197200.034.1324735
CATGGGG245350.033.6406024
GTACCTG145350.032.2817231
CAACGCA163350.031.8249425
ATCAACG163150.031.8068033
CTGGGGG71150.030.9173745
TATCAAC173450.030.445152
AACGCAG179300.030.043376
TCAACGC175350.029.7806114
GTACTAG30150.029.062841
ACGCAGA194400.026.839297
Produced by FastQC (version 0.11.5)