FastQCFastQC Report
Mon 6 Jun 2016
ERR1211424_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211424_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6852875
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74170.10823194644583478No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC72500.10579501304197143No Hit
GTACATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG71700.10462761979461174No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG85950.057.570162
ACCTGGG99200.048.9805683
GGTATCA73650.043.4029351
GTATCAA127200.040.5047871
CCTGGGG93900.038.0851944
TGGGGGG183350.035.8763476
CTGGGGG65850.035.1787765
GTACATG268700.034.745181
TACATGG274250.033.703322
GTACCTG151750.033.0226171
ATGGGGG179450.032.2594385
ACATGGG276050.032.2238773
CAACGCA156000.032.078545
ATCAACG157700.031.6749943
CATGGGG229450.031.5610684
TATCAAC167950.030.4133642
TAACGCA7750.030.318344
ATAACGC8000.029.956783
TCAACGC168250.029.9417294
AACGCAG174000.029.4062546
Produced by FastQC (version 0.11.5)