##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211424_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6852875 Sequences flagged as poor quality 0 Sequence length 100 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.10012294110136 33.0 31.0 34.0 30.0 34.0 2 32.23126060230195 33.0 31.0 34.0 30.0 34.0 3 32.314663699542166 34.0 31.0 34.0 30.0 34.0 4 35.95360998121227 37.0 35.0 37.0 35.0 37.0 5 35.811928716049835 37.0 35.0 37.0 35.0 37.0 6 35.84035663863707 37.0 35.0 37.0 35.0 37.0 7 35.76545668788647 37.0 35.0 37.0 33.0 37.0 8 35.74376214362585 37.0 35.0 37.0 33.0 37.0 9 37.3395471243821 39.0 37.0 39.0 34.0 39.0 10-11 37.30429695565729 39.0 37.0 39.0 34.0 39.0 12-13 37.200240555971035 39.0 37.0 39.0 33.0 39.0 14-15 38.41995315834595 40.0 38.0 41.0 34.0 41.0 16-17 38.38936964412747 40.0 38.0 41.0 33.0 41.0 18-19 38.366231107381935 40.0 38.0 41.0 33.5 41.0 20-21 38.313117487186034 40.0 38.0 41.0 34.0 41.0 22-23 38.21581299819419 40.0 38.0 41.0 33.5 41.0 24-25 38.10189015559163 40.0 37.0 41.0 33.0 41.0 26-27 37.99314565054813 40.0 37.0 41.0 33.0 41.0 28-29 37.911446582638675 40.0 37.0 41.0 33.0 41.0 30-31 38.00775200189702 40.0 37.0 41.0 33.0 41.0 32-33 37.8835544205899 40.0 37.0 41.0 33.0 41.0 34-35 37.80384079674589 40.0 37.0 41.0 33.0 41.0 36-37 37.697996388377135 40.0 37.0 41.0 33.0 41.0 38-39 37.602154570162156 40.0 37.0 41.0 32.0 41.0 40-41 37.553082611312774 40.0 37.0 41.0 32.0 41.0 42-43 37.45075067033909 40.0 36.0 41.0 32.0 41.0 44-45 37.34069963336556 40.0 36.0 41.0 31.5 41.0 46-47 37.176434197325946 39.0 36.0 41.0 31.0 41.0 48-49 37.00950010032285 39.0 35.5 41.0 31.0 41.0 50-51 36.84941276471554 39.0 35.0 41.0 31.0 41.0 52-53 36.650074749648866 39.0 35.0 40.0 30.5 41.0 54-55 36.44114010543021 38.5 35.0 40.0 30.0 41.0 56-57 36.20746299910621 38.0 35.0 40.0 29.5 41.0 58-59 35.93879780384145 38.0 34.5 40.0 29.0 41.0 60-61 35.662094522372 37.5 34.0 40.0 29.0 41.0 62-63 35.33252532696131 37.0 34.0 40.0 28.0 41.0 64-65 34.972719770899076 36.5 34.0 39.0 28.0 41.0 66-67 34.61918734837568 36.0 33.5 39.0 28.0 40.5 68-69 34.246227386315965 35.5 33.0 39.0 27.0 40.0 70-71 33.87065669518268 35.0 33.0 38.0 26.0 40.0 72-73 33.509193659595425 35.0 32.5 37.0 26.0 39.5 74-75 33.14171154442479 35.0 32.0 36.5 26.0 39.0 76-77 32.136783904565604 34.0 30.5 35.5 25.5 38.0 78-79 32.63178957736716 35.0 32.0 36.0 26.0 37.5 80-81 32.551504587490655 35.0 32.0 35.0 26.0 37.0 82-83 32.2949327107236 34.5 32.0 35.0 25.5 37.0 84-85 32.038760739105854 34.0 31.5 35.0 25.0 36.0 86-87 31.81197001258596 34.0 31.0 35.0 25.0 36.0 88-89 31.62152571001222 34.0 31.0 35.0 24.5 35.5 90-91 31.416635572661107 34.0 31.0 35.0 24.0 35.0 92-93 31.242507779581562 34.0 31.0 35.0 24.0 35.0 94-95 31.091525454644948 34.0 31.0 35.0 23.0 35.0 96-97 30.934551264980026 34.0 30.0 35.0 23.0 35.0 98-99 30.752277766630794 34.0 30.0 35.0 21.5 35.0 100 28.83235153129161 32.0 26.0 34.0 18.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.2980068166008607 1101 2 -0.2308292274846977 1101 3 -0.3081102098352808 1101 4 -0.15489027550925272 1101 5 -0.24000578302554487 1101 6 -0.23510172810755847 1101 7 -0.2550900804895875 1101 8 -0.36471494848380814 1101 9 -0.5108172408865244 1101 10-11 -0.41145580845686425 1101 12-13 -0.44868312359597695 1101 14-15 -0.6052506696616362 1101 16-17 -0.6457860628928458 1101 18-19 -0.6809590955796097 1101 20-21 -0.6500245607582471 1101 22-23 -0.7650612358610687 1101 24-25 -0.7686825166089193 1101 26-27 -0.6979955141666707 1101 28-29 -0.7016318072124221 1101 30-31 -0.7027894170487201 1101 32-33 -0.6283998668429618 1101 34-35 -0.8481387771097744 1101 36-37 -0.8123632106120269 1101 38-39 -0.7761777806075258 1101 40-41 -0.8535054041031458 1101 42-43 -0.8162301603246078 1101 44-45 -0.8134400093415195 1101 46-47 -0.9972434445926694 1101 48-49 -0.9223469022639676 1101 50-51 -0.971571789455794 1101 52-53 -1.075602512729752 1101 54-55 -1.1151021372298047 1101 56-57 -1.1081289252068416 1101 58-59 -1.1010616045970707 1101 60-61 -1.0112271011567273 1101 62-63 -1.1555999608474252 1101 64-65 -1.144483083078633 1101 66-67 -1.1698944688829727 1101 68-69 -1.2795837082022459 1101 70-71 -1.193232675427403 1101 72-73 -1.2516874292221658 1101 74-75 -1.2927354345760236 1101 76-77 -1.1474912195617613 1101 78-79 -1.0273983886329603 1101 80-81 -1.004991841950698 1101 82-83 -1.0775648894352656 1101 84-85 -1.1079269764677697 1101 86-87 -1.1211428975698396 1101 88-89 -1.1998460604997767 1101 90-91 -1.3108956722072413 1101 92-93 -1.4047241609884367 1101 94-95 -1.5240559221703904 1101 96-97 -1.4866723060045572 1101 98-99 -1.7625453177794341 1101 100 -1.5874640563168647 1102 1 -0.28923932822922893 1102 2 -0.2548472195802667 1102 3 -0.28876035729606997 1102 4 -0.1510291129958361 1102 5 -0.22784363413617115 1102 6 -0.2505915392394442 1102 7 -0.29962816491458 1102 8 -0.38519139079106424 1102 9 -0.40681568908070886 1102 10-11 -0.46581635427662604 1102 12-13 -0.49784798851110423 1102 14-15 -0.6344194740461049 1102 16-17 -0.6361309387689786 1102 18-19 -0.6473349180784851 1102 20-21 -0.6992036710323717 1102 22-23 -0.7374315761023951 1102 24-25 -0.7449209166033341 1102 26-27 -0.8451530824846145 1102 28-29 -0.8416913847401588 1102 30-31 -0.7052053032001027 1102 32-33 -0.7257989933112086 1102 34-35 -0.78271604589888 1102 36-37 -0.7317120687788758 1102 38-39 -0.7496821196658843 1102 40-41 -0.7633728730333402 1102 42-43 -0.8111310407755283 1102 44-45 -0.8123164660574318 1102 46-47 -0.8444697927696652 1102 48-49 -0.8022749038555474 1102 50-51 -0.9370389432771873 1102 52-53 -0.9468994962451234 1102 54-55 -1.004228107562362 1102 56-57 -1.062527483834856 1102 58-59 -1.088904427569517 1102 60-61 -1.0992199890546246 1102 62-63 -1.037665570559632 1102 64-65 -0.9319327122082015 1102 66-67 -1.0038451660976335 1102 68-69 -1.0703082222862292 1102 70-71 -1.0649776139441158 1102 72-73 -1.1462362628611587 1102 74-75 -1.1976539512444475 1102 76-77 -1.0053460678594135 1102 78-79 -0.8743354670650803 1102 80-81 -0.8168865314831528 1102 82-83 -0.8787631544407191 1102 84-85 -0.9727272720108324 1102 86-87 -0.9966757367537262 1102 88-89 -0.9600492531808449 1102 90-91 -1.0062658232984667 1102 92-93 -1.0883139241881636 1102 94-95 -1.0774434941292554 1102 96-97 -1.1600294535811386 1102 98-99 -1.3129840760549918 1102 100 -1.3454327992452129 1103 1 -0.3110454686955073 1103 2 -0.30083086049728536 1103 3 -0.3213924412264113 1103 4 -0.16726971284627012 1103 5 -0.151414590624384 1103 6 -0.2676625596811917 1103 7 -0.32544406137881765 1103 8 -0.3422500076168902 1103 9 -0.5090769436040361 1103 10-11 -0.47395545139765716 1103 12-13 -0.4772770923411116 1103 14-15 -0.6847072674739323 1103 16-17 -0.7184323453185542 1103 18-19 -0.6948200040109285 1103 20-21 -0.7233673775840188 1103 22-23 -0.7836052029628036 1103 24-25 -0.7449018914091923 1103 26-27 -0.7568635404109116 1103 28-29 -0.8026497357022038 1103 30-31 -0.7288033965564509 1103 32-33 -0.661423372949919 1103 34-35 -0.6818647457269975 1103 36-37 -0.6382592511463585 1103 38-39 -0.7853147123111697 1103 40-41 -0.7706812835650112 1103 42-43 -0.7865330734009248 1103 44-45 -0.8429400366820516 1103 46-47 -0.7437369377312351 1103 48-49 -0.837433475302106 1103 50-51 -0.9181189323626455 1103 52-53 -0.9729067213095988 1103 54-55 -0.9210553547208846 1103 56-57 -1.0018304898940684 1103 58-59 -1.0527502887398654 1103 60-61 -1.0248180913789113 1103 62-63 -0.9645583740785497 1103 64-65 -0.9321402160234413 1103 66-67 -1.0867221924359853 1103 68-69 -1.0841266438573243 1103 70-71 -0.9708764957212281 1103 72-73 -0.975147023883359 1103 74-75 -1.072030556305208 1103 76-77 -0.9780243184119968 1103 78-79 -0.7996489641381999 1103 80-81 -0.8250575230375148 1103 82-83 -0.7791049442068498 1103 84-85 -0.8168810641816897 1103 86-87 -0.8360270987258289 1103 88-89 -1.0062508605032185 1103 90-91 -1.0546176068901545 1103 92-93 -1.0152253222377112 1103 94-95 -1.0181568218705515 1103 96-97 -1.1149095716781936 1103 98-99 -1.2563420957810543 1103 100 -1.2962248312507079 1104 1 -0.44829968055517 1104 2 -0.3430873623424979 1104 3 -0.39906309230691406 1104 4 -0.30491912261428666 1104 5 -0.25066309350751226 1104 6 -0.3674803959887498 1104 7 -0.32916255624986945 1104 8 -0.40471221020343506 1104 9 -0.47156695726573616 1104 10-11 -0.47009410559465437 1104 12-13 -0.5926836228600294 1104 14-15 -0.7670862484660219 1104 16-17 -0.7577868569351409 1104 18-19 -0.7797575349073327 1104 20-21 -0.687933330433097 1104 22-23 -0.7026433446217055 1104 24-25 -0.799234702796042 1104 26-27 -0.8787978524456506 1104 28-29 -0.8938851294244472 1104 30-31 -0.9234805776037902 1104 32-33 -0.8087483454781434 1104 34-35 -0.850140038105593 1104 36-37 -0.8082747485171851 1104 38-39 -0.9373176503016722 1104 40-41 -0.9838079730188127 1104 42-43 -0.8993050278754211 1104 44-45 -0.9331933014929419 1104 46-47 -0.9408608723013714 1104 48-49 -0.9953069885079913 1104 50-51 -0.9394228140204248 1104 52-53 -0.8769093998220825 1104 54-55 -0.9525610012432679 1104 56-57 -0.9445828063909971 1104 58-59 -1.0689160225048298 1104 60-61 -1.0601177988450488 1104 62-63 -1.0483422859351492 1104 64-65 -1.011213957698807 1104 66-67 -1.0076929622828885 1104 68-69 -0.9995224318021272 1104 70-71 -1.032473081468936 1104 72-73 -1.0279434401422378 1104 74-75 -0.9000487308083152 1104 76-77 -0.8516772697789605 1104 78-79 -0.8283414134427964 1104 80-81 -0.9347517415313504 1104 82-83 -0.9215971385880906 1104 84-85 -0.9019187747574193 1104 86-87 -0.7938775625542931 1104 88-89 -0.8919085809289911 1104 90-91 -0.8690593070324759 1104 92-93 -1.114991746011789 1104 94-95 -1.0633056525287081 1104 96-97 -1.1729781304641165 1104 98-99 -1.1186081486257784 1104 100 -1.180275421183648 1105 1 -0.40827382227779907 1105 2 -0.39183546227481614 1105 3 -0.43188138593771797 1105 4 -0.2699613786589481 1105 5 -0.24193102294606916 1105 6 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1105 68-69 -0.8397377878954089 1105 70-71 -0.8469449308054564 1105 72-73 -0.9751177945894156 1105 74-75 -0.9596039760405333 1105 76-77 -0.9743885413738553 1105 78-79 -0.9531319489411274 1105 80-81 -0.7954921928237155 1105 82-83 -0.8252186454559975 1105 84-85 -0.763602525964334 1105 86-87 -0.7721331827505402 1105 88-89 -0.8414216523992444 1105 90-91 -0.9098454925018906 1105 92-93 -0.9641578970985378 1105 94-95 -1.0161366802206615 1105 96-97 -0.9426698194258911 1105 98-99 -1.0401286944667234 1105 100 -0.9729974885693338 1106 1 -0.3407252498203732 1106 2 -0.30131822321971313 1106 3 -0.3793408132402938 1106 4 -0.19525951732933322 1106 5 -0.28309575506084883 1106 6 -0.30301204975130247 1106 7 -0.36501028976970673 1106 8 -0.3829353392463446 1106 9 -0.4475534678223738 1106 10-11 -0.43990502332025727 1106 12-13 -0.5540750198134106 1106 14-15 -0.6923240853027011 1106 16-17 -0.6855243779211833 1106 18-19 -0.6605430400615191 1106 20-21 -0.6507244070605154 1106 22-23 -0.6986996633366545 1106 24-25 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0.5667247022840805 2201 96-97 0.5859777418382208 2201 98-99 0.5379052688458792 2201 100 0.6574187709744095 2202 1 0.6277626828535006 2202 2 0.6149857959144711 2202 3 0.5766961098966163 2202 4 0.3656498175827636 2202 5 0.34913333632280796 2202 6 0.4316756973972673 2202 7 0.45238484910181853 2202 8 0.4916842768351586 2202 9 0.6938249980578988 2202 10-11 0.6350923826469028 2202 12-13 0.63959540079302 2202 14-15 0.8081031340400955 2202 16-17 0.78833923859289 2202 18-19 0.7474256711617357 2202 20-21 0.7624639774307056 2202 22-23 0.7493328638591237 2202 24-25 0.753710936997841 2202 26-27 0.7670497304547794 2202 28-29 0.7428094123260962 2202 30-31 0.8919264947200318 2202 32-33 0.5404306004992847 2202 34-35 0.838559711189788 2202 36-37 0.8994767150861378 2202 38-39 0.9506413377751741 2202 40-41 0.9451411544359587 2202 42-43 1.0031491667990693 2202 44-45 0.9201388859197408 2202 46-47 0.950072682543194 2202 48-49 1.011760232588685 2202 50-51 1.009060123076992 2202 52-53 0.9836047888131247 2202 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1.2842781296159558 2205 64-65 1.2291154468764702 2205 66-67 1.2388759485582739 2205 68-69 1.2475284363777277 2205 70-71 1.2347895317684205 2205 72-73 1.232195387345449 2205 74-75 1.2152149502228085 2205 76-77 1.2202020911746487 2205 78-79 1.0454262452120489 2205 80-81 0.9867463294240366 2205 82-83 0.99402359364516 2205 84-85 0.9913886929669964 2205 86-87 1.0801574530342108 2205 88-89 1.1082198484368497 2205 90-91 1.1960312404702016 2205 92-93 1.1674391759723761 2205 94-95 1.2327479119938296 2205 96-97 1.1923433979066118 2205 98-99 1.139265844968886 2205 100 1.3379762442059757 2206 1 0.6441947608934981 2206 2 0.6485654776239755 2206 3 0.6214524230436638 2206 4 0.24893461913717374 2206 5 0.46513731507589995 2206 6 0.5050795777899779 2206 7 0.5473640138331248 2206 8 0.5660854021514297 2206 9 0.7490714820879134 2206 10-11 0.71158063432663 2206 12-13 0.7482505242853534 2206 14-15 0.9357078874379923 2206 16-17 0.9217966182347581 2206 18-19 0.8778476595502696 2206 20-21 0.9202095583492991 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1.1752608607022488 2206 90-91 1.233629775202136 2206 92-93 1.2694713714585077 2206 94-95 1.2786331588024034 2206 96-97 1.24201725804474 2206 98-99 1.3006601233966215 2206 100 1.5057541897710465 2207 1 0.6837367031329293 2207 2 0.6340728852012418 2207 3 0.5837018274502981 2207 4 0.3552571430139153 2207 5 0.4760301385403949 2207 6 0.49848747366931434 2207 7 0.5302940233035116 2207 8 0.5518702607619801 2207 9 0.6820825186111819 2207 10-11 0.6976907542046575 2207 12-13 0.7397603954555692 2207 14-15 0.91219007971997 2207 16-17 0.8974524673337569 2207 18-19 0.8142543154133293 2207 20-21 0.8857368545925723 2207 22-23 0.9131995958586003 2207 24-25 0.9194548460495611 2207 26-27 0.9357044030467137 2207 28-29 0.9047557602560801 2207 30-31 0.9681973258001406 2207 32-33 0.990005620709205 2207 34-35 0.9629084822312848 2207 36-37 1.010441648147669 2207 38-39 1.0380713827237926 2207 40-41 1.0319255764261968 2207 42-43 1.141584599971786 2207 44-45 1.1920791377202775 2207 46-47 1.1963797861933188 2207 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1.1783833449901877 2310 74-75 1.1950764541207164 2310 76-77 1.1022535070087827 2310 78-79 0.9871054488994062 2310 80-81 1.0411093675206544 2310 82-83 1.014565763606555 2310 84-85 1.0251915361627404 2310 86-87 1.0867654887821203 2310 88-89 1.1527722975867043 2310 90-91 1.232487874878938 2310 92-93 1.2763011036316385 2310 94-95 1.2993155542681514 2310 96-97 1.3546647606474735 2310 98-99 1.3439190919281678 2310 100 1.3544970183767155 2311 1 0.333320305721422 2311 2 0.419321234889253 2311 3 0.4525102482346739 2311 4 0.28386510793901465 2311 5 -0.05527013957797067 2311 6 0.11438494824014356 2311 7 0.3283984886700395 2311 8 0.06159127124570318 2311 9 0.31636753077479085 2311 10-11 0.43295623437165887 2311 12-13 0.49197838616415623 2311 14-15 0.6028979488184021 2311 16-17 0.7961170376025208 2311 18-19 0.8021511281690721 2311 20-21 0.870012212577322 2311 22-23 0.7907292182599264 2311 24-25 0.7009760000782421 2311 26-27 0.7465017407037351 2311 28-29 0.76722996702064 2311 30-31 0.8417142878998476 2311 32-33 0.9685215621314214 2311 34-35 0.8959524285735512 2311 36-37 0.8850981609152342 2311 38-39 0.9885456076915204 2311 40-41 0.9291485209962502 2311 42-43 1.0155966556682756 2311 44-45 0.9870994992611486 2311 46-47 1.0149906376203859 2311 48-49 1.0279860756246961 2311 50-51 1.0248089868653523 2311 52-53 1.023445814727502 2311 54-55 0.9947336500919874 2311 56-57 0.9724842997020531 2311 58-59 0.9809886760306412 2311 60-61 1.03009023558824 2311 62-63 1.0608108132684748 2311 64-65 1.0940912149181372 2311 66-67 1.0636536993934556 2311 68-69 1.090083990476927 2311 70-71 1.087102012619603 2311 72-73 1.018903814135932 2311 74-75 0.962945190691002 2311 76-77 0.8900517760281232 2311 78-79 0.9125985362380291 2311 80-81 0.9220917699983957 2311 82-83 0.907771133994892 2311 84-85 0.9794993456897707 2311 86-87 1.0424454643588419 2311 88-89 1.055366490287195 2311 90-91 1.0824753930971376 2311 92-93 1.1079064235069893 2311 94-95 1.235787597355202 2311 96-97 1.2579910334474746 2311 98-99 1.2428391167420152 2311 100 1.3790738257615054 2312 1 0.10055626290622399 2312 2 0.1068378905047993 2312 3 0.3167188302181927 2312 4 0.2304070548304935 2312 5 0.2829288437190485 2312 6 0.37944983409786914 2312 7 0.42932425672283614 2312 8 0.23889637686282583 2312 9 0.3638282993012041 2312 10-11 0.2603038078615967 2312 12-13 0.4107275573302118 2312 14-15 0.7897059119392438 2312 16-17 0.8220069684540974 2312 18-19 0.8721609163033293 2312 20-21 0.8728054619221481 2312 22-23 0.45554182285088274 2312 24-25 0.46853165035768285 2312 26-27 0.8100949332142307 2312 28-29 0.9771755007896985 2312 30-31 0.8960434849570973 2312 32-33 0.7967680652415225 2312 34-35 0.9173383968094839 2312 36-37 0.9575279863075465 2312 38-39 0.9885437132139216 2312 40-41 0.984894313677259 2312 42-43 0.8782625013946515 2312 44-45 0.8429594342174127 2312 46-47 1.0538989449046454 2312 48-49 1.1041562312876039 2312 50-51 1.1499266023495522 2312 52-53 1.1427746960384866 2312 54-55 1.1648882078893408 2312 56-57 1.1466647802678764 2312 58-59 1.1000002323439944 2312 60-61 1.1386523270915134 2312 62-63 1.1679468367599952 2312 64-65 1.2593837538338661 2312 66-67 1.2390594333456448 2312 68-69 1.178836319105642 2312 70-71 1.0627702895922297 2312 72-73 1.1460033689336981 2312 74-75 1.222222289614301 2312 76-77 1.1713439676229527 2312 78-79 1.1303831025605362 2312 80-81 0.9581323118189928 2312 82-83 0.9629264811579148 2312 84-85 0.8340633000960089 2312 86-87 1.0320480976827042 2312 88-89 0.6449283923427025 2312 90-91 0.9838544149152035 2312 92-93 1.2219568166110975 2312 94-95 1.2756470902666912 2312 96-97 1.3402860351840786 2312 98-99 1.3565759738036824 2312 100 1.4652438235512797 2313 1 -0.366250808591321 2313 2 -0.08059686869115268 2313 3 0.15066448970444668 2313 4 0.050884757707393646 2313 5 0.1652261498806027 2313 6 0.28631438553921384 2313 7 0.05234032151285817 2313 8 0.04509455452893718 2313 9 0.20461619251177154 2313 10-11 0.2852069401219097 2313 12-13 0.4784863658112428 2313 14-15 0.5168085368261686 2313 16-17 0.6376425199823856 2313 18-19 0.5729039147488635 2313 20-21 0.7237527521424738 2313 22-23 0.7611922782818112 2313 24-25 0.6273609312634463 2313 26-27 0.7811214414144274 2313 28-29 0.6656863309080947 2313 30-31 0.688725882334495 2313 32-33 0.8344692030942298 2313 34-35 0.8530737907560848 2313 36-37 0.7501237442632842 2313 38-39 0.5193697814667786 2313 40-41 0.674025562599887 2313 42-43 0.720548900401262 2313 44-45 0.910802555768889 2313 46-47 0.9457599267316468 2313 48-49 1.015196171305874 2313 50-51 0.9853077349546311 2313 52-53 0.9216896476885168 2313 54-55 0.9858380761644767 2313 56-57 1.024979090912801 2313 58-59 0.9702579539232303 2313 60-61 0.917081353547843 2313 62-63 0.9810807064507685 2313 64-65 1.0799521844086755 2313 66-67 1.1448107837734653 2313 68-69 1.1512428951285116 2313 70-71 1.005221401893209 2313 72-73 1.0770304339327126 2313 74-75 0.6805565077238427 2313 76-77 0.8299896956030892 2313 78-79 0.7390153300761568 2313 80-81 0.8886128597090277 2313 82-83 0.8731179589324825 2313 84-85 0.8970090861967854 2313 86-87 0.9551569800730633 2313 88-89 0.9896217258590916 2313 90-91 1.122433174489469 2313 92-93 0.8511429216584361 2313 94-95 0.9040676350752719 2313 96-97 1.2255076868781956 2313 98-99 1.2868755468228876 2313 100 1.3148890435478329 2314 1 -0.2722916030976279 2314 2 -0.07076418153902608 2314 3 0.18550829683111658 2314 4 -0.11449583677534036 2314 5 0.2047554667951701 2314 6 0.22909021208165825 2314 7 0.1310217502663562 2314 8 0.14586623668637344 2314 9 0.2284959213771316 2314 10-11 0.42312159340207955 2314 12-13 0.502803109302981 2314 14-15 0.5745467765846186 2314 16-17 0.8000852523002138 2314 18-19 0.8537773493425718 2314 20-21 0.46955289052770155 2314 22-23 0.6788454489275892 2314 24-25 0.9299340866512864 2314 26-27 0.8977788121758223 2314 28-29 0.8171463906638508 2314 30-31 0.836985458772908 2314 32-33 0.8950820664656405 2314 34-35 0.9524634210679181 2314 36-37 0.8865369731258852 2314 38-39 0.9228965727239782 2314 40-41 0.9917579588621379 2314 42-43 0.7433246776378013 2314 44-45 0.8279094245140399 2314 46-47 0.9672387720352873 2314 48-49 0.8448306158696823 2314 50-51 1.0124379851551737 2314 52-53 1.1292234846093976 2314 54-55 1.1588761458474508 2314 56-57 1.0107844602057767 2314 58-59 0.975725298401585 2314 60-61 1.0197641099747514 2314 62-63 0.6516968927410076 2314 64-65 0.8624196413941334 2314 66-67 0.9639209736445125 2314 68-69 1.1308799992226781 2314 70-71 1.153240754010696 2314 72-73 1.160412657303084 2314 74-75 1.187979750017071 2314 76-77 0.9928696367826504 2314 78-79 1.001642302106717 2314 80-81 0.9877670917016488 2314 82-83 0.973247140390292 2314 84-85 0.8899720585118196 2314 86-87 1.0774198115973448 2314 88-89 1.1156308420717487 2314 90-91 1.2399600772078863 2314 92-93 1.258136896328054 2314 94-95 1.3393235498782623 2314 96-97 1.3781112102180373 2314 98-99 1.3669844112616936 2314 100 1.502652873068353 2315 1 -0.13335230944052512 2315 2 0.09874785542272235 2315 3 0.12178029081110964 2315 4 -0.08386760305226204 2315 5 0.2254183324847503 2315 6 0.350829282961044 2315 7 0.43134428832348704 2315 8 0.3595752553074334 2315 9 0.5436084337378873 2315 10-11 0.5454133389400369 2315 12-13 0.6523492332360448 2315 14-15 0.7510662129488566 2315 16-17 0.7085402696511665 2315 18-19 0.8011818316510642 2315 20-21 0.8155911398632796 2315 22-23 0.9267643396554632 2315 24-25 0.8038144494257295 2315 26-27 0.9473660293284212 2315 28-29 0.9757398134754851 2315 30-31 0.9294702812399933 2315 32-33 0.9536848591142117 2315 34-35 0.9117174975298639 2315 36-37 0.9284841738761784 2315 38-39 0.9853738730918025 2315 40-41 0.9679872945829686 2315 42-43 1.0551809020802239 2315 44-45 0.9070494425192877 2315 46-47 0.945679695286259 2315 48-49 1.0527212134787618 2315 50-51 0.9389132390223836 2315 52-53 0.9945940099293793 2315 54-55 0.9285651313782708 2315 56-57 1.0991206598640275 2315 58-59 1.1172047796244655 2315 60-61 1.1855298879495493 2315 62-63 1.170518942488286 2315 64-65 1.1865548258458531 2315 66-67 1.2026195950981418 2315 68-69 1.130168634790813 2315 70-71 0.7753713179524695 2315 72-73 1.0385023646756437 2315 74-75 1.1936152624859488 2315 76-77 1.1447517499533646 2315 78-79 1.0382730894382277 2315 80-81 1.0126109010372701 2315 82-83 1.046456649069853 2315 84-85 0.9662258024392791 2315 86-87 0.9714724569660618 2315 88-89 1.0601244235148748 2315 90-91 1.1754574785717757 2315 92-93 1.1993244553995623 2315 94-95 1.2503246564807746 2315 96-97 1.3445543997163654 2315 98-99 1.1931534623551379 2315 100 1.132833336419175 2316 1 0.48830004960114337 2316 2 0.48781450875992505 2316 3 0.4672816695772184 2316 4 0.3118551338193001 2316 5 0.4117844772628061 2316 6 0.3964956999875966 2316 7 0.4792303765892214 2316 8 0.4867094700513661 2316 9 0.5071798789301027 2316 10-11 0.5062274530005766 2316 12-13 0.6373171922571501 2316 14-15 0.7565704184799529 2316 16-17 0.769279735857971 2316 18-19 0.7972963656183509 2316 20-21 0.8917947054366167 2316 22-23 0.8597058040748564 2316 24-25 0.9609536253720279 2316 26-27 0.9873453341912892 2316 28-29 0.936501859329411 2316 30-31 0.8190163068055583 2316 32-33 0.7569597984924528 2316 34-35 0.6916462883323078 2316 36-37 0.6731083362244092 2316 38-39 0.6182839242234976 2316 40-41 0.7155793590722723 2316 42-43 0.9061799221924858 2316 44-45 0.8398048115772951 2316 46-47 0.8272095439423026 2316 48-49 0.6421281702507997 2316 50-51 0.7642896742016987 2316 52-53 0.9208859837266488 2316 54-55 0.9868410029569574 2316 56-57 1.040188743245551 2316 58-59 0.9855652789161908 2316 60-61 0.9602925072001014 2316 62-63 0.9918392571899588 2316 64-65 1.068425308300526 2316 66-67 1.034611163065037 2316 68-69 1.0275752646463374 2316 70-71 0.8760601408260555 2316 72-73 0.9067288799035467 2316 74-75 0.9552946567768643 2316 76-77 0.8019967141318816 2316 78-79 0.8490859969527378 2316 80-81 0.8164426901353252 2316 82-83 0.738508081828904 2316 84-85 0.808252443550149 2316 86-87 0.7942334594449392 2316 88-89 0.7684442622054171 2316 90-91 0.8263337038982606 2316 92-93 0.9855203622783364 2316 94-95 0.8770319693849906 2316 96-97 1.0659686526446066 2316 98-99 1.1350079695658302 2316 100 0.842259965636952 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 2.0 11 7.0 12 32.0 13 163.0 14 494.0 15 1511.0 16 3413.0 17 6440.0 18 10313.0 19 14459.0 20 20073.0 21 27210.0 22 34539.0 23 40695.0 24 47341.0 25 58136.0 26 72096.0 27 90095.0 28 112018.0 29 137201.0 30 169479.0 31 211600.0 32 266002.0 33 349425.0 34 499689.0 35 663614.0 36 1011305.0 37 1556627.0 38 1286672.0 39 162212.0 40 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.77794984648866 18.04614202467365 11.959605981956486 25.21630214688121 2 16.390697335060104 19.662200171460885 40.17582401546796 23.771278478011055 3 18.398353741802346 26.34755870310763 29.953622377997903 25.300465177092125 4 12.634499897909606 16.04040765518871 36.674956007724134 34.65013643917755 5 12.994093792725439 37.99102562362634 33.40173052033667 15.613150063311544 6 31.52357222333692 35.28425660762819 19.33969319446218 13.852477974572716 7 28.996160580569363 32.19555516901777 21.713346210683746 17.09493803972911 8 27.83235118707562 35.170233577304856 19.524390512617455 17.473024723002066 9 29.28803410721311 14.78261846641287 18.819483115084996 37.10986431128902 10-11 26.41985026178614 24.740149192453654 28.438630395793616 20.401370149966585 12-13 26.21832918336195 22.68373847205513 27.58033746063778 23.517594883945144 14-15 24.04344485056776 24.34318779046216 25.178414247340385 26.434953111629696 16-17 23.720918931910123 26.427821690448187 25.99344157899414 23.857817798647545 18-19 23.263943965124128 26.5389270926436 27.120580778140564 23.07654816409171 20-21 24.414940681452823 26.207057521198955 26.264923757895136 23.113078039453086 22-23 24.069474490633493 25.93337103040695 26.343359538879668 23.653794940079894 24-25 23.449178730835214 25.31296745932251 26.837992963239195 24.39986084660308 26-27 23.455932503647375 25.639250043120594 26.55202766021964 24.35278979301239 28-29 23.402577484694223 26.990772391743235 26.320886118895835 23.2857640046667 30-31 24.85017833549838 25.77012888428024 26.589332499028867 22.790360281192516 32-33 24.559417898272304 25.689741845709303 26.35343081098543 23.397409445032967 34-35 24.372114482280384 26.18483062130032 26.554495074947653 22.88855982147164 36-37 23.874026186437234 26.109505021759023 26.487218020748273 23.529250771055477 38-39 23.968235040674234 26.300077997382182 26.46901880927889 23.2626681526647 40-41 24.010240679610597 25.393669261420644 27.04210377396142 23.553986285007344 42-43 23.754074454996726 25.82835638269386 26.32113615001962 24.096433012289797 44-45 22.854393370409568 26.120335577482695 27.18847614805665 23.836794904051086 46-47 22.777759696831666 25.39926499251376 26.756654319245328 25.066320991409246 48-49 23.27449376713197 25.560388258101586 26.960263500777486 24.20485447398896 50-51 23.065612183752176 25.739666515959534 27.086690556033428 24.10803074425486 52-53 22.823199723749568 25.977114765065995 26.64153587533937 24.55814963584507 54-55 22.445686298904405 25.494061585262852 27.40795575732346 24.652296358509286 56-57 23.164158068979425 25.46854571136652 26.6876404973159 24.679655722338158 58-59 22.45447437711812 25.81313853806501 27.267893476992654 24.46449360782421 60-61 23.306428876623286 25.928135900704653 26.724441860573712 24.040993362098355 62-63 23.31630113881079 25.830730810799924 26.489212850238804 24.36375520015048 64-65 22.696999077440825 25.413564407576455 26.59393954077541 25.29549697420731 66-67 22.623790450291953 25.307002973731468 26.507298777491904 25.561907798484672 68-69 22.005383422876978 25.526085335253562 26.94693549908354 25.52159574278592 70-71 22.21692428417614 25.218884245487892 27.1019800354034 25.46221143493257 72-73 22.248061987212637 25.795633581735217 26.37836768612532 25.577936744926827 74-75 22.4688873903067 25.693884908888148 26.153239272973554 25.68398842783159 76-77 22.077661327703094 25.32006376132403 26.35245216970158 26.249822741271302 78-79 21.851368052878424 25.239726769604204 26.751609507240893 26.157295670276476 80-81 21.900583948348302 25.54523941605019 26.361787934224623 26.192388701376885 82-83 22.465204119024833 25.301465532611672 26.4727268268727 25.7606035214908 84-85 22.50135609306795 25.614475679417044 25.846392960331276 26.037775267183733 86-87 21.635204938786007 25.73597347063928 26.26471824671695 26.364103343857764 88-89 21.748052949267173 25.059535803247858 26.512735040488728 26.679676206996238 90-91 22.462610948994026 24.72254038627683 26.399147566057756 26.41570109867139 92-93 22.289584931019373 24.977842825352127 26.213667269299002 26.518904974329498 94-95 21.655287168753873 24.716770022453506 26.465913604933295 27.162029203859326 96-97 21.483859373418717 24.44111790355543 26.775506135928385 27.299516587097468 98-99 21.29436414044147 24.790717802724846 26.566378087966026 27.34853996886765 100 21.20651931664783 24.904135208580445 26.46809632293412 27.4212491518376 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 272.0 1 200.0 2 334.0 3 540.5 4 404.0 5 294.5 6 438.5 7 795.5 8 1220.5 9 1492.0 10 1447.0 11 1349.5 12 1495.5 13 1731.0 14 2028.5 15 2351.0 16 2660.5 17 2982.0 18 3290.0 19 3554.0 20 3966.5 21 4523.0 22 5079.0 23 5945.5 24 7085.0 25 8754.5 26 11366.5 27 14632.5 28 18921.5 29 25555.5 30 34907.5 31 44510.0 32 56541.0 33 70270.5 34 84698.0 35 100569.5 36 118053.0 37 139512.5 38 154394.0 39 161450.5 40 167727.0 41 173755.0 42 183083.5 43 197331.0 44 214693.0 45 237901.5 46 262062.0 47 281705.0 48 303529.0 49 326035.0 50 344463.0 51 359335.0 52 363704.5 53 351036.5 54 328703.0 55 296447.5 56 263360.0 57 232148.5 58 198034.5 59 166607.5 60 130308.0 61 96583.5 62 74251.5 63 56984.0 64 41176.0 65 28333.5 66 19872.5 67 14657.0 68 10976.0 69 7834.5 70 5779.5 71 4315.5 72 3181.0 73 2368.5 74 1771.0 75 1222.0 76 762.5 77 478.5 78 308.0 79 187.5 80 117.5 81 82.5 82 56.0 83 38.0 84 27.0 85 21.0 86 15.0 87 7.0 88 5.0 89 5.5 90 3.5 91 1.0 92 1.0 93 1.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.051715520858034034 2 0.0 3 1.4592415591996059E-5 4 0.01611002681356365 5 0.0037064735603669994 6 0.0 7 2.3347864947193694E-4 8 3.794028053918976E-4 9 0.0019116064425514837 10-11 7.077321562118089E-4 12-13 2.40774857267935E-4 14-15 4.3777246775988185E-5 16-17 8.755449355197637E-5 18-19 0.0 20-21 2.1888623387994092E-5 22-23 0.0 24-25 8.755449355197637E-5 26-27 1.4592415591996059E-5 28-29 0.0010214690914397242 30-31 8.901373511117597E-4 32-33 0.013797128942232275 34-35 0.022275322401181984 36-37 0.02974663918428397 38-39 0.01988946245189063 40-41 1.386279481239626E-4 42-43 0.001721905039855535 44-45 0.010024989511701294 46-47 0.014183827955420171 48-49 0.005421082392426536 50-51 0.010696240628933111 52-53 0.016358097878627582 54-55 0.00974043740765737 56-57 0.003042518650931179 58-59 0.008062309614577823 60-61 0.0015613884683435783 62-63 9.9958046805173E-4 64-65 0.01870747678893895 66-67 0.04470386516607993 68-69 0.05400653010597742 70-71 0.0474253506739872 72-73 0.04016562391696916 74-75 0.028528172482352294 76-77 0.029381828794484068 78-79 0.01248381153895263 80-81 0.0018751254035714936 82-83 0.027499407183116577 84-85 0.04719916823231125 86-87 0.04752020137533517 88-89 0.059172245225544026 90-91 0.03463509840760265 92-93 0.026572788793024826 94-95 0.007194060886854058 96-97 0.0035678456122430367 98-99 0.02159677507615417 100 0.018940955438410886 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6852875.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.03905022965313 #Duplication Level Percentage of deduplicated Percentage of total 1 78.15342781516036 48.48564433844303 2 13.245391224982528 16.434629830361956 3 4.006618600420859 7.457004378077167 4 1.666009120757395 4.13430494102913 5 0.8311614932747852 2.5782234815113947 6 0.5001150731486135 1.8615998486204086 7 0.3178929101026897 1.380524195225796 8 0.23105891442224644 1.1467740478280695 9 0.17327798227822738 0.9675001301626703 >10 0.8067336973921881 8.794344585131027 >50 0.04391682593028564 1.8711660066995612 >100 0.021363627900538907 2.4972976133016496 >500 0.0019656557067760858 0.8523581472957396 >1k 9.547365728611763E-4 1.0551122733165583 >5k 1.1232194970667934E-4 0.4835161829958236 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7417 0.10823194644583478 No Hit GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC 7250 0.10579501304197143 No Hit GTACATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG 7170 0.10462761979461174 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.459241559199606E-5 2 0.0 0.0 0.0 0.0 1.459241559199606E-5 3 0.0 0.0 0.0 0.0 1.459241559199606E-5 4 0.0 0.0 0.0 0.0 1.459241559199606E-5 5 0.0 0.0 0.0 1.459241559199606E-5 1.459241559199606E-5 6 0.0 0.0 0.0 1.459241559199606E-5 2.918483118399212E-5 7 0.0 0.0 0.0 1.459241559199606E-5 2.918483118399212E-5 8 0.0 0.0 0.0 1.459241559199606E-5 7.29620779599803E-5 9 0.0 0.0 0.0 2.918483118399212E-5 1.0214690914397242E-4 10-11 0.0 0.0 0.0 3.648103897999015E-5 1.0944311693997046E-4 12-13 0.0 0.0 0.0 5.836966236798424E-5 1.3133174032796453E-4 14-15 0.0 7.29620779599803E-6 0.0 8.755449355197636E-5 2.2618244167593894E-4 16-17 0.0 1.459241559199606E-5 0.0 8.755449355197636E-5 3.064407274319173E-4 18-19 0.0 1.459241559199606E-5 0.0 1.1673932473596848E-4 3.137369352279153E-4 20-21 0.0 1.459241559199606E-5 0.0 3.502179742079054E-4 3.429217664119074E-4 22-23 0.0 1.459241559199606E-5 0.0 0.001422760520219616 3.8669901318789555E-4 24-25 0.0 1.459241559199606E-5 0.0 0.003648103897999015 4.085876365758897E-4 26-27 0.0 1.459241559199606E-5 0.0 0.006639549094358207 4.3047625996388376E-4 28-29 0.0 1.459241559199606E-5 0.0 0.01488426390383598 4.4506867555587986E-4 30-31 0.0 1.459241559199606E-5 0.0 0.05048246174051037 4.523648833518779E-4 32-33 0.0 1.459241559199606E-5 0.0 0.1392335333710304 5.691042080878463E-4 34-35 0.0 2.918483118399212E-5 0.0 0.2617441584736333 5.691042080878463E-4 36-37 0.0 5.107345457198622E-5 0.0 0.4491253670904547 5.764004158838444E-4 38-39 0.0 7.29620779599803E-5 0.0 0.7903690057092826 5.909928314758405E-4 40-41 0.0 7.29620779599803E-5 0.0 1.2302281888988198 6.128814548638345E-4 42-43 0.0 7.29620779599803E-5 0.0 1.6485854477135509 6.420662860478266E-4 44-45 0.0 7.29620779599803E-5 0.0 2.0999288619739893 6.639549094358207E-4 46-47 0.0 7.29620779599803E-5 0.0 2.592678255476716 6.858435328238148E-4 48-49 0.0 7.29620779599803E-5 0.0 3.1384491910329606 7.150283640078069E-4 50-51 0.0 7.29620779599803E-5 0.0 3.739138682669682 7.150283640078069E-4 52-53 0.0 7.29620779599803E-5 0.0 4.368276088503 7.661018185797931E-4 54-55 0.0 8.755449355197636E-5 0.0 5.036404428798132 8.317676887437754E-4 56-57 0.0 8.755449355197636E-5 0.0 5.700454188935301 8.536563121317695E-4 58-59 0.0 8.755449355197636E-5 0.0 6.4747496488700005 8.755449355197636E-4 60-61 0.0 1.0944311693997046E-4 0.0 7.261696733122959 8.901373511117596E-4 62-63 0.0 1.1673932473596848E-4 0.0 8.172993086843114 9.047297667037558E-4 64-65 0.0 1.1673932473596848E-4 0.0 8.938365284643307 9.339145978877479E-4 66-67 0.0 1.1673932473596848E-4 0.0 9.76782007551575 9.339145978877479E-4 68-69 0.0 1.1673932473596848E-4 0.0 10.69128650383963 9.339145978877479E-4 70-71 0.0 1.240355325319665E-4 0.0 11.64949747368805 9.412108056837459E-4 72-73 0.0 1.3133174032796453E-4 0.0 12.504445214599713 0.0010068766758477281 74-75 0.0 1.3133174032796453E-4 0.0 13.322525217518194 0.0010214690914397242 76-77 0.0 1.386279481239626E-4 0.0 14.204053043430676 0.0010214690914397242 78-79 0.0 1.459241559199606E-4 0.0 15.168035313645733 0.0010214690914397242 80-81 0.0 1.459241559199606E-4 0.0 16.068270616347153 0.0010433577148277183 82-83 0.0 1.459241559199606E-4 0.0 16.98484942451161 0.0010652463382157123 84-85 0.0 1.459241559199606E-4 0.0 17.884158108822938 0.0010871349616037064 86-87 0.0 1.459241559199606E-4 0.0 18.793302081243276 0.0011163197927876985 88 0.0 1.459241559199606E-4 0.0 19.472936541232695 0.0011236160005836965 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 8595 0.0 57.57016 2 ACCTGGG 9920 0.0 48.980568 3 GGTATCA 7365 0.0 43.402935 1 GTATCAA 12720 0.0 40.504787 1 CCTGGGG 9390 0.0 38.085194 4 TGGGGGG 18335 0.0 35.876347 6 CTGGGGG 6585 0.0 35.178776 5 GTACATG 26870 0.0 34.74518 1 TACATGG 27425 0.0 33.70332 2 GTACCTG 15175 0.0 33.022617 1 ATGGGGG 17945 0.0 32.259438 5 ACATGGG 27605 0.0 32.223877 3 CAACGCA 15600 0.0 32.07854 5 ATCAACG 15770 0.0 31.674994 3 CATGGGG 22945 0.0 31.561068 4 TATCAAC 16795 0.0 30.413364 2 TAACGCA 775 0.0 30.31834 4 ATAACGC 800 0.0 29.95678 3 TCAACGC 16825 0.0 29.941729 4 AACGCAG 17400 0.0 29.406254 6 >>END_MODULE