FastQCFastQC Report
Mon 6 Jun 2016
ERR1211423_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211423_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7219249
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC114490.15858990318799088No Hit
GTACATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG98550.1365100441888069No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG88600.057.579432
ACCTGGG104550.048.0639573
TGGGGGG238850.039.499726
GTACATG344500.039.219761
TACATGG350850.038.04142
ACATGGG353800.036.7103843
CCTGGGG107200.036.395374
ATGGGGG236500.036.0155375
CATGGGG294550.035.7639664
GTATCAA129600.035.5144041
GGTATCA84150.035.3256571
CTGGGGG77750.033.4944045
GTACTAG36850.032.7278481
GTACCTG162850.031.8503741
CAACGCA143450.030.2129575
ATCAACG150150.028.89633
GGGTTAG35250.028.6002371
TATCAAC160900.028.0208472
AACGCAG163300.027.4045476
TCAACGC163900.026.7587324
Produced by FastQC (version 0.11.5)