FastQCFastQC Report
Mon 6 Jun 2016
ERR1211423_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211423_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7219249
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC106910.1480901960854931No Hit
GTACATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG93720.12981959757864012No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG85800.056.088062
ACCTGGG102100.046.4903073
TGGGGGG211850.039.080226
GTACATG336350.036.3332561
CCTGGGG100450.035.885684
TACATGG339250.035.6571352
GGTATCA82700.034.9102021
CTGGGGG68900.034.7810365
GTATCAA126950.034.668311
ATGGGGG225200.034.5319565
ACATGGG337350.034.507393
CATGGGG281400.033.28584
GTACTAG33850.031.1156461
GTACCTG161900.030.0595841
CAACGCA144400.029.2864595
TAGTACT50950.028.7796964
GGGTTAG32400.028.7348981
ATCAACG148550.028.4099483
CTGTGCG55550.027.4054839
CTAGTAC55750.027.3128433
Produced by FastQC (version 0.11.5)