##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211423_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7219249 Sequences flagged as poor quality 0 Sequence length 100 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.07588697937971 33.0 31.0 34.0 30.0 34.0 2 32.19999670325819 33.0 31.0 34.0 30.0 34.0 3 32.2861686859672 34.0 31.0 34.0 30.0 34.0 4 35.93274591304442 37.0 35.0 37.0 35.0 37.0 5 35.78651740645045 37.0 35.0 37.0 35.0 37.0 6 35.8155251328774 37.0 35.0 37.0 35.0 37.0 7 35.735706719632475 37.0 35.0 37.0 33.0 37.0 8 35.70906489026767 37.0 35.0 37.0 33.0 37.0 9 37.29992759634693 39.0 37.0 39.0 34.0 39.0 10-11 37.263474912695216 39.0 37.0 39.0 34.0 39.0 12-13 37.14713954318517 39.0 37.0 39.0 33.0 39.0 14-15 38.359026749181254 40.0 38.0 41.0 33.0 41.0 16-17 38.31899523066734 40.0 38.0 41.0 33.0 41.0 18-19 38.28214146651543 40.0 38.0 41.0 33.5 41.0 20-21 38.22698849977331 40.0 38.0 41.0 33.5 41.0 22-23 38.13196331086516 40.0 37.0 41.0 33.0 41.0 24-25 38.0127425304211 40.0 37.0 41.0 33.0 41.0 26-27 37.900655802286366 40.0 37.0 41.0 33.0 41.0 28-29 37.817512874261574 40.0 37.0 41.0 33.0 41.0 30-31 37.93974913457065 40.0 37.0 41.0 33.0 41.0 32-33 37.82884009126157 40.0 37.0 41.0 33.0 41.0 34-35 37.75814638059998 40.0 37.0 41.0 33.0 41.0 36-37 37.665199247179316 40.0 37.0 41.0 32.0 41.0 38-39 37.57533858438738 40.0 37.0 41.0 32.0 41.0 40-41 37.535724076008464 40.0 36.0 41.0 32.0 41.0 42-43 37.44320988235757 40.0 36.0 41.0 32.0 41.0 44-45 37.34608530610316 39.0 36.0 41.0 31.5 41.0 46-47 37.19989842433749 39.0 36.0 41.0 31.0 41.0 48-49 37.04555951734038 39.0 35.0 41.0 31.0 41.0 50-51 36.881419175318655 39.0 35.0 40.5 31.0 41.0 52-53 36.66962581564925 39.0 35.0 40.0 31.0 41.0 54-55 36.44379006736019 38.0 35.0 40.0 30.0 41.0 56-57 36.19397447019766 38.0 35.0 40.0 30.0 41.0 58-59 35.901328656207866 38.0 34.0 40.0 29.0 41.0 60-61 35.607516862211014 37.0 34.0 40.0 29.0 41.0 62-63 35.25884209008444 36.5 34.0 39.5 28.5 41.0 64-65 34.89516153273007 36.0 34.0 39.0 28.0 40.5 66-67 34.52932992060532 35.5 33.0 39.0 28.0 40.0 68-69 34.14971072475821 35.0 33.0 38.0 27.5 40.0 70-71 33.77438047918835 35.0 33.0 37.0 26.5 39.5 72-73 33.41635466514592 35.0 32.5 37.0 26.0 39.0 74-75 33.0636230998543 35.0 32.0 36.0 26.0 39.0 76-77 32.07836916277579 34.0 30.5 35.0 25.5 37.0 78-79 32.58924286999935 34.5 32.0 35.0 26.0 37.0 80-81 32.52129480504135 34.5 32.0 35.0 26.0 37.0 82-83 32.2848263025697 34.0 32.0 35.0 26.0 36.0 84-85 32.04897102177803 34.0 31.5 35.0 25.0 36.0 86-87 31.846534036989166 34.0 31.0 35.0 25.0 35.5 88-89 31.678040472076802 34.0 31.0 35.0 25.0 35.0 90-91 31.48129916283536 34.0 31.0 35.0 24.0 35.0 92-93 31.306091326119933 34.0 31.0 35.0 24.0 35.0 94-95 31.148376098400263 34.0 30.5 35.0 24.0 35.0 96-97 30.9966945315226 34.0 30.0 35.0 23.0 35.0 98-99 30.812783227175018 34.0 30.0 35.0 23.0 35.0 100 28.908328830325704 32.0 26.0 34.0 18.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.20999489402868932 1101 2 -0.22115885405255398 1101 3 -0.2624414068895504 1101 4 -0.14283679326298682 1101 5 -0.12703046233801274 1101 6 -0.18931315656117675 1101 7 -0.2289607981815891 1101 8 -0.2680534623394948 1101 9 -0.34415147247219124 1101 10-11 -0.36490617601096176 1101 12-13 -0.4117446422718558 1101 14-15 -0.5351147373525791 1101 16-17 -0.5804468680199406 1101 18-19 -0.5324140502879686 1101 20-21 -0.6321452733400577 1101 22-23 -0.6078994638246868 1101 24-25 -0.6422055912962392 1101 26-27 -0.6611993693021105 1101 28-29 -0.7008424754753619 1101 30-31 -0.6945313788413827 1101 32-33 -0.6145784537530616 1101 34-35 -0.7328047897895615 1101 36-37 -0.6440487413512699 1101 38-39 -0.7156339259171389 1101 40-41 -0.7351990876689456 1101 42-43 -0.7328766053505902 1101 44-45 -0.7572864685924827 1101 46-47 -0.8715666895396055 1101 48-49 -0.8842472139890916 1101 50-51 -0.922932084820907 1101 52-53 -0.85365463475609 1101 54-55 -0.970054786764166 1101 56-57 -1.0496951589821961 1101 58-59 -1.0004676786845863 1101 60-61 -0.9804139314511886 1101 62-63 -0.9926285424413095 1101 64-65 -1.049533541436766 1101 66-67 -1.0534866897055934 1101 68-69 -1.0656831902574666 1101 70-71 -1.16477158025765 1101 72-73 -1.072330167612627 1101 74-75 -1.230764424066173 1101 76-77 -1.0776667470838852 1101 78-79 -1.0326083905946923 1101 80-81 -0.9699985730874694 1101 82-83 -0.9906893341314458 1101 84-85 -1.0827008965871059 1101 86-87 -1.2219191631308206 1101 88-89 -1.268558301413119 1101 90-91 -1.411245359399235 1101 92-93 -1.5300094241014293 1101 94-95 -1.6269645978382492 1101 96-97 -1.615253758278186 1101 98-99 -1.7577531001851199 1101 100 -1.6920022772894896 1102 1 -0.3320237961540471 1102 2 -0.2829663085898062 1102 3 -0.3130842027922611 1102 4 -0.16840716463612182 1102 5 -0.20099790514294114 1102 6 -0.32974037103706166 1102 7 -0.2611733562791514 1102 8 -0.3967039068140181 1102 9 -0.535347154075005 1102 10-11 -0.5315971534036805 1102 12-13 -0.525421484472119 1102 14-15 -0.6284018182604783 1102 16-17 -0.7510516466851556 1102 18-19 -0.7250362807829731 1102 20-21 -0.7587666464730418 1102 22-23 -0.7616324293948296 1102 24-25 -0.8127001538159817 1102 26-27 -0.8880677980733651 1102 28-29 -0.8609605319710099 1102 30-31 -0.8221158177204941 1102 32-33 -0.7978870815225676 1102 34-35 -0.8521201974877641 1102 36-37 -0.8726737469918078 1102 38-39 -0.9835464185844387 1102 40-41 -1.0307531578823799 1102 42-43 -1.013351313170638 1102 44-45 -1.0242356698920645 1102 46-47 -1.1155194669413504 1102 48-49 -1.1922751685037767 1102 50-51 -1.1152734164395213 1102 52-53 -1.211764602717821 1102 54-55 -1.2614449541753814 1102 56-57 -1.1729151738732142 1102 58-59 -1.238257376802899 1102 60-61 -1.245014017187387 1102 62-63 -1.2367822922720961 1102 64-65 -1.2384224679292615 1102 66-67 -1.2461143351187118 1102 68-69 -1.2338145922700008 1102 70-71 -1.2036187667564562 1102 72-73 -1.3768913900303907 1102 74-75 -1.3737434186963782 1102 76-77 -1.1955589902137085 1102 78-79 -1.1482961415987063 1102 80-81 -1.0852589968048107 1102 82-83 -1.1295061181213377 1102 84-85 -1.172011453421181 1102 86-87 -1.1968363036028222 1102 88-89 -1.2445803672043745 1102 90-91 -1.2423118162378195 1102 92-93 -1.3087427845093522 1102 94-95 -1.3567753577317525 1102 96-97 -1.502891105419561 1102 98-99 -1.5508658884166948 1102 100 -1.5945583442539757 1103 1 -0.34416997912406444 1103 2 -0.3311026218642752 1103 3 -0.4048744785591687 1103 4 -0.21522853810078146 1103 5 -0.2112128361162462 1103 6 -0.32266443827606395 1103 7 -0.34907346299693387 1103 8 -0.4088590622345336 1103 9 -0.4624235626477784 1103 10-11 -0.5060389922122468 1103 12-13 -0.6037237201076948 1103 14-15 -0.7436669133888429 1103 16-17 -0.8568035652261798 1103 18-19 -0.7686356608682132 1103 20-21 -0.7761019986077358 1103 22-23 -0.8745156108082668 1103 24-25 -0.8387964921045565 1103 26-27 -0.9610515457028015 1103 28-29 -0.9133151884688928 1103 30-31 -0.8409166021301786 1103 32-33 -0.8594713647801058 1103 34-35 -0.8765669983333524 1103 36-37 -0.8131870459307535 1103 38-39 -0.9012732618452404 1103 40-41 -0.9756771979321357 1103 42-43 -1.0126051383573937 1103 44-45 -0.9857334233807791 1103 46-47 -1.0678903690124883 1103 48-49 -1.1754194346320972 1103 50-51 -1.098899611916579 1103 52-53 -1.1434297060029621 1103 54-55 -1.1831486322003641 1103 56-57 -1.221459393779817 1103 58-59 -1.2458099370100868 1103 60-61 -1.1941670382522531 1103 62-63 -1.1656955381920113 1103 64-65 -1.1398743055822749 1103 66-67 -1.1060681134040138 1103 68-69 -1.103868053120891 1103 70-71 -1.0707237920084651 1103 72-73 -1.1081201059503698 1103 74-75 -1.1261957865396575 1103 76-77 -1.1226993274962815 1103 78-79 -0.9966022517015531 1103 80-81 -0.9495523143683826 1103 82-83 -0.954197860068227 1103 84-85 -0.9850574644192314 1103 86-87 -0.9909062275409113 1103 88-89 -0.955210172053576 1103 90-91 -1.1676435929869413 1103 92-93 -1.1013288214731283 1103 94-95 -1.1932547584808155 1103 96-97 -1.4057583811252918 1103 98-99 -1.5356535404215634 1103 100 -1.5870341288335972 1104 1 -0.3809349959735364 1104 2 -0.32672775505290375 1104 3 -0.37122382631509865 1104 4 -0.20021865348357437 1104 5 -0.2524183721230102 1104 6 -0.3560223381463459 1104 7 -0.32109720580817225 1104 8 -0.38013642350621524 1104 9 -0.47865706997923496 1104 10-11 -0.46694988231739387 1104 12-13 -0.5944694339343499 1104 14-15 -0.737292207614118 1104 16-17 -0.73912602826654 1104 18-19 -0.6935503173856432 1104 20-21 -0.6748606577525393 1104 22-23 -0.7727240005341045 1104 24-25 -0.8984128755138912 1104 26-27 -0.9084658434729818 1104 28-29 -0.9773209963836962 1104 30-31 -0.978165708550371 1104 32-33 -0.8169060716381509 1104 34-35 -0.8221522331641395 1104 36-37 -0.7759258686131503 1104 38-39 -0.9691773347934785 1104 40-41 -1.007611252559819 1104 42-43 -0.8694035677979954 1104 44-45 -0.9664512380432271 1104 46-47 -0.8982031235437802 1104 48-49 -0.9553221600059842 1104 50-51 -0.9618465491101915 1104 52-53 -0.8237824205482056 1104 54-55 -0.8078041914491934 1104 56-57 -0.9490636316650054 1104 58-59 -1.0408974105972888 1104 60-61 -1.0332449975243279 1104 62-63 -1.0768745821641517 1104 64-65 -1.024537657250768 1104 66-67 -1.0814243007870985 1104 68-69 -1.0962726475885276 1104 70-71 -1.0187040472538271 1104 72-73 -0.9730761191069703 1104 74-75 -0.9839598192661754 1104 76-77 -0.961175203376218 1104 78-79 -0.8342642325947338 1104 80-81 -0.9521509141442834 1104 82-83 -0.9744268093228641 1104 84-85 -0.9181012743324217 1104 86-87 -0.9122743214964295 1104 88-89 -0.9359938445823239 1104 90-91 -0.8946052257103183 1104 92-93 -1.105323726759746 1104 94-95 -1.1937328902438686 1104 96-97 -1.2990282757956386 1104 98-99 -1.2630680259003597 1104 100 -1.4247149034244089 1105 1 -0.33773565049866505 1105 2 -0.33822924608318417 1105 3 -0.31855219940852564 1105 4 -0.1984057687608498 1105 5 -0.20193815327238696 1105 6 -0.2719116466147966 1105 7 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-0.8809904002379341 1105 70-71 -0.910284457992212 1105 72-73 -0.9942443059802031 1105 74-75 -0.9024678745993313 1105 76-77 -0.7785697754524143 1105 78-79 -0.7239584675978108 1105 80-81 -0.6550880441283446 1105 82-83 -0.8118588911200675 1105 84-85 -0.665894458892005 1105 86-87 -0.7304014195025132 1105 88-89 -0.7078994558828171 1105 90-91 -0.7572398109368592 1105 92-93 -0.8804054401368333 1105 94-95 -0.8859654305359008 1105 96-97 -1.006964370371822 1105 98-99 -1.0280874911842304 1105 100 -0.9995910968695618 1106 1 -0.34517661189113014 1106 2 -0.2997787389977411 1106 3 -0.3432900265935892 1106 4 -0.1978288334959899 1106 5 -0.22409032340389956 1106 6 -0.2568250962116707 1106 7 -0.2909958754275124 1106 8 -0.40451778811104333 1106 9 -0.4962305093403856 1106 10-11 -0.4992401334623864 1106 12-13 -0.5786831168910496 1106 14-15 -0.7199767077567145 1106 16-17 -0.6452398236261416 1106 18-19 -0.6879377215495879 1106 20-21 -0.5977778486864906 1106 22-23 -0.6184350747368015 1106 24-25 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0.9237205016744028 2311 32-33 1.0614471770324698 2311 34-35 1.0167595001686038 2311 36-37 1.1287246445860575 2311 38-39 1.1434977333087346 2311 40-41 1.0715906848194763 2311 42-43 1.1465446385923812 2311 44-45 1.1162717384155911 2311 46-47 1.1670322631448187 2311 48-49 1.1830057826450329 2311 50-51 1.1559803373731086 2311 52-53 1.104345754799347 2311 54-55 1.1865015502377148 2311 56-57 1.2214626929419055 2311 58-59 1.2414801895503942 2311 60-61 1.260107084924158 2311 62-63 1.2439528669366098 2311 64-65 1.2574326695341185 2311 66-67 1.267284234185361 2311 68-69 1.2800846683367197 2311 70-71 1.2874743539327014 2311 72-73 1.3015322569635472 2311 74-75 1.2064737470411444 2311 76-77 1.1162907997818756 2311 78-79 1.0580890642631218 2311 80-81 1.1335790136026276 2311 82-83 1.131392941236598 2311 84-85 1.165198219066017 2311 86-87 1.2509240064771276 2311 88-89 1.2397051081217398 2311 90-91 1.3389599454208856 2311 92-93 1.3880281133477403 2311 94-95 1.4556752252363303 2311 96-97 1.4900233690728122 2311 98-99 1.5036796160251527 2311 100 1.6700224440662765 2312 1 0.1337788559695383 2312 2 0.16440677272095172 2312 3 0.32011917727726313 2312 4 0.24223539965528573 2312 5 0.3278022775254712 2312 6 0.4001260108921443 2312 7 0.4511477424062278 2312 8 0.33791644689924993 2312 9 0.43356331596618247 2312 10-11 0.2947204810931936 2312 12-13 0.4718446839039956 2312 14-15 0.8208878823120287 2312 16-17 0.9072279178143461 2312 18-19 0.9046365219056582 2312 20-21 0.9346257901220554 2312 22-23 0.49944455750909356 2312 24-25 0.486112756687497 2312 26-27 0.8265991730406483 2312 28-29 0.9343424525369315 2312 30-31 0.928654200902713 2312 32-33 0.7783370058063142 2312 34-35 0.9247792790123768 2312 36-37 1.0148487686936107 2312 38-39 1.0421746046930238 2312 40-41 1.0538705262737267 2312 42-43 0.9760008167069572 2312 44-45 0.8511197481929003 2312 46-47 1.0551698643347578 2312 48-49 1.173734041468542 2312 50-51 1.1770134715146199 2312 52-53 1.1940495497724655 2312 54-55 1.2093766533154238 2312 56-57 1.1742362680061618 2312 58-59 1.2201287266713692 2312 60-61 1.2235093559660797 2312 62-63 1.1994094409264378 2312 64-65 1.2520092973682324 2312 66-67 1.2422767794181695 2312 68-69 1.2640997497959532 2312 70-71 1.0531817731536464 2312 72-73 1.1797275021596079 2312 74-75 1.2729132786394004 2312 76-77 1.2184409068373334 2312 78-79 1.0704085845064597 2312 80-81 0.9931266843147455 2312 82-83 0.9871911912592068 2312 84-85 0.877378169235687 2312 86-87 1.1197223854433673 2312 88-89 0.7479365632799997 2312 90-91 1.064826911005067 2312 92-93 1.261985596979013 2312 94-95 1.3631687506620658 2312 96-97 1.3744654468637698 2312 98-99 1.4516671663385097 2312 100 1.6552207862320714 2313 1 -0.4032641890167028 2313 2 -0.09718626894367333 2313 3 0.21063170319717983 2313 4 0.0694067182585556 2313 5 0.22351056886149934 2313 6 0.3226397422264853 2313 7 0.15236991211774153 2313 8 0.12775428070104056 2313 9 0.3036609053448842 2313 10-11 0.42718914545274345 2313 12-13 0.5969551122839647 2313 14-15 0.6523758532579294 2313 16-17 0.7756590952719691 2313 18-19 0.677553806472261 2313 20-21 0.8669998375342018 2313 22-23 0.9060801322359637 2313 24-25 0.7742434129433775 2313 26-27 0.9619570077942257 2313 28-29 0.8713416212302079 2313 30-31 0.8489835255620122 2313 32-33 1.0122085475840876 2313 34-35 1.0068571000611044 2313 36-37 0.873090513786849 2313 38-39 0.6079541525776975 2313 40-41 0.776798397015142 2313 42-43 0.8107623690711776 2313 44-45 1.0038203243258152 2313 46-47 1.0372568654494927 2313 48-49 1.0617599524523982 2313 50-51 0.999493907982739 2313 52-53 0.9508134860235984 2313 54-55 0.9832578922925137 2313 56-57 1.1066373862350574 2313 58-59 0.9355851537329727 2313 60-61 0.9382419659576016 2313 62-63 1.0097987930700327 2313 64-65 1.0758009354011762 2313 66-67 1.1084915413848364 2313 68-69 1.0570842625077503 2313 70-71 1.0050229054574729 2313 72-73 1.104436214812786 2313 74-75 0.6998861317313327 2313 76-77 0.8828066513023565 2313 78-79 0.7319633186930901 2313 80-81 0.8062357005354883 2313 82-83 0.8735740915542038 2313 84-85 0.9196542249035851 2313 86-87 1.0599850764165808 2313 88-89 1.018292450711911 2313 90-91 1.1320747132461229 2313 92-93 0.8692424859524586 2313 94-95 0.8880671801572113 2313 96-97 1.196216748416571 2313 98-99 1.3247168245536827 2313 100 1.3618404060666407 2314 1 -0.3128817839754028 2314 2 -0.16734760260182213 2314 3 0.1300275020064774 2314 4 -0.1452598472004567 2314 5 0.21210025202594096 2314 6 0.18927538912807762 2314 7 0.10907720375133323 2314 8 0.1401086544207928 2314 9 0.19545467336792655 2314 10-11 0.42808003110314274 2314 12-13 0.5476713795089054 2314 14-15 0.6062087682014621 2314 16-17 0.7888694986089675 2314 18-19 0.8574674315607069 2314 20-21 0.5193598581306986 2314 22-23 0.7064115695827411 2314 24-25 0.8984026698066359 2314 26-27 0.884232406714375 2314 28-29 0.8578946221844674 2314 30-31 0.8358980988807687 2314 32-33 0.9317844347904085 2314 34-35 0.9480485724452095 2314 36-37 0.8809159956032246 2314 38-39 0.874007309643126 2314 40-41 0.9282302569393224 2314 42-43 0.7227828837971586 2314 44-45 0.737010927575227 2314 46-47 0.9062757944485895 2314 48-49 0.85739349431541 2314 50-51 0.9755723686010214 2314 52-53 1.0673936855824664 2314 54-55 1.1342719516545756 2314 56-57 0.9375107165396983 2314 58-59 0.9569989527161482 2314 60-61 1.020617857114054 2314 62-63 0.7414074374779034 2314 64-65 0.8642227208086695 2314 66-67 0.9614213109239174 2314 68-69 1.0587375217076769 2314 70-71 1.0922105374177917 2314 72-73 1.1121915393094284 2314 74-75 1.1209070390910938 2314 76-77 0.9173530605388258 2314 78-79 0.9096698690661142 2314 80-81 0.9381399280918359 2314 82-83 0.9423292085627537 2314 84-85 0.8210339270439313 2314 86-87 0.9549732985330763 2314 88-89 1.0524091106450584 2314 90-91 1.1486587552756014 2314 92-93 1.2337573243285895 2314 94-95 1.309814255981916 2314 96-97 1.3954870152659815 2314 98-99 1.3805511988385746 2314 100 1.3996110282748084 2315 1 -0.02710220202091307 2315 2 0.14844224862616073 2315 3 0.167543436769499 2315 4 -0.006257293861551716 2315 5 0.27368575714337595 2315 6 0.41904685595927305 2315 7 0.45636212523496056 2315 8 0.3991482982122889 2315 9 0.6169932431267569 2315 10-11 0.6162661202845143 2315 12-13 0.6749083049774924 2315 14-15 0.7867401846142315 2315 16-17 0.7939091247753112 2315 18-19 0.805083547212341 2315 20-21 0.8738867169730611 2315 22-23 0.9190885017111086 2315 24-25 0.85381806577508 2315 26-27 0.9880951881437241 2315 28-29 0.9909770130530475 2315 30-31 0.9602270120604217 2315 32-33 0.970789911229204 2315 34-35 1.0065209040523584 2315 36-37 1.040966868032477 2315 38-39 1.0197214655667537 2315 40-41 1.016404889772268 2315 42-43 1.0747927228281213 2315 44-45 0.9625718388396152 2315 46-47 1.0013613947208597 2315 48-49 1.123349901912981 2315 50-51 1.0068285026520485 2315 52-53 1.065371927280573 2315 54-55 0.9744359382141425 2315 56-57 1.0468023396724107 2315 58-59 1.0853574358116873 2315 60-61 1.1493096575264232 2315 62-63 1.1617752395050687 2315 64-65 1.146730502890037 2315 66-67 1.1873960985119965 2315 68-69 1.0587050646765874 2315 70-71 0.7101033524723519 2315 72-73 0.9381025157186897 2315 74-75 1.1533874523547354 2315 76-77 1.075688103494361 2315 78-79 0.9670373436641242 2315 80-81 0.9482311141207944 2315 82-83 0.9873288232748436 2315 84-85 0.92101522130287 2315 86-87 0.9526019157150927 2315 88-89 1.045527485673233 2315 90-91 1.1256236068943828 2315 92-93 1.203307068853075 2315 94-95 1.268421023956133 2315 96-97 1.3423586649791126 2315 98-99 1.2522025839918811 2315 100 1.128834122091078 2316 1 0.4888762300355012 2316 2 0.4950368554483262 2316 3 0.5007271856157729 2316 4 0.3077757193228834 2316 5 0.426225683336213 2316 6 0.4288698369249886 2316 7 0.48834426630603645 2316 8 0.525454537824892 2316 9 0.5328299894756583 2316 10-11 0.5004647305830119 2316 12-13 0.6444601941288965 2316 14-15 0.803958839501739 2316 16-17 0.8032345603655813 2316 18-19 0.8920038743954208 2316 20-21 0.9352600072478978 2316 22-23 0.9714197602004688 2316 24-25 1.0012189311254573 2316 26-27 1.0422205211750963 2316 28-29 1.0247379730954975 2316 30-31 0.9388258562387932 2316 32-33 0.8946853496127645 2316 34-35 0.7681295187921293 2316 36-37 0.7379053827060744 2316 38-39 0.7277081033711355 2316 40-41 0.8520518918032352 2316 42-43 1.0280232761538244 2316 44-45 0.9639539935403008 2316 46-47 1.0150516142611536 2316 48-49 0.7650775152861726 2316 50-51 0.8295557659568473 2316 52-53 1.0116256449756875 2316 54-55 1.0799223531770608 2316 56-57 1.129704803003328 2316 58-59 1.080647772317839 2316 60-61 1.1283231487627958 2316 62-63 1.1491804930183207 2316 64-65 1.1619376703004534 2316 66-67 1.1205685562926107 2316 68-69 1.0666982970333763 2316 70-71 0.9672361836005123 2316 72-73 0.9720977572762379 2316 74-75 1.037945977517424 2316 76-77 0.917791036295224 2316 78-79 0.8716394278536299 2316 80-81 0.870680473399787 2316 82-83 0.8015225349480559 2316 84-85 0.9096523281366906 2316 86-87 0.8870389404603429 2316 88-89 0.845227427060756 2316 90-91 0.943723107418581 2316 92-93 1.010647233227143 2316 94-95 0.8711947393399129 2316 96-97 1.0794173222919632 2316 98-99 1.1783711866152942 2316 100 0.8934746503186126 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 0.0 11 4.0 12 36.0 13 135.0 14 524.0 15 1310.0 16 2739.0 17 4732.0 18 7107.0 19 10284.0 20 14963.0 21 21123.0 22 27930.0 23 36710.0 24 46899.0 25 60388.0 26 77578.0 27 98952.0 28 122846.0 29 150917.0 30 187032.0 31 232402.0 32 294626.0 33 387688.0 34 558434.0 35 737329.0 36 1130656.0 37 1683830.0 38 1209460.0 39 112608.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.63729415557184 17.278819190073843 11.504988828251095 25.578897826103226 2 17.004441874771185 19.200819919080224 39.481184261687055 24.313553944461535 3 18.924436450998776 25.842345352313707 29.20347105404884 26.029747142638683 4 13.005306227117494 15.439955789060397 35.84073033945251 35.714007644369595 5 13.46132721165811 38.14796052439285 32.70048410161105 15.690228162337982 6 32.55581467765064 34.42612028288819 18.989623295944398 14.028441743516776 7 30.375759775267202 31.382971060665664 21.024623090519224 17.21664607354791 8 28.800645830917908 34.696346298571854 18.83694750144302 17.666060369067218 9 30.149805003879976 14.314089338891536 17.94039105608192 37.59571460114657 10-11 27.344870873626707 24.115643207770958 27.713160241370655 20.826325677231683 12-13 27.066333454791337 21.85053517822987 26.8057308878003 24.277400479178493 14-15 24.935105771065473 23.59769491210822 24.163252471950848 27.303946844875455 16-17 24.66397261595593 25.649055465045112 25.14412007745136 24.5428518415476 18-19 24.29350338241554 26.035852205679568 25.937829544319634 23.732814867585258 20-21 25.38709358826659 25.874450375655417 24.9123627679278 23.826093268150192 22-23 25.169893041491303 25.296180433197474 25.07437062696835 24.459555898342874 24-25 24.602146596554043 24.649055953519774 25.456917776132176 25.29187967379401 26-27 24.5708087996706 25.016589332890998 25.12182883326829 25.290773034170112 28-29 24.308287857304727 26.536814112612113 25.006323425847892 24.148574604235268 30-31 26.18047177037867 25.109735302714014 25.417934699045187 23.29185822786213 32-33 25.797279962284463 24.96348316221719 25.07547020948512 24.16376666601323 34-35 25.492134964095104 25.556373693585627 25.377983580018228 23.573507762301045 36-37 24.932724956231905 25.467066370237557 25.11349914057266 24.486709532957878 38-39 24.978121779443846 25.690950331157254 25.19413651912645 24.136791370272455 40-41 24.909576669281208 24.647866204706524 25.8846935012181 24.55786362479417 42-43 24.6504541102779 25.124591077038303 25.084004393058347 25.140950419625447 44-45 23.619670231678434 25.360822200146483 26.075470767600788 24.944036800574295 46-47 23.61156799755176 24.589361550178115 25.636188537055737 26.162881915214392 48-49 24.15871018898285 24.885141080525777 25.954798100973342 25.001350629518026 50-51 23.943453197305374 24.78834984919338 26.31989040406643 24.948306549434815 52-53 23.79521825799113 25.12037201809579 25.721780122548132 25.36262960136495 54-55 23.227667294978925 24.506110615512586 26.70847980503549 25.557742284473 56-57 24.14796244601781 24.57827541803296 25.688705768406027 25.585056367543203 58-59 23.288644928255987 25.05862937548858 26.44126998243855 25.21145571381689 60-61 24.384816586214885 25.065688339761277 25.895573945743443 24.65392112828039 62-63 24.17277567593306 25.00759261343468 25.70920896569039 25.110422744941864 64-65 23.48718059553426 24.532192891691366 25.849618008991666 26.13100850378271 66-67 23.454120847750193 24.65066944859874 25.677728708408214 26.217480995242852 68-69 22.728412343129577 24.831428828816343 26.226572515539786 26.21358631251429 70-71 22.926668713097552 24.52759667480389 26.369592346810443 26.17614226528812 72-73 22.94211783212764 25.196554206751614 25.555764108484386 26.30556385263636 74-75 23.295080361308244 25.15532649219221 25.382810299316205 26.166782847183338 76-77 22.971744111901785 24.65492090684652 25.45657684310755 26.916758138144147 78-79 22.883052519977827 24.841927762986057 25.7808091763564 26.494210540679713 80-81 23.0299292329909 25.39539313177347 25.08107471659432 26.49360291864131 82-83 23.550272623179232 25.15487812042418 25.313739731600016 25.98110952479657 84-85 23.511729696765517 25.529163120942826 24.64472141645376 26.314385765837894 86-87 22.64383621064191 25.741429016679856 25.11325448538207 26.501480287296165 88-89 22.755954584127313 24.95233345851294 25.367269550038795 26.924442407320946 90-91 23.693241322431774 24.687452628066687 25.009824263464296 26.609481786037243 92-93 23.478017616997914 24.96130014605666 24.95284835428027 26.60783388266516 94-95 22.994010582467947 24.75179851530712 25.015141163295713 27.23904973892922 96-97 22.616344924848875 24.64247407565632 25.32829052053979 27.41289047895501 98-99 22.66161839904429 24.78590872454117 25.091402577636856 27.461070298777685 100 22.411712311695812 25.115694863983464 25.116567692942652 27.356025131378075 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 18.0 1 15.5 2 18.0 3 37.5 4 47.0 5 43.5 6 62.0 7 109.0 8 181.0 9 249.0 10 282.5 11 299.5 12 352.0 13 430.0 14 503.5 15 586.5 16 700.5 17 802.0 18 921.0 19 1010.5 20 1131.0 21 1290.0 22 1441.0 23 1679.5 24 1995.5 25 2420.5 26 3188.5 27 4214.5 28 5686.0 29 8490.0 30 12833.0 31 18067.5 32 25820.5 33 36066.5 34 48838.0 35 63310.5 36 82426.0 37 106135.0 38 124546.5 39 139394.5 40 151145.0 41 160639.0 42 174999.5 43 194197.0 44 217289.5 45 249684.5 46 282487.0 47 311197.5 48 343722.0 49 373006.0 50 398710.0 51 424270.0 52 431444.0 53 418088.0 54 396531.0 55 359179.0 56 320285.0 57 282104.5 58 238446.0 59 198069.5 60 154207.5 61 114970.0 62 88725.5 63 68866.0 64 49861.5 65 33521.5 66 23329.5 67 17243.0 68 12885.0 69 9517.0 70 7203.5 71 5302.5 72 3859.0 73 2850.0 74 2060.5 75 1356.5 76 876.0 77 534.5 78 317.5 79 203.5 80 139.0 81 95.5 82 64.5 83 35.5 84 25.5 85 21.5 86 14.0 87 10.0 88 9.0 89 9.0 90 4.0 91 0.5 92 0.0 93 1.0 94 1.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.051625868563336716 2 0.0 3 1.3851856335749052E-5 4 0.015597190234053433 5 0.004294075464082206 6 1.3851856335749052E-5 7 1.2466670702174145E-4 8 3.047408393864791E-4 9 0.0018145931799831256 10-11 8.518891646485667E-4 12-13 2.5625934221135745E-4 14-15 2.0777784503623577E-5 16-17 1.1774077885386693E-4 18-19 0.0 20-21 0.0 22-23 1.3851856335749052E-5 24-25 9.696299435024336E-5 26-27 2.0777784503623577E-5 28-29 8.449632364806921E-4 30-31 8.17259523809194E-4 32-33 0.013900337832924173 34-35 0.02272397031879632 36-37 0.030945047054063382 38-39 0.020147525040346993 40-41 1.592963478611141E-4 42-43 0.0018353709644867492 44-45 0.009225336319608868 46-47 0.013955745258267168 48-49 0.005443779539949377 50-51 0.010153410694104054 52-53 0.015313227179170576 54-55 0.009758632788535206 56-57 0.002784223123485559 58-59 0.008283410088777933 60-61 0.0016830005447935096 62-63 8.518891646485667E-4 64-65 0.01860996898707885 66-67 0.04378571787730275 68-69 0.05400838785308555 70-71 0.04756727465696224 72-73 0.03955397576673141 74-75 0.028146972074242074 76-77 0.028576379620650294 78-79 0.012078818724773172 80-81 0.0018907783898297454 82-83 0.02716349027440389 84-85 0.0470616819007074 86-87 0.047373348668261754 88-89 0.05932057475784531 90-91 0.03356304790151995 92-93 0.025549749011289122 94-95 0.00716140972558226 96-97 0.003629186359966251 98-99 0.022045229358344614 100 0.01856148748990373 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7219249.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.2340993676174 #Duplication Level Percentage of deduplicated Percentage of total 1 75.96668795584232 43.47884967093463 2 13.9940525718138 16.01873990901706 3 4.5022267499663755 7.730426795493621 4 1.9364247007043287 4.433180949520814 5 1.008032523127068 2.8846916797222346 6 0.6083871291878976 2.089229364355177 7 0.4051679810959185 1.6232597143434508 8 0.2740089594220902 1.2546124808945083 9 0.19866212256543264 1.0233222897142584 >10 1.0076562911180036 10.180983183454538 >50 0.06020131651114666 2.3886107975819297 >100 0.03389184815394476 3.6792704326136203 >500 0.002874918500867074 1.1278643352974598 >1k 0.0016341460965667214 1.6294289720596216 >5k 6.052392947797904E-5 0.27235956269234635 >10k+ 3.026196473898952E-5 0.1851698623048569 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC 10691 0.1480901960854931 No Hit GTACATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG 9372 0.12981959757864012 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 1.385185633574905E-5 7 0.0 0.0 0.0 0.0 1.385185633574905E-5 8 0.0 0.0 0.0 0.0 1.385185633574905E-5 9 0.0 0.0 0.0 4.1555569007247154E-5 4.1555569007247154E-5 10-11 0.0 1.385185633574905E-5 0.0 4.1555569007247154E-5 4.1555569007247154E-5 12-13 0.0 1.385185633574905E-5 0.0 8.311113801449431E-5 5.54074253429962E-5 14-15 0.0 2.77037126714981E-5 0.0 1.2466670702174145E-4 9.696299435024336E-5 16-17 0.0 4.1555569007247154E-5 0.0 1.592963478611141E-4 1.5237041969323956E-4 18-19 0.0 4.1555569007247154E-5 0.0 2.216297013719848E-4 1.6622227602898862E-4 20-21 0.0 4.1555569007247154E-5 0.0 3.9477790556884795E-4 2.0085191686836123E-4 22-23 0.0 4.1555569007247154E-5 0.0 0.0011427781476992967 2.493334140434829E-4 24-25 0.0 5.54074253429962E-5 0.0 0.0028604083333321787 2.9781491121860456E-4 26-27 0.0 6.925928167874525E-5 0.0 0.005215223910409518 3.047408393864791E-4 28-29 0.0 6.925928167874525E-5 0.0 0.01155244818401471 3.047408393864791E-4 30-31 0.0 6.925928167874525E-5 0.0 0.03831423462468188 3.116667675543537E-4 32-33 0.0 6.925928167874525E-5 0.0 0.10440144120254059 3.185926957222282E-4 34-35 0.0 6.925928167874525E-5 0.0 0.1950964705608575 3.6014826472947534E-4 36-37 0.0 7.618520984661978E-5 0.0 0.34466881527427573 3.6014826472947534E-4 38-39 0.0 8.311113801449431E-5 0.0 0.6421512819408224 3.670741928973499E-4 40-41 0.0 8.311113801449431E-5 0.0 1.0397480402739951 3.740001210652244E-4 42-43 0.0 8.311113801449431E-5 0.0 1.4059841958630321 3.740001210652244E-4 44-45 0.0 9.696299435024335E-5 0.0 1.8116912160807863 3.740001210652244E-4 46-47 0.0 9.696299435024335E-5 0.0 2.2584412866213643 4.0170383373672246E-4 48-49 0.0 1.0388892251811788E-4 0.0 2.764941339466197 4.432594027439696E-4 50-51 0.0 1.108148506859924E-4 0.0 3.335215338880817 4.432594027439696E-4 52-53 0.0 1.108148506859924E-4 0.0 3.9319671616812224 5.055927562548403E-4 54-55 0.0 1.108148506859924E-4 0.0 4.5916687455994385 5.748520379335856E-4 56-57 0.0 1.108148506859924E-4 0.0 5.252651626228712 5.887038942693347E-4 58-59 0.0 1.108148506859924E-4 0.0 6.050809440150907 5.956298224372092E-4 60-61 0.0 1.1774077885386692E-4 0.0 6.84506795651459 5.956298224372092E-4 62-63 0.0 1.2466670702174145E-4 0.0 7.72868479810019 6.510372477802054E-4 64-65 0.0 1.2466670702174145E-4 0.0 8.502241715170097 6.510372477802054E-4 66-67 0.0 1.385185633574905E-4 0.0 9.339004652700025 6.579631759480799E-4 68-69 0.0 1.385185633574905E-4 0.0 10.305746484156455 6.648891041159545E-4 70-71 0.0 1.385185633574905E-4 0.0 11.366265383005906 6.648891041159545E-4 72-73 0.0 1.385185633574905E-4 0.0 12.300337611294472 7.549261702983234E-4 74-75 0.0 1.385185633574905E-4 0.0 13.199274605987409 7.757039548019468E-4 76-77 6.925928167874525E-6 1.385185633574905E-4 0.0 14.183289702294518 7.757039548019468E-4 78-79 1.385185633574905E-5 1.385185633574905E-4 0.0 15.229416522411126 7.757039548019468E-4 80-81 1.385185633574905E-5 1.385185633574905E-4 0.0 16.197273428302584 7.826298829698214E-4 82-83 1.385185633574905E-5 1.4544449152536503E-4 0.0 17.204642754391767 7.964817393055705E-4 84-85 1.385185633574905E-5 1.5237041969323956E-4 0.0 18.206055782256577 8.034076674734449E-4 86-87 1.385185633574905E-5 1.5237041969323956E-4 0.0 19.212787922954313 8.034076674734449E-4 88 1.385185633574905E-5 1.5237041969323956E-4 0.0 19.980554764075876 8.034076674734449E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 8580 0.0 56.08806 2 ACCTGGG 10210 0.0 46.490307 3 TGGGGGG 21185 0.0 39.08022 6 GTACATG 33635 0.0 36.333256 1 CCTGGGG 10045 0.0 35.88568 4 TACATGG 33925 0.0 35.657135 2 GGTATCA 8270 0.0 34.910202 1 CTGGGGG 6890 0.0 34.781036 5 GTATCAA 12695 0.0 34.66831 1 ATGGGGG 22520 0.0 34.531956 5 ACATGGG 33735 0.0 34.50739 3 CATGGGG 28140 0.0 33.2858 4 GTACTAG 3385 0.0 31.115646 1 GTACCTG 16190 0.0 30.059584 1 CAACGCA 14440 0.0 29.286459 5 TAGTACT 5095 0.0 28.779696 4 GGGTTAG 3240 0.0 28.734898 1 ATCAACG 14855 0.0 28.409948 3 CTGTGCG 5555 0.0 27.405483 9 CTAGTAC 5575 0.0 27.312843 3 >>END_MODULE