FastQCFastQC Report
Mon 6 Jun 2016
ERR1211422_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211422_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3200276
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC42170.1317698848474319No Hit
GTACATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG39350.12295814486000584No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32140.10042883801272141No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG63950.070.359062
ACCTGGG69100.063.0639573
CCTGGGG59550.050.284864
GTACCTG99050.046.3257141
CTGGGGG38950.044.0519335
TAACGCA5600.042.8116264
TGGGGGG98450.042.401076
TATAACG5700.042.1112372
GTACGTT4200.038.1370621
ATGGGGG84400.037.930125
CATGGGG108750.037.65034
ATAACGC6650.036.0518953
GTACATG166700.035.099721
TACATGG171400.033.802592
GTACCCG7650.033.2544631
ACATGGG167350.033.230633
GGGTTAG15200.031.6136191
TACGTTG5000.031.0632322
GTATCAA86850.030.0509051
TATCACG2550.027.6855932
Produced by FastQC (version 0.11.5)