##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211422_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3200276 Sequences flagged as poor quality 0 Sequence length 100 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.237365777201717 31.0 31.0 34.0 28.0 34.0 2 31.273828257312807 31.0 31.0 34.0 28.0 34.0 3 31.34903614563244 31.0 31.0 34.0 28.0 34.0 4 34.98470881886437 37.0 35.0 37.0 32.0 37.0 5 35.039549401364134 37.0 35.0 37.0 32.0 37.0 6 35.09544708018933 37.0 35.0 37.0 32.0 37.0 7 35.0205916614692 37.0 35.0 37.0 32.0 37.0 8 35.01159806216714 37.0 35.0 37.0 32.0 37.0 9 36.56753854980008 39.0 37.0 39.0 32.0 39.0 10-11 36.45927741857265 39.0 37.0 39.0 32.0 39.0 12-13 36.339350262289884 39.0 35.0 39.0 32.0 39.0 14-15 37.51193459564112 40.0 37.0 41.0 32.0 41.0 16-17 37.475347595019926 40.0 37.0 41.0 32.0 41.0 18-19 37.44549048269587 40.0 37.0 41.0 31.0 41.0 20-21 37.428615219437326 40.0 37.0 41.0 31.0 41.0 22-23 37.31687485704358 40.0 36.5 41.0 31.0 41.0 24-25 37.2258269286774 40.0 36.0 41.0 31.0 41.0 26-27 37.10330468372103 40.0 36.0 41.0 30.5 41.0 28-29 36.95203351210958 39.0 36.0 41.0 30.0 41.0 30-31 36.81861830042159 39.0 36.0 41.0 30.0 41.0 32-33 36.63449808703999 39.0 35.5 41.0 30.0 41.0 34-35 36.543560305423654 39.0 35.0 41.0 30.0 41.0 36-37 36.46238668164871 39.0 35.0 40.5 29.5 41.0 38-39 36.33115050076931 39.0 35.0 40.0 29.0 41.0 40-41 36.18050271289101 39.0 35.0 40.0 29.0 41.0 42-43 36.01550991227006 38.0 35.0 40.0 28.0 41.0 44-45 35.94233591102767 38.0 35.0 40.0 28.0 41.0 46-47 35.76981922809158 38.0 34.5 40.0 27.0 41.0 48-49 35.58351529680565 38.0 34.0 40.0 27.0 41.0 50-51 34.95615190689803 37.5 33.5 39.5 26.5 40.5 52-53 35.31113488336631 38.0 34.0 39.5 27.0 40.5 54-55 35.74134543395632 38.0 34.0 40.0 28.0 41.0 56-57 35.66971145613691 38.0 34.0 40.0 27.5 41.0 58-59 35.47968784567331 38.0 34.0 40.0 27.0 41.0 60-61 35.24000070618909 38.0 34.0 40.0 26.5 41.0 62-63 34.92287337092176 37.0 34.0 40.0 26.0 41.0 64-65 34.725531954118956 37.0 33.0 40.0 26.0 41.0 66-67 34.53279326533087 36.0 33.0 39.0 26.0 41.0 68-69 34.19230263264794 36.0 33.0 39.0 26.0 41.0 70-71 33.8139312046836 35.0 33.0 39.0 26.0 40.5 72-73 33.420860888248384 35.0 32.0 37.5 25.0 40.0 74-75 33.05123823695206 35.0 32.0 37.0 25.0 39.0 76-77 32.68172479498644 35.0 32.0 36.5 24.0 39.0 78-79 32.313114243896464 35.0 31.0 36.0 24.0 38.5 80-81 31.972608300034125 35.0 31.0 36.0 23.0 37.0 82-83 31.649818640642245 34.5 31.0 35.0 22.5 37.0 84-85 31.3417911455137 34.0 31.0 35.0 20.5 36.5 86-87 31.043040975215888 34.0 30.0 35.0 20.0 36.0 88-89 30.791006931902125 34.0 30.0 35.0 20.0 36.0 90-91 30.53537960475909 34.0 29.5 35.0 19.0 35.5 92-93 30.28018239676828 34.0 29.0 35.0 18.0 35.0 94-95 30.040663524021053 34.0 29.0 35.0 17.5 35.0 96-97 29.77039949054394 34.0 29.0 35.0 17.0 35.0 98-99 29.489996800275975 34.0 28.0 35.0 15.0 35.0 100 28.21787120860826 32.0 25.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.2794321946597158 1101 2 -0.2341529387539154 1101 3 -0.31608253064840497 1101 4 -0.2457941664274088 1101 5 -0.2098388491459673 1101 6 -0.19047219398856896 1101 7 -0.28149323326405096 1101 8 -0.16449229491431083 1101 9 -0.3047559902138204 1101 10-11 -0.2681540088537986 1101 12-13 -0.3757810249840716 1101 14-15 -0.5907460501618189 1101 16-17 -0.5676364321125291 1101 18-19 -0.5294602200440508 1101 20-21 -0.6215155082694466 1101 22-23 -0.5260681571339987 1101 24-25 -0.6059587654875358 1101 26-27 -0.6719710994864414 1101 28-29 -0.7468767654581754 1101 30-31 -0.7149711516531099 1101 32-33 -0.8597203760961847 1101 34-35 -0.8746320687979434 1101 36-37 -0.7320849102761855 1101 38-39 -0.7574169141471856 1101 40-41 -0.8962747975709391 1101 42-43 -0.9663599826697649 1101 44-45 -0.9520359986148108 1101 46-47 -0.9882668880508874 1101 48-49 -1.048577398993622 1101 50-51 -1.0122313850666274 1101 52-53 -0.761146501217226 1101 54-55 -0.7625781891366188 1101 56-57 -0.7914740688460782 1101 58-59 -0.7315601727909069 1101 60-61 -0.7796762072733046 1101 62-63 -0.9452775632629198 1101 64-65 -0.9584898293047388 1101 66-67 -0.9398290081291449 1101 68-69 -0.8728816907915444 1101 70-71 -0.8672831344555476 1101 72-73 -0.9193881567906175 1101 74-75 -1.0779874250939585 1101 76-77 -1.0039425395457755 1101 78-79 -1.198082179155712 1101 80-81 -1.358230659077595 1101 82-83 -1.2601932899025599 1101 84-85 -1.3202196607756669 1101 86-87 -1.3105647861976735 1101 88-89 -1.337845271248728 1101 90-91 -1.448555314598746 1101 92-93 -1.643356616920613 1101 94-95 -1.781339550236499 1101 96-97 -1.9833886196201966 1101 98-99 -2.0307082263740384 1101 100 -2.0061384605233883 1102 1 -0.46082851163493643 1102 2 -0.46632145019806615 1102 3 -0.5016173528640984 1102 4 -0.463037121989494 1102 5 -0.41333984499929954 1102 6 -0.32472465097097114 1102 7 -0.44476012195703163 1102 8 -0.4893363867287235 1102 9 -0.6910491302536528 1102 10-11 -0.5363834306058521 1102 12-13 -0.5119047883301349 1102 14-15 -0.7467032121430819 1102 16-17 -0.8221810332814314 1102 18-19 -0.8256694471965673 1102 20-21 -0.8729317423113017 1102 22-23 -1.004938924757333 1102 24-25 -0.8840385643578159 1102 26-27 -0.8825920385271644 1102 28-29 -0.9586731379399751 1102 30-31 -0.9812149887902777 1102 32-33 -1.006559766176835 1102 34-35 -1.2204931554208613 1102 36-37 -1.1096684150268956 1102 38-39 -1.1846033019749171 1102 40-41 -1.2315631052732883 1102 42-43 -1.2877777676307502 1102 44-45 -1.3689230590516885 1102 46-47 -1.4860200960046583 1102 48-49 -1.4435699709072978 1102 50-51 -1.1902075172375888 1102 52-53 -0.900086407182549 1102 54-55 -1.0582486759619698 1102 56-57 -1.189823564389222 1102 58-59 -1.0070952398885709 1102 60-61 -1.1631287164971909 1102 62-63 -1.1600614957407984 1102 64-65 -1.196668152353027 1102 66-67 -1.1228231799383366 1102 68-69 -1.166036015407073 1102 70-71 -1.2166827655000532 1102 72-73 -1.1546952387771086 1102 74-75 -1.314763798881792 1102 76-77 -1.166207452857556 1102 78-79 -1.1799772390701655 1102 80-81 -1.2417688769898945 1102 82-83 -1.4178258526739675 1102 84-85 -1.6611422943995926 1102 86-87 -1.5660440202068777 1102 88-89 -1.6164066713205578 1102 90-91 -1.6845521474586391 1102 92-93 -1.7362566724271922 1102 94-95 -1.7148194046133582 1102 96-97 -2.074898969964668 1102 98-99 -2.09976494103757 1102 100 -2.048376518446137 1103 1 -0.3377403354204773 1103 2 -0.5492069384855611 1103 3 -0.49871441969885666 1103 4 -0.22639063709403473 1103 5 -0.305082632924325 1103 6 -0.2750918546008023 1103 7 -0.42708382566374326 1103 8 -0.3406625401128949 1103 9 -0.5219835110912499 1103 10-11 -0.5413565621575458 1103 12-13 -0.6510424549729024 1103 14-15 -0.8523474123862869 1103 16-17 -0.7613029075529667 1103 18-19 -0.7951149850457213 1103 20-21 -0.6873339565347649 1103 22-23 -0.7355683584007409 1103 24-25 -0.6597445656392367 1103 26-27 -0.7414735621516897 1103 28-29 -0.7485880462540422 1103 30-31 -0.8735367176051696 1103 32-33 -0.881855154533902 1103 34-35 -0.9673948747941381 1103 36-37 -0.9726562933392984 1103 38-39 -1.0045573631956657 1103 40-41 -1.0576956358446594 1103 42-43 -1.1708970303558388 1103 44-45 -1.0873387111064048 1103 46-47 -1.3196015307590514 1103 48-49 -1.302016105282 1103 50-51 -1.297961481336941 1103 52-53 -1.001763351853569 1103 54-55 -0.9793587928638416 1103 56-57 -0.9492297757396173 1103 58-59 -0.8675541407214951 1103 60-61 -0.9329344466593383 1103 62-63 -0.9798118680620149 1103 64-65 -0.9911421074526459 1103 66-67 -1.0560846867266847 1103 68-69 -1.0286633519678645 1103 70-71 -1.0520825773893563 1103 72-73 -1.0019604644941609 1103 74-75 -1.0371093954817319 1103 76-77 -1.1542775619680725 1103 78-79 -1.17044793514739 1103 80-81 -1.1445242794237025 1103 82-83 -1.1280697134882836 1103 84-85 -1.1896810029556129 1103 86-87 -1.4555848375686153 1103 88-89 -1.4032975828954513 1103 90-91 -1.3600172277190552 1103 92-93 -1.5275062671911108 1103 94-95 -1.484349754597556 1103 96-97 -1.5188556657271697 1103 98-99 -1.6466030738540098 1103 100 -1.4539372366755146 1104 1 -0.46903108229557233 1104 2 -0.4338183926308652 1104 3 -0.4566510098229024 1104 4 -0.2560441145225667 1104 5 -0.2821536083212379 1104 6 -0.31575446942353835 1104 7 -0.30690156657451695 1104 8 -0.3391273833171695 1104 9 -0.5679716024576962 1104 10-11 -0.45143451129264633 1104 12-13 -0.3835585763927227 1104 14-15 -0.6882354565442412 1104 16-17 -0.714356835150177 1104 18-19 -0.7091373948110729 1104 20-21 -0.760128295426675 1104 22-23 -0.6907475772997813 1104 24-25 -0.6552746505633635 1104 26-27 -0.7084083536244279 1104 28-29 -0.9023012898772294 1104 30-31 -0.8829962617050882 1104 32-33 -0.8719575706922313 1104 34-35 -0.9338931611135521 1104 36-37 -1.0767141771054725 1104 38-39 -1.0250582142102047 1104 40-41 -0.8989021656434701 1104 42-43 -0.9748663740532848 1104 44-45 -0.9025998406521509 1104 46-47 -1.0320630822143144 1104 48-49 -1.0006540841631093 1104 50-51 -0.9946649125743576 1104 52-53 -0.8034288804618726 1104 54-55 -0.8543638939494471 1104 56-57 -0.6910123673002673 1104 58-59 -0.6520685406741151 1104 60-61 -0.8231786360539175 1104 62-63 -0.7375162692729944 1104 64-65 -0.6638836224006184 1104 66-67 -0.7289893227410644 1104 68-69 -0.8107064961826893 1104 70-71 -0.8592401138042902 1104 72-73 -0.8092995938732628 1104 74-75 -0.8881354156221235 1104 76-77 -0.9539493191866377 1104 78-79 -1.019368102106064 1104 80-81 -0.825494825897465 1104 82-83 -0.884917359662662 1104 84-85 -1.0903798317918252 1104 86-87 -0.8889771635124681 1104 88-89 -1.1040521570772874 1104 90-91 -1.0982135429562554 1104 92-93 -0.9626364292766176 1104 94-95 -1.2104477725117704 1104 96-97 -1.2173787778224892 1104 98-99 -1.4033434693481368 1104 100 -1.3790645535203971 1105 1 -0.6640882148568998 1105 2 -0.608675265504445 1105 3 -0.7122745204213352 1105 4 -0.5616323154963325 1105 5 -0.5301069472722588 1105 6 -0.5774811334988925 1105 7 -0.4933666714193592 1105 8 -0.6110780095015613 1105 9 -0.608912252230219 1105 10-11 -0.6772621616945571 1105 12-13 -0.7802401231222902 1105 14-15 -0.8524478976239109 1105 16-17 -0.9212747215008434 1105 18-19 -0.9855413538320192 1105 20-21 -0.9234799108291512 1105 22-23 -0.9912482550914277 1105 24-25 -1.017408538214191 1105 26-27 -1.0024197521358573 1105 28-29 -0.9643142824933548 1105 30-31 -0.9836765791408411 1105 32-33 -0.9457887443992448 1105 34-35 -1.08223575738986 1105 36-37 -1.217558435431343 1105 38-39 -1.1954312961958422 1105 40-41 -1.189929475143792 1105 42-43 -1.0865522197682296 1105 44-45 -1.0578150419284427 1105 46-47 -1.2470412661349997 1105 48-49 -1.3106530452749823 1105 50-51 -1.325239744577921 1105 52-53 -1.0457502228470617 1105 54-55 -1.044108868056675 1105 56-57 -1.226542627117162 1105 58-59 -1.0490790686452982 1105 60-61 -0.9944931175487426 1105 62-63 -1.1052351806599532 1105 64-65 -1.133953358195022 1105 66-67 -1.1397884334345392 1105 68-69 -1.0639439356080231 1105 70-71 -0.9776229592811632 1105 72-73 -1.0749559180219066 1105 74-75 -1.1422816250119112 1105 76-77 -1.3053577440550796 1105 78-79 -1.394661010843116 1105 80-81 -1.3221657551674681 1105 82-83 -1.3588312672660798 1105 84-85 -1.324082745673028 1105 86-87 -1.3238128197531864 1105 88-89 -1.1920080949629757 1105 90-91 -1.28158912707368 1105 92-93 -1.4957109586997213 1105 94-95 -1.3099990848423424 1105 96-97 -1.3466344047617618 1105 98-99 -1.3433831093876876 1105 100 -1.3163616546110681 1106 1 -0.41675017237287193 1106 2 -0.5718817407347281 1106 3 -0.5649506150615657 1106 4 -0.3485949627847589 1106 5 -0.33150936799329855 1106 6 -0.4015005912203975 1106 7 -0.405396756839302 1106 8 -0.40603639833276617 1106 9 -0.39184767481383176 1106 10-11 -0.5163061659399659 1106 12-13 -0.6754141340268802 1106 14-15 -0.8277197392278381 1106 16-17 -0.8771144278805281 1106 18-19 -0.7228005377049485 1106 20-21 -0.6800847426665371 1106 22-23 -0.6424523905545385 1106 24-25 -0.7685823300327215 1106 26-27 -0.872926730555541 1106 28-29 -0.80449426146874 1106 30-31 -0.8427494694562156 1106 32-33 -0.9075229680377461 1106 34-35 -0.8142427802903072 1106 36-37 -0.9109609870641862 1106 38-39 -1.0710774060742594 1106 40-41 -1.0117093739525629 1106 42-43 -0.892043440973957 1106 44-45 -1.0770992265802093 1106 46-47 -1.048374350840895 1106 48-49 -1.1080771082799927 1106 50-51 -1.1029014124909153 1106 52-53 -0.92974583977621 1106 54-55 -0.8559842323800453 1106 56-57 -0.9504543750224386 1106 58-59 -0.7750223674178329 1106 60-61 -0.7173732602650915 1106 62-63 -0.909885934645537 1106 64-65 -1.0051468362683664 1106 66-67 -0.9477435855728302 1106 68-69 -0.8531878108985751 1106 70-71 -0.7695337687602972 1106 72-73 -0.8500106529856879 1106 74-75 -0.8803074414027208 1106 76-77 -1.0421912396598074 1106 78-79 -1.0153655097439156 1106 80-81 -1.0960320494628064 1106 82-83 -0.9710530979775562 1106 84-85 -0.9738409468639837 1106 86-87 -0.9773416517826696 1106 88-89 -0.9405441378255688 1106 90-91 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38-39 1.4145703429594292 2310 40-41 1.3957176489492724 2310 42-43 1.4567617379091402 2310 44-45 1.5324490962361281 2310 46-47 1.5861010125430184 2310 48-49 1.5631827491929684 2310 50-51 1.4754127653110487 2310 52-53 1.3249411854575115 2310 54-55 1.2890290875744554 2310 56-57 1.2845839949260522 2310 58-59 1.401496752193239 2310 60-61 1.370243068515201 2310 62-63 1.3766069866995991 2310 64-65 1.2366721236445812 2310 66-67 1.298339128871369 2310 68-69 1.3063466001615822 2310 70-71 1.2718205384960726 2310 72-73 1.2020448421138354 2310 74-75 1.1188200044165129 2310 76-77 1.2693521614863457 2310 78-79 1.3051597628381728 2310 80-81 1.35738803434338 2310 82-83 1.3775510449876016 2310 84-85 1.4347236160507215 2310 86-87 1.457087869052284 2310 88-89 1.5035118324818804 2310 90-91 1.5863730426635492 2310 92-93 1.6513465774517577 2310 94-95 1.6891824182810566 2310 96-97 1.7236163679828707 2310 98-99 1.6931547875220545 2310 100 1.955301313448821 2311 1 0.5731368279374465 2311 2 0.685493627937678 2311 3 0.7125649996407759 2311 4 0.5591381849402168 2311 5 0.5586395194731111 2311 6 0.5947760162127409 2311 7 0.6460771377866266 2311 8 0.5579409420592611 2311 9 0.7781228778432805 2311 10-11 0.6415222930598361 2311 12-13 0.8101600534288664 2311 14-15 1.0181685395220015 2311 16-17 0.994734918134057 2311 18-19 1.0685461096695974 2311 20-21 1.0576467668121552 2311 22-23 1.1631595944764967 2311 24-25 1.181275498060046 2311 26-27 1.1405110426157705 2311 28-29 1.1364667906105197 2311 30-31 1.217481896653588 2311 32-33 1.2336155666769102 2311 34-35 1.2961241475727192 2311 36-37 1.367661471130603 2311 38-39 1.4396900881896428 2311 40-41 1.4503380694453085 2311 42-43 1.491924059221219 2311 44-45 1.4598832005933176 2311 46-47 1.4771377172458386 2311 48-49 1.485422671709074 2311 50-51 1.4367241830259019 2311 52-53 1.2412609438496958 2311 54-55 1.288973006176306 2311 56-57 1.2246075147777233 2311 58-59 1.2267218646421583 2311 60-61 1.3341157751865964 2311 62-63 1.3410832121926006 2311 64-65 1.214411885928122 2311 66-67 1.2346411043100076 2311 68-69 1.1530958104439293 2311 70-71 1.1651366945168036 2311 72-73 1.2364846862304404 2311 74-75 1.180390297871007 2311 76-77 1.14692088981203 2311 78-79 1.19005011798221 2311 80-81 1.2650185380458936 2311 82-83 1.3042479364758144 2311 84-85 1.3847856346376979 2311 86-87 1.308443980207901 2311 88-89 1.3408532451608615 2311 90-91 1.3851408256170963 2311 92-93 1.5233146053322244 2311 94-95 1.5269417947474722 2311 96-97 1.504981606672164 2311 98-99 1.6066075274989373 2311 100 1.6904068380387969 2312 1 0.6048046671851033 2312 2 0.5679676160198319 2312 3 0.6343159932118674 2312 4 0.4508094368061748 2312 5 0.5149364081120567 2312 6 0.41429906776286884 2312 7 0.5749889087309938 2312 8 0.6294453074502755 2312 9 0.778914745456575 2312 10-11 0.8051063875696229 2312 12-13 0.88013950334512 2312 14-15 1.1217786920757646 2312 16-17 1.084785884286923 2312 18-19 1.0459615418842816 2312 20-21 1.0447933101630653 2312 22-23 1.1411600061074907 2312 24-25 1.0856010060660353 2312 26-27 1.1075474167503003 2312 28-29 1.1243736641758701 2312 30-31 1.0120732399022145 2312 32-33 1.222344951337199 2312 34-35 1.2560679680947473 2312 36-37 1.2758689039734676 2312 38-39 1.3835603835212922 2312 40-41 1.364886950911746 2312 42-43 1.4048205526200306 2312 44-45 1.397418519261926 2312 46-47 1.4102873708565014 2312 48-49 1.3903507514218845 2312 50-51 1.3398633556361617 2312 52-53 1.2624522522855983 2312 54-55 1.2015758298962638 2312 56-57 1.1546096501671315 2312 58-59 1.2106522011740992 2312 60-61 1.2394256648142843 2312 62-63 1.2083866387274114 2312 64-65 1.2228888312314012 2312 66-67 1.1874063048714163 2312 68-69 1.2163742424816917 2312 70-71 1.2332742459188353 2312 72-73 1.3083960700013932 2312 74-75 1.2242287840529755 2312 76-77 1.121159554834648 2312 78-79 1.2069144738419268 2312 80-81 1.0725659238815837 2312 82-83 1.2948826877716009 2312 84-85 1.343614168303116 2312 86-87 1.3237751607979114 2312 88-89 1.4110672231939567 2312 90-91 1.4720460613831072 2312 92-93 1.4075823991831875 2312 94-95 1.6010138121498194 2312 96-97 1.6515951479587692 2312 98-99 1.4429413356334138 2312 100 1.6356920255161178 2313 1 0.5808864425556948 2313 2 0.559259050006883 2313 3 0.6812661993206142 2313 4 0.5230938160744003 2313 5 0.5702278194880535 2313 6 0.7273796264793404 2313 7 0.6893784546465085 2313 8 0.6815819248374737 2313 9 0.8277395323042782 2313 10-11 0.7180477848792037 2313 12-13 0.8900256366799013 2313 14-15 1.1270325918454986 2313 16-17 1.0566956783008763 2313 18-19 1.1090237808187737 2313 20-21 1.1109716354378776 2313 22-23 1.104465810248719 2313 24-25 1.171817519210748 2313 26-27 1.1168651715246938 2313 28-29 1.1369308665318059 2313 30-31 1.219213377513931 2313 32-33 1.2988998217210366 2313 34-35 1.2500524962030113 2313 36-37 1.3253420008479964 2313 38-39 1.3266884730241415 2313 40-41 1.381830491584104 2313 42-43 1.3767701917289443 2313 44-45 1.2893975017326298 2313 46-47 1.338290455335617 2313 48-49 1.4751477658247865 2313 50-51 1.370997678621471 2313 52-53 1.244690956528622 2313 54-55 1.1890977662410336 2313 56-57 1.2303059207972211 2313 58-59 1.2226436100752807 2313 60-61 1.3005223617593131 2313 62-63 1.2172492770366006 2313 64-65 1.182516202385088 2313 66-67 1.1687876502114065 2313 68-69 1.229876387119802 2313 70-71 1.1772281560358806 2313 72-73 1.1940686700255085 2313 74-75 1.1604105568246794 2313 76-77 1.1958022053414012 2313 78-79 1.1782342778123365 2313 80-81 1.3299190500705151 2313 82-83 1.3670967866344519 2313 84-85 1.4655052082373494 2313 86-87 1.5414170660359368 2313 88-89 1.4921389969631527 2313 90-91 1.5487981580933585 2313 92-93 1.6108186481273457 2313 94-95 1.615185430212943 2313 96-97 1.7181547590816741 2313 98-99 1.8011089286525817 2313 100 1.9763276044146778 2314 1 0.5458503778359933 2314 2 0.596858807062862 2314 3 0.6263001141052271 2314 4 0.599080915377364 2314 5 0.5981845133034795 2314 6 0.49439239693078463 2314 7 0.6591773671297503 2314 8 0.6414912717311978 2314 9 0.8125304052797802 2314 10-11 0.8002165926756817 2314 12-13 0.5811485845818041 2314 14-15 1.0104558589237982 2314 16-17 1.0466930136308576 2314 18-19 0.9519773719641407 2314 20-21 1.064586446385512 2314 22-23 0.9600150942171197 2314 24-25 0.87264760059184 2314 26-27 1.065279156397466 2314 28-29 1.2271862416071997 2314 30-31 1.1255599369998421 2314 32-33 1.1842905917767226 2314 34-35 1.2241113495555709 2314 36-37 1.2209075118690933 2314 38-39 1.25586145560613 2314 40-41 1.3343381810313488 2314 42-43 1.4668858142329455 2314 44-45 1.4687246331664667 2314 46-47 1.458249736950357 2314 48-49 1.5453130975986156 2314 50-51 1.5081584102404406 2314 52-53 1.319909518549359 2314 54-55 1.3386941678771578 2314 56-57 1.2795175682572975 2314 58-59 1.30198164737061 2314 60-61 1.2444581527187992 2314 62-63 1.278846309392776 2314 64-65 1.1407685509820524 2314 66-67 1.212518185523514 2314 68-69 1.2423366055113831 2314 70-71 1.2308537574031533 2314 72-73 1.2197882022333175 2314 74-75 1.2677619563129028 2314 76-77 1.3483362291167182 2314 78-79 1.3439154330676644 2314 80-81 1.3979785183887188 2314 82-83 1.2776007813985508 2314 84-85 1.2436726174215984 2314 86-87 1.4552367550681318 2314 88-89 1.5664552109912968 2314 90-91 1.5981438539382538 2314 92-93 1.697121768874247 2314 94-95 1.8202920557207314 2314 96-97 1.7829236247620663 2314 98-99 1.9876644868187796 2314 100 1.7397615410381775 2315 1 0.40362305437951207 2315 2 0.5323054025065623 2315 3 0.5750455128473178 2315 4 0.402924383940622 2315 5 0.3291590115814671 2315 6 -0.3781045773326852 2315 7 0.19118929192313772 2315 8 0.23767932040558293 2315 9 0.4867008713126708 2315 10-11 0.6270203143792941 2315 12-13 0.5473238057132264 2315 14-15 0.7339636048910521 2315 16-17 0.6375849678003789 2315 18-19 0.7390047461968763 2315 20-21 0.8172293179463708 2315 22-23 0.6984286276154492 2315 24-25 0.7434967981008569 2315 26-27 0.8477910121362271 2315 28-29 0.9358764593049571 2315 30-31 0.9662582113425415 2315 32-33 1.13496420803515 2315 34-35 1.0061528983731023 2315 36-37 1.1153233356471475 2315 38-39 1.1419371291202367 2315 40-41 1.1983526054113653 2315 42-43 1.3259326643206677 2315 44-45 1.3147943900478865 2315 46-47 1.3911662364285675 2315 48-49 1.363802002686505 2315 50-51 1.2620964986178222 2315 52-53 1.0792845347233992 2315 54-55 0.8918875423381749 2315 56-57 0.9041414793530933 2315 58-59 0.9592433333330774 2315 60-61 0.9235800530584086 2315 62-63 1.0503722830561344 2315 64-65 1.1180681720397132 2315 66-67 0.9926245705018033 2315 68-69 1.1448283468782563 2315 70-71 1.1616686536972765 2315 72-73 1.185242776414185 2315 74-75 1.0052079758928159 2315 76-77 0.9510199069195622 2315 78-79 1.0001556690574063 2315 80-81 1.0642074986113101 2315 82-83 1.1712979331609965 2315 84-85 1.085533175028818 2315 86-87 1.1684861441322205 2315 88-89 1.3097159157515996 2315 90-91 1.3550665457228241 2315 92-93 1.4893475123827713 2315 94-95 1.4388488304694604 2315 96-97 1.60193786254478 2315 98-99 1.6701334251782 2315 100 1.747203095903096 2316 1 0.12560639190952827 2316 2 0.1290241487507302 2316 3 0.12174762341721745 2316 4 0.24050424732712372 2316 5 0.20643909394414806 2316 6 0.3085780301585359 2316 7 0.23987612446843087 2316 8 0.25152475929512974 2316 9 0.42758656433069575 2316 10-11 0.2889184927565367 2316 12-13 0.41272849262563227 2316 14-15 0.6506799552828753 2316 16-17 0.6920438801806128 2316 18-19 0.6123076020292189 2316 20-21 0.5854087341859113 2316 22-23 0.4694945927597374 2316 24-25 0.5606850552194231 2316 26-27 0.5725160506452269 2316 28-29 0.7697590719976972 2316 30-31 0.7591290597960878 2316 32-33 0.7965032055889836 2316 34-35 0.9001675627232544 2316 36-37 1.019482904404228 2316 38-39 1.0752010497250524 2316 40-41 0.9730359595048057 2316 42-43 0.9506216074227254 2316 44-45 0.9042222712803394 2316 46-47 0.9962333262048517 2316 48-49 1.0520163991582976 2316 50-51 0.7656465042199656 2316 52-53 0.4720352359108304 2316 54-55 0.4490622466034324 2316 56-57 0.5495830813061318 2316 58-59 0.6864159438545698 2316 60-61 0.7832648395405428 2316 62-63 0.7915680839571309 2316 64-65 0.8149864478614148 2316 66-67 0.7626099392522434 2316 68-69 0.7488014095643436 2316 70-71 0.7052510335168449 2316 72-73 0.8583738769549996 2316 74-75 0.9809169032142933 2316 76-77 1.0355490976350623 2316 78-79 1.0694674539056592 2316 80-81 1.0558029037763461 2316 82-83 1.1043197728524667 2316 84-85 1.1254627009797495 2316 86-87 1.1840567871650656 2316 88-89 1.244064767351535 2316 90-91 1.3138871769011864 2316 92-93 1.443941217727346 2316 94-95 1.5134250542357641 2316 96-97 1.6176578194562161 2316 98-99 1.7362197741133407 2316 100 1.4589497471280737 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 25.0 1 185.0 2 99.0 3 74.0 4 60.0 5 46.0 6 71.0 7 614.0 8 1842.0 9 2942.0 10 3917.0 11 4956.0 12 5603.0 13 6345.0 14 7118.0 15 8275.0 16 9593.0 17 11707.0 18 13993.0 19 16175.0 20 19088.0 21 22110.0 22 25211.0 23 27615.0 24 30670.0 25 34270.0 26 40104.0 27 47275.0 28 56632.0 29 67987.0 30 82192.0 31 101282.0 32 128021.0 33 165629.0 34 232489.0 35 297911.0 36 431734.0 37 627819.0 38 555619.0 39 112917.0 40 61.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.04210761028601 17.38107049085042 11.212024983464614 24.364796915398955 2 16.287460229091696 19.489693126779656 41.561714028744895 22.66113261538375 3 18.021925129511413 28.307795688422498 29.50156663879795 24.168712543268143 4 12.121455526044535 15.943358426382645 37.39629036260633 34.538895684966484 5 12.587332371699201 38.32794432732234 33.2161035689337 15.868619732044763 6 29.94623365116248 35.44783457335369 21.01789950129265 13.588032274191184 7 29.427909463553735 32.05398543587627 22.37359572518893 16.144509375381066 8 28.628446423429505 34.39005883075707 20.030814473172867 16.950680272640557 9 30.467314202364125 14.755492283746555 19.094863010593965 35.682330503295354 10-11 26.307418818402905 24.828753874657043 28.950071299472945 19.9137560074671 12-13 26.092782618889455 23.088288851549375 27.856527685514436 22.962400844046734 14-15 24.15640665254596 24.569901395724468 25.69360383328067 25.580088118448895 16-17 23.527763583317316 26.502927705138603 26.503349632220225 23.465959079323852 18-19 23.233842981838333 26.666965660178455 27.333590967812306 22.765600390170906 20-21 24.35944718320534 26.706252553100583 26.25781323008519 22.676487033608883 22-23 23.753617431055883 26.323536720031232 26.375748998496302 23.547096850416587 24-25 23.220048553968432 25.867247793232295 26.759925082805065 24.15277856999421 26-27 23.261633348236213 25.987656915829156 26.677992021203867 24.072717714730768 28-29 23.160950039598525 27.277259069375244 26.536919765946763 23.024871125079464 30-31 25.057365219081696 26.068311959280855 26.812700293842433 22.06162252779502 32-33 24.673733068180486 25.963829296784958 26.602337147835737 22.760100487198823 34-35 24.249785467688252 26.549231160890564 26.729811810115113 22.471171561306075 36-37 23.9336252179289 26.490042938791124 26.44889508719969 23.127436756080286 38-39 23.738689603655583 26.978674726284503 26.299503983447366 22.983131686612555 40-41 23.702300341667083 25.889075951734974 27.097170631476974 23.31145307512097 42-43 23.420139304023756 26.290239169258278 26.230287833614103 24.059333693103863 44-45 22.528203452171006 26.56489556630181 27.218533243866293 23.688367737660887 46-47 22.465650174644765 25.511859368840422 27.106952371514254 24.91553808500056 48-49 22.87435550000164 25.852243642295797 27.32356443765182 23.94983642005074 50-51 22.78908954572385 26.108254668953208 27.41635392883784 23.686301856485102 52-53 22.598363510293552 26.465800591347126 26.67123713344825 24.264598764911067 54-55 22.307818313569086 25.851987293028735 27.620187603512985 24.220006789889194 56-57 22.927215236865013 25.839703050633606 26.97197334808472 24.261108364416657 58-59 22.53775853234505 26.132458972932902 27.56211075752787 23.767671737194174 60-61 23.521330361970726 26.263709473249925 27.03009598584748 23.18486417893187 62-63 23.181763910142926 25.848739385483242 27.22903289124934 23.740463813124492 64-65 22.61021511509969 25.41743544093391 27.134530469716257 24.83781897425014 66-67 22.620029638761572 25.499962646783292 26.841865235220553 25.038142479234583 68-69 21.997266373819084 25.776156809175234 27.329165947051425 24.89741086995426 70-71 22.093031070950342 25.217647640821333 27.886876213397617 24.80244507483071 72-73 22.03774534552747 25.90411106647639 27.096196861015287 24.96194672698086 74-75 22.46472934629659 25.66367089849916 27.03713068879747 24.834469066406783 76-77 22.056099623344895 25.021975324191704 27.477132770650435 25.444792281812962 78-79 21.73554373785307 25.235640858594234 27.788220840220205 25.24059456333249 80-81 22.116156362868068 25.553565333930088 26.919265067516125 25.41101323568572 82-83 22.675066847917723 25.45340241876089 27.074020033982226 24.797510699339153 84-85 22.471935569862392 25.680113779657855 26.48081843350462 25.36713221697513 86-87 21.80085455554611 25.888526504222774 27.190892408006523 25.119726532224597 88-89 21.809063067551236 25.00122671545608 27.566280920652293 25.62342929634039 90-91 22.85037630174149 24.721289802347822 27.21858630561702 25.209747590293663 92-93 22.71861601208039 24.78691247295922 27.21119477003987 25.283276744920517 94-95 22.46586393206183 24.36963548447642 27.257939166513122 25.90656141694863 96-97 22.38368322367321 24.297757945084612 27.42226664678813 25.89629218445405 98-99 22.253465743906133 24.586472228881785 26.9806807302107 26.17938129700138 100 22.32324204769725 24.407816552692466 26.987505243396757 26.281436156213527 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 127.0 1 97.5 2 232.5 3 354.5 4 255.5 5 208.0 6 257.0 7 418.0 8 618.0 9 760.0 10 758.0 11 714.5 12 774.0 13 866.0 14 954.0 15 1021.0 16 1101.5 17 1203.5 18 1300.5 19 1406.5 20 1523.0 21 1739.5 22 2009.5 23 2321.0 24 2700.5 25 3340.5 26 4627.5 27 6261.0 28 8026.5 29 11086.5 30 15737.0 31 20932.0 32 28422.0 33 37170.5 34 46781.0 35 56853.0 36 69131.5 37 82395.5 38 89256.0 39 92632.5 40 93970.0 41 94506.5 42 96200.5 43 99435.5 44 106178.5 45 114500.0 46 121280.5 47 126231.5 48 133750.5 49 144185.5 50 154964.5 51 159624.5 52 153898.5 53 144957.5 54 137027.5 55 124985.0 56 111172.0 57 99430.5 58 87251.0 59 72273.0 60 55620.5 61 42433.5 62 33101.5 63 25556.0 64 18797.5 65 13445.0 66 9678.0 67 7364.5 68 5571.0 69 4048.5 70 3115.5 71 2348.0 72 1809.0 73 1434.0 74 1080.5 75 778.0 76 554.5 77 378.5 78 265.0 79 198.5 80 138.0 81 98.5 82 72.0 83 51.0 84 38.5 85 32.0 86 24.5 87 23.5 88 19.0 89 14.0 90 14.0 91 11.0 92 9.0 93 6.0 94 2.0 95 2.0 96 4.0 97 3.5 98 1.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22260580024972845 2 0.1495808486518038 3 0.02346672599488294 4 0.02243556493252457 5 0.026903929536077512 6 0.05646387999035084 7 0.06268209366942101 8 0.05558895545259221 9 0.043090033484611955 10-11 0.04513673195686872 12-13 0.04377747419285087 14-15 0.02246681223744452 16-17 0.02096694160128689 18-19 0.0203888664602678 20-21 0.02296676911616373 22-23 0.0261539942179987 24-25 0.02323237120798331 26-27 0.020951317948826913 28-29 0.020685715857007334 30-31 0.018810877561810293 32-33 0.02698204779837739 34-35 0.027325768152496845 36-37 0.026185241522918644 38-39 0.024560381667081212 40-41 0.02551342446713971 42-43 0.031700390841289935 44-45 0.04163703380583424 46-47 0.04677721546516613 48-49 0.043168151746911826 50-51 0.030544240559251763 52-53 0.04618351667168707 54-55 0.04380872149777082 56-57 0.046292882238906895 58-59 0.0498394513473213 60-61 0.026435219962278253 62-63 0.027622617549236377 64-65 0.02871627322143465 66-67 0.03396582044798636 68-69 0.03838731409415938 70-71 0.04235572181899311 72-73 0.045964785537247414 74-75 0.027310144500036874 76-77 0.02076383411930721 78-79 0.0203263718504279 80-81 0.023247994860443287 82-83 0.020701339509467308 84-85 0.012717653102419917 86-87 0.015123695581256116 88-89 0.021091930820966692 90-91 0.02237307032268467 92-93 0.02220121014562494 94-95 0.026169617870458673 96-97 0.0264039726573583 98-99 0.03234096059214893 100 0.03168476718882997 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3200276.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.40975438822507 #Duplication Level Percentage of deduplicated Percentage of total 1 83.76422081293227 53.11468668269086 2 9.605713248013137 12.181918355604655 3 2.7621426117013566 5.254403537797007 4 1.224913224287143 3.1068578679574634 5 0.6859851941087305 2.174907633619675 6 0.4037153992792846 1.5359696586626195 7 0.2904616806415112 1.2892672680068504 8 0.20396944782475668 1.034692207141577 9 0.15628130101999665 0.8918783021835232 >10 0.7964101050109357 8.984290745252821 >50 0.059592135012792036 2.6270817939460933 >100 0.04267086843196653 5.192105155692621 >500 0.002729719468668254 1.1878719573281113 >1k 0.0011942522674681885 1.4240688341162189 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC 4217 0.1317698848474319 No Hit GTACATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG 3935 0.12295814486000584 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3214 0.10042883801272141 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 9.374191475985197E-5 2 0.0 0.0 0.0 0.0 9.374191475985197E-5 3 0.0 0.0 0.0 0.0 9.374191475985197E-5 4 0.0 0.0 0.0 3.124730491995065E-5 9.374191475985197E-5 5 0.0 0.0 0.0 3.124730491995065E-5 9.374191475985197E-5 6 3.124730491995065E-5 0.0 0.0 3.124730491995065E-5 9.374191475985197E-5 7 3.124730491995065E-5 0.0 0.0 3.124730491995065E-5 9.374191475985197E-5 8 3.124730491995065E-5 0.0 0.0 3.124730491995065E-5 9.374191475985197E-5 9 3.124730491995065E-5 0.0 0.0 3.124730491995065E-5 1.249892196798026E-4 10-11 3.124730491995065E-5 0.0 0.0 1.249892196798026E-4 1.249892196798026E-4 12-13 3.124730491995065E-5 1.5623652459975326E-5 0.0 1.5623652459975326E-4 1.5623652459975326E-4 14-15 3.124730491995065E-5 3.124730491995065E-5 0.0 2.187311344396546E-4 1.874838295197039E-4 16-17 3.124730491995065E-5 3.124730491995065E-5 0.0 2.6560209181958057E-4 2.187311344396546E-4 18-19 3.124730491995065E-5 4.687095737992598E-5 0.0 4.530859213392845E-4 2.8122574427955587E-4 20-21 3.124730491995065E-5 6.24946098399013E-5 0.0 7.65558970538791E-4 2.9684939673953116E-4 22-23 3.124730491995065E-5 6.24946098399013E-5 0.0 0.0020154511673368173 3.124730491995065E-4 24-25 3.124730491995065E-5 7.811826229987663E-5 0.0 0.005202676269171784 3.124730491995065E-4 26-27 3.124730491995065E-5 9.374191475985197E-5 0.0 0.008655503462826331 3.124730491995065E-4 28-29 3.124730491995065E-5 9.374191475985197E-5 0.0 0.01635796412559417 3.124730491995065E-4 30-31 3.124730491995065E-5 1.249892196798026E-4 0.0 0.046464742415966626 3.124730491995065E-4 32-33 3.124730491995065E-5 1.249892196798026E-4 0.0 0.12013026376475028 3.124730491995065E-4 34-35 3.124730491995065E-5 1.249892196798026E-4 0.0 0.21835616678061517 3.437203541194572E-4 36-37 3.124730491995065E-5 1.4061287213977793E-4 0.0 0.37001496120959565 3.437203541194572E-4 38-39 3.124730491995065E-5 1.5623652459975326E-4 0.0 0.6856283645535572 3.437203541194572E-4 40-41 3.124730491995065E-5 1.5623652459975326E-4 0.0 1.1370738023845441 4.374622688793092E-4 42-43 3.124730491995065E-5 1.5623652459975326E-4 0.0 1.5479914857343555 4.374622688793092E-4 44-45 3.124730491995065E-5 1.5623652459975326E-4 0.0 1.967470930632233 4.374622688793092E-4 46-47 3.124730491995065E-5 1.5623652459975326E-4 0.0 2.426493839906308 4.374622688793092E-4 48-49 3.124730491995065E-5 1.5623652459975326E-4 0.0 2.940558876796876 4.999568787192104E-4 50-51 3.124730491995065E-5 1.5623652459975326E-4 0.0 3.5306954775150645 5.312041836391611E-4 52-53 3.124730491995065E-5 1.5623652459975326E-4 0.0 4.155735317828837 5.624514885591118E-4 54-55 3.124730491995065E-5 1.718601770597286E-4 0.0 4.855893679170172 5.936987934790624E-4 56-57 3.124730491995065E-5 1.8748382951970394E-4 0.0 5.533772712103581 6.093224459390377E-4 58-59 3.124730491995065E-5 2.0310748197967926E-4 0.0 6.317580108715623 6.718170557789391E-4 60-61 3.124730491995065E-5 2.187311344396546E-4 0.0 7.093872528494417 7.18688013158865E-4 62-63 3.124730491995065E-5 2.187311344396546E-4 0.0 7.9580167460556535 8.12429927918717E-4 64-65 3.124730491995065E-5 2.187311344396546E-4 0.0 8.71909172833843 8.12429927918717E-4 66-67 3.124730491995065E-5 2.187311344396546E-4 0.0 9.509523553593503 8.12429927918717E-4 68-69 3.124730491995065E-5 2.187311344396546E-4 0.0 10.427944339800693 8.12429927918717E-4 70-71 3.124730491995065E-5 2.3435478689962992E-4 0.0 11.472182399268064 8.12429927918717E-4 72-73 3.124730491995065E-5 2.499784393596052E-4 0.0 12.366214663985232 8.12429927918717E-4 74-75 3.124730491995065E-5 2.499784393596052E-4 0.0 13.206235962148265 8.280535803786924E-4 76-77 3.124730491995065E-5 2.499784393596052E-4 0.0 14.085175778589097 8.59300885298643E-4 78-79 3.124730491995065E-5 2.499784393596052E-4 0.0 15.059248014858717 9.06171842678569E-4 80-81 3.124730491995065E-5 2.499784393596052E-4 0.0 15.96988822214084 9.530428000584949E-4 82-83 3.124730491995065E-5 2.6560209181958057E-4 0.0 16.900214231522533 9.999137574384209E-4 84-85 3.124730491995065E-5 2.8122574427955587E-4 0.0 17.83925823897689 9.999137574384209E-4 86-87 3.124730491995065E-5 2.8122574427955587E-4 0.0 18.758053992843116 9.999137574384209E-4 88 3.124730491995065E-5 2.8122574427955587E-4 0.0 19.445447830124653 9.999137574384209E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 6395 0.0 70.35906 2 ACCTGGG 6910 0.0 63.063957 3 CCTGGGG 5955 0.0 50.28486 4 GTACCTG 9905 0.0 46.325714 1 CTGGGGG 3895 0.0 44.051933 5 TAACGCA 560 0.0 42.811626 4 TGGGGGG 9845 0.0 42.40107 6 TATAACG 570 0.0 42.111237 2 GTACGTT 420 0.0 38.137062 1 ATGGGGG 8440 0.0 37.93012 5 CATGGGG 10875 0.0 37.6503 4 ATAACGC 665 0.0 36.051895 3 GTACATG 16670 0.0 35.09972 1 TACATGG 17140 0.0 33.80259 2 GTACCCG 765 0.0 33.254463 1 ACATGGG 16735 0.0 33.23063 3 GGGTTAG 1520 0.0 31.613619 1 TACGTTG 500 0.0 31.063232 2 GTATCAA 8685 0.0 30.050905 1 TATCACG 255 0.0 27.685593 2 >>END_MODULE