FastQCFastQC Report
Mon 6 Jun 2016
ERR1211422_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211422_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3200276
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC38700.12092707004020904No Hit
GTACATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG35320.1103654809772657No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG59800.069.23462
ACCTGGG65700.061.944253
CCTGGGG51500.052.3785064
CTGGGGG36600.046.9873125
TGGGGGG82000.042.572436
GTACCTG103150.040.6163941
GTACACG6700.038.599421
TATCACG2150.037.1585662
TAACGCA5000.035.715944
TACGTTG3350.035.070642
GTACGTG6850.034.3219761
ATGGGGG77150.033.984345
TACGTGG6550.033.7213552
GTACATG149950.033.1766131
GGGTTAG12700.032.95181
CATGGGG102800.032.548834
TACATGG152750.032.0886572
ACATGGG149950.031.2148553
ATAACGC6400.029.3716583
GTATCAA79700.028.4958531
Produced by FastQC (version 0.11.5)