FastQCFastQC Report
Mon 6 Jun 2016
ERR1211421_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211421_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16866161
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT302680.1794599257056778No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192090.113890766250838No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG187550.052.154032
ACCTGGG222850.043.0331573
GGTATCA207600.041.5139121
GTATCAA372850.041.3889541
GTACCTG297050.033.613111
GTACATG593550.033.382281
ATCAACG452200.033.0480353
CAACGCA452900.032.92435
CCTGGGG208500.032.4674
TATCAAC469350.032.3338662
TACATGG610550.031.91052
TCAACGC468950.031.837554
AACGCAG486700.031.1886276
ACATGGG611350.030.6348023
TGGGGGG398850.029.8784396
CATGGGG469350.029.8773544
ATGGGGG367050.029.226455
ACGCAGA538100.027.738437
CGCAGAG553600.026.687288
CTGGGGG159100.025.853755
Produced by FastQC (version 0.11.5)