FastQCFastQC Report
Mon 6 Jun 2016
ERR1211421_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211421_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16866161
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT279170.1655207726286972No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190160.1127464631696567No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG185100.050.978122
ACCTGGG217150.042.1994133
GGTATCA200850.041.432431
GTATCAA363150.040.4578671
CCTGGGG200600.032.5627174
GTACCTG299550.032.0027241
CAACGCA449800.031.9024895
TGGGGGG343850.031.7700046
ATCAACG452150.031.6472823
GTACATG585450.030.8054661
TCAACGC470400.030.529724
TATCAAC474900.030.4870912
AACGCAG489050.029.8793076
TACATGG602200.029.4423562
ACATGGG596100.028.3644053
ATGGGGG347450.028.2907435
CATGGGG451450.028.188754
CTGGGGG138250.027.529285
ACGCAGA545650.026.392457
CGCAGAG563050.025.4767048
Produced by FastQC (version 0.11.5)