##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211421_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 16866161 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.08651453048503 33.0 31.0 34.0 30.0 34.0 2 32.22291682143909 33.0 31.0 34.0 30.0 34.0 3 32.30603751499822 34.0 31.0 34.0 30.0 34.0 4 35.94965102016991 37.0 35.0 37.0 35.0 37.0 5 35.80859188999797 37.0 35.0 37.0 35.0 37.0 6 35.83347218136955 37.0 35.0 37.0 35.0 37.0 7 35.761977132792694 37.0 35.0 37.0 33.0 37.0 8 35.73977119037343 37.0 35.0 37.0 33.0 37.0 9 37.33100182074629 39.0 37.0 39.0 34.0 39.0 10-11 37.30296061445162 39.0 37.0 39.0 34.0 39.0 12-13 37.21657160156363 39.0 37.0 39.0 33.0 39.0 14-15 38.43912070446855 40.0 38.0 41.0 34.0 41.0 16-17 38.42548339838568 40.0 38.0 41.0 34.0 41.0 18-19 38.41261754823756 40.0 38.0 41.0 33.5 41.0 20-21 38.370596248903354 40.0 38.0 41.0 34.0 41.0 22-23 38.27281409800369 40.0 38.0 41.0 34.0 41.0 24-25 38.16370631111609 40.0 37.5 41.0 33.0 41.0 26-27 38.0509544228826 40.0 37.0 41.0 33.0 41.0 28-29 37.97998290778797 40.0 37.0 41.0 33.0 41.0 30-31 38.049490989680464 40.0 37.0 41.0 33.0 41.0 32-33 37.91965101009056 40.0 37.0 41.0 33.0 41.0 34-35 37.833262293654144 40.0 37.0 41.0 33.0 41.0 36-37 37.71186110461059 40.0 37.0 41.0 33.0 41.0 38-39 37.61402920913657 40.0 37.0 41.0 32.0 41.0 40-41 37.5656615634109 40.0 37.0 41.0 32.0 41.0 42-43 37.45465109102184 40.0 36.5 41.0 32.0 41.0 44-45 37.33540967028597 40.0 36.0 41.0 31.5 41.0 46-47 37.162647326798314 39.5 36.0 41.0 31.0 41.0 48-49 36.994866585229445 39.0 36.0 41.0 31.0 41.0 50-51 36.84419575385294 39.0 35.5 41.0 31.0 41.0 52-53 36.65768001384548 39.0 35.0 40.5 30.0 41.0 54-55 36.46967662054216 39.0 35.0 40.0 30.0 41.0 56-57 36.25738586866329 38.5 35.0 40.0 29.5 41.0 58-59 36.0097857479245 38.0 35.0 40.0 29.0 41.0 60-61 35.7587804065193 38.0 34.0 40.0 28.5 41.0 62-63 35.46527745703364 37.5 34.0 40.0 28.0 41.0 64-65 35.123904248275586 37.0 34.0 40.0 28.0 41.0 66-67 34.78071053039278 36.0 34.0 39.0 27.5 41.0 68-69 34.42376830744115 36.0 33.0 39.0 26.5 40.0 70-71 34.0498375712173 35.5 33.0 38.5 26.0 40.0 72-73 33.692230555607765 35.0 33.0 38.0 26.0 40.0 74-75 33.32060971076939 35.0 32.0 37.0 26.0 39.0 76-77 32.298482950565926 34.0 31.0 36.0 25.5 39.0 78-79 32.788448687285744 35.0 32.0 36.0 26.0 38.5 80-81 32.683225779713595 35.0 32.0 36.0 26.0 37.5 82-83 32.40734957409691 35.0 32.0 35.0 25.5 37.0 84-85 32.13039449226176 34.0 32.0 35.0 25.0 36.5 86-87 31.886133691004137 34.0 31.0 35.0 25.0 36.0 88-89 31.670706837199052 34.0 31.0 35.0 25.0 36.0 90-91 31.458849349297687 34.0 31.0 35.0 24.0 35.5 92-93 31.287247613727864 34.0 31.0 35.0 24.0 35.0 94-95 31.124825679062354 34.0 31.0 35.0 23.5 35.0 96-97 30.964527701354207 34.0 30.5 35.0 23.0 35.0 98-99 30.786476306018898 34.0 30.0 35.0 22.0 35.0 100 28.941680919564327 32.0 26.0 34.0 18.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3007025299052124 1101 2 -0.26949036413751415 1101 3 -0.30430162245493975 1101 4 -0.21436886562086954 1101 5 -0.1855971469596085 1101 6 -0.27857067358924326 1101 7 -0.2710662365723806 1101 8 -0.3393543900050702 1101 9 -0.43303701587475274 1101 10-11 -0.42650477623364225 1101 12-13 -0.46370391445361037 1101 14-15 -0.6149606097411677 1101 16-17 -0.621824873688503 1101 18-19 -0.628976633916082 1101 20-21 -0.6303854759710958 1101 22-23 -0.709426533104434 1101 24-25 -0.7571895832436937 1101 26-27 -0.767392118419508 1101 28-29 -0.6846258225595179 1101 30-31 -0.6488593674135643 1101 32-33 -0.5508506329525886 1101 34-35 -0.7250734940905375 1101 36-37 -0.7027592465016212 1101 38-39 -0.679651258610896 1101 40-41 -0.7310107299113113 1101 42-43 -0.7144169012103134 1101 44-45 -0.7573209720537477 1101 46-47 -0.8308617367290196 1101 48-49 -0.6978761581805983 1101 50-51 -0.7819651405175492 1101 52-53 -0.8262911266156792 1101 54-55 -0.8093810769476519 1101 56-57 -0.8654976737359519 1101 58-59 -0.8210974172934513 1101 60-61 -0.7984385036939514 1101 62-63 -0.8742590646084878 1101 64-65 -0.9331394185778876 1101 66-67 -0.8621981405839563 1101 68-69 -0.9352725410801526 1101 70-71 -0.9765909044460841 1101 72-73 -1.0737086268837785 1101 74-75 -1.0687722766143537 1101 76-77 -0.906032227320889 1101 78-79 -0.8277953288440969 1101 80-81 -0.7084089094610064 1101 82-83 -0.7990401233951303 1101 84-85 -0.9162986161233349 1101 86-87 -0.9009663355423321 1101 88-89 -0.87914730214165 1101 90-91 -1.1175636571100505 1101 92-93 -1.1186901028355862 1101 94-95 -1.1558306786643477 1101 96-97 -1.0459911434505038 1101 98-99 -1.2308166517139085 1101 100 -1.1547654450910727 1102 1 -0.31110961516122515 1102 2 -0.28180896623495855 1102 3 -0.31478649514800594 1102 4 -0.16077499526776506 1102 5 -0.16597366880158404 1102 6 -0.24390630414168157 1102 7 -0.2583287893206361 1102 8 -0.2972714297781067 1102 9 -0.41691146564690484 1102 10-11 -0.44149817703162597 1102 12-13 -0.43136525892933975 1102 14-15 -0.5426651374422775 1102 16-17 -0.6430217833010516 1102 18-19 -0.6567950173890935 1102 20-21 -0.6625169386570988 1102 22-23 -0.648914089444439 1102 24-25 -0.7225598228798447 1102 26-27 -0.7877787586004672 1102 28-29 -0.8012293517957332 1102 30-31 -0.6394083077540316 1102 32-33 -0.6699641271964012 1102 34-35 -0.7343614193735419 1102 36-37 -0.6729477279211977 1102 38-39 -0.716679819220424 1102 40-41 -0.6906008635913707 1102 42-43 -0.705344007230913 1102 44-45 -0.6774848803755944 1102 46-47 -0.7887886855795472 1102 48-49 -0.871673277700566 1102 50-51 -0.9286909667866823 1102 52-53 -0.8975509156507115 1102 54-55 -0.9827944200965035 1102 56-57 -1.013625180664782 1102 58-59 -1.049826505888646 1102 60-61 -1.1240679876644677 1102 62-63 -1.0665235711580792 1102 64-65 -1.0005669681534712 1102 66-67 -0.9906465339315531 1102 68-69 -1.0711719183036763 1102 70-71 -0.9874245809889501 1102 72-73 -1.1116518008467935 1102 74-75 -1.0648002452959773 1102 76-77 -0.9041390139836274 1102 78-79 -0.8570559820827057 1102 80-81 -0.7110886017999434 1102 82-83 -0.7732419678304225 1102 84-85 -0.8037651952841784 1102 86-87 -0.9146826008404112 1102 88-89 -0.8799926894696135 1102 90-91 -0.8708432886916491 1102 92-93 -0.9405368357628383 1102 94-95 -0.9640769218018512 1102 96-97 -1.0761332409576632 1102 98-99 -1.068302612118785 1102 100 -1.1259978490034932 1103 1 -0.352960766239768 1103 2 -0.3141834413377893 1103 3 -0.33754726128252344 1103 4 -0.201250898151919 1103 5 -0.16382089658753074 1103 6 -0.29564553530713766 1103 7 -0.3085376320418618 1103 8 -0.3566051417311087 1103 9 -0.450345777385742 1103 10-11 -0.45262846515120714 1103 12-13 -0.5222348671848565 1103 14-15 -0.6845436039808206 1103 16-17 -0.738033605312495 1103 18-19 -0.6566338226505835 1103 20-21 -0.6667565361331711 1103 22-23 -0.7685876465113353 1103 24-25 -0.7005066028403917 1103 26-27 -0.8053462489352157 1103 28-29 -0.8361044290853172 1103 30-31 -0.7195259993051693 1103 32-33 -0.7398087265794544 1103 34-35 -0.7615964024499888 1103 36-37 -0.7058701382637267 1103 38-39 -0.7621594980297957 1103 40-41 -0.824123278579151 1103 42-43 -0.817734423946952 1103 44-45 -0.8211786074816843 1103 46-47 -0.871077640026158 1103 48-49 -0.8889591178796152 1103 50-51 -0.9350152254867155 1103 52-53 -1.019960997209452 1103 54-55 -0.9638615803351414 1103 56-57 -1.023721618672191 1103 58-59 -1.058085394539475 1103 60-61 -0.973085868701439 1103 62-63 -1.0687513339155075 1103 64-65 -1.0178857968644621 1103 66-67 -1.0346398839509945 1103 68-69 -1.0345305724213603 1103 70-71 -1.0234841559530068 1103 72-73 -1.0301954329429819 1103 74-75 -1.0972698898944842 1103 76-77 -1.0169355451631077 1103 78-79 -0.8453410443522955 1103 80-81 -0.8058979015668157 1103 82-83 -0.8331088923374743 1103 84-85 -0.8795380143277782 1103 86-87 -0.913096931060295 1103 88-89 -0.9219511451181042 1103 90-91 -1.0353942494894177 1103 92-93 -0.974657057231056 1103 94-95 -1.0266300559121255 1103 96-97 -1.1217022435495103 1103 98-99 -1.1507560976965507 1103 100 -1.1286388787917154 1104 1 -0.3968341637117909 1104 2 -0.3321164876529643 1104 3 -0.34350159665982005 1104 4 -0.2142407097833683 1104 5 -0.21454547392477252 1104 6 -0.34021158202558865 1104 7 -0.30516771249588714 1104 8 -0.3298421664247826 1104 9 -0.463406397067871 1104 10-11 -0.48785815379577 1104 12-13 -0.5136888581921042 1104 14-15 -0.6355669207466903 1104 16-17 -0.696870295232408 1104 18-19 -0.6833974364776623 1104 20-21 -0.6474767920605018 1104 22-23 -0.7557988621550749 1104 24-25 -0.84486614250266 1104 26-27 -0.8100658945927393 1104 28-29 -0.8141292774386883 1104 30-31 -0.814120350355175 1104 32-33 -0.7411280315912947 1104 34-35 -0.8193933896421584 1104 36-37 -0.7472265895797179 1104 38-39 -0.8977004234529105 1104 40-41 -0.9656484246145993 1104 42-43 -0.8444896795168404 1104 44-45 -0.925684473927042 1104 46-47 -0.9233454378477433 1104 48-49 -0.9522685697154927 1104 50-51 -0.9186949860993323 1104 52-53 -0.8802411135951544 1104 54-55 -0.8466247618569867 1104 56-57 -0.9077166196500883 1104 58-59 -0.9983869459231656 1104 60-61 -0.995396742652396 1104 62-63 -1.0144316978994397 1104 64-65 -0.9949776842714471 1104 66-67 -1.056339137144512 1104 68-69 -0.9814595360225127 1104 70-71 -0.9836862701506561 1104 72-73 -0.9490119722976402 1104 74-75 -0.9386201181995304 1104 76-77 -0.8909695443606687 1104 78-79 -0.83892370956913 1104 80-81 -0.8486242385933416 1104 82-83 -0.9643392934972752 1104 84-85 -0.858466654931874 1104 86-87 -0.7695228499440141 1104 88-89 -0.8885445543602621 1104 90-91 -0.8872026511331761 1104 92-93 -0.9987317985695832 1104 94-95 -1.0285838185506826 1104 96-97 -1.1020130742206682 1104 98-99 -1.059510484139082 1104 100 -1.21871206558885 1105 1 -0.3609810886917053 1105 2 -0.3331533893669061 1105 3 -0.34877073148035365 1105 4 -0.20655188557076798 1105 5 -0.2774752833868277 1105 6 -0.2818262416856996 1105 7 -0.31198058597004064 1105 8 -0.3105314439500262 1105 9 -0.5056489841666618 1105 10-11 -0.4906717711524209 1105 12-13 -0.5252682953808474 1105 14-15 -0.6788863144203177 1105 16-17 -0.65104220090182 1105 18-19 -0.6117872060516305 1105 20-21 -0.7199207176349063 1105 22-23 -0.6657825536836413 1105 24-25 -0.723122387370914 1105 26-27 -0.7175440252494951 1105 28-29 -0.7023034295832744 1105 30-31 -0.6990595580325305 1105 32-33 -0.6589237880089343 1105 34-35 -0.6772430694859963 1105 36-37 -0.8152009405927103 1105 38-39 -0.7772780288153882 1105 40-41 -0.7802008454481211 1105 42-43 -0.7913393021212372 1105 44-45 -0.7396341505357498 1105 46-47 -0.8628371137019784 1105 48-49 -0.7969507180873379 1105 50-51 -0.8529229683313702 1105 52-53 -0.796230272985575 1105 54-55 -0.8873974486786409 1105 56-57 -0.8525673210583662 1105 58-59 -0.8770631945894181 1105 60-61 -0.982136312171086 1105 62-63 -0.9927995070599422 1105 64-65 -0.9471477303332492 1105 66-67 -0.9684007171093825 1105 68-69 -0.9831225248665092 1105 70-71 -0.9561814029905236 1105 72-73 -0.9552427731690472 1105 74-75 -0.9631016229581562 1105 76-77 -0.9305598596062765 1105 78-79 -0.8073366861005695 1105 80-81 -0.7511753641586445 1105 82-83 -0.7538207108022057 1105 84-85 -0.6870300135274903 1105 86-87 -0.7414077589661936 1105 88-89 -0.6881749983613936 1105 90-91 -0.7492741296405754 1105 92-93 -0.8318764406791175 1105 94-95 -0.7920305525396039 1105 96-97 -0.8679823420037032 1105 98-99 -0.9027909609156204 1105 100 -0.906033184138785 1106 1 -0.3481349130343041 1106 2 -0.3277343978982188 1106 3 -0.37255748732732386 1106 4 -0.20289291896690287 1106 5 -0.23918615764394957 1106 6 -0.25914744528227374 1106 7 -0.29247198299083266 1106 8 -0.30216514274292905 1106 9 -0.4282226906038886 1106 10-11 -0.4446443912856495 1106 12-13 -0.5019343663494524 1106 14-15 -0.625964778955229 1106 16-17 -0.6496538435899595 1106 18-19 -0.6656235967679294 1106 20-21 -0.6454650218612983 1106 22-23 -0.6588900858604276 1106 24-25 -0.711046843336085 1106 26-27 -0.7198466535827208 1106 28-29 -0.7505986111930625 1106 30-31 -0.7634029492504979 1106 32-33 -0.6835765672292524 1106 34-35 -0.699437734091326 1106 36-37 -0.7407247805459249 1106 38-39 -0.742586590457087 1106 40-41 -0.7990227024733159 1106 42-43 -0.7195645884198285 1106 44-45 -0.8254780763889329 1106 46-47 -0.8737234092018014 1106 48-49 -0.8495792460566278 1106 50-51 -0.83896815162894 1106 52-53 -0.9016281120881615 1106 54-55 -0.9215506490637679 1106 56-57 -1.0048751490372112 1106 58-59 -0.9652362176335174 1106 60-61 -1.0623603039461642 1106 62-63 -0.9734777602594491 1106 64-65 -1.0425370468206765 1106 66-67 -0.9594959507286163 1106 68-69 -0.9345180097824723 1106 70-71 -0.8762853589016828 1106 72-73 -0.8997447354023507 1106 74-75 -0.9032964318033478 1106 76-77 -0.9071140667881998 1106 78-79 -0.7429731227243508 1106 80-81 -0.723047748107227 1106 82-83 -0.7550644225416434 1106 84-85 -0.7584318749876395 1106 86-87 -0.7462337409137483 1106 88-89 -0.745415160839606 1106 90-91 -0.7891802413079319 1106 92-93 -0.7644605684784871 1106 94-95 -0.7721737810300766 1106 96-97 -0.8548979141698503 1106 98-99 -0.9178204846462563 1106 100 -0.991333532807154 1107 1 -0.4029171827081619 1107 2 -0.36101481342057085 1107 3 -0.39489475121603945 1107 4 -0.23550609347583418 1107 5 -0.2964980142882183 1107 6 -0.3324639835051997 1107 7 -0.3799971739905885 1107 8 -0.4542244617564961 1107 9 -0.530380047949663 1107 10-11 -0.47036093862797657 1107 12-13 -0.5477573688291457 1107 14-15 -0.6906843788381352 1107 16-17 -0.6871057549265274 1107 18-19 -0.722186355135122 1107 20-21 -0.7110156129819529 1107 22-23 -0.7886748983529515 1107 24-25 -0.7592871993303802 1107 26-27 -0.7797488951945866 1107 28-29 -0.7635098771101312 1107 30-31 -0.7543161563881 1107 32-33 -0.800918605635836 1107 34-35 -0.7944226015636318 1107 36-37 -0.77779440045407 1107 38-39 -0.819785597514759 1107 40-41 -0.7849767230603177 1107 42-43 -0.7698710054073743 1107 44-45 -0.8017091611656113 1107 46-47 -0.8754535234316734 1107 48-49 -0.8585191555429148 1107 50-51 -0.8526515899887599 1107 52-53 -0.8314121685968416 1107 54-55 -0.8447544592286533 1107 56-57 -0.8287776679436192 1107 58-59 -0.8938448620620036 1107 60-61 -0.8830566263806787 1107 62-63 -0.8405859037712915 1107 64-65 -0.8419576657253742 1107 66-67 -0.8458919747581461 1107 68-69 -0.8625794563709519 1107 70-71 -0.8596797038855613 1107 72-73 -0.9000627682045348 1107 74-75 -0.8254291505014635 1107 76-77 -0.8807551870290098 1107 78-79 -0.7513394919432415 1107 80-81 -0.7188267484405273 1107 82-83 -0.7280354916192415 1107 84-85 -0.7089734430088583 1107 86-87 -0.7510312337857634 1107 88-89 -0.7401165569807198 1107 90-91 -0.7511280962557514 1107 92-93 -0.8354559904053644 1107 94-95 -0.8263481817895304 1107 96-97 -0.8624949746413222 1107 98-99 -0.8599358869515044 1107 100 -0.845590454795083 1108 1 -0.46748771022351576 1108 2 -0.4000460523467275 1108 3 -0.4544198037435656 1108 4 -0.2957742311143434 1108 5 -0.34405820827166167 1108 6 -0.4081257304836683 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0.7837885349570897 2311 30-31 0.8834597861887019 2311 32-33 1.0165629703371764 2311 34-35 0.9710185510526941 2311 36-37 1.037347760301735 2311 38-39 1.082741993267696 2311 40-41 1.056030058140884 2311 42-43 1.148211326766365 2311 44-45 1.147090083044759 2311 46-47 1.1729275730578763 2311 48-49 1.131097061471273 2311 50-51 1.1925638371823268 2311 52-53 1.1568738898800248 2311 54-55 1.130935721547118 2311 56-57 1.1782479097399232 2311 58-59 1.2209228901589455 2311 60-61 1.2396204386389726 2311 62-63 1.2920077416443334 2311 64-65 1.232756674350533 2311 66-67 1.2551633690253752 2311 68-69 1.2628198736169693 2311 70-71 1.269502159324496 2311 72-73 1.2480224778306024 2311 74-75 1.1759697540522467 2311 76-77 1.049944874641831 2311 78-79 1.0216851060987509 2311 80-81 1.0417762491525835 2311 82-83 1.0710993266893993 2311 84-85 1.0961116518995198 2311 86-87 1.150849772149261 2311 88-89 1.1712147214190587 2311 90-91 1.2137273903890424 2311 92-93 1.230748158176624 2311 94-95 1.2668964043386275 2311 96-97 1.2451575223955835 2311 98-99 1.3080108619548305 2311 100 1.4582203929290003 2312 1 0.14108469213277175 2312 2 0.13717768688896825 2312 3 0.32266381657235144 2312 4 0.2338590621893175 2312 5 0.30785357669181224 2312 6 0.4114364862548854 2312 7 0.45617957965099265 2312 8 0.27280841390165733 2312 9 0.4353790681705618 2312 10-11 0.29511473703831825 2312 12-13 0.4330771715703392 2312 14-15 0.7858496397468357 2312 16-17 0.8735346255738392 2312 18-19 0.8697372103973464 2312 20-21 0.8839302698619633 2312 22-23 0.5496988925551705 2312 24-25 0.47991027784828333 2312 26-27 0.7796384126677509 2312 28-29 0.9120154200274655 2312 30-31 0.908255307485355 2312 32-33 0.7736218032181341 2312 34-35 0.9467400383416802 2312 36-37 1.037213884259863 2312 38-39 1.0481155291817643 2312 40-41 1.0537367247822118 2312 42-43 0.9143633916144722 2312 44-45 0.8789577900924712 2312 46-47 1.013689552345653 2312 48-49 1.0575832388051367 2312 50-51 1.1132912296803639 2312 52-53 1.1591062100276872 2312 54-55 1.1893961820062273 2312 56-57 1.180302209266479 2312 58-59 1.2116379066283756 2312 60-61 1.2167994175491899 2312 62-63 1.2417765574590547 2312 64-65 1.2656840640946285 2312 66-67 1.2318844609086668 2312 68-69 1.255333514405578 2312 70-71 1.1078175349336092 2312 72-73 1.2003496894551802 2312 74-75 1.2321396921608923 2312 76-77 1.1771106267060176 2312 78-79 1.081635470640414 2312 80-81 0.9362744648428389 2312 82-83 0.9375228760513679 2312 84-85 0.7969226118886112 2312 86-87 1.0159953974049323 2312 88-89 0.67993884102032 2312 90-91 0.9995367995589568 2312 92-93 1.1848801287552106 2312 94-95 1.263701570185109 2312 96-97 1.2726005395531494 2312 98-99 1.2981689023892002 2312 100 1.4016397971216925 2313 1 -0.28554515404238145 2313 2 0.03961799570186031 2313 3 0.2529552871390379 2313 4 0.0885395511297773 2313 5 0.2180860344895379 2313 6 0.3403889290499791 2313 7 0.10791004502954138 2313 8 0.08581908565935237 2313 9 0.257614872262792 2313 10-11 0.39862248744916684 2313 12-13 0.5920739166664362 2313 14-15 0.6387071589436815 2313 16-17 0.780977890482994 2313 18-19 0.6799737486914026 2313 20-21 0.8291823963632297 2313 22-23 0.905109492479582 2313 24-25 0.7131393748746788 2313 26-27 0.9398186668970681 2313 28-29 0.8519311582195925 2313 30-31 0.8483837944181474 2313 32-33 0.9829398449660758 2313 34-35 0.9650047739725736 2313 36-37 0.8559564881553356 2313 38-39 0.6316805505023879 2313 40-41 0.7686517779735311 2313 42-43 0.7611696588391723 2313 44-45 0.9744197886336678 2313 46-47 1.0278248932815046 2313 48-49 1.0219464098488018 2313 50-51 0.9540604632394221 2313 52-53 0.9542117350071067 2313 54-55 1.020496692245068 2313 56-57 1.0826491463660801 2313 58-59 1.0015028324393072 2313 60-61 0.9891372446424924 2313 62-63 1.0588771745741568 2313 64-65 1.1795162629176517 2313 66-67 1.1641855387175823 2313 68-69 1.1341992254049913 2313 70-71 1.0381970064138244 2313 72-73 1.1394598013775479 2313 74-75 0.8011066401254752 2313 76-77 0.9657828024302901 2313 78-79 0.782282949492938 2313 80-81 0.8834860328701168 2313 82-83 0.9050697976733844 2313 84-85 0.9357223112569883 2313 86-87 1.0753980153145797 2313 88-89 1.01538044172052 2313 90-91 1.109499547621187 2313 92-93 0.8541134706579498 2313 94-95 0.8782851051809004 2313 96-97 1.1881815598090917 2313 98-99 1.212785359038218 2313 100 1.352036994687559 2314 1 -0.3314973223464257 2314 2 -0.14371021696911157 2314 3 0.11969589318770346 2314 4 -0.18949523016993197 2314 5 0.1635488811084258 2314 6 0.17780639689387812 2314 7 0.11494856722694635 2314 8 0.1566653895787269 2314 9 0.19446414987843497 2314 10-11 0.42035337617900126 2314 12-13 0.5048405952700534 2314 14-15 0.5856172477014709 2314 16-17 0.7787368664493712 2314 18-19 0.8411333012659696 2314 20-21 0.4678357626674057 2314 22-23 0.6760013410221362 2314 24-25 0.8585590392514248 2314 26-27 0.8239372019109865 2314 28-29 0.8012162948622219 2314 30-31 0.7883877683086169 2314 32-33 0.8904375085464125 2314 34-35 0.9291984278884371 2314 36-37 0.8387961016622327 2314 38-39 0.8434944365619401 2314 40-41 0.8910459904288928 2314 42-43 0.6909743424762667 2314 44-45 0.7541705857352028 2314 46-47 0.8970419433804722 2314 48-49 0.774164924808268 2314 50-51 0.8882456906826306 2314 52-53 0.9485426875069862 2314 54-55 0.9924441408822631 2314 56-57 0.914161105044279 2314 58-59 0.9021847601612052 2314 60-61 0.9797095815914076 2314 62-63 0.6763691552474214 2314 64-65 0.8259943973282518 2314 66-67 0.9519192982071658 2314 68-69 0.9868349991096323 2314 70-71 1.0275963791657858 2314 72-73 0.9963347113679646 2314 74-75 1.0258810126523485 2314 76-77 0.8575113919904709 2314 78-79 0.8363680321742208 2314 80-81 0.8348044202175231 2314 82-83 0.8537371554593491 2314 84-85 0.7808384425899533 2314 86-87 0.9146764883770153 2314 88-89 0.9529308018508438 2314 90-91 1.0758951477690353 2314 92-93 1.1252439120698554 2314 94-95 1.1596016258394748 2314 96-97 1.182642272524273 2314 98-99 1.2413282813508815 2314 100 1.3093692951572393 2315 1 -0.07367678967067803 2315 2 0.11414867276642582 2315 3 0.11911613573905555 2315 4 -0.06762320578496173 2315 5 0.23587847429396902 2315 6 0.37776479425503595 2315 7 0.43246068105202085 2315 8 0.3638359449390052 2315 9 0.6148887812172887 2315 10-11 0.5681878822223609 2315 12-13 0.6438969514200252 2315 14-15 0.7207854791757384 2315 16-17 0.7459988564418225 2315 18-19 0.822451188566248 2315 20-21 0.8133069665421857 2315 22-23 0.9241092915048554 2315 24-25 0.8270922028681653 2315 26-27 0.8949841307797755 2315 28-29 0.9106724237007313 2315 30-31 0.8819472049520769 2315 32-33 0.9043715419069258 2315 34-35 0.9096717135853112 2315 36-37 0.9534466459946884 2315 38-39 0.9507793922755923 2315 40-41 0.926302304595346 2315 42-43 1.012315151696498 2315 44-45 0.8779736439600327 2315 46-47 0.9069852083066507 2315 48-49 0.9754081670277657 2315 50-51 0.8677540022144754 2315 52-53 0.9131395319557356 2315 54-55 0.8887431933260075 2315 56-57 0.9694248596185915 2315 58-59 0.9982364963386559 2315 60-61 1.0681998815211458 2315 62-63 1.071883454337005 2315 64-65 1.0560786753562397 2315 66-67 1.0790410871829081 2315 68-69 1.0090463350493408 2315 70-71 0.6080820084896885 2315 72-73 0.9102281516613431 2315 74-75 1.0610313670182094 2315 76-77 1.0516246571576957 2315 78-79 0.9204668129748512 2315 80-81 0.8873820581987815 2315 82-83 0.8611532309957042 2315 84-85 0.8078849283707896 2315 86-87 0.8368655492273156 2315 88-89 0.9195376938258271 2315 90-91 0.9841135678694322 2315 92-93 1.0147650604215244 2315 94-95 1.0814159894202398 2315 96-97 1.1461675557446078 2315 98-99 1.1031414661537227 2315 100 0.9904856730380729 2316 1 0.49753256093030984 2316 2 0.4857197088700076 2316 3 0.48784417277556 2316 4 0.31647713152781165 2316 5 0.4016730291825539 2316 6 0.40945612872928194 2316 7 0.46937128735339684 2316 8 0.4872122010609914 2316 9 0.49525773396935335 2316 10-11 0.4714381433034873 2316 12-13 0.6230697830228848 2316 14-15 0.7748915731479045 2316 16-17 0.7779104267310686 2316 18-19 0.8101436017459562 2316 20-21 0.8641267031896973 2316 22-23 0.8812513461948015 2316 24-25 0.9063038187101782 2316 26-27 0.9644107079476356 2316 28-29 0.9061353844397004 2316 30-31 0.8076301835045498 2316 32-33 0.7768533313418331 2316 34-35 0.7296254105745774 2316 36-37 0.729733547232378 2316 38-39 0.6609847124063748 2316 40-41 0.7532295660796393 2316 42-43 0.8594518491535581 2316 44-45 0.8037880298860287 2316 46-47 0.8145698429460282 2316 48-49 0.5774107947615192 2316 50-51 0.7162393718009881 2316 52-53 0.8520105587003428 2316 54-55 0.9227225774173746 2316 56-57 0.9709886372172107 2316 58-59 0.9144233093597833 2316 60-61 0.9820572460313954 2316 62-63 1.0100432893881504 2316 64-65 0.9851236523941225 2316 66-67 0.9681038158520536 2316 68-69 0.9524209403870572 2316 70-71 0.8774413996358064 2316 72-73 0.8569165572664019 2316 74-75 0.8660101122481265 2316 76-77 0.7207319509843515 2316 78-79 0.7203372452387313 2316 80-81 0.7071438814292392 2316 82-83 0.641465091284779 2316 84-85 0.6613449574808783 2316 86-87 0.7027501593740944 2316 88-89 0.6564054894186917 2316 90-91 0.7189682933297838 2316 92-93 0.7498706016139103 2316 94-95 0.6591850895285312 2316 96-97 0.7811379092096615 2316 98-99 0.8923281301092132 2316 100 0.5799020251034683 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 16.0 12 99.0 13 376.0 14 1440.0 15 4148.0 16 9728.0 17 19125.0 18 31121.0 19 43065.0 20 58727.0 21 77666.0 22 95158.0 23 105773.0 24 116965.0 25 136568.0 26 168899.0 27 211360.0 28 263015.0 29 324919.0 30 402238.0 31 502018.0 32 634466.0 33 828577.0 34 1161654.0 35 1572387.0 36 2383843.0 37 3722198.0 38 3469033.0 39 521529.0 40 50.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.57941704332475 19.71562524747615 13.19775520778802 24.507202501411076 2 15.47132747043029 20.891630288599757 40.58455270289427 23.052489538075676 3 17.36879155473395 27.9021263821834 30.598762883712883 24.130319179369767 4 11.997115173188048 17.430365779056235 38.80816708180626 31.764351965949462 5 12.608123194092741 38.27007277111051 34.51841050040426 14.60339353439249 6 29.492583343961126 37.61236924186473 19.734427974976402 13.160619439197745 7 26.94657987103505 32.70170465274998 23.554017983849647 16.79769749236532 8 26.12565025336595 35.39883332963756 20.94238875833787 17.53312765865862 9 27.928281861231273 15.61447148410307 20.201676730126636 36.255569924539024 10-11 24.77408569265812 25.88754643286006 29.458472091131878 19.87989578334994 12-13 25.27466692896782 23.94406072129438 28.735592520610638 22.045679829127163 14-15 23.016042840356064 25.439197664102775 26.34764133824708 25.197118157294078 16-17 22.44227681456276 27.543146645916615 27.152096955421285 22.86247958409934 18-19 22.20518943226025 27.389057296441084 28.413436821811437 21.99231644948723 20-21 23.168852963032805 26.901466851680166 27.724739776961037 22.20494040832599 22-23 22.850428821663353 26.720195150520475 27.740540000197438 22.688836027618734 24-25 22.37939339733629 26.526979560685092 28.133736527562085 22.959890514416525 26-27 22.482973153520017 26.675534127242607 27.875934882387867 22.965557836849516 28-29 22.383564438626827 27.453161946063897 27.78801878701212 22.375254828297155 30-31 23.43092529251292 26.65437349812555 27.831596417575305 22.083104791786223 32-33 23.17961804454717 26.728600607440097 27.56720855282202 22.524572795190718 34-35 23.119068969262607 27.003025017858718 27.908849101640833 21.969056911237843 36-37 22.81926745111366 26.985288023238347 27.719512381365845 22.47593214428214 38-39 22.77875697951186 27.03250498727707 27.793150172613647 22.395587860597427 40-41 22.923598519103297 26.391468227822827 28.162398819053507 22.522534434020365 42-43 22.669363634396237 26.705829618480653 27.649600331557455 22.975206415565648 44-45 22.102139376736876 26.919921116021456 28.134415016809406 22.843524490432262 46-47 22.07287544942865 26.420422832435627 27.833024905904065 23.673676812231665 48-49 22.29893984899947 26.509653969327086 28.05276284551912 23.138643336154324 50-51 22.20529210048683 26.759647041501992 27.861208856350167 23.17385200166101 52-53 21.85768992737584 26.935717928204024 27.67023480434671 23.536357340073426 54-55 21.746138210894006 26.639154429493605 28.101113371579956 23.513593988032433 56-57 22.235121535943815 26.540672652831027 27.60670211184626 23.617503699378897 58-59 21.749494239866742 26.63409827169319 28.071015612835176 23.54539187560489 60-61 22.36863711476264 26.88503529121168 27.502619266721528 23.243708327304148 62-63 22.28037278337919 26.685593515185708 27.427545305973183 23.60648839546192 64-65 21.816315965194537 26.430219173841703 27.509735887188835 24.243728973774928 66-67 21.78352680390005 26.3237448510789 27.366583311126448 24.526145033894608 68-69 21.50306887583845 26.514374183328016 27.607350589940722 24.375206350892814 70-71 21.708841246167808 26.21329543639586 27.770701184793396 24.30716213264294 72-73 21.69541024158459 26.60136122807338 27.26635173651305 24.43687679382898 74-75 21.88623059906413 26.693967843523335 26.99857365683547 24.421227900577062 76-77 21.599284204355826 26.43747608790506 27.183334769566432 24.779904938172688 78-79 21.410501727845773 26.441578758333623 27.457315594705733 24.690603919114864 80-81 21.552786325145114 26.704775108137362 27.11540152487128 24.62703704184624 82-83 21.958384810575364 26.369325279956477 27.23259799866743 24.439691910800725 84-85 21.973061425350586 26.620919399010184 26.73675126005039 24.669267915588843 86-87 21.45689391484172 26.65422787039169 27.124912174531175 24.763966040235417 88-89 21.544271252475138 26.22040399691056 27.17150964584434 25.063815104769965 90-91 22.086175555270664 25.967744819722178 27.15113048170022 24.79494914330694 92-93 21.927504879054457 26.212245058933405 27.03896882965113 24.821281232361013 94-95 21.488610019871487 25.90377083910593 27.18736530800984 25.420253833012733 96-97 21.371117013699433 25.81167185700142 27.443581883886214 25.373629245412932 98-99 21.36034097865847 25.942539704133843 27.235804372206452 25.461314945001234 100 21.222566430117386 26.01402216739961 27.30273510364872 25.460676298834283 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 885.0 1 653.5 2 1126.5 3 1741.5 4 1294.0 5 1045.0 6 1365.5 7 2451.0 8 3923.5 9 4653.5 10 4590.5 11 4638.0 12 5299.0 13 6166.5 14 7209.0 15 8387.5 16 9566.0 17 10770.0 18 12134.0 19 13585.0 20 15188.5 21 17218.0 22 20030.0 23 24529.0 24 30471.0 25 38677.0 26 50887.5 27 66870.0 28 87312.0 29 115563.5 30 150498.0 31 185076.5 32 228695.5 33 273538.5 34 315274.5 35 359084.0 36 406134.0 37 460493.5 38 491810.5 39 500868.5 40 509168.5 41 518775.5 42 534539.0 43 558394.0 44 588962.5 45 631272.0 46 666976.5 47 694473.5 48 729856.0 49 752467.0 50 761926.5 51 757995.0 52 725529.5 53 680122.0 54 636727.5 55 575692.0 56 508086.0 57 443140.5 58 376890.0 59 315079.0 60 248340.0 61 189277.5 62 145873.0 63 110254.0 64 78504.5 65 53973.0 66 37875.5 67 27294.5 68 19934.0 69 14253.5 70 10373.5 71 7602.0 72 5492.5 73 3931.5 74 2835.5 75 1851.5 76 1180.0 77 730.0 78 433.5 79 271.0 80 170.0 81 115.5 82 82.0 83 53.5 84 36.0 85 27.0 86 18.0 87 11.5 88 10.0 89 8.5 90 5.0 91 3.0 92 2.0 93 1.5 94 0.5 95 0.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05025447106783815 2 0.0 3 1.1858063017422874E-5 4 0.01634633986951743 5 0.004013954331397643 6 1.1858063017422874E-5 7 1.6601288224392025E-4 8 2.549483548745918E-4 9 0.002122593280118694 10-11 8.41922474237024E-4 12-13 3.05345122698639E-4 14-15 4.74322520696915E-5 16-17 1.0672256715680585E-4 18-19 0.0 20-21 2.6680641789201463E-5 22-23 2.9645157543557186E-6 24-25 8.00419253676044E-5 26-27 1.1858063017422874E-5 28-29 8.745321475349369E-4 30-31 9.130708523415612E-4 32-33 0.014469801397010262 34-35 0.023398922849129686 36-37 0.031059231558384866 38-39 0.02076643285926181 40-41 1.6897739799827597E-4 42-43 0.0018142836416656998 44-45 0.00976215037909338 46-47 0.014161491758557269 48-49 0.005466567051031945 50-51 0.010328372888175324 52-53 0.015981704431731678 54-55 0.009874801977758899 56-57 0.002917083502286027 58-59 0.00857930859310545 60-61 0.0016393772121587123 62-63 0.0010257224510070787 64-65 0.01951244269516934 66-67 0.04537784265192298 68-69 0.054876151128878704 70-71 0.04837200356382226 72-73 0.04134313671024485 74-75 0.028821022163846296 76-77 0.029194551148895118 78-79 0.012439108105276596 80-81 0.0020395868389967344 82-83 0.028136219024590122 84-85 0.048054800378106194 86-87 0.04833642937476999 88-89 0.05953933441048025 90-91 0.034936818165082144 92-93 0.026938554659830413 94-95 0.0076721667722726 96-97 0.0040228478786607106 98-99 0.022969068064748105 100 0.01950651366366063 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.6866161E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.62637857216054 #Duplication Level Percentage of deduplicated Percentage of total 1 73.74484764288067 41.75903660372062 2 15.266283114123905 17.289486540203242 3 5.0875071806308645 8.642613227969653 4 2.219972314448653 5.028359707907393 5 1.1372329487936026 3.2198691741560497 6 0.6327210818332506 2.1497222106285965 7 0.4092707822768205 1.6222865579012071 8 0.2847799888496451 1.2900847566700528 9 0.1975914396951871 1.0069998900118105 >10 0.9330612794778408 9.207951708506783 >50 0.05254322993269099 2.03896804045639 >100 0.029566117522643588 3.2652180635261097 >500 0.0029045716594901143 1.1039455697890288 >1k 0.0016127995611655021 1.586381570044227 >5k 6.029046492068915E-5 0.2564193101160589 >10k+ 4.521784869051686E-5 0.5326570683927372 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 27917 0.1655207726286972 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19016 0.1127464631696567 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 5.929031508711437E-6 5.929031508711437E-6 2 0.0 0.0 0.0 5.929031508711437E-6 5.929031508711437E-6 3 0.0 0.0 0.0 5.929031508711437E-6 5.929031508711437E-6 4 0.0 0.0 0.0 1.778709452613431E-5 5.929031508711437E-6 5 0.0 0.0 0.0 1.778709452613431E-5 5.929031508711437E-6 6 0.0 0.0 0.0 1.778709452613431E-5 1.778709452613431E-5 7 0.0 0.0 0.0 1.778709452613431E-5 1.778709452613431E-5 8 0.0 0.0 0.0 1.778709452613431E-5 1.778709452613431E-5 9 0.0 0.0 0.0 2.9645157543557184E-5 2.371612603484575E-5 10-11 0.0 5.929031508711437E-6 0.0 4.74322520696915E-5 2.371612603484575E-5 12-13 0.0 1.1858063017422874E-5 0.0 6.818386235018153E-5 6.225483084147009E-5 14-15 0.0 1.7787094526134313E-5 0.0 8.300644112196011E-5 8.00419253676044E-5 16-17 0.0 2.371612603484575E-5 0.0 9.782901989373872E-5 1.0079353564809442E-4 18-19 0.0 2.371612603484575E-5 0.0 1.3636772470036306E-4 1.1858063017422874E-4 20-21 0.0 2.371612603484575E-5 0.0 3.379547959965519E-4 1.274741774372959E-4 22-23 0.0 2.371612603484575E-5 0.0 0.0012777062901273145 1.6304836648956453E-4 24-25 0.0 2.371612603484575E-5 0.0 0.0030564157427407455 1.89729008278766E-4 26-27 0.0 2.371612603484575E-5 0.0 0.005929031508711437 1.9565803978747743E-4 28-29 8.893547263067157E-6 2.371612603484575E-5 0.0 0.01505974003212705 2.0158707129618888E-4 30-31 1.1858063017422874E-5 2.371612603484575E-5 0.0 0.053456148082542315 2.075161028049003E-4 32-33 1.1858063017422874E-5 2.371612603484575E-5 0.0 0.14193212077128875 2.1344513431361173E-4 34-35 1.1858063017422874E-5 2.371612603484575E-5 0.0 0.2561904869756668 2.1344513431361173E-4 36-37 1.1858063017422874E-5 3.853870480662434E-5 0.0 0.42843181681948844 2.1344513431361173E-4 38-39 1.1858063017422874E-5 5.632579933275865E-5 0.0 0.7511519663544064 2.223386815766789E-4 40-41 1.1858063017422874E-5 6.521934659582581E-5 0.0 1.1599646179115686 2.3419674459410175E-4 42-43 1.1858063017422874E-5 7.114837810453724E-5 0.0 1.5511235781515427 2.579128706289475E-4 44-45 1.1858063017422874E-5 7.114837810453724E-5 0.0 1.9690461866218403 2.6384190213765897E-4 46-47 1.1858063017422874E-5 7.114837810453724E-5 0.0 2.4225311260813887 2.8459351241814896E-4 48-49 1.1858063017422874E-5 7.707740961324868E-5 0.0 2.920193279312346 3.023806069442833E-4 50-51 1.1858063017422874E-5 8.300644112196012E-5 0.0 3.4702443549542776 3.201677014704176E-4 52-53 1.1858063017422874E-5 9.4864504139383E-5 0.0 4.041127082802067 3.5870640627704193E-4 54-55 1.1858063017422874E-5 1.1858063017422874E-4 0.0 4.6334699401956385 3.9131607957495487E-4 56-57 1.1858063017422874E-5 1.3043869319165162E-4 0.0 5.23334859663678 3.9131607957495487E-4 58-59 1.1858063017422874E-5 1.3636772470036306E-4 0.0 5.926894092852546 3.9131607957495487E-4 60-61 1.1858063017422874E-5 1.5119030347214164E-4 0.0 6.62528360781093 4.031741425923777E-4 62-63 1.1858063017422874E-5 1.5415481922649736E-4 0.0 7.376391106429021 4.2689026862722347E-4 64-65 1.1858063017422874E-5 1.5415481922649736E-4 0.0 8.050441947044144 4.2689026862722347E-4 66-67 1.1858063017422874E-5 1.5415481922649736E-4 0.0 8.772378610639375 4.328193001359349E-4 68-69 1.1858063017422874E-5 1.5415481922649736E-4 0.0 9.586870420601345 4.3874833164464635E-4 70-71 1.1858063017422874E-5 1.630483664895645E-4 0.0 10.427455898233156 4.3874833164464635E-4 72-73 1.1858063017422874E-5 1.7490642950698739E-4 0.0 11.20162436490438 5.098967097491836E-4 74-75 1.1858063017422874E-5 1.778709452613431E-4 0.0 11.936026817246674 5.098967097491836E-4 76-77 1.1858063017422874E-5 1.778709452613431E-4 0.0 12.705048884568338 5.098967097491836E-4 78-79 1.1858063017422874E-5 1.8379997677005455E-4 0.0 13.52520588413688 5.098967097491836E-4 80-81 1.1858063017422874E-5 1.8379997677005455E-4 0.0 14.330350575925369 5.187902570122508E-4 82-83 1.778709452613431E-5 1.8379997677005455E-4 0.0 15.161832025675551 5.365773515383851E-4 84-85 1.778709452613431E-5 1.8379997677005455E-4 0.0 15.984390283005125 5.395418672927408E-4 86-87 1.778709452613431E-5 1.89729008278766E-4 0.0 16.789179232903088 5.425063830470965E-4 88 1.778709452613431E-5 1.89729008278766E-4 0.0 17.3864165058071 5.513999303101637E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 18510 0.0 50.97812 2 ACCTGGG 21715 0.0 42.199413 3 GGTATCA 20085 0.0 41.43243 1 GTATCAA 36315 0.0 40.457867 1 CCTGGGG 20060 0.0 32.562717 4 GTACCTG 29955 0.0 32.002724 1 CAACGCA 44980 0.0 31.902489 5 TGGGGGG 34385 0.0 31.770004 6 ATCAACG 45215 0.0 31.647282 3 GTACATG 58545 0.0 30.805466 1 TCAACGC 47040 0.0 30.52972 4 TATCAAC 47490 0.0 30.487091 2 AACGCAG 48905 0.0 29.879307 6 TACATGG 60220 0.0 29.442356 2 ACATGGG 59610 0.0 28.364405 3 ATGGGGG 34745 0.0 28.290743 5 CATGGGG 45145 0.0 28.18875 4 CTGGGGG 13825 0.0 27.52928 5 ACGCAGA 54565 0.0 26.39245 7 CGCAGAG 56305 0.0 25.476704 8 >>END_MODULE