FastQCFastQC Report
Mon 6 Jun 2016
ERR1211420_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211420_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16179946
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT271580.1678497567297196No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT203930.12603873956068828No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG134850.035.3203432
GTACATG566300.028.3015061
GTATCAA560100.027.538161
TACATGG570600.027.3018192
ACCTGGG172300.027.2009373
GTACTAG72300.026.650591
ACATGGG564600.026.4681533
GGTATCA391650.025.1643311
CATGGGG422800.024.2598654
CAACGCA621600.024.101335
ATCAACG629900.023.746663
TATCAAC657700.023.0636392
AACGCAG660200.023.0417986
TCAACGC657850.022.9018084
GTACCTG216950.022.800871
ATGGGGG311400.022.4623015
GCATCGT75100.021.5947288
TGGGGGG325500.021.57666
ACGCAGA712200.020.9176587
CGCAGAG720700.020.532838
Produced by FastQC (version 0.11.5)