##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211420_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 16179946 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.208273068402082 31.0 31.0 34.0 28.0 34.0 2 31.265825485449703 31.0 31.0 34.0 28.0 34.0 3 31.340209540872387 31.0 31.0 34.0 28.0 34.0 4 34.955810050293124 37.0 35.0 37.0 32.0 37.0 5 35.00851010256771 37.0 35.0 37.0 32.0 37.0 6 35.064556395923695 37.0 35.0 37.0 32.0 37.0 7 34.9849652155823 37.0 35.0 37.0 32.0 37.0 8 34.98260358841741 37.0 35.0 37.0 32.0 37.0 9 36.532332617179314 39.0 37.0 39.0 32.0 39.0 10-11 36.45348624772913 39.0 37.0 39.0 32.0 39.0 12-13 36.36195364928906 39.0 37.0 39.0 32.0 39.0 14-15 37.528380286312455 40.0 37.0 41.0 32.0 41.0 16-17 37.52612582885011 40.0 37.0 41.0 32.0 41.0 18-19 37.509968760093514 40.0 37.0 41.0 31.5 41.0 20-21 37.49042632775165 40.0 37.0 41.0 31.0 41.0 22-23 37.36773086263699 40.0 37.0 41.0 31.0 41.0 24-25 37.27296141779459 40.0 36.5 41.0 31.0 41.0 26-27 37.13496129715142 40.0 36.0 41.0 30.5 41.0 28-29 37.01764449646494 39.5 36.0 41.0 30.0 41.0 30-31 36.88093001052043 39.0 36.0 41.0 30.0 41.0 32-33 36.71676534025515 39.0 36.0 41.0 30.0 41.0 34-35 36.604406281702055 39.0 35.0 41.0 30.0 41.0 36-37 36.48502216880081 39.0 35.0 41.0 29.5 41.0 38-39 36.339387257534725 39.0 35.0 40.0 29.0 41.0 40-41 36.188122043176165 39.0 35.0 40.0 28.5 41.0 42-43 36.008607847022475 38.5 35.0 40.0 28.0 41.0 44-45 35.88674106205299 38.0 35.0 40.0 27.0 41.0 46-47 35.69724280909219 38.0 34.0 40.0 27.0 41.0 48-49 35.48241023177704 38.0 34.0 40.0 26.0 41.0 50-51 34.84153389634304 37.5 33.5 39.5 25.5 40.5 52-53 35.15981073113594 38.0 34.0 39.5 26.5 40.5 54-55 35.58025910593274 38.0 34.0 40.0 27.0 41.0 56-57 35.5033643499181 38.0 34.0 40.0 27.0 41.0 58-59 35.29938094354579 38.0 34.0 40.0 26.0 41.0 60-61 35.07555578368432 38.0 34.0 40.0 26.0 41.0 62-63 34.80655701817546 37.0 33.5 40.0 26.0 41.0 64-65 34.60711568505853 37.0 33.0 40.0 26.0 41.0 66-67 34.4172886300115 36.0 33.0 39.5 26.0 41.0 68-69 34.095660949671895 36.0 33.0 39.0 25.5 41.0 70-71 33.743471486246 35.0 32.5 39.0 25.0 41.0 72-73 33.3855785118195 35.0 32.0 38.5 24.5 40.0 74-75 33.03352999447588 35.0 32.0 37.0 24.0 40.0 76-77 32.68089698816053 35.0 32.0 37.0 23.5 39.0 78-79 32.32017109327806 35.0 31.0 36.0 23.0 39.0 80-81 31.960518131519102 35.0 31.0 36.0 22.0 38.5 82-83 31.631083873827514 35.0 31.0 35.5 20.5 37.0 84-85 31.332239056916507 34.0 30.5 35.0 20.0 37.0 86-87 31.046708684936277 34.0 30.0 35.0 20.0 36.5 88-89 30.779312736890468 34.0 30.0 35.0 19.0 36.0 90-91 30.518783653542478 34.0 29.5 35.0 18.5 36.0 92-93 30.274674773327426 34.0 29.0 35.0 18.0 35.5 94-95 30.045457382861475 34.0 29.0 35.0 17.5 35.0 96-97 29.80571953083156 34.0 29.0 35.0 16.5 35.0 98-99 29.54654462382013 34.0 28.5 35.0 15.0 35.0 100 28.314474782548718 33.0 25.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.377784720575459 1101 2 -0.3469090693735062 1101 3 -0.4424658177622227 1101 4 -0.3671471997882918 1101 5 -0.307482521618347 1101 6 -0.36346313955591825 1101 7 -0.3500600833387608 1101 8 -0.34323463493890216 1101 9 -0.534915160750792 1101 10-11 -0.47267663980188246 1101 12-13 -0.5215513033745012 1101 14-15 -0.760491147287361 1101 16-17 -0.6990782299589213 1101 18-19 -0.7188084557165979 1101 20-21 -0.6891706129553583 1101 22-23 -0.6732960589868213 1101 24-25 -0.7698527451653447 1101 26-27 -0.812797963822895 1101 28-29 -0.8643839273016525 1101 30-31 -0.884850250552681 1101 32-33 -0.8953283212054188 1101 34-35 -0.9281405791378461 1101 36-37 -0.9060096856463105 1101 38-39 -0.9359604742969978 1101 40-41 -1.0814872763352739 1101 42-43 -1.0447935806735202 1101 44-45 -1.0806603177027725 1101 46-47 -1.1107028440264273 1101 48-49 -1.175827358471885 1101 50-51 -1.0522605889670231 1101 52-53 -0.8513311297876243 1101 54-55 -0.8459571277309053 1101 56-57 -0.8766355362254572 1101 58-59 -0.8689584600597939 1101 60-61 -0.9172788928962916 1101 62-63 -1.0232880982816752 1101 64-65 -0.9351443583384977 1101 66-67 -0.9923489094809455 1101 68-69 -1.0211676416357207 1101 70-71 -1.022140788050173 1101 72-73 -1.0807646669773945 1101 74-75 -1.1875007361964691 1101 76-77 -1.1599238848001008 1101 78-79 -1.1444584241090823 1101 80-81 -1.19235539609328 1101 82-83 -1.1759157621697298 1101 84-85 -1.268689614162028 1101 86-87 -1.3646060718202477 1101 88-89 -1.4512804884304416 1101 90-91 -1.5874062609506758 1101 92-93 -1.606586042632241 1101 94-95 -1.7242750245550589 1101 96-97 -1.8449626931725724 1101 98-99 -1.9166036616474607 1101 100 -1.9143812791918435 1102 1 -0.38815926906874765 1102 2 -0.4213355333233082 1102 3 -0.43494606949121106 1102 4 -0.3726353097292119 1102 5 -0.3076669120208493 1102 6 -0.289768364996867 1102 7 -0.37038281008636176 1102 8 -0.3029364670264485 1102 9 -0.4517139429105228 1102 10-11 -0.4454829725118472 1102 12-13 -0.5302114493072168 1102 14-15 -0.6678103076178346 1102 16-17 -0.6660936936100299 1102 18-19 -0.7378048719727843 1102 20-21 -0.7153759848424883 1102 22-23 -0.6647360375637348 1102 24-25 -0.7060849753417315 1102 26-27 -0.7784695071214429 1102 28-29 -0.8178856564595165 1102 30-31 -0.7336448984888264 1102 32-33 -0.8326531103933092 1102 34-35 -0.9110995235306802 1102 36-37 -0.9448340148867445 1102 38-39 -1.014499306387897 1102 40-41 -1.1274479423350101 1102 42-43 -1.0982296814380206 1102 44-45 -1.0683169989672407 1102 46-47 -1.2570581553288847 1102 48-49 -1.2609980941749157 1102 50-51 -1.0686086048979746 1102 52-53 -0.9053706441230105 1102 54-55 -0.9493053769692992 1102 56-57 -0.9252466392499343 1102 58-59 -0.9272059790573621 1102 60-61 -0.9782550251026407 1102 62-63 -0.906016495541401 1102 64-65 -1.004602927083745 1102 66-67 -1.0555988433607126 1102 68-69 -1.0902520920186802 1102 70-71 -1.0730320815051044 1102 72-73 -1.0334611001076084 1102 74-75 -1.1985950846489857 1102 76-77 -1.1776097721540744 1102 78-79 -1.2344205803294415 1102 80-81 -1.2558986396209129 1102 82-83 -1.3024389035820967 1102 84-85 -1.3544485686155703 1102 86-87 -1.3872038938512858 1102 88-89 -1.449757125563817 1102 90-91 -1.5102392581122963 1102 92-93 -1.5881329675066702 1102 94-95 -1.6443313636956916 1102 96-97 -1.7845912341643775 1102 98-99 -1.8836916059736772 1102 100 -1.8639743577339054 1103 1 -0.4062057312510703 1103 2 -0.4612158925453578 1103 3 -0.523861600722423 1103 4 -0.3624384741746596 1103 5 -0.308338078348136 1103 6 -0.35142531665919563 1103 7 -0.4080380488320614 1103 8 -0.4249277643927698 1103 9 -0.5806472118202635 1103 10-11 -0.547731400370445 1103 12-13 -0.5583077777076682 1103 14-15 -0.6544856259060765 1103 16-17 -0.7461502602748169 1103 18-19 -0.7545041470257843 1103 20-21 -0.7139414398734303 1103 22-23 -0.7410010096902369 1103 24-25 -0.8074840926356472 1103 26-27 -0.8391508024533394 1103 28-29 -0.8428606847507965 1103 30-31 -0.7783834266702101 1103 32-33 -0.8722010697882823 1103 34-35 -0.8555991243648009 1103 36-37 -0.9356255531149387 1103 38-39 -0.9054954091846952 1103 40-41 -0.9374647899508233 1103 42-43 -1.0263021007556503 1103 44-45 -0.9961004703208474 1103 46-47 -1.1043750203826548 1103 48-49 -1.0557988223007087 1103 50-51 -1.0540812554502637 1103 52-53 -0.848899020775761 1103 54-55 -0.843416420013817 1103 56-57 -0.8572393965915879 1103 58-59 -0.8295071681084991 1103 60-61 -0.8266961846908814 1103 62-63 -0.8189420022868106 1103 64-65 -0.8220272263409996 1103 66-67 -0.9210333097681129 1103 68-69 -0.8988123996715771 1103 70-71 -0.9693543877484458 1103 72-73 -0.8985634073681581 1103 74-75 -0.9627072538953207 1103 76-77 -1.000988963395855 1103 78-79 -0.9839053271065978 1103 80-81 -0.9753046067648832 1103 82-83 -0.9871316139684048 1103 84-85 -1.0259483583609565 1103 86-87 -1.1662399014295346 1103 88-89 -1.1957362490518442 1103 90-91 -1.1580966818711609 1103 92-93 -1.2336543285578863 1103 94-95 -1.234783833438101 1103 96-97 -1.3952378238618124 1103 98-99 -1.491309228216526 1103 100 -1.4970430760254203 1104 1 -0.545650295307162 1104 2 -0.5330209343714003 1104 3 -0.5644708664110176 1104 4 -0.34028591047169954 1104 5 -0.4258498703547957 1104 6 -0.3731106637621835 1104 7 -0.36408255577484283 1104 8 -0.4853205219927119 1104 9 -0.49202272372307476 1104 10-11 -0.5069174749024157 1104 12-13 -0.5910996005951432 1104 14-15 -0.8105143343525398 1104 16-17 -0.7997272853199959 1104 18-19 -0.8400058973595463 1104 20-21 -0.8280306807708868 1104 22-23 -0.8422583515431228 1104 24-25 -0.8129143544920652 1104 26-27 -0.8709957042346588 1104 28-29 -1.021064169383358 1104 30-31 -1.0049555988857648 1104 32-33 -1.0541866153465094 1104 34-35 -0.95372285980946 1104 36-37 -1.0686859256666779 1104 38-39 -1.1460309905963797 1104 40-41 -1.0985942371768616 1104 42-43 -1.1302802364475113 1104 44-45 -1.1565201180200049 1104 46-47 -1.093679737905994 1104 48-49 -1.1403063979217976 1104 50-51 -1.079910452422716 1104 52-53 -0.90079893465537 1104 54-55 -0.8956461700124692 1104 56-57 -0.844562041298154 1104 58-59 -0.8685174638185131 1104 60-61 -0.8870987749686492 1104 62-63 -0.9440027437328737 1104 64-65 -1.0015640455080117 1104 66-67 -0.8718106971314725 1104 68-69 -0.9440517193025286 1104 70-71 -0.9226163234392146 1104 72-73 -0.9190748642620221 1104 74-75 -0.9241809461897077 1104 76-77 -0.9911306701649565 1104 78-79 -0.8954835484684196 1104 80-81 -0.965820160232493 1104 82-83 -1.073494117729414 1104 84-85 -1.0573185710674977 1104 86-87 -1.1257758242599074 1104 88-89 -1.1516614666499514 1104 90-91 -1.1544580280516819 1104 92-93 -1.17891943025381 1104 94-95 -1.2572352465871326 1104 96-97 -1.3045409739220801 1104 98-99 -1.386624002276637 1104 100 -1.4217347452294575 1105 1 -0.5214025323861797 1105 2 -0.5413991550539023 1105 3 -0.5591368577276192 1105 4 -0.420670268556151 1105 5 -0.4952640387879015 1105 6 -0.44238011495653495 1105 7 -0.4586552469686751 1105 8 -0.48236742579705805 1105 9 -0.627760573937941 1105 10-11 -0.5826537987926272 1105 12-13 -0.6462478167046442 1105 14-15 -0.8010208653500754 1105 16-17 -0.7866872020250923 1105 18-19 -0.7812290012355376 1105 20-21 -0.8359366181671462 1105 22-23 -0.796448432373424 1105 24-25 -0.7444382178134603 1105 26-27 -0.8340683083145493 1105 28-29 -0.823994220722561 1105 30-31 -0.868763326630571 1105 32-33 -0.8599264437485559 1105 34-35 -0.9095892320639223 1105 36-37 -0.9019064533820327 1105 38-39 -1.0873199846395565 1105 40-41 -1.0054726162545933 1105 42-43 -0.9145906954829925 1105 44-45 -0.9763226490934969 1105 46-47 -1.0511647783825069 1105 48-49 -1.0212507832714408 1105 50-51 -1.0850729519984768 1105 52-53 -0.8780489214895084 1105 54-55 -0.7959531635503438 1105 56-57 -0.8720554098405202 1105 58-59 -0.8290794434767221 1105 60-61 -0.8426798495464212 1105 62-63 -0.9159863685319962 1105 64-65 -0.8477433385174251 1105 66-67 -0.8941706409500938 1105 68-69 -0.8874581580768321 1105 70-71 -0.8352761803182105 1105 72-73 -0.8805687788709449 1105 74-75 -0.8869317381359849 1105 76-77 -0.9248675803482413 1105 78-79 -0.975612573029764 1105 80-81 -0.9800004675058283 1105 82-83 -1.0165996208350307 1105 84-85 -0.9357676094000098 1105 86-87 -0.9406627586133922 1105 88-89 -1.0445322664803065 1105 90-91 -1.0587281630569976 1105 92-93 -1.1061779908841807 1105 94-95 -1.161845579261712 1105 96-97 -1.1777090873533531 1105 98-99 -1.1683120547803973 1105 100 -1.2291101673418652 1106 1 -0.4995617699531216 1106 2 -0.5193356261325519 1106 3 -0.5628268269149643 1106 4 -0.34275222939904637 1106 5 -0.41735223949108047 1106 6 -0.40083197485595434 1106 7 -0.4867578482438901 1106 8 -0.45146625587626943 1106 9 -0.619380441580077 1106 10-11 -0.6586305657070497 1106 12-13 -0.6870054586280787 1106 14-15 -0.7616316017562212 1106 16-17 -0.7539759816030625 1106 18-19 -0.7986765261615005 1106 20-21 -0.8752657535808837 1106 22-23 -0.8194363166418128 1106 24-25 -0.8659622386813979 1106 26-27 -0.8546829512382388 1106 28-29 -0.8831172857933751 1106 30-31 -0.9387548182595253 1106 32-33 -1.0164917253449843 1106 34-35 -0.9587641521987109 1106 36-37 -0.9019131147370558 1106 38-39 -1.0075128190916516 1106 40-41 -0.9860477378325783 1106 42-43 -0.8853608173876992 1106 44-45 -0.9773796623058146 1106 46-47 -0.9568455054677187 1106 48-49 -0.9917003410448118 1106 50-51 -0.9391417893375618 1106 52-53 -0.8782264859258646 1106 54-55 -0.7839120821352807 1106 56-57 -0.851005180347066 1106 58-59 -0.8557585527260656 1106 60-61 -0.7628140267046888 1106 62-63 -0.8280416690938495 1106 64-65 -0.8137951396648262 1106 66-67 -0.7951464069231449 1106 68-69 -0.7920680690832995 1106 70-71 -0.8516198344181021 1106 72-73 -0.7608035947206275 1106 74-75 -0.8341653004255463 1106 76-77 -0.8611530383651562 1106 78-79 -0.819737376092867 1106 80-81 -0.8165218183132161 1106 82-83 -0.8396419565749902 1106 84-85 -0.8571666227494816 1106 86-87 -0.8780926878286088 1106 88-89 -0.9189560820837777 1106 90-91 -0.9010701237561527 1106 92-93 -0.8774428589699284 1106 94-95 -0.9215028472011078 1106 96-97 -0.9920124965966117 1106 98-99 -1.0402187733187844 1106 100 -1.0318357461343162 1107 1 -0.5270142946286853 1107 2 -0.5330749885602621 1107 3 -0.5575438951545699 1107 4 -0.40593734213423716 1107 5 -0.3883677768854241 1107 6 -0.4641122479008857 1107 7 -0.4658206953182713 1107 8 -0.4789834744502315 1107 9 -0.6109914852903913 1107 10-11 -0.580004205903073 1107 12-13 -0.6424450612662653 1107 14-15 -0.804562895112646 1107 16-17 -0.8836157492514403 1107 18-19 -0.8434544949617333 1107 20-21 -0.865743066436778 1107 22-23 -0.8215189422795035 1107 24-25 -0.8885667749581145 1107 26-27 -0.8742489118932539 1107 28-29 -0.890779872660751 1107 30-31 -0.8942337296575076 1107 32-33 -0.9611247004311778 1107 34-35 -1.00622443869878 1107 36-37 -0.9735706193638407 1107 38-39 -0.964505894577691 1107 40-41 -0.9355459551463596 1107 42-43 -0.9522224750299486 1107 44-45 -0.9723167916801359 1107 46-47 -0.9262313169632321 1107 48-49 -1.0006269549947788 1107 50-51 -1.043008252862002 1107 52-53 -0.8710825751724087 1107 54-55 -0.8338548951063061 1107 56-57 -0.808202894859356 1107 58-59 -0.88808609375657 1107 60-61 -0.846056472327291 1107 62-63 -0.7664699856777091 1107 64-65 -0.7768836666171026 1107 66-67 -0.8314338194491597 1107 68-69 -0.7986152805041726 1107 70-71 -0.8084562819009093 1107 72-73 -0.7473093177407009 1107 74-75 -0.7101630478043788 1107 76-77 -0.8261106183127858 1107 78-79 -0.8124270483512817 1107 80-81 -0.7821681165983918 1107 82-83 -0.8052487380950595 1107 84-85 -0.8603996451369369 1107 86-87 -0.825279524596759 1107 88-89 -0.8470290619970591 1107 90-91 -0.9366784901918308 1107 92-93 -0.9713231379527691 1107 94-95 -0.9454816066830425 1107 96-97 -0.9210101917363964 1107 98-99 -0.9429898591976347 1107 100 -0.9698733523238943 1108 1 -0.5437158889540115 1108 2 -0.5456127230615877 1108 3 -0.5747399571903102 1108 4 -0.4576275310244924 1108 5 -0.4216213695841162 1108 6 -0.4705872316710398 1108 7 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0.668814835918937 2311 6 0.6533710293851627 2311 7 0.7293800218267208 2311 8 0.7000608491811064 2311 9 0.8909236000204714 2311 10-11 0.7885378607134399 2311 12-13 0.9574351722921932 2311 14-15 1.1712185337155958 2311 16-17 1.1694239138516735 2311 18-19 1.171636154914772 2311 20-21 1.2357751868835223 2311 22-23 1.2604348851598317 2311 24-25 1.2550924045506449 2311 26-27 1.2904244631107318 2311 28-29 1.3195631291869816 2311 30-31 1.3822728341476065 2311 32-33 1.3890339318853435 2311 34-35 1.3911357857596727 2311 36-37 1.4231056328185332 2311 38-39 1.4740547053947992 2311 40-41 1.543843204293026 2311 42-43 1.548512905581262 2311 44-45 1.5660471584130065 2311 46-47 1.595257331085996 2311 48-49 1.6382773215630593 2311 50-51 1.5814593566130455 2311 52-53 1.4775666740394087 2311 54-55 1.406247118961808 2311 56-57 1.3958971883102365 2311 58-59 1.3461473296728599 2311 60-61 1.3267212951897562 2311 62-63 1.344369040973028 2311 64-65 1.2966987455820131 2311 66-67 1.3103370058476003 2311 68-69 1.3695694582899094 2311 70-71 1.3410385440846326 2311 72-73 1.3479390644590197 2311 74-75 1.3350441665470356 2311 76-77 1.3326418260153403 2311 78-79 1.3158998770337078 2311 80-81 1.3363266597032357 2311 82-83 1.361749068873852 2311 84-85 1.3455744152065137 2311 86-87 1.4208328429566421 2311 88-89 1.4844048653874573 2311 90-91 1.5207084371570474 2311 92-93 1.6058635971957465 2311 94-95 1.6399593139646207 2311 96-97 1.6624568897466823 2311 98-99 1.7289198914239172 2311 100 1.8379217490727804 2312 1 0.5645721613065611 2312 2 0.6129499817423785 2312 3 0.6628742779614356 2312 4 0.5055903035767457 2312 5 0.5087363263845077 2312 6 0.3789514577663198 2312 7 0.5157499976460542 2312 8 0.5806183099255833 2312 9 0.7597256440163918 2312 10-11 0.7764735632456663 2312 12-13 0.860667286583876 2312 14-15 1.0185613385218417 2312 16-17 1.0407189645468478 2312 18-19 1.021640066875797 2312 20-21 1.0425670693633435 2312 22-23 1.0767448413809149 2312 24-25 1.068048619505376 2312 26-27 1.1155909060092668 2312 28-29 1.1586201169728199 2312 30-31 1.0848590634033926 2312 32-33 1.184986684340629 2312 34-35 1.2142257593064798 2312 36-37 1.223554843828282 2312 38-39 1.2695997219335382 2312 40-41 1.3113015357471127 2312 42-43 1.3482719633827926 2312 44-45 1.3532422601350191 2312 46-47 1.2983378404540673 2312 48-49 1.3650997652516565 2312 50-51 1.2717151661337098 2312 52-53 1.1686763172552048 2312 54-55 1.1359459156758192 2312 56-57 1.112414017215606 2312 58-59 1.1275157823220496 2312 60-61 1.1236172076132362 2312 62-63 1.1258019393500902 2312 64-65 1.104318285751141 2312 66-67 1.0985002429734223 2312 68-69 1.1170991936631651 2312 70-71 1.1660507859124678 2312 72-73 1.1492074879100116 2312 74-75 1.1555307276943338 2312 76-77 1.1162159626495551 2312 78-79 1.1344686077465056 2312 80-81 1.0135803036483075 2312 82-83 1.145549761217513 2312 84-85 1.2009887992014256 2312 86-87 1.2640149472974826 2312 88-89 1.2970555207879428 2312 90-91 1.3133618934479117 2312 92-93 1.3783866680979706 2312 94-95 1.4199552082070888 2312 96-97 1.4809798846485478 2312 98-99 1.3460647291087788 2312 100 1.4782812328492696 2313 1 0.6140775552897111 2313 2 0.624307943954495 2313 3 0.6385237182818173 2313 4 0.5773184619497016 2313 5 0.552709023306889 2313 6 0.5774049295661214 2313 7 0.666079587227614 2313 8 0.6609535884406128 2313 9 0.7951193388230351 2313 10-11 0.6665886146299513 2313 12-13 0.7863691944010185 2313 14-15 1.0145703378242743 2313 16-17 1.0190026575304998 2313 18-19 0.9945207511363563 2313 20-21 1.0251753097363263 2313 22-23 1.0529256293071896 2313 24-25 1.1353854894586206 2313 26-27 1.1499137868502558 2313 28-29 1.163394178604328 2313 30-31 1.166301989220237 2313 32-33 1.221901307905867 2313 34-35 1.2230285351580932 2313 36-37 1.2263875885051334 2313 38-39 1.2697908275722298 2313 40-41 1.2423356964885741 2313 42-43 1.2911536044498817 2313 44-45 1.2799768502734636 2313 46-47 1.2901940299109071 2313 48-49 1.3500069658709961 2313 50-51 1.3084641854962413 2313 52-53 1.141640768359963 2313 54-55 1.1176924682394755 2313 56-57 1.1133801089771183 2313 58-59 1.121363010608114 2313 60-61 1.1164512215427962 2313 62-63 1.154455111002882 2313 64-65 1.12142937778016 2313 66-67 1.1120450555212855 2313 68-69 1.1073116170961725 2313 70-71 1.0912595550007467 2313 72-73 1.1262014029246288 2313 74-75 1.1159616671012174 2313 76-77 1.139228822806274 2313 78-79 1.1319987462000398 2313 80-81 1.1601549396082227 2313 82-83 1.176173923157922 2313 84-85 1.2096180225410365 2313 86-87 1.2691549081291669 2313 88-89 1.2849214898637236 2313 90-91 1.3594167810870097 2313 92-93 1.4317198208310877 2313 94-95 1.5091585348513696 2313 96-97 1.5685412056417327 2313 98-99 1.552375624639712 2313 100 1.6539883638601154 2314 1 0.550180702254913 2314 2 0.5690552491147436 2314 3 0.5795307896044513 2314 4 0.47850515387433745 2314 5 0.514012565434669 2314 6 0.5394635009693687 2314 7 0.5822764531860756 2314 8 0.5538608294628844 2314 9 0.7367881833375947 2314 10-11 0.7185610363143695 2314 12-13 0.5132404728901108 2314 14-15 0.8963904258614477 2314 16-17 0.9033390617141848 2314 18-19 0.932739437802276 2314 20-21 0.9861362880995799 2314 22-23 0.8410847054419719 2314 24-25 0.8720123747499784 2314 26-27 1.001902089166684 2314 28-29 1.044283753342178 2314 30-31 0.9977615185716431 2314 32-33 1.0339888217411115 2314 34-35 1.090686393484745 2314 36-37 1.1814738341237288 2314 38-39 1.222885008245889 2314 40-41 1.2624098344801595 2314 42-43 1.3541581150701774 2314 44-45 1.375327842180461 2314 46-47 1.3209438560124411 2314 48-49 1.3669932065736603 2314 50-51 1.2662003777434094 2314 52-53 1.136600477303702 2314 54-55 1.1163388327240895 2314 56-57 1.0992405806128502 2314 58-59 1.1214124792923172 2314 60-61 1.1182885659870578 2314 62-63 1.1637320926877166 2314 64-65 1.0986807081862509 2314 66-67 1.1199107626896918 2314 68-69 1.1109491793565027 2314 70-71 1.1019387896177335 2314 72-73 1.130999381497567 2314 74-75 1.1556394843182645 2314 76-77 1.1451134667361131 2314 78-79 1.188504545043081 2314 80-81 1.2487223557792966 2314 82-83 1.0951161186752536 2314 84-85 1.0999548284859344 2314 86-87 1.288222102488234 2314 88-89 1.410203806298835 2314 90-91 1.4447701035368965 2314 92-93 1.554510236897432 2314 94-95 1.5889353629313412 2314 96-97 1.5989676254616398 2314 98-99 1.6558565153578435 2314 100 1.5691356355724295 2315 1 0.47103506952745633 2315 2 0.5032909127326626 2315 3 0.5499027062162547 2315 4 0.4297301871031891 2315 5 0.41276403089167957 2315 6 -0.3094627340705429 2315 7 0.2279840686632042 2315 8 0.3618282406261031 2315 9 0.5648309891479713 2315 10-11 0.6492342335906116 2315 12-13 0.689998830204992 2315 14-15 0.8001546641007309 2315 16-17 0.7198257586033705 2315 18-19 0.8053711054898045 2315 20-21 0.8506027746151545 2315 22-23 0.7463357640968056 2315 24-25 0.8823750351023847 2315 26-27 0.9311438207072982 2315 28-29 0.9927174495177908 2315 30-31 1.0697930725001399 2315 32-33 1.0864331762459543 2315 34-35 1.0674620718825238 2315 36-37 1.1243264723402717 2315 38-39 1.1882235269558592 2315 40-41 1.2180956511416312 2315 42-43 1.2954745497190316 2315 44-45 1.27765217784669 2315 46-47 1.2901702123690555 2315 48-49 1.2597580001989357 2315 50-51 1.177303199703779 2315 52-53 1.1176174600521875 2315 54-55 0.9383934196883317 2315 56-57 0.9065759294302964 2315 58-59 0.900516552638642 2315 60-61 0.7980710977630068 2315 62-63 1.000771513823615 2315 64-65 1.0465765298881777 2315 66-67 1.0341365932440354 2315 68-69 1.0708244323409701 2315 70-71 1.0792656538568366 2315 72-73 1.105312901680854 2315 74-75 1.0939615734482615 2315 76-77 1.0657118435605852 2315 78-79 1.0010721105059943 2315 80-81 1.036920786804064 2315 82-83 1.1845489302351488 2315 84-85 1.1103193006452727 2315 86-87 1.210428532960151 2315 88-89 1.3269491363436643 2315 90-91 1.410952116851707 2315 92-93 1.429798789650846 2315 94-95 1.5474110361558822 2315 96-97 1.5914573792304267 2315 98-99 1.610417626457508 2315 100 1.6772813029707478 2316 1 0.09745181776361989 2316 2 0.011318580703399306 2316 3 0.1346323570572494 2316 4 0.10183257331067352 2316 5 0.10829645507210728 2316 6 0.19755778518104705 2316 7 0.21225854646453257 2316 8 0.2530101085451051 2316 9 0.3930743947584858 2316 10-11 0.218774955243056 2316 12-13 0.2786111913541589 2316 14-15 0.5294927750366085 2316 16-17 0.5771514621340472 2316 18-19 0.5115909740693354 2316 20-21 0.4712989953670004 2316 22-23 0.31432397902761267 2316 24-25 0.39397863864851246 2316 26-27 0.49224486269232415 2316 28-29 0.6350101220485769 2316 30-31 0.6122179748240413 2316 32-33 0.7089244504443784 2316 34-35 0.7216474659174992 2316 36-37 0.7637792505755812 2316 38-39 0.8481523619630735 2316 40-41 0.8114749382746211 2316 42-43 0.8430486739522607 2316 44-45 0.6868830326626565 2316 46-47 0.7163677214315172 2316 48-49 0.8271278311616541 2316 50-51 0.614737879083151 2316 52-53 0.36863206782675917 2316 54-55 0.28224515296918895 2316 56-57 0.4251165052286012 2316 58-59 0.444077924405164 2316 60-61 0.500362056295927 2316 62-63 0.5509704356613483 2316 64-65 0.5640093529098635 2316 66-67 0.5832215039509876 2316 68-69 0.5749012467206356 2316 70-71 0.43822446197327025 2316 72-73 0.5426273613226087 2316 74-75 0.6657537976014467 2316 76-77 0.7294544252618138 2316 78-79 0.7850670566872111 2316 80-81 0.854768915272917 2316 82-83 0.8567727635073972 2316 84-85 0.8875227217172608 2316 86-87 1.0153696858943349 2316 88-89 1.0302209790482415 2316 90-91 1.0604342563026385 2316 92-93 1.1303320952499654 2316 94-95 1.2297784189812937 2316 96-97 1.2670969552586797 2316 98-99 1.3831279803278242 2316 100 1.242689662278746 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 70.0 1 931.0 2 472.0 3 327.0 4 272.0 5 239.0 6 329.0 7 1811.0 8 5986.0 9 10014.0 10 14418.0 11 19711.0 12 25808.0 13 31965.0 14 37551.0 15 44868.0 16 54340.0 17 66801.0 18 81069.0 19 95985.0 20 114630.0 21 135008.0 22 148569.0 23 155313.0 24 164104.0 25 179457.0 26 204921.0 27 239641.0 28 283700.0 29 335752.0 30 403941.0 31 496655.0 32 621299.0 33 802692.0 34 1093311.0 35 1478046.0 36 2151269.0 37 3060116.0 38 2866757.0 39 751169.0 40 629.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.453370056559635 19.150157845826744 12.32978854599301 24.066683551620606 2 15.650918220089954 20.55853604716766 40.25693264756064 23.533613085181745 3 18.299607755862798 26.94756168804073 29.82001897855794 24.932811577538537 4 12.073937353616767 16.92529726688241 39.36347823657206 31.637287142928766 5 12.959852073081882 38.160526794907504 35.043867119242584 13.835754012768032 6 29.900792691305277 37.894417817194324 18.930645292748384 13.27414419875201 7 26.99027508682329 32.453749132725704 23.409822922362046 17.14615285808897 8 26.112253315028113 34.91064455652314 20.70762073217707 18.26948139627168 9 27.025775031048482 15.14471504339244 20.575599201983245 37.253910723575835 10-11 24.858955485007446 25.768372082612395 29.13990531345634 20.23276711892382 12-13 25.612514533478137 23.765680760358435 28.613070274390584 22.008734431772847 14-15 22.932639498009372 25.211112599547604 26.24288654254425 25.61336135989877 16-17 22.3156887774814 27.706827967125864 27.04884449073831 22.92863876465443 18-19 21.983586415832125 27.630105152063848 28.47109108722926 21.915217344874765 20-21 23.1031444474035 26.784422715568752 27.944167043142805 22.168265793884938 22-23 22.972021013929066 26.67197997100227 27.72403339394465 22.63196562112402 24-25 22.65813540530647 26.707405968056356 28.094820861531655 22.539637765105514 26-27 22.612091487773263 26.752872445889675 27.880650581863975 22.75438548447309 28-29 22.29823781263023 27.38285328830671 27.826501022436545 22.492407876626515 30-31 23.061908236546145 26.850517201135037 27.727894763650678 22.35967979866814 32-33 22.962002240766203 27.036421322158393 27.473511684211587 22.528064752863813 34-35 23.049750212348638 26.98247262496175 27.835125158421093 22.132652004268518 36-37 22.86670632043158 26.778831615613143 27.850055108388588 22.50440695556669 38-39 22.794698205526323 26.90437032195878 27.973963205487994 22.3269682670269 40-41 22.779331202366087 26.447931722169933 28.19845771268688 22.574279362777098 42-43 22.58723730739276 26.640312075780294 27.714249547176976 23.058201069649968 44-45 22.36449695932151 26.96242414379594 27.906417613552755 22.7666612833298 46-47 22.469839676706957 26.64113042749168 27.700065271003275 23.188964624798082 48-49 22.609510767393544 26.576971930215286 27.802484933897237 23.01103236849393 50-51 22.360461159977422 26.789065359064367 27.706679978358302 23.143793502599912 52-53 22.15936548351331 27.016500063950144 27.530862010496605 23.293272442039946 54-55 22.04803487779663 26.755999490510135 27.812770511913655 23.383195119779586 56-57 22.484714760970526 26.5912363413518 27.583437423810313 23.34061147386736 58-59 22.336166740003062 26.631827550393727 27.832544070474174 23.199461639129034 60-61 22.34079990697268 26.868753803306134 27.586163942041285 23.204282347679897 62-63 22.337120992937052 26.558035738572606 27.518168006207834 23.586675262282508 64-65 21.93777697876618 26.497120760817854 27.632096745082492 23.933005515333473 66-67 22.15539967405847 26.29300239633798 27.413849918514615 24.137748011088934 68-69 21.902827348488287 26.4167184807746 27.581556714568944 24.09889745616817 70-71 22.015349497338633 26.02587272585569 27.68674246392842 24.27203531287725 72-73 22.064927259186522 26.116859247537654 27.639295519040246 24.17891797423558 74-75 22.020406097941777 26.355215117342606 27.40114787855626 24.22323090615936 76-77 21.948194564029738 26.07143434466818 27.473721791835366 24.506649299466716 78-79 21.810368184546807 26.08097969403292 27.629959604917335 24.478692516502935 80-81 21.934586308598377 26.13289520617343 27.55752659888492 24.374991886343278 82-83 22.223827067405406 25.729586814869464 27.896288537083336 24.150297580641798 84-85 22.27640180832134 25.908219424696675 27.358897390667416 24.456481376314578 86-87 21.794723284142616 26.152693761306452 27.632578372939463 24.42000458161147 88-89 22.12414747298969 25.56011038872348 27.5146616301639 24.801080508122926 90-91 22.312474496487013 25.38642786878628 27.592833714529913 24.708263920196792 92-93 22.544251116454614 25.461937307925357 27.449254957174702 24.544556618445323 94-95 22.088026835313233 25.265645107915745 27.689418131146027 24.956909925624995 96-97 22.115464744263427 25.096375068435293 27.68730931466783 25.10085087263345 98-99 22.09437942670371 25.304589116516755 27.4126279050487 25.188403551730833 100 22.44352499138335 24.842710157449037 27.157190924926162 25.556573926241455 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 584.0 1 492.0 2 847.5 3 1276.0 4 1052.0 5 939.5 6 1259.5 7 2067.5 8 3259.5 9 4081.5 10 4071.5 11 4071.5 12 4709.5 13 5586.5 14 6469.0 15 7441.5 16 8430.5 17 9514.5 18 10713.5 19 11947.5 20 13281.0 21 15127.5 22 17952.0 23 21697.0 24 27020.5 25 34921.5 26 46119.0 27 60144.5 28 78910.0 29 106826.0 30 141310.5 31 175791.0 32 219602.5 33 262887.5 34 303128.0 35 345861.0 36 392626.0 37 449960.5 38 483005.5 39 485870.5 40 487573.5 41 493359.0 42 504370.5 43 526500.5 44 559575.0 45 603350.5 46 642892.0 47 680995.5 48 723990.0 49 728642.0 50 701450.5 51 688590.5 52 671879.5 53 642610.0 54 606411.5 55 547291.0 56 487787.0 57 424879.5 58 359786.5 59 308891.0 60 251191.0 61 194932.0 62 152183.0 63 117633.0 64 85954.0 65 58983.5 66 41300.0 67 30517.0 68 22477.5 69 16100.0 70 12289.0 71 9517.5 72 7066.5 73 5094.0 74 3709.0 75 2711.0 76 1942.5 77 1275.5 78 831.0 79 601.0 80 468.0 81 367.0 82 294.0 83 234.5 84 176.0 85 138.5 86 116.0 87 91.0 88 66.0 89 59.5 90 55.0 91 42.0 92 32.5 93 25.0 94 26.0 95 25.0 96 13.5 97 9.0 98 5.0 99 1.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21875845568335023 2 0.14809072910379306 3 0.02346114134126282 4 0.02260205318361384 5 0.027138533095227884 6 0.055377193471473886 7 0.0620088596092966 8 0.055420456903873476 9 0.04234871982885481 10-11 0.04459223782329063 12-13 0.042904963959706666 14-15 0.02094568177174386 16-17 0.01913479810130392 18-19 0.01879178088727861 20-21 0.022385736021615896 22-23 0.025951878949410586 24-25 0.02271021176461281 26-27 0.019944442336210517 28-29 0.01945309335395804 30-31 0.017762729245202672 32-33 0.02613729365969454 34-35 0.026616281661261412 36-37 0.025318378689273748 38-39 0.023720721935660355 40-41 0.024947549268705838 42-43 0.031078595688761878 44-45 0.040679987436299234 46-47 0.04557493578779558 48-49 0.041526714613262614 50-51 0.02899577044323881 52-53 0.04444081580989207 54-55 0.04268246630736592 56-57 0.04522264783825607 58-59 0.04878879076605076 60-61 0.025046437114190617 62-63 0.026316527879635693 64-65 0.02722815020519846 66-67 0.0325464621451765 68-69 0.03671520288139404 70-71 0.040683077681470635 72-73 0.044601508558804834 74-75 0.025914796007353795 76-77 0.01910698589476133 78-79 0.01839931975051091 80-81 0.02173060404527926 82-83 0.020015517975152698 84-85 0.012104490336370715 86-87 0.013794854445126084 88-89 0.019236776191960098 90-91 0.020105135085123276 92-93 0.020346174208492412 94-95 0.02439439538302538 96-97 0.02522876157930317 98-99 0.03064905160993739 100 0.030105168459771126 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.6179946E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.532997786529506 #Duplication Level Percentage of deduplicated Percentage of total 1 71.5963685135863 40.475573427021246 2 15.71565328607695 17.769059848713066 3 5.777081530674473 9.797872121586613 4 2.634907200501039 5.958368117345436 5 1.3772537394579545 3.8930141302133014 6 0.8391866856981747 2.8465043427035965 7 0.4929620026297887 1.9508033862459095 8 0.33206473939066405 1.5018092141565524 9 0.23584988341499327 1.1999970843347751 >10 0.931669711956023 8.883997961216535 >50 0.04213736671056219 1.641027589640643 >100 0.02269542156389316 2.3810497183766413 >500 0.0014640305522003376 0.5591856517651744 >1k 6.577596259332365E-4 0.5915222530398285 >5k 0.0 0.0 >10k+ 4.8128160955076555E-5 0.5502151536405078 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 27158 0.1678497567297196 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20393 0.12603873956068828 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.2360980685596849E-5 2 0.0 0.0 0.0 0.0 1.2360980685596849E-5 3 0.0 0.0 0.0 6.1804903427984245E-6 1.2360980685596849E-5 4 0.0 0.0 0.0 1.8541471028395274E-5 1.2360980685596849E-5 5 0.0 6.1804903427984245E-6 0.0 2.4721961371193698E-5 1.2360980685596849E-5 6 0.0 6.1804903427984245E-6 0.0 2.4721961371193698E-5 3.090245171399212E-5 7 0.0 6.1804903427984245E-6 0.0 2.4721961371193698E-5 3.090245171399212E-5 8 0.0 6.1804903427984245E-6 0.0 2.4721961371193698E-5 4.9443922742387396E-5 9 0.0 6.1804903427984245E-6 0.0 4.3263432399588976E-5 4.9443922742387396E-5 10-11 0.0 6.1804903427984245E-6 0.0 7.41658841135811E-5 6.180490342798424E-5 12-13 0.0 6.1804903427984245E-6 0.0 1.0506833582757322E-4 1.4524152305576298E-4 14-15 0.0 6.1804903427984245E-6 0.0 1.2979029719876691E-4 2.03956181312348E-4 16-17 0.0 6.1804903427984245E-6 0.0 1.7614397476975512E-4 2.564903492261346E-4 18-19 0.0 9.270735514197637E-6 0.0 2.4103912336913855E-4 2.9048304611152596E-4 20-21 0.0 1.2360980685596849E-5 0.0 5.315221694806645E-4 3.213854978255181E-4 22-23 0.0 1.2360980685596849E-5 0.0 0.0013751591012726495 3.49197704368111E-4 24-25 0.0 1.545122585699606E-5 0.0 0.0031458695844843983 3.615586850537078E-4 26-27 0.0 1.8541471028395274E-5 0.0 0.005584073024718377 3.9555138193909917E-4 28-29 0.0 1.8541471028395274E-5 0.0 0.01526581114671211 4.264538336530913E-4 30-31 0.0 1.8541471028395274E-5 0.0 0.05222514339664669 4.449953046814866E-4 32-33 0.0 1.8541471028395274E-5 0.0 0.13040216574270397 4.882587370810755E-4 34-35 0.0 1.8541471028395274E-5 0.0 0.23965778377752311 5.624246211946566E-4 36-37 0.0 1.8541471028395274E-5 0.0 0.4170162248996381 5.778758470516527E-4 38-39 0.0 1.8541471028395274E-5 0.0 0.7143627055368418 6.118685439370441E-4 40-41 0.0 1.8541471028395274E-5 0.0 1.062920729154473 6.180490342798425E-4 42-43 0.0 1.8541471028395274E-5 0.0 1.3998439797017863 6.304100149654393E-4 44-45 0.0 1.8541471028395274E-5 0.0 1.774699989728025 6.520417311652338E-4 46-47 0.0 2.1631716199794488E-5 0.0 2.212167457171983 6.891246732220243E-4 48-49 0.0 3.090245171399212E-5 0.0 2.690095504645071 7.663808025070046E-4 50-51 0.0 3.090245171399212E-5 0.0 3.206209711701139 7.972832542209968E-4 52-53 0.0 3.090245171399212E-5 0.0 3.7088010058871643 8.930808545343724E-4 54-55 0.0 3.090245171399212E-5 0.0 4.224479488374065 9.517955127909574E-4 56-57 0.0 3.090245171399212E-5 0.0 4.795405374035241 9.734272289907519E-4 58-59 0.0 3.090245171399212E-5 0.0 5.43670232273952 9.981491903619456E-4 60-61 0.0 3.090245171399212E-5 0.0 6.107591459205118 0.0010105101710475425 62-63 0.0 3.3992696885391335E-5 0.0 6.783357002551183 0.0010445028679329337 64-65 0.0 3.708294205679055E-5 0.0 7.417784336239441 0.0010723150744755268 66-67 0.0 3.708294205679055E-5 0.0 8.088290282303785 0.0010846760551611236 68-69 0.0 3.708294205679055E-5 0.0 8.818657367583302 0.0010970370358467205 70-71 0.0 4.3263432399588976E-5 0.0 9.592467119482352 0.0011186687520465149 72-73 0.0 4.9443922742387396E-5 0.0 10.349648262114101 0.0011959248813314953 74-75 0.0 5.5624413085185823E-5 0.0 11.069357091797464 0.0012484590492452818 76-77 0.0 5.5624413085185823E-5 0.0 11.805413318437527 0.0012639102751022778 78-79 0.0 5.5624413085185823E-5 0.0 12.59756367542883 0.0012762712557878747 80-81 0.0 5.5624413085185823E-5 0.0 13.420681379282724 0.0012948127268162701 82-83 0.0 5.5624413085185823E-5 0.0 14.249803429504647 0.0013597078754156535 84-85 0.0 6.180490342798424E-5 0.0 15.033909878314798 0.0013720688561012504 86-87 0.0 6.180490342798424E-5 0.0 15.807815427814159 0.0014215127788436378 88 0.0 6.180490342798424E-5 0.0 16.39529575685852 0.0014771371919288234 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 13485 0.0 35.320343 2 GTACATG 56630 0.0 28.301506 1 GTATCAA 56010 0.0 27.53816 1 TACATGG 57060 0.0 27.301819 2 ACCTGGG 17230 0.0 27.200937 3 GTACTAG 7230 0.0 26.65059 1 ACATGGG 56460 0.0 26.468153 3 GGTATCA 39165 0.0 25.164331 1 CATGGGG 42280 0.0 24.259865 4 CAACGCA 62160 0.0 24.10133 5 ATCAACG 62990 0.0 23.74666 3 TATCAAC 65770 0.0 23.063639 2 AACGCAG 66020 0.0 23.041798 6 TCAACGC 65785 0.0 22.901808 4 GTACCTG 21695 0.0 22.80087 1 ATGGGGG 31140 0.0 22.462301 5 GCATCGT 7510 0.0 21.594728 8 TGGGGGG 32550 0.0 21.5766 6 ACGCAGA 71220 0.0 20.917658 7 CGCAGAG 72070 0.0 20.53283 8 >>END_MODULE