FastQCFastQC Report
Mon 6 Jun 2016
ERR1211420_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211420_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16179946
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT245030.15144055486958982No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT193860.11981498578549027No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG129000.034.679772
GTATCAA535450.027.502671
ACCTGGG165750.026.4529763
GTACATG559750.026.2751181
TACATGG566650.025.4264812
GTACTAG70100.025.2198121
ACATGGG551450.025.1824383
GGTATCA375200.024.8503151
CAACGCA602200.023.7601245
ATCAACG607550.023.5146243
CATGGGG409200.023.4862424
TGGGGGG287350.023.2527586
TATCAAC643250.022.6908442
AACGCAG645600.022.5768996
TCAACGC636850.022.5070484
ATGGGGG296850.022.1295345
GTACCTG216300.021.2813031
CCTGGGG160200.020.4468354
ACGCAGA702050.020.3934387
CGCAGAG711000.020.0572998
Produced by FastQC (version 0.11.5)