FastQCFastQC Report
Mon 6 Jun 2016
ERR1211419_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211419_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12526075
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT325370.2597541528371816No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT239890.19151250491474783No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151320.1208040028500548No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA175100.049.853241
GTATCAA328000.043.532761
TACCTGG110450.042.7406772
ATCAACG401900.034.610453
CAACGCA402450.034.4695665
TATCAAC412450.034.279532
ACCTGGG134400.034.2773363
TCAACGC422600.033.003744
AACGCAG430500.032.6935736
GTACATG464400.030.1684441
TACATGG466350.029.4395162
ACGCAGA478050.028.9403747
ACATGGG467200.028.2234613
CGCAGAG489050.028.1731828
ATGGGGG294600.027.5254785
CATGGGG371300.027.4225984
TGGGGGG312300.027.4107996
CCTGGGG131450.026.391914
GTACCTG183400.026.2002241
GCAGAGT535000.024.6721089
Produced by FastQC (version 0.11.5)