##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211419_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 12526075 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.205716475432247 31.0 31.0 34.0 28.0 34.0 2 31.266633402721922 31.0 31.0 34.0 28.0 34.0 3 31.327350826176595 31.0 31.0 34.0 28.0 34.0 4 34.96151986955211 37.0 35.0 37.0 32.0 37.0 5 35.01058096810054 37.0 35.0 37.0 32.0 37.0 6 35.07167784002571 37.0 35.0 37.0 32.0 37.0 7 34.993304367090246 37.0 35.0 37.0 32.0 37.0 8 34.99362889013518 37.0 35.0 37.0 32.0 37.0 9 36.53565454461992 39.0 37.0 39.0 32.0 39.0 10-11 36.452533535045895 39.0 37.0 39.0 32.0 39.0 12-13 36.35348171713805 39.0 35.0 39.0 32.0 39.0 14-15 37.51187139626739 40.0 37.0 41.0 32.0 41.0 16-17 37.49392874463868 40.0 37.0 41.0 32.0 41.0 18-19 37.480391742824466 40.0 37.0 41.0 31.5 41.0 20-21 37.467372341296056 40.0 37.0 41.0 31.0 41.0 22-23 37.35243629788262 40.0 37.0 41.0 31.0 41.0 24-25 37.259097722151594 40.0 36.0 41.0 31.0 41.0 26-27 37.1189067604976 40.0 36.0 41.0 30.5 41.0 28-29 36.985248731146825 39.0 36.0 41.0 30.0 41.0 30-31 36.83052069383267 39.0 36.0 41.0 30.0 41.0 32-33 36.64231121879759 39.0 35.0 41.0 30.0 41.0 34-35 36.530515784074424 39.0 35.0 41.0 30.0 41.0 36-37 36.413094125653885 39.0 35.0 40.5 29.5 41.0 38-39 36.266841807988534 39.0 35.0 40.0 29.0 41.0 40-41 36.10452468151436 38.5 35.0 40.0 28.5 41.0 42-43 35.916221042904496 38.0 35.0 40.0 27.5 41.0 44-45 35.79503671341581 38.0 35.0 40.0 27.0 41.0 46-47 35.5848393850428 38.0 34.0 40.0 26.5 41.0 48-49 35.351759310079174 38.0 34.0 40.0 26.0 41.0 50-51 34.706925553295825 37.5 33.5 39.5 25.0 40.5 52-53 35.03311476260521 38.0 33.5 39.5 26.0 40.5 54-55 35.45532674840283 38.0 34.0 40.0 26.0 41.0 56-57 35.38152501881076 38.0 34.0 40.0 26.0 41.0 58-59 35.17823651862216 38.0 34.0 40.0 26.0 41.0 60-61 34.94989240444433 37.5 34.0 40.0 26.0 41.0 62-63 34.66020145975495 37.0 33.0 40.0 26.0 41.0 64-65 34.447014168444625 37.0 33.0 40.0 25.5 41.0 66-67 34.25235889933599 36.0 33.0 39.5 25.5 41.0 68-69 33.91947844795756 36.0 33.0 39.0 25.0 41.0 70-71 33.55886069658692 35.0 32.0 39.0 24.0 40.5 72-73 33.18676884019934 35.0 32.0 38.0 24.0 40.0 74-75 32.82796251020372 35.0 32.0 37.0 23.0 39.5 76-77 32.46651664627587 35.0 31.0 37.0 22.5 39.0 78-79 32.10005895701566 35.0 31.0 36.0 21.5 39.0 80-81 31.74408599661107 35.0 31.0 36.0 20.5 38.0 82-83 31.409021181814737 34.5 30.5 35.0 20.0 37.0 84-85 31.10405346447311 34.0 30.0 35.0 20.0 37.0 86-87 30.795022503058618 34.0 30.0 35.0 19.0 36.0 88-89 30.52516638292522 34.0 29.5 35.0 18.0 36.0 90-91 30.260409865021565 34.0 29.0 35.0 18.0 36.0 92-93 29.999135044297596 34.0 29.0 35.0 17.0 35.0 94-95 29.74953263492355 34.0 29.0 35.0 15.0 35.0 96-97 29.485568025099642 34.0 28.5 35.0 14.0 35.0 98-99 29.206688767231554 34.0 27.0 35.0 10.5 35.0 100 27.96053065305772 32.0 25.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3497874755564254 1101 2 -0.32221846074766347 1101 3 -0.3501821245993355 1101 4 -0.31881029629496993 1101 5 -0.24169395050692088 1101 6 -0.3172171474371126 1101 7 -0.2717184521264855 1101 8 -0.3084338712639578 1101 9 -0.4972197873145703 1101 10-11 -0.43752967770105755 1101 12-13 -0.4632957673344009 1101 14-15 -0.7023626300780705 1101 16-17 -0.673444594633466 1101 18-19 -0.6566590864959352 1101 20-21 -0.7250371321847027 1101 22-23 -0.6791294582223131 1101 24-25 -0.7746211656917623 1101 26-27 -0.815135513390473 1101 28-29 -0.8184823275825011 1101 30-31 -0.8766522816466704 1101 32-33 -0.8800364533517708 1101 34-35 -0.8239135513251696 1101 36-37 -0.9163259778419572 1101 38-39 -0.9198493844395443 1101 40-41 -1.0700867477937166 1101 42-43 -0.9884579706844008 1101 44-45 -1.013010647477813 1101 46-47 -1.0792014543709954 1101 48-49 -1.1910601438694783 1101 50-51 -1.0856109350713936 1101 52-53 -0.854881472901063 1101 54-55 -0.8082503044963474 1101 56-57 -0.8676637970249175 1101 58-59 -0.817300317599674 1101 60-61 -0.9188111861696271 1101 62-63 -1.1309425681129923 1101 64-65 -0.9887337131675054 1101 66-67 -0.9423099375842909 1101 68-69 -0.9797759403298798 1101 70-71 -0.9834064469953319 1101 72-73 -1.0415455063329588 1101 74-75 -1.1612666244725567 1101 76-77 -0.9897528988446496 1101 78-79 -1.0484811369854619 1101 80-81 -1.1903093298745624 1101 82-83 -1.2843703258601167 1101 84-85 -1.2545619086525832 1101 86-87 -1.2793805076562919 1101 88-89 -1.4267665633502986 1101 90-91 -1.4468689268583006 1101 92-93 -1.612473609847246 1101 94-95 -1.7017736463786193 1101 96-97 -1.810905351094295 1101 98-99 -1.8685158525117984 1101 100 -1.9414437646652267 1102 1 -0.3861216279713382 1102 2 -0.3892573169440112 1102 3 -0.44102177352902316 1102 4 -0.4071372861099718 1102 5 -0.3448288213347226 1102 6 -0.36127118189627083 1102 7 -0.4350811004478814 1102 8 -0.39655988352389215 1102 9 -0.521986185019621 1102 10-11 -0.4683094524212308 1102 12-13 -0.5362750294405174 1102 14-15 -0.7651995566836973 1102 16-17 -0.8086099108015077 1102 18-19 -0.7736260791163758 1102 20-21 -0.8369156827035269 1102 22-23 -0.7935485114949259 1102 24-25 -0.8415805428153718 1102 26-27 -0.8724975110515771 1102 28-29 -0.9185145581298286 1102 30-31 -0.8429267666515372 1102 32-33 -0.9358762726939318 1102 34-35 -0.9315061448049775 1102 36-37 -0.9686461845735153 1102 38-39 -1.0848490082888844 1102 40-41 -1.2586550797227645 1102 42-43 -1.1042378486526943 1102 44-45 -1.1097645946415184 1102 46-47 -1.3208128132961434 1102 48-49 -1.2872569339777442 1102 50-51 -1.1364504370885484 1102 52-53 -0.9532216812051431 1102 54-55 -1.0077375767315075 1102 56-57 -0.9744790731202642 1102 58-59 -0.9755201783120455 1102 60-61 -1.0438848129991243 1102 62-63 -0.9966604024482422 1102 64-65 -1.100620769148641 1102 66-67 -1.1147468529038775 1102 68-69 -1.094941294855893 1102 70-71 -1.1616762408359662 1102 72-73 -1.1714166118887235 1102 74-75 -1.2044074474562407 1102 76-77 -1.2267457045577288 1102 78-79 -1.3373258714235519 1102 80-81 -1.3232146378197527 1102 82-83 -1.3909006841292957 1102 84-85 -1.4493008928432474 1102 86-87 -1.5065494058520308 1102 88-89 -1.5666111876570525 1102 90-91 -1.6316929336818333 1102 92-93 -1.7091711533636023 1102 94-95 -1.6869130459026849 1102 96-97 -1.7957762751698674 1102 98-99 -1.8201132981076924 1102 100 -1.8352374288811575 1103 1 -0.3672959843870629 1103 2 -0.40345565927685456 1103 3 -0.47228374232486203 1103 4 -0.2979379846843031 1103 5 -0.24868467717531217 1103 6 -0.2492780629067397 1103 7 -0.26196355205198074 1103 8 -0.30933683879208473 1103 9 -0.4750751116762473 1103 10-11 -0.47556272620897744 1103 12-13 -0.5614999425341693 1103 14-15 -0.7064230166238445 1103 16-17 -0.7143291680625907 1103 18-19 -0.7327379898868784 1103 20-21 -0.7037301047050377 1103 22-23 -0.7084148535784678 1103 24-25 -0.6845425745447713 1103 26-27 -0.7595217116408293 1103 28-29 -0.7565314651942714 1103 30-31 -0.808126385544945 1103 32-33 -0.8471470004575608 1103 34-35 -0.8005998311470179 1103 36-37 -0.8704130433200774 1103 38-39 -0.8745920319518277 1103 40-41 -0.7939637671604203 1103 42-43 -0.8389889084449678 1103 44-45 -0.8835945021554181 1103 46-47 -1.0596983811876086 1103 48-49 -0.9587010379360805 1103 50-51 -0.8706320837599648 1103 52-53 -0.6656200816132696 1103 54-55 -0.6811134006364412 1103 56-57 -0.7345458208045699 1103 58-59 -0.7021890989553086 1103 60-61 -0.7647519031357177 1103 62-63 -0.6710419911412302 1103 64-65 -0.7158186952137058 1103 66-67 -0.7757945730462481 1103 68-69 -0.7210915743640314 1103 70-71 -0.8120843607879209 1103 72-73 -0.7632961112970591 1103 74-75 -0.8122397691840106 1103 76-77 -0.8940373581012508 1103 78-79 -0.9107589260937807 1103 80-81 -0.9114532509119009 1103 82-83 -0.9158493831820103 1103 84-85 -1.063423861558963 1103 86-87 -1.0994637591505914 1103 88-89 -1.1147842278287428 1103 90-91 -1.1687877669094107 1103 92-93 -1.2050456883125555 1103 94-95 -1.2339743814096984 1103 96-97 -1.397637634904683 1103 98-99 -1.4612995256619001 1103 100 -1.4867845604762273 1104 1 -0.4915299668402824 1104 2 -0.39450403239551335 1104 3 -0.422996213382671 1104 4 -0.26477639742836345 1104 5 -0.33301026900940656 1104 6 -0.29448031208561787 1104 7 -0.27331963898145517 1104 8 -0.4585398359064783 1104 9 -0.4815438410541901 1104 10-11 -0.41472398566325097 1104 12-13 -0.49272654219192447 1104 14-15 -0.6215585722669417 1104 16-17 -0.655552136162683 1104 18-19 -0.6948166640088047 1104 20-21 -0.6706649387280379 1104 22-23 -0.7093038500635203 1104 24-25 -0.663828842337189 1104 26-27 -0.6910493673160332 1104 28-29 -0.7867824901169627 1104 30-31 -0.8032349426313559 1104 32-33 -0.820852334440211 1104 34-35 -0.8537102339042306 1104 36-37 -0.9068167843893775 1104 38-39 -0.9287428178022736 1104 40-41 -0.8736694953879933 1104 42-43 -0.9358880812227497 1104 44-45 -0.9520964167199537 1104 46-47 -0.9601989577586494 1104 48-49 -1.0322524712415913 1104 50-51 -0.9345864927653906 1104 52-53 -0.8259146870984253 1104 54-55 -0.7695081066207479 1104 56-57 -0.7509822125963268 1104 58-59 -0.8182187577860489 1104 60-61 -0.8299980497545008 1104 62-63 -0.8351492495538864 1104 64-65 -0.8516361830849135 1104 66-67 -0.8354296694041139 1104 68-69 -0.8574104581233968 1104 70-71 -0.8346527987396328 1104 72-73 -0.7971411773824642 1104 74-75 -0.7509340739984864 1104 76-77 -0.9313033836728479 1104 78-79 -0.8438384806262 1104 80-81 -0.8630348864910964 1104 82-83 -0.9204296689081914 1104 84-85 -0.9327762497392946 1104 86-87 -0.942924420724097 1104 88-89 -1.0018660922368454 1104 90-91 -1.046691382817734 1104 92-93 -1.0927181269747912 1104 94-95 -1.1162529786355364 1104 96-97 -1.2142785491997365 1104 98-99 -1.239565526653987 1104 100 -1.1871064174214396 1105 1 -0.5241841901921802 1105 2 -0.48076565819202344 1105 3 -0.523266122954908 1105 4 -0.3927684835599834 1105 5 -0.45472528839884063 1105 6 -0.40943631076254405 1105 7 -0.43807437582920983 1105 8 -0.4555821120185257 1105 9 -0.5761343682028581 1105 10-11 -0.5863434200733835 1105 12-13 -0.63781371983724 1105 14-15 -0.8004785349078318 1105 16-17 -0.8061323611615165 1105 18-19 -0.7186484436545371 1105 20-21 -0.8045597330879488 1105 22-23 -0.7436756867609446 1105 24-25 -0.811507136527112 1105 26-27 -0.9016537360599415 1105 28-29 -0.8371975614622613 1105 30-31 -0.8300995787983183 1105 32-33 -0.9556993716128304 1105 34-35 -0.9826799651995515 1105 36-37 -0.9814711474158386 1105 38-39 -0.9500645228449116 1105 40-41 -0.9939463272927327 1105 42-43 -0.9460913844493106 1105 44-45 -0.9783894616176525 1105 46-47 -1.1105014452704367 1105 48-49 -1.1277120504221045 1105 50-51 -1.0684568057054733 1105 52-53 -0.8896884020349276 1105 54-55 -0.882964368914223 1105 56-57 -0.9036828815760671 1105 58-59 -0.8843662495149331 1105 60-61 -0.8662234138868357 1105 62-63 -0.9429770876142882 1105 64-65 -0.9073774597564253 1105 66-67 -0.8963006860890843 1105 68-69 -0.9036901648682161 1105 70-71 -0.8130311771180132 1105 72-73 -0.8725691993221147 1105 74-75 -0.9592180799569086 1105 76-77 -0.9558894059332914 1105 78-79 -1.040524356381841 1105 80-81 -0.9739209834237066 1105 82-83 -1.0530462875713233 1105 84-85 -0.9580640982829536 1105 86-87 -0.938871249229063 1105 88-89 -1.0027252442587908 1105 90-91 -1.0394792639777108 1105 92-93 -1.0513398460616266 1105 94-95 -1.0773498536858988 1105 96-97 -1.1637601800767143 1105 98-99 -1.1690674794117513 1105 100 -1.2760015993398781 1106 1 -0.5265456408284521 1106 2 -0.6080595777102715 1106 3 -0.6076030457321089 1106 4 -0.425195775958791 1106 5 -0.46833599431955975 1106 6 -0.5426253360479691 1106 7 -0.508403993110953 1106 8 -0.5215490234615956 1106 9 -0.6730228729068628 1106 10-11 -0.8211219248582822 1106 12-13 -0.688742648742533 1106 14-15 -0.8713081068114974 1106 16-17 -0.8336555936122778 1106 18-19 -0.8003437011653176 1106 20-21 -0.9528407834882131 1106 22-23 -0.829238856609841 1106 24-25 -0.9225644776685655 1106 26-27 -0.9250831935657402 1106 28-29 -0.9171572454787409 1106 30-31 -0.8805532424717128 1106 32-33 -1.0478643119451831 1106 34-35 -1.0185644795057556 1106 36-37 -1.0101435858739407 1106 38-39 -1.096122533495432 1106 40-41 -1.1200034785668151 1106 42-43 -1.0258407776362688 1106 44-45 -1.111710189436991 1106 46-47 -1.0561353753089762 1106 48-49 -1.0343480058747474 1106 50-51 -1.0103268747071255 1106 52-53 -0.9259010067266757 1106 54-55 -0.8699240166370785 1106 56-57 -0.9504529302559419 1106 58-59 -0.8762748047886433 1106 60-61 -0.836802248125899 1106 62-63 -0.987182014767761 1106 64-65 -0.9414369706023606 1106 66-67 -0.9257377883318512 1106 68-69 -0.93119711823595 1106 70-71 -0.8892043552631677 1106 72-73 -0.8190651029389784 1106 74-75 -0.9177059982691134 1106 76-77 -0.9946587881543607 1106 78-79 -0.9761970679925724 1106 80-81 -0.9525848634826914 1106 82-83 -0.9969182859261281 1106 84-85 -1.0136987431423243 1106 86-87 -1.005802770940278 1106 88-89 -1.0360138139818673 1106 90-91 -1.0611246091347155 1106 92-93 -1.1307998627991331 1106 94-95 -1.0562031148492963 1106 96-97 -1.0741136901454098 1106 98-99 -1.16203900465419 1106 100 -1.130267645222002 1107 1 -0.6212605200992485 1107 2 -0.585704010327845 1107 3 -0.5814460150669944 1107 4 -0.4638890701841163 1107 5 -0.46549853745282377 1107 6 -0.49892640067338334 1107 7 -0.5640985711941724 1107 8 -0.5762819606986369 1107 9 -0.653661830347076 1107 10-11 -0.6393547831132125 1107 12-13 -0.7547294389013501 1107 14-15 -0.883428235792934 1107 16-17 -0.9551885407171312 1107 18-19 -0.9117484943356899 1107 20-21 -0.9171341974201823 1107 22-23 -0.8812822438630334 1107 24-25 -0.9278422107674444 1107 26-27 -0.9295972076051484 1107 28-29 -0.9040241787847947 1107 30-31 -0.9513087715172119 1107 32-33 -1.0158186067274713 1107 34-35 -1.0053527429237974 1107 36-37 -0.9607086323354181 1107 38-39 -1.0106846181166205 1107 40-41 -1.0158630012186407 1107 42-43 -1.0259525508081637 1107 44-45 -1.014340512753222 1107 46-47 -0.9526780223518685 1107 48-49 -1.0584916868802807 1107 50-51 -1.098084514090651 1107 52-53 -0.9138474038852991 1107 54-55 -0.8314495155765158 1107 56-57 -0.8578523230509063 1107 58-59 -0.8877428887048993 1107 60-61 -0.8418516927217112 1107 62-63 -0.8278012589306059 1107 64-65 -0.8013839189534337 1107 66-67 -0.7830313791819918 1107 68-69 -0.7617059428911475 1107 70-71 -0.7393334911294858 1107 72-73 -0.7834780001840826 1107 74-75 -0.6823918705119141 1107 76-77 -0.7150232018273925 1107 78-79 -0.7586614092688855 1107 80-81 -0.7234678012347224 1107 82-83 -0.8312807743486132 1107 84-85 -0.7995322839038046 1107 86-87 -0.8552475518784526 1107 88-89 -0.8248661560421411 1107 90-91 -0.9134039156901252 1107 92-93 -0.9771972645924656 1107 94-95 -0.8894834065249277 1107 96-97 -0.8734733715475365 1107 98-99 -0.874749709149814 1107 100 -0.9218220336455936 1108 1 -0.4944832658688547 1108 2 -0.45454631713899474 1108 3 -0.5310278381727933 1108 4 -0.36204171515650785 1108 5 -0.39171909226853785 1108 6 -0.39994954207802635 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1.2260929386712789 2311 68-69 1.2231610315446133 2311 70-71 1.192324618215146 2311 72-73 1.2445270542219689 2311 74-75 1.2444947538238367 2311 76-77 1.2185356065242416 2311 78-79 1.2469694148534423 2311 80-81 1.2060908823259702 2311 82-83 1.2799323548331287 2311 84-85 1.2289956006104568 2311 86-87 1.3135733288998104 2311 88-89 1.3283254022888755 2311 90-91 1.375088092244308 2311 92-93 1.3698437586988454 2311 94-95 1.4658896467464366 2311 96-97 1.4917290107078038 2311 98-99 1.5461281433780272 2311 100 1.5702743720647199 2312 1 0.5573163988900909 2312 2 0.6314564118216204 2312 3 0.6894579605677258 2312 4 0.5303817276542659 2312 5 0.5565558928971797 2312 6 0.42278805933512587 2312 7 0.5770187766967041 2312 8 0.6396012407520999 2312 9 0.7698829755064622 2312 10-11 0.8268980069539325 2312 12-13 0.8703245960136456 2312 14-15 1.067675828326415 2312 16-17 1.0263769936730966 2312 18-19 1.0507494236986687 2312 20-21 1.0791301892757375 2312 22-23 1.0735173916115315 2312 24-25 1.1235004960216557 2312 26-27 1.1417480835322067 2312 28-29 1.2014392692441263 2312 30-31 1.105618086297163 2312 32-33 1.1663901144014162 2312 34-35 1.1908802532394418 2312 36-37 1.2162284002206647 2312 38-39 1.2612093267140452 2312 40-41 1.304787410385316 2312 42-43 1.299127834781494 2312 44-45 1.329465854444237 2312 46-47 1.2909775251268272 2312 48-49 1.3477847873131736 2312 50-51 1.2742979530099277 2312 52-53 1.140172225385193 2312 54-55 1.10297217835096 2312 56-57 1.0928477041917262 2312 58-59 1.1401588962676499 2312 60-61 1.1301933604025933 2312 62-63 1.1452975763287654 2312 64-65 1.1300053515765214 2312 66-67 1.0860862626471643 2312 68-69 1.0980583404133526 2312 70-71 1.0453814123515528 2312 72-73 1.1065577661929424 2312 74-75 1.0779720014072467 2312 76-77 1.0835018716265665 2312 78-79 1.0765594592295322 2312 80-81 0.966549006648048 2312 82-83 1.1550531332389937 2312 84-85 1.2228210575395586 2312 86-87 1.2318927388219585 2312 88-89 1.248854581373326 2312 90-91 1.3324020159050391 2312 92-93 1.425206464937446 2312 94-95 1.470766769811803 2312 96-97 1.5373236658204625 2312 98-99 1.3828326829096298 2312 100 1.5391611286813855 2313 1 0.6609442962993946 2313 2 0.6536669259444814 2313 3 0.6934858864104001 2313 4 0.6270389834398742 2313 5 0.6240258790248632 2313 6 0.6222950147380075 2313 7 0.6612722874537766 2313 8 0.6862054786134522 2313 9 0.8680508646177856 2313 10-11 0.7968712760862857 2313 12-13 0.9218421259050515 2313 14-15 1.1221167562487437 2313 16-17 1.1098486051436112 2313 18-19 1.0842945172019185 2313 20-21 1.0870809074669623 2313 22-23 1.1401508261620208 2313 24-25 1.1722677510172517 2313 26-27 1.2100838694893241 2313 28-29 1.1580408663377355 2313 30-31 1.2064722337849716 2313 32-33 1.2427270168839755 2313 34-35 1.2385673439917966 2313 36-37 1.2887511934163243 2313 38-39 1.335610895411115 2313 40-41 1.3440113193145535 2313 42-43 1.3485475656625923 2313 44-45 1.3241194230993187 2313 46-47 1.3045960463374016 2313 48-49 1.3580327064194933 2313 50-51 1.3266588896095826 2313 52-53 1.2002890867240765 2313 54-55 1.1412408807045722 2313 56-57 1.2069834632255336 2313 58-59 1.186546513829235 2313 60-61 1.1968313573673655 2313 62-63 1.1926225973821616 2313 64-65 1.1761782272087018 2313 66-67 1.1492166331520295 2313 68-69 1.1527960830799273 2313 70-71 1.1464728284015635 2313 72-73 1.1553356929103487 2313 74-75 1.1553267366223992 2313 76-77 1.1352978653203962 2313 78-79 1.1684255907202434 2313 80-81 1.1804806402280796 2313 82-83 1.2040601060444232 2313 84-85 1.2701028070168796 2313 86-87 1.3949105912590234 2313 88-89 1.3767130743420495 2313 90-91 1.4193918196687392 2313 92-93 1.4926774961388354 2313 94-95 1.5190849101367512 2313 96-97 1.550058427586933 2313 98-99 1.62751894349595 2313 100 1.6815751642330419 2314 1 0.5176801150075967 2314 2 0.510079431833617 2314 3 0.5979078459072156 2314 4 0.48684662604505746 2314 5 0.5328500795281101 2314 6 0.5439903013849943 2314 7 0.5490750279014023 2314 8 0.5637447096064108 2314 9 0.7545481001558585 2314 10-11 0.7391287844296741 2314 12-13 0.4989913005101698 2314 14-15 0.8689257675284239 2314 16-17 0.914256620850793 2314 18-19 0.9130029666454718 2314 20-21 0.9307827688220343 2314 22-23 0.8220838649856077 2314 24-25 0.8783695146249428 2314 26-27 0.9826518929993995 2314 28-29 1.0398241502692116 2314 30-31 0.9791728729457461 2314 32-33 1.0278531483203892 2314 34-35 1.1470105374858264 2314 36-37 1.1392526680777095 2314 38-39 1.1965639006146844 2314 40-41 1.2108050382007107 2314 42-43 1.270783719937235 2314 44-45 1.3276969030339956 2314 46-47 1.3717503847221906 2314 48-49 1.3659571275585591 2314 50-51 1.2628021782453231 2314 52-53 1.0832356471503388 2314 54-55 1.0998011351294394 2314 56-57 1.0728835119583735 2314 58-59 1.0774189918871357 2314 60-61 1.1145892400099555 2314 62-63 1.0956713488848209 2314 64-65 1.0611075786746014 2314 66-67 1.0890184147880646 2314 68-69 1.1314262835676772 2314 70-71 1.1274778217571324 2314 72-73 1.123191331729572 2314 74-75 1.1794947659776014 2314 76-77 1.1756290268028664 2314 78-79 1.1970616011203425 2314 80-81 1.261574199535314 2314 82-83 1.1066856494472859 2314 84-85 1.1698048465425934 2314 86-87 1.3037415787962345 2314 88-89 1.4568356916617873 2314 90-91 1.4811216101527407 2314 92-93 1.573858010467486 2314 94-95 1.5439073644972474 2314 96-97 1.6493943193943927 2314 98-99 1.7218916560630397 2314 100 1.7093925843925213 2315 1 0.4135368590034716 2315 2 0.42504954960656605 2315 3 0.4965012879865185 2315 4 0.4010566600468408 2315 5 0.36303669025120655 2315 6 -0.2816898372586465 2315 7 0.265485406709999 2315 8 0.32654736177636323 2315 9 0.5187583530090336 2315 10-11 0.613826106512505 2315 12-13 0.6285348099699561 2315 14-15 0.7178898730673211 2315 16-17 0.6656791935442214 2315 18-19 0.7641399382673981 2315 20-21 0.8108258516503284 2315 22-23 0.6967541460474465 2315 24-25 0.8141419982587266 2315 26-27 0.8948670965286212 2315 28-29 0.966375536319461 2315 30-31 1.0003116713331508 2315 32-33 1.0385727342844433 2315 34-35 1.0496490742814402 2315 36-37 1.063456364400544 2315 38-39 1.1255418208261005 2315 40-41 1.184827023496986 2315 42-43 1.1566238286523145 2315 44-45 1.1861438331153522 2315 46-47 1.2393351008101376 2315 48-49 1.225692912130512 2315 50-51 1.187473244597463 2315 52-53 1.0446832294088537 2315 54-55 0.9069821901167572 2315 56-57 0.9101106300150548 2315 58-59 0.9208935320031131 2315 60-61 0.803940555461331 2315 62-63 0.9763676065774476 2315 64-65 1.0516346989970344 2315 66-67 1.0572695462497776 2315 68-69 1.0980948748392692 2315 70-71 1.1331182852174422 2315 72-73 1.1610908964470141 2315 74-75 1.1285849556676908 2315 76-77 1.1473849153215099 2315 78-79 1.051923404188063 2315 80-81 1.0604341639938504 2315 82-83 1.2008339557692054 2315 84-85 1.1629263729203636 2315 86-87 1.2351802962858578 2315 88-89 1.3741540462906485 2315 90-91 1.4306099999574542 2315 92-93 1.5396997361081688 2315 94-95 1.533701583679239 2315 96-97 1.6019545846319865 2315 98-99 1.688068868019844 2315 100 1.7372768913056014 2316 1 0.14030856006962367 2316 2 0.05836283689148303 2316 3 0.21046080431309022 2316 4 0.1642813775760601 2316 5 0.14312556581126756 2316 6 0.2373202972939623 2316 7 0.2450148905151437 2316 8 0.24476888774976402 2316 9 0.4035737790805456 2316 10-11 0.26961741277284546 2316 12-13 0.39421745699934263 2316 14-15 0.5832136716242218 2316 16-17 0.6531473252465503 2316 18-19 0.6032833221604292 2316 20-21 0.580853565112669 2316 22-23 0.4398962973279552 2316 24-25 0.5118831934872432 2316 26-27 0.5809805529213676 2316 28-29 0.7076930950310611 2316 30-31 0.6976566832586499 2316 32-33 0.7965874710300795 2316 34-35 0.8068183901732553 2316 36-37 0.838951212699591 2316 38-39 0.8788719190289243 2316 40-41 0.9270715466682091 2316 42-43 0.9496973353232008 2316 44-45 0.8109938146152302 2316 46-47 0.7987353066339224 2316 48-49 0.8752119782679486 2316 50-51 0.6679110484699535 2316 52-53 0.39180591260655007 2316 54-55 0.38626034742947013 2316 56-57 0.5111944925168785 2316 58-59 0.5638465995328446 2316 60-61 0.6503236221439437 2316 62-63 0.6546229046908465 2316 64-65 0.6695202913368519 2316 66-67 0.6746024644765996 2316 68-69 0.6918468274364571 2316 70-71 0.5295454375340469 2316 72-73 0.6311357453457234 2316 74-75 0.7020260913123053 2316 76-77 0.7863849682574795 2316 78-79 0.8477956840135796 2316 80-81 0.9458559056178366 2316 82-83 0.925638093650754 2316 84-85 0.9891221673596924 2316 86-87 1.0281351648888126 2316 88-89 1.0665569128827528 2316 90-91 1.1497636259943569 2316 92-93 1.2308927364655382 2316 94-95 1.2498003069255326 2316 96-97 1.304541315263176 2316 98-99 1.4689758051029003 2316 100 1.3848142256186406 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 59.0 1 706.0 2 341.0 3 247.0 4 233.0 5 152.0 6 229.0 7 1151.0 8 3983.0 9 7810.0 10 11286.0 11 15439.0 12 19826.0 13 24249.0 14 28924.0 15 34425.0 16 42568.0 17 53331.0 18 66086.0 19 78902.0 20 95006.0 21 112711.0 22 125486.0 23 131112.0 24 135941.0 25 146097.0 26 164129.0 27 190115.0 28 224495.0 29 266156.0 30 320771.0 31 393245.0 32 493144.0 33 636500.0 34 872369.0 35 1151693.0 36 1654529.0 37 2355565.0 38 2127534.0 39 538972.0 40 558.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.36687228476106 19.015577370808632 12.659458032306842 23.958092312123465 2 15.93103962158889 20.329251692413372 40.62900997275477 23.110698713242968 3 18.351864540985787 27.064094068014583 30.068244235516918 24.515797155482716 4 12.642424952859727 16.84961953203566 38.204439067073736 32.30351644803087 5 13.08627885207699 37.81257731033424 34.168640301177504 14.932503536411259 6 29.842036308053405 36.79219752434779 19.450011446412816 13.91575472118599 7 26.884851149545753 32.99471271581143 23.02320372292292 17.097232411719894 8 26.161447742140382 35.208046874388444 20.672227384939955 17.958277998531223 9 27.967387756344053 15.581943960694582 20.445196116963274 36.005472165998086 10-11 25.075278212507353 25.819091086617167 28.87784527123598 20.2277854296395 12-13 25.432675909944706 23.83262313365679 28.391510445300327 22.343190511098175 14-15 23.15964336758392 25.24466635998947 26.243709517551945 25.351980754874663 16-17 22.532780328828345 27.61770426209651 27.00658730651446 22.842928102560684 18-19 22.176839249056187 27.312722074909214 28.692025371080867 21.818413304953737 20-21 23.066031432535105 26.73241126194433 28.143801486525806 22.057755818994764 22-23 22.886135859517378 26.52855382378265 28.09467161251053 22.490638704189443 24-25 22.446784015926756 26.249351360542843 28.40073228135795 22.903132342172448 26-27 22.3109711484433 26.53349576725073 28.180219244267068 22.975313840038897 28-29 22.304961703251994 27.474885943374545 27.968689159169923 22.25146319420354 30-31 23.329523025652584 26.615498194934606 28.093441647637086 21.96153713177572 32-33 23.153396494670744 26.660039343112402 27.78390011549638 22.402664046720474 34-35 23.054073740254253 26.843335668134877 28.10354955980613 21.99904103180474 36-37 22.744242953885976 26.870937176245757 27.996046794530578 22.38877307533769 38-39 22.886218601032763 26.927245646630727 28.015613418372816 22.1709223339637 40-41 22.918087042040234 26.286311102891634 28.387161068291565 22.408440786776563 42-43 22.63169850227875 26.64685591296216 27.78516702591177 22.936278558847327 44-45 22.033343851570976 26.908543005256163 28.337250318641903 22.720862824530958 46-47 21.975401517214202 26.437175126415212 28.006276453228867 23.58114690314172 48-49 22.298440787493842 26.49723630885103 28.104560234155016 23.099762669500105 50-51 22.22755706650768 26.80893278926706 27.942373140685874 23.02113700353939 52-53 21.8685116492004 26.998607725112926 27.75481857137517 23.3780620543115 54-55 21.694957218743085 26.545739964394876 28.323288609954577 23.43601420690746 56-57 22.254025350484127 26.523219267642652 27.780831675010564 23.441923706862656 58-59 21.831536217470035 26.69212962972207 28.253174147681698 23.223160005126193 60-61 22.357656789451998 26.74586533134025 27.852762367552998 23.043715511654753 62-63 22.498104261466903 26.472203873503293 27.705731550647045 23.323960314382763 64-65 21.97499438921141 26.178256620081715 27.883836532863647 23.962912457843224 66-67 22.105206667976624 25.99305487980203 27.687704297454342 24.214034154767006 68-69 21.56686147372752 26.16244438730732 28.09071444078998 24.17997969817517 70-71 21.790476526672183 25.915473002604177 28.069147764950213 24.224902705773427 72-73 21.82177883320391 26.279594256850725 27.56137792613024 24.337248983815122 74-75 21.90542381711297 26.142904210996864 27.56318475673084 24.388487215159326 76-77 21.622113984886113 25.90151978091481 27.717706402246684 24.758659831952393 78-79 21.489142256596168 25.88370152350396 27.939059681996564 24.68809653790331 80-81 21.675025554168727 25.960064988756642 27.71720803868152 24.64770141839311 82-83 22.129828786683593 25.646284935095025 27.93894128189963 24.28494499632175 84-85 22.242448676807555 25.90725921950335 27.37697358469566 24.473318518993437 86-87 21.663557733438967 26.083225194355737 27.7358083435983 24.517408728606995 88-89 21.78760486896709 25.381611731462623 27.76609816696695 25.06468523260334 90-91 22.34752702019452 25.115034851012442 27.782371772452574 24.755066356340464 92-93 22.431403756052987 25.230102082696533 27.621169104150155 24.71732505710032 94-95 22.05848104515509 25.13451223307615 27.658168982284163 25.1488377394846 96-97 22.010943060391654 24.951307613468174 27.733487289991498 25.30426203614867 98-99 22.00084081457196 25.011610212610936 27.462378259469116 25.525170713347993 100 22.15804110641951 24.93109334291618 27.399075654578304 25.511789896086007 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1138.0 1 870.0 2 1483.5 3 2307.0 4 1785.5 5 1444.5 6 1932.0 7 3292.0 8 5388.5 9 6810.0 10 6584.0 11 6267.0 12 6859.0 13 7892.5 14 8982.0 15 9935.5 16 10976.5 17 12000.5 18 13081.0 19 14329.5 20 15641.0 21 17324.0 22 19908.0 23 23883.5 24 29449.5 25 36208.0 26 45465.5 27 57002.5 28 72061.5 29 92810.5 30 117321.0 31 140677.5 32 168505.5 33 197362.5 34 223091.0 35 248745.0 36 277474.5 37 316520.0 38 341538.5 39 346615.5 40 351156.0 41 357506.0 42 369178.0 43 388465.0 44 413276.5 45 446533.5 46 479146.5 47 508480.5 48 537975.0 49 556149.0 50 566423.5 51 574204.0 52 563544.5 53 534571.0 54 496764.5 55 444783.0 56 392461.5 57 342755.0 58 290718.0 59 239733.5 60 187422.5 61 143414.0 62 111443.5 63 86275.5 64 63686.0 65 44641.5 66 32066.5 67 24022.0 68 17835.5 69 13077.0 70 9987.0 71 7467.5 72 5548.0 73 4110.5 74 3023.5 75 2190.0 76 1533.0 77 1055.0 78 755.0 79 528.0 80 367.0 81 270.5 82 228.5 83 194.0 84 152.5 85 114.5 86 94.5 87 74.5 88 53.0 89 44.0 90 37.5 91 38.5 92 31.5 93 18.5 94 16.0 95 13.5 96 10.0 97 8.0 98 4.5 99 2.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21694744762425583 2 0.14527296060417969 3 0.023678606426993293 4 0.022457154375971723 5 0.02788583015828981 6 0.05483760874815135 7 0.06178312041082302 8 0.05451827487860324 9 0.04196047045862331 10-11 0.04406807399764092 12-13 0.0423157293884956 14-15 0.020692834746718347 16-17 0.018900573403879507 18-19 0.018269889011521965 20-21 0.02166281137547077 22-23 0.024768333256826258 24-25 0.02192626181784797 26-27 0.01930373241418401 28-29 0.01885267332344729 30-31 0.017180162181688996 32-33 0.02531918418179677 34-35 0.025870035106767282 36-37 0.02453282452803452 38-39 0.022932163506924556 40-41 0.023766423241119026 42-43 0.029949525290244552 44-45 0.039645299904399425 46-47 0.04493027544542085 48-49 0.04102641889019505 50-51 0.028588364671295673 52-53 0.04437942452045034 54-55 0.043149989122690066 56-57 0.04505401731987075 58-59 0.048383072909909924 60-61 0.024704466482916635 62-63 0.025953860247523667 64-65 0.026548619579557047 66-67 0.03229662923142325 68-69 0.03624838586708127 70-71 0.04018816748263123 72-73 0.044646866636196894 74-75 0.0255227595236337 76-77 0.018856664996816642 78-79 0.01794656346860449 80-81 0.02137940256624681 82-83 0.019623066283732133 84-85 0.011963045087946544 86-87 0.013431980887867909 88-89 0.018768848182690905 90-91 0.0197907165652449 92-93 0.019982316886973772 94-95 0.023610747979714318 96-97 0.02450887448781841 98-99 0.029494474526138476 100 0.02953838293320134 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.2526075E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.427126936019214 #Duplication Level Percentage of deduplicated Percentage of total 1 73.98409490661597 42.48694009448728 2 14.933067699198594 17.151263486120918 3 5.101469232840364 8.788881635835603 4 2.2518055451228824 5.172588915000149 5 1.127112180841556 3.2363407140160736 6 0.6560607239982323 2.2605409484869896 7 0.42285345690501475 1.6998281401513171 8 0.27412163208655144 1.2593614209394517 9 0.19518606766704763 1.0088077575652181 >10 0.9807343510000516 9.776399545172742 >50 0.04795974098326973 1.865201932528311 >100 0.022429618207096328 2.4023886743228946 >500 0.001874706374658516 0.7389154203914031 >1k 0.0011383369486142121 1.2390721676238012 >5k 3.6720484045289074E-5 0.15792442967274 >10k+ 5.5080726067933615E-5 0.7555447176852186 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 32537 0.2597541528371816 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23989 0.19151250491474783 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15132 0.1208040028500548 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.596669347740613E-5 2 0.0 0.0 0.0 0.0 1.596669347740613E-5 3 0.0 0.0 0.0 0.0 1.596669347740613E-5 4 0.0 0.0 0.0 7.983346738703066E-6 1.596669347740613E-5 5 0.0 0.0 0.0 7.983346738703066E-6 1.596669347740613E-5 6 0.0 0.0 0.0 7.983346738703066E-6 3.193338695481226E-5 7 0.0 0.0 0.0 7.983346738703066E-6 3.193338695481226E-5 8 0.0 0.0 0.0 7.983346738703066E-6 3.193338695481226E-5 9 0.0 0.0 0.0 1.596669347740613E-5 3.193338695481226E-5 10-11 0.0 0.0 0.0 1.9958366846757664E-5 3.193338695481226E-5 12-13 0.0 0.0 0.0 2.3950040216109197E-5 8.382514075638218E-5 14-15 0.0 0.0 0.0 3.5925060324163795E-5 1.0378350760313985E-4 16-17 0.0 0.0 0.0 9.180848749508526E-5 1.1975020108054598E-4 18-19 0.0 0.0 0.0 1.6765028151276436E-4 1.3172522118860057E-4 20-21 0.0 0.0 0.0 3.9517566356580175E-4 1.3970856792730365E-4 22-23 0.0 0.0 0.0 0.0011855269906974053 1.476919146660067E-4 24-25 0.0 0.0 0.0 0.003057621800923274 1.596669347740613E-4 26-27 3.991673369351533E-6 0.0 0.0 0.006215035436080337 1.6365860814341284E-4 28-29 7.983346738703066E-6 7.983346738703066E-6 0.0 0.018102238730009202 1.7962530162081897E-4 30-31 7.983346738703066E-6 7.983346738703066E-6 0.0 0.061503703274968416 1.8760864835952202E-4 32-33 1.1975020108054598E-5 7.983346738703066E-6 0.0 0.16161087970493548 1.9958366846757662E-4 34-35 1.596669347740613E-5 7.983346738703066E-6 0.0 0.3033631843973471 2.075670152062797E-4 36-37 1.596669347740613E-5 7.983346738703066E-6 0.0 0.5223823105002964 2.075670152062797E-4 38-39 1.596669347740613E-5 7.983346738703066E-6 0.0 0.8801080945148421 2.1555036194498276E-4 40-41 1.596669347740613E-5 7.983346738703066E-6 0.0 1.3071692449550238 2.3550872879174044E-4 42-43 1.596669347740613E-5 7.983346738703066E-6 0.0 1.715317846971218 2.434920755304435E-4 44-45 1.596669347740613E-5 7.983346738703066E-6 0.0 2.152669531357588 2.5945876900784965E-4 46-47 2.3950040216109197E-5 7.983346738703066E-6 0.0 2.6468027694229836 2.7542546248525575E-4 48-49 2.3950040216109197E-5 7.983346738703066E-6 0.0 3.180118273281934 2.794171358546073E-4 50-51 2.3950040216109197E-5 7.983346738703066E-6 0.0 3.7519095167480634 3.07358849440068E-4 52-53 2.3950040216109197E-5 7.983346738703066E-6 0.0 4.342653225371874 3.392922363948803E-4 54-55 2.3950040216109197E-5 1.1975020108054598E-5 0.0 4.946585422808022 3.632422766109895E-4 56-57 2.3950040216109197E-5 1.596669347740613E-5 0.0 5.58954820244969 3.7521729671904404E-4 58-59 2.3950040216109197E-5 1.596669347740613E-5 0.0 6.319258027754104 3.8320064345774714E-4 60-61 2.3950040216109197E-5 3.193338695481226E-5 0.0 7.0589310697884216 3.8320064345774714E-4 62-63 2.3950040216109197E-5 3.193338695481226E-5 0.0 7.835415323634898 3.8320064345774714E-4 64-65 2.3950040216109197E-5 3.193338695481226E-5 0.0 8.560434932730324 3.871923168270987E-4 66-67 2.3950040216109197E-5 3.193338695481226E-5 0.0 9.335534075917636 3.951756635658017E-4 68-69 2.3950040216109197E-5 3.193338695481226E-5 0.0 10.165818103436232 3.9916733693515325E-4 70-71 2.794171358546073E-5 3.193338695481226E-5 0.0 11.003223276245752 4.1513403041255935E-4 72-73 3.193338695481226E-5 3.193338695481226E-5 0.0 11.794951730689782 4.710174575834809E-4 74-75 3.193338695481226E-5 3.193338695481226E-5 0.0 12.559073772111375 4.7900080432218393E-4 76-77 3.5925060324163795E-5 4.790008043221839E-5 0.0 13.393385398059648 5.029508445382931E-4 78-79 3.991673369351533E-5 5.1891753801569926E-5 0.0 14.258165466836179 5.029508445382931E-4 80-81 3.991673369351533E-5 5.987510054027299E-5 0.0 15.100779773392702 5.149258646463476E-4 82-83 3.991673369351533E-5 7.185012064832759E-5 0.0 15.96872523915113 5.428675782318084E-4 84-85 3.991673369351533E-5 7.185012064832759E-5 0.0 16.80889264993224 5.428675782318084E-4 86-87 3.991673369351533E-5 7.983346738703066E-5 0.0 17.645423646273873 5.4685925160116E-4 88 3.991673369351533E-5 7.983346738703066E-5 0.0 18.274814736459746 5.508509249705115E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 17510 0.0 49.85324 1 GTATCAA 32800 0.0 43.53276 1 TACCTGG 11045 0.0 42.740677 2 ATCAACG 40190 0.0 34.61045 3 CAACGCA 40245 0.0 34.469566 5 TATCAAC 41245 0.0 34.27953 2 ACCTGGG 13440 0.0 34.277336 3 TCAACGC 42260 0.0 33.00374 4 AACGCAG 43050 0.0 32.693573 6 GTACATG 46440 0.0 30.168444 1 TACATGG 46635 0.0 29.439516 2 ACGCAGA 47805 0.0 28.940374 7 ACATGGG 46720 0.0 28.223461 3 CGCAGAG 48905 0.0 28.173182 8 ATGGGGG 29460 0.0 27.525478 5 CATGGGG 37130 0.0 27.422598 4 TGGGGGG 31230 0.0 27.410799 6 CCTGGGG 13145 0.0 26.39191 4 GTACCTG 18340 0.0 26.200224 1 GCAGAGT 53500 0.0 24.672108 9 >>END_MODULE