FastQCFastQC Report
Mon 6 Jun 2016
ERR1211419_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211419_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12526075
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT291880.23301792460926504No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT227810.18186862205439455No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151950.12130695369459309No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA174450.049.5913351
TACCTGG111650.042.216712
GTATCAA318050.041.4072761
ACCTGGG133700.034.410973
ATCAACG398500.032.2887153
CAACGCA399450.032.1597255
TATCAAC412650.031.6709652
TCAACGC416800.030.9954414
AACGCAG429250.030.4077516
GTACATG443350.027.2655541
TGGGGGG267300.027.0170126
ACGCAGA481700.026.7455467
TACATGG444950.026.7420462
CCTGGGG128350.026.6920814
CGCAGAG493000.026.0752288
ACATGGG440750.025.7282713
CATGGGG346500.025.3487684
ATGGGGG272550.024.790715
GTACCTG192950.024.7819291
TATCACG9300.024.7602632
Produced by FastQC (version 0.11.5)