##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211419_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 12526075 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.01644609344906 33.0 31.0 34.0 30.0 34.0 2 32.15718179876777 33.0 31.0 34.0 30.0 34.0 3 32.23453428148881 34.0 31.0 34.0 30.0 34.0 4 35.895061461790704 37.0 35.0 37.0 35.0 37.0 5 35.74392185900212 37.0 35.0 37.0 35.0 37.0 6 35.776923258083634 37.0 35.0 37.0 33.0 37.0 7 35.703995944459855 37.0 35.0 37.0 33.0 37.0 8 35.68544695764635 37.0 35.0 37.0 33.0 37.0 9 37.25852751161078 39.0 37.0 39.0 34.0 39.0 10-11 37.229115345389516 39.0 37.0 39.0 33.0 39.0 12-13 37.14086088419557 39.0 37.0 39.0 33.0 39.0 14-15 38.33172458252086 40.0 38.0 41.0 33.0 41.0 16-17 38.3117314881158 40.0 38.0 41.0 33.0 41.0 18-19 38.307185012064835 40.0 38.0 41.0 33.5 41.0 20-21 38.26605241466301 40.0 38.0 41.0 33.5 41.0 22-23 38.1733398530665 40.0 37.5 41.0 33.0 41.0 24-25 38.05777823460262 40.0 37.0 41.0 33.0 41.0 26-27 37.93620320012454 40.0 37.0 41.0 33.0 41.0 28-29 37.85710759355983 40.0 37.0 41.0 33.0 41.0 30-31 37.9178684863375 40.0 37.0 41.0 33.0 41.0 32-33 37.77286125142952 40.0 37.0 41.0 33.0 41.0 34-35 37.673832784810884 40.0 37.0 41.0 33.0 41.0 36-37 37.54690144358867 40.0 36.5 41.0 32.0 41.0 38-39 37.44073175356207 40.0 36.0 41.0 32.0 41.0 40-41 37.379981039551495 39.5 36.0 41.0 32.0 41.0 42-43 37.255980504667264 39.0 36.0 41.0 31.0 41.0 44-45 37.121818087469535 39.0 36.0 41.0 31.0 41.0 46-47 36.9228702925697 39.0 36.0 41.0 31.0 41.0 48-49 36.72350273329834 39.0 35.0 41.0 30.0 41.0 50-51 36.5534356931441 39.0 35.0 40.5 30.0 41.0 52-53 36.35530303786302 39.0 35.0 40.0 30.0 41.0 54-55 36.158493502553675 38.5 35.0 40.0 29.0 41.0 56-57 35.933850428007176 38.0 35.0 40.0 28.5 41.0 58-59 35.66765207776578 38.0 34.0 40.0 28.0 41.0 60-61 35.40507385593652 37.5 34.0 40.0 28.0 41.0 62-63 35.09710915031245 37.0 34.0 40.0 27.5 41.0 64-65 34.74273501475921 36.5 34.0 39.5 26.5 41.0 66-67 34.39157541368705 36.0 33.0 39.0 26.0 40.5 68-69 34.027612360615755 35.5 33.0 39.0 26.0 40.0 70-71 33.65808176144563 35.0 32.5 38.0 26.0 40.0 72-73 33.303954271389884 35.0 32.0 37.5 25.5 39.5 74-75 32.942265394387306 35.0 32.0 37.0 25.0 39.0 76-77 31.94052670130109 34.0 30.5 36.0 24.5 38.5 78-79 32.4441747714268 34.5 31.5 36.0 25.0 38.0 80-81 32.35304251331722 35.0 32.0 35.5 25.0 37.0 82-83 32.08696986087023 34.0 31.5 35.0 25.0 37.0 84-85 31.81540618270288 34.0 31.0 35.0 24.5 36.5 86-87 31.567956163443057 34.0 31.0 35.0 24.0 36.0 88-89 31.35424308891652 34.0 31.0 35.0 23.5 36.0 90-91 31.14403442419114 34.0 31.0 35.0 23.0 35.0 92-93 30.963114064062367 34.0 30.5 35.0 22.5 35.0 94-95 30.799583428967175 34.0 30.0 35.0 20.5 35.0 96-97 30.631704224986677 34.0 30.0 35.0 20.0 35.0 98-99 30.44616302393208 34.0 29.5 35.0 20.0 35.0 100 28.593770834040193 32.0 25.0 34.0 17.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.29034769615299183 1101 2 -0.2495390804463007 1101 3 -0.3035592432898433 1101 4 -0.18555567926348004 1101 5 -0.14118377222170864 1101 6 -0.2543615225806093 1101 7 -0.25832576191017864 1101 8 -0.31277222725152853 1101 9 -0.48058113540076874 1101 10-11 -0.4218598199104733 1101 12-13 -0.41250099160124165 1101 14-15 -0.607233375373859 1101 16-17 -0.6250828826773542 1101 18-19 -0.6233629876564066 1101 20-21 -0.6745573662735396 1101 22-23 -0.730341123524056 1101 24-25 -0.7399355633392659 1101 26-27 -0.7504158383176005 1101 28-29 -0.7783840449567876 1101 30-31 -0.6754137679469281 1101 32-33 -0.6407357156709494 1101 34-35 -0.7287909459223201 1101 36-37 -0.7038700926546184 1101 38-39 -0.7644327549846395 1101 40-41 -0.7274700193493686 1101 42-43 -0.690771340166279 1101 44-45 -0.765496276318288 1101 46-47 -0.834676777315515 1101 48-49 -0.7768072168456683 1101 50-51 -0.8395010600147401 1101 52-53 -0.8030208522799214 1101 54-55 -0.8138878670990763 1101 56-57 -0.851036563098603 1101 58-59 -0.7995801635208082 1101 60-61 -0.7634676858596237 1101 62-63 -0.858423609389888 1101 64-65 -0.8648151986229422 1101 66-67 -0.8494441005331552 1101 68-69 -0.9056647262954485 1101 70-71 -0.9521302388453776 1101 72-73 -1.0118660328300777 1101 74-75 -1.0734977272176707 1101 76-77 -0.868387701976225 1101 78-79 -0.7890188408334637 1101 80-81 -0.6785499723143715 1101 82-83 -0.8085665475812256 1101 84-85 -0.7636002106889848 1101 86-87 -0.8783223172477754 1101 88-89 -0.9479561047752867 1101 90-91 -0.943717511956006 1101 92-93 -1.0887158863677193 1101 94-95 -1.119273842637181 1101 96-97 -1.1627275577068694 1101 98-99 -1.364296286307411 1101 100 -1.2193342152749267 1102 1 -0.31802879905037784 1102 2 -0.2759201438971388 1102 3 -0.32244065704140823 1102 4 -0.23340778925641814 1102 5 -0.18998980544176902 1102 6 -0.31779639758988054 1102 7 -0.3121803024943759 1102 8 -0.3838246306935318 1102 9 -0.4606888830824829 1102 10-11 -0.4497166265560182 1102 12-13 -0.46907402803643095 1102 14-15 -0.6073403152640751 1102 16-17 -0.7051166942400329 1102 18-19 -0.6922478096573954 1102 20-21 -0.7112913891264583 1102 22-23 -0.7340594015988202 1102 24-25 -0.7738096396513683 1102 26-27 -0.8611198959264712 1102 28-29 -0.8778988181867646 1102 30-31 -0.7962856395401019 1102 32-33 -0.7270166642207201 1102 34-35 -0.7953174124497551 1102 36-37 -0.7683545925032647 1102 38-39 -0.8614543096151621 1102 40-41 -0.873417635040866 1102 42-43 -0.9109353723278986 1102 44-45 -0.9419433541340467 1102 46-47 -0.9682241529073607 1102 48-49 -0.9468686331061207 1102 50-51 -1.0301513577953685 1102 52-53 -0.9717247186750839 1102 54-55 -1.0560579863820365 1102 56-57 -1.0026134758110672 1102 58-59 -1.0944265704875562 1102 60-61 -1.105352456736874 1102 62-63 -1.1275291102347182 1102 64-65 -1.0605468506199287 1102 66-67 -1.0265378436452082 1102 68-69 -1.0696795207056908 1102 70-71 -1.0899072550060893 1102 72-73 -1.18443631410193 1102 74-75 -1.1093949778368355 1102 76-77 -0.9518364680277074 1102 78-79 -0.8824569160797857 1102 80-81 -0.830095580831987 1102 82-83 -0.831158253218117 1102 84-85 -0.899500536768091 1102 86-87 -0.9451500151083998 1102 88-89 -0.918206298331409 1102 90-91 -0.9277193324281647 1102 92-93 -0.9831930311898454 1102 94-95 -1.076836293274038 1102 96-97 -1.2098710990120516 1102 98-99 -1.125989363925413 1102 100 -1.1206834438871063 1103 1 -0.3111470091401074 1103 2 -0.2705687177274747 1103 3 -0.31738637256930247 1103 4 -0.19476670073919422 1103 5 -0.18542230938658122 1103 6 -0.2985419092034718 1103 7 -0.2994832726369978 1103 8 -0.3182177325867812 1103 9 -0.4245267021662471 1103 10-11 -0.42459033944270885 1103 12-13 -0.5125474868944337 1103 14-15 -0.6545681298821648 1103 16-17 -0.7298642970906641 1103 18-19 -0.7423046075671635 1103 20-21 -0.6814976178448333 1103 22-23 -0.7687098232379341 1103 24-25 -0.6906210392315941 1103 26-27 -0.7888089813714529 1103 28-29 -0.8226031592464054 1103 30-31 -0.6544264389370795 1103 32-33 -0.677033587963713 1103 34-35 -0.6655592669941299 1103 36-37 -0.652360212269592 1103 38-39 -0.7466816792396003 1103 40-41 -0.7737666270792616 1103 42-43 -0.7564062180401194 1103 44-45 -0.744961939497415 1103 46-47 -0.806518617227006 1103 48-49 -0.8102796150201144 1103 50-51 -0.8684940037782454 1103 52-53 -0.8898995942450156 1103 54-55 -0.8473853104597922 1103 56-57 -0.9373530815801701 1103 58-59 -1.0115488957922878 1103 60-61 -0.8635364342293599 1103 62-63 -0.9453584906637786 1103 64-65 -0.9120022493832423 1103 66-67 -0.9570046621362067 1103 68-69 -0.9470354239333574 1103 70-71 -0.878233089243551 1103 72-73 -0.9408185645190272 1103 74-75 -0.9435031276073893 1103 76-77 -0.8829628652618879 1103 78-79 -0.8348880234147273 1103 80-81 -0.8073897393376868 1103 82-83 -0.7346672343800371 1103 84-85 -0.7462452793215171 1103 86-87 -0.8033436917429846 1103 88-89 -0.8273505779170947 1103 90-91 -0.9653320371858563 1103 92-93 -0.8710201522946797 1103 94-95 -0.9958898504277833 1103 96-97 -1.0892520547673463 1103 98-99 -1.1825709972842482 1103 100 -1.164805035789282 1104 1 -0.3597993914804647 1104 2 -0.3342518429785777 1104 3 -0.362896797219868 1104 4 -0.22636880203694432 1104 5 -0.26004661039964816 1104 6 -0.36418662180030736 1104 7 -0.3378378189884046 1104 8 -0.3573894336578505 1104 9 -0.4679623165794524 1104 10-11 -0.4333598646768664 1104 12-13 -0.5235370173310727 1104 14-15 -0.653779179873716 1104 16-17 -0.7070731171180071 1104 18-19 -0.7700208149729235 1104 20-21 -0.6829548088808153 1104 22-23 -0.753037678482336 1104 24-25 -0.8369246376761197 1104 26-27 -0.8838558539800303 1104 28-29 -0.882753849247969 1104 30-31 -0.7790189418412652 1104 32-33 -0.7491928668030354 1104 34-35 -0.8119734695084517 1104 36-37 -0.8172803454987871 1104 38-39 -0.952040091757695 1104 40-41 -0.9374387270904236 1104 42-43 -0.7981077197137125 1104 44-45 -0.8447618876971177 1104 46-47 -0.8333424087146213 1104 48-49 -0.8402144321704128 1104 50-51 -0.8713248005394476 1104 52-53 -0.8644434824629172 1104 54-55 -0.7909790643003376 1104 56-57 -0.8694519883455101 1104 58-59 -0.8904435449668711 1104 60-61 -0.883637459397093 1104 62-63 -0.9460920563315085 1104 64-65 -0.9311795637118578 1104 66-67 -0.9412354675844128 1104 68-69 -0.8299407463782273 1104 70-71 -0.9371034185420655 1104 72-73 -0.9425658424501293 1104 74-75 -0.8674882920564926 1104 76-77 -0.7699739167274586 1104 78-79 -0.7126749483630732 1104 80-81 -0.8311984775006636 1104 82-83 -0.7865506218350617 1104 84-85 -0.6973201953218755 1104 86-87 -0.6620536818038083 1104 88-89 -0.7781638100157515 1104 90-91 -0.8158135318986233 1104 92-93 -0.9568757646001025 1104 94-95 -1.0051185374361857 1104 96-97 -1.0413072780879382 1104 98-99 -1.0403772431518874 1104 100 -1.0995594017113461 1105 1 -0.40067937189532543 1105 2 -0.35068432452733944 1105 3 -0.3949054478331675 1105 4 -0.21382832903135096 1105 5 -0.2754998351552018 1105 6 -0.3230556876461179 1105 7 -0.3649887112555774 1105 8 -0.3476198890045836 1105 9 -0.5027864072174921 1105 10-11 -0.5121348574053499 1105 12-13 -0.569090493117109 1105 14-15 -0.783926398393028 1105 16-17 -0.7291050135685069 1105 18-19 -0.7026961429970839 1105 20-21 -0.7583570447481733 1105 22-23 -0.7548592803039398 1105 24-25 -0.7284653478214764 1105 26-27 -0.7818163751963638 1105 28-29 -0.8049267249092225 1105 30-31 -0.8046785395938869 1105 32-33 -0.764606951984554 1105 34-35 -0.7218965026872937 1105 36-37 -0.8592588432912365 1105 38-39 -0.8126180221030666 1105 40-41 -0.8325807385115027 1105 42-43 -0.7969876371982849 1105 44-45 -0.8663548069644591 1105 46-47 -0.919591294963503 1105 48-49 -0.8446314482153383 1105 50-51 -0.8947141495101931 1105 52-53 -0.8233447808991059 1105 54-55 -0.8905027126611955 1105 56-57 -0.9025448819035233 1105 58-59 -0.9119086082924 1105 60-61 -0.9606846317931002 1105 62-63 -0.9420151708592712 1105 64-65 -0.9683479613453798 1105 66-67 -0.9013305997725709 1105 68-69 -0.9175488502653977 1105 70-71 -0.9239057076699382 1105 72-73 -0.9702663003831447 1105 74-75 -0.9685772164012327 1105 76-77 -0.9040677089607883 1105 78-79 -0.913632179552863 1105 80-81 -0.735541358587728 1105 82-83 -0.7896560784288909 1105 84-85 -0.6552237415975135 1105 86-87 -0.774021565864718 1105 88-89 -0.8006982278990087 1105 90-91 -0.8153049476691088 1105 92-93 -0.9089878050842408 1105 94-95 -0.8788678553715492 1105 96-97 -0.9899754965235985 1105 98-99 -0.9575477742759091 1105 100 -0.9441679253896389 1106 1 -0.3987768521496733 1106 2 -0.36047096001243517 1106 3 -0.3961855385521993 1106 4 -0.2495005853140313 1106 5 -0.28725530029645796 1106 6 -0.3615484138688956 1106 7 -0.36286915917283835 1106 8 -0.4061860618525017 1106 9 -0.569301679517153 1106 10-11 -0.5537714691050795 1106 12-13 -0.6073280698559103 1106 14-15 -0.7367117417632087 1106 16-17 -0.733858505813096 1106 18-19 -0.7539585112873652 1106 20-21 -0.7162008727963141 1106 22-23 -0.7259819994806307 1106 24-25 -0.8201928306225668 1106 26-27 -0.7922413430349522 1106 28-29 -0.7676137072526146 1106 30-31 -0.7478770281876734 1106 32-33 -0.8064669286178301 1106 34-35 -0.7861169658107414 1106 36-37 -0.8429730298058971 1106 38-39 -0.8396211638224216 1106 40-41 -0.7995664044749731 1106 42-43 -0.7167207504180766 1106 44-45 -0.839613069963967 1106 46-47 -0.8901827170339018 1106 48-49 -0.9096787738195076 1106 50-51 -0.9935645434330596 1106 52-53 -1.0314628667371082 1106 54-55 -0.9516700243685747 1106 56-57 -1.0363354928003687 1106 58-59 -1.0204404259756856 1106 60-61 -1.1123958938638765 1106 62-63 -1.0876398462446275 1106 64-65 -1.1173052512646393 1106 66-67 -1.0367631214151274 1106 68-69 -1.040106197617888 1106 70-71 -1.0445729794366443 1106 72-73 -1.023885356047792 1106 74-75 -1.0686455023470742 1106 76-77 -1.042321021041353 1106 78-79 -0.9090366596184012 1106 80-81 -0.7879239775577673 1106 82-83 -0.8409294873192721 1106 84-85 -0.8269282042500699 1106 86-87 -0.8082125250565788 1106 88-89 -0.8540358864067699 1106 90-91 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0.9616647823694251 2115 54-55 0.9279571559690254 2115 56-57 0.92407486918637 2115 58-59 0.8441383242419249 2115 60-61 0.8895077725043308 2115 62-63 0.9234882469823091 2115 64-65 1.0276589275828343 2115 66-67 0.993428641428828 2115 68-69 1.054460789639137 2115 70-71 1.1005339955174875 2115 72-73 1.0409356670163348 2115 74-75 0.6985286996183433 2115 76-77 0.6954980225212637 2115 78-79 0.5259182709867574 2115 80-81 0.72283150198475 2115 82-83 0.772510658033088 2115 84-85 0.8544493258969403 2115 86-87 0.8923418418595297 2115 88-89 0.8985319955625819 2115 90-91 0.9513797479909663 2115 92-93 0.9844079704506434 2115 94-95 0.9497615707628349 2115 96-97 0.9796104947737163 2115 98-99 1.0580196241358841 2115 100 1.016179051096973 2116 1 0.19103944844841791 2116 2 0.32475249587330524 2116 3 0.3745671623498552 2116 4 0.22933728177776658 2116 5 0.3195607653011052 2116 6 0.28822631655831543 2116 7 0.36000947361829105 2116 8 0.4142543942032262 2116 9 0.5852648437463301 2116 10-11 0.5640651925862059 2116 12-13 0.46255909715951304 2116 14-15 0.6299249761982324 2116 16-17 0.7302753046039285 2116 18-19 0.6556169987068188 2116 20-21 0.7972243493831357 2116 22-23 0.8293226168841485 2116 24-25 0.8613010705067126 2116 26-27 0.8683004199008764 2116 28-29 0.8554087331261329 2116 30-31 0.683011617950136 2116 32-33 0.7531180945360418 2116 34-35 0.7334022471869446 2116 36-37 0.8030096784120104 2116 38-39 0.6982312576561043 2116 40-41 0.8199101279239898 2116 42-43 0.8955191406592178 2116 44-45 0.9267505830530851 2116 46-47 0.8550214196206269 2116 48-49 0.7814393798121273 2116 50-51 0.892007373940892 2116 52-53 0.859317398134543 2116 54-55 0.8643572697877815 2116 56-57 0.964589021969914 2116 58-59 0.9017329097155411 2116 60-61 0.9345342752586845 2116 62-63 0.9043447413182548 2116 64-65 0.7642774464188022 2116 66-67 0.77474247864604 2116 68-69 0.7506402293432899 2116 70-71 0.7034778147023459 2116 72-73 0.8097581483207392 2116 74-75 0.7700354087354313 2116 76-77 0.7497059210085979 2116 78-79 0.7352195669921358 2116 80-81 0.6754532773645323 2116 82-83 0.6407137758914594 2116 84-85 0.6487226895966707 2116 86-87 0.6516802385999547 2116 88-89 0.7025600909082428 2116 90-91 0.5135161789460945 2116 92-93 0.7053218376165553 2116 94-95 0.7860497764572365 2116 96-97 0.8738868175254062 2116 98-99 0.9339724962288187 2116 100 0.638961178352826 2201 1 0.7086367225927681 2201 2 0.6415614604535023 2201 3 0.6227986516202151 2201 4 0.4351034922532122 2201 5 0.5392104572781022 2201 6 0.5391123167515843 2201 7 0.5702417591352571 2201 8 0.6007900639520614 2201 9 0.6997682897628721 2201 10-11 0.6580782281537054 2201 12-13 0.6820861213268401 2201 14-15 0.853378129879637 2201 16-17 0.8469659785066952 2201 18-19 0.8061600971886804 2201 20-21 0.8045255831338949 2201 22-23 0.805332679440184 2201 24-25 0.7847042755419054 2201 26-27 0.8211016780994669 2201 28-29 0.8225525597530705 2201 30-31 0.9497275090589952 2201 32-33 0.8041961180113262 2201 34-35 0.9658520863872226 2201 36-37 0.9945269494657438 2201 38-39 1.0329596925563465 2201 40-41 1.0425536907300028 2201 42-43 0.04416550405835551 2201 44-45 0.7602780973582597 2201 46-47 0.9967139028825258 2201 48-49 1.0372057872132743 2201 50-51 1.060680267495286 2201 52-53 1.0593317087043346 2201 54-55 1.004229897572955 2201 56-57 1.0494424951700623 2201 58-59 1.05640028497821 2201 60-61 1.080704058565587 2201 62-63 1.0892524998163537 2201 64-65 1.0955196866946224 2201 66-67 1.077149720886844 2201 68-69 1.0738702459570035 2201 70-71 1.111052112259891 2201 72-73 1.0673395916383512 2201 74-75 1.03122245223539 2201 76-77 1.049653784986873 2201 78-79 0.8606817715124393 2201 80-81 0.7555779737709258 2201 82-83 0.7834890790912148 2201 84-85 0.7890188862449783 2201 86-87 0.719354653568562 2201 88-89 0.7427558521116637 2201 90-91 0.10585181228438856 2201 92-93 0.47132014525559285 2201 94-95 0.741243317686429 2201 96-97 0.740344222568531 2201 98-99 0.7607825079203963 2201 100 0.9411642512577743 2202 1 0.7199123854312823 2202 2 0.6689743424266652 2202 3 0.6336416525403905 2202 4 0.425429634846914 2202 5 0.42394845617144483 2202 6 0.480451433910261 2202 7 0.5459010515796976 2202 8 0.5496364998088623 2202 9 0.7354983091821623 2202 10-11 0.7165576058828336 2202 12-13 0.7612063534688644 2202 14-15 0.9165665265959007 2202 16-17 0.9280263739111589 2202 18-19 0.8937439178709923 2202 20-21 0.8783841511183752 2202 22-23 0.860640234004677 2202 24-25 0.8729958307472216 2202 26-27 0.8680852748874557 2202 28-29 0.8726007417910253 2202 30-31 1.0054897787701051 2202 32-33 0.6044769936010752 2202 34-35 0.9400138376500493 2202 36-37 1.0076763157873572 2202 38-39 1.0829381162551783 2202 40-41 1.0667480488763204 2202 42-43 1.1160639735747253 2202 44-45 1.0854389305451022 2202 46-47 1.1315683272955752 2202 48-49 1.1141956432788618 2202 50-51 1.1171546813830062 2202 52-53 1.1210788828070832 2202 54-55 1.0799365221181816 2202 56-57 1.0957436298592498 2202 58-59 1.1158274182970729 2202 60-61 1.128327246095651 2202 62-63 1.138362120069928 2202 64-65 1.1216815638285027 2202 66-67 1.1145244493366278 2202 68-69 1.1402056894377282 2202 70-71 1.136078148933116 2202 72-73 1.1214915515852866 2202 74-75 1.0659503344706707 2202 76-77 1.1041265211923559 2202 78-79 0.9901975116370565 2202 80-81 0.9367157876135721 2202 82-83 0.945538184507658 2202 84-85 0.9110079081717579 2202 86-87 0.9210132269666467 2202 88-89 0.9435031475270144 2202 90-91 0.9805363912252396 2202 92-93 0.9183131550915427 2202 94-95 0.9333442719488616 2202 96-97 0.7561281709105643 2202 98-99 0.789296771565013 2202 100 1.0423834748455505 2203 1 0.6453498562555389 2203 2 0.6211797551974954 2203 3 0.6035715843904725 2203 4 0.3610080541096323 2203 5 0.46342066038623386 2203 6 0.4767438330102749 2203 7 0.5203131399511918 2203 8 0.48570974934669664 2203 9 0.696025693307611 2203 10-11 0.6852093672204305 2203 12-13 0.6969550702745764 2203 14-15 0.8571634467629181 2203 16-17 0.7901622199438307 2203 18-19 0.8097070139662677 2203 20-21 0.8079858449935884 2203 22-23 0.8001231977857941 2203 24-25 0.7806753109963793 2203 26-27 0.7971279576820152 2203 28-29 0.844640691632371 2203 30-31 0.9018066797572359 2203 32-33 0.9616446196653712 2203 34-35 0.9899401744398943 2203 36-37 0.9992223210827476 2203 38-39 0.9896090987085273 2203 40-41 0.9581161388903112 2203 42-43 0.9995892972262084 2203 44-45 1.0266354560188375 2203 46-47 1.0225588262087015 2203 48-49 1.0232831091662433 2203 50-51 0.9815945184681922 2203 52-53 1.0099448997330285 2203 54-55 0.9903400769557109 2203 56-57 1.0411770693655669 2203 58-59 1.0598664315260677 2203 60-61 1.0787271369580367 2203 62-63 1.0604246441126435 2203 64-65 1.0595830248425315 2203 66-67 1.0618664934039828 2203 68-69 1.0692263967163598 2203 70-71 1.087068624163102 2203 72-73 1.059076034586063 2203 74-75 1.0431330886752974 2203 76-77 1.0465727694564464 2203 78-79 0.8920975430024782 2203 80-81 0.8343714976119045 2203 82-83 0.835199456933946 2203 84-85 0.8218009363386933 2203 86-87 0.7840594814229185 2203 88-89 0.873892636204733 2203 90-91 0.8961295779940137 2203 92-93 0.9277641592354051 2203 94-95 0.9653240957824742 2203 96-97 0.9037766870275732 2203 98-99 0.9140969132938785 2203 100 1.1178128273328056 2204 1 0.6796870862472524 2204 2 0.6458882262145167 2204 3 0.6159525399877737 2204 4 0.4252011927565249 2204 5 0.5151930667980835 2204 6 0.4986651067611234 2204 7 0.5571016589487741 2204 8 0.5579258338280013 2204 9 0.7338082585588381 2204 10-11 0.7250610944827045 2204 12-13 0.7327881831095198 2204 14-15 0.8913546067693758 2204 16-17 0.8363807670995911 2204 18-19 0.8460888790407495 2204 20-21 0.8552539943060324 2204 22-23 0.8858802324305586 2204 24-25 0.8526082929271226 2204 26-27 0.8690388556354662 2204 28-29 0.8791870836914981 2204 30-31 0.9933813748974316 2204 32-33 1.0258964166132003 2204 34-35 1.0104520727902795 2204 36-37 1.0540410998802159 2204 38-39 1.0696338762266322 2204 40-41 1.0921773388994183 2204 42-43 1.1642615500208962 2204 44-45 1.1584981000447883 2204 46-47 1.1022316664107095 2204 48-49 1.0980151559093514 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0.7461271326890184 2205 10-11 0.7166559236819623 2205 12-13 0.7415847566374936 2205 14-15 0.94232489970247 2205 16-17 0.9230213344169087 2205 18-19 0.8922002856328604 2205 20-21 0.8966379604080359 2205 22-23 0.9145466329612546 2205 24-25 0.9241180244697915 2205 26-27 0.9948619919734583 2205 28-29 1.0197455336843788 2205 30-31 1.0778281336153057 2205 32-33 1.115100964913779 2205 34-35 1.124093000196659 2205 36-37 1.1297083911860355 2205 38-39 1.1747687826573383 2205 40-41 1.1719239215103627 2205 42-43 1.2140798127694055 2205 44-45 1.1816237193920713 2205 46-47 1.212281411846753 2205 48-49 1.2084655074337505 2205 50-51 1.208002459990972 2205 52-53 1.2196694278781308 2205 54-55 1.1680576725673717 2205 56-57 1.2118299745975705 2205 58-59 1.2282221261759005 2205 60-61 1.2124623694670973 2205 62-63 1.2285751736438044 2205 64-65 1.2766868610571436 2205 66-67 1.2764336884277938 2205 68-69 1.2348190830360508 2205 70-71 1.2580508322949484 2205 72-73 1.271506889732514 2205 74-75 1.305818254647221 2205 76-77 1.2449882233595773 2205 78-79 1.1966480239490735 2205 80-81 1.110141295125537 2205 82-83 1.0389430054903706 2205 84-85 1.0644655645071843 2205 86-87 1.0989185221117772 2205 88-89 1.08483359919315 2205 90-91 1.1204082138152138 2205 92-93 1.2165587231766004 2205 94-95 1.2398129171538876 2205 96-97 1.2089219388773316 2205 98-99 1.223277026081064 2205 100 1.4612211897520098 2206 1 0.6919980573829996 2206 2 0.6469430438397765 2206 3 0.6122992161179539 2206 4 0.29341692850673695 2206 5 0.4558804238647909 2206 6 0.4748731854746566 2206 7 0.5231981383800672 2206 8 0.5451381026105224 2206 9 0.7413053513679273 2206 10-11 0.7204120350607965 2206 12-13 0.7545171458086628 2206 14-15 0.9275381136685183 2206 16-17 0.915546953179323 2206 18-19 0.8821927328396484 2206 20-21 0.8629888294833563 2206 22-23 0.8998332154643478 2206 24-25 0.9402401624715679 2206 26-27 0.9800732864772286 2206 28-29 0.9683510104143451 2206 30-31 1.0306280086369881 2206 32-33 1.0918278605257612 2206 34-35 1.115766285615173 2206 36-37 1.1069918484555572 2206 38-39 1.1338066701281875 2206 40-41 1.1597705674293906 2206 42-43 1.2014759715570165 2206 44-45 1.202914639686007 2206 46-47 1.2289188745735586 2206 48-49 1.2446993324499545 2206 50-51 1.2410883111401674 2206 52-53 1.2295948800350942 2206 54-55 1.2136135478717378 2206 56-57 1.200838840380527 2206 58-59 1.2495390852358028 2206 60-61 1.22758871613226 2206 62-63 1.2372369240634882 2206 64-65 1.2702552276984775 2206 66-67 1.335388309528362 2206 68-69 1.3250133694560802 2206 70-71 1.3395214074715156 2206 72-73 1.3283693260400469 2206 74-75 1.318554928950057 2206 76-77 1.3318407865542383 2206 78-79 1.1771490355929402 2206 80-81 1.1364298214234836 2206 82-83 1.089879213518973 2206 84-85 1.105676094715978 2206 86-87 1.1571885083430224 2206 88-89 1.1888755311067207 2206 90-91 1.178788300111524 2206 92-93 1.2634151165709717 2206 94-95 1.2738287647385 2206 96-97 1.243102070135592 2206 98-99 1.290996323918499 2206 100 1.5329554743243108 2207 1 0.7039688103555655 2207 2 0.6857531024719563 2207 3 0.6393788419376634 2207 4 0.41876184672780425 2207 5 0.5339879302192614 2207 6 0.5549864960134911 2207 7 0.5757929786426672 2207 8 0.567349806290494 2207 9 0.7802095730238818 2207 10-11 0.7312094007731744 2207 12-13 0.772778999874042 2207 14-15 0.929853675733618 2207 16-17 0.9230874487662604 2207 18-19 0.9464151973920778 2207 20-21 0.9184822131332169 2207 22-23 0.9173932192597434 2207 24-25 0.9709501904775024 2207 26-27 0.984990925685814 2207 28-29 0.9924944683333763 2207 30-31 1.0536748568487937 2207 32-33 1.1285175990550158 2207 34-35 1.1084960097099028 2207 36-37 1.121488145679713 2207 38-39 1.149241259540993 2207 40-41 1.1101578574366044 2207 42-43 1.2361318788854092 2207 44-45 1.2217172552921838 2207 46-47 1.1897283679659836 2207 48-49 1.2009555063571753 2207 50-51 1.2106018597560322 2207 52-53 1.2082804744901807 2207 54-55 1.156129734927191 2207 56-57 1.2475334147207562 2207 58-59 1.1130618845112323 2207 60-61 1.162007939303308 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2311 46-47 1.1299919337718123 2311 48-49 1.1304041151099966 2311 50-51 1.1583334408907646 2311 52-53 1.181263939597656 2311 54-55 1.1221911517511316 2311 56-57 1.1888809454762068 2311 58-59 1.1842118795753578 2311 60-61 1.2557115123659486 2311 62-63 1.297107200903561 2311 64-65 1.3079777572524165 2311 66-67 1.2981429047019617 2311 68-69 1.2873445276059527 2311 70-71 1.3007169290745324 2311 72-73 1.309308176042535 2311 74-75 1.209528515419258 2311 76-77 1.1122066114913451 2311 78-79 1.0767855114205034 2311 80-81 1.045091536677809 2311 82-83 1.0784222656733284 2311 84-85 1.1650139201713294 2311 86-87 1.1494477411197188 2311 88-89 1.1400544405754154 2311 90-91 1.2475692022599922 2311 92-93 1.291085017750465 2311 94-95 1.3145001404249292 2311 96-97 1.3622310881382234 2311 98-99 1.3238472730174742 2311 100 1.5091610054037936 2312 1 0.18659681352933077 2312 2 0.18433320937449338 2312 3 0.347070734779237 2312 4 0.25890570671045765 2312 5 0.31524119824437236 2312 6 0.4199956877766482 2312 7 0.46906693849974346 2312 8 0.2911969112908892 2312 9 0.4153661143124694 2312 10-11 0.2952495173298999 2312 12-13 0.46039824158702203 2312 14-15 0.8204750380661565 2312 16-17 0.87538060339336 2312 18-19 0.9161881314968383 2312 20-21 0.8843928407592117 2312 22-23 0.47436228742139974 2312 24-25 0.44072390997929034 2312 26-27 0.7938591195482587 2312 28-29 0.9553232218421357 2312 30-31 0.8838381009059688 2312 32-33 0.7700995209862711 2312 34-35 0.9418389074336488 2312 36-37 1.001677410178253 2312 38-39 0.996262515192825 2312 40-41 1.0189327389927811 2312 42-43 0.9007212592799902 2312 44-45 0.880360592367019 2312 46-47 0.9945160458768569 2312 48-49 1.056638862932708 2312 50-51 1.0611935350331052 2312 52-53 1.1204505202759663 2312 54-55 1.1181189394785136 2312 56-57 1.1046346485413991 2312 58-59 1.131445060964289 2312 60-61 1.1609143382089258 2312 62-63 1.1497099816119203 2312 64-65 1.1679291930496092 2312 66-67 1.1772418782779752 2312 68-69 1.1813263595354115 2312 70-71 1.0127167516624525 2312 72-73 1.118292579458391 2312 74-75 1.1301997136273911 2312 76-77 1.071201865388609 2312 78-79 0.9771221141175843 2312 80-81 0.8323295225064058 2312 82-83 0.881371320108844 2312 84-85 0.7252161953682581 2312 86-87 0.9077385047351179 2312 88-89 0.6019433782589019 2312 90-91 0.9203892313116881 2312 92-93 1.0177475203183057 2312 94-95 1.1154226479299858 2312 96-97 1.204860426041332 2312 98-99 1.242414304155787 2312 100 1.3367347412403134 2313 1 -0.3713890964830995 2313 2 -0.003447217969501537 2313 3 0.2927640458839633 2313 4 0.10211385095604442 2313 5 0.25983742135720433 2313 6 0.3771025112516355 2313 7 0.17357006442108514 2313 8 0.09472263087707944 2313 9 0.31218576626517347 2313 10-11 0.3927349871223029 2313 12-13 0.586794782328603 2313 14-15 0.6440813977494102 2313 16-17 0.7759079742880886 2313 18-19 0.6159776159007464 2313 20-21 0.8099467390314672 2313 22-23 0.8872246218416748 2313 24-25 0.7197829486330249 2313 26-27 0.9479971372303524 2313 28-29 0.8338738712702778 2313 30-31 0.8600592994572622 2313 32-33 1.0210949283707293 2313 34-35 1.004143683521768 2313 36-37 0.8522471134638749 2313 38-39 0.662106813206222 2313 40-41 0.7905177788041016 2313 42-43 0.8348298961189897 2313 44-45 1.0317991984762571 2313 46-47 1.081839458345435 2313 48-49 1.1547339928155722 2313 50-51 1.0626294064560753 2313 52-53 1.0212880202460397 2313 54-55 1.117648393891109 2313 56-57 1.191363685697958 2313 58-59 1.0812944920850924 2313 60-61 1.0813467215717907 2313 62-63 1.179827385284213 2313 64-65 1.2197366319118785 2313 66-67 1.2368120095373811 2313 68-69 1.1963736069954223 2313 70-71 1.1135673219596356 2313 72-73 1.2000110314454915 2313 74-75 0.8836309392143349 2313 76-77 1.020830541953302 2313 78-79 0.7915807917190705 2313 80-81 0.9365063368234203 2313 82-83 0.9550243167817349 2313 84-85 1.0188392246677047 2313 86-87 1.0706538340604048 2313 88-89 1.1344733230734825 2313 90-91 1.1967243106700742 2313 92-93 0.924436705637774 2313 94-95 0.9632654316453966 2313 96-97 1.2554501744851834 2313 98-99 1.3304337265053405 2313 100 1.4047105798728303 2314 1 -0.28170991280043367 2314 2 -0.12846954211753925 2314 3 0.1603884677976808 2314 4 -0.09891269013201054 2314 5 0.20514963196195168 2314 6 0.25260908279066996 2314 7 0.14788685394098877 2314 8 0.2004147428471157 2314 9 0.27784758218320604 2314 10-11 0.47834483777585746 2314 12-13 0.5592398821781117 2314 14-15 0.5851764658732677 2314 16-17 0.7482635046556183 2314 18-19 0.831360811310276 2314 20-21 0.5187887654021281 2314 22-23 0.7474740818719425 2314 24-25 0.8938316106982427 2314 26-27 0.862662993326218 2314 28-29 0.819636645859056 2314 30-31 0.7884455980642215 2314 32-33 0.9026364231168102 2314 34-35 0.8995672331739897 2314 36-37 0.8426728444740874 2314 38-39 0.8757777570247214 2314 40-41 0.9223331933114167 2314 42-43 0.7113296985819701 2314 44-45 0.7545849000354963 2314 46-47 0.8778489288546751 2314 48-49 0.8080400089974091 2314 50-51 0.9664956830487554 2314 52-53 1.0739960115868001 2314 54-55 1.0514159741576918 2314 56-57 0.9771041009271144 2314 58-59 0.9559752726133652 2314 60-61 1.0660524442298183 2314 62-63 0.7726590626164622 2314 64-65 0.9281926600304402 2314 66-67 1.0409113164324495 2314 68-69 1.0781531320896605 2314 70-71 1.1016101966479326 2314 72-73 1.0759230204284975 2314 74-75 1.0861369304663668 2314 76-77 0.906635341574713 2314 78-79 0.936196349819248 2314 80-81 0.9031053004054783 2314 82-83 0.9146230490236498 2314 84-85 0.8381948332049696 2314 86-87 0.9494521434815475 2314 88-89 0.9889523781634288 2314 90-91 1.113266363519859 2314 92-93 1.1685374438839737 2314 94-95 1.2449853347149293 2314 96-97 1.3304625513590587 2314 98-99 1.3304900386405905 2314 100 1.3985141499055125 2315 1 -0.1298480409876177 2315 2 0.09748696721814554 2315 3 0.11824417727013525 2315 4 -0.04993988846853625 2315 5 0.2619519710456686 2315 6 0.3619297577592704 2315 7 0.4440743210167284 2315 8 0.40598640329068303 2315 9 0.5913031710556638 2315 10-11 0.5728659188296632 2315 12-13 0.604213831137848 2315 14-15 0.7456354706298143 2315 16-17 0.7414808686343335 2315 18-19 0.814746791277912 2315 20-21 0.7898910799744883 2315 22-23 0.8854211115950505 2315 24-25 0.8173929524272268 2315 26-27 0.9461931641796397 2315 28-29 0.9597269902899583 2315 30-31 0.8843258878955638 2315 32-33 0.9284641899005237 2315 34-35 0.9398721034672022 2315 36-37 1.001249392512996 2315 38-39 0.9651542403866102 2315 40-41 0.9602383580498781 2315 42-43 1.0184187201205503 2315 44-45 0.9169773088272493 2315 46-47 0.9484234317916602 2315 48-49 1.0376365963574798 2315 50-51 0.9770176140183864 2315 52-53 1.0289687980594238 2315 54-55 0.9300439767344741 2315 56-57 1.002450478318579 2315 58-59 1.0306496410468569 2315 60-61 1.1090482726647437 2315 62-63 1.138739761107729 2315 64-65 1.1809601131717855 2315 66-67 1.1839810715815062 2315 68-69 1.0417974931646015 2315 70-71 0.6870934681621819 2315 72-73 0.9238146640127738 2315 74-75 1.0717484810825724 2315 76-77 1.001474375085749 2315 78-79 0.8657961233981695 2315 80-81 0.9036776161158642 2315 82-83 0.9083413187896632 2315 84-85 0.8490431359737762 2315 86-87 0.8533104420947488 2315 88-89 0.9609866327838894 2315 90-91 1.0500505327882799 2315 92-93 1.101547932787689 2315 94-95 1.171770737275338 2315 96-97 1.2515016243148445 2315 98-99 1.15916166345 2315 100 1.0472924699198067 2316 1 0.5037157279095972 2316 2 0.4963739880348257 2316 3 0.49141634194567274 2316 4 0.31449500325422974 2316 5 0.4186739402284019 2316 6 0.45511159158097314 2316 7 0.5154514166146313 2316 8 0.5386148877711889 2316 9 0.5451960557417692 2316 10-11 0.5248909087279614 2316 12-13 0.656736869001854 2316 14-15 0.828897045125359 2316 16-17 0.85552920763147 2316 18-19 0.9066126503692828 2316 20-21 0.934258890834009 2316 22-23 0.9099190974451759 2316 24-25 1.0009895700527025 2316 26-27 1.060557847419652 2316 28-29 1.004390342753787 2316 30-31 0.8483905886300889 2316 32-33 0.8292418433415136 2316 34-35 0.7905724336898672 2316 36-37 0.8027210921694703 2316 38-39 0.7831646101158327 2316 40-41 0.8667653912083892 2316 42-43 0.9603133015456251 2316 44-45 0.9112941360289497 2316 46-47 0.944638945437525 2316 48-49 0.7393929281035696 2316 50-51 0.8050469166242067 2316 52-53 0.9672096024231536 2316 54-55 1.067393547886624 2316 56-57 1.0905305795112241 2316 58-59 1.0126840402484376 2316 60-61 1.0493998495074095 2316 62-63 1.077820393218822 2316 64-65 1.0670405043357434 2316 66-67 1.0598856060578115 2316 68-69 1.03258963676452 2316 70-71 0.9287861452029276 2316 72-73 0.9515185073864316 2316 74-75 1.0250337934570055 2316 76-77 0.8598788018081649 2316 78-79 0.8582227720986282 2316 80-81 0.8123090661425323 2316 82-83 0.7690815834967744 2316 84-85 0.8397855457048031 2316 86-87 0.7971544323426194 2316 88-89 0.7449569742638715 2316 90-91 0.8346299103347512 2316 92-93 0.8814788939147498 2316 94-95 0.8098252426132078 2316 96-97 0.9515669031332479 2316 98-99 1.058235362643714 2316 100 0.6359210398345958 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 2.0 10 2.0 11 14.0 12 53.0 13 316.0 14 1204.0 15 3424.0 16 8117.0 17 16210.0 18 26668.0 19 38124.0 20 52472.0 21 70704.0 22 86041.0 23 93501.0 24 100562.0 25 114554.0 26 137367.0 27 169672.0 28 210489.0 29 258674.0 30 316160.0 31 391996.0 32 495641.0 33 644554.0 34 898631.0 35 1210188.0 36 1805841.0 37 2720150.0 38 2329392.0 39 325323.0 40 28.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.0713385991551 19.95083759793542 13.546562106371304 24.431261696538183 2 15.570919062834927 20.74906944114577 40.2100258860018 23.469985610017506 3 17.83355121217141 26.977636649948206 30.796773929582887 24.392038208297492 4 12.532142763894836 17.316503094049178 38.124996856087364 32.026357285968615 5 12.87915948138213 37.92513731877116 34.309126908092225 14.886576291754485 6 29.895966580422545 37.223659358149895 19.060166591742934 13.820207469684629 7 26.7205644812662 33.07924949040196 22.89723599197161 17.30295003636023 8 26.124376328882416 35.58945597540738 20.409773788716667 17.876393906993542 9 27.614145058493595 15.546795514160513 20.278255724018297 36.56080370332759 10-11 24.982756843005582 25.953147710916273 28.865158572179183 20.198936873898965 12-13 25.266090648031142 23.809565056925607 28.444337343126318 22.480006951916934 14-15 23.007465788753713 25.312422386876136 26.14040747493767 25.539704349432473 16-17 22.45550467986174 27.71986764079013 26.96773994737134 22.85688773197679 18-19 22.142271222230427 27.361683528160256 28.645932584628465 21.850112664980852 20-21 22.94969244947658 26.756775787333808 28.07729350430047 22.21623825888914 22-23 22.717652793684596 26.58045928836122 28.075057675693117 22.62683024226106 24-25 22.30417533359439 26.32388543409283 28.40012006963081 22.971819162681975 26-27 22.282360344088715 26.563383157944486 28.13749593647554 23.016760561491264 28-29 22.23253279684815 27.48095457639026 28.001313271022816 22.285199355738776 30-31 23.21510729262875 26.608531214770714 28.063012569797944 22.11334892280259 32-33 23.10732388897408 26.633969791533467 27.820914904354417 22.43779141513804 34-35 23.004857256965174 26.886083336151433 28.109319004644213 21.999740402239183 36-37 22.647621644899093 26.90586741248533 28.00624008889771 22.44027085371787 38-39 22.789183708489116 26.838314174899086 28.118078791218437 22.25442332539336 40-41 22.81314372060004 26.25235035045943 28.514390900728344 22.42011502821219 42-43 22.578744705552307 26.606013735745687 27.909165982881174 22.906075575820832 44-45 21.914420519338776 26.891212869656723 28.397041428858284 22.797325182146217 46-47 21.88341008754595 26.42772576461584 28.042486526693427 23.646377621144783 48-49 22.19784207267686 26.495449862260383 28.13631771166613 23.170390353396623 50-51 22.100275479982162 26.728328800949697 27.970741245026442 23.2006544740417 52-53 21.75653795330695 26.931739384360604 27.800106330157575 23.51161633217487 54-55 21.583388623151784 26.485966765429854 28.3142433758007 23.616401235617662 56-57 22.116522963878293 26.45318195647889 27.694462210087917 23.7358328695549 58-59 21.538728510746267 26.680927932649627 28.283106794984793 23.49723676161931 60-61 22.15913545326592 26.89910561102742 27.6565340371567 23.285224898549963 62-63 22.28199581677091 26.684090201560846 27.421051121032985 23.612862860635257 64-65 21.636174308646964 26.403001531731057 27.6209365270228 24.33988763259918 66-67 21.71966870164003 26.22593787161386 27.50227885229068 24.552114574455434 68-69 21.256330928197993 26.362601060714407 27.8501253155854 24.530942695502198 70-71 21.496279769511524 26.145170137965934 27.789993156654468 24.568556935868077 72-73 21.490300851521422 26.498364590579808 27.25339348103591 24.757941076862867 74-75 21.556165926413104 26.42645528710497 27.112186196446775 24.905192590035146 76-77 21.269328710649482 26.190141652343556 27.287577882716086 25.252951754290876 78-79 21.181503314494027 26.13617995256779 27.491024372292905 25.191292360645278 80-81 21.17274690266702 26.339966199518322 27.21876435281035 25.268522545004306 82-83 21.598149225963397 25.98839928304945 27.37696293081705 25.0364885601701 84-85 21.733536379918675 26.19750621381895 26.860139184592484 25.208818221669894 86-87 21.077830833768264 26.314683262339457 27.205019659611978 25.402466244280298 88-89 21.127852780103392 25.828875570072746 27.227604217881318 25.81566743194254 90-91 21.560400838802988 25.498384169835685 27.273679060387963 25.66753593097337 92-93 21.497797743782865 25.63923723259351 27.13302633703993 25.729938686583697 94-95 20.925952915901362 25.488305540821955 27.351046306428074 26.23469523684861 96-97 20.83465479138101 25.305103623001862 27.520079542560023 26.340162043057102 98-99 20.80180610513106 25.442411274598342 27.308656295280592 26.447126324990005 100 20.667907459697847 25.52875102275995 27.409623233880115 26.39371828366209 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1105.0 1 827.0 2 1114.5 3 1736.5 4 1416.5 5 1131.5 6 1625.5 7 2857.5 8 4454.5 9 5451.5 10 5366.0 11 5193.5 12 5807.5 13 6784.0 14 7807.5 15 9012.0 16 10142.0 17 11285.0 18 12785.0 19 14009.0 20 15254.5 21 17337.5 22 20025.5 23 23880.5 24 29338.0 25 36175.5 26 45254.5 27 56931.5 28 72099.0 29 92637.0 30 117373.5 31 140754.5 32 168566.0 33 197630.0 34 223022.5 35 249163.5 36 277907.0 37 315527.5 38 339588.5 39 345095.0 40 349998.5 41 356297.5 42 368907.5 43 389161.0 44 414439.5 45 449998.0 46 483533.5 47 509845.5 48 540688.5 49 560927.0 50 570922.0 51 578938.0 52 573195.0 53 548411.0 54 506615.0 55 451097.0 56 396219.0 57 342722.0 58 288372.5 59 236568.0 60 183634.0 61 138570.0 62 106120.5 63 81168.5 64 59108.0 65 40834.5 66 29018.5 67 21741.5 68 15978.5 69 11368.0 70 8389.0 71 6189.0 72 4445.5 73 3208.0 74 2337.5 75 1570.5 76 989.5 77 606.5 78 366.5 79 222.5 80 132.0 81 85.0 82 66.0 83 46.5 84 26.5 85 21.0 86 20.5 87 10.5 88 6.5 89 8.0 90 5.0 91 1.0 92 0.5 93 0.0 94 1.0 95 1.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.050654335057070954 2 0.0 3 0.0 4 0.014944825094852137 5 0.0037920897008839557 6 3.193338695481226E-5 7 2.075670152062797E-4 8 3.353005630255287E-4 9 0.002067686805324094 10-11 7.304762265913304E-4 12-13 2.674421157465527E-4 14-15 3.193338695481226E-5 16-17 8.382514075638218E-5 18-19 0.0 20-21 2.3950040216109197E-5 22-23 7.983346738703066E-6 24-25 7.983346738703066E-5 26-27 2.3950040216109197E-5 28-29 7.983346738703065E-4 30-31 7.823679803929004E-4 32-33 0.01387904830523528 34-35 0.0227884632656279 36-37 0.030476426174998954 38-39 0.020134000475009132 40-41 1.3970856792730363E-4 42-43 0.0017323862422985654 44-45 0.008861514879960402 46-47 0.013420005867759854 48-49 0.005029508445382931 50-51 0.009647874533722654 52-53 0.014980750155176302 54-55 0.009300598950589071 56-57 0.0026983711976816364 58-59 0.008035238492504634 60-61 0.0016365860814341283 62-63 8.422430809331734E-4 64-65 0.018649097981610362 66-67 0.044052107304163514 68-69 0.05334871458138324 70-71 0.04696602886379014 72-73 0.03910642399953697 74-75 0.027554521268633628 76-77 0.027778054977317314 78-79 0.011683627952091937 80-81 0.0017403695890372683 82-83 0.026460802765431307 84-85 0.04626748602415361 86-87 0.046810353602385425 88-89 0.058102797564280904 90-91 0.03339833108136427 92-93 0.02492800019160032 94-95 0.006773869707789551 96-97 0.0036204477460018403 98-99 0.0221378205064236 100 0.01887263169029405 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.2526075E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.641215241452215 #Duplication Level Percentage of deduplicated Percentage of total 1 73.24237885551025 41.48537345550945 2 15.290889631966534 17.32189141755013 3 5.315661216408262 9.032565333276603 4 2.2879477507443564 5.183685640444303 5 1.1485946557649456 3.2528898561181996 6 0.6830241302657518 2.3212390066492885 7 0.4311983629239427 1.7096519500295808 8 0.30323771462597826 1.3740602130764878 9 0.21255031601891003 1.0835197379339194 >10 1.008854714845927 9.90750821876228 >50 0.0489546008822061 1.8846611411195393 >100 0.02343517691584464 2.41271894650277 >500 0.0018897396992152659 0.7204715682707424 >1k 0.001270987635242958 1.33521773666931 >5k 3.7381930933172346E-5 0.14903024255739294 >10k+ 7.476386186634469E-5 0.8255155355299618 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 29188 0.23301792460926504 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22781 0.18186862205439455 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15195 0.12130695369459309 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 7.983346738703066E-6 2 0.0 0.0 0.0 0.0 7.983346738703066E-6 3 0.0 0.0 0.0 0.0 7.983346738703066E-6 4 0.0 0.0 0.0 1.596669347740613E-5 7.983346738703066E-6 5 0.0 0.0 0.0 1.596669347740613E-5 7.983346738703066E-6 6 0.0 0.0 0.0 1.596669347740613E-5 1.596669347740613E-5 7 0.0 0.0 0.0 1.596669347740613E-5 1.596669347740613E-5 8 0.0 0.0 0.0 1.596669347740613E-5 3.991673369351533E-5 9 0.0 0.0 0.0 2.3950040216109197E-5 3.991673369351533E-5 10-11 0.0 0.0 0.0 2.794171358546073E-5 3.991673369351533E-5 12-13 0.0 0.0 0.0 3.5925060324163795E-5 6.386677390962452E-5 14-15 0.0 0.0 0.0 5.588342717092146E-5 6.785844727897605E-5 16-17 0.0 0.0 0.0 1.1575852771119444E-4 7.584179401767913E-5 18-19 0.0 0.0 0.0 1.9160032172887357E-4 8.382514075638218E-5 20-21 0.0 0.0 0.0 4.2311737715126246E-4 1.1176685434184292E-4 22-23 0.0 0.0 0.0 0.0012174603776522175 1.1575852771119444E-4 24-25 0.0 0.0 0.0 0.003069596821031329 1.1975020108054598E-4 26-27 0.0 0.0 0.0 0.006238985476296446 1.2773354781924905E-4 28-29 0.0 0.0 0.0 0.018146147137072067 1.4370024129665518E-4 30-31 0.0 0.0 0.0 0.061739212003760154 1.596669347740613E-4 32-33 0.0 0.0 0.0 0.16233337258478814 1.596669347740613E-4 34-35 0.0 0.0 0.0 0.3048520785641152 1.6765028151276436E-4 36-37 0.0 0.0 0.0 0.5251006400648248 1.6765028151276436E-4 38-39 0.0 0.0 0.0 0.8857243789455196 1.8361697499017052E-4 40-41 0.0 0.0 0.0 1.3155797007442476 1.9160032172887357E-4 42-43 0.0 0.0 0.0 1.726175198535854 1.9958366846757662E-4 44-45 0.0 0.0 0.0 2.166125462285672 2.1954203531433428E-4 46-47 0.0 0.0 0.0 2.6630289216693974 2.434920755304435E-4 48-49 0.0 0.0 0.0 3.198188578624988 2.6345044237720115E-4 50-51 0.0 0.0 0.0 3.77169224996657 2.6345044237720115E-4 52-53 0.0 0.0 0.0 4.363789135862591 2.834088092239588E-4 54-55 0.0 0.0 0.0 4.968583534746519 3.193338695481226E-4 56-57 0.0 0.0 0.0 5.611749889730023 3.392922363948803E-4 58-59 0.0 0.0 0.0 6.341615390295843 3.512672565029349E-4 60-61 0.0 0.0 0.0 7.081184648822556 3.512672565029349E-4 62-63 0.0 0.0 0.0 7.8575411691212125 3.752172967190441E-4 64-65 0.0 0.0 0.0 8.58064876667272 3.752172967190441E-4 66-67 0.0 0.0 0.0 9.353907748436761 3.792089700883956E-4 68-69 0.0 0.0 0.0 10.183389449608118 3.8320064345774714E-4 70-71 0.0 0.0 0.0 11.020850505844809 3.8320064345774714E-4 72-73 0.0 0.0 0.0 11.812958169258927 4.111423570432079E-4 74-75 0.0 0.0 0.0 12.578349562811974 4.2311737715126246E-4 76-77 0.0 0.0 0.0 13.41423789974114 4.311007238899655E-4 78-79 0.0 3.991673369351533E-6 0.0 14.280802246513773 4.3908407062866856E-4 80-81 0.0 7.983346738703066E-6 0.0 15.123775803673537 4.6303411084477777E-4 82-83 0.0 7.983346738703066E-6 0.0 15.992902804749292 5.189175380156993E-4 84-85 0.0 7.983346738703066E-6 0.0 16.833968342038506 5.189175380156993E-4 86-87 0.0 7.983346738703066E-6 0.0 17.67111006440565 5.308925581237538E-4 88 0.0 7.983346738703066E-6 0.0 18.302037948838723 5.428675782318084E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 17445 0.0 49.591335 1 TACCTGG 11165 0.0 42.21671 2 GTATCAA 31805 0.0 41.407276 1 ACCTGGG 13370 0.0 34.41097 3 ATCAACG 39850 0.0 32.288715 3 CAACGCA 39945 0.0 32.159725 5 TATCAAC 41265 0.0 31.670965 2 TCAACGC 41680 0.0 30.995441 4 AACGCAG 42925 0.0 30.407751 6 GTACATG 44335 0.0 27.265554 1 TGGGGGG 26730 0.0 27.017012 6 ACGCAGA 48170 0.0 26.745546 7 TACATGG 44495 0.0 26.742046 2 CCTGGGG 12835 0.0 26.692081 4 CGCAGAG 49300 0.0 26.075228 8 ACATGGG 44075 0.0 25.728271 3 CATGGGG 34650 0.0 25.348768 4 ATGGGGG 27255 0.0 24.79071 5 GTACCTG 19295 0.0 24.781929 1 TATCACG 930 0.0 24.760263 2 >>END_MODULE