FastQCFastQC Report
Mon 6 Jun 2016
ERR1211418_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211418_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11945981
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT410580.34369718150397194No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT294960.2469114926601675No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT212840.178168707952909No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG129550.053.5559122
GGTATCA210550.047.7311671
ACCTGGG147800.045.7664953
GTATCAA389950.042.2163281
CCTGGGG137650.034.5589184
GTACCTG207050.033.8605651
ATCAACG485150.032.9721453
CAACGCA484700.032.9247555
TATCAAC501600.032.3280142
GTACATG518700.031.946451
TCAACGC511900.031.313034
AACGCAG524500.030.9108416
TACATGG528250.030.7683662
ACATGGG528300.029.6740283
TATCACG10300.029.2475932
CATGGGG373850.028.2905224
GTACTAG52450.028.0269321
ACGCAGA572500.027.8929277
CGCAGAG579400.027.4782418
TGGGGGG302800.027.3845626
Produced by FastQC (version 0.11.5)