##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211418_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 11945981 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.10652051095678 31.0 31.0 34.0 28.0 34.0 2 31.150724331471814 31.0 31.0 34.0 28.0 34.0 3 31.2112543959345 31.0 31.0 34.0 27.0 34.0 4 34.83856043300253 37.0 35.0 37.0 32.0 37.0 5 34.896328815523816 37.0 35.0 37.0 32.0 37.0 6 34.95565504415251 37.0 35.0 37.0 32.0 37.0 7 34.87664780313982 37.0 35.0 37.0 32.0 37.0 8 34.8772288353715 37.0 35.0 37.0 32.0 37.0 9 36.40811859653887 39.0 37.0 39.0 32.0 39.0 10-11 36.31593474826387 39.0 36.0 39.0 31.5 39.0 12-13 36.20744022613128 39.0 35.0 39.0 31.0 39.0 14-15 37.33552966474666 40.0 37.0 41.0 31.0 41.0 16-17 37.336091108800524 40.0 37.0 41.0 31.0 41.0 18-19 37.345164076520796 40.0 37.0 41.0 31.0 41.0 20-21 37.329443224461855 40.0 37.0 41.0 31.0 41.0 22-23 37.19680727769448 40.0 36.0 41.0 31.0 41.0 24-25 37.09791937556238 40.0 36.0 41.0 30.0 41.0 26-27 36.955343014525134 39.5 36.0 41.0 30.0 41.0 28-29 36.82712771768179 39.0 36.0 41.0 30.0 41.0 30-31 36.664303961307155 39.0 35.5 41.0 30.0 41.0 32-33 36.45927010933636 39.0 35.0 41.0 29.5 41.0 34-35 36.335420004434965 39.0 35.0 40.0 29.0 41.0 36-37 36.20434776348631 39.0 35.0 40.0 28.5 41.0 38-39 36.05190938274554 39.0 35.0 40.0 28.0 41.0 40-41 35.8882958209962 38.0 35.0 40.0 27.0 41.0 42-43 35.69386494922435 38.0 34.5 40.0 27.0 41.0 44-45 35.545491701351274 38.0 34.0 40.0 26.0 41.0 46-47 35.30972550517199 38.0 34.0 40.0 25.5 41.0 48-49 35.062597705454245 38.0 33.5 40.0 24.0 41.0 50-51 34.40171368094424 37.5 32.5 39.5 24.0 40.5 52-53 34.71199510529943 38.0 33.5 39.5 24.5 40.5 54-55 35.1257602452239 38.0 34.0 40.0 25.0 41.0 56-57 35.04311600696502 38.0 34.0 40.0 25.0 41.0 58-59 34.838073951398385 38.0 33.5 40.0 25.0 41.0 60-61 34.61637901483353 37.0 33.0 40.0 25.0 41.0 62-63 34.333607135320236 37.0 33.0 40.0 24.0 41.0 64-65 34.13252544935405 36.0 33.0 40.0 24.0 41.0 66-67 33.94416720569035 36.0 33.0 39.5 23.5 41.0 68-69 33.6243817899928 35.5 32.0 39.0 23.0 41.0 70-71 33.287472037666895 35.0 32.0 39.0 23.0 41.0 72-73 32.92964684105893 35.0 32.0 38.0 22.0 40.0 74-75 32.57916566249352 35.0 31.0 37.0 21.5 39.5 76-77 32.23133767750007 35.0 31.0 37.0 20.5 39.0 78-79 31.875932332388608 35.0 31.0 36.0 20.0 39.0 80-81 31.52745676558501 35.0 30.5 36.0 20.0 38.5 82-83 31.202805780454533 34.5 30.0 35.5 19.0 37.0 84-85 30.89992550632719 34.0 30.0 35.0 18.5 37.0 86-87 30.604409215115947 34.0 29.5 35.0 18.0 36.5 88-89 30.339312359529117 34.0 29.0 35.0 17.5 36.0 90-91 30.077430099713034 34.0 29.0 35.0 16.5 36.0 92-93 29.8269495824579 34.0 29.0 35.0 15.0 35.5 94-95 29.59170841641218 34.0 29.0 35.0 14.0 35.0 96-97 29.34133881512117 34.0 27.5 35.0 10.5 35.0 98-99 29.079268333006723 34.0 27.0 35.0 10.0 35.0 100 27.865795785210107 32.0 25.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.2912642792768665 1101 2 -0.2892221948608622 1101 3 -0.31007810757123266 1101 4 -0.24258421954812093 1101 5 -0.20223975516238824 1101 6 -0.22629265667928422 1101 7 -0.3269434402841256 1101 8 -0.24767250990889522 1101 9 -0.40276128165435665 1101 10-11 -0.4214149412470434 1101 12-13 -0.42232712949518003 1101 14-15 -0.6168814031056442 1101 16-17 -0.6356460625565745 1101 18-19 -0.5803166895191723 1101 20-21 -0.6977818707566286 1101 22-23 -0.5908264032384665 1101 24-25 -0.6408680474342816 1101 26-27 -0.6663690260327542 1101 28-29 -0.6937353912501152 1101 30-31 -0.7418014178373156 1101 32-33 -0.6950215045691195 1101 34-35 -0.7708419118366052 1101 36-37 -0.7204300182929018 1101 38-39 -0.8228889952672986 1101 40-41 -0.985322683668123 1101 42-43 -1.0225816012339308 1101 44-45 -0.9906017599534565 1101 46-47 -1.036766155013403 1101 48-49 -1.0667706367857264 1101 50-51 -0.939083237694625 1101 52-53 -0.7004962931751564 1101 54-55 -0.7430996057837618 1101 56-57 -0.8016519629039749 1101 58-59 -0.7653067890940619 1101 60-61 -0.823434179939845 1101 62-63 -0.771408326068098 1101 64-65 -0.7412485533264643 1101 66-67 -0.7791728866241172 1101 68-69 -0.8360684451620912 1101 70-71 -0.8076792582689762 1101 72-73 -0.8931812184054806 1101 74-75 -0.8850432582368342 1101 76-77 -0.8965612753168202 1101 78-79 -1.0110492453362703 1101 80-81 -1.0908613001948275 1101 82-83 -1.0326007489762432 1101 84-85 -1.027433175365939 1101 86-87 -1.1956325656569895 1101 88-89 -1.305811773810806 1101 90-91 -1.2790022826203327 1101 92-93 -1.3717433724163577 1101 94-95 -1.5188734486236548 1101 96-97 -1.5565419148978172 1101 98-99 -1.6196002356485373 1101 100 -1.6580402209434517 1102 1 -0.4056166106188428 1102 2 -0.3720110311472844 1102 3 -0.49345946907507 1102 4 -0.41075038938775776 1102 5 -0.30683901405622294 1102 6 -0.3173921728237872 1102 7 -0.3545639011801569 1102 8 -0.3565961046693289 1102 9 -0.45261284474803887 1102 10-11 -0.43208960312280453 1102 12-13 -0.5145268856909908 1102 14-15 -0.6798433584256216 1102 16-17 -0.6949214022535912 1102 18-19 -0.7507402261957665 1102 20-21 -0.7179809826968011 1102 22-23 -0.698961671464339 1102 24-25 -0.7779789071342549 1102 26-27 -0.7167770280079182 1102 28-29 -0.7739860378026506 1102 30-31 -0.7848728802668816 1102 32-33 -0.8317350427904628 1102 34-35 -0.8854459998253716 1102 36-37 -0.8807059591498501 1102 38-39 -0.9475182339080916 1102 40-41 -1.102800880233481 1102 42-43 -0.9808942723710246 1102 44-45 -1.053654116031673 1102 46-47 -1.141358498932938 1102 48-49 -1.209605659650208 1102 50-51 -1.0841708243843655 1102 52-53 -0.8184967089884694 1102 54-55 -0.8443548298254271 1102 56-57 -0.8399547451898641 1102 58-59 -0.8162749818473785 1102 60-61 -0.9279208284043108 1102 62-63 -0.8096441095038287 1102 64-65 -0.9568096552656229 1102 66-67 -0.9797054233446261 1102 68-69 -0.8854851386127294 1102 70-71 -0.9986506483584776 1102 72-73 -0.9584185944361998 1102 74-75 -1.0331735747376918 1102 76-77 -1.0504653861030988 1102 78-79 -1.1378809446529026 1102 80-81 -1.1180175478200844 1102 82-83 -1.1965025597671293 1102 84-85 -1.2560044145698193 1102 86-87 -1.3662597947994435 1102 88-89 -1.392691301586094 1102 90-91 -1.38260405768888 1102 92-93 -1.4581344139498817 1102 94-95 -1.4450387741064823 1102 96-97 -1.5099233285681066 1102 98-99 -1.6418898353169773 1102 100 -1.671003281880715 1103 1 -0.42626703168862434 1103 2 -0.47092668590765996 1103 3 -0.49024545158243527 1103 4 -0.30467744815320685 1103 5 -0.2812134539238329 1103 6 -0.2955990492015772 1103 7 -0.38140811223865256 1103 8 -0.46587093228915677 1103 9 -0.5519874565526024 1103 10-11 -0.5280508986242012 1103 12-13 -0.643269308429403 1103 14-15 -0.7412781803356197 1103 16-17 -0.7874431180820025 1103 18-19 -0.7910364884697714 1103 20-21 -0.7944093756930144 1103 22-23 -0.7576999392507204 1103 24-25 -0.744630177801838 1103 26-27 -0.7862261561423693 1103 28-29 -0.8407401019337541 1103 30-31 -0.8698922273771856 1103 32-33 -0.9516751388092288 1103 34-35 -0.8733946664428203 1103 36-37 -0.9732269394701731 1103 38-39 -0.9119035856590116 1103 40-41 -1.0058611679397913 1103 42-43 -1.0374084496809743 1103 44-45 -1.0229547966651182 1103 46-47 -1.0667467106067434 1103 48-49 -1.0241422194454444 1103 50-51 -0.9592861367685899 1103 52-53 -0.7786905673316795 1103 54-55 -0.79133284489658 1103 56-57 -0.8340314072547343 1103 58-59 -0.7801906734786783 1103 60-61 -0.800252008666682 1103 62-63 -0.7957157308356884 1103 64-65 -0.7441329747614631 1103 66-67 -0.825887686488862 1103 68-69 -0.8208530821672753 1103 70-71 -0.8347812956468488 1103 72-73 -0.8006996415123808 1103 74-75 -0.8882877047687963 1103 76-77 -0.9980265969962261 1103 78-79 -1.0189593028567252 1103 80-81 -0.9173121497180254 1103 82-83 -0.9762155914496873 1103 84-85 -1.0566242109934088 1103 86-87 -1.1582344578641433 1103 88-89 -1.1604690047941766 1103 90-91 -1.1574270304451986 1103 92-93 -1.233653549883389 1103 94-95 -1.2558289518373336 1103 96-97 -1.410403009746556 1103 98-99 -1.408082432636352 1103 100 -1.4079129096012934 1104 1 -0.4634729756916265 1104 2 -0.43308068290697577 1104 3 -0.42736667111207893 1104 4 -0.22920831242130646 1104 5 -0.38680546330320453 1104 6 -0.38164559239132245 1104 7 -0.31930295761737426 1104 8 -0.4568454629272338 1104 9 -0.5155183063822619 1104 10-11 -0.485331376036477 1104 12-13 -0.5688489867133413 1104 14-15 -0.7159081899085962 1104 16-17 -0.7580077814273949 1104 18-19 -0.8318338565671084 1104 20-21 -0.7483695177083902 1104 22-23 -0.7130471782549535 1104 24-25 -0.7269054313253491 1104 26-27 -0.7661147419564642 1104 28-29 -0.8697191739711982 1104 30-31 -0.8990566378683909 1104 32-33 -0.9288859449273446 1104 34-35 -0.8288052846878529 1104 36-37 -0.9367614553451062 1104 38-39 -0.921740930544324 1104 40-41 -0.8373685849859669 1104 42-43 -0.9159947009041716 1104 44-45 -0.9310785146046143 1104 46-47 -0.9360429863986681 1104 48-49 -0.9723604270832169 1104 50-51 -0.9812086044078612 1104 52-53 -0.8301024773971548 1104 54-55 -0.7645894103389494 1104 56-57 -0.6873326598024434 1104 58-59 -0.7345805780351782 1104 60-61 -0.741046063778775 1104 62-63 -0.8691103906050657 1104 64-65 -0.801533297541404 1104 66-67 -0.7196420510354002 1104 68-69 -0.7887415008602758 1104 70-71 -0.7616143776529185 1104 72-73 -0.7223586423971184 1104 74-75 -0.8010867501089365 1104 76-77 -0.8562463188190605 1104 78-79 -0.8480589182551661 1104 80-81 -0.864849561010228 1104 82-83 -0.9032824786533524 1104 84-85 -0.9179433302926725 1104 86-87 -0.9338701756231238 1104 88-89 -0.9455232307958106 1104 90-91 -0.9342471517170736 1104 92-93 -1.0260929428184404 1104 94-95 -1.0793067545336825 1104 96-97 -1.1776529830435685 1104 98-99 -1.2511069837352018 1104 100 -1.224546174170996 1105 1 -0.46273880427923686 1105 2 -0.48923059032493654 1105 3 -0.476610007093484 1105 4 -0.4124026259321951 1105 5 -0.394243538017065 1105 6 -0.4095795361691472 1105 7 -0.42023651165541054 1105 8 -0.4516711171322285 1105 9 -0.5581684387693429 1105 10-11 -0.5890121671354791 1105 12-13 -0.5761773994759807 1105 14-15 -0.783852559473381 1105 16-17 -0.725444404144163 1105 18-19 -0.6820886084235198 1105 20-21 -0.7525635975304183 1105 22-23 -0.7112328751621035 1105 24-25 -0.7747944441468917 1105 26-27 -0.8199639143374995 1105 28-29 -0.8183084567427628 1105 30-31 -0.815399011548628 1105 32-33 -0.960234704251377 1105 34-35 -0.9071623938994406 1105 36-37 -0.8817344902692099 1105 38-39 -0.9917369866411931 1105 40-41 -0.854835408122689 1105 42-43 -0.8193503901986716 1105 44-45 -0.9275173453301377 1105 46-47 -0.9398699245359623 1105 48-49 -1.010346268561399 1105 50-51 -0.8875322369049883 1105 52-53 -0.7788993199666194 1105 54-55 -0.7060789139113908 1105 56-57 -0.6821274181618406 1105 58-59 -0.6677228422730721 1105 60-61 -0.6737328694269422 1105 62-63 -0.8577377181118351 1105 64-65 -0.833850136741809 1105 66-67 -0.7461883730367731 1105 68-69 -0.7216155676281133 1105 70-71 -0.7012827487511686 1105 72-73 -0.7076405375995307 1105 74-75 -0.7231508363172594 1105 76-77 -0.7341416481724359 1105 78-79 -0.8211576923381578 1105 80-81 -0.7821299540932252 1105 82-83 -0.7653283985350683 1105 84-85 -0.8240428720997457 1105 86-87 -0.8947066620192388 1105 88-89 -0.8420531472108124 1105 90-91 -0.8545593422876436 1105 92-93 -0.8969291118593183 1105 94-95 -1.0049311531142884 1105 96-97 -0.9754649327987863 1105 98-99 -1.0449852313676224 1105 100 -1.0584383527079027 1106 1 -0.4523711043237455 1106 2 -0.5246231654260889 1106 3 -0.6081820997958758 1106 4 -0.3902476304632714 1106 5 -0.42281787241196866 1106 6 -0.43933731521184427 1106 7 -0.4959835128161103 1106 8 -0.4770912384968682 1106 9 -0.7220998571386801 1106 10-11 -0.7623970568109755 1106 12-13 -0.6453711221098146 1106 14-15 -0.8298942467352433 1106 16-17 -0.8056853072840013 1106 18-19 -0.7855878759084476 1106 20-21 -0.9150889946044245 1106 22-23 -0.8755577119697548 1106 24-25 -0.868895255285409 1106 26-27 -0.8978814070812504 1106 28-29 -0.8964030007803885 1106 30-31 -0.9544716187527129 1106 32-33 -0.9581319024410604 1106 34-35 -0.9029736776516941 1106 36-37 -0.8346940797307028 1106 38-39 -1.0293962201681808 1106 40-41 -0.9903902802432327 1106 42-43 -0.9703874060987872 1106 44-45 -0.9489597456492476 1106 46-47 -0.9346043319166668 1106 48-49 -0.9327981112861181 1106 50-51 -0.9672695844132093 1106 52-53 -0.8224190453918752 1106 54-55 -0.8198459186665943 1106 56-57 -0.7960130512448202 1106 58-59 -0.7441499800048845 1106 60-61 -0.7759881320736568 1106 62-63 -0.8528268015825731 1106 64-65 -0.7686468883588802 1106 66-67 -0.8341777375813919 1106 68-69 -0.7682251789321981 1106 70-71 -0.7815758488503377 1106 72-73 -0.730685720007088 1106 74-75 -0.7444621232877324 1106 76-77 -0.7254935514820957 1106 78-79 -0.72803366698259 1106 80-81 -0.7774983991890885 1106 82-83 -0.7456932400561698 1106 84-85 -0.7362927240206325 1106 86-87 -0.7500463725879065 1106 88-89 -0.7615946432777427 1106 90-91 -0.8644564155445984 1106 92-93 -0.860344724753368 1106 94-95 -0.8571008731511078 1106 96-97 -0.9772351899308056 1106 98-99 -0.9887311353648265 1106 100 -1.0344931059195552 1107 1 -0.5683870659385271 1107 2 -0.5298954172411605 1107 3 -0.5689779678379345 1107 4 -0.42334838531775887 1107 5 -0.37210800139799716 1107 6 -0.44422770550927027 1107 7 -0.49576544670316025 1107 8 -0.47319361202042387 1107 9 -0.6104501659012627 1107 10-11 -0.5960597942064609 1107 12-13 -0.7092100321839823 1107 14-15 -0.8017636864458453 1107 16-17 -0.8419342072792304 1107 18-19 -0.8467232895406909 1107 20-21 -0.8441943056608991 1107 22-23 -0.8754260634749542 1107 24-25 -0.8646716873723648 1107 26-27 -0.8979325059187744 1107 28-29 -0.8480634529064517 1107 30-31 -0.9195682580414797 1107 32-33 -0.9825891033742451 1107 34-35 -1.009100586864129 1107 36-37 -0.9687539575451609 1107 38-39 -0.9301028051240721 1107 40-41 -0.9304010221006607 1107 42-43 -0.929566872955526 1107 44-45 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0.6516728528726574 2311 8 0.6235515806848824 2311 9 0.7980216945601413 2311 10-11 0.7556301481149674 2311 12-13 0.8667304642791791 2311 14-15 1.1140458562010167 2311 16-17 1.089593330662474 2311 18-19 1.1285703626032912 2311 20-21 1.1575726547627383 2311 22-23 1.159297246174809 2311 24-25 1.2073989655109685 2311 26-27 1.2396501295787772 2311 28-29 1.1967192977539298 2311 30-31 1.2316192979456417 2311 32-33 1.315460162677553 2311 34-35 1.263627976980871 2311 36-37 1.2604476299731076 2311 38-39 1.2988988417318268 2311 40-41 1.3151049028190513 2311 42-43 1.3560348927001868 2311 44-45 1.3686412236489574 2311 46-47 1.3933961011118114 2311 48-49 1.4294338373432964 2311 50-51 1.4027229787385593 2311 52-53 1.2367103222441358 2311 54-55 1.1959845961798194 2311 56-57 1.1531853325104109 2311 58-59 1.1598004060974674 2311 60-61 1.1909587543873101 2311 62-63 1.1810672013384789 2311 64-65 1.1491993923285193 2311 66-67 1.1586305774979877 2311 68-69 1.2068135396866282 2311 70-71 1.1934044373895532 2311 72-73 1.1678546583231864 2311 74-75 1.2038959980693775 2311 76-77 1.1852242845904115 2311 78-79 1.1779516946132027 2311 80-81 1.1941531192524941 2311 82-83 1.216570073521602 2311 84-85 1.2282328734950276 2311 86-87 1.2735194820831488 2311 88-89 1.297595853713176 2311 90-91 1.3341020697026273 2311 92-93 1.415057726444914 2311 94-95 1.4885896491082065 2311 96-97 1.5135721989767852 2311 98-99 1.5261410676726008 2311 100 1.614954448799125 2312 1 0.6662164495710563 2312 2 0.729193634676939 2312 3 0.7821882558298618 2312 4 0.6218739301673821 2312 5 0.6971723661342892 2312 6 0.5026815231380439 2312 7 0.6850871959740275 2312 8 0.7153981696470595 2312 9 0.9443373398623436 2312 10-11 0.968636111641807 2312 12-13 1.0088739243426303 2312 14-15 1.2044106102934435 2312 16-17 1.2162523575539765 2312 18-19 1.2074502625524772 2312 20-21 1.196282473630795 2312 22-23 1.224846719259432 2312 24-25 1.255944892583912 2312 26-27 1.3050180469454205 2312 28-29 1.3328926972906672 2312 30-31 1.275554968103819 2312 32-33 1.3586121519265575 2312 34-35 1.391843357246863 2312 36-37 1.3844447797070671 2312 38-39 1.406121666161603 2312 40-41 1.4204219038244617 2312 42-43 1.4488301070443939 2312 44-45 1.434315595527515 2312 46-47 1.4113821313940846 2312 48-49 1.5236676239469986 2312 50-51 1.4573060002990914 2312 52-53 1.3105723408967478 2312 54-55 1.274670269844428 2312 56-57 1.2416486306182222 2312 58-59 1.2676313927680027 2312 60-61 1.1789178627727424 2312 62-63 1.2151760247596926 2312 64-65 1.2144178359416955 2312 66-67 1.2045493073806313 2312 68-69 1.261298807962973 2312 70-71 1.2167165704216671 2312 72-73 1.216530111180525 2312 74-75 1.232127979597756 2312 76-77 1.2169119965765844 2312 78-79 1.1974409652113316 2312 80-81 1.1141527563806441 2312 82-83 1.2584933197861545 2312 84-85 1.2744771099095793 2312 86-87 1.3942182814265465 2312 88-89 1.3904606476116967 2312 90-91 1.3956088284279353 2312 92-93 1.4404586237216854 2312 94-95 1.4966627217158681 2312 96-97 1.5567306295754975 2312 98-99 1.4859236585187148 2312 100 1.621356950748126 2313 1 0.6227912921253704 2313 2 0.6749279511879891 2313 3 0.6924492278852483 2313 4 0.6311362617707701 2313 5 0.5963945855872126 2313 6 0.6415445742780221 2313 7 0.6587519244177855 2313 8 0.6969724324719664 2313 9 0.8349998262488043 2313 10-11 0.761420831931801 2313 12-13 0.862145339618472 2313 14-15 1.045846643945758 2313 16-17 1.0447814217766904 2313 18-19 1.079381955339791 2313 20-21 1.1009321750053473 2313 22-23 1.1415419461611833 2313 24-25 1.166177405419532 2313 26-27 1.1635229084877636 2313 28-29 1.1833420302066529 2313 30-31 1.1951382305170029 2313 32-33 1.2546981486077655 2313 34-35 1.2238654402216724 2313 36-37 1.2919231787133185 2313 38-39 1.295105521774218 2313 40-41 1.2932839674583434 2313 42-43 1.404730332306599 2313 44-45 1.3400880551293497 2313 46-47 1.3219037624066274 2313 48-49 1.366888687075047 2313 50-51 1.3376573770290605 2313 52-53 1.1573059458361357 2313 54-55 1.1886917098098877 2313 56-57 1.1507264742392707 2313 58-59 1.1749319284261475 2313 60-61 1.1913278663725322 2313 62-63 1.1774668736395526 2313 64-65 1.1593764483865314 2313 66-67 1.1306250981633283 2313 68-69 1.1717465795269817 2313 70-71 1.184286918700721 2313 72-73 1.203323227253243 2313 74-75 1.1949520317071531 2313 76-77 1.1845515354251646 2313 78-79 1.2196468264003393 2313 80-81 1.209952036093096 2313 82-83 1.2147587179092767 2313 84-85 1.2628056983313947 2313 86-87 1.3349587156720126 2313 88-89 1.3978089088221104 2313 90-91 1.4171095952623638 2313 92-93 1.5064162027255499 2313 94-95 1.5166440727101893 2313 96-97 1.5547059178370155 2313 98-99 1.6005959318413865 2313 100 1.7938427998070061 2314 1 0.6234551165482536 2314 2 0.5825756886674647 2314 3 0.7024612593887873 2314 4 0.5805577264250488 2314 5 0.5859870863332759 2314 6 0.5979014825927536 2314 7 0.6565389740360033 2314 8 0.6513123912489647 2314 9 0.7892417348176579 2314 10-11 0.7678689595618664 2314 12-13 0.5556647011775766 2314 14-15 0.9517778010354689 2314 16-17 0.9942886963657713 2314 18-19 0.9930187900476781 2314 20-21 0.9911902275156024 2314 22-23 0.9400460491515403 2314 24-25 0.9896999585534516 2314 26-27 1.0859833562955146 2314 28-29 1.1415174879982075 2314 30-31 1.0520656631165153 2314 32-33 1.103388953580243 2314 34-35 1.1780110932534882 2314 36-37 1.2559362061194506 2314 38-39 1.3187055961651097 2314 40-41 1.3070938217370625 2314 42-43 1.4268638925078534 2314 44-45 1.4023967575919514 2314 46-47 1.3883555022331109 2314 48-49 1.4305611451746074 2314 50-51 1.4209163516304315 2314 52-53 1.2454595385593095 2314 54-55 1.1996207329005202 2314 56-57 1.2024790729905916 2314 58-59 1.1900127204178759 2314 60-61 1.1920541012535963 2314 62-63 1.2000340756917822 2314 64-65 1.1336082168976915 2314 66-67 1.1315214545888637 2314 68-69 1.2101866162202057 2314 70-71 1.2781545711566054 2314 72-73 1.241021277685192 2314 74-75 1.2539319298402987 2314 76-77 1.2411562207267934 2314 78-79 1.2508569131935197 2314 80-81 1.2973360966057363 2314 82-83 1.178733530911785 2314 84-85 1.1850290764872327 2314 86-87 1.4012836786988245 2314 88-89 1.4564153185916702 2314 90-91 1.4821370419001418 2314 92-93 1.625291956924226 2314 94-95 1.6477143989576426 2314 96-97 1.6693315281312842 2314 98-99 1.7025893737954867 2314 100 1.5949738674448035 2315 1 0.4425914337013168 2315 2 0.49907416997875487 2315 3 0.5344607024617005 2315 4 0.4377257416985856 2315 5 0.3840178236003027 2315 6 -0.280283236874574 2315 7 0.26521014962500544 2315 8 0.32450580501387094 2315 9 0.5764488184648968 2315 10-11 0.6646282086206199 2315 12-13 0.69550459179451 2315 14-15 0.8465610617587274 2315 16-17 0.7080262932256787 2315 18-19 0.7673944688530838 2315 20-21 0.8222972136090831 2315 22-23 0.761602793491484 2315 24-25 0.8519227904372784 2315 26-27 0.8977088124998716 2315 28-29 0.9931342379705015 2315 30-31 1.011549907514656 2315 32-33 1.0985840847568795 2315 34-35 1.087277786615381 2315 36-37 1.1052611668534311 2315 38-39 1.1541865791059323 2315 40-41 1.163768105875583 2315 42-43 1.227023365657658 2315 44-45 1.1850060432092562 2315 46-47 1.2418114220521872 2315 48-49 1.228385252960699 2315 50-51 1.2040211707959187 2315 52-53 1.0449443958708216 2315 54-55 0.8548897888451421 2315 56-57 0.8646880281512423 2315 58-59 0.8427420507931842 2315 60-61 0.7816718674431584 2315 62-63 0.9633274011667723 2315 64-65 0.9859747196455615 2315 66-67 0.9800752333413101 2315 68-69 1.0327836967131745 2315 70-71 1.0891660394795224 2315 72-73 1.1191960838801336 2315 74-75 1.1085526823915544 2315 76-77 1.0358533344626721 2315 78-79 1.0061248036086035 2315 80-81 1.0108394982123023 2315 82-83 1.1284337842609276 2315 84-85 1.0537390395429185 2315 86-87 1.157917591569774 2315 88-89 1.320129463873016 2315 90-91 1.3297517378461166 2315 92-93 1.4159573766926137 2315 94-95 1.4727204023174032 2315 96-97 1.4815141515686285 2315 98-99 1.581609883394453 2315 100 1.6282032493863206 2316 1 0.06638724534816731 2316 2 0.017346427689652444 2316 3 0.13421325482223523 2316 4 0.07647483217761675 2316 5 0.06852969931490804 2316 6 0.226545019713015 2316 7 0.15929568918376447 2316 8 0.19871240985276017 2316 9 0.34296132709906146 2316 10-11 0.23080691331859526 2316 12-13 0.311876477855229 2316 14-15 0.4770811643396158 2316 16-17 0.5024453075991886 2316 18-19 0.4224434888389297 2316 20-21 0.3885439934040349 2316 22-23 0.29257166539009205 2316 24-25 0.37529420679274494 2316 26-27 0.49999003069146397 2316 28-29 0.5653438821778565 2316 30-31 0.5481073157926986 2316 32-33 0.630830607429111 2316 34-35 0.6618718680245976 2316 36-37 0.738488646844992 2316 38-39 0.779759857306793 2316 40-41 0.7807072368108479 2316 42-43 0.8620503102992032 2316 44-45 0.6907719175623086 2316 46-47 0.7047596312174562 2316 48-49 0.7874840183731493 2316 50-51 0.5702074874470995 2316 52-53 0.3415438601563281 2316 54-55 0.31441203426498276 2316 56-57 0.3980504968916776 2316 58-59 0.4300113806969108 2316 60-61 0.5268807467423713 2316 62-63 0.5440172399681344 2316 64-65 0.5753597495788156 2316 66-67 0.5605487699990732 2316 68-69 0.5815807979856515 2316 70-71 0.439591934177443 2316 72-73 0.507888778313152 2316 74-75 0.5852568075656208 2316 76-77 0.6556904068399092 2316 78-79 0.7343672484934451 2316 80-81 0.8408994740659317 2316 82-83 0.8078168192014772 2316 84-85 0.847322071869538 2316 86-87 0.9207466669353295 2316 88-89 1.001855333433948 2316 90-91 1.005694018976726 2316 92-93 1.0449072572235742 2316 94-95 1.1017086554157594 2316 96-97 1.2015463086369138 2316 98-99 1.2964039164119434 2316 100 1.1767484639599672 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 51.0 1 673.0 2 352.0 3 245.0 4 187.0 5 153.0 6 238.0 7 1374.0 8 4487.0 9 8320.0 10 11986.0 11 16571.0 12 21852.0 13 27277.0 14 32678.0 15 39391.0 16 48053.0 17 61271.0 18 74903.0 19 88768.0 20 105565.0 21 124840.0 22 137234.0 23 140666.0 24 142535.0 25 148769.0 26 164353.0 27 187686.0 28 218083.0 29 256181.0 30 304806.0 31 371992.0 32 461917.0 33 591669.0 34 807850.0 35 1067837.0 36 1529072.0 37 2172645.0 38 2018032.0 39 554912.0 40 507.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.08697905336299 19.130525755362292 12.475134190009479 23.30736100126524 2 15.818009056237809 20.352021590639673 41.21289905242497 22.617070300697545 3 17.830715709535735 28.38453578355101 29.90904204907709 23.87570645783617 4 11.894047023377723 17.11637351574133 39.28368087442236 31.70589858645859 5 12.433090164904218 38.46194231128155 34.558754511418684 14.546213012395553 6 28.766323477372328 37.87051775727157 19.863620469074224 13.499538296281871 7 26.805576414531 32.91463122387736 23.635005631713966 16.64478672987767 8 26.50916795189357 34.586272520366386 20.89155800657413 18.01300152116592 9 28.116139278297485 15.105616411030127 20.682910251885893 36.09533405878649 10-11 24.865176447257433 25.89930801909957 29.155624685531745 20.079890848111248 12-13 25.467155394202184 24.313661712255342 28.62405483788924 21.595128055653234 14-15 23.08802433849436 25.40021814286524 26.694372836750617 24.817384681889777 16-17 21.887740576047506 27.625777137331482 27.56139620920708 22.92508607741393 18-19 21.5244498013945 27.371510923930437 29.499585474545825 21.60445380012924 20-21 22.89212951554959 26.633284963396843 28.84075674125115 21.633828779802418 22-23 22.657470971019876 26.577958480878177 28.632789189363894 22.13178135873805 24-25 22.093382607020352 26.529454569690152 29.136200293401547 22.24096252988795 26-27 22.010665993952692 26.721022982947563 28.800515453408565 22.46779556969118 28-29 21.759775782078265 27.380700555416592 28.868748161955164 21.990775500549976 30-31 22.91177723625626 26.667864486851617 28.75500615648039 21.66535212041174 32-33 22.73032017241986 26.84355778392925 28.43690916976892 21.989212873881975 34-35 22.673845844037942 26.95165673082418 28.83050957799307 21.543987847144813 36-37 22.423237449808003 26.871971896207615 28.71303137076489 21.99175928321949 38-39 22.409764915309804 27.02608801078672 28.779557650619203 21.784589423284277 40-41 22.291757086807394 26.40929719232197 29.163714296874353 22.13523142399628 42-43 22.113352375101254 26.707806554048624 28.478191905968874 22.70064916488125 44-45 21.7228872630526 27.19718822346656 28.79287726824889 22.28704724523195 46-47 21.79909353275088 26.577413222623473 28.518108868694735 23.105384375930907 48-49 22.11886169663528 26.61759946001647 28.576124236408607 22.687414606939644 50-51 22.022726066197055 26.93665341827976 28.23827686901313 22.802343646510064 52-53 21.608178887217502 27.28271590897861 28.056476251842206 23.05262895196168 54-55 21.581909406760943 26.979533770691553 28.36447914278626 23.074077679761242 56-57 22.079864577459254 26.787230782649647 28.07247782489813 23.060426814992972 58-59 21.71160401760139 26.890130183822304 28.462049368322432 22.936216430253875 60-61 22.11742385326816 27.181773599184062 27.912727465154962 22.78807508239281 62-63 22.083515780907508 26.738161414932694 27.98448889650474 23.193833907655062 64-65 21.64278949013761 26.44928808902386 28.161735328769282 23.74618709206925 66-67 21.73643666087432 26.32086722437556 27.940967621354606 24.001728493395515 68-69 21.36338973038953 26.591380803876092 28.124421016605762 23.920808449128618 70-71 21.547098438308453 26.193831207208994 28.315455738911094 23.94361461557146 72-73 21.638102234508935 26.484062661905348 27.974141455963856 23.903693647621854 74-75 21.729658997363437 26.53515533762597 27.81978215442743 23.915403510583165 76-77 21.530568639113497 26.17961754706062 28.017977444928494 24.27183636889739 78-79 21.27987002494175 26.240318222881605 28.21719666137244 24.262615090804207 80-81 21.37210747020158 26.344603085898182 28.03268182053952 24.250607623360718 82-83 21.85707186930008 25.95867108126924 28.283667658961487 23.9005893904692 84-85 21.935358885306794 26.236755935487476 27.59326895610366 24.234616223102062 86-87 21.395782203044977 26.51636481090014 27.95358245753321 24.134270528521675 88-89 21.685407722357336 25.71742125920386 27.991248103863985 24.605922914574823 90-91 22.058151930809967 25.495738208513284 28.09397312499908 24.352136735677664 92-93 22.14283890235729 25.6701520773268 27.87889659687891 24.308112423437 94-95 21.616426964290138 25.498223080957576 28.01335644952451 24.871993505227767 96-97 21.72790942343431 25.36092344958928 27.975717247988264 24.93544987898815 98-99 21.62241212835855 25.58725467956961 27.714006349314808 25.076326842757034 100 21.93860996163154 25.260365182395677 27.5015049369216 25.299519919051182 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1445.0 1 1105.5 2 2319.5 3 3479.5 4 2382.0 5 1876.0 6 2463.5 7 4314.5 8 7146.5 9 8538.0 10 7892.5 11 7445.5 12 8118.0 13 9152.5 14 10354.0 15 11562.5 16 12774.0 17 14021.0 18 15292.0 19 16443.5 20 17889.0 21 19814.0 22 22277.5 23 26105.0 24 30621.0 25 36648.0 26 46099.5 27 57520.0 28 71394.5 29 92190.0 30 119594.5 31 146073.5 32 180425.0 33 215168.0 34 245339.5 35 277364.0 36 311303.5 37 355684.0 38 375697.0 39 366662.0 40 357800.0 41 350749.5 42 352189.5 43 364915.5 44 385856.5 45 418268.5 46 445442.0 47 465241.0 48 486770.5 49 496880.5 50 498241.0 51 498750.5 52 488977.5 53 468371.0 54 441798.5 55 400206.5 56 357154.5 57 310239.0 58 262029.5 59 223077.5 60 177663.0 61 135238.0 62 105310.5 63 81987.5 64 59877.5 65 40619.0 66 28218.0 67 20589.0 68 15376.5 69 11691.5 70 9000.5 71 6644.0 72 4972.0 73 3816.5 74 2971.0 75 2205.5 76 1542.5 77 1048.5 78 722.0 79 518.5 80 363.0 81 287.5 82 233.0 83 170.5 84 127.5 85 116.5 86 97.5 87 72.5 88 55.0 89 43.0 90 40.0 91 33.0 92 21.0 93 15.5 94 19.5 95 17.5 96 10.0 97 9.0 98 5.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2218486702766395 2 0.14941426744274913 3 0.023187714763651474 4 0.022141337743631098 5 0.027038382197326447 6 0.05446183113802039 7 0.061460000647916645 8 0.05403490931385208 9 0.04134444881504499 10-11 0.04343720285508574 12-13 0.04218992144722145 14-15 0.020358311301516385 16-17 0.018638067480602892 18-19 0.01844134860083906 20-21 0.02186509421034572 22-23 0.025129790512809287 24-25 0.02194880437194735 26-27 0.019433314015818375 28-29 0.018780374755325662 30-31 0.017265220830336162 32-33 0.025456260143055645 34-35 0.026021303733866647 36-37 0.024832619439123505 38-39 0.023225384336372206 40-41 0.024204793227111276 42-43 0.030449571282592867 44-45 0.039979973180938426 46-47 0.044701226295270347 48-49 0.040817074796954726 50-51 0.02841959986375334 52-53 0.04301865204707759 54-55 0.0415913937917698 56-57 0.04420315083374065 58-59 0.04748458916852454 60-61 0.024175494670550707 62-63 0.02555252682889752 64-65 0.02645659657419512 66-67 0.031717780230857556 68-69 0.0355977462210931 70-71 0.03959909194565101 72-73 0.04327396803996256 74-75 0.024941442649205618 76-77 0.018725963150284602 78-79 0.018006055760510585 80-81 0.02110751724785097 82-83 0.01913614294213259 84-85 0.011593857381825737 86-87 0.013540118639063632 88-89 0.01911521540173218 90-91 0.01970118653294359 92-93 0.01997324455814889 94-95 0.023748572846382394 96-97 0.024577303446238527 98-99 0.03005194801498512 100 0.030303078499790016 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.1945981E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.79441595143195 #Duplication Level Percentage of deduplicated Percentage of total 1 74.43194499284587 42.27318843997791 2 14.753058251638693 16.75782653798547 3 4.964833345141771 8.459244304005633 4 2.2134744424525574 5.028519527300579 5 1.0968137483312466 3.114644812198701 6 0.6302501111355278 2.147681217916043 7 0.40313709153822774 1.6027154963590824 8 0.27291768792844256 1.2400160550968853 9 0.19853266023511085 1.0147991836803478 >10 0.9512279047354413 9.392395393847355 >50 0.049278570403535914 1.912436117093451 >100 0.03008496300999804 3.3082737536764553 >500 0.0030688916252898553 1.1986762606821977 >1k 0.00125212636940975 1.2505832214960992 >5k 6.260630446918762E-5 0.21050861132952872 >10k+ 6.260630446918762E-5 1.0884910673543866 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 41058 0.34369718150397194 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 29496 0.2469114926601675 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21284 0.178168707952909 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.371016160162987E-6 2 0.0 0.0 0.0 0.0 8.371016160162987E-6 3 0.0 0.0 0.0 0.0 8.371016160162987E-6 4 0.0 0.0 0.0 8.371016160162987E-6 1.6742032320325974E-5 5 0.0 0.0 0.0 8.371016160162987E-6 1.6742032320325974E-5 6 1.6742032320325974E-5 0.0 0.0 8.371016160162987E-6 2.511304848048896E-5 7 1.6742032320325974E-5 0.0 0.0 8.371016160162987E-6 2.511304848048896E-5 8 1.6742032320325974E-5 0.0 0.0 8.371016160162987E-6 3.348406464065195E-5 9 1.6742032320325974E-5 0.0 0.0 4.185508080081494E-5 5.022609696097792E-5 10-11 1.6742032320325974E-5 0.0 0.0 6.27826212012224E-5 5.022609696097792E-5 12-13 1.6742032320325974E-5 0.0 0.0 1.0463770200203735E-4 5.022609696097792E-5 14-15 1.6742032320325974E-5 0.0 0.0 1.255652424024448E-4 7.533914544146689E-5 16-17 1.6742032320325974E-5 0.0 0.0 1.7579133936342274E-4 1.0882321008211883E-4 18-19 5.022609696097792E-5 0.0 0.0 2.636870090451341E-4 1.297507504825263E-4 20-21 5.022609696097792E-5 0.0 0.0 5.190030019301053E-4 1.3812176664268927E-4 22-23 5.859711312114091E-5 0.0 0.0 0.001661646707792353 1.464927828028523E-4 24-25 6.69681292813039E-5 0.0 0.0 0.003892522514475789 1.5904930704309676E-4 26-27 6.69681292813039E-5 0.0 0.0 0.0068349346947730785 1.5904930704309676E-4 28-29 6.69681292813039E-5 0.0 0.0 0.01645741777088043 1.5904930704309676E-4 30-31 6.69681292813039E-5 0.0 0.0 0.05505617328539196 1.5904930704309676E-4 32-33 7.533914544146689E-5 0.0 0.0 0.13910954654958851 1.8416235552358573E-4 34-35 8.371016160162987E-5 0.0 0.0 0.24876985824772363 2.0508989592399318E-4 36-37 9.626668584187436E-5 0.0 0.0 0.41881868052527454 2.1764642016423765E-4 38-39 1.0045219392195584E-4 0.0 0.0 0.727566869560566 2.2183192824431916E-4 40-41 1.0882321008211883E-4 0.0 0.0 1.117241857324233 2.2601743632440064E-4 42-43 1.0882321008211883E-4 0.0 0.0 1.481435471896364 2.4694497672480816E-4 44-45 1.0882321008211883E-4 0.0 0.0 1.8622915941352995 2.595015009650526E-4 46-47 1.0882321008211883E-4 0.0 0.0 2.295056387583406 2.678725171252156E-4 48-49 1.0882321008211883E-4 4.1855080800814935E-6 0.0 2.776979973432069 3.0135658176586754E-4 50-51 1.0882321008211883E-4 8.371016160162987E-6 0.0 3.3014032083258797 3.0135658176586754E-4 52-53 1.0882321008211883E-4 8.371016160162987E-6 0.0 3.8231644600807586 3.180986140861935E-4 54-55 1.0882321008211883E-4 8.371016160162987E-6 0.0 4.369800186355562 3.39026154486601E-4 56-57 1.0882321008211883E-4 8.371016160162987E-6 0.0 4.955402992855923 3.4739717064676397E-4 58-59 1.1719422624228182E-4 8.371016160162987E-6 0.0 5.616311460733112 3.515826787268455E-4 60-61 1.4230727472277078E-4 8.371016160162987E-6 0.0 6.289378829582937 3.5995369488700844E-4 62-63 1.4230727472277078E-4 1.6742032320325974E-5 0.0 7.011558950244438 3.6832471104717145E-4 64-65 1.4230727472277078E-4 1.6742032320325974E-5 0.0 7.659291438685529 3.766957272073344E-4 66-67 1.4230727472277078E-4 1.6742032320325974E-5 0.0 8.332722946738322 3.8506674336749743E-4 68-69 1.5067829088293377E-4 1.6742032320325974E-5 0.0 9.089759978690742 3.8506674336749743E-4 70-71 1.5067829088293377E-4 1.6742032320325974E-5 0.0 9.875271022111956 3.976232676077419E-4 72-73 1.5067829088293377E-4 1.6742032320325974E-5 0.0 10.623723576992127 5.022609696097792E-4 74-75 1.5067829088293377E-4 1.6742032320325974E-5 0.0 11.33723969592786 5.022609696097792E-4 76-77 1.5067829088293377E-4 2.0927540400407465E-5 0.0 12.077618405721555 5.022609696097792E-4 78-79 1.5904930704309676E-4 2.511304848048896E-5 0.0 12.858383082979959 5.106319857699422E-4 80-81 1.5904930704309676E-4 2.511304848048896E-5 8.371016160162987E-6 13.649268318775997 5.190030019301053E-4 82-83 1.6742032320325975E-4 2.511304848048896E-5 1.6742032320325974E-5 14.465798162578695 5.524870665707571E-4 84-85 1.6742032320325975E-4 2.9298556560570454E-5 1.6742032320325974E-5 15.245658770091799 5.524870665707571E-4 86-87 1.6742032320325975E-4 3.348406464065195E-5 1.6742032320325974E-5 16.013486041874668 5.608580827309201E-4 88 1.7579133936342274E-4 3.348406464065195E-5 1.6742032320325974E-5 16.593145426901316 5.692290988910831E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 12955 0.0 53.555912 2 GGTATCA 21055 0.0 47.731167 1 ACCTGGG 14780 0.0 45.766495 3 GTATCAA 38995 0.0 42.216328 1 CCTGGGG 13765 0.0 34.558918 4 GTACCTG 20705 0.0 33.860565 1 ATCAACG 48515 0.0 32.972145 3 CAACGCA 48470 0.0 32.924755 5 TATCAAC 50160 0.0 32.328014 2 GTACATG 51870 0.0 31.94645 1 TCAACGC 51190 0.0 31.31303 4 AACGCAG 52450 0.0 30.910841 6 TACATGG 52825 0.0 30.768366 2 ACATGGG 52830 0.0 29.674028 3 TATCACG 1030 0.0 29.247593 2 CATGGGG 37385 0.0 28.290522 4 GTACTAG 5245 0.0 28.026932 1 ACGCAGA 57250 0.0 27.892927 7 CGCAGAG 57940 0.0 27.478241 8 TGGGGGG 30280 0.0 27.384562 6 >>END_MODULE