FastQCFastQC Report
Mon 6 Jun 2016
ERR1211418_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211418_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11945981
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT372920.31217193464479814No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT286810.24008911448963463No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT211410.1769716526420057No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT126220.10565896597357723No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG128000.054.5552332
GGTATCA208950.049.21091
ACCTGGG146100.046.8647163
GTATCAA379800.041.4957771
CCTGGGG124350.037.9049074
GTACCTG217550.032.5261231
ATCAACG483750.031.8729233
CAACGCA486350.031.7174765
TAACGCA15350.031.533284
TATCAAC502050.031.2814622
CTGGGGG96150.030.5915645
TCAACGC510900.030.2805824
AACGCAG523400.029.965286
GTACATG506600.029.5039541
TACATGG515900.028.5470352
ACATGGG513500.027.6286283
TGGGGGG273600.027.6144076
ACGCAGA576100.026.8897557
CGCAGAG582950.026.4528858
CATGGGG371000.026.2962464
Produced by FastQC (version 0.11.5)