FastQCFastQC Report
Mon 6 Jun 2016
ERR1211417_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211417_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24163976
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT353100.14612661426248727No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260490.10780096785396576No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA310050.043.4380341
GTATCAA541300.041.311311
CAACGCA619250.034.6533555
GTACTAG148050.033.4093971
ATCAACG644750.033.3630073
TATCAAC697800.031.8064612
AACGCAG696250.031.536956
TCAACGC723500.029.9393044
ACGCAGA750200.028.5172967
GTACATG899550.028.2837261
CGCAGAG770300.027.6188938
TACATGG913200.027.4735832
ACATGGG908650.026.7411693
TAGGCAT194900.026.506745
GCATCGT167000.026.317638
CTAGGCA210000.026.1003934
TAGTACT202300.025.560224
CTGTGCG205050.024.9872959
CTAGTAC219050.024.528643
GCAGAGT857200.024.0897089
Produced by FastQC (version 0.11.5)