FastQCFastQC Report
Mon 6 Jun 2016
ERR1211417_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211417_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24163976
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT322870.13361625586782572No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT257470.10655117353203794No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA313800.044.0795631
GTATCAA530150.039.1276971
CAACGCA620600.031.9358225
ATCAACG641650.030.93543
TATCAAC702700.029.2838862
AACGCAG699750.029.2027916
TCAACGC724050.027.6424354
GTACATG895250.027.1514951
TAGGCAT191600.026.9026095
TACATGG899850.026.5078952
ACGCAGA763600.025.8317857
ACATGGG899950.025.5706483
CTAGGCA209800.025.0646574
CGCAGAG785950.024.977648
CTAACGC66300.024.8784683
ATTTAGG217800.024.4363371
AGGGCTA217100.024.3270665
TCTAACG69550.024.0534042
GCTATTG217300.023.8286678
TAACGCC69950.023.7150574
Produced by FastQC (version 0.11.5)