##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211417_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 24163976 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.07578210638845 33.0 31.0 34.0 30.0 34.0 2 32.222542225666835 33.0 31.0 34.0 30.0 34.0 3 32.303563039460066 34.0 31.0 34.0 30.0 34.0 4 35.9450524615651 37.0 35.0 37.0 35.0 37.0 5 35.79830558514046 37.0 35.0 37.0 35.0 37.0 6 35.825241591036175 37.0 35.0 37.0 35.0 37.0 7 35.749437675322966 37.0 35.0 37.0 33.0 37.0 8 35.733857457895176 37.0 35.0 37.0 33.0 37.0 9 37.32546498142524 39.0 37.0 39.0 34.0 39.0 10-11 37.298502469130085 39.0 37.0 39.0 34.0 39.0 12-13 37.21031139494593 39.0 37.0 39.0 33.0 39.0 14-15 38.43106747002231 40.0 38.0 41.0 33.5 41.0 16-17 38.42292437718031 40.0 38.0 41.0 34.0 41.0 18-19 38.40531657538478 40.0 38.0 41.0 33.5 41.0 20-21 38.355964804798674 40.0 38.0 41.0 34.0 41.0 22-23 38.25078085245573 40.0 38.0 41.0 34.0 41.0 24-25 38.12534257607275 40.0 37.5 41.0 33.0 41.0 26-27 38.00266150322281 40.0 37.0 41.0 33.0 41.0 28-29 37.95318582918639 40.0 37.0 41.0 33.0 41.0 30-31 38.0304149656497 40.0 37.0 41.0 33.0 41.0 32-33 37.91836347627559 40.0 37.0 41.0 33.0 41.0 34-35 37.8292319318642 40.0 37.0 41.0 33.0 41.0 36-37 37.701041024871074 40.0 37.0 41.0 33.0 41.0 38-39 37.59415642111216 40.0 37.0 41.0 32.0 41.0 40-41 37.53760786304373 40.0 37.0 41.0 32.0 41.0 42-43 37.42602577075891 40.0 36.0 41.0 32.0 41.0 44-45 37.28045305954616 39.5 36.0 41.0 31.0 41.0 46-47 37.08455793864387 39.0 36.0 41.0 31.0 41.0 48-49 36.88239629935073 39.0 35.5 41.0 31.0 41.0 50-51 36.702217776577825 39.0 35.0 40.5 30.5 41.0 52-53 36.48152566034663 39.0 35.0 40.0 30.0 41.0 54-55 36.26380010888937 38.0 35.0 40.0 30.0 41.0 56-57 36.011343207756866 38.0 35.0 40.0 29.0 41.0 58-59 35.71750640291978 38.0 34.0 40.0 29.0 41.0 60-61 35.44907185390351 37.0 34.0 40.0 28.0 41.0 62-63 35.14280760335137 37.0 34.0 40.0 28.0 41.0 64-65 34.78100648668084 36.0 34.0 39.0 28.0 41.0 66-67 34.42827660067201 36.0 33.0 39.0 27.0 40.0 68-69 34.07168242924922 35.0 33.0 38.5 26.0 40.0 70-71 33.73334084175551 35.0 33.0 38.0 26.0 40.0 72-73 33.405127554339565 35.0 32.0 37.0 26.0 39.5 74-75 33.06138251006374 35.0 32.0 37.0 26.0 39.0 76-77 32.08304059729243 34.0 30.5 35.5 24.5 38.0 78-79 32.59808234787189 34.5 32.0 36.0 26.0 38.0 80-81 32.513664183410874 35.0 32.0 35.5 25.5 37.0 82-83 32.27105460624526 34.5 32.0 35.0 25.0 37.0 84-85 32.029798552191906 34.0 31.5 35.0 25.0 36.5 86-87 31.80111228383938 34.0 31.0 35.0 25.0 36.0 88-89 31.595601112995645 34.0 31.0 35.0 24.0 36.0 90-91 31.39551545656228 34.0 31.0 35.0 24.0 35.0 92-93 31.225322831805496 34.0 31.0 35.0 24.0 35.0 94-95 31.076499268994475 34.0 31.0 35.0 23.0 35.0 96-97 30.92535789639917 34.0 30.0 35.0 23.0 35.0 98-99 30.750680558530597 34.0 30.0 35.0 21.5 35.0 100 28.835756499675384 32.0 26.0 34.0 18.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.26174461584489706 1101 2 -0.24379456095655883 1101 3 -0.3001301303714712 1101 4 -0.16737223528692624 1101 5 -0.17432180270814257 1101 6 -0.2545362933597204 1101 7 -0.28379284449066944 1101 8 -0.26131381419575916 1101 9 -0.40958804375456026 1101 10-11 -0.3832101978303726 1101 12-13 -0.41579907333184707 1101 14-15 -0.6373902864349077 1101 16-17 -0.6318330954850637 1101 18-19 -0.6288962396381237 1101 20-21 -0.6329087741580821 1101 22-23 -0.7055926794262817 1101 24-25 -0.7382133983154588 1101 26-27 -0.7009004331218236 1101 28-29 -0.7492748820503152 1101 30-31 -0.6735868961034583 1101 32-33 -0.6226555316337041 1101 34-35 -0.6794301800606846 1101 36-37 -0.7043087748467372 1101 38-39 -0.7192924782106971 1101 40-41 -0.7707635818780147 1101 42-43 -0.7085848758686311 1101 44-45 -0.7728980885239167 1101 46-47 -0.8388439101324181 1101 48-49 -0.8368420613229901 1101 50-51 -0.8618253547907244 1101 52-53 -0.8545221414265072 1101 54-55 -0.818490801421504 1101 56-57 -0.8359899740102676 1101 58-59 -0.8443837836178432 1101 60-61 -0.8125372567729343 1101 62-63 -0.9175533745240614 1101 64-65 -0.8769016018559057 1101 66-67 -0.8849968573352598 1101 68-69 -0.9183666376209416 1101 70-71 -0.9691502644288121 1101 72-73 -1.0170719534830823 1101 74-75 -0.9892679519943357 1101 76-77 -0.8743674048255805 1101 78-79 -0.8911617305543267 1101 80-81 -0.8000549295985273 1101 82-83 -0.8388827341675196 1101 84-85 -0.8915056906184269 1101 86-87 -0.9117187944993113 1101 88-89 -0.9357143368671181 1101 90-91 -1.0062502400061248 1101 92-93 -1.1345749273605605 1101 94-95 -1.2148810443570532 1101 96-97 -1.1730264130366486 1101 98-99 -1.29171883700079 1101 100 -1.1923945206681559 1102 1 -0.287946073965216 1102 2 -0.23926184477044643 1102 3 -0.3035986601343339 1102 4 -0.1487449790650004 1102 5 -0.167632062525918 1102 6 -0.2639976077150763 1102 7 -0.26098352115428725 1102 8 -0.3095946706196173 1102 9 -0.404346848442664 1102 10-11 -0.4096022582692953 1102 12-13 -0.45907004820224984 1102 14-15 -0.5842340402532145 1102 16-17 -0.6193716072941413 1102 18-19 -0.6642359528134278 1102 20-21 -0.6514833283976813 1102 22-23 -0.6604069052873243 1102 24-25 -0.7186576786647478 1102 26-27 -0.8209539476953935 1102 28-29 -0.7984076856236513 1102 30-31 -0.7397409449408983 1102 32-33 -0.7464868009622379 1102 34-35 -0.7828892750800378 1102 36-37 -0.7238596455147785 1102 38-39 -0.7930438912539799 1102 40-41 -0.7804757636129551 1102 42-43 -0.7948806646845767 1102 44-45 -0.8238792035953608 1102 46-47 -0.8715826611506898 1102 48-49 -0.915270528906305 1102 50-51 -0.9268365448113656 1102 52-53 -0.9093554108406039 1102 54-55 -0.9928233002931393 1102 56-57 -0.9606717864363858 1102 58-59 -1.0264603595041564 1102 60-61 -1.0609033164027721 1102 62-63 -1.0125287937906435 1102 64-65 -0.9928299088678472 1102 66-67 -1.017633854923801 1102 68-69 -1.082487885574288 1102 70-71 -1.0929439852249132 1102 72-73 -1.178710079565434 1102 74-75 -1.2080782007555726 1102 76-77 -0.9591599999177696 1102 78-79 -0.914125041689811 1102 80-81 -0.8374961688138782 1102 82-83 -0.849089114875575 1102 84-85 -0.8874081880634463 1102 86-87 -0.9530707518804711 1102 88-89 -0.9685987939010872 1102 90-91 -0.9753425394419857 1102 92-93 -1.0326168547881664 1102 94-95 -1.0827372995986693 1102 96-97 -1.145443843959388 1102 98-99 -1.1761323969130473 1102 100 -1.072580489433573 1103 1 -0.35103434749111884 1103 2 -0.3273502087452975 1103 3 -0.36186535843228995 1103 4 -0.21176812934515965 1103 5 -0.18928476645858439 1103 6 -0.31973780834636045 1103 7 -0.3407991655152074 1103 8 -0.3889868846704232 1103 9 -0.4842893797625578 1103 10-11 -0.49445364805621494 1103 12-13 -0.5794617386828378 1103 14-15 -0.7284080552512009 1103 16-17 -0.8009678426536837 1103 18-19 -0.7135298566057173 1103 20-21 -0.6862488692074749 1103 22-23 -0.824436791171749 1103 24-25 -0.7813231033380887 1103 26-27 -0.8781368737241664 1103 28-29 -0.8686841312175702 1103 30-31 -0.752071139954019 1103 32-33 -0.7617798452310609 1103 34-35 -0.7783921531657114 1103 36-37 -0.728138010177048 1103 38-39 -0.8397410056222867 1103 40-41 -0.9128159545274386 1103 42-43 -0.9076132024806114 1103 44-45 -0.9009776322127863 1103 46-47 -0.9707847463349921 1103 48-49 -0.9632830940747752 1103 50-51 -1.012577435438942 1103 52-53 -1.0603618750980246 1103 54-55 -1.0543705174889766 1103 56-57 -1.1112899016559439 1103 58-59 -1.157593186116614 1103 60-61 -1.0467899848990925 1103 62-63 -1.1350677424016808 1103 64-65 -1.0999101624143535 1103 66-67 -1.1434444067502696 1103 68-69 -1.1340479878694723 1103 70-71 -1.1322430859555865 1103 72-73 -1.1647662752411492 1103 74-75 -1.1914134981233033 1103 76-77 -1.1182082805348692 1103 78-79 -0.9851087334852622 1103 80-81 -0.9189659953017753 1103 82-83 -0.9377628773917728 1103 84-85 -0.9238819865524839 1103 86-87 -0.9192619025617077 1103 88-89 -0.9760878379486932 1103 90-91 -1.1547508146424832 1103 92-93 -1.069453095150834 1103 94-95 -1.0715932133837605 1103 96-97 -1.2204480562963127 1103 98-99 -1.2410052282702004 1103 100 -1.2546692193390463 1104 1 -0.41070450017881655 1104 2 -0.36854090130357875 1104 3 -0.3814054497363699 1104 4 -0.2765112120054596 1104 5 -0.2840383479478916 1104 6 -0.341265971655595 1104 7 -0.32172859564266787 1104 8 -0.3393954337706262 1104 9 -0.498801823253892 1104 10-11 -0.4738331592279792 1104 12-13 -0.5502461007563966 1104 14-15 -0.7160580668928347 1104 16-17 -0.7389140785624946 1104 18-19 -0.7481892028641397 1104 20-21 -0.7081696394425379 1104 22-23 -0.7736229797327212 1104 24-25 -0.8450184663640741 1104 26-27 -0.8665105502902506 1104 28-29 -0.868640525640096 1104 30-31 -0.8501141080259984 1104 32-33 -0.7886977630901129 1104 34-35 -0.8718680881414613 1104 36-37 -0.7996863675395787 1104 38-39 -0.9317747951625535 1104 40-41 -0.9753429891605947 1104 42-43 -0.8784144850442672 1104 44-45 -0.9539777256394615 1104 46-47 -0.9164964767150039 1104 48-49 -0.9322871834344895 1104 50-51 -0.9353581938626121 1104 52-53 -0.8947110936225613 1104 54-55 -0.8650301940485647 1104 56-57 -0.9176738889438454 1104 58-59 -1.040218555008039 1104 60-61 -0.9704356082420276 1104 62-63 -0.9664899161733516 1104 64-65 -0.9645914598173917 1104 66-67 -1.0478892147223178 1104 68-69 -1.0019209330826868 1104 70-71 -0.9915908262830087 1104 72-73 -0.9930284211752038 1104 74-75 -0.9693071230006609 1104 76-77 -0.9250593414027861 1104 78-79 -0.8386397042471714 1104 80-81 -0.8971825955246366 1104 82-83 -0.9113046359405175 1104 84-85 -0.8856896048405467 1104 86-87 -0.7986155353853661 1104 88-89 -0.9001077804775122 1104 90-91 -0.8775136491012816 1104 92-93 -1.0528428325925034 1104 94-95 -1.0821257060122846 1104 96-97 -1.0997650352758406 1104 98-99 -1.0721717366328427 1104 100 -1.1422621121079395 1105 1 -0.40550884718416924 1105 2 -0.391898901561202 1105 3 -0.4249116498314507 1105 4 -0.2484352662675633 1105 5 -0.2849740551105384 1105 6 -0.3263568516658779 1105 7 -0.3673093903182547 1105 8 -0.3357541265086468 1105 9 -0.5370848323112156 1105 10-11 -0.5247401523046804 1105 12-13 -0.5442524944992613 1105 14-15 -0.7169585139981578 1105 16-17 -0.6721430619661035 1105 18-19 -0.6992087254706405 1105 20-21 -0.7771183924281218 1105 22-23 -0.7736702802287567 1105 24-25 -0.8156531807949321 1105 26-27 -0.7977526237523378 1105 28-29 -0.8109184501649978 1105 30-31 -0.7888116431764445 1105 32-33 -0.7178735937561527 1105 34-35 -0.7355937164021782 1105 36-37 -0.9054325157404648 1105 38-39 -0.8342356337948544 1105 40-41 -0.8100070590200517 1105 42-43 -0.7971168595797096 1105 44-45 -0.8583137127403475 1105 46-47 -0.9589779536964116 1105 48-49 -0.8454999104363949 1105 50-51 -0.8360186437178285 1105 52-53 -0.8907890928117368 1105 54-55 -0.8900653735397697 1105 56-57 -0.9074747477903315 1105 58-59 -0.9041354939217854 1105 60-61 -0.9639711906062516 1105 62-63 -0.9416905618813232 1105 64-65 -0.9117406369248258 1105 66-67 -0.9945466059684662 1105 68-69 -0.983712902711126 1105 70-71 -1.0437694215049405 1105 72-73 -1.0102510946327996 1105 74-75 -0.98969789153211 1105 76-77 -0.9936044601866918 1105 78-79 -0.9357158267938175 1105 80-81 -0.8143591823354797 1105 82-83 -0.8515667245601897 1105 84-85 -0.76652316707127 1105 86-87 -0.8012123358525258 1105 88-89 -0.8218818123491118 1105 90-91 -0.87605928015223 1105 92-93 -0.9611547644192058 1105 94-95 -0.9444360161331069 1105 96-97 -0.9625442058016738 1105 98-99 -1.0189430879879993 1105 100 -1.0280732923852334 1106 1 -0.3584232721325513 1106 2 -0.3570496312413276 1106 3 -0.38220479676428454 1106 4 -0.20336359215798439 1106 5 -0.2538283321453747 1106 6 -0.30118904170906546 1106 7 -0.3791054581352711 1106 8 -0.3700747978415393 1106 9 -0.4978266045306867 1106 10-11 -0.49317612777255704 1106 12-13 -0.5711902875727048 1106 14-15 -0.7129490103491278 1106 16-17 -0.7562001369147353 1106 18-19 -0.7281130065590773 1106 20-21 -0.7186996788948932 1106 22-23 -0.722174529086054 1106 24-25 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40-41 1.0868479526062913 2311 42-43 1.201746426236781 2311 44-45 1.1742031742093957 2311 46-47 1.2144697692643476 2311 48-49 1.222054800470012 2311 50-51 1.2218524587409547 2311 52-53 1.2429861681422665 2311 54-55 1.2342816673313024 2311 56-57 1.2240772074482322 2311 58-59 1.2679696901833353 2311 60-61 1.2556639696712395 2311 62-63 1.259259410490131 2311 64-65 1.2677216109846583 2311 66-67 1.3322388855321634 2311 68-69 1.3384027738528417 2311 70-71 1.3535293780108404 2311 72-73 1.337606695019744 2311 74-75 1.2565704008002498 2311 76-77 1.160187632619671 2311 78-79 1.1109232406070575 2311 80-81 1.1335239928390735 2311 82-83 1.1325531689501318 2311 84-85 1.1677554883608572 2311 86-87 1.232882938708137 2311 88-89 1.2532180368916634 2311 90-91 1.317941668481037 2311 92-93 1.3648342839770606 2311 94-95 1.3848902522247037 2311 96-97 1.4161509219923225 2311 98-99 1.4561436496717768 2311 100 1.5341443183365584 2312 1 0.1439255572820315 2312 2 0.14479968785544628 2312 3 0.32639056415777645 2312 4 0.25400886976107984 2312 5 0.31289478930946757 2312 6 0.4146809684814414 2312 7 0.4631919109187308 2312 8 0.27995265325920116 2312 9 0.4166298113224727 2312 10-11 0.26716473109134853 2312 12-13 0.42675953561044366 2312 14-15 0.7784425179042458 2312 16-17 0.8320651048070147 2312 18-19 0.866681274881401 2312 20-21 0.8617948034865606 2312 22-23 0.501005252216487 2312 24-25 0.4587469765213683 2312 26-27 0.7778928705480084 2312 28-29 0.9334086534304262 2312 30-31 0.8916918475301614 2312 32-33 0.7519718534075892 2312 34-35 0.9014459808064927 2312 36-37 0.9857532946063756 2312 38-39 0.9710475982071358 2312 40-41 1.00882129851869 2312 42-43 0.8907788139573327 2312 44-45 0.842732139636297 2312 46-47 1.0302002784391746 2312 48-49 1.0773236161573934 2312 50-51 1.096045621207324 2312 52-53 1.1236752180621536 2312 54-55 1.12292209725301 2312 56-57 1.109331413204039 2312 58-59 1.1612631043148696 2312 60-61 1.1546223945344494 2312 62-63 1.1335132534853258 2312 64-65 1.1889354961507763 2312 66-67 1.1890270950566446 2312 68-69 1.1949983423793284 2312 70-71 1.0563755763300477 2312 72-73 1.1413041451149724 2312 74-75 1.209108513336652 2312 76-77 1.1130219820185907 2312 78-79 1.0177839632060355 2312 80-81 0.9182770397762638 2312 82-83 0.8903374800576671 2312 84-85 0.7215520300430605 2312 86-87 0.9385576318731239 2312 88-89 0.6175961090205639 2312 90-91 1.018501957278783 2312 92-93 1.1660838846884758 2312 94-95 1.2186809582475355 2312 96-97 1.2690181818397583 2312 98-99 1.2915492160317825 2312 100 1.3626900211348563 2313 1 -0.3372823699373271 2313 2 -0.01986839506628968 2313 3 0.24851218559599175 2313 4 0.08747571743676019 2313 5 0.22774626823825628 2313 6 0.3243777858715191 2313 7 0.16015457147045709 2313 8 0.10532580831826976 2313 9 0.2950788195589382 2313 10-11 0.38150003998770643 2313 12-13 0.5829092238382714 2313 14-15 0.6253264247496233 2313 16-17 0.7597737768595181 2313 18-19 0.6468215825347201 2313 20-21 0.8387626058918158 2313 22-23 0.8893921459911098 2313 24-25 0.748324874546519 2313 26-27 0.9185815899972951 2313 28-29 0.8216378557048003 2313 30-31 0.8195145604808758 2313 32-33 0.9948325796939912 2313 34-35 0.9972567603521796 2313 36-37 0.8455252359551935 2313 38-39 0.6175988129387875 2313 40-41 0.7824854651534565 2313 42-43 0.8166996265660984 2313 44-45 1.0114373005067563 2313 46-47 1.0685728265684062 2313 48-49 1.0800977204104854 2313 50-51 1.0027097487027277 2313 52-53 0.9339602573297014 2313 54-55 1.0352117536903833 2313 56-57 1.0925909876912172 2313 58-59 1.0050359558765507 2313 60-61 1.022713720087836 2313 62-63 1.0954537091347945 2313 64-65 1.1629359597437912 2313 66-67 1.153569306646986 2313 68-69 1.169397586251172 2313 70-71 1.059386350894144 2313 72-73 1.1523433344576404 2313 74-75 0.8217959492903901 2313 76-77 0.9658489309973142 2313 78-79 0.8385692883134581 2313 80-81 0.9334246434371352 2313 82-83 0.9485312132743715 2313 84-85 0.9977116536520718 2313 86-87 1.0731146847536124 2313 88-89 1.055715040407339 2313 90-91 1.146411026214789 2313 92-93 0.9007170965929561 2313 94-95 0.9413928339728521 2313 96-97 1.2062116032722052 2313 98-99 1.264065933922442 2313 100 1.317814472660448 2314 1 -0.3009517830873705 2314 2 -0.13387795922982093 2314 3 0.14525346214389856 2314 4 -0.11908533023792955 2314 5 0.19452091594120446 2314 6 0.21621980365867444 2314 7 0.13288486484155015 2314 8 0.15851225767379162 2314 9 0.22064437072270948 2314 10-11 0.41557182996429987 2314 12-13 0.5211864344455748 2314 14-15 0.6225482540792413 2314 16-17 0.8161939976406316 2314 18-19 0.8770034369072164 2314 20-21 0.5262919735267673 2314 22-23 0.7182536963060997 2314 24-25 0.918660159883963 2314 26-27 0.8889895501047178 2314 28-29 0.8295340449417807 2314 30-31 0.7845828200842178 2314 32-33 0.874437311733459 2314 34-35 0.9019263211654973 2314 36-37 0.8416052259997002 2314 38-39 0.9049474497572234 2314 40-41 0.916687857831171 2314 42-43 0.7384991928011928 2314 44-45 0.7745104914287992 2314 46-47 0.9112975752975814 2314 48-49 0.777287086963689 2314 50-51 0.9146550971566043 2314 52-53 0.9549579666714294 2314 54-55 1.0245621719826303 2314 56-57 0.8742306574727152 2314 58-59 0.9099419333612246 2314 60-61 0.9532132070237296 2314 62-63 0.649877243651936 2314 64-65 0.8301563350526493 2314 66-67 0.9523120289988185 2314 68-69 1.0269976101821428 2314 70-71 1.071842399473887 2314 72-73 1.058965633957989 2314 74-75 1.0620874398394093 2314 76-77 0.8676113478846084 2314 78-79 0.8458541576825667 2314 80-81 0.8672718582753802 2314 82-83 0.8404970657716504 2314 84-85 0.7320758252946753 2314 86-87 0.8870268237889221 2314 88-89 0.9413738488002572 2314 90-91 1.0814190463525186 2314 92-93 1.1113065121667915 2314 94-95 1.1482419131625683 2314 96-97 1.2229668773875524 2314 98-99 1.2271255040068603 2314 100 1.27337899294362 2315 1 -0.08913897700419326 2315 2 0.11370895489032051 2315 3 0.11910160628886501 2315 4 -0.06744628261300534 2315 5 0.24322041326956167 2315 6 0.34759711878501065 2315 7 0.400838874568322 2315 8 0.3811598785221051 2315 9 0.5785353407184886 2315 10-11 0.5566047079393144 2315 12-13 0.6031092621055336 2315 14-15 0.7143521962279635 2315 16-17 0.725416453271734 2315 18-19 0.7909590632469161 2315 20-21 0.8012622715355207 2315 22-23 0.9027148308679358 2315 24-25 0.831759482321992 2315 26-27 0.9329259869689253 2315 28-29 0.9541077209861584 2315 30-31 0.9229130143103177 2315 32-33 0.9297194987926787 2315 34-35 0.9490690915469457 2315 36-37 0.9812014726048801 2315 38-39 0.9732331334243582 2315 40-41 0.9645248973263065 2315 42-43 1.0300169772530055 2315 44-45 0.9052704807269834 2315 46-47 0.9544364409807571 2315 48-49 1.0667862341237608 2315 50-51 0.9442548672225612 2315 52-53 0.9698842462682506 2315 54-55 0.9025467572981256 2315 56-57 1.0446654082829028 2315 58-59 1.0567210090206487 2315 60-61 1.0895868900743082 2315 62-63 1.0840831132770958 2315 64-65 1.1148790443647485 2315 66-67 1.1332062908861928 2315 68-69 1.046957946944893 2315 70-71 0.6961761415754992 2315 72-73 0.9688169283177359 2315 74-75 1.1205310800228503 2315 76-77 1.047613838815213 2315 78-79 0.972376105764333 2315 80-81 0.9590430179379084 2315 82-83 0.9886533456095847 2315 84-85 0.8982080346514927 2315 86-87 0.8730008234616662 2315 88-89 0.9758265346266093 2315 90-91 1.0446310386155737 2315 92-93 1.0963225586030987 2315 94-95 1.15232411060882 2315 96-97 1.224896058359736 2315 98-99 1.1200629825196877 2315 100 1.0443723043210866 2316 1 0.5198004776704721 2316 2 0.5018161134058019 2316 3 0.5061721703863569 2316 4 0.3126525637041482 2316 5 0.410555486797449 2316 6 0.4087414923033066 2316 7 0.46589095174474693 2316 8 0.4835676638424502 2316 9 0.5185792834745655 2316 10-11 0.5073463389188433 2316 12-13 0.6467241638853451 2316 14-15 0.8127030013441683 2316 16-17 0.8312234728217618 2316 18-19 0.8483949759165057 2316 20-21 0.8740110062175361 2316 22-23 0.9048559224568109 2316 24-25 0.9651619208390443 2316 26-27 1.0318117002829226 2316 28-29 0.9715597592366478 2316 30-31 0.8816225254072023 2316 32-33 0.8223694567937301 2316 34-35 0.7328267538560596 2316 36-37 0.7419157634860625 2316 38-39 0.6800022829800128 2316 40-41 0.8072513630332452 2316 42-43 0.9502147855799095 2316 44-45 0.8531379337627243 2316 46-47 0.9157465528791349 2316 48-49 0.6615543469781286 2316 50-51 0.7344557385190598 2316 52-53 0.8941469424136699 2316 54-55 0.975755898074695 2316 56-57 1.0171880232297497 2316 58-59 0.9460437275328317 2316 60-61 0.9821557282360018 2316 62-63 0.9885166249701953 2316 64-65 1.0028127208640711 2316 66-67 0.9987043463662957 2316 68-69 0.9870085844276062 2316 70-71 0.8597599768360809 2316 72-73 0.9184901224106596 2316 74-75 0.9749362739760912 2316 76-77 0.8367961659453194 2316 78-79 0.8134410623583435 2316 80-81 0.7899782843359802 2316 82-83 0.7468967250463621 2316 84-85 0.7911501750675463 2316 86-87 0.8073206801505748 2316 88-89 0.7057232964377853 2316 90-91 0.813710481196086 2316 92-93 0.8377805421891047 2316 94-95 0.7561609166982954 2316 96-97 0.9156375307847853 2316 98-99 0.9938015248343426 2316 100 0.6794016521881687 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 5.0 11 20.0 12 102.0 13 448.0 14 1710.0 15 5036.0 16 11868.0 17 23100.0 18 38435.0 19 56303.0 20 79790.0 21 111534.0 22 137205.0 23 151753.0 24 170794.0 25 204901.0 26 255474.0 27 319240.0 28 394868.0 29 485559.0 30 596618.0 31 744603.0 32 942138.0 33 1235518.0 34 1729298.0 35 2402904.0 36 3702987.0 37 5392085.0 38 4317393.0 39 652256.0 40 31.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.745526944823744 20.530842033790066 13.08566993071723 24.63796109066896 2 15.445868676578723 20.406583750952244 39.80836183581709 24.33918573665195 3 17.424289670884033 26.943025723209864 30.189470068562805 25.443214537343295 4 12.087145592927744 17.10972983184088 39.13019057945352 31.672933995777854 5 12.968664441749505 38.42905331652361 35.2370113690781 13.365270872648786 6 30.531937293763246 38.31746066955206 17.952562939145444 13.198039097539246 7 27.031097758609445 32.22615544956102 23.480942336858476 17.261804454971063 8 26.458590018061667 35.17321553107936 19.97060985725639 18.397584593602584 9 26.82776087901173 14.348260806588451 20.480716783578536 38.34326153082128 10-11 25.261080339732462 25.79178790270735 28.38216920420786 20.564962553352327 12-13 25.66107764693175 23.517764466597328 28.37784136437427 22.44331652209665 14-15 23.063128856064623 24.810559343001877 25.479516182888112 26.646795618045395 16-17 22.541240763722627 27.431042334293277 26.53401206325653 23.493704838727567 18-19 22.192856423959366 27.40990141688603 28.147513885959828 22.249728273194773 20-21 23.37974119858567 26.44583072778413 27.454257674488648 22.720170399141548 22-23 23.525012410520464 26.2710921169032 27.21084025312144 22.993055219454895 24-25 23.096159129371234 26.486449665962265 27.683120170506808 22.734271034159693 26-27 23.105451086661212 26.617862188296783 27.14624388516275 23.130442839879255 28-29 22.568114383338763 26.97698273308726 27.404559536319912 23.05034334725406 30-31 23.283483001996647 26.54350277250881 27.314829193936234 22.858185031558307 32-33 23.36646477352666 26.884538552710286 26.758714050408276 22.990282623354783 34-35 23.318617183613235 26.65372173669939 27.521884332768604 22.505776746918777 36-37 23.240032627928624 26.47941367424326 27.306733969721037 22.97381972810708 38-39 23.225462668579432 26.285456328935624 27.729563113438978 22.75951788904597 40-41 22.9198923751762 25.82274862272058 28.14293090092668 23.114428101176536 42-43 22.84234387199105 26.006974684720625 27.70096562259251 23.44971582069581 44-45 22.75582940703992 26.618433811527236 27.560015805194315 23.06572097623853 46-47 22.85875658557981 26.270530728332254 27.29364886639805 23.57706381968989 48-49 23.11881627796177 26.086495875118242 27.481116295608853 23.31357155131113 50-51 22.797873699410438 26.21693993308237 27.137553515160246 23.847632852346944 52-53 22.359608308512488 26.467656205757446 27.27328698508622 23.89944850064385 54-55 22.398163826562133 26.37473577856257 27.165324479155974 24.061775915719323 56-57 22.97081783966996 26.065740318707693 27.018290615308043 23.945151226314305 58-59 22.373207080672934 26.239797238853175 27.505967990969964 23.881027689503927 60-61 22.517064102059 26.70590945253504 26.846875663187824 23.930150782218142 62-63 22.616854102445966 26.283063844868735 26.593231023852553 24.50685102883274 64-65 21.992512492028695 26.246926831281353 26.89399424606828 24.866566430621674 66-67 22.41144039768242 26.038940064753334 26.796505110960872 24.753114426603375 68-69 22.0968761241269 26.137502296777264 26.900236276917582 24.86538530217825 70-71 22.127803668772785 25.838638653741313 26.91924881606646 25.11430886141944 72-73 22.401875264940294 25.969474986510534 26.894161375243968 24.734488373305204 74-75 22.277780490354758 26.240015816520092 26.731270522372576 24.750933170752575 76-77 22.113156202189025 26.17678932452605 26.578954678066324 25.1310997952186 78-79 22.000155540009832 26.108257750739146 26.688985292239316 25.202601417011707 80-81 21.861364619346286 26.395057233292352 26.815044494228502 24.928533653132863 82-83 22.203052294026513 25.767236320342164 27.267076627667407 24.76263475796392 84-85 22.439083890425625 25.92422584299173 26.528066042584186 25.108624223998454 86-87 21.628050821906452 26.36984270872848 26.839606161940445 25.162500307424622 88-89 22.04565703802368 25.708705969837055 26.755663464425318 25.489973527713953 90-91 22.05098222090971 25.541070011749067 26.853766890769172 25.55418087657205 92-93 22.188962113010756 25.662325066462095 26.647989289484546 25.500723531042603 94-95 21.41232109878315 25.512925237976575 27.166203400716004 25.908550262524273 96-97 21.564058743302546 25.336777014699486 27.079082791339438 26.020081450658534 98-99 21.306236409893447 25.810335747526388 26.91831736190324 25.965110480676927 100 21.588335985506284 25.380490065268496 26.728356851060465 26.302817098164756 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 386.0 1 304.0 2 387.0 3 579.0 4 509.5 5 536.5 6 903.0 7 1736.5 8 2868.0 9 3579.5 10 3656.0 11 3856.0 12 4632.0 13 5646.0 14 6886.5 15 8344.0 16 9901.0 17 11508.5 18 13112.5 19 14649.0 20 16254.0 21 18259.5 22 21017.0 23 24872.5 24 30066.0 25 37314.0 26 48515.5 27 62896.5 28 85155.0 29 124079.5 30 171965.0 31 218063.0 32 283021.0 33 346227.5 34 402301.0 35 469074.5 36 539004.5 37 638062.5 38 693060.0 39 679547.0 40 668225.5 41 668917.5 42 700281.0 43 748884.0 44 805136.5 45 908613.0 46 994824.5 47 1058723.5 48 1123998.0 49 1128729.0 50 1099428.5 51 1093294.5 52 1078989.0 53 1042116.0 54 1001434.0 55 902663.0 56 797464.0 57 684945.5 58 566667.5 59 502220.0 60 416526.5 61 314805.0 62 241075.0 63 186498.0 64 133859.5 65 85477.0 66 57749.5 67 42734.5 68 31469.5 69 22251.5 70 16072.5 71 11400.5 72 8102.5 73 5848.5 74 4271.5 75 2793.0 76 1766.5 77 1140.5 78 712.0 79 441.0 80 288.0 81 190.0 82 119.0 83 83.0 84 61.5 85 45.5 86 30.5 87 18.0 88 14.5 89 11.0 90 9.0 91 6.0 92 2.0 93 1.5 94 0.5 95 0.5 96 1.5 97 1.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05000418805249599 2 0.0 3 4.138391794462964E-6 4 0.015576906714358598 5 0.004051485566779242 6 0.0 7 1.9864280613422227E-4 8 3.807320450905927E-4 9 0.0019698744941643712 10-11 8.173323794064355E-4 12-13 3.248637558653427E-4 14-15 4.345311384186113E-5 16-17 8.276783588925929E-5 18-19 0.0 20-21 1.8622763075083338E-5 22-23 6.207587691694446E-6 24-25 6.000668101971298E-5 26-27 2.0691958972314822E-5 28-29 0.0010221827732323522 30-31 8.752698645289169E-4 32-33 0.0138222285935063 34-35 0.022690802209040434 36-37 0.03050201672108928 38-39 0.020236735874923896 40-41 1.407053210117408E-4 42-43 0.0018477919362277137 44-45 0.009514162735470354 46-47 0.014165715112446728 48-49 0.005561998571758224 50-51 0.010269419237959846 52-53 0.01588728609894332 54-55 0.010031461709778224 56-57 0.003052063948416436 58-59 0.008071933195100012 60-61 0.0015891424490737781 62-63 9.642452881098707E-4 64-65 0.01932422048424481 66-67 0.045251245076555285 68-69 0.0549930193607211 70-71 0.048208126013699074 72-73 0.04117285996311203 74-75 0.028784584126387146 76-77 0.028956327385857364 78-79 0.012373791465444263 80-81 0.002089887856203797 82-83 0.027468575535747924 84-85 0.047837739948094635 86-87 0.04847712148033916 88-89 0.05951214320027466 90-91 0.034917680765781264 92-93 0.026520883814815908 94-95 0.007569118592072762 96-97 0.003645923170921872 98-99 0.022003829171159582 100 0.01879243713865632 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.4163976E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.2624014988718 #Duplication Level Percentage of deduplicated Percentage of total 1 65.55440361367118 29.67149736381078 2 15.368858848270808 13.912629195398438 3 6.9181069334303515 9.3939040089916 4 3.646994408222358 6.6028690067640285 5 2.3278869204920905 5.268287621964262 6 1.5116010822688961 4.1051217055070355 7 0.994040348459718 3.1494857350643537 8 0.7285444652184362 2.638053767559816 9 0.5170293230772106 2.1061789927029553 >10 2.324611712518634 17.290458119499572 >50 0.07945293078539209 2.447987692996422 >100 0.027045566797116997 2.067046473580989 >500 9.530725993028139E-4 0.2801207479411618 >1k 3.948510802198057E-4 0.32905285529452133 >5k 1.5184621654295326E-5 0.06782201816917657 >10k+ 6.0738486617181304E-5 0.6694846947548967 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 32287 0.13361625586782572 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25747 0.10655117353203794 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.2415175383388892E-5 2 0.0 0.0 0.0 2.0691958972314822E-5 1.2415175383388892E-5 3 0.0 4.138391794462964E-6 0.0 2.0691958972314822E-5 2.4830350766777784E-5 4 0.0 4.138391794462964E-6 0.0 4.5522309739092605E-5 2.896874256124075E-5 5 0.0 5.3799093328018536E-5 0.0 4.5522309739092605E-5 2.896874256124075E-5 6 0.0 5.3799093328018536E-5 0.0 4.5522309739092605E-5 2.896874256124075E-5 7 0.0 6.207587691694446E-5 0.0 4.5522309739092605E-5 2.896874256124075E-5 8 0.0 6.207587691694446E-5 0.0 4.5522309739092605E-5 2.896874256124075E-5 9 0.0 6.207587691694446E-5 0.0 7.03526605058704E-5 4.1383917944629644E-5 10-11 0.0 6.207587691694446E-5 0.0 8.690622768372226E-5 4.1383917944629644E-5 12-13 0.0 7.862944409479632E-5 0.0 1.1587497024496301E-4 7.449105230033335E-5 14-15 0.0 8.483703178649077E-5 0.0 1.4070532101174076E-4 9.932140306711113E-5 16-17 0.0 8.690622768372224E-5 0.0 1.7174325947021303E-4 1.1794416614219448E-4 18-19 0.0 1.034597948615741E-4 0.0 2.31749940489926E-4 1.2415175383388892E-4 20-21 4.138391794462964E-6 1.2001336203942596E-4 0.0 3.1658697227641676E-4 1.4277451690897228E-4 22-23 4.138391794462964E-6 1.2829014562835189E-4 0.0 6.766270583946946E-4 1.5932808408682412E-4 24-25 4.138391794462964E-6 1.3449773332004634E-4 0.0 0.0014567139116509636 1.8002004305913894E-4 26-27 4.138391794462964E-6 1.386361251145093E-4 0.0 0.0028223832038237417 1.9036602254529636E-4 28-29 4.138391794462964E-6 1.489821046006667E-4 0.0 0.010782579820473252 1.9657361023699078E-4 30-31 4.138391794462964E-6 1.6553567177851858E-4 0.0 0.044709115751480635 2.089887856203797E-4 32-33 4.138391794462964E-6 1.6760486767575006E-4 0.0 0.11770620861401286 2.2761154869546302E-4 34-35 4.138391794462964E-6 1.75881651264676E-4 0.0 0.21941132535473468 2.524418994622408E-4 36-37 4.138391794462964E-6 1.9243521844252782E-4 0.0 0.39465359508716613 2.524418994622408E-4 38-39 4.138391794462964E-6 2.1105798151761117E-4 0.0 0.6964644394614529 2.627878789483982E-4 40-41 4.138391794462964E-6 2.1519637331207414E-4 0.0 1.0365760998934943 2.689954666400927E-4 42-43 4.138391794462964E-6 2.1519637331207414E-4 0.0 1.3686944565745307 2.7520305433178713E-4 44-45 4.138391794462964E-6 2.1519637331207414E-4 0.0 1.7342303269958554 2.8968742561240747E-4 46-47 4.138391794462964E-6 2.193347651065371E-4 0.0 2.169742264269754 3.083101886874908E-4 48-49 4.138391794462964E-6 2.2347315690100008E-4 0.0 2.657408284133373 3.3107134355703715E-4 50-51 4.138391794462964E-6 2.2347315690100008E-4 0.0 3.1767061016779685 3.352097353515001E-4 52-53 4.138391794462964E-6 2.3588833228438896E-4 0.0 3.6648811437323063 3.683168697072038E-4 54-55 4.138391794462964E-6 3.331405394542686E-4 0.0 4.1593962020157615 3.9314722047398157E-4 56-57 4.138391794462964E-6 3.724552615016668E-4 0.0 4.722964465781624 4.0763159175460196E-4 58-59 4.138391794462964E-6 4.262543548296853E-4 0.0 5.361919743671323 4.2004676713799086E-4 60-61 4.138391794462964E-6 4.303927466241483E-4 0.0 6.0153490468621555 4.262543548296853E-4 62-63 4.138391794462964E-6 4.303927466241483E-4 0.0 6.662881141745879 4.759150563632409E-4 64-65 4.138391794462964E-6 4.510847055964631E-4 0.0 7.284827629360334 4.8005344815770385E-4 66-67 4.138391794462964E-6 4.759150563632409E-4 0.0 7.945888127020156 4.8419183995216677E-4 68-69 4.138391794462964E-6 4.862610358493983E-4 0.0 8.661101550506423 4.883302317466297E-4 70-71 4.138391794462964E-6 5.028146030272502E-4 0.0 9.414332310212526 5.048837989244816E-4 72-73 8.276783588925928E-6 5.172989743078705E-4 0.0 10.168022431407811 5.524753045608058E-4 74-75 8.276783588925928E-6 5.172989743078705E-4 0.0 10.870936554480934 5.731672635331205E-4 76-77 8.276783588925928E-6 5.172989743078705E-4 0.0 11.613306104922469 5.793748512248149E-4 78-79 8.276783588925928E-6 5.214373661023335E-4 0.0 12.394448248086324 5.917900266082038E-4 80-81 8.276783588925928E-6 5.359217373829539E-4 0.0 13.217309518930163 5.979976142998983E-4 82-83 8.276783588925928E-6 5.400601291774168E-4 0.0 14.038823743244903 6.124819855805187E-4 84-85 8.276783588925928E-6 5.421293250746483E-4 0.0 14.806253325197808 6.124819855805187E-4 86-87 8.276783588925928E-6 5.545445004580372E-4 0.0 15.570968122133543 6.207587691694446E-4 88 8.276783588925928E-6 5.669596758414261E-4 0.0 16.151551383762342 6.290355527583706E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 31380 0.0 44.079563 1 GTATCAA 53015 0.0 39.127697 1 CAACGCA 62060 0.0 31.935822 5 ATCAACG 64165 0.0 30.9354 3 TATCAAC 70270 0.0 29.283886 2 AACGCAG 69975 0.0 29.202791 6 TCAACGC 72405 0.0 27.642435 4 GTACATG 89525 0.0 27.151495 1 TAGGCAT 19160 0.0 26.902609 5 TACATGG 89985 0.0 26.507895 2 ACGCAGA 76360 0.0 25.831785 7 ACATGGG 89995 0.0 25.570648 3 CTAGGCA 20980 0.0 25.064657 4 CGCAGAG 78595 0.0 24.97764 8 CTAACGC 6630 0.0 24.878468 3 ATTTAGG 21780 0.0 24.436337 1 AGGGCTA 21710 0.0 24.327066 5 TCTAACG 6955 0.0 24.053404 2 GCTATTG 21730 0.0 23.828667 8 TAACGCC 6995 0.0 23.715057 4 >>END_MODULE