FastQCFastQC Report
Mon 6 Jun 2016
ERR1211416_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211416_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14741317
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT267850.18170018323328913No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197900.1342485206715248No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG149700.047.097862
GGTATCA174450.046.4519771
GTATCAA312100.040.018671
ACCTGGG178700.038.4845733
GTACCTG219700.032.660031
CAACGCA375900.032.288725
ATCAACG378950.031.9296053
TCAACGC397450.030.5732774
TATCAAC402750.030.501142
AACGCAG404150.030.4857756
GTACATG453400.029.1161081
CCTGGGG174950.028.6691254
TACATGG456100.028.2439792
ACATGGG450850.027.598713
ACGCAGA448450.027.0132987
CGCAGAG454550.026.4425138
CATGGGG334650.025.3261324
GTACTAG59900.025.247881
TGGGGGG281600.024.205176
ATGGGGG256500.023.4212685
Produced by FastQC (version 0.11.5)