##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211416_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 14741317 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.0716231121005 31.0 31.0 34.0 28.0 34.0 2 31.119831152128402 31.0 31.0 34.0 27.0 34.0 3 31.1892117915923 31.0 31.0 34.0 27.0 34.0 4 34.807336617209984 37.0 35.0 37.0 32.0 37.0 5 34.860333238882255 37.0 35.0 37.0 32.0 37.0 6 34.913177635349676 37.0 35.0 37.0 32.0 37.0 7 34.829025656255816 37.0 35.0 37.0 32.0 37.0 8 34.82601656283492 37.0 35.0 37.0 32.0 37.0 9 36.3625202551441 39.0 37.0 39.0 32.0 39.0 10-11 36.27563849959946 39.0 36.0 39.0 31.0 39.0 12-13 36.17372308050902 39.0 35.0 39.0 30.5 39.0 14-15 37.32115777036746 40.0 37.0 41.0 31.0 41.0 16-17 37.31692202263882 40.0 37.0 41.0 31.0 41.0 18-19 37.30335325534347 40.0 37.0 41.0 31.0 41.0 20-21 37.28388518474978 40.0 37.0 41.0 31.0 41.0 22-23 37.15529341781335 40.0 36.0 41.0 30.5 41.0 24-25 37.06048095974057 40.0 36.0 41.0 30.0 41.0 26-27 36.92831848063507 39.5 36.0 41.0 30.0 41.0 28-29 36.80382095439641 39.0 36.0 41.0 30.0 41.0 30-31 36.659271047491885 39.0 36.0 41.0 30.0 41.0 32-33 36.48664098329884 39.0 35.0 41.0 29.5 41.0 34-35 36.371603805820065 39.0 35.0 41.0 29.0 41.0 36-37 36.24763879645218 39.0 35.0 40.0 28.5 41.0 38-39 36.10119017859802 39.0 35.0 40.0 28.0 41.0 40-41 35.95466551597798 38.5 35.0 40.0 27.0 41.0 42-43 35.771660869920915 38.0 34.5 40.0 27.0 41.0 44-45 35.659986485603696 38.0 34.0 40.0 27.0 41.0 46-47 35.47143623598896 38.0 34.0 40.0 26.0 41.0 48-49 35.26317495919801 38.0 34.0 40.0 25.5 41.0 50-51 34.636308818269086 37.5 33.5 39.5 24.5 40.5 52-53 34.96417948952593 38.0 33.5 39.5 26.0 40.5 54-55 35.383958366813495 38.0 34.0 40.0 26.0 41.0 56-57 35.319214626481475 38.0 34.0 40.0 26.0 41.0 58-59 35.12290190896783 38.0 34.0 40.0 26.0 41.0 60-61 34.9105037562112 38.0 33.5 40.0 26.0 41.0 62-63 34.64714574009907 37.0 33.0 40.0 25.0 41.0 64-65 34.45401133426545 37.0 33.0 40.0 25.0 41.0 66-67 34.27622511611412 36.5 33.0 40.0 25.0 41.0 68-69 33.96278331169461 36.0 33.0 39.0 24.5 41.0 70-71 33.61766346928161 35.5 32.0 39.0 24.0 41.0 72-73 33.25685856290859 35.0 32.0 38.5 23.0 40.0 74-75 32.908566988960345 35.0 32.0 37.5 23.0 40.0 76-77 32.55386791424402 35.0 31.0 37.0 22.5 39.0 78-79 32.19143469338594 35.0 31.0 36.5 21.5 39.0 80-81 31.831856712666855 35.0 31.0 36.0 20.5 38.5 82-83 31.497210595227006 34.5 30.5 36.0 20.0 37.0 84-85 31.194299193213197 34.0 30.0 35.0 20.0 37.0 86-87 30.901841402637228 34.0 30.0 35.0 19.0 36.5 88-89 30.633743681110715 34.0 29.5 35.0 18.5 36.0 90-91 30.375956741178555 34.0 29.0 35.0 18.0 36.0 92-93 30.132604705536146 34.0 29.0 35.0 17.5 35.5 94-95 29.904784898120027 34.0 29.0 35.0 17.0 35.0 96-97 29.66768620469935 34.0 29.0 35.0 15.0 35.0 98-99 29.414964551674725 34.0 27.5 35.0 14.0 35.0 100 28.212129350450844 32.0 25.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3257995079035503 1101 2 -0.31504255136470505 1101 3 -0.37662023725368243 1101 4 -0.2374749504898901 1101 5 -0.26496337440704565 1101 6 -0.2625616683796679 1101 7 -0.26608616562982235 1101 8 -0.25067464819687757 1101 9 -0.41192447661379106 1101 10-11 -0.4056910902415538 1101 12-13 -0.42962921218697403 1101 14-15 -0.6082580741793393 1101 16-17 -0.615921162204593 1101 18-19 -0.6408806719185449 1101 20-21 -0.6040445552194456 1101 22-23 -0.5830942219374364 1101 24-25 -0.7255256169280315 1101 26-27 -0.7376792519018878 1101 28-29 -0.7615479715963573 1101 30-31 -0.7697304870110955 1101 32-33 -0.7677752978488428 1101 34-35 -0.8147430361515973 1101 36-37 -0.8082328745848244 1101 38-39 -0.8224708915138166 1101 40-41 -0.9929922387551997 1101 42-43 -1.001483060269294 1101 44-45 -1.0084650152058074 1101 46-47 -1.048289256932918 1101 48-49 -1.1068228138749632 1101 50-51 -0.9829413031302181 1101 52-53 -0.7982529136018854 1101 54-55 -0.778265312870289 1101 56-57 -0.7861977624893086 1101 58-59 -0.7681728004414694 1101 60-61 -0.846897835404036 1101 62-63 -0.9373584402323658 1101 64-65 -0.842654445840644 1101 66-67 -0.9119448048820189 1101 68-69 -0.9821002335738598 1101 70-71 -1.0135506807866506 1101 72-73 -1.0619931885850917 1101 74-75 -1.1029213964777043 1101 76-77 -0.9973991741839328 1101 78-79 -1.0832011660111789 1101 80-81 -1.1568118945456263 1101 82-83 -1.177843447515766 1101 84-85 -1.1589827798545187 1101 86-87 -1.2715010701232679 1101 88-89 -1.349516259338209 1101 90-91 -1.4405609411019178 1101 92-93 -1.5418449260553118 1101 94-95 -1.7040002076610463 1101 96-97 -1.7350863272248773 1101 98-99 -1.8834215423192866 1101 100 -1.866529165061305 1102 1 -0.3771291145197253 1102 2 -0.37070089447194476 1102 3 -0.39829844653020885 1102 4 -0.31674928775655076 1102 5 -0.274258325117529 1102 6 -0.27178419068974335 1102 7 -0.3766863661091975 1102 8 -0.3272479486675621 1102 9 -0.4581819215291887 1102 10-11 -0.48259698600474366 1102 12-13 -0.613338011371674 1102 14-15 -0.6932703584157096 1102 16-17 -0.6630620935759381 1102 18-19 -0.6815006157869448 1102 20-21 -0.7767324463954992 1102 22-23 -0.7084185355971897 1102 24-25 -0.7831295959177353 1102 26-27 -0.7880634546267942 1102 28-29 -0.8404892302067353 1102 30-31 -0.7690983526496069 1102 32-33 -0.8084188562349439 1102 34-35 -0.8834045123232528 1102 36-37 -0.8980173830440492 1102 38-39 -0.933500403983615 1102 40-41 -1.1465729794658372 1102 42-43 -1.0652994678318848 1102 44-45 -1.0021332426511904 1102 46-47 -1.2174360983023718 1102 48-49 -1.2767668568716317 1102 50-51 -1.0956170881821379 1102 52-53 -0.8663336443054277 1102 54-55 -0.9270052122078241 1102 56-57 -0.9221815481597417 1102 58-59 -0.9204000551934755 1102 60-61 -0.988525276953574 1102 62-63 -0.9572055942778306 1102 64-65 -1.0093764151825653 1102 66-67 -1.0640420640722894 1102 68-69 -1.0590797851885085 1102 70-71 -0.9504013181682893 1102 72-73 -0.9470371100061001 1102 74-75 -1.064758671926814 1102 76-77 -1.029861009654823 1102 78-79 -1.1041832318439901 1102 80-81 -1.1757617027773435 1102 82-83 -1.190594677743956 1102 84-85 -1.2146645734287276 1102 86-87 -1.2534858589405182 1102 88-89 -1.3212058068642456 1102 90-91 -1.3813854087818846 1102 92-93 -1.4196587448035274 1102 94-95 -1.4509792807162611 1102 96-97 -1.617259179119916 1102 98-99 -1.6448888470308276 1102 100 -1.5772769912835791 1103 1 -0.3788676077661641 1103 2 -0.44104825348542676 1103 3 -0.46690435334892655 1103 4 -0.32179857518097776 1103 5 -0.23662326257635868 1103 6 -0.305554866721252 1103 7 -0.3919425048392924 1103 8 -0.31413418208681065 1103 9 -0.432661682275878 1103 10-11 -0.5180929277311037 1103 12-13 -0.5733771394283096 1103 14-15 -0.7024856521038458 1103 16-17 -0.739608841123335 1103 18-19 -0.7717535745247517 1103 20-21 -0.6618796392213397 1103 22-23 -0.650799619089689 1103 24-25 -0.6300245106340583 1103 26-27 -0.8224627785269831 1103 28-29 -0.7728611739405906 1103 30-31 -0.7988774424799132 1103 32-33 -0.9329606168901279 1103 34-35 -0.8092121052232386 1103 36-37 -0.8850969831514277 1103 38-39 -0.9184889983488986 1103 40-41 -0.895056635404849 1103 42-43 -0.8980855497155531 1103 44-45 -0.9070250882948514 1103 46-47 -0.9739394515276274 1103 48-49 -0.9558471460280131 1103 50-51 -0.9194057801155182 1103 52-53 -0.7315912484751479 1103 54-55 -0.7558514427041416 1103 56-57 -0.7878486918291472 1103 58-59 -0.7431807457095587 1103 60-61 -0.70655810331575 1103 62-63 -0.7638650279337185 1103 64-65 -0.6685924772616261 1103 66-67 -0.7858723438957114 1103 68-69 -0.7655156743920131 1103 70-71 -0.8376369437376923 1103 72-73 -0.8083147064680531 1103 74-75 -0.8706154528399175 1103 76-77 -0.8607221143750223 1103 78-79 -0.8789686227138134 1103 80-81 -0.8872651243040259 1103 82-83 -0.9528818685432121 1103 84-85 -0.9227500353881588 1103 86-87 -1.0213635498642617 1103 88-89 -0.9895939304082226 1103 90-91 -0.9840861192756627 1103 92-93 -1.1521362680239022 1103 94-95 -1.0946266373719382 1103 96-97 -1.1904521985000223 1103 98-99 -1.2830304838639641 1103 100 -1.3524274672555627 1104 1 -0.4797182538715177 1104 2 -0.44066108912487323 1104 3 -0.4497728615790564 1104 4 -0.26647468415605857 1104 5 -0.31480106349017944 1104 6 -0.32467205391813536 1104 7 -0.33300192731680767 1104 8 -0.3819972999469954 1104 9 -0.4719171553258761 1104 10-11 -0.45565787660803636 1104 12-13 -0.5468024135196217 1104 14-15 -0.7731614680035435 1104 16-17 -0.8005564884832097 1104 18-19 -0.7802363228004836 1104 20-21 -0.8123864182928457 1104 22-23 -0.7893159890473527 1104 24-25 -0.7550883047616495 1104 26-27 -0.7845825542099973 1104 28-29 -0.8762281054759669 1104 30-31 -0.9412889147760879 1104 32-33 -0.8791977160304398 1104 34-35 -0.8545251219735661 1104 36-37 -1.0007974171524197 1104 38-39 -0.9978956604212073 1104 40-41 -0.9568864419797123 1104 42-43 -0.94524897145957 1104 44-45 -0.9634330406840732 1104 46-47 -0.8943442188872126 1104 48-49 -0.9295261705142437 1104 50-51 -0.9235203194721464 1104 52-53 -0.7530764743810678 1104 54-55 -0.7557966209789697 1104 56-57 -0.8106925323037615 1104 58-59 -0.7211234107879037 1104 60-61 -0.8097148935652356 1104 62-63 -0.7702413170958877 1104 64-65 -0.8589404124774163 1104 66-67 -0.7696169720017494 1104 68-69 -0.8748999297080147 1104 70-71 -0.764656975636484 1104 72-73 -0.818341493188619 1104 74-75 -0.8791365387939152 1104 76-77 -0.8890615166859988 1104 78-79 -0.8529255087612988 1104 80-81 -0.8507898430994629 1104 82-83 -0.9397890375504794 1104 84-85 -0.9197518153187971 1104 86-87 -1.0015968508030255 1104 88-89 -1.006101666997889 1104 90-91 -1.0277999871686454 1104 92-93 -1.0362075054348239 1104 94-95 -1.0964553965891461 1104 96-97 -1.1328389938026895 1104 98-99 -1.2194186854203828 1104 100 -1.1259042489414206 1105 1 -0.5161836757011322 1105 2 -0.5320955142931219 1105 3 -0.5433706128449955 1105 4 -0.39583376615216537 1105 5 -0.4500158089315889 1105 6 -0.3777485989629099 1105 7 -0.4246458542436713 1105 8 -0.4517160139789951 1105 9 -0.62169963959483 1105 10-11 -0.5283642034718596 1105 12-13 -0.6007623399741178 1105 14-15 -0.7992090856214276 1105 16-17 -0.7825368035774218 1105 18-19 -0.7340843550019187 1105 20-21 -0.7988855507077801 1105 22-23 -0.7371164968296142 1105 24-25 -0.7453311342653919 1105 26-27 -0.8278933036927683 1105 28-29 -0.8370916450489148 1105 30-31 -0.8604310469302803 1105 32-33 -0.8947808240498603 1105 34-35 -0.906374326581215 1105 36-37 -0.9221505239396066 1105 38-39 -1.0125135479648577 1105 40-41 -0.8896855104837798 1105 42-43 -0.8496593936642114 1105 44-45 -0.888914864034696 1105 46-47 -1.0322112990063914 1105 48-49 -1.0386430519583456 1105 50-51 -1.0277081641957437 1105 52-53 -0.8500513755792767 1105 54-55 -0.8182767953263976 1105 56-57 -0.8040231716178567 1105 58-59 -0.7991341112269552 1105 60-61 -0.8356729511345762 1105 62-63 -0.8332504861741867 1105 64-65 -0.8745070851564165 1105 66-67 -0.8426308518002017 1105 68-69 -0.8859134382742582 1105 70-71 -0.8588346879308517 1105 72-73 -0.7679933737040869 1105 74-75 -0.7871089317750162 1105 76-77 -0.9055838806917897 1105 78-79 -0.8871795401912514 1105 80-81 -0.8240773113226112 1105 82-83 -0.940140393876252 1105 84-85 -0.8259523558266402 1105 86-87 -0.8559471542624735 1105 88-89 -0.9956751902839436 1105 90-91 -0.9810455120682136 1105 92-93 -0.9292999578800476 1105 94-95 -1.07808380844304 1105 96-97 -1.074117486934778 1105 98-99 -0.9685904809922086 1105 100 -1.032454527354414 1106 1 -0.48901835247170666 1106 2 -0.5646734328976208 1106 3 -0.5706699247493496 1106 4 -0.372908649604085 1106 5 -0.4538808722896377 1106 6 -0.4922639980402792 1106 7 -0.520199454150621 1106 8 -0.5093017759984022 1106 9 -0.6950824639422564 1106 10-11 -0.7246387571054953 1106 12-13 -0.706228781569628 1106 14-15 -0.8776581576181002 1106 16-17 -0.7977576880085167 1106 18-19 -0.8737484112494727 1106 20-21 -0.8798085555787196 1106 22-23 -0.7948724652266961 1106 24-25 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-0.8968504962585406 1106 90-91 -0.989324407809832 1106 92-93 -1.0132422371849685 1106 94-95 -0.9618217641097608 1106 96-97 -1.0347846655412702 1106 98-99 -1.159220831573652 1106 100 -1.1054735789895531 1107 1 -0.563003182819088 1107 2 -0.5436784112037323 1107 3 -0.5485176766897943 1107 4 -0.37936336406720983 1107 5 -0.41791558988202127 1107 6 -0.4624009680425658 1107 7 -0.4616612131798945 1107 8 -0.5130838589209077 1107 9 -0.6341574933468124 1107 10-11 -0.54508523201347 1107 12-13 -0.6810546803017772 1107 14-15 -0.8067831757801542 1107 16-17 -0.8952614958622505 1107 18-19 -0.8122606473914615 1107 20-21 -0.9104770765045984 1107 22-23 -0.8155871521633742 1107 24-25 -0.8445595542815099 1107 26-27 -0.9063675438072565 1107 28-29 -0.8982366171984992 1107 30-31 -0.9716429968957669 1107 32-33 -1.0228110674444153 1107 34-35 -1.0220743652368327 1107 36-37 -0.996913331894774 1107 38-39 -0.9878244277997581 1107 40-41 -0.9700097164301056 1107 42-43 -0.9769200500615725 1107 44-45 -0.9556354435325005 1107 46-47 -0.9065225338102891 1107 48-49 -0.9665382426592473 1107 50-51 -1.004478033563359 1107 52-53 -0.7762072690634128 1107 54-55 -0.6939741642665851 1107 56-57 -0.6655781669851848 1107 58-59 -0.7369537132848265 1107 60-61 -0.7527723746385746 1107 62-63 -0.7504794808474102 1107 64-65 -0.8039000167074235 1107 66-67 -0.7478576816119116 1107 68-69 -0.72316650223582 1107 70-71 -0.6029307014216556 1107 72-73 -0.6415800069943387 1107 74-75 -0.5851230724751488 1107 76-77 -0.6554158731576294 1107 78-79 -0.6893306692368633 1107 80-81 -0.6352433690658827 1107 82-83 -0.7192026072374169 1107 84-85 -0.7017164406179823 1107 86-87 -0.7251523663649451 1107 88-89 -0.7402980800301862 1107 90-91 -0.7516274264947818 1107 92-93 -0.7628767508120475 1107 94-95 -0.850332529656324 1107 96-97 -0.7870477142172838 1107 98-99 -0.8440777698869439 1107 100 -0.8566337932952415 1108 1 -0.510685605477903 1108 2 -0.45586507282774846 1108 3 -0.5386201715180228 1108 4 -0.3820429634553051 1108 5 -0.37450079826385263 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0.9813075554735846 2115 80-81 1.032242449752161 2115 82-83 1.0341378813587916 2115 84-85 1.0672326058254598 2115 86-87 0.9434486309171 2115 88-89 1.0573360864279486 2115 90-91 1.1645496318449702 2115 92-93 1.2519133965051203 2115 94-95 1.2987581332711606 2115 96-97 1.2903155990691424 2115 98-99 1.3548493290911416 2115 100 1.4213723661336282 2116 1 0.2956771540238634 2116 2 0.2799948058087267 2116 3 0.26682088120147895 2116 4 0.09121684605543123 2116 5 0.11577577611694068 2116 6 0.1600772425122159 2116 7 0.15083364740508642 2116 8 0.15107703691235486 2116 9 0.21048275226497282 2116 10-11 0.20417085581147632 2116 12-13 0.27140474836441086 2116 14-15 0.41314091030690747 2116 16-17 0.437301771984572 2116 18-19 0.42663925475670084 2116 20-21 0.4506383055298073 2116 22-23 0.41756860190214695 2116 24-25 0.2520923279959959 2116 26-27 0.32461211231198916 2116 28-29 0.4399681948327654 2116 30-31 0.5457980443328694 2116 32-33 0.417138865041089 2116 34-35 0.5380821680231378 2116 36-37 0.6438102990114771 2116 38-39 0.7327299215114067 2116 40-41 0.6451073554062319 2116 42-43 0.6578843418634435 2116 44-45 0.6083976361779335 2116 46-47 0.58576882480412 2116 48-49 0.5663483648147576 2116 50-51 0.4818171183091948 2116 52-53 0.2838649796978885 2116 54-55 0.3322782453077906 2116 56-57 0.3761595973070726 2116 58-59 0.3263470419119088 2116 60-61 0.4480670112183347 2116 62-63 0.19590278429514996 2116 64-65 0.024369847738348938 2116 66-67 0.20084285977159055 2116 68-69 0.2234490443404198 2116 70-71 0.06299053334190319 2116 72-73 0.1856144453242905 2116 74-75 0.3544386320229975 2116 76-77 0.5318954299267133 2116 78-79 0.566038296780448 2116 80-81 0.6225319813861603 2116 82-83 0.4563710604242175 2116 84-85 0.6183633487074403 2116 86-87 0.7376141185829397 2116 88-89 0.8019694101637533 2116 90-91 0.8124048181070798 2116 92-93 0.9065682606160124 2116 94-95 0.9723537373901152 2116 96-97 1.0080247954624504 2116 98-99 1.0096143044706167 2116 100 0.9285337966750937 2201 1 0.7697629506152417 2201 2 0.7909651398018767 2201 3 0.854584586612976 2201 4 0.7155633345239991 2201 5 0.6529866928710533 2201 6 0.6729416529793752 2201 7 0.734694894930783 2201 8 0.7563592079613599 2201 9 0.9702457926943566 2201 10-11 0.9760092819064212 2201 12-13 0.6307156740098918 2201 14-15 0.8911432323987967 2201 16-17 1.0549615819586293 2201 18-19 1.0449939104600716 2201 20-21 1.058894115525959 2201 22-23 1.0817758414762082 2201 24-25 1.0956632409797322 2201 26-27 1.0754005936528515 2201 28-29 1.089901696621105 2201 30-31 1.0862289302932453 2201 32-33 1.063707783613033 2201 34-35 1.0851316306117553 2201 36-37 0.5685458251821416 2201 38-39 0.8209460357101506 2201 40-41 0.8965139119659682 2201 42-43 1.0196478167050387 2201 44-45 1.0000628818284198 2201 46-47 0.9974930011982863 2201 48-49 0.9726916473995502 2201 50-51 1.0826444615470479 2201 52-53 1.0510274874644736 2201 54-55 1.0450900559033585 2201 56-57 0.995979948788964 2201 58-59 0.9541104016467443 2201 60-61 0.8990938580003629 2201 62-63 0.8693969392413265 2201 64-65 0.8273107097668557 2201 66-67 0.8052152686443677 2201 68-69 0.22855995398499118 2201 70-71 0.6296330293697423 2201 72-73 0.7320663594731727 2201 74-75 0.6937171228628287 2201 76-77 0.679560776072897 2201 78-79 0.6483082556431441 2201 80-81 0.5530856288896899 2201 82-83 0.5929741564398299 2201 84-85 0.5532913116509022 2201 86-87 0.5250243072422194 2201 88-89 0.5366839534046086 2201 90-91 0.4524574732156701 2201 92-93 0.39290413431645277 2201 94-95 0.43026275707426365 2201 96-97 0.35744110081682123 2201 98-99 0.3184049065556174 2201 100 0.285824981688549 2202 1 0.79238421660909 2202 2 0.8152334063552225 2202 3 0.8532368408959705 2202 4 0.7661442987114029 2202 5 0.770873894800026 2202 6 0.7958627074197508 2202 7 0.8207757778088762 2202 8 0.8377566855740639 2202 9 0.9972909010417226 2202 10-11 0.9995008056996753 2202 12-13 1.0494848375777366 2202 14-15 1.2736218290933365 2202 16-17 1.2656228431045164 2202 18-19 1.2276786145102179 2202 20-21 1.259995403347574 2202 22-23 0.7616291977597385 2202 24-25 0.9412909791053821 2202 26-27 1.177726801245413 2202 28-29 1.212814506319475 2202 30-31 1.2083148421841017 2202 32-33 1.2322875961112842 2202 34-35 1.2020202093883867 2202 36-37 1.1745583989081183 2202 38-39 1.1932365735524115 2202 40-41 1.1485449208529772 2202 42-43 0.47729846151887756 2202 44-45 0.8689881413670761 2202 46-47 1.0369852239755062 2202 48-49 1.0095371587336288 2202 50-51 1.1491430616014213 2202 52-53 1.094528813526459 2202 54-55 1.0783905016903361 2202 56-57 1.031380902589376 2202 58-59 0.9975613581002563 2202 60-61 0.9610398063001782 2202 62-63 1.007281383221077 2202 64-65 0.9933391486974372 2202 66-67 0.9519501250552551 2202 68-69 0.9330592005584819 2202 70-71 0.9205579379238173 2202 72-73 0.828432017867037 2202 74-75 0.8264238110259043 2202 76-77 0.7971015066656193 2202 78-79 0.7854584511474556 2202 80-81 0.7620641678579716 2202 82-83 0.7045618418737583 2202 84-85 0.7073675315014327 2202 86-87 0.6767384299647254 2202 88-89 0.6736095146720906 2202 90-91 0.6814304854641584 2202 92-93 0.6375627337110998 2202 94-95 0.601307420925739 2202 96-97 0.6425894845302089 2202 98-99 0.5789143797436545 2202 100 0.5938313556819885 2203 1 -0.10285498141483984 2203 2 0.061600097147216815 2203 3 0.4561532637789867 2203 4 0.4737434976961197 2203 5 0.5021753150220079 2203 6 0.598405217925297 2203 7 0.6382961140105081 2203 8 0.6802547810025033 2203 9 0.8530433945084681 2203 10-11 0.8335884709797057 2203 12-13 0.9211314962348567 2203 14-15 1.1104311660335284 2203 16-17 1.086244813559226 2203 18-19 1.0724136392450987 2203 20-21 1.0686208276368632 2203 22-23 1.110854502041768 2203 24-25 1.0684648230194966 2203 26-27 1.0761873877552617 2203 28-29 1.0363819950752102 2203 30-31 1.0654444432369772 2203 32-33 1.0631767632647708 2203 34-35 1.0412909869687184 2203 36-37 1.022305796446858 2203 38-39 1.0783549659202833 2203 40-41 1.0311259427547483 2203 42-43 0.9759383149299268 2203 44-45 0.9793442369553489 2203 46-47 0.9682006533391245 2203 48-49 0.9659699199930358 2203 50-51 1.065864384146792 2203 52-53 1.0407964801932792 2203 54-55 0.9443169384729941 2203 56-57 0.9527254319181182 2203 58-59 0.891606582743016 2203 60-61 0.9087614092250718 2203 62-63 0.8725160484930896 2203 64-65 0.8983061716243483 2203 66-67 0.8890574369936317 2203 68-69 0.8663098816042734 2203 70-71 0.8382511886781288 2203 72-73 0.764259932065471 2203 74-75 0.7857728894217431 2203 76-77 0.7526196165994676 2203 78-79 0.7399816178830818 2203 80-81 0.6768090300467549 2203 82-83 0.6828380864015635 2203 84-85 0.6877856202708585 2203 86-87 0.7122640864282062 2203 88-89 0.7176072515440666 2203 90-91 0.6711012041889646 2203 92-93 0.7061784723683786 2203 94-95 0.6849660760799274 2203 96-97 0.6639041706614996 2203 98-99 0.6659849636264532 2203 100 0.6751717963944834 2204 1 0.7389229397389485 2204 2 0.8060621028987143 2204 3 0.8555134370565618 2204 4 0.6776113248518243 2204 5 0.7275613678950634 2204 6 0.6958684657053027 2204 7 0.7686841523933481 2204 8 0.7435438605183649 2204 9 0.9598398345001442 2204 10-11 0.9529204830444797 2204 12-13 0.9820246183230239 2204 14-15 1.2133546277030476 2204 16-17 1.1716400787918886 2204 18-19 1.1582579689661898 2204 20-21 1.1268912958869137 2204 22-23 1.1870605653817279 2204 24-25 1.2050889965080742 2204 26-27 1.211244840168682 2204 28-29 1.176546733210536 2204 30-31 1.2018266471629744 2204 32-33 1.2568935575439681 2204 34-35 1.2325045679683342 2204 36-37 1.1816640794605036 2204 38-39 1.231875466942796 2204 40-41 1.2247514588154047 2204 42-43 1.1926570173950637 2204 44-45 1.2257550969217306 2204 46-47 1.1761311727829735 2204 48-49 1.1710584496077914 2204 50-51 1.2433877089553391 2204 52-53 1.1834991202618923 2204 54-55 1.0855497546269675 2204 56-57 1.1024237561658978 2204 58-59 1.143516825752954 2204 60-61 1.1257260827059312 2204 62-63 1.1398635088722315 2204 64-65 1.1447728152291248 2204 66-67 1.0848279686469908 2204 68-69 1.0842653768787827 2204 70-71 1.08368416676133 2204 72-73 1.0366777689881062 2204 74-75 1.0126596112137776 2204 76-77 0.94643937784911 2204 78-79 0.8928978769369351 2204 80-81 0.9165375083437155 2204 82-83 0.9111366166734634 2204 84-85 0.896852524229331 2204 86-87 0.8478356804842804 2204 88-89 0.8281884237263704 2204 90-91 0.8155992681055828 2204 92-93 0.8259546851834685 2204 94-95 0.8529434722656077 2204 96-97 0.819987934940066 2204 98-99 0.8098763114364829 2204 100 0.82157653582831 2205 1 0.8237252026240398 2205 2 0.8644083612534743 2205 3 0.8937214283648593 2205 4 0.7518794242214426 2205 5 0.7534250597699099 2205 6 0.7598274233508278 2205 7 0.8531089646964176 2205 8 0.8733853417308239 2205 9 1.0148509755719672 2205 10-11 1.0533795757107853 2205 12-13 1.087709878693424 2205 14-15 1.3139812951750116 2205 16-17 1.3095106485866168 2205 18-19 1.262093208545494 2205 20-21 1.2594460767171967 2205 22-23 1.300325822881213 2205 24-25 1.333269292293238 2205 26-27 1.351691652454818 2205 28-29 1.3567697547593056 2205 30-31 1.3583512625909577 2205 32-33 1.338959697219579 2205 34-35 1.302703083903225 2205 36-37 1.355871557345317 2205 38-39 1.3521047047419401 2205 40-41 1.3769985798314863 2205 42-43 1.3654768902524168 2205 44-45 1.3633328951268169 2205 46-47 1.3598568644057707 2205 48-49 1.3648891749921717 2205 50-51 1.4377131509616845 2205 52-53 1.3906044527279846 2205 54-55 1.3017055850628765 2205 56-57 1.2479312472297224 2205 58-59 1.2711880404179041 2205 60-61 1.2817595808919648 2205 62-63 1.2555851726236753 2205 64-65 1.2517672207468777 2205 66-67 1.2674936043279317 2205 68-69 1.2241104873009903 2205 70-71 1.2168645847823143 2205 72-73 1.0279375331732368 2205 74-75 1.1282775533812952 2205 76-77 1.1346961275479615 2205 78-79 1.11817093146405 2205 80-81 1.0891306202597022 2205 82-83 1.109050453437657 2205 84-85 1.1024941858709028 2205 86-87 1.0438447902839627 2205 88-89 1.051198800100547 2205 90-91 1.0907097126599474 2205 92-93 1.0267966616243918 2205 94-95 1.0580931691047972 2205 96-97 1.1047614214973542 2205 98-99 1.0852264339733573 2205 100 1.1340210268115278 2206 1 0.7355925853282379 2206 2 0.7987660812728308 2206 3 0.8125461961405414 2206 4 0.702863723837261 2206 5 0.6901794644128714 2206 6 0.7066313842081513 2206 7 0.7832201291449579 2206 8 0.7935869432257334 2206 9 0.9398740849594773 2206 10-11 0.9683807075562996 2206 12-13 0.9892361692177971 2206 14-15 1.1916763229363738 2206 16-17 1.202995586221725 2206 18-19 1.2058290771007805 2206 20-21 1.1799109355388921 2206 22-23 1.218715771589082 2206 24-25 1.217864937858856 2206 26-27 1.2418672893996572 2206 28-29 1.251204896651572 2206 30-31 1.2691138884781523 2206 32-33 1.3075308602037907 2206 34-35 1.2678652009989122 2206 36-37 1.2559601870786778 2206 38-39 1.318329160696635 2206 40-41 1.3188124809102817 2206 42-43 1.3406913634276734 2206 44-45 1.34224445032455 2206 46-47 1.3848155628940262 2206 48-49 1.3912773446656814 2206 50-51 1.4532681521439272 2206 52-53 1.275232156750313 2206 54-55 1.217755196434581 2206 56-57 1.260064813119861 2206 58-59 1.2029232666841096 2206 60-61 1.2206043649040694 2206 62-63 1.1535474447198908 2206 64-65 1.1748434438575046 2206 66-67 1.1647113922636194 2206 68-69 1.1556514729179455 2206 70-71 1.1492861280092228 2206 72-73 1.1305509833617506 2206 74-75 1.1343760098016062 2206 76-77 1.1180585584805343 2206 78-79 1.0876171223244242 2206 80-81 1.0852859153437961 2206 82-83 1.09642621116868 2206 84-85 1.1071074537881884 2206 86-87 1.0884823414322504 2206 88-89 1.0917323788140685 2206 90-91 1.1176760545002544 2206 92-93 1.0905784311700089 2206 94-95 1.0911129595320865 2206 96-97 1.1489554739956667 2206 98-99 1.1776590842655743 2206 100 1.2639108339162135 2207 1 0.7880837681701358 2207 2 0.8122947794516193 2207 3 0.8656393156711708 2207 4 0.7021301969610789 2207 5 0.7332153502974563 2207 6 0.7732474429559062 2207 7 0.8447662985110966 2207 8 0.7957876594819808 2207 9 0.9969795732374251 2207 10-11 1.0106985020425796 2207 12-13 1.0670627458493627 2207 14-15 1.259833629015965 2207 16-17 1.2717642603330575 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1.543300467336202 2310 50-51 1.446036690170601 2310 52-53 1.3294116406772432 2310 54-55 1.3096097407364198 2310 56-57 1.2435770963415962 2310 58-59 1.2758200627575391 2310 60-61 1.2926594359236034 2310 62-63 1.2749278403534348 2310 64-65 1.2313336305123315 2310 66-67 1.2383503593988294 2310 68-69 1.284471339151203 2310 70-71 1.1990166486776843 2310 72-73 1.1225596987631477 2310 74-75 1.10655952576073 2310 76-77 1.16001737297902 2310 78-79 1.1449529164478136 2310 80-81 1.2023175625475346 2310 82-83 1.23656177765454 2310 84-85 1.2670990208944062 2310 86-87 1.298851367528016 2310 88-89 1.3609369210946767 2310 90-91 1.440524312646133 2310 92-93 1.42830763883887 2310 94-95 1.4954395699378153 2310 96-97 1.530820023259455 2310 98-99 1.547969732680965 2310 100 1.6552407101451436 2311 1 0.6914231892242846 2311 2 0.7471777662968151 2311 3 0.8004932120089769 2311 4 0.7279482678751137 2311 5 0.690328656131804 2311 6 0.6887094460594767 2311 7 0.733228981448363 2311 8 0.7557794109859586 2311 9 0.9577014118797464 2311 10-11 0.8193935605532445 2311 12-13 0.9549776621734907 2311 14-15 1.2142730983286967 2311 16-17 1.2127529994685986 2311 18-19 1.220277279004513 2311 20-21 1.2345453375408937 2311 22-23 1.2656304463223833 2311 24-25 1.3042717189764303 2311 26-27 1.326250531851727 2311 28-29 1.3653434082741995 2311 30-31 1.410024027513522 2311 32-33 1.3936913308634544 2311 34-35 1.4060117280286732 2311 36-37 1.4403877230365936 2311 38-39 1.479782921004869 2311 40-41 1.489885333785054 2311 42-43 1.5647271784933992 2311 44-45 1.5513677750323254 2311 46-47 1.5760938997583764 2311 48-49 1.6087418877784074 2311 50-51 1.5612271275650684 2311 52-53 1.4443693731353946 2311 54-55 1.4197209698303652 2311 56-57 1.3764789696893445 2311 58-59 1.386232033293247 2311 60-61 1.3737782565444974 2311 62-63 1.3643012910691112 2311 64-65 1.3406933689307436 2311 66-67 1.3784288357646304 2311 68-69 1.3758673587767802 2311 70-71 1.348486863661222 2311 72-73 1.3243533101884566 2311 74-75 1.325867072304753 2311 76-77 1.3354034668541104 2311 78-79 1.3489512128089913 2311 80-81 1.3637289430115231 2311 82-83 1.3855928571286036 2311 84-85 1.3281277139506926 2311 86-87 1.43297864915219 2311 88-89 1.4599420384932245 2311 90-91 1.4995066370979409 2311 92-93 1.5498401470711087 2311 94-95 1.6327318682695413 2311 96-97 1.6615604440976135 2311 98-99 1.7073555817225383 2311 100 1.8339711261649505 2312 1 0.6005961883105684 2312 2 0.6870427034935034 2312 3 0.7314071252824625 2312 4 0.5989082363354967 2312 5 0.6303939758736732 2312 6 0.49587765132591244 2312 7 0.6702932688833485 2312 8 0.7014205707937435 2312 9 0.8640036904895894 2312 10-11 0.9087821021193179 2312 12-13 0.9524385447467907 2312 14-15 1.1527924798174283 2312 16-17 1.1467921266453018 2312 18-19 1.1366595113210494 2312 20-21 1.1632585000903646 2312 22-23 1.1370564468853601 2312 24-25 1.2040784400078977 2312 26-27 1.239467421026383 2312 28-29 1.282952310879729 2312 30-31 1.1696816732568962 2312 32-33 1.2465404421403505 2312 34-35 1.3001905326804462 2312 36-37 1.3032732761259709 2312 38-39 1.3632005084392205 2312 40-41 1.41850435902046 2312 42-43 1.4686647586822303 2312 44-45 1.4508759186760543 2312 46-47 1.448668525782594 2312 48-49 1.5164416359098567 2312 50-51 1.4192843311887273 2312 52-53 1.2682527369833778 2312 54-55 1.2283792381813399 2312 56-57 1.223119213394419 2312 58-59 1.2220831114185344 2312 60-61 1.2132698265025894 2312 62-63 1.154498761545824 2312 64-65 1.1748394543492964 2312 66-67 1.1628007018337527 2312 68-69 1.1956897799728452 2312 70-71 1.190162204739032 2312 72-73 1.2456071252932972 2312 74-75 1.2422697149679252 2312 76-77 1.1952342912961385 2312 78-79 1.1914286528179332 2312 80-81 1.0290768653844822 2312 82-83 1.2242523800468241 2312 84-85 1.2724123559251268 2312 86-87 1.2938345608269373 2312 88-89 1.3412387473285605 2312 90-91 1.3626446059339976 2312 92-93 1.425261601079729 2312 94-95 1.4802500155532847 2312 96-97 1.5409562514088186 2312 98-99 1.3900188900312962 2312 100 1.5129734643095283 2313 1 0.609007988071852 2313 2 0.57584769071509 2313 3 0.6547258215984257 2313 4 0.5530917219314091 2313 5 0.5528436505639078 2313 6 0.6105028343344188 2313 7 0.6003673818851212 2313 8 0.6207018707378964 2313 9 0.8051887204787604 2313 10-11 0.7172430069181388 2313 12-13 0.8236774060303347 2313 14-15 1.0769028447590259 2313 16-17 1.022604896796345 2313 18-19 1.0352874471078977 2313 20-21 1.0849236174397276 2313 22-23 1.0565636691142473 2313 24-25 1.1073230267161094 2313 26-27 1.1174916245750097 2313 28-29 1.1314195582980773 2313 30-31 1.176118303976068 2313 32-33 1.2475871182054448 2313 34-35 1.2322329652641315 2313 36-37 1.218257355979624 2313 38-39 1.2531803073802479 2313 40-41 1.287604355951295 2313 42-43 1.3361991125312684 2313 44-45 1.2923488474129599 2313 46-47 1.3080954412423011 2313 48-49 1.3945168693915164 2313 50-51 1.3503142768250598 2313 52-53 1.2206194735674316 2313 54-55 1.162729067718189 2313 56-57 1.1460244434518359 2313 58-59 1.2113037807103524 2313 60-61 1.1921723367521722 2313 62-63 1.1819174076803591 2313 64-65 1.1715896537763868 2313 66-67 1.1619719026044137 2313 68-69 1.166114189044066 2313 70-71 1.198848774846624 2313 72-73 1.2053243734995576 2313 74-75 1.1556308477141286 2313 76-77 1.1806342887005243 2313 78-79 1.169742172522632 2313 80-81 1.1983396186130904 2313 82-83 1.2046400481804262 2313 84-85 1.2788202392756176 2313 86-87 1.2846123493560384 2313 88-89 1.3503947107170156 2313 90-91 1.4512085965633297 2313 92-93 1.4954797812764227 2313 94-95 1.5461253244436541 2313 96-97 1.5601175532710734 2313 98-99 1.5894814902412158 2313 100 1.73426806432796 2314 1 0.5681694952817757 2314 2 0.5742547956122337 2314 3 0.6176248398256412 2314 4 0.5571126374807207 2314 5 0.5876578505068224 2314 6 0.5732649706779469 2314 7 0.6055647819164349 2314 8 0.6162502987426919 2314 9 0.7977930217881095 2314 10-11 0.7725174398652257 2314 12-13 0.5071488176724372 2314 14-15 0.9217994239513772 2314 16-17 0.9486544104119119 2314 18-19 0.9881484490056422 2314 20-21 0.9933068039047015 2314 22-23 0.8912550855737109 2314 24-25 0.9893097367130039 2314 26-27 1.0999515499485781 2314 28-29 1.1068476884832776 2314 30-31 1.04208860451768 2314 32-33 1.1198587633432737 2314 34-35 1.1656294016508895 2314 36-37 1.211009256546987 2314 38-39 1.233260299825318 2314 40-41 1.2852655762313816 2314 42-43 1.397434492812259 2314 44-45 1.4118631324878734 2314 46-47 1.4159154835168835 2314 48-49 1.4362090052117011 2314 50-51 1.38800683754301 2314 52-53 1.2030546746389703 2314 54-55 1.1669493745237816 2314 56-57 1.1395555799609696 2314 58-59 1.1254882085003075 2314 60-61 1.1450427892397173 2314 62-63 1.1680920278044482 2314 64-65 1.1481406768568831 2314 66-67 1.2169991931195625 2314 68-69 1.1871239723518343 2314 70-71 1.2038346752990563 2314 72-73 1.2356270885114498 2314 74-75 1.2095802523337298 2314 76-77 1.2293551800548812 2314 78-79 1.2235914461551403 2314 80-81 1.22071697912029 2314 82-83 1.1000975248280618 2314 84-85 1.112102849885801 2314 86-87 1.2761230148346385 2314 88-89 1.388717991821725 2314 90-91 1.4205940498087593 2314 92-93 1.4803250996398525 2314 94-95 1.465057185999271 2314 96-97 1.5497914126156083 2314 98-99 1.5726255861358887 2314 100 1.5456128100780653 2315 1 0.44226924582707383 2315 2 0.45380510118674167 2315 3 0.4977045731766445 2315 4 0.4351426758778061 2315 5 0.3631708307579231 2315 6 -0.2548001882031059 2315 7 0.24562887340442074 2315 8 0.31180107567691806 2315 9 0.5390406183974363 2315 10-11 0.6135774787560493 2315 12-13 0.65963967404344 2315 14-15 0.7592974286236611 2315 16-17 0.6741074440983539 2315 18-19 0.7844684686803234 2315 20-21 0.8361120870694947 2315 22-23 0.7275627751756488 2315 24-25 0.8046020326386483 2315 26-27 0.876017400646127 2315 28-29 0.9417752036243385 2315 30-31 1.0297901647173333 2315 32-33 1.0269676559310525 2315 34-35 1.0336828215416602 2315 36-37 1.1151268536465864 2315 38-39 1.102742649991896 2315 40-41 1.1378598547482213 2315 42-43 1.1621879727817017 2315 44-45 1.1526984884344031 2315 46-47 1.1686350832445953 2315 48-49 1.1730017028203221 2315 50-51 1.0957771254777668 2315 52-53 0.9484705492705743 2315 54-55 0.807289822526883 2315 56-57 0.8002449969995808 2315 58-59 0.856621382595101 2315 60-61 0.7509527687451154 2315 62-63 0.9399421703562538 2315 64-65 0.9807891863716165 2315 66-67 0.9633427354069326 2315 68-69 1.0011084065054163 2315 70-71 1.0382661997200415 2315 72-73 1.0418984073742692 2315 74-75 1.0717701650729126 2315 76-77 1.0122269008266116 2315 78-79 0.9482947743055874 2315 80-81 0.9815090907948871 2315 82-83 1.147297860743155 2315 84-85 1.0766316086372 2315 86-87 1.0711133026861788 2315 88-89 1.2599688776892002 2315 90-91 1.2977259993658876 2315 92-93 1.3415390210312808 2315 94-95 1.4107884637755923 2315 96-97 1.4344836155138303 2315 98-99 1.5173060906016502 2315 100 1.5855370968285847 2316 1 0.02958415033166162 2316 2 -0.07872212771340514 2316 3 0.07153681456437155 2316 4 0.05500069720415013 2316 5 0.044761084438420085 2316 6 0.10607893951095804 2316 7 0.07504160674988469 2316 8 0.10096845261647047 2316 9 0.2572300018428564 2316 10-11 0.0998643440825262 2316 12-13 0.17968142295684686 2316 14-15 0.3435282512495945 2316 16-17 0.3864446031755264 2316 18-19 0.36214314861273067 2316 20-21 0.3371045352484572 2316 22-23 0.21295817036887144 2316 24-25 0.2651338669450709 2316 26-27 0.34890780193138937 2316 28-29 0.49823080883111714 2316 30-31 0.4488786093261936 2316 32-33 0.5448590420378139 2316 34-35 0.5901536106217833 2316 36-37 0.6541447124923678 2316 38-39 0.6507296485110174 2316 40-41 0.6513656336125777 2316 42-43 0.6987497910106768 2316 44-45 0.5450176102182596 2316 46-47 0.5305685368209225 2316 48-49 0.691290450137295 2316 50-51 0.40804814511344034 2316 52-53 0.14432482365813826 2316 54-55 0.15375129593054737 2316 56-57 0.2344412777489282 2316 58-59 0.3100432208980948 2316 60-61 0.40669677032044405 2316 62-63 0.47446247224343807 2316 64-65 0.46360219299175043 2316 66-67 0.45953359864697063 2316 68-69 0.4736215013799594 2316 70-71 0.3277342505903107 2316 72-73 0.4565190493729787 2316 74-75 0.5153899051163009 2316 76-77 0.5510814295417887 2316 78-79 0.6489818202503628 2316 80-81 0.7377067006906515 2316 82-83 0.7125687725899645 2316 84-85 0.7796339097207223 2316 86-87 0.8348181700805952 2316 88-89 0.8979331195556561 2316 90-91 0.8818793978352275 2316 92-93 0.931958077322335 2316 94-95 1.0060172030713694 2316 96-97 1.0684066808188355 2316 98-99 1.129553329118913 2316 100 0.995703857033952 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 79.0 1 841.0 2 409.0 3 303.0 4 252.0 5 222.0 6 257.0 7 1168.0 8 4006.0 9 8150.0 10 12917.0 11 19577.0 12 27832.0 13 35525.0 14 42415.0 15 50003.0 16 59738.0 17 72158.0 18 86411.0 19 100546.0 20 116952.0 21 134024.0 22 146689.0 23 154770.0 24 162501.0 25 174945.0 26 196368.0 27 226992.0 28 266286.0 29 313110.0 30 372329.0 31 456474.0 32 565684.0 33 723822.0 34 974550.0 35 1304312.0 36 1882632.0 37 2699804.0 38 2623261.0 39 721905.0 40 1098.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.64573958349834 18.97987624970792 11.978425382807977 24.395958783985762 2 15.58251619866889 20.38482640440095 40.65699447707186 23.3756629198583 3 17.721780605320152 27.357463115302753 30.081286415074292 24.8394698643028 4 11.689173138630146 16.8565571431838 39.60201130990333 31.85225840828272 5 12.465506832678546 38.14268641034857 35.42864827904142 13.963158477931465 6 29.64140642132735 38.089678292907585 19.30081015744664 12.968105128318424 7 26.72524843639932 32.544126385336426 23.901603696140096 16.82902148212416 8 26.08259138175765 35.3288995311243 20.973745987753347 17.614763099364712 9 27.215183645337433 14.770173481595766 20.669296962794522 37.34534591027228 10-11 24.707466468027047 25.924994903254127 29.49842794911561 19.869110679603217 12-13 25.174370574397624 23.99288544303716 29.078418727041704 21.75432525552351 14-15 22.59627105159043 25.36615082774035 26.60070658287476 25.436871537794463 16-17 22.12800146392147 27.63086639338459 27.454980055460886 22.786152087233056 18-19 21.82658352737932 27.48595614110049 28.84425713381284 21.843203197707346 20-21 22.945543725627143 26.905934659581575 28.23136755741865 21.917154057372628 22-23 22.67314092781677 26.845009552241418 28.04409566103882 22.43775385890299 24-25 22.223850658298677 26.805506448030126 28.54083578260297 22.42980711106823 26-27 22.331966907512875 26.917123108798886 28.132891984374748 22.618017999313498 28-29 22.14050789790042 27.44251881164902 28.15647332281483 22.260499967635727 30-31 22.90898685418631 26.945011686053522 28.096950057917162 22.049051401843002 32-33 22.6923854369938 27.102838870048558 27.934267267190037 22.270508425767606 34-35 22.72098095821285 27.125120911719915 28.194728437306754 21.959169692760483 36-37 22.581074824838833 27.091628802922134 28.071282158907557 22.256014213331472 38-39 22.50952067475437 27.166749921257576 28.163440364675463 22.160289039312588 40-41 22.58229968209474 26.67517046729875 28.411286573150647 22.33124327745587 42-43 22.335949271092236 26.877474580038985 28.05995299943461 22.726623149434168 44-45 22.10776132177536 27.253876941965956 28.22593725310398 22.412424483154712 46-47 22.11270877572512 26.854122809238095 28.008256796118513 23.024911618918274 48-49 22.259647425964687 26.851401529108653 28.125914363631075 22.763036681295578 50-51 22.170652940794284 27.132378025372304 27.876908889840003 22.820060143993413 52-53 21.9778804659918 27.25686634539528 27.767334879798426 22.997918308814494 54-55 21.857323911402176 27.08992288833654 28.01881897846133 23.03393422179995 56-57 22.20470057063267 26.933578368005424 27.88232309184277 22.979397969519137 58-59 21.995592933966797 27.03833251579606 28.10460699080054 22.861467559436598 60-61 22.222618787151173 27.185834317743566 27.731408895712068 22.86013799939319 62-63 22.17237005165374 26.8737156667042 27.807539989162994 23.14637429247907 64-65 21.834396690291076 26.668675618717142 27.91448486885566 23.582442822136123 66-67 21.916465647833462 26.641461162401413 27.72534444182361 23.716728747941517 68-69 21.746885330360836 26.76868806873076 27.83854810463061 23.645878496277785 70-71 21.843600994853293 26.448662303172643 27.937036690932654 23.77070001104141 72-73 21.847400108830158 26.579319989309724 27.806625039379334 23.76665486248078 74-75 21.863137988215353 26.58556621271225 27.721120037015606 23.830175762056783 76-77 21.748872123620938 26.403726312357012 27.800818656448918 24.046582907573132 78-79 21.5939298751351 26.400841951881553 27.95517446879911 24.050053704184236 80-81 21.646939588894593 26.491197858505405 27.804170790327504 24.057691762272494 82-83 21.999544593194233 26.26778245857389 27.9925509233624 23.74012202486947 84-85 22.09975928048332 26.345431861912306 27.52824863644175 24.026560221162622 86-87 21.625642771540786 26.502134158917766 27.838283456686124 24.033939612855324 88-89 21.843380059449167 25.991285597434118 27.863463752642787 24.301870590473936 90-91 22.144719724246453 25.86920012500697 27.8633442567425 24.122735894004073 92-93 22.26737201025705 25.89426739725053 27.718772977895963 24.11958761459645 94-95 21.894350724869756 25.78464793300502 27.847239279622087 24.473762062503138 96-97 21.939713025991622 25.66103718548966 27.830933175443594 24.568316613075126 98-99 21.933694285039884 25.739701454334842 27.618922859117784 24.707681401507493 100 22.218147011059937 25.44825211667448 27.477078464002624 24.85652240826296 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 427.0 1 375.0 2 838.5 3 1380.0 4 1188.0 5 1059.5 6 1402.5 7 2432.5 8 3820.0 9 4579.5 10 4688.5 11 4962.5 12 5668.0 13 6529.5 14 7552.5 15 8702.0 16 9732.5 17 10877.5 18 12220.0 19 13631.0 20 15581.0 21 17963.5 22 21650.5 23 27105.5 24 34493.5 25 44976.5 26 59472.5 27 77059.5 28 98091.5 29 125233.5 30 156173.0 31 187018.0 32 225400.5 33 262740.0 34 296180.0 35 332031.5 36 371557.5 37 418838.0 38 445638.5 39 450153.0 40 454862.0 41 460112.0 42 469744.0 43 486753.0 44 510130.0 45 541029.5 46 568092.5 47 587134.0 48 605325.0 49 613514.5 50 608732.0 51 603033.5 52 587593.5 53 563089.5 54 534144.0 55 485387.5 56 430995.5 57 375777.5 58 320902.0 59 273827.5 60 221334.0 61 170715.5 62 132192.0 63 101549.5 64 74473.0 65 51638.5 66 36390.5 67 26765.5 68 19697.0 69 14439.5 70 10993.5 71 8517.5 72 6470.5 73 4716.0 74 3459.0 75 2448.0 76 1684.0 77 1164.0 78 801.0 79 587.5 80 450.5 81 315.5 82 237.5 83 178.0 84 145.0 85 118.5 86 91.0 87 79.0 88 63.5 89 42.0 90 30.0 91 28.5 92 30.5 93 25.0 94 14.5 95 11.5 96 9.5 97 8.0 98 7.0 99 3.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21829799874733039 2 0.14645231494580843 3 0.023267934608556345 4 0.02211471335973577 5 0.0269853772224015 6 0.054323504473854 7 0.06088329828332163 8 0.05418783138575746 9 0.041339589943015266 10-11 0.04358497955101298 12-13 0.041895849604211076 14-15 0.020757982478770386 16-17 0.019140080903219163 18-19 0.018705927021310242 20-21 0.021887460937174065 22-23 0.024940105419346183 24-25 0.0220468768156875 26-27 0.01944195352423396 28-29 0.01928592947292294 30-31 0.01760019135332345 32-33 0.025289463621194767 34-35 0.025835547800783332 36-37 0.02444829047499623 38-39 0.023003372086768097 40-41 0.024373670276543132 42-43 0.03034328615279083 44-45 0.03977256577550025 46-47 0.04440580173399704 48-49 0.040705318256163955 50-51 0.02838620185699826 52-53 0.04356802041500091 54-55 0.04200438807468831 56-57 0.04430743874512705 58-59 0.04765178036670672 60-61 0.024716244823986894 62-63 0.026154379557810203 64-65 0.026785259417459104 66-67 0.032025632445187906 68-69 0.036116176051298535 70-71 0.03987432059157266 72-73 0.04362568147744194 74-75 0.025442095845303374 76-77 0.01884499193660919 78-79 0.018366744301068893 80-81 0.021700910441041325 82-83 0.019306280436137423 84-85 0.011912097134876077 86-87 0.013709765552155211 88-89 0.019282537645720528 90-91 0.01993716029578633 92-93 0.020133886273526306 94-95 0.023722439453679748 96-97 0.024461857783805885 98-99 0.030136384693443607 100 0.030668901564222518 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.4741317E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.391583266698625 #Duplication Level Percentage of deduplicated Percentage of total 1 75.16298378890478 46.895375616389714 2 14.719287353483889 18.367192850827085 3 4.984181069817042 9.329128447013792 4 2.0750901298922515 5.178726345003075 5 1.0504160306935901 3.2768559621847095 6 0.5883081010659084 2.2023284324476182 7 0.3408933087080645 1.4888211278723724 8 0.2165601753361084 1.080922576938693 9 0.16189668059210643 0.9090891204988069 >10 0.6543615239075236 6.799002635787548 >50 0.02966572289861217 1.286264042834496 >100 0.014954323238262106 1.7474513178767161 >500 9.252370904299552E-4 0.4097053197455444 >1k 4.318777721578222E-4 0.46089721099847847 >5k 0.0 0.0 >10k+ 4.467659922662051E-5 0.5682389935813026 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26785 0.18170018323328913 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19790 0.1342485206715248 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 6.783654404826923E-6 0.0 3 0.0 0.0 0.0 6.783654404826923E-6 0.0 4 0.0 6.783654404826923E-6 0.0 6.783654404826923E-6 0.0 5 0.0 6.783654404826923E-6 0.0 6.783654404826923E-6 0.0 6 0.0 6.783654404826923E-6 0.0 6.783654404826923E-6 1.3567308809653846E-5 7 0.0 6.783654404826923E-6 0.0 1.3567308809653846E-5 1.3567308809653846E-5 8 0.0 6.783654404826923E-6 0.0 1.3567308809653846E-5 1.3567308809653846E-5 9 0.0 6.783654404826923E-6 0.0 4.0701926428961535E-5 1.3567308809653846E-5 10-11 0.0 6.783654404826923E-6 0.0 4.748558083378846E-5 1.3567308809653846E-5 12-13 0.0 1.3567308809653846E-5 0.0 5.7661062441028845E-5 2.7134617619307688E-5 14-15 6.783654404826923E-6 3.391827202413461E-5 0.0 7.801202565550962E-5 4.4093753631375E-5 16-17 6.783654404826923E-6 4.748558083378846E-5 0.0 1.1193029767964422E-4 5.426923523861538E-5 18-19 1.3567308809653846E-5 5.087740803620192E-5 0.0 1.5263222410860576E-4 5.426923523861538E-5 20-21 1.3567308809653846E-5 5.426923523861538E-5 0.0 3.1883175702686535E-4 6.783654404826922E-5 22-23 1.3567308809653846E-5 5.426923523861538E-5 0.0 7.021082308995865E-4 6.783654404826922E-5 24-25 1.6959136012067306E-5 7.462019845309615E-5 0.0 0.001529714068288471 6.783654404826922E-5 26-27 2.0350963214480768E-5 9.836298886999037E-5 0.0 0.002883053122051442 6.783654404826922E-5 28-29 2.713461761930769E-5 1.0175481607240384E-4 0.0 0.00890693823353775 6.783654404826922E-5 30-31 2.713461761930769E-5 1.0175481607240384E-4 0.0 0.0356040101437341 7.122837125068268E-5 32-33 2.713461761930769E-5 1.0175481607240384E-4 0.0 0.09534765448704483 7.462019845309615E-5 34-35 3.052644482172115E-5 1.0514664327481731E-4 0.0 0.17435009368565918 9.497116166757692E-5 36-37 3.391827202413461E-5 1.221057792868846E-4 0.0 0.30968739088915864 9.497116166757692E-5 38-39 3.391827202413461E-5 1.3906491529895192E-4 0.0 0.5745246506808042 9.836298886999037E-5 40-41 3.391827202413461E-5 1.4245674250136538E-4 0.0 0.9119436207768953 1.0175481607240384E-4 42-43 3.391827202413461E-5 1.5602405131101923E-4 0.0 1.222197446808857 1.0853847047723077E-4 44-45 3.391827202413461E-5 1.5602405131101923E-4 0.0 1.5442650069868247 1.1532212488205769E-4 46-47 3.391827202413461E-5 1.6959136012067308E-4 0.0 1.9188177012949386 1.1532212488205769E-4 48-49 3.391827202413461E-5 1.7298318732308655E-4 0.0 2.3437017194596654 1.2549760648929807E-4 50-51 3.391827202413461E-5 1.7976684172791346E-4 0.0 2.813252710052976 1.2888943369171154E-4 52-53 4.748558083378846E-5 1.8994232333515384E-4 0.0 3.277030810747778 1.2888943369171154E-4 54-55 4.748558083378846E-5 1.9672597773998077E-4 0.0 3.7417891495040774 1.3228126089412498E-4 56-57 4.748558083378846E-5 1.9672597773998077E-4 0.0 4.241425647382795 1.4245674250136536E-4 58-59 4.748558083378846E-5 1.9672597773998077E-4 0.0 4.81130010296909 1.492403969061923E-4 60-61 4.748558083378846E-5 2.1707694095446153E-4 0.0 5.4032519618158945 1.5263222410860576E-4 62-63 4.748558083378846E-5 2.1707694095446153E-4 0.0 6.021856798819265 1.6280770571584614E-4 64-65 4.748558083378846E-5 2.1707694095446153E-4 0.0 6.588125742089394 1.6959136012067308E-4 66-67 4.748558083378846E-5 2.374279041689423E-4 0.0 7.174637110103527 1.6959136012067308E-4 68-69 4.748558083378846E-5 2.374279041689423E-4 0.0 7.821350019133297 1.763750145255E-4 70-71 4.748558083378846E-5 2.476033857761827E-4 0.0 8.513933999248508 1.8655049613274037E-4 72-73 4.748558083378846E-5 2.5777886738342307E-4 0.0 9.18932141544748 2.0690145934722115E-4 74-75 4.748558083378846E-5 2.5777886738342307E-4 0.0 9.830241083615526 2.2386059535928844E-4 76-77 4.748558083378846E-5 2.7812983059790383E-4 0.0 10.476638552715473 2.3403607696652882E-4 78-79 4.748558083378846E-5 2.7812983059790383E-4 0.0 11.171701958515648 2.476033857761827E-4 80-81 4.748558083378846E-5 2.815216578003173E-4 3.3918272024134614E-6 11.897898946206773 2.5777886738342307E-4 82-83 4.748558083378846E-5 2.916971394075577E-4 6.783654404826923E-6 12.631513859989578 2.7134617619307695E-4 84-85 4.748558083378846E-5 2.916971394075577E-4 6.783654404826923E-6 13.33078991517515 2.7812983059790383E-4 86-87 5.087740803620192E-5 2.916971394075577E-4 6.783654404826923E-6 14.016807317826487 2.883053122051442E-4 88 5.426923523861538E-5 2.916971394075577E-4 6.783654404826923E-6 14.541149885047584 3.052644482172115E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 14970 0.0 47.09786 2 GGTATCA 17445 0.0 46.451977 1 GTATCAA 31210 0.0 40.01867 1 ACCTGGG 17870 0.0 38.484573 3 GTACCTG 21970 0.0 32.66003 1 CAACGCA 37590 0.0 32.28872 5 ATCAACG 37895 0.0 31.929605 3 TCAACGC 39745 0.0 30.573277 4 TATCAAC 40275 0.0 30.50114 2 AACGCAG 40415 0.0 30.485775 6 GTACATG 45340 0.0 29.116108 1 CCTGGGG 17495 0.0 28.669125 4 TACATGG 45610 0.0 28.243979 2 ACATGGG 45085 0.0 27.59871 3 ACGCAGA 44845 0.0 27.013298 7 CGCAGAG 45455 0.0 26.442513 8 CATGGGG 33465 0.0 25.326132 4 GTACTAG 5990 0.0 25.24788 1 TGGGGGG 28160 0.0 24.20517 6 ATGGGGG 25650 0.0 23.421268 5 >>END_MODULE