FastQCFastQC Report
Mon 6 Jun 2016
ERR1211416_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211416_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14741317
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT250680.1700526486202013No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196030.13297997729782218No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150050.10178873434442798No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG153500.046.7199132
GGTATCA178950.044.3243751
GTATCAA309500.039.3913761
ACCTGGG178950.039.367213
GTACCTG224550.032.4128461
CAACGCA376700.031.2962065
ATCAACG376650.031.2560773
TCAACGC394600.029.8942224
CCTGGGG173100.029.8381024
AACGCAG408100.029.3591886
TATCAAC407800.029.3519942
GTACATG452350.028.1366861
TACATGG454600.027.3950632
ACATGGG448600.026.9239373
TGGGGGG251450.026.2539796
ACGCAGA455250.025.884997
ATAACGC17250.025.3371963
CGCAGAG465300.025.123948
CATGGGG328200.025.0020894
TAACGCA17950.024.873094
Produced by FastQC (version 0.11.5)