FastQCFastQC Report
Mon 6 Jun 2016
ERR1211415_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211415_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9656705
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280730.29070992641900106No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225460.23347508285693724No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135290.14009954741291156No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA171100.048.553991
GTATCAA300350.040.1324351
TACCTGG67700.035.7352332
CAACGCA363000.032.154425
ATCAACG365850.031.9811943
TATCAAC380600.031.2629892
AACGCAG390200.030.5275676
TCAACGC385400.030.4564764
GTACATG374250.029.4755731
TACATGG383550.028.2859752
ACCTGGG83650.027.98573
GTACTAG43200.027.5968441
ACGCAGA425350.027.4964587
CGCAGAG430350.027.0786848
ACATGGG386050.026.9226633
CATGGGG294900.025.5842724
ATGGGGG235200.025.0828555
TGGGGGG239150.024.8849126
GCAGAGT464300.024.2983259
GCATCGT41300.023.6747888
Produced by FastQC (version 0.11.5)