##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211415_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9656705 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.88582637659533 31.0 31.0 34.0 27.0 34.0 2 30.94071456050485 31.0 31.0 34.0 27.0 34.0 3 30.992572414710814 31.0 31.0 34.0 27.0 34.0 4 34.60398852403589 37.0 35.0 37.0 30.0 37.0 5 34.65447386039027 37.0 35.0 37.0 30.0 37.0 6 34.71782414394972 37.0 35.0 37.0 31.0 37.0 7 34.63097505826263 37.0 35.0 37.0 30.0 37.0 8 34.629257288070825 37.0 35.0 37.0 30.0 37.0 9 36.12524769059426 39.0 35.0 39.0 30.0 39.0 10-11 36.03680598092207 39.0 35.0 39.0 30.0 39.0 12-13 35.93012766777073 39.0 35.0 39.0 30.0 39.0 14-15 37.02774434965136 40.0 36.0 41.0 30.0 41.0 16-17 37.010100028943626 40.0 36.0 41.0 30.0 41.0 18-19 37.010445384838825 40.0 36.0 41.0 30.0 41.0 20-21 36.99942728912191 40.0 36.0 41.0 30.0 41.0 22-23 36.87645739411114 39.5 36.0 41.0 30.0 41.0 24-25 36.77444117843508 39.0 36.0 41.0 30.0 41.0 26-27 36.62415321789368 39.0 36.0 41.0 29.0 41.0 28-29 36.49461855777928 39.0 35.0 41.0 29.0 41.0 30-31 36.333658219858634 39.0 35.0 40.5 29.0 41.0 32-33 36.13731598925306 39.0 35.0 40.0 28.0 41.0 34-35 36.02220327741191 39.0 35.0 40.0 27.5 41.0 36-37 35.89599568382798 38.0 35.0 40.0 27.0 41.0 38-39 35.74625164587714 38.0 34.5 40.0 27.0 41.0 40-41 35.58201493159416 38.0 34.0 40.0 26.5 41.0 42-43 35.39264526564703 38.0 34.0 40.0 26.0 41.0 44-45 35.25238039269088 38.0 34.0 40.0 25.0 41.0 46-47 35.02511218888844 38.0 33.0 40.0 24.0 41.0 48-49 34.77885940390641 38.0 33.0 40.0 24.0 41.0 50-51 34.14442840492694 37.5 32.5 39.5 23.5 40.5 52-53 34.47999938902555 37.5 33.0 39.5 24.0 40.5 54-55 34.89621014621447 38.0 33.0 40.0 24.0 41.0 56-57 34.82311777153801 38.0 33.0 40.0 24.0 41.0 58-59 34.61983481943375 38.0 33.0 40.0 24.0 41.0 60-61 34.39982437073515 37.0 33.0 40.0 23.0 41.0 62-63 34.117530565550055 37.0 32.5 40.0 23.0 41.0 64-65 33.914339932720324 36.0 32.0 40.0 23.0 41.0 66-67 33.71915767334717 36.0 32.0 39.0 22.5 41.0 68-69 33.38473501054449 35.5 32.0 39.0 22.0 41.0 70-71 33.02310337739425 35.0 31.5 39.0 21.5 40.5 72-73 32.65433302560242 35.0 31.0 38.0 20.5 40.0 74-75 32.297214836737794 35.0 31.0 37.0 20.0 39.5 76-77 31.936696989293967 35.0 30.5 37.0 20.0 39.0 78-79 31.570413873055045 35.0 30.0 36.0 19.5 39.0 80-81 31.216593962433357 34.0 30.0 36.0 18.5 38.0 82-83 30.886595738401454 34.0 29.5 35.0 18.0 37.0 84-85 30.585621596600497 34.0 29.0 35.0 18.0 37.0 86-87 30.28642083402154 34.0 29.0 35.0 17.0 36.0 88-89 30.02170916477204 34.0 29.0 35.0 16.0 36.0 90-91 29.75255576306825 34.0 29.0 35.0 15.0 36.0 92-93 29.497865058526692 34.0 27.5 35.0 12.5 35.0 94-95 29.260161307609582 34.0 27.0 35.0 11.0 35.0 96-97 29.010618994781346 34.0 26.5 35.0 10.0 35.0 98-99 28.73609181392618 33.0 25.5 35.0 10.0 35.0 100 27.501218479802375 32.0 24.0 35.0 8.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.2579740075630177 1101 2 -0.21557109963115906 1101 3 -0.23917369424601986 1101 4 -0.10864149199350948 1101 5 -0.12564780622651028 1101 6 -0.15642955501844114 1101 7 -0.16488605655894162 1101 8 -0.25106085426845937 1101 9 -0.3921413474464117 1101 10-11 -0.3128829682623646 1101 12-13 -0.40477182529396316 1101 14-15 -0.5551528789765925 1101 16-17 -0.43904219191392 1101 18-19 -0.5353542230513213 1101 20-21 -0.5306859099041645 1101 22-23 -0.5622418630971637 1101 24-25 -0.5831532160822945 1101 26-27 -0.6712520458869236 1101 28-29 -0.5707133821286732 1101 30-31 -0.633508472646966 1101 32-33 -0.6666353086442669 1101 34-35 -0.6601254100683107 1101 36-37 -0.6991282279910465 1101 38-39 -0.8109697523119621 1101 40-41 -0.9522635941656361 1101 42-43 -1.0091206566565631 1101 44-45 -1.074171499531836 1101 46-47 -1.0315892137690525 1101 48-49 -1.0645231911056285 1101 50-51 -0.9140144236611647 1101 52-53 -0.771262586524827 1101 54-55 -0.7501923878098893 1101 56-57 -0.829767995606602 1101 58-59 -0.7720664449533174 1101 60-61 -0.806052457475694 1101 62-63 -0.8748560667278227 1101 64-65 -0.8113224062200217 1101 66-67 -0.8852238448950871 1101 68-69 -0.8962322212598579 1101 70-71 -0.8358918222191178 1101 72-73 -0.9772864869554958 1101 74-75 -1.0737854127976902 1101 76-77 -1.0269844363731586 1101 78-79 -1.0742559639157676 1101 80-81 -1.1527356289205812 1101 82-83 -1.1429260030541357 1101 84-85 -1.1491203428002628 1101 86-87 -1.2675038743922364 1101 88-89 -1.3210371481454857 1101 90-91 -1.4917276743408756 1101 92-93 -1.5521086978943082 1101 94-95 -1.5681190918642685 1101 96-97 -1.6765618183775892 1101 98-99 -1.7707729379533461 1101 100 -1.7839670601899584 1102 1 -0.38065538847126845 1102 2 -0.41706807981223193 1102 3 -0.4171873383864977 1102 4 -0.3245387824305155 1102 5 -0.30686256031776793 1102 6 -0.23240305443996334 1102 7 -0.3896233631716015 1102 8 -0.294183758456974 1102 9 -0.4938283669973842 1102 10-11 -0.3895803874534707 1102 12-13 -0.4816209205428379 1102 14-15 -0.6783153457150206 1102 16-17 -0.6134129756240085 1102 18-19 -0.6895001514341033 1102 20-21 -0.7720702677211193 1102 22-23 -0.6958101476847176 1102 24-25 -0.7522639104140367 1102 26-27 -0.7608512697552214 1102 28-29 -0.8251190145878198 1102 30-31 -0.7724870294790662 1102 32-33 -0.8865222898524223 1102 34-35 -0.8694555854088222 1102 36-37 -0.7961574267374232 1102 38-39 -0.8930359271662169 1102 40-41 -1.0962637981765937 1102 42-43 -1.0015585343242392 1102 44-45 -1.0236916697392786 1102 46-47 -1.0735927073688813 1102 48-49 -1.1717113772783918 1102 50-51 -0.9293923498628303 1102 52-53 -0.787451110803012 1102 54-55 -0.9227429270745588 1102 56-57 -0.8731997269464244 1102 58-59 -0.8745357212191678 1102 60-61 -0.9628076356591251 1102 62-63 -0.9146806717610829 1102 64-65 -1.0744685965847296 1102 66-67 -1.074681572758756 1102 68-69 -1.030990550253172 1102 70-71 -0.9678945686375968 1102 72-73 -1.003021877949756 1102 74-75 -1.128351566278809 1102 76-77 -1.1707352041956902 1102 78-79 -1.190374914250107 1102 80-81 -1.2561243891347473 1102 82-83 -1.319606475984795 1102 84-85 -1.3714516786141289 1102 86-87 -1.367596677712438 1102 88-89 -1.4502549751664446 1102 90-91 -1.5069153064517344 1102 92-93 -1.5183305818289377 1102 94-95 -1.63610953532109 1102 96-97 -1.7366902861393463 1102 98-99 -1.80925694973633 1102 100 -1.7180812702558974 1103 1 -0.3981720135219149 1103 2 -0.4477747132167984 1103 3 -0.5207468975782419 1103 4 -0.3604215061714555 1103 5 -0.3279362968412727 1103 6 -0.32788545807687086 1103 7 -0.3867060278217309 1103 8 -0.335603135924309 1103 9 -0.6062038320999648 1103 10-11 -0.5006723263664696 1103 12-13 -0.5676701467613015 1103 14-15 -0.6996856017838482 1103 16-17 -0.7114952310731368 1103 18-19 -0.7176580615544452 1103 20-21 -0.689592119371504 1103 22-23 -0.6816679190913888 1103 24-25 -0.7192766653331546 1103 26-27 -0.8250887002148062 1103 28-29 -0.7421438209228626 1103 30-31 -0.7945247928860155 1103 32-33 -0.853441815478746 1103 34-35 -0.8327680085036491 1103 36-37 -0.9120679162495264 1103 38-39 -0.9040129528767622 1103 40-41 -1.0041140069958132 1103 42-43 -0.9025024467177829 1103 44-45 -0.9092712506955607 1103 46-47 -1.0139321080693904 1103 48-49 -0.9688824333769404 1103 50-51 -0.9397831469005737 1103 52-53 -0.722056514041661 1103 54-55 -0.7747432461419166 1103 56-57 -0.7723271151641384 1103 58-59 -0.7569257660872992 1103 60-61 -0.8717647173562071 1103 62-63 -0.792116519208875 1103 64-65 -0.7518364550409231 1103 66-67 -0.8231613521530576 1103 68-69 -0.8496385829639124 1103 70-71 -0.9154654236200486 1103 72-73 -1.038867050803347 1103 74-75 -0.9744795053772606 1103 76-77 -1.1354042682830148 1103 78-79 -1.1183153229500498 1103 80-81 -1.0360197477126043 1103 82-83 -1.0695942517887218 1103 84-85 -1.0802577504543898 1103 86-87 -1.1676753934720914 1103 88-89 -1.210052430748263 1103 90-91 -1.2707035804960363 1103 92-93 -1.3313518271649514 1103 94-95 -1.2951789385182693 1103 96-97 -1.4732455854363309 1103 98-99 -1.494889329428787 1103 100 -1.5187082606473048 1104 1 -0.40926486281585994 1104 2 -0.3666700446655504 1104 3 -0.3836837780918678 1104 4 -0.29513351285316247 1104 5 -0.30601705770457954 1104 6 -0.32508068296399273 1104 7 -0.3521610913820865 1104 8 -0.46355468250791176 1104 9 -0.44448260706219145 1104 10-11 -0.5007977882812114 1104 12-13 -0.5379342883309661 1104 14-15 -0.7499262931494783 1104 16-17 -0.7276115001091199 1104 18-19 -0.6710826968965691 1104 20-21 -0.6597741381493876 1104 22-23 -0.7189452003077363 1104 24-25 -0.717271767043016 1104 26-27 -0.6679011628296223 1104 28-29 -0.8211345372119467 1104 30-31 -0.8293029021150033 1104 32-33 -0.9113446007027832 1104 34-35 -0.8280386578649441 1104 36-37 -0.9017748883928505 1104 38-39 -0.9607606050870885 1104 40-41 -0.9207946261831026 1104 42-43 -0.9881100916836871 1104 44-45 -0.9195995313979424 1104 46-47 -0.8493792459703187 1104 48-49 -0.9197971519523307 1104 50-51 -0.8951913986695885 1104 52-53 -0.7566834071167037 1104 54-55 -0.6692342343736115 1104 56-57 -0.6068891103256604 1104 58-59 -0.6401662364649425 1104 60-61 -0.7517938694934188 1104 62-63 -0.6739822670676787 1104 64-65 -0.6835048229643306 1104 66-67 -0.6022887459126665 1104 68-69 -0.8204864180570226 1104 70-71 -0.7659026779855296 1104 72-73 -0.8162796137766151 1104 74-75 -0.8537517598092066 1104 76-77 -0.8940576095770858 1104 78-79 -0.7783408528781379 1104 80-81 -0.8307603997545527 1104 82-83 -0.9140588488484163 1104 84-85 -0.9855485850468213 1104 86-87 -1.0543715842816503 1104 88-89 -1.0868131760814848 1104 90-91 -1.0006883273401748 1104 92-93 -1.0525687925069427 1104 94-95 -1.206030431821059 1104 96-97 -1.290635803145502 1104 98-99 -1.380206699195412 1104 100 -1.288599090365814 1105 1 -0.5085248906400395 1105 2 -0.46711552742727847 1105 3 -0.4243636361826546 1105 4 -0.3049039338886814 1105 5 -0.35497440912573097 1105 6 -0.2895569957674127 1105 7 -0.3901361631327518 1105 8 -0.35665614136089374 1105 9 -0.46636782368728547 1105 10-11 -0.4740645711346829 1105 12-13 -0.5098939697007268 1105 14-15 -0.6542452825623073 1105 16-17 -0.6919459094918423 1105 18-19 -0.6068670488703347 1105 20-21 -0.7288685339738237 1105 22-23 -0.5977415281352094 1105 24-25 -0.7332109131279552 1105 26-27 -0.7781658989885045 1105 28-29 -0.7448522020278858 1105 30-31 -0.8188551722749011 1105 32-33 -0.862376140248422 1105 34-35 -0.8854622640445555 1105 36-37 -0.8110929822385913 1105 38-39 -0.8931323252371683 1105 40-41 -0.8343965290815234 1105 42-43 -0.7990944720671038 1105 44-45 -0.8342442487209567 1105 46-47 -0.8857379007390591 1105 48-49 -0.9004423467005367 1105 50-51 -0.9096232174257608 1105 52-53 -0.7381608185034736 1105 54-55 -0.6485883462322093 1105 56-57 -0.7039857723928762 1105 58-59 -0.6265006514285005 1105 60-61 -0.6361066483775417 1105 62-63 -0.7489713475724713 1105 64-65 -0.7178009488285184 1105 66-67 -0.658982472815353 1105 68-69 -0.6475216929755874 1105 70-71 -0.6711449807417509 1105 72-73 -0.7171963827938086 1105 74-75 -0.7149113127985558 1105 76-77 -0.8181003847252022 1105 78-79 -0.8483741857692166 1105 80-81 -0.8658129797037901 1105 82-83 -0.9180876239822098 1105 84-85 -0.9005032759861429 1105 86-87 -0.911144872395429 1105 88-89 -0.9500607008204085 1105 90-91 -1.0402954003089384 1105 92-93 -0.9922935080314552 1105 94-95 -1.107123115801393 1105 96-97 -1.126677489818956 1105 98-99 -1.1730701404306245 1105 100 -1.0483298926928342 1106 1 -0.47857427581760703 1106 2 -0.5214469105806145 1106 3 -0.6145095414595048 1106 4 -0.3742238465218577 1106 5 -0.40443891910272356 1106 6 -0.5006332353419793 1106 7 -0.5185214478017599 1106 8 -0.49742554605503386 1106 9 -0.731506610207397 1106 10-11 -0.7721890757922907 1106 12-13 -0.6882837218262594 1106 14-15 -0.8321341987550994 1106 16-17 -0.7989767958184402 1106 18-19 -0.8618902454621846 1106 20-21 -0.887951192361875 1106 22-23 -0.8448955905519355 1106 24-25 -0.8255215490025378 1106 26-27 -0.8537320182590236 1106 28-29 -0.8726589898923365 1106 30-31 -0.9544521437901352 1106 32-33 -1.0083236000048146 1106 34-35 -0.936550546719964 1106 36-37 -0.9770979256895842 1106 38-39 -1.0105434619802267 1106 40-41 -1.0593699891594 1106 42-43 -1.0346712861881286 1106 44-45 -1.0103607566313926 1106 46-47 -0.9823203666182891 1106 48-49 -0.9806789613781746 1106 50-51 -0.9528537474226155 1106 52-53 -0.9258179448215813 1106 54-55 -0.8631822257616832 1106 56-57 -0.9793498193896184 1106 58-59 -0.8147201726759263 1106 60-61 -0.767429974357853 1106 62-63 -0.8928816884747093 1106 64-65 -0.8968327413175601 1106 66-67 -0.8787499624881789 1106 68-69 -0.8682859525821911 1106 70-71 -0.8900619883962975 1106 72-73 -0.7844621231093214 1106 74-75 -0.8932376215323075 1106 76-77 -0.9720003678395166 1106 78-79 -0.9705808390891022 1106 80-81 -1.00387799557015 1106 82-83 -0.9261429376548769 1106 84-85 -1.0242411037009376 1106 86-87 -1.0470518738739187 1106 88-89 -1.0066426215397328 1106 90-91 -1.0116529752032513 1106 92-93 -1.0628500033639021 1106 94-95 -1.0447588313833762 1106 96-97 -1.1319055220468464 1106 98-99 -1.1705846795099397 1106 100 -1.234897262625804 1107 1 -0.49323152895775024 1107 2 -0.5071032558074151 1107 3 -0.5092033848846462 1107 4 -0.38400651585175183 1107 5 -0.3972702948004567 1107 6 -0.43148746973255925 1107 7 -0.48232164735087224 1107 8 -0.43687440330698735 1107 9 -0.5686894086744729 1107 10-11 -0.5824633523179514 1107 12-13 -0.6370368887315436 1107 14-15 -0.7510403450898124 1107 16-17 -0.8356254170656783 1107 18-19 -0.8477184206647976 1107 20-21 -0.7514231566034439 1107 22-23 -0.7757055591679105 1107 24-25 -0.7828295524502948 1107 26-27 -0.7692411508237953 1107 28-29 -0.7663662407964011 1107 30-31 -0.8320446834797224 1107 32-33 -0.8291620558925459 1107 34-35 -0.8482012147198574 1107 36-37 -0.7847740753619519 1107 38-39 -0.8640016789728193 1107 40-41 -0.824384647555199 1107 42-43 -0.845062502642854 1107 44-45 -0.8361227360543708 1107 46-47 -0.8610398449777179 1107 48-49 -0.924627404532707 1107 50-51 -0.9247030122430431 1107 52-53 -0.786532205399169 1107 54-55 -0.7010831776174555 1107 56-57 -0.705519519235331 1107 58-59 -0.7662086702785231 1107 60-61 -0.7299973024916184 1107 62-63 -0.6651136313116552 1107 64-65 -0.6489850711590535 1107 66-67 -0.7192937567408038 1107 68-69 -0.6098509581941514 1107 70-71 -0.6134698941116952 1107 72-73 -0.6772222578353038 1107 74-75 -0.6658085503704818 1107 76-77 -0.6424298482060031 1107 78-79 -0.6107278374423046 1107 80-81 -0.6614613329868568 1107 82-83 -0.6524161208286685 1107 84-85 -0.7023404819512677 1107 86-87 -0.7783820951059077 1107 88-89 -0.7332837461109136 1107 90-91 -0.8316804611920681 1107 92-93 -0.8448086192954811 1107 94-95 -0.8200994253086336 1107 96-97 -0.8556474723828984 1107 98-99 -0.8486998604533902 1107 100 -0.9443507217435503 1108 1 -0.5340003487851348 1108 2 -0.5627872848976665 1108 3 -0.5759303411680676 1108 4 -0.5224059956147471 1108 5 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72-73 1.5271598948581726 2311 74-75 1.4933711405044434 2311 76-77 1.4351578367109532 2311 78-79 1.5074520795000552 2311 80-81 1.5550590332712417 2311 82-83 1.5347775057829836 2311 84-85 1.4996695211819997 2311 86-87 1.5801858245391927 2311 88-89 1.615797821156665 2311 90-91 1.6805269675564922 2311 92-93 1.7724586446243293 2311 94-95 1.8716390445283508 2311 96-97 1.8480291549360182 2311 98-99 1.9133611588202513 2311 100 1.912007704023594 2312 1 0.5855420969669076 2312 2 0.6743648371610718 2312 3 0.7161865654326149 2312 4 0.6036441957226799 2312 5 0.6671905364581008 2312 6 0.5185431867826225 2312 7 0.6755608241816162 2312 8 0.7080725406719068 2312 9 0.8346256886850938 2312 10-11 0.8773927848760295 2312 12-13 0.9778032997719492 2312 14-15 1.162345176482745 2312 16-17 1.1469792698688082 2312 18-19 1.119270998568041 2312 20-21 1.1175114529701418 2312 22-23 1.1584409610152662 2312 24-25 1.2138899397553828 2312 26-27 1.2170765883685633 2312 28-29 1.2838892119548717 2312 30-31 1.1334153552462567 2312 32-33 1.2878427812031816 2312 34-35 1.3495877278472435 2312 36-37 1.3641330494368162 2312 38-39 1.344045581032013 2312 40-41 1.3880272630007298 2312 42-43 1.4536615762428582 2312 44-45 1.430537080358441 2312 46-47 1.4184353416260578 2312 48-49 1.4975685852167473 2312 50-51 1.4234844899383958 2312 52-53 1.2854788840386462 2312 54-55 1.273563853660967 2312 56-57 1.2585148894709377 2312 58-59 1.2980429349837834 2312 60-61 1.2760408062334108 2312 62-63 1.2708935694283312 2312 64-65 1.2327057930614203 2312 66-67 1.241108014481064 2312 68-69 1.2108522435558484 2312 70-71 1.294335104768102 2312 72-73 1.2863241996605623 2312 74-75 1.3202434875234914 2312 76-77 1.3037602338032634 2312 78-79 1.2900845261887213 2312 80-81 1.1422163908213747 2312 82-83 1.3208487078577633 2312 84-85 1.375099630126087 2312 86-87 1.3715678725489688 2312 88-89 1.4213300388193666 2312 90-91 1.496517050525096 2312 92-93 1.5189127199678651 2312 94-95 1.5644921304137256 2312 96-97 1.6333742818498145 2312 98-99 1.5358674730856805 2312 100 1.6092021056833943 2313 1 0.5148408979971606 2313 2 0.5634842209116755 2313 3 0.6393107852637563 2313 4 0.5626374946705965 2313 5 0.5552067265044158 2313 6 0.5859072701425703 2313 7 0.6207008070662212 2313 8 0.6480635280304199 2313 9 0.8098029531595898 2313 10-11 0.7413685194315889 2313 12-13 0.88139311724143 2313 14-15 1.0880772863447916 2313 16-17 1.0281683839894882 2313 18-19 1.0116320489704407 2313 20-21 1.0482447537140445 2313 22-23 1.0886796220656052 2313 24-25 1.1556942491717592 2313 26-27 1.1530002607211358 2313 28-29 1.1344016256716998 2313 30-31 1.1445603665960533 2313 32-33 1.1894478996461189 2313 34-35 1.202048244389644 2313 36-37 1.2400542062187156 2313 38-39 1.2666120191564119 2313 40-41 1.2430605303821025 2313 42-43 1.2922221970357484 2313 44-45 1.2496805731480336 2313 46-47 1.2856637967742657 2313 48-49 1.3141372308584849 2313 50-51 1.2914394201892847 2313 52-53 1.0940602062535092 2313 54-55 1.033143702809987 2313 56-57 1.127964807610816 2313 58-59 1.0558160706515451 2313 60-61 1.0480399623575707 2313 62-63 1.0886615788031406 2313 64-65 1.0470518383523952 2313 66-67 1.062678638978916 2313 68-69 1.1140850417803208 2313 70-71 1.1457845611253248 2313 72-73 1.1823903273528344 2313 74-75 1.1162782226184405 2313 76-77 1.1360505047891003 2313 78-79 1.2065726572148634 2313 80-81 1.2006600108167689 2313 82-83 1.2164780673625515 2313 84-85 1.3869648796879446 2313 86-87 1.4142413101446785 2313 88-89 1.4659843247749293 2313 90-91 1.4997887271023949 2313 92-93 1.5970205648017561 2313 94-95 1.6088245883522525 2313 96-97 1.6686852744773475 2313 98-99 1.637290160619095 2313 100 1.7681598417173667 2314 1 0.4776124189531856 2314 2 0.49508789512058016 2314 3 0.5342997290441325 2314 4 0.4868039059273386 2314 5 0.4067639767120568 2314 6 0.5077476905963749 2314 7 0.5513136927712878 2314 8 0.570792601876505 2314 9 0.6688629638146537 2314 10-11 0.6404791878321561 2314 12-13 0.4082983310094406 2314 14-15 0.8170159166780238 2314 16-17 0.8410207198215076 2314 18-19 0.8703507338372987 2314 20-21 0.8711948942327936 2314 22-23 0.7230353599001589 2314 24-25 0.7764825827388577 2314 26-27 0.9202631003369746 2314 28-29 0.9832317832639461 2314 30-31 0.876719643979321 2314 32-33 0.9646200921138117 2314 34-35 1.0082227721987351 2314 36-37 1.049244504797251 2314 38-39 1.0948546862940702 2314 40-41 1.1238906299660059 2314 42-43 1.1852090300045788 2314 44-45 1.2906980801277896 2314 46-47 1.2751651860888344 2314 48-49 1.3392943440394234 2314 50-51 1.2315164702940748 2314 52-53 1.0721666547625759 2314 54-55 1.0899206517320366 2314 56-57 1.1179560067907417 2314 58-59 1.108609930596863 2314 60-61 1.1454149399190143 2314 62-63 1.1584189869897656 2314 64-65 1.128420658081474 2314 66-67 1.172207471152376 2314 68-69 1.2199581642287498 2314 70-71 1.2133420745350705 2314 72-73 1.1892654936578921 2314 74-75 1.2297021355678126 2314 76-77 1.235050026580911 2314 78-79 1.2999730306039794 2314 80-81 1.3438409841912176 2314 82-83 1.2633598440228724 2314 84-85 1.2311833440653324 2314 86-87 1.430027512409719 2314 88-89 1.516955654505395 2314 90-91 1.5312666948936169 2314 92-93 1.6216313171845727 2314 94-95 1.6587859016845385 2314 96-97 1.7028105156737858 2314 98-99 1.7831841141281402 2314 100 1.6275342663235293 2315 1 0.2906226761858619 2315 2 0.31207707444133703 2315 3 0.37391941917060123 2315 4 0.3263541736621107 2315 5 0.278493094021421 2315 6 -0.40477733596353005 2315 7 0.15737476010382778 2315 8 0.2249227522589905 2315 9 0.4948052538933396 2315 10-11 0.5576392842320459 2315 12-13 0.6117640666432891 2315 14-15 0.6560482126964686 2315 16-17 0.5552696529776782 2315 18-19 0.6461530093546912 2315 20-21 0.6077118874967837 2315 22-23 0.5952768654124014 2315 24-25 0.7273809212253681 2315 26-27 0.7497948359022857 2315 28-29 0.8472924862032443 2315 30-31 0.826376383159058 2315 32-33 0.865832103045264 2315 34-35 0.8878518816911836 2315 36-37 0.9502714848862723 2315 38-39 0.9669340576150844 2315 40-41 0.9374598860624062 2315 42-43 1.0028353561474077 2315 44-45 1.043379047637309 2315 46-47 1.0237820457773452 2315 48-49 1.0738995040805364 2315 50-51 1.031476814610464 2315 52-53 0.913053420302866 2315 54-55 0.7036874771615587 2315 56-57 0.6927619138847234 2315 58-59 0.7081177576849171 2315 60-61 0.5983512183582604 2315 62-63 0.7646554679343396 2315 64-65 0.8438871262383074 2315 66-67 0.8589775674168223 2315 68-69 0.9230401766153093 2315 70-71 0.9352697080395274 2315 72-73 0.9528641406897762 2315 74-75 0.996159955477971 2315 76-77 0.9819691266510127 2315 78-79 0.9199769484046385 2315 80-81 0.9541193875135789 2315 82-83 1.0764443004173145 2315 84-85 1.0638949860815288 2315 86-87 1.1227694310807301 2315 88-89 1.2562266680158416 2315 90-91 1.3342145055173162 2315 92-93 1.4358274022796955 2315 94-95 1.5635748323499925 2315 96-97 1.5535425440105186 2315 98-99 1.5945225442963675 2315 100 1.6879852044578207 2316 1 -0.11457321174949087 2316 2 -0.17250629665322847 2316 3 -0.10415268664584332 2316 4 -0.16404739049852424 2316 5 -0.11344422854594427 2316 6 -0.031235841029172207 2316 7 -0.04465470565529728 2316 8 -0.030142709459354933 2316 9 0.05795439479455666 2316 10-11 -0.10247102048701606 2316 12-13 -0.01403453827968093 2316 14-15 0.15338375114129832 2316 16-17 0.18687958528786908 2316 18-19 0.1320886301009523 2316 20-21 0.1319566953452238 2316 22-23 -0.02343382947957906 2316 24-25 0.06572742871235704 2316 26-27 0.11896925319766893 2316 28-29 0.2698886783387593 2316 30-31 0.2929901768715695 2316 32-33 0.3956219944822621 2316 34-35 0.3020548898413935 2316 36-37 0.3985265256315529 2316 38-39 0.45231825594445496 2316 40-41 0.38921779615554186 2316 42-43 0.4447541702900182 2316 44-45 0.27482932039266217 2316 46-47 0.24358010520406737 2316 48-49 0.41737857626281283 2316 50-51 0.0956171427263115 2316 52-53 -0.0383348271870787 2316 54-55 -0.046278013768443316 2316 56-57 0.034221970593783624 2316 58-59 0.12947595951882107 2316 60-61 0.23604247991012528 2316 62-63 0.1748137239998684 2316 64-65 0.22815924358909712 2316 66-67 0.26494508538429784 2316 68-69 0.22799816516509708 2316 70-71 0.20857951883442638 2316 72-73 0.31216585634342664 2316 74-75 0.36132279275806667 2316 76-77 0.4083853102268371 2316 78-79 0.457406100019206 2316 80-81 0.5794890681843512 2316 82-83 0.5642072035211925 2316 84-85 0.6378306866142047 2316 86-87 0.7971441592540494 2316 88-89 0.8019397309165903 2316 90-91 0.7727800498406232 2316 92-93 0.8254029313236124 2316 94-95 0.9115181857296619 2316 96-97 1.013232119929821 2316 98-99 1.0758205073867657 2316 100 0.9374290483360426 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 59.0 1 549.0 2 249.0 3 184.0 4 176.0 5 148.0 6 175.0 7 826.0 8 2985.0 9 5548.0 10 9202.0 11 15336.0 12 23191.0 13 29630.0 14 34458.0 15 40467.0 16 47612.0 17 56299.0 18 66791.0 19 78436.0 20 91646.0 21 107198.0 22 115879.0 23 119187.0 24 122119.0 25 129800.0 26 143094.0 27 162575.0 28 188897.0 29 220752.0 30 260681.0 31 314021.0 32 386546.0 33 491851.0 34 662789.0 35 870525.0 36 1226263.0 37 1701612.0 38 1538080.0 39 390523.0 40 346.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.83223412794539 19.524870520849618 13.155530037855176 23.48736531334982 2 15.698837235712174 20.449051422583516 41.10945325074015 22.74265809096416 3 18.28962620453509 27.169292174148485 30.304136402991737 24.23694521832469 4 12.473464630780438 17.311869087448457 38.82223822155877 31.392428060212335 5 12.812620577320978 38.23558445526889 34.51697858158342 14.43481638582671 6 29.283567305570262 37.59067827254614 19.287950075791876 13.837804346091719 7 26.447005809700297 33.15367210610236 23.293066367028654 17.106255717168686 8 25.772430866006367 35.35655712354263 20.799665461708535 18.07134654874247 9 27.510179311065013 15.515691155615336 20.834024420401118 36.14010511291853 10-11 24.754784356569328 26.11860576462603 28.950392870801455 20.17621700800318 12-13 25.2375428564443 24.22435715074549 28.455860581851017 22.08223941095919 14-15 23.085849757551266 25.582690293034343 26.368782362080562 24.962677587333822 16-17 22.01931538154321 28.023409055882116 27.32330829871187 22.63396726386281 18-19 21.650909854344278 27.686969483811087 29.143195267865636 21.518925393979 20-21 22.795637992103345 27.060583253116743 28.551102193303795 21.592676561476118 22-23 22.73770356044363 26.800991082874724 28.38877769190345 22.072527664778196 24-25 22.214711646636097 26.65923458039329 28.825232329948168 22.300821443022446 26-27 22.1265122011849 26.881856693043876 28.521025810995564 22.470605294775655 28-29 21.818381340272268 27.743255256481973 28.479457402358538 21.958906000887218 30-31 22.948293087315903 26.914850843611738 28.579117380632734 21.557738688439628 32-33 22.813648277107543 26.981393265894116 28.207301150107256 21.99765730689109 34-35 22.712544325276944 27.191259180721172 28.53627510022065 21.559921393781234 36-37 22.438232452043913 27.07912255895067 28.48948359907154 21.993161389933874 38-39 22.467525977017328 27.12007082746061 28.562173029580407 21.850230165941657 40-41 22.397437587920294 26.499944429143323 28.970952030801485 22.1316659521349 42-43 22.197714740963388 26.893945730782203 28.271748316442906 22.636591211811503 44-45 21.68190235165713 27.282414491540642 28.75360975688843 22.282073399913795 46-47 21.690868942619232 26.731517908813956 28.414711038645525 23.162902109921284 48-49 22.11345994555233 26.79711267908036 28.40018877513149 22.68923860023582 50-51 22.021070908298295 26.95974016375617 28.31476394604827 22.70442498189726 52-53 21.615095667265265 27.289480320057418 28.072042963297246 23.023381049380067 54-55 21.48622378125879 26.95022162227401 28.483126715520086 23.080427880947116 56-57 22.01350356501493 26.785138930012742 28.015732644704215 23.185624860268113 58-59 21.61728997374766 26.954827715569614 28.454533841423586 22.973348469259147 60-61 22.041019174439576 27.086851820259916 28.090470598629864 22.781658406670644 62-63 22.022335461999926 26.777663792214078 28.04981415427246 23.150186591513535 64-65 21.56003417184169 26.522005179639113 28.155427498906562 23.76253314961264 66-67 21.766043083342353 26.31469661883139 27.991084168987474 23.928176128838782 68-69 21.310750856645317 26.5273278337796 28.270194129775 23.891727179800082 70-71 21.49959317832464 26.18385936297962 28.389931997483032 23.92661546121271 72-73 21.54604521089496 26.521767350148046 28.10219944152399 23.829987997433 74-75 21.66118161674015 26.57534267859127 27.98766038559612 23.775815319072464 76-77 21.48308254731711 26.222416757648 28.077524484689707 24.21697621034518 78-79 21.260910184081357 26.161251666240286 28.415624284694825 24.162213864983524 80-81 21.381229409569485 26.313647068303858 28.17906096605921 24.126062556067442 82-83 21.826233149950884 25.932907627530483 28.520446887518297 23.720412335000336 84-85 21.92337764606805 26.206715880714114 27.892456758019573 23.977449715198258 86-87 21.330850237868955 26.452040441735246 28.202523318117183 24.014586002278616 88-89 21.5365909007814 25.615080591945645 28.32842027026145 24.519908237011506 90-91 22.125181121301853 25.38170056893542 28.39216071738096 24.100957592381768 92-93 22.13110021021238 25.536047790729537 28.19639888492303 24.136453114135055 94-95 21.680492074768292 25.363709024908005 28.333312531044825 24.622486369278874 96-97 21.75889742282903 25.142924263023424 28.3615123229984 24.73666599114915 98-99 21.66893019369652 25.283434638727726 28.15908594873342 24.888549218842336 100 21.915886195021265 25.141535262153326 27.990233460931048 24.952345081894364 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1001.0 1 758.5 2 973.5 3 1461.0 4 1196.0 5 1014.0 6 1358.5 7 2425.5 8 4043.0 9 5077.0 10 5020.0 11 4971.5 12 5521.5 13 6245.5 14 7115.5 15 8145.0 16 9141.0 17 10104.5 18 11210.0 19 12150.0 20 13353.5 21 14942.0 22 17129.5 23 20537.5 24 24800.0 25 30215.5 26 37695.0 27 46885.5 28 58543.0 29 75223.5 30 96171.0 31 116148.0 32 140749.0 33 167357.5 34 191414.0 35 214976.5 36 240362.0 37 274440.5 38 293256.0 39 293348.5 40 293428.0 41 295174.5 42 301535.0 43 314572.0 44 331369.5 45 354999.0 46 378869.0 47 400041.0 48 421415.5 49 431629.0 50 431759.5 51 425247.5 52 404161.5 53 377782.5 54 350585.5 55 309923.5 56 271558.0 57 234685.5 58 193799.5 59 159757.0 60 126638.5 61 96917.0 62 75616.0 63 58726.0 64 43082.0 65 29398.0 66 20509.5 67 15189.0 68 11310.5 69 8232.0 70 6160.5 71 4577.5 72 3347.0 73 2469.5 74 1808.5 75 1270.5 76 872.0 77 585.5 78 378.0 79 272.5 80 213.5 81 165.0 82 138.0 83 114.0 84 88.5 85 69.5 86 49.5 87 41.0 88 36.5 89 32.0 90 28.5 91 21.5 92 18.0 93 17.0 94 13.0 95 9.5 96 6.5 97 4.5 98 3.5 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22329562723516974 2 0.14936771911330005 3 0.023341294986229773 4 0.022140057089866577 5 0.0272349626503036 6 0.05462525778720588 7 0.061604864185040346 8 0.05378646235957296 9 0.04181550539236727 10-11 0.04381929446949037 12-13 0.04277856680927915 14-15 0.02054013247790007 16-17 0.01873309788380198 18-19 0.01843796615926447 20-21 0.02189670286086196 22-23 0.025024063591048914 24-25 0.0224766108108304 26-27 0.019727225798033595 28-29 0.018805586377548037 30-31 0.017304039007094034 32-33 0.025179396077647607 34-35 0.02596123626019434 36-37 0.024894619852216675 38-39 0.023377539233102804 40-41 0.0244803998879535 42-43 0.0306885216023478 44-45 0.040112025789334975 46-47 0.044601134652037105 48-49 0.040909399220541584 50-51 0.028772754267630627 52-53 0.04335847475924759 54-55 0.04162910640844884 56-57 0.04443544666633184 58-59 0.047780272877756956 60-61 0.024252578907608752 62-63 0.025484883301291696 64-65 0.026484188965076597 66-67 0.03199849223933009 68-69 0.036218358125261155 70-71 0.039630495080879034 72-73 0.0433532970096943 74-75 0.025189751576754186 76-77 0.01857776539720329 78-79 0.01796679094991511 80-81 0.021104507179208642 82-83 0.0190230518587862 84-85 0.011686180741774755 86-87 0.013291283103294551 88-89 0.018971274363253306 90-91 0.019867025035972416 92-93 0.020146623511850058 94-95 0.02400922467860414 96-97 0.0246305546249989 98-99 0.02994810341622738 100 0.03031054588495765 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 9656705.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.56310953410207 #Duplication Level Percentage of deduplicated Percentage of total 1 75.99472187223286 46.02476664761661 2 14.262682303037971 17.275847811379755 3 4.6834018946956375 8.50924145822019 4 1.9383651267352673 4.695736779502065 5 0.9628998542681795 2.915810467220734 6 0.551506236613709 2.004055957006589 7 0.36427867498721583 1.5443294505931826 8 0.2340488693807641 1.133978185011197 9 0.17358595386025363 0.9461614623498116 >10 0.7685859763199192 8.051549734685775 >50 0.04063269004942148 1.7108099291304 >100 0.022605895650473978 2.6169947374266935 >500 0.001702573150042473 0.7118920790113041 >1k 9.166070870540636E-4 1.0109632789609362 >5k 0.0 0.0 >10k+ 6.547193119192035E-5 0.8478620218847244 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 28073 0.29070992641900106 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22546 0.23347508285693724 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13529 0.14009954741291156 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 1.0355499106579315E-5 0.0 3 0.0 0.0 0.0 1.0355499106579315E-5 0.0 4 0.0 0.0 0.0 1.0355499106579315E-5 0.0 5 0.0 0.0 0.0 1.0355499106579315E-5 0.0 6 0.0 0.0 0.0 1.0355499106579315E-5 0.0 7 0.0 0.0 0.0 2.071099821315863E-5 0.0 8 0.0 0.0 0.0 2.071099821315863E-5 0.0 9 0.0 0.0 0.0 4.142199642631726E-5 1.0355499106579315E-5 10-11 1.0355499106579315E-5 0.0 0.0 7.24884937460552E-5 1.0355499106579315E-5 12-13 1.0355499106579315E-5 0.0 0.0 8.802174240592417E-5 2.588874776644829E-5 14-15 1.0355499106579315E-5 0.0 0.0 1.0355499106579314E-4 8.284399285263452E-5 16-17 1.0355499106579315E-5 0.0 0.0 1.5015473704540008E-4 1.0355499106579314E-4 18-19 1.0355499106579315E-5 0.0 0.0 2.071099821315863E-4 1.1391049017237246E-4 20-21 1.0355499106579315E-5 0.0 0.0 3.003094740908001E-4 1.1908823972566211E-4 22-23 1.0355499106579315E-5 0.0 0.0 9.941279142316143E-4 1.3979923793882074E-4 24-25 1.0355499106579315E-5 0.0 0.0 0.002811518007436284 1.5533248659868972E-4 26-27 2.071099821315863E-5 0.0 0.0 0.006104566723328506 1.6568798570526904E-4 28-29 2.588874776644829E-5 0.0 1.0355499106579315E-5 0.01830334467087894 1.6568798570526904E-4 30-31 3.1066497319737946E-5 0.0 1.0355499106579315E-5 0.05938878737623238 1.6568798570526904E-4 32-33 3.1066497319737946E-5 0.0 1.0355499106579315E-5 0.15844949182977008 2.071099821315863E-4 34-35 3.1066497319737946E-5 0.0 1.0355499106579315E-5 0.30631048582306286 2.174654812381656E-4 36-37 3.1066497319737946E-5 0.0 1.0355499106579315E-5 0.5345042641356446 2.3817647945132423E-4 38-39 3.1066497319737946E-5 0.0 1.0355499106579315E-5 0.8869536762280716 2.433542290046139E-4 40-41 3.1066497319737946E-5 0.0 1.0355499106579315E-5 1.2814567701923172 2.5888747766448285E-4 42-43 3.1066497319737946E-5 0.0 1.0355499106579315E-5 1.6545084477572836 2.6406522721777255E-4 44-45 3.1066497319737946E-5 0.0 1.0355499106579315E-5 2.0914173105629716 2.7442072632435184E-4 46-47 3.1066497319737946E-5 0.0 1.0355499106579315E-5 2.58514679696646 2.847762254309311E-4 48-49 3.1066497319737946E-5 0.0 1.0355499106579315E-5 3.1107763983677663 3.4173147051711736E-4 50-51 3.1066497319737946E-5 0.0 1.0355499106579315E-5 3.658949921324096 3.520869696236967E-4 52-53 3.1066497319737946E-5 0.0 1.0355499106579315E-5 4.200780701077646 4.193977138164623E-4 54-55 3.1066497319737946E-5 0.0 1.0355499106579315E-5 4.788123899404611 4.867084580092278E-4 56-57 3.1066497319737946E-5 0.0 1.0355499106579315E-5 5.414118998146884 4.867084580092278E-4 58-59 3.1066497319737946E-5 0.0 1.0355499106579315E-5 6.137103701521378 5.074194562223864E-4 60-61 3.1066497319737946E-5 0.0 1.0355499106579315E-5 6.848428112901864 5.229527048822554E-4 62-63 3.1066497319737946E-5 0.0 1.0355499106579315E-5 7.583212907508306 5.384859535421244E-4 64-65 3.1066497319737946E-5 0.0 1.0355499106579315E-5 8.275674777266158 5.384859535421244E-4 66-67 3.1066497319737946E-5 0.0 1.0355499106579315E-5 9.034152953828453 5.43663703095414E-4 68-69 4.142199642631726E-5 0.0 1.0355499106579315E-5 9.845350976342345 5.488414526487037E-4 70-71 4.142199642631726E-5 0.0 1.0355499106579315E-5 10.684586512687298 5.695524508618622E-4 72-73 4.142199642631726E-5 0.0 1.0355499106579315E-5 11.468347640318306 6.627519428210761E-4 74-75 4.142199642631726E-5 0.0 1.0355499106579315E-5 12.221363291101882 6.627519428210761E-4 76-77 4.142199642631726E-5 5.177749553289657E-6 1.0355499106579315E-5 13.033633107773305 6.679296923743658E-4 78-79 4.142199642631726E-5 1.0355499106579315E-5 1.0355499106579315E-5 13.900041473773921 6.731074419276554E-4 80-81 4.142199642631726E-5 1.0355499106579315E-5 1.0355499106579315E-5 14.754577260048848 6.938184401408141E-4 82-83 4.142199642631726E-5 1.0355499106579315E-5 1.0355499106579315E-5 15.611795120592376 7.352404365671314E-4 84-85 4.142199642631726E-5 1.0355499106579315E-5 1.0355499106579315E-5 16.443041389376603 7.352404365671314E-4 86-87 4.142199642631726E-5 1.0355499106579315E-5 1.0355499106579315E-5 17.267002564539354 7.352404365671314E-4 88 4.142199642631726E-5 1.0355499106579315E-5 1.0355499106579315E-5 17.8984653668099 7.559514347802899E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 17110 0.0 48.55399 1 GTATCAA 30035 0.0 40.132435 1 TACCTGG 6770 0.0 35.735233 2 CAACGCA 36300 0.0 32.15442 5 ATCAACG 36585 0.0 31.981194 3 TATCAAC 38060 0.0 31.262989 2 AACGCAG 39020 0.0 30.527567 6 TCAACGC 38540 0.0 30.456476 4 GTACATG 37425 0.0 29.475573 1 TACATGG 38355 0.0 28.285975 2 ACCTGGG 8365 0.0 27.9857 3 GTACTAG 4320 0.0 27.596844 1 ACGCAGA 42535 0.0 27.496458 7 CGCAGAG 43035 0.0 27.078684 8 ACATGGG 38605 0.0 26.922663 3 CATGGGG 29490 0.0 25.584272 4 ATGGGGG 23520 0.0 25.082855 5 TGGGGGG 23915 0.0 24.884912 6 GCAGAGT 46430 0.0 24.298325 9 GCATCGT 4130 0.0 23.674788 8 >>END_MODULE