FastQCFastQC Report
Mon 6 Jun 2016
ERR1211415_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211415_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9656705
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT265940.2753941432403703No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT224200.23217028996950825No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135640.14046198988164182No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA172150.049.3303381
GTATCAA293200.038.843071
TACCTGG69850.033.5041472
CAACGCA367450.030.4730875
ATCAACG366950.030.3903353
TATCAAC380150.029.8042262
TCAACGC388800.028.8882184
AACGCAG397550.028.767016
ACCTGGG82950.027.5903513
GTACATG381400.027.2221091
TACATGG388350.026.4159412
ACGCAGA436450.025.7509127
TGGGGGG223100.025.6726136
ACATGGG385900.025.3664173
CGCAGAG442350.025.2056358
ATGGGGG229850.024.695235
CATGGGG290650.024.641314
GTACTAG39450.024.3156851
GCAGAGT482500.022.747799
GAGTACT253150.021.5108112-13
Produced by FastQC (version 0.11.5)