##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211415_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9656705 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.10145955582158 33.0 31.0 34.0 30.0 34.0 2 32.238920729172115 33.0 31.0 34.0 30.0 34.0 3 32.3136679643833 34.0 31.0 34.0 30.0 34.0 4 35.95122974140765 37.0 35.0 37.0 35.0 37.0 5 35.81134455282625 37.0 35.0 37.0 35.0 37.0 6 35.84760070852325 37.0 35.0 37.0 35.0 37.0 7 35.779507813482965 37.0 35.0 37.0 33.0 37.0 8 35.7606247679721 37.0 35.0 37.0 33.0 37.0 9 37.35209929266763 39.0 37.0 39.0 34.0 39.0 10-11 37.32701692761661 39.0 37.0 39.0 34.0 39.0 12-13 37.24515064921213 39.0 37.0 39.0 33.5 39.0 14-15 38.45960967017217 40.0 38.0 41.0 34.0 41.0 16-17 38.44561835532927 40.0 38.0 41.0 34.0 41.0 18-19 38.453506915661194 40.0 38.0 41.0 34.0 41.0 20-21 38.41697561435293 40.0 38.0 41.0 34.0 41.0 22-23 38.324266455276415 40.0 38.0 41.0 34.0 41.0 24-25 38.21065565324818 40.0 37.5 41.0 33.5 41.0 26-27 38.0857653309281 40.0 37.0 41.0 33.0 41.0 28-29 38.018301946678505 40.0 37.0 41.0 33.0 41.0 30-31 38.06424551645722 40.0 37.0 41.0 33.0 41.0 32-33 37.91404537054824 40.0 37.0 41.0 33.0 41.0 34-35 37.82217107180969 40.0 37.0 41.0 33.0 41.0 36-37 37.6922677041496 40.0 37.0 41.0 33.0 41.0 38-39 37.587966910038155 40.0 37.0 41.0 32.5 41.0 40-41 37.523457276576224 40.0 37.0 41.0 32.0 41.0 42-43 37.400343595460356 40.0 36.0 41.0 32.0 41.0 44-45 37.25188187896389 40.0 36.0 41.0 31.0 41.0 46-47 37.0347377806405 39.5 36.0 41.0 31.0 41.0 48-49 36.824865210234755 39.0 35.5 41.0 30.5 41.0 50-51 36.64931397407294 39.0 35.0 41.0 30.0 41.0 52-53 36.45403473545065 39.0 35.0 40.5 30.0 41.0 54-55 36.264428498126435 39.0 35.0 40.0 29.5 41.0 56-57 36.03945931868065 38.0 35.0 40.0 29.0 41.0 58-59 35.78255377999017 38.0 34.5 40.0 28.0 41.0 60-61 35.52790196034776 38.0 34.0 40.0 28.0 41.0 62-63 35.2354923858604 37.0 34.0 40.0 28.0 41.0 64-65 34.89342109964009 37.0 34.0 40.0 27.0 41.0 66-67 34.55102371875293 36.0 33.5 39.0 26.5 41.0 68-69 34.19017884464732 36.0 33.0 39.0 26.0 40.0 70-71 33.82587911715228 35.0 33.0 38.5 26.0 40.0 72-73 33.471004861389055 35.0 32.5 37.5 26.0 40.0 74-75 33.10166086672421 35.0 32.0 37.0 25.0 39.0 76-77 32.082574905208354 34.0 30.5 36.0 24.5 39.0 78-79 32.560148415013195 35.0 32.0 36.0 25.0 38.5 80-81 32.452689711449196 35.0 32.0 36.0 25.0 37.5 82-83 32.181727462938966 35.0 32.0 35.0 25.0 37.0 84-85 31.90534711374118 34.0 31.0 35.0 24.5 37.0 86-87 31.652720208394065 34.0 31.0 35.0 24.0 36.0 88-89 31.43024722200792 34.0 31.0 35.0 23.5 36.0 90-91 31.215015629037026 34.0 31.0 35.0 23.0 35.5 92-93 31.032360520488098 34.0 31.0 35.0 23.0 35.0 94-95 30.86845357707417 34.0 30.0 35.0 20.5 35.0 96-97 30.698259085267697 34.0 30.0 35.0 20.0 35.0 98-99 30.516342479137553 34.0 30.0 35.0 20.0 35.0 100 28.700407126447374 32.0 26.0 34.0 17.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.2291196227116039 1101 2 -0.1847537261265373 1101 3 -0.23229814785504743 1101 4 -0.14584677437695603 1101 5 -0.09348744210002025 1101 6 -0.18931521342169333 1101 7 -0.19711114099037275 1101 8 -0.27905889481690593 1101 9 -0.359078431130861 1101 10-11 -0.30490083020124814 1101 12-13 -0.37376474129627013 1101 14-15 -0.5199966221630774 1101 16-17 -0.46765781014550356 1101 18-19 -0.48748563672536704 1101 20-21 -0.5009274920029654 1101 22-23 -0.5350998658053214 1101 24-25 -0.5870023451282975 1101 26-27 -0.5751758274581391 1101 28-29 -0.6309426977579022 1101 30-31 -0.5184848453953208 1101 32-33 -0.4134954454287154 1101 34-35 -0.5130709539119778 1101 36-37 -0.5268980448865648 1101 38-39 -0.568994730907896 1101 40-41 -0.5306721381282244 1101 42-43 -0.5489678897673826 1101 44-45 -0.6307855099456887 1101 46-47 -0.6449889043720063 1101 48-49 -0.6382407889508173 1101 50-51 -0.7387015515116531 1101 52-53 -0.7218356195121913 1101 54-55 -0.6761532889594051 1101 56-57 -0.7572121010829633 1101 58-59 -0.6752277485367202 1101 60-61 -0.6975016250310802 1101 62-63 -0.7585405965741003 1101 64-65 -0.7127431168324989 1101 66-67 -0.7105021833142615 1101 68-69 -0.8091042578749352 1101 70-71 -0.7666748973312352 1101 72-73 -0.9018745799716754 1101 74-75 -0.8945846482304063 1101 76-77 -0.7194798972498049 1101 78-79 -0.6575195587713445 1101 80-81 -0.5393140486159957 1101 82-83 -0.6083987798813908 1101 84-85 -0.7142522538462757 1101 86-87 -0.7466175575704384 1101 88-89 -0.7924927473001269 1101 90-91 -0.8788831268689599 1101 92-93 -0.9963932057291558 1101 94-95 -0.965942454104848 1101 96-97 -0.9455018925586565 1101 98-99 -1.0395590265626709 1101 100 -0.9818519389369804 1102 1 -0.3371840634727121 1102 2 -0.27877203581727983 1102 3 -0.3116646177054001 1102 4 -0.18103190143936132 1102 5 -0.20544820524154517 1102 6 -0.288401063973474 1102 7 -0.27105217245431845 1102 8 -0.319941689584347 1102 9 -0.4430792113381017 1102 10-11 -0.4841261553861713 1102 12-13 -0.43568284307618654 1102 14-15 -0.6081779288802025 1102 16-17 -0.602400768049101 1102 18-19 -0.6680882993845785 1102 20-21 -0.6486906558920467 1102 22-23 -0.7043821161375448 1102 24-25 -0.8412836327911251 1102 26-27 -0.8511030203739267 1102 28-29 -0.785249305265296 1102 30-31 -0.7303696372918509 1102 32-33 -0.6317836576357152 1102 34-35 -0.6634586362174986 1102 36-37 -0.6391909406792422 1102 38-39 -0.7115035878761873 1102 40-41 -0.7328373361775462 1102 42-43 -0.8243785847900966 1102 44-45 -0.730428277036971 1102 46-47 -0.7694825806764101 1102 48-49 -0.8098812133123445 1102 50-51 -0.7864408184798961 1102 52-53 -0.8611577128801073 1102 54-55 -0.864335787301961 1102 56-57 -0.8731692304403182 1102 58-59 -0.8939144233068319 1102 60-61 -0.9387955715621743 1102 62-63 -0.962116670351584 1102 64-65 -1.0594857252707044 1102 66-67 -1.0093539658395159 1102 68-69 -1.0016663895763074 1102 70-71 -0.9807101485979004 1102 72-73 -0.9436358398365456 1102 74-75 -0.9569816253314087 1102 76-77 -0.7328071444123587 1102 78-79 -0.6804951511204429 1102 80-81 -0.6789554946719036 1102 82-83 -0.6562033081048781 1102 84-85 -0.6855262929130888 1102 86-87 -0.7814370869234857 1102 88-89 -0.8290731154965769 1102 90-91 -0.8656147582996248 1102 92-93 -0.9598702668517518 1102 94-95 -0.970795326718509 1102 96-97 -1.062156005680741 1102 98-99 -1.0856907264107853 1102 100 -1.003180570258845 1103 1 -0.314390214100726 1103 2 -0.263598194502606 1103 3 -0.33602693654180626 1103 4 -0.20562591846569234 1103 5 -0.18163241883698333 1103 6 -0.25500746876110014 1103 7 -0.2989887079990865 1103 8 -0.3034516378338097 1103 9 -0.45577535641123035 1103 10-11 -0.4722867263209096 1103 12-13 -0.5429267951227814 1103 14-15 -0.6927304181959073 1103 16-17 -0.7738213031434853 1103 18-19 -0.6994320020230163 1103 20-21 -0.6950382417645002 1103 22-23 -0.7962713548422613 1103 24-25 -0.7340812343721765 1103 26-27 -0.7889481379378651 1103 28-29 -0.8008075248348945 1103 30-31 -0.7230853698753918 1103 32-33 -0.7752750147403376 1103 34-35 -0.7552478784066068 1103 36-37 -0.6868428583518096 1103 38-39 -0.7701728178324672 1103 40-41 -0.8705238127610784 1103 42-43 -0.8451366951626298 1103 44-45 -0.8326893789539369 1103 46-47 -0.8846931953677384 1103 48-49 -0.9083143276660266 1103 50-51 -0.9396487412563843 1103 52-53 -0.9599237457987684 1103 54-55 -0.9168331804813548 1103 56-57 -0.9577450162804624 1103 58-59 -1.0210001173690344 1103 60-61 -0.9778927405305069 1103 62-63 -1.043224643263109 1103 64-65 -1.002521576289297 1103 66-67 -1.0560670393364546 1103 68-69 -0.9994867966981857 1103 70-71 -0.973354854138762 1103 72-73 -1.0228224110172164 1103 74-75 -1.0446307670027863 1103 76-77 -0.9597914741160629 1103 78-79 -0.761986825564616 1103 80-81 -0.7238027296566614 1103 82-83 -0.7129333543725238 1103 84-85 -0.7623291602573801 1103 86-87 -0.8247718476128867 1103 88-89 -0.817756778355264 1103 90-91 -1.0176458178364989 1103 92-93 -0.9424897925644657 1103 94-95 -0.9983287517597113 1103 96-97 -1.0718953063415384 1103 98-99 -1.2073512387442662 1103 100 -1.2658303958516761 1104 1 -0.3920112479478881 1104 2 -0.2951025522306523 1104 3 -0.3290259096953676 1104 4 -0.17552151133381955 1104 5 -0.23183274804815568 1104 6 -0.3120223142799716 1104 7 -0.3331288597995581 1104 8 -0.34997811097628784 1104 9 -0.517480325816301 1104 10-11 -0.4469256823492884 1104 12-13 -0.5679252027907893 1104 14-15 -0.6597614611927511 1104 16-17 -0.6726334373225171 1104 18-19 -0.7016257370412546 1104 20-21 -0.6789475608113449 1104 22-23 -0.7503440693058394 1104 24-25 -0.7892999294021124 1104 26-27 -0.7971009054385547 1104 28-29 -0.8380158788454679 1104 30-31 -0.8615454214503373 1104 32-33 -0.7203583643200702 1104 34-35 -0.7395359924652141 1104 36-37 -0.7698365034240453 1104 38-39 -0.9122444860647008 1104 40-41 -0.9384429477731118 1104 42-43 -0.7467244717022368 1104 44-45 -0.7573584923656327 1104 46-47 -0.7689428303775472 1104 48-49 -0.7611534556108737 1104 50-51 -0.7387108992511671 1104 52-53 -0.7884362363278541 1104 54-55 -0.7459041503029198 1104 56-57 -0.7552950542748889 1104 58-59 -0.8579586275666102 1104 60-61 -0.8289738647224425 1104 62-63 -0.8599407922234192 1104 64-65 -0.8633158010257702 1104 66-67 -0.889895412532546 1104 68-69 -0.8057059885356992 1104 70-71 -0.7921567226226642 1104 72-73 -0.7790867281588021 1104 74-75 -0.739384185393412 1104 76-77 -0.6972821693586724 1104 78-79 -0.7262540786894647 1104 80-81 -0.8185572972955981 1104 82-83 -0.7545705652050358 1104 84-85 -0.7096702566014947 1104 86-87 -0.6814770745603518 1104 88-89 -0.6928143376952463 1104 90-91 -0.6955543022244406 1104 92-93 -0.9028579784912409 1104 94-95 -0.9312129551617083 1104 96-97 -1.0325896572460955 1104 98-99 -1.0006892955343005 1104 100 -1.0533006538593064 1105 1 -0.35793470976271635 1105 2 -0.3372871841520535 1105 3 -0.37222343799339086 1105 4 -0.22202321359571897 1105 5 -0.26312447273451767 1105 6 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68-69 -0.9699003030062059 1105 70-71 -0.930469398419163 1105 72-73 -0.9846790260080951 1105 74-75 -1.0046211194855736 1105 76-77 -0.9859799863862619 1105 78-79 -0.9046703775470952 1105 80-81 -0.7525014194903292 1105 82-83 -0.779988217469338 1105 84-85 -0.7400568188411434 1105 86-87 -0.7907855783094142 1105 88-89 -0.8368470731204027 1105 90-91 -0.8207698227337197 1105 92-93 -0.84677244483224 1105 94-95 -0.8741540911851331 1105 96-97 -0.9657672693114954 1105 98-99 -0.9879549080958689 1105 100 -1.0239414271069833 1106 1 -0.34745518114348783 1106 2 -0.34716862702131124 1106 3 -0.3933678921732522 1106 4 -0.23791473774138439 1106 5 -0.2973753525216978 1106 6 -0.34376253306403015 1106 7 -0.38682541112420665 1106 8 -0.3996584372338674 1106 9 -0.5663588423481443 1106 10-11 -0.4799750491075585 1106 12-13 -0.5229536150678484 1106 14-15 -0.6798081229344177 1106 16-17 -0.6960109550071039 1106 18-19 -0.6830235485638951 1106 20-21 -0.6876532930987054 1106 22-23 -0.6670516292613229 1106 24-25 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0.7554815169347826 2201 96-97 0.7805687747272749 2201 98-99 0.805644767769877 2201 100 0.9521512065185931 2202 1 0.6311697664658382 2202 2 0.5968174950286098 2202 3 0.5654212692553884 2202 4 0.38089188179016986 2202 5 0.3583729494541288 2202 6 0.42687785490258534 2202 7 0.48649583630977133 2202 8 0.4879933346607075 2202 9 0.6494268426828853 2202 10-11 0.6753052476916253 2202 12-13 0.6977396350701071 2202 14-15 0.83101507997646 2202 16-17 0.817588814684342 2202 18-19 0.8150360468385145 2202 20-21 0.785967337783994 2202 22-23 0.7641988581466492 2202 24-25 0.7490389923736771 2202 26-27 0.7956585114583703 2202 28-29 0.8638394764029442 2202 30-31 0.995504383412964 2202 32-33 0.5962121868165795 2202 34-35 0.9384942519756194 2202 36-37 0.9992551168834467 2202 38-39 1.0349589714727543 2202 40-41 1.039565744679912 2202 42-43 1.0725361618497402 2202 44-45 1.0265019143279162 2202 46-47 1.1019053534005039 2202 48-49 1.1000367832284041 2202 50-51 1.1205136355404974 2202 52-53 1.0560941029286184 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1.139066833986611 2205 64-65 1.1549592183928468 2205 66-67 1.2182435444945767 2205 68-69 1.2094067014645802 2205 70-71 1.2014514914026933 2205 72-73 1.1940757066483059 2205 74-75 1.203280593953842 2205 76-77 1.1819571464219472 2205 78-79 1.1205376514926257 2205 80-81 1.025630547503411 2205 82-83 0.9566117388116595 2205 84-85 1.036875378128343 2205 86-87 1.07050272630822 2205 88-89 1.0755153759503742 2205 90-91 1.1289449593770335 2205 92-93 1.1574606328929136 2205 94-95 1.1728968657671324 2205 96-97 1.1682456290947414 2205 98-99 1.2559023005458805 2205 100 1.4982611991002663 2206 1 0.6581133260217413 2206 2 0.6490052880726651 2206 3 0.5834768212685546 2206 4 0.2781723904657625 2206 5 0.42273233124223 2206 6 0.4800221777439333 2206 7 0.5233177275879299 2206 8 0.5635089434144263 2206 9 0.7239492791770772 2206 10-11 0.7211170610174022 2206 12-13 0.7307317301602723 2206 14-15 0.8930499821342934 2206 16-17 0.9134056440316343 2206 18-19 0.9181549707573708 2206 20-21 0.9011688160650451 2206 22-23 0.8777939342839076 2206 24-25 0.9047631230928985 2206 26-27 0.9438537639215383 2206 28-29 0.9803312199465211 2206 30-31 1.0625649565486839 2206 32-33 1.112056034666189 2206 34-35 1.1084301956805618 2206 36-37 1.1586766505787836 2206 38-39 1.1267703376074607 2206 40-41 1.1297221637846633 2206 42-43 1.1907001747009573 2206 44-45 1.204474417178119 2206 46-47 1.2558398059017506 2206 48-49 1.2633271360587628 2206 50-51 1.230326440168156 2206 52-53 1.2441413062415734 2206 54-55 1.2392020547832274 2206 56-57 1.2445097238195828 2206 58-59 1.244967936393401 2206 60-61 1.2368040482497236 2206 62-63 1.2326984827350742 2206 64-65 1.2457366511277428 2206 66-67 1.269229641246433 2206 68-69 1.2819294462673696 2206 70-71 1.2742698152881573 2206 72-73 1.2579293450343982 2206 74-75 1.2130649850657917 2206 76-77 1.20982581547565 2206 78-79 1.1566096478914716 2206 80-81 1.1012551947215883 2206 82-83 1.0641242273089304 2206 84-85 1.0832792558584288 2206 86-87 1.1453810942989264 2206 88-89 1.168743500759419 2206 90-91 1.1741675007831454 2206 92-93 1.2176107641561629 2206 94-95 1.201861832915494 2206 96-97 1.1678275079965488 2206 98-99 1.2483842984814437 2206 100 1.4765288667682945 2207 1 0.6843499944873699 2207 2 0.6277790185790764 2207 3 0.5993106674264155 2207 4 0.4025967598873663 2207 5 0.4847288686162088 2207 6 0.507534979867188 2207 7 0.532133067034259 2207 8 0.5694841920989759 2207 9 0.712989571849242 2207 10-11 0.7070139568573595 2207 12-13 0.7101212905973142 2207 14-15 0.8803605280036422 2207 16-17 0.8657921869069227 2207 18-19 0.8481899069963674 2207 20-21 0.8996201131793669 2207 22-23 0.8948618875038648 2207 24-25 0.9170627870759134 2207 26-27 0.9280899403366618 2207 28-29 0.9388825716823703 2207 30-31 1.011319396586714 2207 32-33 1.0607426382973202 2207 34-35 1.0285068191698983 2207 36-37 1.0546658352563085 2207 38-39 1.0743049785508916 2207 40-41 1.0786518397165423 2207 42-43 1.1570413092754706 2207 44-45 1.1564029419467303 2207 46-47 1.1402590492533662 2207 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1.000526000557521 2311 34-35 0.9925118507270199 2311 36-37 1.0635219425299596 2311 38-39 1.0849060630091785 2311 40-41 1.0458324486007626 2311 42-43 1.1285295188223472 2311 44-45 1.1394910569526218 2311 46-47 1.1638712701302936 2311 48-49 1.1629189276656362 2311 50-51 1.196839701619659 2311 52-53 1.2172782156517243 2311 54-55 1.1891512490447909 2311 56-57 1.2166081710975547 2311 58-59 1.2528286225231113 2311 60-61 1.2808314219200838 2311 62-63 1.293296945688418 2311 64-65 1.332482420644908 2311 66-67 1.3116428406170613 2311 68-69 1.3141830853825454 2311 70-71 1.3251601649669098 2311 72-73 1.2866546708316662 2311 74-75 1.2234926102424524 2311 76-77 1.1190845267302159 2311 78-79 1.0788001860408087 2311 80-81 1.0782780309660538 2311 82-83 1.1194282867232417 2311 84-85 1.145745758684214 2311 86-87 1.183596581925002 2311 88-89 1.2287586565448478 2311 90-91 1.269748435955659 2311 92-93 1.2845001074277462 2311 94-95 1.3440114471817743 2311 96-97 1.3854615909843986 2311 98-99 1.419462441606104 2311 100 1.476598856944527 2312 1 0.0817550155877882 2312 2 0.0810430853588926 2312 3 0.26547710674273617 2312 4 0.19505700690472594 2312 5 0.25927797386950857 2312 6 0.36538945162616443 2312 7 0.4203760673570329 2312 8 0.290733475792905 2312 9 0.34354200897513465 2312 10-11 0.23162897830425777 2312 12-13 0.37159005745169793 2312 14-15 0.7386834323355842 2312 16-17 0.7692585816586828 2312 18-19 0.7915832085025443 2312 20-21 0.8111984600068922 2312 22-23 0.4364196344597602 2312 24-25 0.40400704187832304 2312 26-27 0.681803318805521 2312 28-29 0.8289356891614688 2312 30-31 0.7900420910432615 2312 32-33 0.6482240935709243 2312 34-35 0.8256901901916436 2312 36-37 0.8738972298015 2312 38-39 0.8921734107717256 2312 40-41 0.9202143688584812 2312 42-43 0.7371974836498794 2312 44-45 0.7285911014427242 2312 46-47 0.8850377258473543 2312 48-49 0.9077367653613919 2312 50-51 0.9609716921747449 2312 52-53 0.9837766555078957 2312 54-55 0.997348356836973 2312 56-57 0.9774870730499856 2312 58-59 1.001390948007419 2312 60-61 1.0168705533367444 2312 62-63 1.042915583331954 2312 64-65 1.0606738594107838 2312 66-67 1.0544010770019767 2312 68-69 1.0223404912824847 2312 70-71 0.9421072175739056 2312 72-73 1.0419133294914928 2312 74-75 1.1240646031731742 2312 76-77 0.9720542644591141 2312 78-79 0.9468163551484139 2312 80-81 0.8179620829994008 2312 82-83 0.8150209266160786 2312 84-85 0.6855287514734272 2312 86-87 0.8856872448812041 2312 88-89 0.4937657778288127 2312 90-91 0.857014645741959 2312 92-93 1.0530853980733355 2312 94-95 1.17010668185522 2312 96-97 1.1773015092071333 2312 98-99 1.1580259527570824 2312 100 1.2395769118215405 2313 1 -0.4375181658716869 2313 2 -0.08362945632494245 2313 3 0.21072209834858313 2313 4 0.0848952610630036 2313 5 0.21353135487380115 2313 6 0.3215048752857328 2313 7 0.09183339453643669 2313 8 0.0723669256274917 2313 9 0.2623342287848658 2313 10-11 0.3787521140017489 2313 12-13 0.5427182418341019 2313 14-15 0.5537263888269166 2313 16-17 0.6974450752445662 2313 18-19 0.5139057314236766 2313 20-21 0.7553659042406125 2313 22-23 0.8029204548450366 2313 24-25 0.6753314318524417 2313 26-27 0.8308869493389608 2313 28-29 0.7168745239229679 2313 30-31 0.7235582046709368 2313 32-33 0.849314025519682 2313 34-35 0.8606747012937532 2313 36-37 0.7369798401312764 2313 38-39 0.5599003637139006 2313 40-41 0.7167106041255948 2313 42-43 0.7523845799617419 2313 44-45 0.9742554440313356 2313 46-47 1.0412561511239034 2313 48-49 1.0713544027826174 2313 50-51 0.9618667045652742 2313 52-53 0.941110213042208 2313 54-55 0.9905317550220687 2313 56-57 1.0764341015544332 2313 58-59 0.9401772044873908 2313 60-61 0.9632797100139783 2313 62-63 0.9777054722246064 2313 64-65 1.0546798804262068 2313 66-67 1.0572614904832989 2313 68-69 1.1000592255044168 2313 70-71 1.0212705836691285 2313 72-73 1.0570628334839967 2313 74-75 0.7763266542731557 2313 76-77 0.8950186667553659 2313 78-79 0.7440008710675698 2313 80-81 0.8434630710893884 2313 82-83 0.8992430949190506 2313 84-85 0.9414213939941902 2313 86-87 1.0345203070324516 2313 88-89 0.9903537526279109 2313 90-91 1.1196030401001842 2313 92-93 0.8692222040835844 2313 94-95 0.8693956540215915 2313 96-97 1.1094422806607014 2313 98-99 1.2005084552681922 2313 100 1.2717101306161247 2314 1 -0.42405611459229675 2314 2 -0.22381846833007657 2314 3 0.05732963400929236 2314 4 -0.2194398679320031 2314 5 0.12354667081154957 2314 6 0.16275920643206376 2314 7 0.15482621304416 2314 8 0.16361998693914614 2314 9 0.26705456501399993 2314 10-11 0.4229421153235293 2314 12-13 0.4886002617832048 2314 14-15 0.5692513226079257 2314 16-17 0.7657767260469583 2314 18-19 0.8093958125718785 2314 20-21 0.46058584826399596 2314 22-23 0.6896519667736953 2314 24-25 0.8726811930946639 2314 26-27 0.8158015731590993 2314 28-29 0.7553175421121949 2314 30-31 0.7507011516917004 2314 32-33 0.824228412688818 2314 34-35 0.8602342706812678 2314 36-37 0.7534374819097565 2314 38-39 0.736521639562973 2314 40-41 0.7606708519511471 2314 42-43 0.6453433827731629 2314 44-45 0.7164700154996524 2314 46-47 0.8235952327679215 2314 48-49 0.7130967689117398 2314 50-51 0.8578473820105259 2314 52-53 0.950516614394239 2314 54-55 0.9671299141081136 2314 56-57 0.8635713112312615 2314 58-59 0.8163899326752286 2314 60-61 0.8966279061849605 2314 62-63 0.6046630738878633 2314 64-65 0.8354316759435676 2314 66-67 0.9299220149019263 2314 68-69 0.9815466809808413 2314 70-71 1.030186201134896 2314 72-73 0.9772907514919211 2314 74-75 0.9981345929332122 2314 76-77 0.7984134326699994 2314 78-79 0.7856921335660587 2314 80-81 0.7717962283774682 2314 82-83 0.7710769786102745 2314 84-85 0.6541699497015543 2314 86-87 0.7853761828172239 2314 88-89 0.8496187766479864 2314 90-91 0.9577586716149362 2314 92-93 1.0400943178792446 2314 94-95 1.1080795439754105 2314 96-97 1.132801250224496 2314 98-99 1.1269686785628465 2314 100 1.138164720462413 2315 1 -0.11482572613134323 2315 2 0.08603704010742064 2315 3 0.10341429816138259 2315 4 -0.060665508156127146 2315 5 0.2550258021221623 2315 6 0.3389163598745242 2315 7 0.41912316017007356 2315 8 0.34375960789459015 2315 9 0.558857343090061 2315 10-11 0.5555979137130933 2315 12-13 0.632822233999903 2315 14-15 0.7071495943045747 2315 16-17 0.7279557467360647 2315 18-19 0.7888953060007253 2315 20-21 0.7735119812682072 2315 22-23 0.8558746698149804 2315 24-25 0.7971526668394731 2315 26-27 0.8567069659796545 2315 28-29 0.8736833462300595 2315 30-31 0.8854575824874047 2315 32-33 0.9396999718210424 2315 34-35 0.9412978102577014 2315 36-37 0.9896723591333938 2315 38-39 0.9789262036245319 2315 40-41 0.9552796491311426 2315 42-43 1.0240106524592818 2315 44-45 0.8732028262076383 2315 46-47 0.8742359483679891 2315 48-49 0.9838071349146631 2315 50-51 0.9158773469227057 2315 52-53 0.9419091931787804 2315 54-55 0.8630159219465199 2315 56-57 1.0131231928774227 2315 58-59 1.0900251468373696 2315 60-61 1.1078893093435482 2315 62-63 1.0750308478183186 2315 64-65 1.0864478329331178 2315 66-67 1.1082498812749932 2315 68-69 1.0189971633900328 2315 70-71 0.6101711035692645 2315 72-73 0.856247036997388 2315 74-75 1.0102078792860567 2315 76-77 0.9619853892337424 2315 78-79 0.8705302556220005 2315 80-81 0.9116614466085622 2315 82-83 0.8779579944479678 2315 84-85 0.8073108203874142 2315 86-87 0.8212036036768637 2315 88-89 0.9201110480675396 2315 90-91 1.0203103907659958 2315 92-93 1.0547149495601644 2315 94-95 1.0932749960317345 2315 96-97 1.188499726740556 2315 98-99 1.09957315915619 2315 100 1.0537756254081252 2316 1 0.4637482102196202 2316 2 0.4820271367631648 2316 3 0.471903376167198 2316 4 0.30502685363884297 2316 5 0.3783873078652604 2316 6 0.3990332556452856 2316 7 0.4384369652001183 2316 8 0.4480368390920546 2316 9 0.4863589543564757 2316 10-11 0.442417920619242 2316 12-13 0.5792020771762267 2316 14-15 0.7230927123721429 2316 16-17 0.7264262902998055 2316 18-19 0.7802562584423853 2316 20-21 0.8038789510798878 2316 22-23 0.8092786083441723 2316 24-25 0.8727946692414505 2316 26-27 0.9347928609700347 2316 28-29 0.8593420271727155 2316 30-31 0.745387832971943 2316 32-33 0.7479132316583659 2316 34-35 0.6332423887635557 2316 36-37 0.6833048105811201 2316 38-39 0.6655015321906319 2316 40-41 0.6981822355552225 2316 42-43 0.7939629294869661 2316 44-45 0.7169691728469374 2316 46-47 0.7782915554522774 2316 48-49 0.4403392935375692 2316 50-51 0.5610310232191082 2316 52-53 0.6911396228503079 2316 54-55 0.7674565852086701 2316 56-57 0.8097930061931109 2316 58-59 0.7050813492844128 2316 60-61 0.8304784478974341 2316 62-63 0.8553237767039121 2316 64-65 0.8311510339727732 2316 66-67 0.7717047833769186 2316 68-69 0.7828201236944139 2316 70-71 0.6592035415168951 2316 72-73 0.703089569111782 2316 74-75 0.8394801997163981 2316 76-77 0.6607504053767705 2316 78-79 0.6228901339824091 2316 80-81 0.6004952221638789 2316 82-83 0.5197113529573798 2316 84-85 0.5943090603164478 2316 86-87 0.6193149204692645 2316 88-89 0.5626601026201996 2316 90-91 0.6098637761888739 2316 92-93 0.6381596152962814 2316 94-95 0.5679108657202825 2316 96-97 0.6948551772439977 2316 98-99 0.820071156473805 2316 100 0.4912996101151528 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 8.0 12 42.0 13 251.0 14 899.0 15 2756.0 16 6480.0 17 12910.0 18 21519.0 19 31148.0 20 43448.0 21 59164.0 22 71518.0 23 75516.0 24 77354.0 25 84606.0 26 99699.0 27 122339.0 28 150589.0 29 184316.0 30 225421.0 31 281341.0 32 355438.0 33 463307.0 34 654025.0 35 890635.0 36 1355678.0 37 2120641.0 38 1953478.0 39 312142.0 40 36.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.891921714198745 20.414767747612533 13.981186649527139 23.712123888661583 2 15.405047580929521 20.90141513073041 40.854411520285645 22.839125768054423 3 17.82269417984706 27.187254865919584 31.028968990975702 23.961081963257655 4 12.393907587612919 17.758191433145804 38.83631178207081 31.01158919717047 5 12.635744323108538 38.374337491897776 34.659310913904164 14.33060727108953 6 29.18954239567223 38.02235855812101 19.03368695636866 13.754412089838098 7 26.228001383911483 33.30102756773623 23.23352719208979 17.237443856262505 8 25.664321376575483 35.90946931024488 20.5705410121658 17.85566830101384 9 27.155899990514186 15.61621966662124 20.777642141209405 36.450238201655175 10-11 24.69597022560689 26.339935712495677 28.872876845107292 20.091217216790138 12-13 25.11470683643166 24.27279761967943 28.54049514416571 22.072000399723198 14-15 22.95130884229419 25.68868750428459 26.333167369149425 25.02683628427179 16-17 21.96486996845194 28.1882155333683 27.2691329694062 22.57778152877356 18-19 21.592157987636572 27.73708526873297 29.155146605389724 21.515610138240735 20-21 22.705059474482212 27.060256425988804 28.56521601132146 21.669468088207523 22-23 22.59892712875946 26.85013955072041 28.435105808358628 22.1158275121615 24-25 22.124189532873007 26.730478669238906 28.859549787171073 22.285782010717014 26-27 22.11630157491608 26.925436782007946 28.563019166475524 22.395242476600455 28-29 21.832080364919275 27.740722240635073 28.538637532055144 21.88855986239051 30-31 22.864826260905666 26.928794602625217 28.654859119709634 21.551520016759486 32-33 22.79922441123335 27.039137632621085 28.21610389115409 21.945534064991474 34-35 22.712046652692514 27.180977226039154 28.620783840390963 21.48619228087737 36-37 22.369267807113175 27.119528418720073 28.553636787259563 21.95756698690719 38-39 22.375071771492102 27.059101904358503 28.70706392024629 21.858762403903107 40-41 22.31580159509165 26.437955710791687 29.137240812363537 22.109001881753127 42-43 22.164954111871264 26.81893533576457 28.44585030975937 22.570260242604796 44-45 21.606468954681493 27.24420643615567 28.864546875785635 22.284777733377194 46-47 21.631660078144634 26.661736042111166 28.484470422803472 23.22213345694073 48-49 22.024133460409686 26.74026373072379 28.522379083892453 22.71322372497408 50-51 21.903757560002973 26.88660830543305 28.381238272755354 22.828395861808623 52-53 21.484452031412864 27.244102423245124 28.15881614292556 23.112629402416452 54-55 21.398523427668575 26.890349482240666 28.478073303747482 23.233053786343273 56-57 21.90545505533822 26.711577508016322 27.98072654779997 23.402240888845487 58-59 21.36744918310404 26.92884340026069 28.479375704617944 23.224331712017328 60-61 21.881758038969384 27.21851633424135 27.942449455318176 22.95727617147109 62-63 21.81897289293807 26.978248447331037 27.78498183314662 23.41779682658427 64-65 21.2951760526436 26.684660114009485 27.906180659569618 24.1139831737773 66-67 21.48563626386679 26.542104235580926 27.7717926970642 24.20046680348808 68-69 21.141934838796246 26.781499270305176 27.925532273130187 24.15103361776839 70-71 21.443832582345735 26.451801432600664 27.93114909235505 24.173216892698548 72-73 21.50407062061878 26.8673703381591 27.529915189576393 24.09864385164573 74-75 21.692306903464978 26.997417978355724 27.320801260054324 23.989473858124974 76-77 21.536017319492586 26.80633269844065 27.32527524604789 24.33237473601887 78-79 21.408391189049542 26.781036892502037 27.54769957179779 24.262872346650635 80-81 21.37466022843916 27.08652980255348 27.258932025804828 24.27987794320254 82-83 21.85398647427684 26.637710029407057 27.526898115703734 23.98140538061237 84-85 22.03679328612069 26.95260067972402 26.861355393964498 24.149250640190786 86-87 21.345991862817925 27.23584980921033 27.146137739471342 24.272020588500403 88-89 21.560079010386925 26.574920657519108 27.215540795593522 24.649459536500444 90-91 21.992598775875692 26.26515186359687 27.26965624491105 24.47259311561639 92-93 21.936258071065254 26.544193468360806 27.06636620663688 24.453182253937065 94-95 21.396064190885202 26.34671058555777 27.29472400086931 24.96250122268772 96-97 21.41336642929815 26.254174255142736 27.389311721747013 24.9431475938121 98-99 21.33616370190582 26.480254486656218 27.170026059153525 25.013555752284432 100 21.31792299540213 26.51240240192963 27.12832773893971 25.041346863728524 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 974.0 1 712.5 2 760.0 3 1090.0 4 896.5 5 777.5 6 1144.0 7 2116.5 8 3432.5 9 4370.5 10 4320.5 11 4163.5 12 4661.0 13 5435.0 14 6430.0 15 7467.0 16 8490.5 17 9671.5 18 10835.0 19 11981.0 20 13158.0 21 14695.5 22 17015.0 23 20159.0 24 24457.5 25 30119.5 26 37674.0 27 46753.0 28 58482.5 29 75740.0 30 96907.5 31 117123.5 32 142510.0 33 168413.0 34 191508.0 35 215100.0 36 239955.5 37 273210.5 38 291870.5 39 292142.5 40 291761.5 41 293691.0 42 301067.5 43 314132.0 44 331258.0 45 358008.0 46 382956.0 47 402498.5 48 424095.0 49 435059.0 50 436256.5 51 432571.5 52 413875.0 53 384368.0 54 354705.5 55 313843.0 56 273214.0 57 234337.5 58 192544.0 59 158404.5 60 124171.0 61 93001.5 62 71357.0 63 54762.5 64 39107.5 65 26025.0 66 17958.5 67 12851.5 68 9361.0 69 6782.5 70 5027.5 71 3590.0 72 2517.5 73 1832.5 74 1274.5 75 846.0 76 538.5 77 305.5 78 177.5 79 115.0 80 73.0 81 45.0 82 31.5 83 21.0 84 13.0 85 8.0 86 7.0 87 6.5 88 5.0 89 2.5 90 1.0 91 1.5 92 2.5 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05117687658471497 2 0.0 3 0.0 4 0.017386882999946666 5 0.004225043635484361 6 0.0 7 1.8639898391842766E-4 8 4.763529589026485E-4 9 0.0018743453382908557 10-11 8.802174240592418E-4 12-13 2.3299872989803458E-4 14-15 3.106649731973794E-5 16-17 1.0355499106579314E-4 18-19 0.0 20-21 5.177749553289657E-6 22-23 1.0355499106579315E-5 24-25 5.695524508618623E-5 26-27 0.0 28-29 0.001071794157530959 30-31 8.75039674505952E-4 32-33 0.01377799156130378 34-35 0.0229270750219666 36-37 0.030771365595200433 38-39 0.020001646524357947 40-41 1.9157673347171733E-4 42-43 0.0018329233418645388 44-45 0.009584014423139156 46-47 0.014533942996084069 48-49 0.005881923492537051 50-51 0.01074383032307604 52-53 0.016082090112517676 54-55 0.009982701138742459 56-57 0.0032464489699126154 58-59 0.007901245818320017 60-61 0.0015533248659868972 62-63 0.0011080384044039866 64-65 0.019613315307861222 66-67 0.04622177026221677 68-69 0.05602325016659409 70-71 0.04916790975803859 72-73 0.041421996426317255 74-75 0.029363017716705648 76-77 0.0294355062104517 78-79 0.012633708910026763 80-81 0.002247143306127711 82-83 0.02826533481140824 84-85 0.04866566805136949 86-87 0.04882617828752147 88-89 0.06028971579850477 90-91 0.03559185042931311 92-93 0.027131407659237803 94-95 0.007492203603610135 96-97 0.003536402944896836 98-99 0.0220779240952271 100 0.018650253890949344 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 9656705.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.64681687466263 #Duplication Level Percentage of deduplicated Percentage of total 1 74.15958288812406 43.49223477141177 2 15.2576045584259 17.896198809680435 3 5.061182410698419 8.904667140284813 4 2.127566169538977 4.990999341347193 5 1.0976264377060052 3.218614834446618 6 0.585447721852803 2.060078719991386 7 0.38163573817444846 1.5667204874680503 8 0.2464377892069426 1.156223351569302 9 0.17917118288396622 0.945703759663139 >10 0.8316472823721216 8.488641728637388 >50 0.04435205702078648 1.7875961376410405 >100 0.024799458373230252 2.7472407455279835 >500 0.0019265391124927546 0.7940337886285485 >1k 9.291206018513309E-4 1.0247170103792502 >5k 2.2661476989933095E-5 0.09465305303884745 >10k+ 6.798443096979928E-5 0.8316763202843297 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26594 0.2753941432403703 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22420 0.23217028996950825 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13564 0.14046198988164182 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.0355499106579315E-5 0.0 5 0.0 0.0 0.0 1.0355499106579315E-5 0.0 6 0.0 0.0 0.0 1.0355499106579315E-5 1.0355499106579315E-5 7 0.0 0.0 0.0 1.0355499106579315E-5 1.0355499106579315E-5 8 0.0 0.0 0.0 1.0355499106579315E-5 1.0355499106579315E-5 9 0.0 0.0 0.0 3.1066497319737946E-5 1.0355499106579315E-5 10-11 0.0 0.0 0.0 5.177749553289657E-5 1.0355499106579315E-5 12-13 0.0 0.0 0.0 6.731074419276555E-5 2.071099821315863E-5 14-15 0.0 0.0 0.0 8.802174240592417E-5 7.766624329934486E-5 16-17 0.0 0.0 0.0 1.5015473704540008E-4 1.0355499106579314E-4 18-19 0.0 0.0 0.0 2.174654812381656E-4 1.346214883855311E-4 20-21 0.0 0.0 0.0 3.054872236440898E-4 1.346214883855311E-4 22-23 0.0 0.0 0.0 0.001009661162891483 1.6568798570526904E-4 24-25 0.0 0.0 0.0 0.002842584504756022 2.0193223257829662E-4 26-27 0.0 0.0 0.0 0.006223654963054168 2.071099821315863E-4 28-29 0.0 0.0 0.0 0.01853634340077697 2.1228773168487596E-4 30-31 0.0 0.0 0.0 0.059916917830667916 2.2782098034474491E-4 32-33 0.0 0.0 0.0 0.15997175019843726 2.5888747766448285E-4 34-35 1.0355499106579315E-5 0.0 0.0 0.30946891305056956 2.692429767710622E-4 36-37 1.0355499106579315E-5 0.0 0.0 0.5397803909304467 2.7442072632435184E-4 38-39 2.071099821315863E-5 0.0 0.0 0.8964030691628251 2.795984758776415E-4 40-41 2.071099821315863E-5 0.0 0.0 1.295483293732179 3.003094740908001E-4 42-43 2.071099821315863E-5 0.0 0.0 1.6731121019022535 3.054872236440898E-4 44-45 2.071099821315863E-5 0.0 0.0 2.1142926080894053 3.158427227506691E-4 46-47 2.071099821315863E-5 0.0 0.0 2.6130393338100317 3.520869696236967E-4 48-49 2.071099821315863E-5 0.0 0.0 3.1424901143816655 4.038644651565933E-4 50-51 2.071099821315863E-5 0.0 0.0 3.6942000402828916 4.038644651565933E-4 52-53 2.071099821315863E-5 0.0 0.0 4.238407407081401 4.4528646158291054E-4 54-55 2.071099821315863E-5 0.0 0.0 4.828049526210027 4.918862075625175E-4 56-57 2.071099821315863E-5 0.0 0.0 5.4566956327235845 5.125972057756761E-4 58-59 2.071099821315863E-5 0.0 0.0 6.181875701908673 5.488414526487037E-4 60-61 2.071099821315863E-5 0.0 0.0 6.895737210570273 5.695524508618624E-4 62-63 2.071099821315863E-5 0.0 0.0 7.632769148482842 5.695524508618624E-4 64-65 2.071099821315863E-5 0.0 0.0 8.326484033632589 5.954411986283106E-4 66-67 2.071099821315863E-5 0.0 0.0 9.086437868817573 6.213299463947589E-4 68-69 2.071099821315863E-5 0.0 0.0 9.90077878531031 6.213299463947589E-4 70-71 2.071099821315863E-5 0.0 0.0 10.743447169609095 6.731074419276554E-4 72-73 2.071099821315863E-5 0.0 0.0 11.531485118371121 7.870179321000279E-4 74-75 2.071099821315863E-5 0.0 0.0 12.28847210306207 8.025511807598969E-4 76-77 2.071099821315863E-5 0.0 0.0 13.105013563114955 8.077289303131866E-4 78-79 2.071099821315863E-5 0.0 0.0 13.976682522661715 8.077289303131866E-4 80-81 2.071099821315863E-5 0.0 0.0 14.835810972790409 8.232621789730555E-4 82-83 2.071099821315863E-5 0.0 0.0 15.697776829674304 8.802174240592417E-4 84-85 2.071099821315863E-5 0.0 0.0 16.534501157485913 8.802174240592417E-4 86-87 2.071099821315863E-5 0.0 0.0 17.364225167901473 8.853951736125314E-4 88 2.071099821315863E-5 0.0 0.0 18.001015874462354 8.905729231658211E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 17215 0.0 49.330338 1 GTATCAA 29320 0.0 38.84307 1 TACCTGG 6985 0.0 33.504147 2 CAACGCA 36745 0.0 30.473087 5 ATCAACG 36695 0.0 30.390335 3 TATCAAC 38015 0.0 29.804226 2 TCAACGC 38880 0.0 28.888218 4 AACGCAG 39755 0.0 28.76701 6 ACCTGGG 8295 0.0 27.590351 3 GTACATG 38140 0.0 27.222109 1 TACATGG 38835 0.0 26.415941 2 ACGCAGA 43645 0.0 25.750912 7 TGGGGGG 22310 0.0 25.672613 6 ACATGGG 38590 0.0 25.366417 3 CGCAGAG 44235 0.0 25.205635 8 ATGGGGG 22985 0.0 24.69523 5 CATGGGG 29065 0.0 24.64131 4 GTACTAG 3945 0.0 24.315685 1 GCAGAGT 48250 0.0 22.74779 9 GAGTACT 25315 0.0 21.51081 12-13 >>END_MODULE