FastQCFastQC Report
Mon 6 Jun 2016
ERR1211414_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211414_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12120587
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300040.2475457665540456No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240280.1982412237955142No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142030.11718079330646279No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA175100.049.5114441
GTATCAA328900.042.3605271
ATCAACG387200.034.9531173
CAACGCA388100.034.847695
TATCAAC406200.033.8933452
AACGCAG418700.032.7278146
TCAACGC415250.032.6260074
ACGCAGA459950.029.2099767
CGCAGAG466650.028.688318
GTACTAG51650.026.2721791
TACCTGG80350.025.5404832
GCAGAGT508000.025.4742039
GTACATG390800.025.3064841
TACATGG397250.024.325042
ACATGGG392050.023.8971143
CATGGGG287850.022.079544
GCATCGT54600.021.436958
GTGGTAT79500.021.1580181
GAGTACT294650.021.15133512-13
CAGAGTA496800.020.80654110-11
Produced by FastQC (version 0.11.5)